AlfalfaGEDB | Msa0323630

M. sativa cultivar zhongmu-4 / Msa0323630

KEGG: K21407
GO: GO:0003674 GO:0003824 GO:0004064 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0009986 GO:0012505 GO:0016020 GO:0016787 GO:0016788 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0052689
PFAM: PF20067.2

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0323630	XP_003595316.1	94.609	371	20	0	1	371	1	371	0.0	701

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0323630	sp\|Q9SD07\|SSL4_ARATH	30.978	368	214	11	22	368	13	361	3.74e-47	167

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0003470	Msa0323630	0.820644	6.150385e-53	-8.615850e-47
Msa0004350	Msa0323630	0.848237	6.967061e-60	-8.615850e-47
Msa0005160	Msa0323630	0.806091	9.733775e-50	-8.615850e-47
Msa0005350	Msa0323630	0.817922	2.564633e-52	-8.615850e-47
Msa0006050	Msa0323630	0.808875	2.497638e-50	-8.615850e-47
Msa0006700	Msa0323630	0.882467	1.016875e-70	-8.615850e-47
Msa0006820	Msa0323630	0.846037	2.792282e-59	-8.615850e-47
Msa0007230	Msa0323630	0.827715	1.342000e-54	-8.615850e-47
Msa0008330	Msa0323630	0.857838	1.242578e-62	-8.615850e-47
Msa0008850	Msa0323630	0.895986	5.686095e-76	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0323630	MtrunA17_Chr2g0300861	94.609	371	20	0	1	371	1	371	0.0	701
Msa0323630	MtrunA17_Chr2g0300851	85.915	355	47	1	17	371	1	352	0.0	630
Msa0323630	MtrunA17_Chr2g0300841	85.836	353	49	1	20	371	8	360	0.0	612
Msa0323630	MtrunA17_Chr8g0370791	32.764	351	222	10	22	366	22	364	3.34e-55	185
Msa0323630	MtrunA17_Chr8g0370761	32.123	358	215	11	22	366	22	364	1.45e-50	173
Msa0323630	MtrunA17_Chr8g0370771	32.218	239	141	5	136	366	4	229	2.15e-36	132
Msa0323630	MtrunA17_Chr4g0025341	30.303	264	171	4	110	364	128	387	2.13e-33	128
Msa0323630	MtrunA17_Chr2g0329531	27.562	283	193	6	96	367	124	405	1.59e-31	123
Msa0323630	MtrunA17_Chr7g0256601	30.522	249	163	5	126	365	167	414	2.81e-30	120
Msa0323630	MtrunA17_Chr2g0322241	30.800	250	160	8	124	364	146	391	4.66e-28	113

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0323630	AT3G51430.2	30.532	357	212	9	31	368	20	359	2.41e-48	167
Msa0323630	AT3G51420.1	30.978	368	214	11	22	368	13	361	3.81e-48	167
Msa0323630	AT3G51430.1	30.641	359	211	10	31	368	20	361	4.91e-48	167
Msa0323630	AT3G51440.1	30.851	376	219	11	17	368	3	361	1.53e-46	163
Msa0323630	AT3G51450.1	30.245	367	226	11	20	368	7	361	6.72e-43	153
Msa0323630	AT3G57020.2	30.333	300	183	11	77	358	54	345	1.72e-32	125
Msa0323630	AT1G08470.1	29.457	258	153	5	124	364	140	385	8.80e-31	121
Msa0323630	AT3G59530.3	25.432	405	242	14	13	370	8	399	1.16e-30	121
Msa0323630	AT3G59530.2	25.432	405	242	14	13	370	8	399	1.16e-30	121
Msa0323630	AT3G59530.1	25.432	405	242	14	13	370	8	399	1.16e-30	121

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Find 96 sgRNAs with CRISPR-Local

