AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0418270

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0418270 MtrunA17_Chr3g0115881 100.000 74 0 0 1 74 1 74 2.59e-49 149
Msa0418270 MtrunA17_Chr7g0214091 89.130 46 5 0 1 46 1 46 4.54e-24 85.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0418270 AT1G27435.1 72.000 75 17 1 3 73 6 80 8.74e-33 108

Find 40 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATAGATGGGTGGTGCATTT+TGG 0.153987 3_2:-65932193 Msa0418270:intron
CTCAATCGCAGGAAGGGTTT+AGG 0.229490 3_2:+65932714 None:intergenic
AGGTTGTTCCATCGGTATTT+TGG 0.238148 3_2:-65931399 Msa0418270:three_prime_UTR
CACACGGCGAAGACGCTTCT+TGG 0.328655 3_2:+65932079 None:intergenic
GTTGACCGGCTTCATAAAAT+AGG 0.333484 3_2:+65931127 None:intergenic
TCAATCGCAGGAAGGGTTTA+GGG 0.333860 3_2:+65932715 None:intergenic
TGTAAGAAAGTTAAAATCAT+TGG 0.339565 3_2:+65931313 None:intergenic
CTCTCTGTTTCTCATCCCAC+AGG 0.373589 3_2:-65932637 Msa0418270:intron
AGTACTCTTTGTAACATTGT+TGG 0.406629 3_2:+65931445 None:intergenic
AGTGAAGAAGCATGATTCTT+CGG 0.422338 3_2:-65932150 Msa0418270:CDS
GCAGGAAGGGTTTAGGGTTG+AGG 0.423392 3_2:+65932721 None:intergenic
ATACCGATGGAACAACCTTC+AGG 0.424726 3_2:+65931404 None:intergenic
GCGAAGACGCTTCTTGGCAT+TGG 0.430692 3_2:+65932085 None:intergenic
ACTTGCTGAAGGAGCTGGTT+CGG 0.434113 3_2:+65931481 None:intergenic
GTGAAGAAGCATGATTCTTC+GGG 0.434713 3_2:-65932149 Msa0418270:CDS
GCGTCTTCGCCGTGTGTGGC+AGG 0.469382 3_2:-65932072 Msa0418270:intron
ACAAAGAGTACTTAAGTTGA+AGG 0.470492 3_2:-65931434 Msa0418270:exon
GGCATTGGCTTTACGAGTTG+TGG 0.490914 3_2:+65932100 None:intergenic
CTTTCTTCGTCTCTTCCCAC+AGG 0.499169 3_2:-65932670 Msa0418270:five_prime_UTR
CTTTGTAACATTGTTGGCAC+TGG 0.504174 3_2:+65931451 None:intergenic
GAAGAACTTGCTGAAGGAGC+TGG 0.514090 3_2:+65931476 None:intergenic
CACATTATAGAACTGTTGAC+CGG 0.525045 3_2:+65931113 None:intergenic
TTGGGGTACGCGGGTGATGG+AGG 0.527082 3_2:-65932174 Msa0418270:CDS
CCACCTGAAGGTTGTTCCAT+CGG 0.559409 3_2:-65931407 Msa0418270:three_prime_UTR
GAAGAATGAAGCTGTCCTCA+AGG 0.567341 3_2:-65931510 Msa0418270:intron
CAAAGAGTACTTAAGTTGAA+GGG 0.582974 3_2:-65931433 Msa0418270:exon
GTTGAAGGGTGTCCACCTGA+AGG 0.585228 3_2:-65931419 Msa0418270:three_prime_UTR
TGAATTGCTCAATCGCAGGA+AGG 0.599726 3_2:+65932707 None:intergenic
AGAAGCGTCTTCGCCGTGTG+TGG 0.603774 3_2:-65932076 Msa0418270:CDS
GAATTGCTCAATCGCAGGAA+GGG 0.604122 3_2:+65932708 None:intergenic
TTGGGATCCCAAAATACCGA+TGG 0.617975 3_2:+65931391 None:intergenic
CTGGCAGAAGAACTTGCTGA+AGG 0.618337 3_2:+65931470 None:intergenic
GAGAGAAGAAATAAACCTGT+GGG 0.623414 3_2:+65932655 None:intergenic
GGAAGGGTTTAGGGTTGAGG+AGG 0.633436 3_2:+65932724 None:intergenic
ATTGAAATACCTGCCACACA+CGG 0.634795 3_2:+65932063 None:intergenic
