AlfalfaGEDB | Msa0648260

M. sativa cultivar zhongmu-4 / Msa0648260

KEGG: -
GO: -
PFAM: PF03007.19

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0648260	RHN61772.1	98.558	416	6	0	1	416	34	449	0.0	853

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0648260	sp\|Q9M3B3\|WSD4_ARATH	29.613	439	273	9	9	420	18	447	2.86e-53	188

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000610	Msa0648260	0.857162	1.968774e-62	-8.615850e-47
Msa0001040	Msa0648260	0.805043	1.615190e-49	-8.615850e-47
Msa0001510	Msa0648260	0.805455	1.324236e-49	-8.615850e-47
Msa0002110	Msa0648260	0.813292	2.756032e-51	-8.615850e-47
Msa0002690	Msa0648260	0.846524	2.057877e-59	-8.615850e-47
Msa0002700	Msa0648260	0.843738	1.165190e-58	-8.615850e-47
Msa0003450	Msa0648260	0.810543	1.093818e-50	-8.615850e-47
Msa0004340	Msa0648260	0.821745	3.428358e-53	-8.615850e-47
Msa0005500	Msa0648260	0.840419	8.797390e-58	-8.615850e-47
Msa0007570	Msa0648260	0.848486	5.943169e-60	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0648260	MtrunA17_Chr4g0040261	98.558	416	6	0	1	416	34	449	0.0	853
Msa0648260	MtrunA17_Chr4g0040271	92.584	418	30	1	1	418	1	417	0.0	801
Msa0648260	MtrunA17_Chr4g0040251	85.577	416	60	0	1	416	1	416	0.0	751
Msa0648260	MtrunA17_Chr4g0040221	70.238	420	117	4	1	416	1	416	0.0	570
Msa0648260	MtrunA17_Chr4g0040011	65.176	425	141	4	1	419	9	432	0.0	550
Msa0648260	MtrunA17_Chr4g0040001	65.035	429	140	5	1	420	1	428	0.0	547
Msa0648260	MtrunA17_Chr4g0040231	63.615	426	144	4	4	420	5	428	0.0	536
Msa0648260	MtrunA17_Chr7g0217051	61.151	417	161	1	5	420	7	423	0.0	531
Msa0648260	MtrunA17_Chr7g0248011	52.358	424	198	4	2	423	3	424	3.70e-151	437
Msa0648260	MtrunA17_Chr6g0457271	57.735	362	148	4	66	423	5	365	5.70e-145	417

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0648260	AT3G49190.1	29.613	439	273	9	9	420	18	447	2.91e-54	188
Msa0648260	AT5G53390.1	29.412	425	273	9	9	415	15	430	2.06e-53	185
Msa0648260	AT5G16350.3	29.829	409	258	10	9	404	7	399	4.57e-53	184
Msa0648260	AT5G12420.2	29.717	424	257	10	9	404	7	417	8.19e-53	183
Msa0648260	AT5G12420.1	29.717	424	257	10	9	404	7	417	1.04e-52	183
Msa0648260	AT5G16350.1	28.169	426	263	9	9	404	7	419	2.57e-51	180
Msa0648260	AT3G49210.1	28.668	443	275	10	9	415	26	463	7.31e-48	171
Msa0648260	AT5G22490.1	29.452	438	265	15	10	420	12	432	2.94e-47	169
Msa0648260	AT3G49200.1	28.111	434	280	11	9	415	26	454	4.73e-46	166
Msa0648260	AT2G38995.2	29.837	429	271	12	9	420	21	436	1.81e-45	164

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Find 104 sgRNAs with CRISPR-Local

