Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0570930 | XP_013446955.1 | 96.454 | 282 | 6 | 1 | 1 | 282 | 1 | 278 | 0.0 | 551 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0570930 | sp|Q9FGQ2|SWT13_ARATH | 47.475 | 297 | 134 | 6 | 1 | 279 | 1 | 293 | 8.56e-85 | 258 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0570930 | I3T0T2 | 96.454 | 282 | 6 | 1 | 1 | 282 | 1 | 278 | 0.0 | 551 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0025690 | Msa0570930 | 0.801585 | 8.400409e-49 | -8.615850e-47 |
| Msa0050320 | Msa0570930 | 0.806808 | 6.871506e-50 | -8.615850e-47 |
| Msa0053360 | Msa0570930 | 0.801831 | 7.480159e-49 | -8.615850e-47 |
| Msa0110350 | Msa0570930 | 0.804288 | 2.321106e-49 | -8.615850e-47 |
| Msa0154720 | Msa0570930 | 0.805925 | 1.054739e-49 | -8.615850e-47 |
| Msa0183960 | Msa0570930 | 0.812322 | 4.493809e-51 | -8.615850e-47 |
| Msa0198140 | Msa0570930 | 0.813806 | 2.124411e-51 | -8.615850e-47 |
| Msa0198220 | Msa0570930 | 0.813621 | 2.333554e-51 | -8.615850e-47 |
| Msa0206600 | Msa0570930 | 0.800906 | 1.156853e-48 | -8.615850e-47 |
| Msa0218430 | Msa0570930 | 0.802102 | 6.580024e-49 | -8.615850e-47 |
| Msa0232660 | Msa0570930 | 0.806708 | 7.213488e-50 | -8.615850e-47 |
| Msa0244370 | Msa0570930 | 0.824697 | 7.015020e-54 | -8.615850e-47 |
| Msa0278340 | Msa0570930 | 0.813133 | 2.985676e-51 | -8.615850e-47 |
| Msa0282880 | Msa0570930 | 0.831351 | 1.753401e-55 | -8.615850e-47 |
| Msa0315390 | Msa0570930 | 0.803908 | 2.784237e-49 | -8.615850e-47 |
| Msa0321180 | Msa0570930 | 0.836526 | 8.889369e-57 | -8.615850e-47 |
| Msa0321200 | Msa0570930 | 0.835390 | 1.725622e-56 | -8.615850e-47 |
| Msa0358720 | Msa0570930 | 0.807356 | 5.261465e-50 | -8.615850e-47 |
| Msa0483160 | Msa0570930 | 0.800876 | 1.173155e-48 | -8.615850e-47 |
| Msa0523140 | Msa0570930 | 0.812195 | 4.789200e-51 | -8.615850e-47 |
| Msa0523150 | Msa0570930 | 0.805751 | 1.147372e-49 | -8.615850e-47 |
| Msa0545750 | Msa0570930 | 0.836319 | 1.003146e-56 | -8.615850e-47 |
| Msa0549550 | Msa0570930 | 0.814106 | 1.824069e-51 | -8.615850e-47 |
| Msa0553570 | Msa0570930 | 0.804214 | 2.405397e-49 | -8.615850e-47 |
| Msa0554410 | Msa0570930 | 0.836177 | 1.090482e-56 | -8.615850e-47 |
| Msa0568050 | Msa0570930 | 0.832132 | 1.125684e-55 | -8.615850e-47 |
| Msa0568120 | Msa0570930 | 0.822529 | 2.255686e-53 | -8.615850e-47 |
| Msa0568260 | Msa0570930 | 0.834673 | 2.615883e-56 | -8.615850e-47 |
| Msa0570250 | Msa0570930 | 0.865225 | 6.889963e-65 | -8.615850e-47 |
| Msa0570310 | Msa0570930 | 0.807904 | 4.024448e-50 | -8.615850e-47 |
| Msa0570930 | Msa0587070 | 0.816614 | 5.049895e-52 | -8.615850e-47 |
| Msa0570930 | Msa0601470 | 0.818928 | 1.517088e-52 | -8.615850e-47 |
| Msa0570930 | Msa0604890 | 0.839817 | 1.262717e-57 | -8.615850e-47 |
| Msa0570930 | Msa0635070 | 0.830595 | 2.687995e-55 | -8.615850e-47 |
| Msa0570930 | Msa0646180 | 0.837910 | 3.932895e-57 | -8.615850e-47 |
| Msa0570930 | Msa0648160 | 0.839427 | 1.594794e-57 | -8.615850e-47 |
| Msa0570930 | Msa0648260 | 0.839630 | 1.412714e-57 | -8.615850e-47 |
| Msa0570930 | Msa0654400 | 0.804600 | 1.998566e-49 | -8.615850e-47 |
| Msa0570930 | Msa0691180 | 0.802836 | 4.643026e-49 | -8.615850e-47 |
| Msa0570930 | Msa0722660 | 0.802279 | 6.048689e-49 | -8.615850e-47 |
| Msa0570930 | Msa0734190 | 0.804077 | 2.567928e-49 | -8.615850e-47 |
| Msa0570930 | Msa0807130 | 0.805684 | 1.185084e-49 | -8.615850e-47 |
| Msa0570930 | Msa0829770 | 0.809668 | 1.688798e-50 | -8.615850e-47 |
| Msa0570930 | Msa0906100 | 0.808853 | 2.524953e-50 | -8.615850e-47 |