AGAGAGAAGAAATAAACCTG+TGG 0.644844 3_2:+65932654 None:intergenic
GATGTGAATTGCTCAATCGC+AGG 0.650179 3_2:+65932703 None:intergenic
CCGATGGAACAACCTTCAGG+TGG 0.677190 3_2:+65931407 None:intergenic
CTGGTTCGGAACAAGCCTTG+AGG 0.710113 3_2:+65931495 None:intergenic
GGTAAAATAAAATAGATGGG+TGG 0.724755 3_2:-65932203 Msa0418270:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAATAAAAAAGTAAATTAA+AGG + chr3_2:65931289-65931308 None:intergenic 10.0%
!! GAAATAAAAAAGTAAATTAA+AGG + chr3_2:65931289-65931308 None:intergenic 10.0%
!!! CATTTTTCAGATAAAATTAA+AGG + chr3_2:65931311-65931330 None:intergenic 15.0%
!! AAGGATGTTATTAATTAAAT+AGG - chr3_2:65931866-65931885 Msa0418270:intron 15.0%
!!! CATTTTTCAGATAAAATTAA+AGG + chr3_2:65931311-65931330 None:intergenic 15.0%
!! AAGGATGTTATTAATTAAAT+AGG - chr3_2:65931866-65931885 Msa0418270:intron 15.0%
!! TATACGTAGATAAATGATTA+AGG - chr3_2:65931847-65931866 Msa0418270:intron 20.0%
!!! AAACTATAAGTGAAACTTTT+AGG - chr3_2:65932089-65932108 Msa0418270:CDS 20.0%
!! CACAATTATTGTTCAATATT+TGG + chr3_2:65932286-65932305 None:intergenic 20.0%
!!! TATGGTACATTTTTTGTTTT+TGG - chr3_2:65932324-65932343 Msa0418270:intron 20.0%
!! GCAAAAACTAATATAGAATT+GGG + chr3_2:65932486-65932505 None:intergenic 20.0%
!! TGTAAGAAAGTTAAAATCAT+TGG + chr3_2:65932546-65932565 None:intergenic 20.0%
!!! TGTAAGTTGTAAACTTTTTT+AGG - chr3_2:65932576-65932595 Msa0418270:intron 20.0%
!!! GTAAGTTGTAAACTTTTTTA+GGG - chr3_2:65932577-65932596 Msa0418270:intron 20.0%
!! TATACGTAGATAAATGATTA+AGG - chr3_2:65931847-65931866 Msa0418270:intron 20.0%
!!! AAACTATAAGTGAAACTTTT+AGG - chr3_2:65932089-65932108 Msa0418270:CDS 20.0%
!! CACAATTATTGTTCAATATT+TGG + chr3_2:65932286-65932305 None:intergenic 20.0%
!!! TATGGTACATTTTTTGTTTT+TGG - chr3_2:65932324-65932343 Msa0418270:intron 20.0%
!! GCAAAAACTAATATAGAATT+GGG + chr3_2:65932486-65932505 None:intergenic 20.0%
!! TGTAAGAAAGTTAAAATCAT+TGG + chr3_2:65932546-65932565 None:intergenic 20.0%
!!! TGTAAGTTGTAAACTTTTTT+AGG - chr3_2:65932576-65932595 Msa0418270:intron 20.0%
!!! GTAAGTTGTAAACTTTTTTA+GGG - chr3_2:65932577-65932596 Msa0418270:intron 20.0%
! GATTGTGATATATGGTATTA+TGG - chr3_2:65931336-65931355 Msa0418270:three_prime_UTR 25.0%
!!! GAGTTTTAATATATGTGTAG+CGG - chr3_2:65931394-65931413 Msa0418270:three_prime_UTR 25.0%
!!! GTCTAAGTTTTTTACATTAG+CGG - chr3_2:65931422-65931441 Msa0418270:three_prime_UTR 25.0%
!!! TCTTCATAAATTTGTTACAC+TGG + chr3_2:65931509-65931528 None:intergenic 25.0%
!! TATGTATTTCTGATGAATGT+TGG - chr3_2:65931533-65931552 Msa0418270:intron 25.0%
! TGCTGGTAAAATAAAATAGA+TGG - chr3_2:65931649-65931668 Msa0418270:intron 25.0%
! GCTGGTAAAATAAAATAGAT+GGG - chr3_2:65931650-65931669 Msa0418270:intron 25.0%