Find 347 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAATCAAGTCTAAGCT+TGG	0.484750	4_3:+54708850	Msa0648260:CDS
AAAACATATGAACATAGGGG+TGG	0.715907	4_3:-54708054	None:intergenic
AAAAGGAGAATATGGTTGAA+GGG	0.537806	4_3:-54708684	None:intergenic
AAACTAGAGAGTTAAGATGA+TGG	0.578017	4_3:-54710274	None:intergenic
AACATCAAATTCCACAGGAT+TGG	0.500187	4_3:+54709766	Msa0648260:CDS
AAGATGACAAGACACCTATA+TGG	0.407784	4_3:+54708758	Msa0648260:CDS
AAGTGTGATGGTTTGCGTGA+AGG	0.622326	4_3:-54708628	None:intergenic
AATAAATGATGTGATTACTG+GGG	0.718291	4_3:+54709691	Msa0648260:CDS
AATCAAATGGATTTCCCAAA+GGG	0.450613	4_3:-54708470	None:intergenic
AATCAAGTCTAAGCTTGGAG+TGG	0.650359	4_3:+54708855	Msa0648260:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCCGAAAACATATGAACATA+GGG	-	chr4_3:54708061-54708080	None:intergenic	30.0%
ATCCGAAAACATATGAACAT+AGG	-	chr4_3:54708062-54708081	None:intergenic	30.0%
ACAAAGATGGCAAAATGAAA+TGG	+	chr4_3:54708311-54708330	Msa0648260:CDS	30.0%
TGGCAAAATGAAATGGAAAA+AGG	+	chr4_3:54708318-54708337	Msa0648260:CDS	30.0%
AATGAAATGGAAAAAGGTAG+AGG	+	chr4_3:54708324-54708343	Msa0648260:CDS	30.0%
AAAAGGAGAATATGGTTGAA+GGG	-	chr4_3:54708687-54708706	None:intergenic	30.0%
AAAAAGGAGAATATGGTTGA+AGG	-	chr4_3:54708688-54708707	None:intergenic	30.0%
GGAATTGAAAAAGGAGAATA+TGG	-	chr4_3:54708695-54708714	None:intergenic	30.0%
ATCAGAGAAGGAATTGAAAA+AGG	-	chr4_3:54708704-54708723	None:intergenic	30.0%
TTTCATCAAGAGAGAATGAT+AGG	-	chr4_3:54708828-54708847	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_3	gene	54707912	54710293	54707912	ID=Msa0648260;Name=Msa0648260
chr4_3	mRNA	54707912	54710293	54707912	ID=Msa0648260-mRNA-1;Parent=Msa0648260;Name=Msa0648260-mRNA-1;_AED=0.43;_eAED=0.43;_QI=82\|1\|0.75\|1\|1\|1\|4\|0\|423
chr4_3	exon	54707912	54708176	54707912	ID=Msa0648260-mRNA-1:exon:13881;Parent=Msa0648260-mRNA-1
chr4_3	exon	54708304	54708876	54708304	ID=Msa0648260-mRNA-1:exon:13882;Parent=Msa0648260-mRNA-1
chr4_3	exon	54709689	54710042	54709689	ID=Msa0648260-mRNA-1:exon:13883;Parent=Msa0648260-mRNA-1
chr4_3	exon	54710132	54710293	54710132	ID=Msa0648260-mRNA-1:exon:13884;Parent=Msa0648260-mRNA-1
chr4_3	five_prime_UTR	54707912	54707993	54707912	ID=Msa0648260-mRNA-1:five_prime_utr;Parent=Msa0648260-mRNA-1
chr4_3	CDS	54707994	54708176	54707994	ID=Msa0648260-mRNA-1:cds;Parent=Msa0648260-mRNA-1
chr4_3	CDS	54708304	54708876	54708304	ID=Msa0648260-mRNA-1:cds;Parent=Msa0648260-mRNA-1
chr4_3	CDS	54709689	54710042	54709689	ID=Msa0648260-mRNA-1:cds;Parent=Msa0648260-mRNA-1
chr4_3	CDS	54710132	54710293	54710132	ID=Msa0648260-mRNA-1:cds;Parent=Msa0648260-mRNA-1