| Msa0570930 | Msa0924690 | 0.811478 | 6.861743e-51 | -8.615850e-47 |
| Msa0570930 | Msa0963800 | 0.815423 | 9.314915e-52 | -8.615850e-47 |
| Msa0570930 | Msa0978290 | 0.811542 | 6.647281e-51 | -8.615850e-47 |
| Msa0570930 | Msa0984820 | 0.804088 | 2.555152e-49 | -8.615850e-47 |
| Msa0570930 | Msa1029570 | 0.814883 | 1.227822e-51 | -8.615850e-47 |
| Msa0570930 | Msa1055710 | 0.825914 | 3.614013e-54 | -8.615850e-47 |
| Msa0570930 | Msa1089130 | 0.811903 | 5.545649e-51 | -8.615850e-47 |
| Msa0570930 | Msa1090020 | 0.828389 | 9.237653e-55 | -8.615850e-47 |
| Msa0570930 | Msa1134430 | 0.803332 | 3.666191e-49 | -8.615850e-47 |
| Msa0570930 | Msa1181370 | 0.811269 | 7.619609e-51 | -8.615850e-47 |
| Msa0570930 | Msa1207710 | 0.817816 | 2.710307e-52 | -8.615850e-47 |
| Msa0570930 | Msa1207760 | 0.806461 | 8.132722e-50 | -8.615850e-47 |
| Msa0570930 | Msa1213470 | 0.800548 | 1.368653e-48 | -8.615850e-47 |
| Msa0570930 | Msa1257930 | 0.805120 | 1.555928e-49 | -8.615850e-47 |
| Msa0570930 | Msa1257960 | 0.808746 | 2.662149e-50 | -8.615850e-47 |
| Msa0570930 | Msa1262740 | 0.811763 | 5.949267e-51 | -8.615850e-47 |
| Msa0570930 | Msa1279310 | 0.807255 | 5.526693e-50 | -8.615850e-47 |
| Msa0570930 | Msa1298980 | 0.814041 | 1.884870e-51 | -8.615850e-47 |
| Msa0570930 | Msa1300370 | 0.801413 | 9.109634e-49 | -8.615850e-47 |
| Msa0570930 | Msa1305970 | 0.813271 | 2.784933e-51 | -8.615850e-47 |
| Msa0570930 | Msa1329660 | 0.809053 | 2.288271e-50 | -8.615850e-47 |
| Msa0570930 | Msa1359880 | 0.839039 | 2.011198e-57 | -8.615850e-47 |
| Msa0570930 | Msa1371260 | 0.806850 | 6.732153e-50 | -8.615850e-47 |
| Msa0570930 | Msa1395360 | 0.802526 | 5.380068e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0570930 | MtrunA17_Chr8g0386001 | 96.454 | 282 | 6 | 1 | 1 | 282 | 1 | 278 | 0.0 | 551 |
| Msa0570930 | MtrunA17_Chr3g0131951 | 57.343 | 286 | 102 | 2 | 10 | 275 | 72 | 357 | 3.90e-118 | 343 |
| Msa0570930 | MtrunA17_Chr7g0214191 | 47.122 | 278 | 131 | 3 | 1 | 273 | 1 | 267 | 6.92e-78 | 237 |
| Msa0570930 | MtrunA17_Chr5g0428281 | 45.421 | 273 | 132 | 4 | 3 | 267 | 4 | 267 | 2.95e-74 | 228 |
| Msa0570930 | MtrunA17_Chr7g0214181 | 49.576 | 236 | 118 | 1 | 1 | 236 | 1 | 235 | 8.19e-74 | 227 |
| Msa0570930 | MtrunA17_Chr8g0386011 | 49.550 | 222 | 109 | 3 | 4 | 224 | 5 | 224 | 1.82e-69 | 215 |
| Msa0570930 | MtrunA17_Chr3g0131961 | 46.637 | 223 | 116 | 3 | 10 | 231 | 11 | 231 | 5.05e-67 | 209 |
| Msa0570930 | MtrunA17_Chr7g0215601 | 47.934 | 242 | 121 | 4 | 4 | 241 | 5 | 245 | 8.95e-67 | 208 |
| Msa0570930 | MtrunA17_Chr5g0444031 | 40.891 | 247 | 140 | 3 | 4 | 246 | 25 | 269 | 9.91e-61 | 193 |
| Msa0570930 | MtrunA17_Chr2g0278491 | 42.920 | 226 | 121 | 6 | 56 | 277 | 1 | 222 | 1.70e-50 | 166 |
| Msa0570930 | MtrunA17_Chr2g0295681 | 38.462 | 208 | 127 | 1 | 10 | 217 | 6 | 212 | 1.08e-46 | 156 |
| Msa0570930 | MtrunA17_Chr3g0125861 | 34.498 | 229 | 144 | 3 | 6 | 231 | 2 | 227 | 4.45e-42 | 145 |
| Msa0570930 | MtrunA17_Chr6g0450941 | 37.156 | 218 | 131 | 4 | 11 | 223 | 83 | 299 | 8.81e-42 | 145 |
| Msa0570930 | MtrunA17_Chr8g0388621 | 33.043 | 230 | 151 | 2 | 11 | 238 | 12 | 240 | 1.48e-41 | 143 |
| Msa0570930 | MtrunA17_Chr1g0159431 | 35.931 | 231 | 140 | 4 | 6 | 233 | 2 | 227 | 3.56e-41 | 142 |
| Msa0570930 | MtrunA17_Chr3g0126421 | 32.946 | 258 | 159 | 4 | 5 | 258 | 2 | 249 | 4.74e-41 | 142 |
| Msa0570930 | MtrunA17_Chr3g0119951 | 31.939 | 263 | 164 | 6 | 12 | 269 | 11 | 263 | 5.99e-41 | 142 |
