Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0230750 | KEH37521.1 | 92.571 | 175 | 9 | 3 | 1 | 172 | 1 | 174 | 1.06e-110 | 323 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0230750 | sp|P51614|CHIT3_VITVI | 43.605 | 172 | 91 | 4 | 1 | 168 | 1 | 170 | 4.77e-39 | 137 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0230750 | A0A072V7B3 | 92.571 | 175 | 9 | 3 | 1 | 172 | 1 | 174 | 5.05e-111 | 323 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0000190 | Msa0230750 | 0.829535 | 4.876729e-55 | -8.615850e-47 |
| Msa0005890 | Msa0230750 | 0.814777 | 1.296300e-51 | -8.615850e-47 |
| Msa0017510 | Msa0230750 | 0.819672 | 1.027024e-52 | -8.615850e-47 |
| Msa0024550 | Msa0230750 | 0.824642 | 7.226030e-54 | -8.615850e-47 |
| Msa0028420 | Msa0230750 | 0.812372 | 4.381802e-51 | -8.615850e-47 |
| Msa0042610 | Msa0230750 | 0.801541 | 8.576450e-49 | -8.615850e-47 |
| Msa0074240 | Msa0230750 | 0.810090 | 1.370266e-50 | -8.615850e-47 |
| Msa0076250 | Msa0230750 | 0.808753 | 2.652743e-50 | -8.615850e-47 |
| Msa0089630 | Msa0230750 | 0.824104 | 9.668574e-54 | -8.615850e-47 |
| Msa0120400 | Msa0230750 | 0.820409 | 6.963372e-53 | -8.615850e-47 |
| Msa0157290 | Msa0230750 | 0.820384 | 7.057949e-53 | -8.615850e-47 |
| Msa0164330 | Msa0230750 | 0.809353 | 1.972964e-50 | -8.615850e-47 |
| Msa0168500 | Msa0230750 | 0.809043 | 2.299047e-50 | -8.615850e-47 |
| Msa0168650 | Msa0230750 | 0.803272 | 3.772997e-49 | -8.615850e-47 |
| Msa0178560 | Msa0230750 | 0.818484 | 1.913270e-52 | -8.615850e-47 |
| Msa0223080 | Msa0230750 | 0.807981 | 3.874929e-50 | -8.615850e-47 |
| Msa0225860 | Msa0230750 | 0.801075 | 1.068283e-48 | -8.615850e-47 |
| Msa0230740 | Msa0230750 | 0.925654 | 1.368025e-90 | -8.615850e-47 |
| Msa0230750 | Msa0261340 | 0.811487 | 6.832326e-51 | -8.615850e-47 |
| Msa0230750 | Msa0287740 | 0.802979 | 4.338540e-49 | -8.615850e-47 |
| Msa0230750 | Msa0302160 | 0.806090 | 9.740615e-50 | -8.615850e-47 |
| Msa0230750 | Msa0302170 | 0.802207 | 6.259388e-49 | -8.615850e-47 |
| Msa0230750 | Msa0339490 | 0.806994 | 6.276882e-50 | -8.615850e-47 |
| Msa0230750 | Msa0371030 | 0.802089 | 6.619569e-49 | -8.615850e-47 |
| Msa0230750 | Msa0378720 | 0.809541 | 1.798471e-50 | -8.615850e-47 |
| Msa0230750 | Msa0385390 | 0.812571 | 3.965989e-51 | -8.615850e-47 |
| Msa0230750 | Msa0392420 | 0.824566 | 7.528690e-54 | -8.615850e-47 |
