AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0464540


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0464540 MtrunA17_Chr3g0115791 95.946 148 6 0 2 149 15 162 5.06e-103 291
Msa0464540 MtrunA17_Chr5g0425991 54.000 150 50 6 2 146 77 212 1.55e-35 122
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0464540 AT1G78170.1 40.171 117 62 2 32 140 96 212 1.83e-19 81.3
Msa0464540 AT1G22250.1 36.567 134 71 5 11 137 62 188 1.28e-16 73.6
Msa0464540 AT4G08910.1 40.000 120 45 3 31 132 86 196 3.19e-16 72.8

Find 34 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAACTTCATCTATTTAAC+AGG 0.250373 3_3:+64603955 None:intergenic
CATCTATTTAACAGGGAATA+AGG 0.258672 3_3:+64603963 None:intergenic
TCTTGGTTAGTTTCAAATCT+TGG 0.327382 3_3:+64603853 None:intergenic
CAGCTTCTTCTGCTCTAGTT+AGG 0.340324 3_3:-64604087 Msa0464540:exon
AGCAAGTTTAAGAAAGATTC+GGG 0.373167 3_3:-64604293 Msa0464540:CDS
AAGCAAGTTTAAGAAAGATT+CGG 0.375998 3_3:-64604294 Msa0464540:CDS
CATCTCTTTGTAATCTCCTT+CGG 0.379098 3_3:+64604235 None:intergenic
ATCATGTTCTGTCAAACTCT+TGG 0.401354 3_3:+64604334 None:intergenic
AAAGATTCGGGTGGTTTAAA+CGG 0.409779 3_3:-64604281 Msa0464540:CDS
GTTTCTATCGTTGCCCGATC+GGG 0.446753 3_3:+64604482 None:intergenic
TTGTCGCAGGCTTCGTTTCG+AGG 0.449883 3_3:+64604460 None:intergenic
TCATGTTCTGTCAAACTCTT+GGG 0.450709 3_3:+64604335 None:intergenic
AGTGATCAGAAGAAGAATTC+TGG 0.461088 3_3:-64604365 Msa0464540:CDS
TTTCAGCTTTATATACATCA+TGG 0.469520 3_3:-64604024 Msa0464540:three_prime_UTR
GTATCTGCATTCTGCACTGC+AGG 0.484235 3_3:+64604062 None:intergenic
GGTTTCTATCGTTGCCCGAT+CGG 0.513121 3_3:+64604481 None:intergenic
GAGTGCATGAATTTGCAGTT+AGG 0.515352 3_3:+64604149 None:intergenic
GTGTAACACAAGACCCGATC+GGG 0.521832 3_3:-64604495 Msa0464540:CDS
GTTTAAACGGTTCTCAGTCG+TGG 0.546535 3_3:-64604268 Msa0464540:CDS
CTAGATAGATAACATAGGAT+TGG 0.557065 3_3:+64603995 None:intergenic
AATTTGCAGTTAGGACAAGC+AGG 0.557243 3_3:+64604158 None:intergenic
CGAAGGAGATTACAAAGAGA+TGG 0.571998 3_3:-64604234 Msa0464540:CDS
GCTTTATATACATCATGGCA+TGG 0.572428 3_3:-64604019 Msa0464540:three_prime_UTR
CACATGTCAAGTTTAGCTCA+AGG 0.577317 3_3:+64604411 None:intergenic
AAGCTCATGTGATCAGAAGA+AGG 0.583375 3_3:+64604437 None:intergenic
AAGAACTAGATAGATAACAT+AGG 0.595365 3_3:+64603990 None:intergenic
GATCAGAAGAAGGTTGTCGC+AGG 0.608082 3_3:+64604447 None:intergenic
TGTGTAACACAAGACCCGAT+CGG 0.608388 3_3:-64604496 Msa0464540:CDS
TCGTGGTTGTCGACAACCGA+AGG 0.619983 3_3:-64604251 Msa0464540:CDS
AAAACTTCATCTATTTAACA+GGG 0.632853 3_3:+64603956 None:intergenic
ACTTGACATGTGAATCACAA+AGG 0.656443 3_3:-64604400 Msa0464540:CDS
TTGCATGCAGTGTCACATGT+TGG 0.659365 3_3:-64604201 Msa0464540:CDS
AATATTCTGCTCTGTCAACG+TGG 0.677868 3_3:+64603887 None:intergenic
AAGTTTAAGAAAGATTCGGG+TGG 0.705138 3_3:-64604290 Msa0464540:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GAGTAACAATTAATATTTTA+AGG + chr3_3:64604613-64604632 None:intergenic 15.0%
!!! GAGTAACAATTAATATTTTA+AGG + chr3_3:64604613-64604632 None:intergenic 15.0%
!! ATCATAAGATATGATATCAT+TGG + chr3_3:64603869-64603888 None:intergenic 20.0%
!! AAAACTTCATCTATTTAACA+GGG + chr3_3:64604474-64604493 None:intergenic 20.0%
!! ATCATAAGATATGATATCAT+TGG + chr3_3:64603869-64603888 None:intergenic 20.0%
!! AAAACTTCATCTATTTAACA+GGG + chr3_3:64604474-64604493 None:intergenic 20.0%
!!! AATTTTTTTTATGTGCAGTC+AGG - chr3_3:64603897-64603916 Msa0464540:three_prime_UTR 25.0%