| Msa0570930 | MtrunA17_Chr3g0119991 | 31.474 | 251 | 157 | 6 | 24 | 269 | 2 | 242 | 7.09e-38 | 134 |
| Msa0570930 | MtrunA17_Chr3g0126411 | 32.946 | 258 | 159 | 4 | 5 | 258 | 2 | 249 | 8.26e-38 | 134 |
| Msa0570930 | MtrunA17_Chr1g0158931 | 34.978 | 223 | 136 | 3 | 12 | 226 | 9 | 230 | 1.78e-37 | 133 |
| Msa0570930 | MtrunA17_Chr4g0059191 | 34.123 | 211 | 132 | 3 | 25 | 231 | 1 | 208 | 5.27e-35 | 125 |
| Msa0570930 | MtrunA17_Chr8g0359261 | 33.180 | 217 | 136 | 4 | 13 | 225 | 18 | 229 | 2.63e-32 | 119 |
| Msa0570930 | MtrunA17_Chr6g0450931 | 33.831 | 201 | 121 | 5 | 31 | 223 | 7 | 203 | 3.80e-32 | 118 |
| Msa0570930 | MtrunA17_Chr2g0314571 | 31.963 | 219 | 137 | 5 | 13 | 225 | 18 | 230 | 2.51e-29 | 111 |
| Msa0570930 | MtrunA17_Chr6g0450951 | 34.896 | 192 | 117 | 5 | 31 | 217 | 7 | 195 | 4.21e-29 | 110 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0570930 | AT5G50800.1 | 47.475 | 297 | 134 | 6 | 1 | 279 | 1 | 293 | 8.70e-86 | 258 |
| Msa0570930 | AT5G23660.1 | 53.138 | 239 | 103 | 2 | 1 | 231 | 3 | 240 | 2.74e-85 | 257 |
| Msa0570930 | AT3G48740.1 | 50.628 | 239 | 109 | 3 | 1 | 231 | 3 | 240 | 9.62e-80 | 243 |
| Msa0570930 | AT4G25010.1 | 47.464 | 276 | 138 | 4 | 1 | 272 | 1 | 273 | 1.14e-79 | 243 |
| Msa0570930 | AT5G13170.1 | 44.280 | 271 | 141 | 5 | 10 | 278 | 12 | 274 | 7.98e-78 | 238 |
| Msa0570930 | AT5G50790.1 | 41.026 | 273 | 145 | 6 | 11 | 269 | 11 | 281 | 3.09e-69 | 216 |
| Msa0570930 | AT2G39060.1 | 44.980 | 249 | 130 | 3 | 10 | 254 | 10 | 255 | 2.36e-65 | 205 |
| Msa0570930 | AT4G15920.1 | 35.021 | 237 | 152 | 2 | 8 | 243 | 4 | 239 | 1.32e-51 | 169 |
| Msa0570930 | AT3G16690.1 | 32.710 | 214 | 144 | 0 | 10 | 223 | 6 | 219 | 2.56e-44 | 150 |
| Msa0570930 | AT3G16690.3 | 32.710 | 214 | 144 | 0 | 10 | 223 | 41 | 254 | 3.89e-44 | 151 |
| Msa0570930 | AT1G21460.1 | 36.752 | 234 | 136 | 4 | 6 | 230 | 2 | 232 | 2.00e-42 | 146 |
| Msa0570930 | AT5G62850.1 | 33.778 | 225 | 146 | 2 | 6 | 228 | 5 | 228 | 2.75e-39 | 137 |
| Msa0570930 | AT5G53190.1 | 30.620 | 258 | 171 | 3 | 12 | 265 | 9 | 262 | 1.49e-38 | 136 |
| Msa0570930 | AT4G10850.1 | 33.654 | 208 | 136 | 1 | 11 | 216 | 12 | 219 | 2.01e-38 | 136 |
| Msa0570930 | AT5G40260.1 | 34.579 | 214 | 134 | 4 | 10 | 218 | 9 | 221 | 2.65e-35 | 127 |
| Msa0570930 | AT3G28007.1 | 36.321 | 212 | 128 | 4 | 11 | 218 | 10 | 218 | 3.89e-35 | 127 |
| Msa0570930 | AT1G66770.1 | 31.963 | 219 | 146 | 2 | 1 | 216 | 1 | 219 | 5.70e-35 | 127 |
| Msa0570930 | AT3G14770.1 | 32.569 | 218 | 141 | 3 | 11 | 225 | 16 | 230 | 1.25e-31 | 117 |
| Msa0570930 | AT5G62850.2 | 33.684 | 190 | 123 | 2 | 5 | 192 | 4 | 192 | 3.01e-31 | 116 |
| Msa0570930 | AT5G40260.2 | 35.519 | 183 | 113 | 3 | 10 | 188 | 9 | 190 | 4.53e-30 | 112 |
| Msa0570930 | AT1G21460.2 | 35.417 | 192 | 118 | 3 | 6 | 194 | 2 | 190 | 1.45e-29 | 111 |
| Msa0570930 | AT3G16690.2 | 29.167 | 144 | 102 | 0 | 10 | 153 | 6 | 149 | 6.57e-21 | 88.2 |
Find 59 sgRNAs with CRISPR-Local
Find 132 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTGATTGGGTTGTTTAATT+TGG | 0.148249 | 4_1:+73365425 | Msa0570930:CDS |
| ATTACATACCCTTGCTTTCT+TGG | 0.188827 | 4_1:-73365302 | None:intergenic |
| ATACTCACCATAACGCTTAA+AGG | 0.233412 | 4_1:-73365561 | None:intergenic |
| TGTATGAAATTTCATCATTT+AGG | 0.248527 | 4_1:+73366999 | Msa0570930:three_prime_UTR |
| CTGATTGGGTTGTTTAATTT+GGG | 0.255905 | 4_1:+73365426 | Msa0570930:CDS |
| TTGATATCATCTTTGTGTTC+TGG | 0.282295 | 4_1:-73366510 | None:intergenic |
| TGCAGCACCTTTAAGCGTTA+TGG | 0.323289 | 4_1:+73365554 | Msa0570930:CDS |
| AACTTTGTGGGTATCACATT+TGG | 0.323979 | 4_1:+73366423 | Msa0570930:CDS |