! ATACCTAGAAGAAATGATTA+AGG - chr3_2:65931895-65931914 Msa0418270:intron 25.0%
!!! ATTTATTGTTTTATCGTCTG+TGG - chr3_2:65931924-65931943 Msa0418270:intron 25.0%
!! GTTTCACTTATAGTTTTTCT+AGG + chr3_2:65932085-65932104 None:intergenic 25.0%
!!! TGAAACTTTTAGGAAAAACT+TGG - chr3_2:65932099-65932118 Msa0418270:CDS 25.0%
! TATGGTTTGAGTAAAGATTA+TGG - chr3_2:65932144-65932163 Msa0418270:CDS 25.0%
! GGCAAAAACTAATATAGAAT+TGG + chr3_2:65932487-65932506 None:intergenic 25.0%
! GATTGTGATATATGGTATTA+TGG - chr3_2:65931336-65931355 Msa0418270:three_prime_UTR 25.0%
!!! GAGTTTTAATATATGTGTAG+CGG - chr3_2:65931394-65931413 Msa0418270:three_prime_UTR 25.0%
!!! GTCTAAGTTTTTTACATTAG+CGG - chr3_2:65931422-65931441 Msa0418270:three_prime_UTR 25.0%
!!! TCTTCATAAATTTGTTACAC+TGG + chr3_2:65931509-65931528 None:intergenic 25.0%
!! TATGTATTTCTGATGAATGT+TGG - chr3_2:65931533-65931552 Msa0418270:intron 25.0%
! TGCTGGTAAAATAAAATAGA+TGG - chr3_2:65931649-65931668 Msa0418270:intron 25.0%
! GCTGGTAAAATAAAATAGAT+GGG - chr3_2:65931650-65931669 Msa0418270:intron 25.0%
! ATACCTAGAAGAAATGATTA+AGG - chr3_2:65931895-65931914 Msa0418270:intron 25.0%
!!! ATTTATTGTTTTATCGTCTG+TGG - chr3_2:65931924-65931943 Msa0418270:intron 25.0%
!! GTTTCACTTATAGTTTTTCT+AGG + chr3_2:65932085-65932104 None:intergenic 25.0%
!!! TGAAACTTTTAGGAAAAACT+TGG - chr3_2:65932099-65932118 Msa0418270:CDS 25.0%
! TATGGTTTGAGTAAAGATTA+TGG - chr3_2:65932144-65932163 Msa0418270:CDS 25.0%
! GGCAAAAACTAATATAGAAT+TGG + chr3_2:65932487-65932506 None:intergenic 25.0%
ATGTTGCAGATTGTGATATA+TGG - chr3_2:65931328-65931347 Msa0418270:three_prime_UTR 30.0%
TGGTAATAATCTATGAAGCA+CGG + chr3_2:65931489-65931508 None:intergenic 30.0%
!!! TTGAATCTGATTTGTTTTGC+TGG - chr3_2:65931632-65931651 Msa0418270:intron 30.0%
GGTAAAATAAAATAGATGGG+TGG - chr3_2:65931653-65931672 Msa0418270:intron 30.0%
CATCCTTAATCATTTCTTCT+AGG + chr3_2:65931901-65931920 None:intergenic 30.0%
TGATGAGCATTTAGTAAAGA+TGG - chr3_2:65931953-65931972 Msa0418270:intron 30.0%
AAACTTGGAGATTAATGAGT+TGG - chr3_2:65932114-65932133 Msa0418270:CDS 30.0%
TCTTTACTCAAACCATATCT+TGG + chr3_2:65932141-65932160 None:intergenic 30.0%
ACAATAATTGTGACTGTCAA+TGG - chr3_2:65932294-65932313 Msa0418270:intron 30.0%
AGTACTCTTTGTAACATTGT+TGG + chr3_2:65932414-65932433 None:intergenic 30.0%
!! ACAAAGAGTACTTAAGTTGA+AGG - chr3_2:65932422-65932441 Msa0418270:intron 30.0%
!! CAAAGAGTACTTAAGTTGAA+GGG - chr3_2:65932423-65932442 Msa0418270:intron 30.0%
!! TCTACAGTTTTTCAAATGTG+TGG + chr3_2:65932508-65932527 None:intergenic 30.0%
ACACATTTGAAAAACTGTAG+AGG - chr3_2:65932507-65932526 Msa0418270:intron 30.0%
ATGTTGCAGATTGTGATATA+TGG - chr3_2:65931328-65931347 Msa0418270:three_prime_UTR 30.0%