Gene Sequence

>Msa0648260

ATGGACAACTTTGAATATGTAACAGAGCCGGTTAGTCCAAGTGGACAATACTTCAACACACCACCCCTATGTTCATATGTTTTCGGATTTTTGGAATCACAAATTCCAATTGATGATTCTCAAACTATGTATTTGTTCCAACATGTCTTCTTACCTATCAATCCACGTTTCTCCTCCATAATGGTGAGAGACAAAGATGGCAAAATGAAATGGAAAAAGGTAGAGGTGAAGCCTGAAGATCATATGCACGTTCCAATATTCCCAAAAAGTGAATCAATTGAATTGTATGATCAATATTTTGATGATTATGTGTCAAAGATTATGATGGAAAGAACACCACAAGACAAACCCCTTTGGGAAATCCATTTGATTAAATATCCAACAAGCAATGCTGAAGGAACTTTGATATTCAAACTTCATCATGCACTTGGTGATGGTTACTCTCTCATGGGTGCTCTTCTATCTTGTCTACAAAGAGCTGATGATCCTTCTCTTCCTCTATCTTTTCCTTCACGCAAACCATCACACTTGTTATCTCCAAAGAAGGGCTTTTTCAAGTGGTTCCCTTCAACCATATTCTCCTTTTTCAATTCCTTCTCTGATTTTGGATGGAGCATAGCCAAAAGCTCAATGATTAAAGATGACAAGACACCTATATGGAATGGTGAGGAAGGAGTTGAGTCTCAGCCTTGTGTACTATCAAACCTATCATTCTCTCTTGATGAAATCAAAACAATCAAGTCTAAGCTTGGAGTGACAATAAATGATGTGATTACTGGGGTCATTTTCTATGGAATTAGGCTATACATGCAAGAAATGGACAAAAAGGCAAGAACATCAAATTCCACAGGATTGGTGTTGCTTAGTACTAGGAATATTGGAAGTTACCAATCAATTCAAGAAATGACAAAGGCTGATTCAAAATCTCCTTGGGGGAACCATATTTCTTTCTTACATGTATCAATCCCCAAGTTAAGCAAGGCCTGTCTATCTAACCCCTTAGAGTTTATTTGGAAAGCACAAAAAATAATCAAGAGGAAGAGAAATACTTTCACTGTTTTTCTCATTGAATGGCTCTTAGATATGGAGCTGAAATTAAGAGGACATGAGGCAGTTGCTAAACATATCTATGGAACATTGAGAAATTCAAGTGTTGTCATATCCAATTTGATTGGGCCAGTGGAACCTATGGCTTTGGCAAACCATCCTGTGAAAGGCTTGTACTTTACAATGACAGGTGGACCTGAGGTAACCATCATCTTAACTCTCTAG

Protein sequence

>Msa0648260

MDNFEYVTEPVSPSGQYFNTPPLCSYVFGFLESQIPIDDSQTMYLFQHVFLPINPRFSSIMVRDKDGKMKWKKVEVKPEDHMHVPIFPKSESIELYDQYFDDYVSKIMMERTPQDKPLWEIHLIKYPTSNAEGTLIFKLHHALGDGYSLMGALLSCLQRADDPSLPLSFPSRKPSHLLSPKKGFFKWFPSTIFSFFNSFSDFGWSIAKSSMIKDDKTPIWNGEEGVESQPCVLSNLSFSLDEIKTIKSKLGVTINDVITGVIFYGIRLYMQEMDKKARTSNSTGLVLLSTRNIGSYQSIQEMTKADSKSPWGNHISFLHVSIPKLSKACLSNPLEFIWKAQKIIKRKRNTFTVFLIEWLLDMELKLRGHEAVAKHIYGTLRNSSVVISNLIGPVEPMALANHPVKGLYFTMTGGPEVTIILTL*