| TCACAACAAGTGCAATTATA+TGG | 0.334912 | 4_1:+73365743 | Msa0570930:CDS |
| AGGCATAAACTCTACACTTT+TGG | 0.335245 | 4_1:-73365701 | None:intergenic |
| TCCCTTTGTATGTTGAATAT+AGG | 0.343813 | 4_1:-73364202 | None:intergenic |
| ACTAACTATGCTATAATATA+CGG | 0.347508 | 4_1:-73366831 | None:intergenic |
| TTCATACATGCATTTATGTA+AGG | 0.347623 | 4_1:-73366984 | None:intergenic |
| ACAGAACGTATCGAACTTCT+TGG | 0.348472 | 4_1:+73365498 | Msa0570930:CDS |
| CACACAACCAAATGCATTGA+TGG | 0.355614 | 4_1:-73365239 | None:intergenic |
| ATCTTCACATTGAAGCTGAT+TGG | 0.360466 | 4_1:+73365411 | Msa0570930:CDS |
| ATCATCACCATCAATGCATT+TGG | 0.361524 | 4_1:+73365232 | Msa0570930:CDS |
| GAAGCAAGAAGAGAAGAAAC+AGG | 0.365263 | 4_1:+73366611 | Msa0570930:CDS |
| CACTCTTCAGTGCAATGCTT+TGG | 0.375576 | 4_1:+73365173 | Msa0570930:CDS |
| TCTTCACATTGAAGCTGATT+GGG | 0.409416 | 4_1:+73365412 | Msa0570930:CDS |
| CCTCAAAGATATATTTGTTA+CGG | 0.420795 | 4_1:+73365783 | Msa0570930:CDS |
| GGGACAGCTTCCGAACTTTG+TGG | 0.421425 | 4_1:+73366410 | Msa0570930:intron |
| CTGAAGAGTGCAGCCACATA+TGG | 0.434896 | 4_1:-73365160 | None:intergenic |
| TGAGAAGTTTGATAAATTGA+AGG | 0.436768 | 4_1:+73366684 | Msa0570930:exon |
| GGACAGCTTCCGAACTTTGT+GGG | 0.454384 | 4_1:+73366411 | Msa0570930:intron |
| CCGTAACAAATATATCTTTG+AGG | 0.464322 | 4_1:-73365783 | None:intergenic |
| AGGAGGAGTGAAAGAGTGAA+AGG | 0.471347 | 4_1:-73365721 | None:intergenic |
| AATGTGATACCCACAAAGTT+CGG | 0.481534 | 4_1:-73366420 | None:intergenic |
| AAATCAAGTACAACAACAAA+AGG | 0.486924 | 4_1:+73366656 | Msa0570930:CDS |
| CTCCTATATTCAACATACAA+AGG | 0.495403 | 4_1:+73364200 | Msa0570930:five_prime_UTR |
| GTTTGTAAGAAGAAAACAAC+GGG | 0.501411 | 4_1:+73365121 | Msa0570930:CDS |
| CTTTGTATGTTGAATATAGG+AGG | 0.505249 | 4_1:-73364199 | None:intergenic |
| TCATACATGCATTTATGTAA+GGG | 0.509666 | 4_1:-73366983 | None:intergenic |
| GAGAAGTTTGATAAATTGAA+GGG | 0.516591 | 4_1:+73366685 | Msa0570930:exon |
| TCCTATATTCAACATACAAA+GGG | 0.522424 | 4_1:+73364201 | Msa0570930:five_prime_UTR |
| CAGCCACATATGGAGCAGAT+TGG | 0.522606 | 4_1:-73365150 | None:intergenic |
| GGAGCAGTAAGTGATATAGA+TGG | 0.535555 | 4_1:-73365281 | None:intergenic |
| TCAAACATATTCTGATACCA+TGG | 0.537188 | 4_1:+73364232 | Msa0570930:exon |
| TTTCCAATCTGCTCCATATG+TGG | 0.537778 | 4_1:+73365147 | Msa0570930:CDS |
| ATTGGGTTGTTTAATTTGGG+AGG | 0.542194 | 4_1:+73365429 | Msa0570930:CDS |
| AACGTATCGAACTTCTTGGA+TGG | 0.564898 | 4_1:+73365502 | Msa0570930:CDS |
| CTTACTGCTCCAAGAAAGCA+AGG | 0.566936 | 4_1:+73365293 | Msa0570930:CDS |
| CACATTTGGAACAATTCAGA+TGG | 0.569161 | 4_1:+73366437 | Msa0570930:CDS |
| TAATTTGGGAGGAATTTGCT+TGG | 0.577927 | 4_1:+73365440 | Msa0570930:CDS |
| AATGCACTAAACAACACACA+TGG | 0.585225 | 4_1:-73366758 | None:intergenic |
| TAATTGCACTTGTTGTGAGG+AGG | 0.606445 | 4_1:-73365738 | None:intergenic |
| CAAGTACAACAACAAAAGGA+AGG | 0.611484 | 4_1:+73366660 | Msa0570930:CDS |
| GAAGAAACAAAACTAAGTGA+AGG | 0.618742 | 4_1:+73366633 | Msa0570930:CDS |
| ATATAATTGCACTTGTTGTG+AGG | 0.624327 | 4_1:-73365741 | None:intergenic |
| GCAATTATATGGTTGTGCTA+TGG | 0.625887 | 4_1:+73365754 | Msa0570930:CDS |
| AGTAAGTGATATAGATGGCG+AGG | 0.639838 | 4_1:-73365276 | None:intergenic |
| AGTATTGTCTCTGTGAACCA+TGG | 0.657818 | 4_1:-73364249 | None:intergenic |
| GGCTATAAAGCAAAAGAAGG+AGG | 0.661005 | 4_1:-73364910 | None:intergenic |
| ATGCACTAAACAACACACAT+GGG | 0.662835 | 4_1:-73366757 | None:intergenic |
| TGTAAGAAGAAAACAACGGG+AGG | 0.667753 | 4_1:+73365124 | Msa0570930:CDS |