TGGTAATAATCTATGAAGCA+CGG + chr3_2:65931489-65931508 None:intergenic 30.0%
!!! TTGAATCTGATTTGTTTTGC+TGG - chr3_2:65931632-65931651 Msa0418270:intron 30.0%
GGTAAAATAAAATAGATGGG+TGG - chr3_2:65931653-65931672 Msa0418270:intron 30.0%
CATCCTTAATCATTTCTTCT+AGG + chr3_2:65931901-65931920 None:intergenic 30.0%
TGATGAGCATTTAGTAAAGA+TGG - chr3_2:65931953-65931972 Msa0418270:intron 30.0%
AAACTTGGAGATTAATGAGT+TGG - chr3_2:65932114-65932133 Msa0418270:CDS 30.0%
TCTTTACTCAAACCATATCT+TGG + chr3_2:65932141-65932160 None:intergenic 30.0%
ACAATAATTGTGACTGTCAA+TGG - chr3_2:65932294-65932313 Msa0418270:intron 30.0%
AGTACTCTTTGTAACATTGT+TGG + chr3_2:65932414-65932433 None:intergenic 30.0%
!! ACAAAGAGTACTTAAGTTGA+AGG - chr3_2:65932422-65932441 Msa0418270:intron 30.0%
!! CAAAGAGTACTTAAGTTGAA+GGG - chr3_2:65932423-65932442 Msa0418270:intron 30.0%
!! TCTACAGTTTTTCAAATGTG+TGG + chr3_2:65932508-65932527 None:intergenic 30.0%
ACACATTTGAAAAACTGTAG+AGG - chr3_2:65932507-65932526 Msa0418270:intron 30.0%
GAGAGAAGAAATAAACCTGT+GGG + chr3_2:65931204-65931223 None:intergenic 35.0%
AGAGAGAAGAAATAAACCTG+TGG + chr3_2:65931205-65931224 None:intergenic 35.0%
GAGAGAAGAAATAAACCTGT+GGG + chr3_2:65931204-65931223 None:intergenic 35.0%
AGAGAGAAGAAATAAACCTG+TGG + chr3_2:65931205-65931224 None:intergenic 35.0%
GAAGCAATTTGTGATCAAAC+TGG + chr3_2:65931574-65931593 None:intergenic 35.0%
ACAAATTGCTTCTGACTACT+GGG - chr3_2:65931582-65931601 Msa0418270:intron 35.0%
! AGTGAAGAAGCATGATTCTT+CGG - chr3_2:65931706-65931725 Msa0418270:intron 35.0%
! ATGATTCTTCGGGACTTTTA+TGG - chr3_2:65931717-65931736 Msa0418270:intron 35.0%
TGAGCATTTAGTAAAGATGG+TGG - chr3_2:65931956-65931975 Msa0418270:intron 35.0%
!! AAGATGGTGGAAAATAAGCT+TGG - chr3_2:65931969-65931988 Msa0418270:intron 35.0%
GTGGAAAATAAGCTTGGTTT+GGG - chr3_2:65931975-65931994 Msa0418270:intron 35.0%
CTCCTAAGTACAAAATCTAC+AGG + chr3_2:65932016-65932035 None:intergenic 35.0%
! TAATGAGTTGGACCAAGATA+TGG - chr3_2:65932126-65932145 Msa0418270:CDS 35.0%
ACTGTCAATGGCTAATGTTA+TGG - chr3_2:65932306-65932325 Msa0418270:intron 35.0%
! TCTTGCCTATTTTATGAAGC+CGG - chr3_2:65932724-65932743 Msa0418270:five_prime_UTR 35.0%
GAGAGAAGAAATAAACCTGT+GGG + chr3_2:65931204-65931223 None:intergenic 35.0%
AGAGAGAAGAAATAAACCTG+TGG + chr3_2:65931205-65931224 None:intergenic 35.0%
GAGAGAAGAAATAAACCTGT+GGG + chr3_2:65931204-65931223 None:intergenic 35.0%
AGAGAGAAGAAATAAACCTG+TGG + chr3_2:65931205-65931224 None:intergenic 35.0%
GAAGCAATTTGTGATCAAAC+TGG + chr3_2:65931574-65931593 None:intergenic 35.0%
ACAAATTGCTTCTGACTACT+GGG - chr3_2:65931582-65931601 Msa0418270:intron 35.0%
! AGTGAAGAAGCATGATTCTT+CGG - chr3_2:65931706-65931725 Msa0418270:intron 35.0%
! ATGATTCTTCGGGACTTTTA+TGG - chr3_2:65931717-65931736 Msa0418270:intron 35.0%