!!! ATTTTTTTTATGTGCAGTCA+GGG - chr3_3:64603898-64603917 Msa0464540:three_prime_UTR 25.0%
! AAGCAAGTTTAAGAAAGATT+CGG - chr3_3:64604133-64604152 Msa0464540:CDS 25.0%
!! TTTCAGCTTTATATACATCA+TGG - chr3_3:64604403-64604422 Msa0464540:CDS 25.0%
! AAGAACTAGATAGATAACAT+AGG + chr3_3:64604440-64604459 None:intergenic 25.0%
! GAAAACTTCATCTATTTAAC+AGG + chr3_3:64604475-64604494 None:intergenic 25.0%
! AAGGATTAACAAAAATGTCT+TGG + chr3_3:64604594-64604613 None:intergenic 25.0%
!!! AATTTTTTTTATGTGCAGTC+AGG - chr3_3:64603897-64603916 Msa0464540:three_prime_UTR 25.0%
!!! ATTTTTTTTATGTGCAGTCA+GGG - chr3_3:64603898-64603917 Msa0464540:three_prime_UTR 25.0%
! AAGCAAGTTTAAGAAAGATT+CGG - chr3_3:64604133-64604152 Msa0464540:CDS 25.0%
!! TTTCAGCTTTATATACATCA+TGG - chr3_3:64604403-64604422 Msa0464540:CDS 25.0%
! AAGAACTAGATAGATAACAT+AGG + chr3_3:64604440-64604459 None:intergenic 25.0%
! GAAAACTTCATCTATTTAAC+AGG + chr3_3:64604475-64604494 None:intergenic 25.0%
! AAGGATTAACAAAAATGTCT+TGG + chr3_3:64604594-64604613 None:intergenic 25.0%
AGCAAGTTTAAGAAAGATTC+GGG - chr3_3:64604134-64604153 Msa0464540:CDS 30.0%
! TGCTCTTCAAGAATTTTGAA+GGG + chr3_3:64604316-64604335 None:intergenic 30.0%
CTAGATAGATAACATAGGAT+TGG + chr3_3:64604435-64604454 None:intergenic 30.0%
CATCTATTTAACAGGGAATA+AGG + chr3_3:64604467-64604486 None:intergenic 30.0%
!! TCTTGGTTAGTTTCAAATCT+TGG + chr3_3:64604577-64604596 None:intergenic 30.0%
AGCAAGTTTAAGAAAGATTC+GGG - chr3_3:64604134-64604153 Msa0464540:CDS 30.0%
! TGCTCTTCAAGAATTTTGAA+GGG + chr3_3:64604316-64604335 None:intergenic 30.0%
CTAGATAGATAACATAGGAT+TGG + chr3_3:64604435-64604454 None:intergenic 30.0%
CATCTATTTAACAGGGAATA+AGG + chr3_3:64604467-64604486 None:intergenic 30.0%
!! TCTTGGTTAGTTTCAAATCT+TGG + chr3_3:64604577-64604596 None:intergenic 30.0%
ACTTGACATGTGAATCACAA+AGG - chr3_3:64604027-64604046 Msa0464540:three_prime_UTR 35.0%
AGTGATCAGAAGAAGAATTC+TGG - chr3_3:64604062-64604081 Msa0464540:three_prime_UTR 35.0%
TCATGTTCTGTCAAACTCTT+GGG + chr3_3:64604095-64604114 None:intergenic 35.0%
ATCATGTTCTGTCAAACTCT+TGG + chr3_3:64604096-64604115 None:intergenic 35.0%
AAGTTTAAGAAAGATTCGGG+TGG - chr3_3:64604137-64604156 Msa0464540:CDS 35.0%
AAAGATTCGGGTGGTTTAAA+CGG - chr3_3:64604146-64604165 Msa0464540:CDS 35.0%
CATCTCTTTGTAATCTCCTT+CGG + chr3_3:64604195-64604214 None:intergenic 35.0%
!! AATTTTGAAGGGTTCTGATC+TGG + chr3_3:64604305-64604324 None:intergenic 35.0%
! CTGCTCTTCAAGAATTTTGA+AGG + chr3_3:64604317-64604336 None:intergenic 35.0%
GCTTTATATACATCATGGCA+TGG - chr3_3:64604408-64604427 Msa0464540:CDS 35.0%
ACTTGACATGTGAATCACAA+AGG - chr3_3:64604027-64604046 Msa0464540:three_prime_UTR 35.0%
AGTGATCAGAAGAAGAATTC+TGG - chr3_3:64604062-64604081 Msa0464540:three_prime_UTR 35.0%
TCATGTTCTGTCAAACTCTT+GGG + chr3_3:64604095-64604114 None:intergenic 35.0%
ATCATGTTCTGTCAAACTCT+TGG + chr3_3:64604096-64604115 None:intergenic 35.0%
AAGTTTAAGAAAGATTCGGG+TGG - chr3_3:64604137-64604156 Msa0464540:CDS 35.0%
AAAGATTCGGGTGGTTTAAA+CGG - chr3_3:64604146-64604165 Msa0464540:CDS 35.0%
CATCTCTTTGTAATCTCCTT+CGG + chr3_3:64604195-64604214 None:intergenic 35.0%
!! AATTTTGAAGGGTTCTGATC+TGG + chr3_3:64604305-64604324 None:intergenic 35.0%
! CTGCTCTTCAAGAATTTTGA+AGG + chr3_3:64604317-64604336 None:intergenic 35.0%
GCTTTATATACATCATGGCA+TGG - chr3_3:64604408-64604427 Msa0464540:CDS 35.0%