| GAGGTGAACATTTAAGCTAG+AGG | 0.674188 | 4_1:+73364141 | Msa0570930:five_prime_UTR |
| TGTTGATATTGAGACTACAG+TGG | 0.725687 | 4_1:+73366575 | Msa0570930:CDS |
| TTACTGCTCCAAGAAAGCAA+GGG | 0.726046 | 4_1:+73365294 | Msa0570930:CDS |
| AGTTTGTAAGAAGAAAACAA+CGG | 0.759066 | 4_1:+73365120 | Msa0570930:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTTTAATTTATATATTA+AGG | + | chr4_1:73366107-73366126 | Msa0570930:intron | 0.0% |
| !!! | AAATAATTATTTTAGTAGTA+TGG | - | chr4_1:73365364-73365383 | None:intergenic | 10.0% |
| !!! | AACAATTTTTTTAGTTTATA+TGG | + | chr4_1:73366312-73366331 | Msa0570930:intron | 10.0% |
| !!! | ACATTATTAATGTTTTTTTT+GGG | + | chr4_1:73366390-73366409 | Msa0570930:intron | 10.0% |
| !! | AGATTTAGTGATAAAAAATA+AGG | + | chr4_1:73364404-73364423 | Msa0570930:intron | 15.0% |
| !!! | TCCAATTTTAAAAAATTAGT+TGG | + | chr4_1:73364549-73364568 | Msa0570930:intron | 15.0% |
| !!! | TCCAACTAATTTTTTAAAAT+TGG | - | chr4_1:73364553-73364572 | None:intergenic | 15.0% |
| !!! | TTTTTTTTGTTTAATGTTGT+AGG | + | chr4_1:73364879-73364898 | Msa0570930:intron | 15.0% |
| !!! | TTTTTTTGAAATACTTACAT+GGG | - | chr4_1:73364936-73364955 | None:intergenic | 15.0% |
| !!! | ATTTTTTTGAAATACTTACA+TGG | - | chr4_1:73364937-73364956 | None:intergenic | 15.0% |
| !!! | AATAGAAATTATAGTTGATA+AGG | + | chr4_1:73366020-73366039 | Msa0570930:intron | 15.0% |
| !!! | TTTTAGTTTATATGGTATTT+TGG | + | chr4_1:73366320-73366339 | Msa0570930:intron | 15.0% |
| !!! | CACATTATTAATGTTTTTTT+TGG | + | chr4_1:73366389-73366408 | Msa0570930:intron | 15.0% |
| !!! | TTTTTTGAAATACTTACATG+GGG | - | chr4_1:73364935-73364954 | None:intergenic | 20.0% |
| !!! | ATTTTATGCCTACATAAAAA+CGG | + | chr4_1:73365198-73365217 | Msa0570930:CDS | 20.0% |
| !! | GAATTAATTACTAAGAGAAT+TGG | - | chr4_1:73366292-73366311 | None:intergenic | 20.0% |
| !! | ACTAACTATGCTATAATATA+CGG | - | chr4_1:73366834-73366853 | None:intergenic | 20.0% |
| !!! | AACAGAGATTACATATTTTT+GGG | - | chr4_1:73366889-73366908 | None:intergenic | 20.0% |
| !!! | AAACAGAGATTACATATTTT+TGG | - | chr4_1:73366890-73366909 | None:intergenic | 20.0% |
| !!! | AAAAAAAGGAAGGTTTTGAA+GGG | - | chr4_1:73364178-73364197 | None:intergenic | 25.0% |
| !!! | AAAAAAAAGGAAGGTTTTGA+AGG | - | chr4_1:73364179-73364198 | None:intergenic | 25.0% |
| ! | TGAATATAGGAGGAAAAAAA+AGG | - | chr4_1:73364192-73364211 | None:intergenic | 25.0% |
| ! | TCCTATATTCAACATACAAA+GGG | + | chr4_1:73364201-73364220 | Msa0570930:five_prime_UTR | 25.0% |
| !!! | ATTTTTGTAGTTGGTATTCT+AGG | + | chr4_1:73364275-73364294 | Msa0570930:CDS | 25.0% |
| !!! | TTTATTTTCCTCTCTAAACT+TGG | + | chr4_1:73364581-73364600 | Msa0570930:intron | 25.0% |
| ! | AAGAGTTTACAATCAAGTTT+AGG | - | chr4_1:73364790-73364809 | None:intergenic | 25.0% |
| ! | GTTAATAAATTCTTGTGAGA+AGG | - | chr4_1:73364838-73364857 | None:intergenic | 25.0% |
| !!! | TTTTTGAAATACTTACATGG+GGG | - | chr4_1:73364934-73364953 | None:intergenic | 25.0% |
| ! | AGTTTGTAAGAAGAAAACAA+CGG | + | chr4_1:73365120-73365139 | Msa0570930:CDS | 25.0% |
| !! | TTTTATGCCTACATAAAAAC+GGG | + | chr4_1:73365199-73365218 | Msa0570930:CDS | 25.0% |
| ! | ATTTGAGAATTGAGTGTTAT+TGG | + | chr4_1:73365380-73365399 | Msa0570930:intron | 25.0% |
| !! | CCTCAAAGATATATTTGTTA+CGG | + | chr4_1:73365783-73365802 | Msa0570930:CDS | 25.0% |
| ! | TTATAGTAGCATAATTATCC+CGG | - | chr4_1:73365836-73365855 | None:intergenic | 25.0% |
| ! | TAGCTAATAAAATTAGCATG+CGG | + | chr4_1:73366043-73366062 | Msa0570930:intron | 25.0% |