TGAGCATTTAGTAAAGATGG+TGG - chr3_2:65931956-65931975 Msa0418270:intron 35.0%
!! AAGATGGTGGAAAATAAGCT+TGG - chr3_2:65931969-65931988 Msa0418270:intron 35.0%
GTGGAAAATAAGCTTGGTTT+GGG - chr3_2:65931975-65931994 Msa0418270:intron 35.0%
CTCCTAAGTACAAAATCTAC+AGG + chr3_2:65932016-65932035 None:intergenic 35.0%
! TAATGAGTTGGACCAAGATA+TGG - chr3_2:65932126-65932145 Msa0418270:CDS 35.0%
ACTGTCAATGGCTAATGTTA+TGG - chr3_2:65932306-65932325 Msa0418270:intron 35.0%
! TCTTGCCTATTTTATGAAGC+CGG - chr3_2:65932724-65932743 Msa0418270:five_prime_UTR 35.0%
!! TTTTTACATTAGCGGTGTCG+AGG - chr3_2:65931430-65931449 Msa0418270:exon 40.0%
GCAATTTGTGATCAAACTGG+CGG + chr3_2:65931571-65931590 None:intergenic 40.0%
CACAAATTGCTTCTGACTAC+TGG - chr3_2:65931581-65931600 Msa0418270:intron 40.0%
! AATAGATGGGTGGTGCATTT+TGG - chr3_2:65931663-65931682 Msa0418270:intron 40.0%
! ATAGATGGGTGGTGCATTTT+GGG - chr3_2:65931664-65931683 Msa0418270:intron 40.0%
! GTGAAGAAGCATGATTCTTC+GGG - chr3_2:65931707-65931726 Msa0418270:intron 40.0%
ATTGAAATACCTGCCACACA+CGG + chr3_2:65931796-65931815 None:intergenic 40.0%
GGTGGAAAATAAGCTTGGTT+TGG - chr3_2:65931974-65931993 Msa0418270:intron 40.0%
! GGCCTGTAGATTTTGTACTT+AGG - chr3_2:65932011-65932030 Msa0418270:intron 40.0%
ACTTAGGAGAGTAGATCAGA+TGG - chr3_2:65932027-65932046 Msa0418270:intron 40.0%
AGAGTAGATCAGATGGAGAA+TGG - chr3_2:65932034-65932053 Msa0418270:intron 40.0%
TAGATCAGATGGAGAATGGT+CGG - chr3_2:65932038-65932057 Msa0418270:intron 40.0%
CTTTGTAACATTGTTGGCAC+TGG + chr3_2:65932408-65932427 None:intergenic 40.0%
! AGGTTGTTCCATCGGTATTT+TGG - chr3_2:65932457-65932476 Msa0418270:intron 40.0%
! GGTTGTTCCATCGGTATTTT+GGG - chr3_2:65932458-65932477 Msa0418270:intron 40.0%
! GTTGACCGGCTTCATAAAAT+AGG + chr3_2:65932732-65932751 None:intergenic 40.0%
!! TTTTTACATTAGCGGTGTCG+AGG - chr3_2:65931430-65931449 Msa0418270:exon 40.0%
GCAATTTGTGATCAAACTGG+CGG + chr3_2:65931571-65931590 None:intergenic 40.0%
CACAAATTGCTTCTGACTAC+TGG - chr3_2:65931581-65931600 Msa0418270:intron 40.0%
! AATAGATGGGTGGTGCATTT+TGG - chr3_2:65931663-65931682 Msa0418270:intron 40.0%
! ATAGATGGGTGGTGCATTTT+GGG - chr3_2:65931664-65931683 Msa0418270:intron 40.0%
! GTGAAGAAGCATGATTCTTC+GGG - chr3_2:65931707-65931726 Msa0418270:intron 40.0%
ATTGAAATACCTGCCACACA+CGG + chr3_2:65931796-65931815 None:intergenic 40.0%
GGTGGAAAATAAGCTTGGTT+TGG - chr3_2:65931974-65931993 Msa0418270:intron 40.0%
! GGCCTGTAGATTTTGTACTT+AGG - chr3_2:65932011-65932030 Msa0418270:intron 40.0%
ACTTAGGAGAGTAGATCAGA+TGG - chr3_2:65932027-65932046 Msa0418270:intron 40.0%
AGAGTAGATCAGATGGAGAA+TGG - chr3_2:65932034-65932053 Msa0418270:intron 40.0%
TAGATCAGATGGAGAATGGT+CGG - chr3_2:65932038-65932057 Msa0418270:intron 40.0%