AAGCTCATGTGATCAGAAGA+AGG + chr3_3:64603993-64604012 None:intergenic 40.0%
! CACATGTCAAGTTTAGCTCA+AGG + chr3_3:64604019-64604038 None:intergenic 40.0%
CGAAGGAGATTACAAAGAGA+TGG - chr3_3:64604193-64604212 Msa0464540:CDS 40.0%
AATTTGCAGTTAGGACAAGC+AGG + chr3_3:64604272-64604291 None:intergenic 40.0%
GAGTGCATGAATTTGCAGTT+AGG + chr3_3:64604281-64604300 None:intergenic 40.0%
AATATTCTGCTCTGTCAACG+TGG + chr3_3:64604543-64604562 None:intergenic 40.0%
AAGCTCATGTGATCAGAAGA+AGG + chr3_3:64603993-64604012 None:intergenic 40.0%
! CACATGTCAAGTTTAGCTCA+AGG + chr3_3:64604019-64604038 None:intergenic 40.0%
CGAAGGAGATTACAAAGAGA+TGG - chr3_3:64604193-64604212 Msa0464540:CDS 40.0%
AATTTGCAGTTAGGACAAGC+AGG + chr3_3:64604272-64604291 None:intergenic 40.0%
GAGTGCATGAATTTGCAGTT+AGG + chr3_3:64604281-64604300 None:intergenic 40.0%
AATATTCTGCTCTGTCAACG+TGG + chr3_3:64604543-64604562 None:intergenic 40.0%
TGTGTAACACAAGACCCGAT+CGG - chr3_3:64603931-64603950 Msa0464540:three_prime_UTR 45.0%
GTTTAAACGGTTCTCAGTCG+TGG - chr3_3:64604159-64604178 Msa0464540:CDS 45.0%
TTGCATGCAGTGTCACATGT+TGG - chr3_3:64604226-64604245 Msa0464540:CDS 45.0%
CAGCTTCTTCTGCTCTAGTT+AGG - chr3_3:64604340-64604359 Msa0464540:CDS 45.0%
TGTGTAACACAAGACCCGAT+CGG - chr3_3:64603931-64603950 Msa0464540:three_prime_UTR 45.0%
GTTTAAACGGTTCTCAGTCG+TGG - chr3_3:64604159-64604178 Msa0464540:CDS 45.0%
TTGCATGCAGTGTCACATGT+TGG - chr3_3:64604226-64604245 Msa0464540:CDS 45.0%
CAGCTTCTTCTGCTCTAGTT+AGG - chr3_3:64604340-64604359 Msa0464540:CDS 45.0%
GTGTAACACAAGACCCGATC+GGG - chr3_3:64603932-64603951 Msa0464540:three_prime_UTR 50.0%
GTTTCTATCGTTGCCCGATC+GGG + chr3_3:64603948-64603967 None:intergenic 50.0%
GGTTTCTATCGTTGCCCGAT+CGG + chr3_3:64603949-64603968 None:intergenic 50.0%
GATCAGAAGAAGGTTGTCGC+AGG + chr3_3:64603983-64604002 None:intergenic 50.0%
GTATCTGCATTCTGCACTGC+AGG + chr3_3:64604368-64604387 None:intergenic 50.0%
GTGTAACACAAGACCCGATC+GGG - chr3_3:64603932-64603951 Msa0464540:three_prime_UTR 50.0%
GTTTCTATCGTTGCCCGATC+GGG + chr3_3:64603948-64603967 None:intergenic 50.0%
GGTTTCTATCGTTGCCCGAT+CGG + chr3_3:64603949-64603968 None:intergenic 50.0%
GATCAGAAGAAGGTTGTCGC+AGG + chr3_3:64603983-64604002 None:intergenic 50.0%
GTATCTGCATTCTGCACTGC+AGG + chr3_3:64604368-64604387 None:intergenic 50.0%
TTGTCGCAGGCTTCGTTTCG+AGG + chr3_3:64603970-64603989 None:intergenic 55.0%
TCGTGGTTGTCGACAACCGA+AGG - chr3_3:64604176-64604195 Msa0464540:CDS 55.0%
TTGTCGCAGGCTTCGTTTCG+AGG + chr3_3:64603970-64603989 None:intergenic 55.0%
TCGTGGTTGTCGACAACCGA+AGG - chr3_3:64604176-64604195 Msa0464540:CDS 55.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 64603810 64604639 64603810 ID=Msa0464540;Name=Msa0464540
chr3_3 mRNA 64603810 64604639 64603810 ID=Msa0464540-mRNA-1;Parent=Msa0464540;Name=Msa0464540-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|0|0.5|1|0|0|2|278|149
chr3_3 exon 64603810 64604534 64603810 ID=Msa0464540-mRNA-1:exon:14251;Parent=Msa0464540-mRNA-1
chr3_3 exon 64604637 64604639 64604637 ID=Msa0464540-mRNA-1:exon:14250;Parent=Msa0464540-mRNA-1
chr3_3 CDS 64604637 64604639 64604637 ID=Msa0464540-mRNA-1:cds;Parent=Msa0464540-mRNA-1
chr3_3 CDS 64604088 64604534 64604088 ID=Msa0464540-mRNA-1:cds;Parent=Msa0464540-mRNA-1
chr3_3 three_prime_UTR 64603810 64604087 64603810 ID=Msa0464540-mRNA-1:three_prime_utr;Parent=Msa0464540-mRNA-1
Gene Sequence