| !! | AATTTGGTCATAAGCTTTAA+TGG | - | chr4_1:73366256-73366275 | None:intergenic | 25.0% |
| !!! | AGTTTATATGGTATTTTGGT+TGG | + | chr4_1:73366324-73366343 | Msa0570930:intron | 25.0% |
| ! | GTTAATAGTATCAATCCATT+AGG | - | chr4_1:73366362-73366381 | None:intergenic | 25.0% |
| !!! | TATCAACAACATTTTGAAGA+GGG | - | chr4_1:73366563-73366582 | None:intergenic | 25.0% |
| !!! | ATATCAACAACATTTTGAAG+AGG | - | chr4_1:73366564-73366583 | None:intergenic | 25.0% |
| ! | AAATCAAGTACAACAACAAA+AGG | + | chr4_1:73366656-73366675 | Msa0570930:CDS | 25.0% |
| ! | TGAGAAGTTTGATAAATTGA+AGG | + | chr4_1:73366684-73366703 | Msa0570930:exon | 25.0% |
| ! | GAGAAGTTTGATAAATTGAA+GGG | + | chr4_1:73366685-73366704 | Msa0570930:exon | 25.0% |
| ! | TCATACATGCATTTATGTAA+GGG | - | chr4_1:73366986-73367005 | None:intergenic | 25.0% |
| ! | TTCATACATGCATTTATGTA+AGG | - | chr4_1:73366987-73367006 | None:intergenic | 25.0% |
| TATAGGAGGAAAAAAAAGGA+AGG | - | chr4_1:73364188-73364207 | None:intergenic | 30.0% | |
| CTTTGTATGTTGAATATAGG+AGG | - | chr4_1:73364202-73364221 | None:intergenic | 30.0% | |
| CTCCTATATTCAACATACAA+AGG | + | chr4_1:73364200-73364219 | Msa0570930:five_prime_UTR | 30.0% | |
| TCCCTTTGTATGTTGAATAT+AGG | - | chr4_1:73364205-73364224 | None:intergenic | 30.0% | |
| TCAAACATATTCTGATACCA+TGG | + | chr4_1:73364232-73364251 | Msa0570930:exon | 30.0% | |
| ! | AATACTGCGATTTTTGTAGT+TGG | + | chr4_1:73364266-73364285 | Msa0570930:CDS | 30.0% |
| TGTGACCAAAGAAAATTTCT+TGG | + | chr4_1:73364741-73364760 | Msa0570930:intron | 30.0% | |
| ! | AAGGACCAAGAAATTTTCTT+TGG | - | chr4_1:73364749-73364768 | None:intergenic | 30.0% |
| CAAGTTTAGGAGAAATTCTT+AGG | - | chr4_1:73364777-73364796 | None:intergenic | 30.0% | |
| GTTTGTAAGAAGAAAACAAC+GGG | + | chr4_1:73365121-73365140 | Msa0570930:CDS | 30.0% | |
| ! | CTGATTGGGTTGTTTAATTT+GGG | + | chr4_1:73365426-73365445 | Msa0570930:CDS | 30.0% |
| ATTATTCATGCATAAGCACA+TGG | - | chr4_1:73365649-73365668 | None:intergenic | 30.0% | |
| !! | ATATAATTGCACTTGTTGTG+AGG | - | chr4_1:73365744-73365763 | None:intergenic | 30.0% |
| TCACAACAAGTGCAATTATA+TGG | + | chr4_1:73365743-73365762 | Msa0570930:CDS | 30.0% | |
| CCGTAACAAATATATCTTTG+AGG | - | chr4_1:73365786-73365805 | None:intergenic | 30.0% | |
| !! | CATCTTTAGAAGTGTTACTA+TGG | + | chr4_1:73365908-73365927 | Msa0570930:intron | 30.0% |
| GACAAGTATTATGAAACAGA+GGG | - | chr4_1:73366234-73366253 | None:intergenic | 30.0% | |
| TGGAGATCGGTAAAATAATT+TGG | - | chr4_1:73366272-73366291 | None:intergenic | 30.0% | |
| TTACTAAGAGAATTGGAGAT+CGG | - | chr4_1:73366285-73366304 | None:intergenic | 30.0% | |
| ! | TGGCTTTTATTATGTCCTAA+TGG | + | chr4_1:73366344-73366363 | Msa0570930:intron | 30.0% |
| TTGATATCATCTTTGTGTTC+TGG | - | chr4_1:73366513-73366532 | None:intergenic | 30.0% | |
| GAAGAAACAAAACTAAGTGA+AGG | + | chr4_1:73366633-73366652 | Msa0570930:CDS | 30.0% | |
| GAAAAAAAGATGTAGAGCTG+AGG | - | chr4_1:73364317-73364336 | None:intergenic | 35.0% | |
| CACTCTTGATCTAACCATTA+TGG | + | chr4_1:73364492-73364511 | Msa0570930:intron | 35.0% | |
| TATGATTCACTCGACCATAA+TGG | - | chr4_1:73364509-73364528 | None:intergenic | 35.0% | |
| TGTGACCAAACCAAATTTCT+TGG | - | chr4_1:73364630-73364649 | None:intergenic | 35.0% | |
| AAGAGAGAAGAGAGAGAAAA+TGG | - | chr4_1:73364701-73364720 | None:intergenic | 35.0% | |
| TCTTCTCTCTCTTCTTTCTT+TGG | + | chr4_1:73364717-73364736 | Msa0570930:intron | 35.0% | |
| GTTTAGGAGAAATTCTTAGG+TGG | - | chr4_1:73364774-73364793 | None:intergenic | 35.0% | |
| !! | CATTTCACCCGTTTTTATGT+AGG | - | chr4_1:73365209-73365228 | None:intergenic | 35.0% |