CTTTGTAACATTGTTGGCAC+TGG + chr3_2:65932408-65932427 None:intergenic 40.0%
! AGGTTGTTCCATCGGTATTT+TGG - chr3_2:65932457-65932476 Msa0418270:intron 40.0%
! GGTTGTTCCATCGGTATTTT+GGG - chr3_2:65932458-65932477 Msa0418270:intron 40.0%
! GTTGACCGGCTTCATAAAAT+AGG + chr3_2:65932732-65932751 None:intergenic 40.0%
TCAATCGCAGGAAGGGTTTA+GGG + chr3_2:65931144-65931163 None:intergenic 45.0%
GAATTGCTCAATCGCAGGAA+GGG + chr3_2:65931151-65931170 None:intergenic 45.0%
TGAATTGCTCAATCGCAGGA+AGG + chr3_2:65931152-65931171 None:intergenic 45.0%
GATGTGAATTGCTCAATCGC+AGG + chr3_2:65931156-65931175 None:intergenic 45.0%
! TAGATGGGTGGTGCATTTTG+GGG - chr3_2:65931665-65931684 Msa0418270:intron 45.0%
AAGCCCTATCTCACTAAGTG+GGG + chr3_2:65932193-65932212 None:intergenic 45.0%
CAAGCCCTATCTCACTAAGT+GGG + chr3_2:65932194-65932213 None:intergenic 45.0%
GAAGAATGAAGCTGTCCTCA+AGG - chr3_2:65932346-65932365 Msa0418270:intron 45.0%
ATACCGATGGAACAACCTTC+AGG + chr3_2:65932455-65932474 None:intergenic 45.0%
TTGGGATCCCAAAATACCGA+TGG + chr3_2:65932468-65932487 None:intergenic 45.0%
TCAATCGCAGGAAGGGTTTA+GGG + chr3_2:65931144-65931163 None:intergenic 45.0%
GAATTGCTCAATCGCAGGAA+GGG + chr3_2:65931151-65931170 None:intergenic 45.0%
TGAATTGCTCAATCGCAGGA+AGG + chr3_2:65931152-65931171 None:intergenic 45.0%
GATGTGAATTGCTCAATCGC+AGG + chr3_2:65931156-65931175 None:intergenic 45.0%
! TAGATGGGTGGTGCATTTTG+GGG - chr3_2:65931665-65931684 Msa0418270:intron 45.0%
AAGCCCTATCTCACTAAGTG+GGG + chr3_2:65932193-65932212 None:intergenic 45.0%
CAAGCCCTATCTCACTAAGT+GGG + chr3_2:65932194-65932213 None:intergenic 45.0%
GAAGAATGAAGCTGTCCTCA+AGG - chr3_2:65932346-65932365 Msa0418270:intron 45.0%
ATACCGATGGAACAACCTTC+AGG + chr3_2:65932455-65932474 None:intergenic 45.0%
TTGGGATCCCAAAATACCGA+TGG + chr3_2:65932468-65932487 None:intergenic 45.0%
CTCAATCGCAGGAAGGGTTT+AGG + chr3_2:65931145-65931164 None:intergenic 50.0%
CTTTCTTCGTCTCTTCCCAC+AGG - chr3_2:65931186-65931205 Msa0418270:three_prime_UTR 50.0%
! CTCTCTGTTTCTCATCCCAC+AGG - chr3_2:65931219-65931238 Msa0418270:three_prime_UTR 50.0%
ATGAAGCACGGACTCGAACA+CGG + chr3_2:65931477-65931496 None:intergenic 50.0%
!! GGCATTGGCTTTACGAGTTG+TGG + chr3_2:65931759-65931778 None:intergenic 50.0%
GGTTTGGGCATGTAGAGTGA+AGG - chr3_2:65931990-65932009 Msa0418270:intron 50.0%
GAGAATGGTCGGATCACTAG+AGG - chr3_2:65932049-65932068 Msa0418270:intron 50.0%
CCTATCTCACTAAGTGGGGT+CGG + chr3_2:65932189-65932208 None:intergenic 50.0%
CGACCCCACTTAGTGAGATA+GGG - chr3_2:65932187-65932206 Msa0418270:CDS 50.0%
CCAAGCCCTATCTCACTAAG+TGG + chr3_2:65932195-65932214 None:intergenic 50.0%
CCACTTAGTGAGATAGGGCT+TGG - chr3_2:65932192-65932211 Msa0418270:intron 50.0%