| Msa0230750 | Msa0392460 | 0.813639 | 2.311927e-51 | -8.615850e-47 |
| Msa0230750 | Msa0392490 | 0.815174 | 1.058356e-51 | -8.615850e-47 |
| Msa0230750 | Msa0415320 | 0.839146 | 1.886651e-57 | -8.615850e-47 |
| Msa0230750 | Msa0428370 | 0.803909 | 2.783550e-49 | -8.615850e-47 |
| Msa0230750 | Msa0474730 | 0.801781 | 7.658184e-49 | -8.615850e-47 |
| Msa0230750 | Msa0487570 | 0.823665 | 1.225499e-53 | -8.615850e-47 |
| Msa0230750 | Msa0487630 | 0.825981 | 3.484289e-54 | -8.615850e-47 |
| Msa0230750 | Msa0523000 | 0.832959 | 7.019092e-56 | -8.615850e-47 |
| Msa0230750 | Msa0534930 | 0.818944 | 1.504832e-52 | -8.615850e-47 |
| Msa0230750 | Msa0535040 | 0.846481 | 2.114169e-59 | -8.615850e-47 |
| Msa0230750 | Msa0546500 | 0.801056 | 1.077760e-48 | -8.615850e-47 |
| Msa0230750 | Msa0580370 | 0.800711 | 1.267925e-48 | -8.615850e-47 |
| Msa0230750 | Msa0591790 | 0.816186 | 6.294739e-52 | -8.615850e-47 |
| Msa0230750 | Msa0626510 | 0.807285 | 5.446277e-50 | -8.615850e-47 |
| Msa0230750 | Msa0643080 | 0.803076 | 4.142608e-49 | -8.615850e-47 |
| Msa0230750 | Msa0739730 | 0.826630 | 2.441418e-54 | -8.615850e-47 |
| Msa0230750 | Msa0769180 | 0.815265 | 1.010315e-51 | -8.615850e-47 |
| Msa0230750 | Msa0785250 | 0.813071 | 3.081938e-51 | -8.615850e-47 |
| Msa0230750 | Msa0880140 | 0.817062 | 4.006622e-52 | -8.615850e-47 |
| Msa0230750 | Msa0896540 | 0.814466 | 1.518707e-51 | -8.615850e-47 |
| Msa0230750 | Msa0925180 | 0.831257 | 1.849879e-55 | -8.615850e-47 |
| Msa0230750 | Msa0967380 | 0.817403 | 3.358491e-52 | -8.615850e-47 |
| Msa0230750 | Msa0967870 | 0.801720 | 7.882329e-49 | -8.615850e-47 |
| Msa0230750 | Msa0990540 | 0.818304 | 2.102166e-52 | -8.615850e-47 |
| Msa0230750 | Msa1019430 | 0.826119 | 3.231322e-54 | -8.615850e-47 |
| Msa0230750 | Msa1019470 | 0.824425 | 8.127873e-54 | -8.615850e-47 |
| Msa0230750 | Msa1045140 | 0.800896 | 1.162421e-48 | -8.615850e-47 |
| Msa0230750 | Msa1078170 | 0.802281 | 6.043890e-49 | -8.615850e-47 |
| Msa0230750 | Msa1123380 | 0.809186 | 2.143067e-50 | -8.615850e-47 |
| Msa0230750 | Msa1140210 | 0.802384 | 5.756531e-49 | -8.615850e-47 |
| Msa0230750 | Msa1209460 | 0.802985 | 4.325763e-49 | -8.615850e-47 |
| Msa0230750 | Msa1228190 | 0.818658 | 1.747095e-52 | -8.615850e-47 |
| Msa0230750 | Msa1326630 | 0.800759 | 1.239479e-48 | -8.615850e-47 |
| Msa0230750 | Msa1345420 | 0.800330 | 1.516122e-48 | -8.615850e-47 |