| ATCATCACCATCAATGCATT+TGG | + | chr4_1:73365232-73365251 | Msa0570930:CDS | 35.0% | |
| ATTACATACCCTTGCTTTCT+TGG | - | chr4_1:73365305-73365324 | None:intergenic | 35.0% | |
| ATCTTCACATTGAAGCTGAT+TGG | + | chr4_1:73365411-73365430 | Msa0570930:CDS | 35.0% | |
| TCTTCACATTGAAGCTGATT+GGG | + | chr4_1:73365412-73365431 | Msa0570930:CDS | 35.0% | |
| ! | GCTGATTGGGTTGTTTAATT+TGG | + | chr4_1:73365425-73365444 | Msa0570930:CDS | 35.0% |
| !! | ATTGGGTTGTTTAATTTGGG+AGG | + | chr4_1:73365429-73365448 | Msa0570930:CDS | 35.0% |
| ! | TAATTTGGGAGGAATTTGCT+TGG | + | chr4_1:73365440-73365459 | Msa0570930:CDS | 35.0% |
| ATACTCACCATAACGCTTAA+AGG | - | chr4_1:73365564-73365583 | None:intergenic | 35.0% | |
| CTTTGTTCAATTTCAGAGAG+TGG | + | chr4_1:73365669-73365688 | Msa0570930:intron | 35.0% | |
| ! | AGGCATAAACTCTACACTTT+TGG | - | chr4_1:73365704-73365723 | None:intergenic | 35.0% |
| GCAATTATATGGTTGTGCTA+TGG | + | chr4_1:73365754-73365773 | Msa0570930:CDS | 35.0% | |
| CCAATTTATCCATGACATTC+CGG | + | chr4_1:73365814-73365833 | Msa0570930:intron | 35.0% | |
| CAATTTATCCATGACATTCC+GGG | + | chr4_1:73365815-73365834 | Msa0570930:intron | 35.0% | |
| ATAATTATCCCGGAATGTCA+TGG | - | chr4_1:73365826-73365845 | None:intergenic | 35.0% | |
| GGACAAGTATTATGAAACAG+AGG | - | chr4_1:73366235-73366254 | None:intergenic | 35.0% | |
| AATGTGATACCCACAAAGTT+CGG | - | chr4_1:73366423-73366442 | None:intergenic | 35.0% | |
| AACTTTGTGGGTATCACATT+TGG | + | chr4_1:73366423-73366442 | Msa0570930:CDS | 35.0% | |
| ! | CACATTTGGAACAATTCAGA+TGG | + | chr4_1:73366437-73366456 | Msa0570930:CDS | 35.0% |
| TGTTGATATTGAGACTACAG+TGG | + | chr4_1:73366575-73366594 | Msa0570930:CDS | 35.0% | |
| CAAGTACAACAACAAAAGGA+AGG | + | chr4_1:73366660-73366679 | Msa0570930:CDS | 35.0% | |
| ATGCACTAAACAACACACAT+GGG | - | chr4_1:73366760-73366779 | None:intergenic | 35.0% | |
| AATGCACTAAACAACACACA+TGG | - | chr4_1:73366761-73366780 | None:intergenic | 35.0% | |
| GAGGTGAACATTTAAGCTAG+AGG | + | chr4_1:73364141-73364160 | Msa0570930:five_prime_UTR | 40.0% | |
| AGTATTGTCTCTGTGAACCA+TGG | - | chr4_1:73364252-73364271 | None:intergenic | 40.0% | |
| CGAGTGAATCATAAGTCGAT+AGG | + | chr4_1:73364516-73364535 | Msa0570930:intron | 40.0% | |
| GTGAATCATAAGTCGATAGG+TGG | + | chr4_1:73364519-73364538 | Msa0570930:intron | 40.0% | |
| GAGTTTCTCCAAGTTTAGAG+AGG | - | chr4_1:73364592-73364611 | None:intergenic | 40.0% | |
| TGAACCAGGACCAAGAAATT+TGG | + | chr4_1:73364617-73364636 | Msa0570930:intron | 40.0% | |
| CAAACCAAATTTCTTGGTCC+TGG | - | chr4_1:73364624-73364643 | None:intergenic | 40.0% | |
| CAGGACCAAGAAATTTGGTT+TGG | + | chr4_1:73364622-73364641 | Msa0570930:intron | 40.0% | |
| GAGAAATTCTTAGGTGGTCA+AGG | - | chr4_1:73364768-73364787 | None:intergenic | 40.0% | |
| GGCTATAAAGCAAAAGAAGG+AGG | - | chr4_1:73364913-73364932 | None:intergenic | 40.0% | |
| TGTAAGAAGAAAACAACGGG+AGG | + | chr4_1:73365124-73365143 | Msa0570930:CDS | 40.0% | |
| TTTCCAATCTGCTCCATATG+TGG | + | chr4_1:73365147-73365166 | Msa0570930:CDS | 40.0% | |
| CACACAACCAAATGCATTGA+TGG | - | chr4_1:73365242-73365261 | None:intergenic | 40.0% | |
| AGTAAGTGATATAGATGGCG+AGG | - | chr4_1:73365279-73365298 | None:intergenic | 40.0% | |
| GGAGCAGTAAGTGATATAGA+TGG | - | chr4_1:73365284-73365303 | None:intergenic | 40.0% | |
| TTACTGCTCCAAGAAAGCAA+GGG | + | chr4_1:73365294-73365313 | Msa0570930:CDS | 40.0% | |
| ACAGAACGTATCGAACTTCT+TGG | + | chr4_1:73365498-73365517 | Msa0570930:CDS | 40.0% | |