! ACTTGCTGAAGGAGCTGGTT+CGG + chr3_2:65932378-65932397 None:intergenic 50.0%
!! GAAGAACTTGCTGAAGGAGC+TGG + chr3_2:65932383-65932402 None:intergenic 50.0%
!! CTGGCAGAAGAACTTGCTGA+AGG + chr3_2:65932389-65932408 None:intergenic 50.0%
CCACCTGAAGGTTGTTCCAT+CGG - chr3_2:65932449-65932468 Msa0418270:intron 50.0%
CTCAATCGCAGGAAGGGTTT+AGG + chr3_2:65931145-65931164 None:intergenic 50.0%
CTTTCTTCGTCTCTTCCCAC+AGG - chr3_2:65931186-65931205 Msa0418270:three_prime_UTR 50.0%
! CTCTCTGTTTCTCATCCCAC+AGG - chr3_2:65931219-65931238 Msa0418270:three_prime_UTR 50.0%
ATGAAGCACGGACTCGAACA+CGG + chr3_2:65931477-65931496 None:intergenic 50.0%
!! GGCATTGGCTTTACGAGTTG+TGG + chr3_2:65931759-65931778 None:intergenic 50.0%
GGTTTGGGCATGTAGAGTGA+AGG - chr3_2:65931990-65932009 Msa0418270:intron 50.0%
GAGAATGGTCGGATCACTAG+AGG - chr3_2:65932049-65932068 Msa0418270:intron 50.0%
CCTATCTCACTAAGTGGGGT+CGG + chr3_2:65932189-65932208 None:intergenic 50.0%
CGACCCCACTTAGTGAGATA+GGG - chr3_2:65932187-65932206 Msa0418270:CDS 50.0%
CCAAGCCCTATCTCACTAAG+TGG + chr3_2:65932195-65932214 None:intergenic 50.0%
CCACTTAGTGAGATAGGGCT+TGG - chr3_2:65932192-65932211 Msa0418270:intron 50.0%
! ACTTGCTGAAGGAGCTGGTT+CGG + chr3_2:65932378-65932397 None:intergenic 50.0%
!! GAAGAACTTGCTGAAGGAGC+TGG + chr3_2:65932383-65932402 None:intergenic 50.0%
!! CTGGCAGAAGAACTTGCTGA+AGG + chr3_2:65932389-65932408 None:intergenic 50.0%
CCACCTGAAGGTTGTTCCAT+CGG - chr3_2:65932449-65932468 Msa0418270:intron 50.0%
! GGAAGGGTTTAGGGTTGAGG+AGG + chr3_2:65931135-65931154 None:intergenic 55.0%
! GCAGGAAGGGTTTAGGGTTG+AGG + chr3_2:65931138-65931157 None:intergenic 55.0%
!!! GTGGTGCATTTTGGGGTACG+CGG - chr3_2:65931672-65931691 Msa0418270:intron 55.0%
!!! TGGTGCATTTTGGGGTACGC+GGG - chr3_2:65931673-65931692 Msa0418270:intron 55.0%
!!! ATTTTGGGGTACGCGGGTGA+TGG - chr3_2:65931679-65931698 Msa0418270:intron 55.0%
GCGAAGACGCTTCTTGGCAT+TGG + chr3_2:65931774-65931793 None:intergenic 55.0%
GGTCGGATCACTAGAGGAAG+AGG - chr3_2:65932055-65932074 Msa0418270:intron 55.0%
ACTAAGTGGGGTCGGCTACA+TGG + chr3_2:65932181-65932200 None:intergenic 55.0%
CCGACCCCACTTAGTGAGAT+AGG - chr3_2:65932186-65932205 Msa0418270:CDS 55.0%
!! CTGGTTCGGAACAAGCCTTG+AGG + chr3_2:65932364-65932383 None:intergenic 55.0%
!! GTTGAAGGGTGTCCACCTGA+AGG - chr3_2:65932437-65932456 Msa0418270:intron 55.0%
CCGATGGAACAACCTTCAGG+TGG + chr3_2:65932452-65932471 None:intergenic 55.0%
! GGAAGGGTTTAGGGTTGAGG+AGG + chr3_2:65931135-65931154 None:intergenic 55.0%
! GCAGGAAGGGTTTAGGGTTG+AGG + chr3_2:65931138-65931157 None:intergenic 55.0%
!!! GTGGTGCATTTTGGGGTACG+CGG - chr3_2:65931672-65931691 Msa0418270:intron 55.0%
!!! TGGTGCATTTTGGGGTACGC+GGG - chr3_2:65931673-65931692 Msa0418270:intron 55.0%