| Msa0230750 | Msa1366340 | 0.813447 | 2.548152e-51 | -8.615850e-47 |
| Msa0230750 | Msa1372230 | 0.830335 | 3.112542e-55 | -8.615850e-47 |
| Msa0230750 | Msa1374550 | 0.817498 | 3.196216e-52 | -8.615850e-47 |
| Msa0230750 | Msa1438250 | 0.810955 | 8.908803e-51 | -8.615850e-47 |
| Msa0230750 | Msa1446820 | 0.804058 | 2.591426e-49 | -8.615850e-47 |
| Msa0230750 | Msa1451160 | 0.800604 | 1.333198e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0230750 | MtrunA17_Chr2g0300181 | 92.571 | 175 | 9 | 3 | 1 | 172 | 1 | 174 | 1.87e-112 | 323 |
| Msa0230750 | MtrunA17_Chr2g0300141 | 65.318 | 173 | 58 | 2 | 1 | 172 | 1 | 172 | 5.18e-72 | 220 |
| Msa0230750 | MtrunA17_Chr2g0300081 | 65.143 | 175 | 57 | 3 | 1 | 172 | 1 | 174 | 7.13e-69 | 212 |
| Msa0230750 | MtrunA17_Chr2g0300101 | 65.294 | 170 | 57 | 2 | 1 | 169 | 1 | 169 | 8.21e-69 | 211 |
| Msa0230750 | MtrunA17_Chr2g0300161 | 61.850 | 173 | 65 | 1 | 1 | 172 | 1 | 173 | 5.64e-67 | 202 |
| Msa0230750 | MtrunA17_Chr2g0300151 | 57.792 | 154 | 55 | 3 | 1 | 146 | 1 | 152 | 1.23e-55 | 172 |
| Msa0230750 | MtrunA17_Chr2g0300051 | 59.064 | 171 | 63 | 4 | 1 | 169 | 1 | 166 | 5.45e-55 | 176 |
| Msa0230750 | MtrunA17_Chr7g0275231 | 45.860 | 157 | 81 | 2 | 1 | 153 | 1 | 157 | 2.74e-42 | 143 |
| Msa0230750 | MtrunA17_Chr7g0240891 | 45.509 | 167 | 84 | 4 | 5 | 164 | 2 | 168 | 7.92e-42 | 141 |
| Msa0230750 | MtrunA17_Chr5g0418461 | 51.034 | 145 | 67 | 3 | 24 | 164 | 21 | 165 | 4.37e-41 | 139 |
| Msa0230750 | MtrunA17_Chr1g0202261 | 47.260 | 146 | 72 | 3 | 24 | 164 | 21 | 166 | 1.97e-39 | 135 |
| Msa0230750 | MtrunA17_Chr1g0202241 | 43.590 | 156 | 85 | 2 | 12 | 164 | 10 | 165 | 1.05e-38 | 133 |
| Msa0230750 | MtrunA17_Chr1g0202281 | 45.517 | 145 | 75 | 3 | 24 | 164 | 38 | 182 | 1.57e-37 | 130 |
| Msa0230750 | MtrunA17_Chr1g0202231 | 43.452 | 168 | 90 | 4 | 1 | 164 | 1 | 167 | 9.26e-35 | 124 |
| Msa0230750 | MtrunA17_Chr8g0361941 | 42.138 | 159 | 86 | 3 | 12 | 164 | 10 | 168 | 3.59e-34 | 122 |
| Msa0230750 | MtrunA17_Chr2g0331781 | 41.765 | 170 | 86 | 6 | 7 | 164 | 13 | 181 | 5.57e-32 | 117 |
| Msa0230750 | MtrunA17_Chr6g0459141 | 49.541 | 109 | 53 | 2 | 24 | 131 | 23 | 130 | 2.17e-31 | 113 |
| Msa0230750 | MtrunA17_Chr7g0274861 | 39.375 | 160 | 72 | 3 | 8 | 164 | 6 | 143 | 1.00e-30 | 112 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0230750 | AT5G24090.1 | 40.854 | 164 | 93 | 3 | 5 | 164 | 7 | 170 | 1.35e-36 | 128 |