Find 294 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATGAAGCAAAGAAGTGCT+TGG	0.534736	2_4:-27884608	Msa0323640:three_prime_UTR
AAATGGTGTTGCACTGTCCA+AGG	0.631186	2_4:+27882562	Msa0323630:CDS
AACTTCAGCTGTGGAGTTTG+AGG	0.394622	2_4:+27884750	Msa0323630:CDS
AATAACTGTCTCATTTAACG+TGG	0.678986	2_4:-27882516	Msa0323640:three_prime_UTR
AATATCAATCTTGCTCCTGA+TGG	0.526078	2_4:+27884053	Msa0323630:CDS
AATGGTTAGGAATGGAAATT+GGG	0.437047	2_4:+27880396	Msa0323630:CDS
AATGTGATTAAACTTGGAGA+AGG	0.561564	2_4:+27880292	Msa0323630:CDS
ACAAATTTGGTTTACACGAT+TGG	0.435403	2_4:+27882441	Msa0323630:CDS
ACAACCAGAAGATGTTTGTG+TGG	0.680429	2_4:+27880324	Msa0323630:CDS
ACCAAGGGTGGAAGGAGGAT+TGG	0.465023	2_4:-27880013	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GATGAACAAAATTCCTTTCT+TGG	+	chr2_4:27879790-27879809	Msa0323630:five_prime_UTR	30.0%
TCAAATAATTACGCCAAGAA+AGG	-	chr2_4:27879806-27879825	None:intergenic	30.0%
TGTGACACAATATTTACCAA+GGG	-	chr2_4:27880031-27880050	None:intergenic	30.0%
TCTAGTATAACTGGATCTAT+AGG	-	chr2_4:27880117-27880136	None:intergenic	30.0%
CAATTACAGGTGAAAACTTA+AGG	+	chr2_4:27880180-27880199	Msa0323630:intron	30.0%
TGACAGAATGTGATTAAACT+TGG	+	chr2_4:27880286-27880305	Msa0323630:intron	30.0%
AATGTGATTAAACTTGGAGA+AGG	+	chr2_4:27880292-27880311	Msa0323630:CDS	30.0%
AGATGGTTGGATTAAAAGAA+TGG	+	chr2_4:27880378-27880397	Msa0323630:CDS	30.0%
GTTGGATTAAAAGAATGGTT+AGG	+	chr2_4:27880383-27880402	Msa0323630:CDS	30.0%
AATGGTTAGGAATGGAAATT+GGG	+	chr2_4:27880396-27880415	Msa0323630:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_4	gene	27879782	27884987	27879782	ID=Msa0323630;Name=Msa0323630
chr2_4	mRNA	27879782	27884987	27879782	ID=Msa0323630-mRNA-1;Parent=Msa0323630;Name=Msa0323630-mRNA-1;_AED=0.28;_eAED=0.28;_QI=215\|1\|1\|1\|1\|1\|6\|150\|371
chr2_4	exon	27879782	27880188	27879782	ID=Msa0323630-mRNA-1:exon:9624;Parent=Msa0323630-mRNA-1
chr2_4	exon	27880292	27880504	27880292	ID=Msa0323630-mRNA-1:exon:9625;Parent=Msa0323630-mRNA-1
chr2_4	exon	27880624	27880691	27880624	ID=Msa0323630-mRNA-1:exon:9626;Parent=Msa0323630-mRNA-1
chr2_4	exon	27882382	27882618	27882382	ID=Msa0323630-mRNA-1:exon:9627;Parent=Msa0323630-mRNA-1
chr2_4	exon	27883968	27884100	27883968	ID=Msa0323630-mRNA-1:exon:9628;Parent=Msa0323630-mRNA-1
chr2_4	exon	27884565	27884987	27884565	ID=Msa0323630-mRNA-1:exon:9629;Parent=Msa0323630-mRNA-1
chr2_4	five_prime_UTR	27879782	27879996	27879782	ID=Msa0323630-mRNA-1:five_prime_utr;Parent=Msa0323630-mRNA-1
chr2_4	CDS	27879997	27880188	27879997	ID=Msa0323630-mRNA-1:cds;Parent=Msa0323630-mRNA-1
chr2_4	CDS	27880292	27880504	27880292	ID=Msa0323630-mRNA-1:cds;Parent=Msa0323630-mRNA-1
chr2_4	CDS	27880624	27880691	27880624	ID=Msa0323630-mRNA-1:cds;Parent=Msa0323630-mRNA-1
chr2_4	CDS	27882382	27882618	27882382	ID=Msa0323630-mRNA-1:cds;Parent=Msa0323630-mRNA-1
chr2_4	CDS	27883968	27884100	27883968	ID=Msa0323630-mRNA-1:cds;Parent=Msa0323630-mRNA-1
chr2_4	CDS	27884565	27884837	27884565	ID=Msa0323630-mRNA-1:cds;Parent=Msa0323630-mRNA-1
chr2_4	three_prime_UTR	27884838	27884987	27884838	ID=Msa0323630-mRNA-1:three_prime_utr;Parent=Msa0323630-mRNA-1