| AACGTATCGAACTTCTTGGA+TGG | + | chr4_1:73365502-73365521 | Msa0570930:CDS | 40.0% | |
| CTGCAAAAACACTAGTTGAG+AGG | - | chr4_1:73365539-73365558 | None:intergenic | 40.0% | |
| TGCATAAGCACATGGTGATT+TGG | - | chr4_1:73365641-73365660 | None:intergenic | 40.0% | |
| !! | TAATTGCACTTGTTGTGAGG+AGG | - | chr4_1:73365741-73365760 | None:intergenic | 40.0% |
| CCGGAATGTCATGGATAAAT+TGG | - | chr4_1:73365817-73365836 | None:intergenic | 40.0% | |
| ! | AAGTGTTACTATGGCTCAGA+TGG | + | chr4_1:73365917-73365936 | Msa0570930:intron | 40.0% |
| ! | AGTGTTACTATGGCTCAGAT+GGG | + | chr4_1:73365918-73365937 | Msa0570930:intron | 40.0% |
| ! | GGCAGCTTTTGATCATTTAC+AGG | - | chr4_1:73366492-73366511 | None:intergenic | 40.0% |
| GAAGCAAGAAGAGAAGAAAC+AGG | + | chr4_1:73366611-73366630 | Msa0570930:CDS | 40.0% | |
| GAGAAACTCTTGTGTGAACC+AGG | + | chr4_1:73364603-73364622 | Msa0570930:intron | 45.0% | |
| GGGGGCTATAAAGCAAAAGA+AGG | - | chr4_1:73364916-73364935 | None:intergenic | 45.0% | |
| CACTCTTCAGTGCAATGCTT+TGG | + | chr4_1:73365173-73365192 | Msa0570930:CDS | 45.0% | |
| CTTACTGCTCCAAGAAAGCA+AGG | + | chr4_1:73365293-73365312 | Msa0570930:CDS | 45.0% | |
| TGCAGCACCTTTAAGCGTTA+TGG | + | chr4_1:73365554-73365573 | Msa0570930:CDS | 45.0% | |
| AGGAGGAGTGAAAGAGTGAA+AGG | - | chr4_1:73365724-73365743 | None:intergenic | 45.0% | |
| CAGCCACATATGGAGCAGAT+TGG | - | chr4_1:73365153-73365172 | None:intergenic | 50.0% | |
| ! | CTGAAGAGTGCAGCCACATA+TGG | - | chr4_1:73365163-73365182 | None:intergenic | 50.0% |
| GGACAGCTTCCGAACTTTGT+GGG | + | chr4_1:73366411-73366430 | Msa0570930:intron | 50.0% | |
| GGGACAGCTTCCGAACTTTG+TGG | + | chr4_1:73366410-73366429 | Msa0570930:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_1 | gene | 73364135 | 73367021 | 73364135 | ID=Msa0570930;Name=Msa0570930 |
| chr4_1 | mRNA | 73364135 | 73367021 | 73364135 | ID=Msa0570930-mRNA-1;Parent=Msa0570930;Name=Msa0570930-mRNA-1;_AED=0.07;_eAED=0.07;_QI=116|1|1|1|1|1|6|335|282 |
| chr4_1 | exon | 73364135 | 73364296 | 73364135 | ID=Msa0570930-mRNA-1:exon:25134;Parent=Msa0570930-mRNA-1 |
| chr4_1 | exon | 73364901 | 73364937 | 73364901 | ID=Msa0570930-mRNA-1:exon:25135;Parent=Msa0570930-mRNA-1 |
| chr4_1 | exon | 73365105 | 73365315 | 73365105 | ID=Msa0570930-mRNA-1:exon:25136;Parent=Msa0570930-mRNA-1 |
| chr4_1 | exon | 73365411 | 73365575 | 73365411 | ID=Msa0570930-mRNA-1:exon:25137;Parent=Msa0570930-mRNA-1 |
| chr4_1 | exon | 73365685 | 73365804 | 73365685 | ID=Msa0570930-mRNA-1:exon:25138;Parent=Msa0570930-mRNA-1 |
| chr4_1 | exon | 73366417 | 73367021 | 73366417 | ID=Msa0570930-mRNA-1:exon:25139;Parent=Msa0570930-mRNA-1 |
| chr4_1 | five_prime_UTR | 73364135 | 73364250 | 73364135 | ID=Msa0570930-mRNA-1:five_prime_utr;Parent=Msa0570930-mRNA-1 |
| chr4_1 | CDS | 73364251 | 73364296 | 73364251 | ID=Msa0570930-mRNA-1:cds;Parent=Msa0570930-mRNA-1 |
| chr4_1 | CDS | 73364901 | 73364937 | 73364901 | ID=Msa0570930-mRNA-1:cds;Parent=Msa0570930-mRNA-1 |
| chr4_1 | CDS | 73365105 | 73365315 | 73365105 | ID=Msa0570930-mRNA-1:cds;Parent=Msa0570930-mRNA-1 |
| chr4_1 | CDS | 73365411 | 73365575 | 73365411 | ID=Msa0570930-mRNA-1:cds;Parent=Msa0570930-mRNA-1 |
| chr4_1 | CDS | 73365685 | 73365804 | 73365685 | ID=Msa0570930-mRNA-1:cds;Parent=Msa0570930-mRNA-1 |
| chr4_1 | CDS | 73366417 | 73366686 | 73366417 | ID=Msa0570930-mRNA-1:cds;Parent=Msa0570930-mRNA-1 |
| chr4_1 | three_prime_UTR | 73366687 | 73367021 | 73366687 | ID=Msa0570930-mRNA-1:three_prime_utr;Parent=Msa0570930-mRNA-1 |
| Gene Sequence |
| Protein sequence |