Find 38 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTATTTACTAACTTCCTTAA+TGG | 0.218965 | 2_2:-2943251 | Msa0230750:CDS |
| AACTTCCTTAATGGTCAATT+TGG | 0.229685 | 2_2:-2943242 | Msa0230750:CDS |
| TGTTGACAATGAATTATTTC+GGG | 0.245856 | 2_2:+2943365 | None:intergenic |
| TACTTTGAATGGAATTAGTT+TGG | 0.267005 | 2_2:-2943204 | Msa0230750:CDS |
| TTGTTGACAATGAATTATTT+CGG | 0.275674 | 2_2:+2943364 | None:intergenic |
| TGGCAATATGGTTGTCTATT+GGG | 0.292929 | 2_2:-2943546 | Msa0230750:CDS |
| CTTGGAAGTGTTACTTTGAA+TGG | 0.306397 | 2_2:-2943215 | Msa0230750:CDS |
| ATCATCAAATGGTGGCAATA+TGG | 0.331767 | 2_2:-2943558 | Msa0230750:CDS |
| ATAATTCATTGTCAACAAAA+AGG | 0.352905 | 2_2:-2943359 | Msa0230750:CDS |
| GACCTATGAAGTTTAACTTT+GGG | 0.360131 | 2_2:+2943423 | None:intergenic |
| TCTACATACGACAAAATTAC+AGG | 0.369572 | 2_2:-2943133 | Msa0230750:CDS |
| GTGGCAATATGGTTGTCTAT+TGG | 0.373642 | 2_2:-2943547 | Msa0230750:CDS |
| ACCGCAATATGGTTTCAAAA+AGG | 0.375145 | 2_2:-2943634 | None:intergenic |
| GTGGACCAAATTGACCATTA+AGG | 0.383641 | 2_2:+2943237 | None:intergenic |
| TGACCTATGAAGTTTAACTT+TGG | 0.403106 | 2_2:+2943422 | None:intergenic |
| TTCCTTCTTGAAAGATATTG+AGG | 0.411809 | 2_2:+2943450 | None:intergenic |
| GGAATTAGTTTGGATATTCA+AGG | 0.439079 | 2_2:-2943194 | Msa0230750:CDS |
| TTCAAGGAGGTCCAGATCAA+TGG | 0.449888 | 2_2:-2943178 | Msa0230750:CDS |
| GGCAATATGGTTGTCTATTG+GGG | 0.450971 | 2_2:-2943545 | Msa0230750:CDS |
| GCAAAGAACTCCCATTGATC+TGG | 0.465987 | 2_2:+2943167 | None:intergenic |
| TCAAGGAGGTCCAGATCAAT+GGG | 0.485953 | 2_2:-2943177 | Msa0230750:CDS |
| GACTATGAAACCTACTCTCT+TGG | 0.486009 | 2_2:-2943308 | Msa0230750:CDS |
| TTGAAATCAACTTGTGACAC+TGG | 0.504021 | 2_2:-2943500 | Msa0230750:CDS |
| ATATTGCCACCATTTGATGA+TGG | 0.509465 | 2_2:+2943560 | None:intergenic |
| ACTATCAAACCATCATCAAA+TGG | 0.510231 | 2_2:-2943569 | Msa0230750:CDS |
| TTCCTCAATATCTTTCAAGA+AGG | 0.510674 | 2_2:-2943452 | Msa0230750:CDS |
| ATATTGTTAAGACCAAGAAA+AGG | 0.524452 | 2_2:+2943600 | None:intergenic |
| AATGGTCAATTTGGTCCACT+TGG | 0.524588 | 2_2:-2943233 | Msa0230750:CDS |
| ATCCCAAAGTTAAACTTCAT+AGG | 0.538270 | 2_2:-2943425 | Msa0230750:CDS |
| TATTGTTAAGACCAAGAAAA+GGG | 0.544637 | 2_2:+2943601 | None:intergenic |
| GGTTTCATAGTCACCTCCAA+TGG | 0.548013 | 2_2:+2943319 | None:intergenic |