Gene Sequence

>Msa0323630

ATGGCTTCCAATATAACCAATCCTCCTTCCACCCTTGGTAAATATTGTGTCACACTGACCTCAAGCTTGGTTCTAGCAGCTTTAGTTGCTCTTACAGTTCAAGTCTTCTACTTCTCACCTATAGATCCAGTTATACTAGAAATTCCTTCTGCTGCATCTTCAACTTCTTCATTCAAAAACAACCAATTACAGAATGTGATTAAACTTGGAGAAGGATTTCTGAAACAACCAGAAGATGTTTGTGTGGATAAAGATGGTGTCCTATATACAGCTACAAGAGATGGTTGGATTAAAAGAATGGTTAGGAATGGAAATTGGGAGAATTGGAAGCATATAGATAGCTCTTCTTTGCTTGGAATCACTACATCTAAAGATGGTGGTCTCATTGTCTGTGATACTACCTTGGGTTTGCTCAAGGTTACAGAAGACGGTTTCAGTGTTATCCTTTCACAAGTGAATGGTTCTCAATTAATGTTTGCAGATGATGTAATAGAAGCATCAGATGGAAATATATACTTCAGTGTTGCTAGTAACAAATTTGGTTTACACGATTGGTATTTGGATTTGCTAGAGGCAAGACCTCATGGACAACTTTTAAAGTATAATCCCACGTTAAATGAGACAGTTATTGTCATTGATAATTTGACATTTGCAAATGGTGTTGCACTGTCCAAGGATGAAGATTATGTTGCGGTGTGCGAAACTTGGAAATTCAGGTGTGTGAGGCATTGGCTAAAAGGAATTAACAAAGGCAAAACAGATATCTTCATTGAAAACCTCCCTGGTGGACCTGACAATATCAATCTTGCTCCTGATGGCTCATTTTGGATCGCTTTGGTTCAATTAACTTCTAAAAGGCTGGGGTTTGTGCACACCTCTAAAGTATCCAAGCACTTCTTTGCTTCATTTCCAAGGCTATTTAATCTGATCAATAGTGCAACAAAATCAGCTATGGTGGTAAATGTTGGAACTGAAGGCAATATTATCAGAAAGTTTGGTGATAATGAAGGGAAGGTGATAAGTTTTGTAACTTCAGCTGTGGAGTTTGAGGACCATCTTTACTTAGGAAGTCTTAATTCTGACTTTGTTGGAAAATTGCCACTGTCAATTGCATAG

Protein sequence

>Msa0323630

MASNITNPPSTLGKYCVTLTSSLVLAALVALTVQVFYFSPIDPVILEIPSAASSTSSFKNNQLQNVIKLGEGFLKQPEDVCVDKDGVLYTATRDGWIKRMVRNGNWENWKHIDSSSLLGITTSKDGGLIVCDTTLGLLKVTEDGFSVILSQVNGSQLMFADDVIEASDGNIYFSVASNKFGLHDWYLDLLEARPHGQLLKYNPTLNETVIVIDNLTFANGVALSKDEDYVAVCETWKFRCVRHWLKGINKGKTDIFIENLPGGPDNINLAPDGSFWIALVQLTSKRLGFVHTSKVSKHFFASFPRLFNLINSATKSAMVVNVGTEGNIIRKFGDNEGKVISFVTSAVEFEDHLYLGSLNSDFVGKLPLSIA*