>Msa0464540

TTGTCAGGGAAGATACAGTTGTGTAACACAAGACCCGATCGGGCAACGATAGAAACCTCGAAACGAAGCCTGCGACAACCTTCTTCTGATCACATGAGCTTAGACCTTGAGCTAAACTTGACATGTGAATCACAAAGGAAAAACAAAAACAGTGATCAGAAGAAGAATTCTGGTAATTCTCCCAAGAGTTTGACAGAACATGATGATCTTTGTATTGAATCAAGCAAGTTTAAGAAAGATTCGGGTGGTTTAAACGGTTCTCAGTCGTGGTTGTCGACAACCGAAGGAGATTACAAAGAGATGGTTGCAACAGTTTGCATGCAGTGTCACATGTTGGTAATGATATGCAAGTCATCTCCTGCTTGTCCTAACTGCAAATTCATGCACTCACCAGATCAGAACCCTTCAAAATTCTTGAAGAGCAGATGCAGCTTCTTCTGCTCTAGTTAG

Protein sequence

>Msa0464540

LSGKIQLCNTRPDRATIETSKRSLRQPSSDHMSLDLELNLTCESQRKNKNSDQKKNSGNSPKSLTEHDDLCIESSKFKKDSGGLNGSQSWLSTTEGDYKEMVATVCMQCHMLVMICKSSPACPNCKFMHSPDQNPSKFLKSRCSFFCSS*