| TTCTATTGAACCAAGAGAGT+AGG | 0.571812 | 2_2:+2943298 | None:intergenic |
| TGATGATGGTTTGATAGTGA+AGG | 0.576846 | 2_2:+2943574 | None:intergenic |
| GATGATGGTTTGATAGTGAA+GGG | 0.600294 | 2_2:+2943575 | None:intergenic |
| ATCAAACCATCATCAAATGG+TGG | 0.640431 | 2_2:-2943566 | Msa0230750:CDS |
| TTCAAAGTAACACTTCCAAG+TGG | 0.663392 | 2_2:+2943218 | None:intergenic |
| ATGATGGTTTGATAGTGAAG+GGG | 0.664735 | 2_2:+2943576 | None:intergenic |
| ATTAGTTTGGATATTCAAGG+AGG | 0.668285 | 2_2:-2943191 | Msa0230750:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTGTTGACAATGAATTATTT+CGG | + | chr2_2:2943395-2943414 | None:intergenic | 20.0% |
| !! | ATAATTCATTGTCAACAAAA+AGG | - | chr2_2:2943397-2943416 | Msa0230750:CDS | 20.0% |
| ! | TATTGTTAAGACCAAGAAAA+GGG | + | chr2_2:2943158-2943177 | None:intergenic | 25.0% |
| ! | ATATTGTTAAGACCAAGAAA+AGG | + | chr2_2:2943159-2943178 | None:intergenic | 25.0% |
| ! | TGTTGACAATGAATTATTTC+GGG | + | chr2_2:2943394-2943413 | None:intergenic | 25.0% |
| ! | CTATTTACTAACTTCCTTAA+TGG | - | chr2_2:2943505-2943524 | Msa0230750:CDS | 25.0% |
| ! | TACTTTGAATGGAATTAGTT+TGG | - | chr2_2:2943552-2943571 | Msa0230750:CDS | 25.0% |
| ACTATCAAACCATCATCAAA+TGG | - | chr2_2:2943187-2943206 | Msa0230750:CDS | 30.0% | |
| TTCCTCAATATCTTTCAAGA+AGG | - | chr2_2:2943304-2943323 | Msa0230750:CDS | 30.0% | |
| TTCCTTCTTGAAAGATATTG+AGG | + | chr2_2:2943309-2943328 | None:intergenic | 30.0% | |
| ATCCCAAAGTTAAACTTCAT+AGG | - | chr2_2:2943331-2943350 | Msa0230750:CDS | 30.0% | |
| ! | GACCTATGAAGTTTAACTTT+GGG | + | chr2_2:2943336-2943355 | None:intergenic | 30.0% |
| ! | TGACCTATGAAGTTTAACTT+TGG | + | chr2_2:2943337-2943356 | None:intergenic | 30.0% |
| ! | GTGAAAGTTTTTCTTTCCAT+TGG | - | chr2_2:2943421-2943440 | Msa0230750:CDS | 30.0% |
| ! | AAAGTTTTTCTTTCCATTGG+AGG | - | chr2_2:2943424-2943443 | Msa0230750:CDS | 30.0% |
| AACTTCCTTAATGGTCAATT+TGG | - | chr2_2:2943514-2943533 | Msa0230750:CDS | 30.0% | |
| ! | GGAATTAGTTTGGATATTCA+AGG | - | chr2_2:2943562-2943581 | Msa0230750:CDS | 30.0% |
| ! | ATTAGTTTGGATATTCAAGG+AGG | - | chr2_2:2943565-2943584 | Msa0230750:CDS | 30.0% |
| TCTACATACGACAAAATTAC+AGG | - | chr2_2:2943623-2943642 | Msa0230750:CDS | 30.0% | |
| !! | ATGATGGTTTGATAGTGAAG+GGG | + | chr2_2:2943183-2943202 | None:intergenic | 35.0% |
| !! | GATGATGGTTTGATAGTGAA+GGG | + | chr2_2:2943184-2943203 | None:intergenic | 35.0% |