!!! ATTTTGGGGTACGCGGGTGA+TGG - chr3_2:65931679-65931698 Msa0418270:intron 55.0%
GCGAAGACGCTTCTTGGCAT+TGG + chr3_2:65931774-65931793 None:intergenic 55.0%
GGTCGGATCACTAGAGGAAG+AGG - chr3_2:65932055-65932074 Msa0418270:intron 55.0%
ACTAAGTGGGGTCGGCTACA+TGG + chr3_2:65932181-65932200 None:intergenic 55.0%
CCGACCCCACTTAGTGAGAT+AGG - chr3_2:65932186-65932205 Msa0418270:CDS 55.0%
!! CTGGTTCGGAACAAGCCTTG+AGG + chr3_2:65932364-65932383 None:intergenic 55.0%
!! GTTGAAGGGTGTCCACCTGA+AGG - chr3_2:65932437-65932456 Msa0418270:intron 55.0%
CCGATGGAACAACCTTCAGG+TGG + chr3_2:65932452-65932471 None:intergenic 55.0%
! CGAGGTGTCTGTGTCGTGTC+AGG - chr3_2:65931448-65931467 Msa0418270:CDS 60.0%
CACACGGCGAAGACGCTTCT+TGG + chr3_2:65931780-65931799 None:intergenic 60.0%
!! AGAAGCGTCTTCGCCGTGTG+TGG - chr3_2:65931780-65931799 Msa0418270:intron 60.0%
! CGAGGTGTCTGTGTCGTGTC+AGG - chr3_2:65931448-65931467 Msa0418270:CDS 60.0%
CACACGGCGAAGACGCTTCT+TGG + chr3_2:65931780-65931799 None:intergenic 60.0%
!! AGAAGCGTCTTCGCCGTGTG+TGG - chr3_2:65931780-65931799 Msa0418270:intron 60.0%
! TTGGGGTACGCGGGTGATGG+AGG - chr3_2:65931682-65931701 Msa0418270:intron 65.0%
! TTGGGGTACGCGGGTGATGG+AGG - chr3_2:65931682-65931701 Msa0418270:intron 65.0%
GCGTCTTCGCCGTGTGTGGC+AGG - chr3_2:65931784-65931803 Msa0418270:intron 70.0%
GCGTCTTCGCCGTGTGTGGC+AGG - chr3_2:65931784-65931803 Msa0418270:intron 70.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 65931115 65932763 65931115 ID=Msa0418270;Name=Msa0418270
chr3_2 mRNA 65931115 65932763 65931115 ID=Msa0418270-mRNA-1;Parent=Msa0418270;Name=Msa0418270-mRNA-1;_AED=0.01;_eAED=0.01;_QI=126|1|1|1|0.5|0.66|3|327|74
chr3_2 exon 65931115 65931528 65931115 ID=Msa0418270-mRNA-1:exon:14920;Parent=Msa0418270-mRNA-1
chr3_2 exon 65932073 65932210 65932073 ID=Msa0418270-mRNA-1:exon:14919;Parent=Msa0418270-mRNA-1
chr3_2 exon 65932638 65932763 65932638 ID=Msa0418270-mRNA-1:exon:14918;Parent=Msa0418270-mRNA-1
chr3_2 five_prime_UTR 65932638 65932763 65932638 ID=Msa0418270-mRNA-1:five_prime_utr;Parent=Msa0418270-mRNA-1
chr3_2 CDS 65932073 65932210 65932073 ID=Msa0418270-mRNA-1:cds;Parent=Msa0418270-mRNA-1
chr3_2 CDS 65931442 65931528 65931442 ID=Msa0418270-mRNA-1:cds;Parent=Msa0418270-mRNA-1
chr3_2 three_prime_UTR 65931115 65931441 65931115 ID=Msa0418270-mRNA-1:three_prime_utr;Parent=Msa0418270-mRNA-1
Gene Sequence

>Msa0418270

ATGGGTGGTGCATTTTGGGGTACGCGGGTGATGGAGGTAGTGAAGAAGCATGATTCTTCGGGACTTTTATGGAAGCGAATCAAACTCACCACAACTCGTAAAGCCAATGCCAAGAAGCGTCTTCGCCGTGTGTGGCAGAATGAAGCTGTCCTCAAGGCTTGTTCCGAACCAGCTCCTTCAGCAAGTTCTTCTGCCAGTGCCAACAATGTTACAAAGAGTACTTAA
Protein sequence

>Msa0418270

MGGAFWGTRVMEVVKKHDSSGLLWKRIKLTTTRKANAKKRLRRVWQNEAVLKACSEPAPSASSSASANNVTKST*