| !! | TGATGATGGTTTGATAGTGA+AGG | + | chr2_2:2943185-2943204 | None:intergenic | 35.0% |
| ATCAAACCATCATCAAATGG+TGG | - | chr2_2:2943190-2943209 | Msa0230750:CDS | 35.0% | |
| ATATTGCCACCATTTGATGA+TGG | + | chr2_2:2943199-2943218 | None:intergenic | 35.0% | |
| ATCATCAAATGGTGGCAATA+TGG | - | chr2_2:2943198-2943217 | Msa0230750:CDS | 35.0% | |
| TGGCAATATGGTTGTCTATT+GGG | - | chr2_2:2943210-2943229 | Msa0230750:CDS | 35.0% | |
| TTGAAATCAACTTGTGACAC+TGG | - | chr2_2:2943256-2943275 | Msa0230750:CDS | 35.0% | |
| TTCTATTGAACCAAGAGAGT+AGG | + | chr2_2:2943461-2943480 | None:intergenic | 35.0% | |
| TTCAAAGTAACACTTCCAAG+TGG | + | chr2_2:2943541-2943560 | None:intergenic | 35.0% | |
| !! | CTTGGAAGTGTTACTTTGAA+TGG | - | chr2_2:2943541-2943560 | Msa0230750:CDS | 35.0% |
| GTGGCAATATGGTTGTCTAT+TGG | - | chr2_2:2943209-2943228 | Msa0230750:CDS | 40.0% | |
| GGCAATATGGTTGTCTATTG+GGG | - | chr2_2:2943211-2943230 | Msa0230750:CDS | 40.0% | |
| GACTATGAAACCTACTCTCT+TGG | - | chr2_2:2943448-2943467 | Msa0230750:CDS | 40.0% | |
| GTGGACCAAATTGACCATTA+AGG | + | chr2_2:2943522-2943541 | None:intergenic | 40.0% | |
| AATGGTCAATTTGGTCCACT+TGG | - | chr2_2:2943523-2943542 | Msa0230750:CDS | 40.0% | |
| !!! | GCAAGCATTGACCCTTTTCT+TGG | - | chr2_2:2943144-2943163 | Msa0230750:CDS | 45.0% |
| !! | CGGGTTCAAGTTTTGTGCAA+GGG | + | chr2_2:2943375-2943394 | None:intergenic | 45.0% |
| !! | TCGGGTTCAAGTTTTGTGCA+AGG | + | chr2_2:2943376-2943395 | None:intergenic | 45.0% |
| GGTTTCATAGTCACCTCCAA+TGG | + | chr2_2:2943440-2943459 | None:intergenic | 45.0% | |
| TTCAAGGAGGTCCAGATCAA+TGG | - | chr2_2:2943578-2943597 | Msa0230750:CDS | 45.0% | |
| TCAAGGAGGTCCAGATCAAT+GGG | - | chr2_2:2943579-2943598 | Msa0230750:CDS | 45.0% | |
| GCAAAGAACTCCCATTGATC+TGG | + | chr2_2:2943592-2943611 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_2 | gene | 2943130 | 2943648 | 2943130 | ID=Msa0230750;Name=Msa0230750 |
| chr2_2 | mRNA | 2943130 | 2943648 | 2943130 | ID=Msa0230750-mRNA-1;Parent=Msa0230750;Name=Msa0230750-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|-1|0|1|-1|1|1|0|172 |
| chr2_2 | exon | 2943130 | 2943648 | 2943130 | ID=Msa0230750-mRNA-1:exon:1051;Parent=Msa0230750-mRNA-1 |
| chr2_2 | CDS | 2943130 | 2943648 | 2943130 | ID=Msa0230750-mRNA-1:cds;Parent=Msa0230750-mRNA-1 |
| Gene Sequence |
| Protein sequence |
