AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1181660


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1181660 MtrunA17_Chr8g0339681 93.382 136 9 0 1 136 1 136 8.73e-91 259
Msa1181660 MtrunA17_Chr8g0339701 88.608 79 9 0 1 79 1 79 1.77e-48 150
Msa1181660 MtrunA17_Chr8g0339701 63.235 68 25 0 69 136 12 79 3.22e-26 94.0
Msa1181660 MtrunA17_Chr8g0339721 68.354 79 25 0 1 79 1 79 6.85e-28 98.2
Msa1181660 MtrunA17_Chr8g0339721 64.815 54 19 0 83 136 26 79 2.79e-21 81.6
Msa1181660 MtrunA17_Chr8g0339691 82.353 51 9 0 27 77 1 51 1.34e-26 95.5
Msa1181660 MtrunA17_Chr8g0339691 74.510 51 13 0 84 134 1 51 3.28e-23 87.0
Msa1181660 MtrunA17_Chr8g0339741 55.000 80 35 1 1 79 1 80 8.77e-24 87.8
Msa1181660 MtrunA17_Chr8g0339741 52.727 55 25 1 83 136 26 80 6.59e-14 62.8
Msa1181660 MtrunA17_Chr8g0339711 53.750 80 35 2 1 79 1 79 1.96e-20 79.3
Msa1181660 MtrunA17_Chr8g0339711 60.000 55 21 1 83 136 25 79 4.94e-16 68.2
Msa1181660 MtrunA17_Chr3g0084701 51.899 79 36 1 1 79 1 77 2.04e-19 76.6
Msa1181660 MtrunA17_Chr8g0339651 49.367 79 35 3 4 79 13 89 2.42e-18 74.3
Msa1181660 MtrunA17_Chr8g0339751 58.228 79 32 1 1 79 1 78 2.62e-18 73.9
Msa1181660 MtrunA17_Chr8g0339751 59.649 57 22 1 80 136 23 78 1.46e-16 69.3
Msa1181660 MtrunA17_Chr8g0345161 51.948 77 33 2 3 79 4 76 2.98e-17 71.2
Msa1181660 MtrunA17_Chr8g0357271 51.852 81 34 4 1 79 1 78 3.12e-16 68.6
Msa1181660 MtrunA17_Chr7g0260561 43.902 82 40 4 1 79 1 79 4.72e-16 68.2
Msa1181660 MtrunA17_Chr8g0339571 48.101 79 38 2 1 79 9 84 1.26e-13 62.0
Msa1181660 MtrunA17_Chr8g0339481 45.000 80 43 1 1 79 1 80 5.68e-13 60.1
Msa1181660 MtrunA17_Chr8g0339501 33.607 122 61 3 1 121 1 103 5.96e-13 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1181660 AT1G19610.1 50.000 74 34 1 9 79 5 78 1.05e-18 75.1
Msa1181660 AT1G19610.1 54.717 53 24 0 84 136 26 78 3.12e-12 58.5
Msa1181660 AT2G26020.1 41.772 79 41 1 6 79 2 80 5.09e-12 58.2
Msa1181660 AT5G44420.1 39.241 79 43 1 6 79 2 80 7.05e-11 55.1

Find 24 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCGTCTTTCACAAAGTTT+TGG 0.275703 8_1:+16112160 None:intergenic
GCTTGTCATCGTCAAGGCTT+TGG 0.378692 8_1:-16112444 Msa1181660:CDS
TGCTTGTCACCGTCAAGGCT+TGG 0.420072 8_1:-16112059 Msa1181660:CDS
ATGCAATACCCAAGCCTTGA+CGG 0.445877 8_1:+16112050 None:intergenic
GCTTGTCACCGTCAAGGCTT+GGG 0.447874 8_1:-16112058 Msa1181660:CDS
AATGTGGAGCATGCTACTTT+TGG 0.453095 8_1:-16112468 Msa1181660:CDS
AAGCGAAGTAAAACATGGTC+AGG 0.480655 8_1:-16112531 Msa1181660:CDS
AGACGAAGCAAAACATGGTC+AGG 0.500469 8_1:-16112145 Msa1181660:CDS
AATGTGGAGCATGCAACTTC+TGG 0.503666 8_1:-16112082 Msa1181660:CDS
CAGTTTCCTGTGTTAAGACA+AGG 0.529102 8_1:+16112507 None:intergenic
GTTGTAGAATTTACTAGCAG+AGG 0.536081 8_1:+16112730 None:intergenic
TGATTAATTTGTACCACATG+TGG 0.540054 8_1:+16112670 None:intergenic
TTTGGTGCTTGTCATCGTCA+AGG 0.545774 8_1:-16112450 Msa1181660:CDS
ACATGTGGAAATCAAGAGAA+AGG 0.550595 8_1:+16112685 None:intergenic
TCTGGTGCTTGTCACCGTCA+AGG 0.563470 8_1:-16112064 Msa1181660:CDS
TTTCTCTTGATTTCCACATG+TGG 0.580296 8_1:-16112683 Msa1181660:intron
TAAAAGACAATGCATTAATG+TGG 0.585322 8_1:-16112484 Msa1181660:CDS
CAATGTCCTGAGTTAAGACA+AGG 0.599143 8_1:+16112121 None:intergenic
GTGAAAGACGAAGCAAAACA+TGG 0.629517 8_1:-16112150 Msa1181660:CDS
TCAGGACCTTGTCTTAACTC+AGG 0.650744 8_1:-16112127 Msa1181660:CDS
TAAAAGACAGTGCATCAATG+TGG 0.668011 8_1:-16112098 Msa1181660:CDS
GTCAAAAGCGAAGTAAAACA+TGG 0.672829 8_1:-16112536 Msa1181660:CDS
TGGAAATCAAGAGAAAGGCA+AGG 0.676802 8_1:+16112690 None:intergenic
TCAGGACCTTGTCTTAACAC+AGG 0.703356 8_1:-16112513 Msa1181660:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATGAGAAATTATTAATAT+AGG + chr8_1:16112125-16112144 None:intergenic 10.0%
!! AACAATAAAGCAAATTAATT+AGG + chr8_1:16112167-16112186 None:intergenic 15.0%
!!! TAATTGTTATATTATGGTTT+TGG + chr8_1:16112425-16112444 None:intergenic 15.0%
!!! GCATTTTAATTGTTATATTA+TGG + chr8_1:16112431-16112450 None:intergenic 15.0%
!! AAATGAACAATTATTAACAT+AGG + chr8_1:16112519-16112538 None:intergenic 15.0%
!! AAATTAAGAACACAAAAATA+TGG + chr8_1:16112548-16112567 None:intergenic 15.0%
!!! TTTTTTCTTTTTATATGTAG+AGG - chr8_1:16112576-16112595 Msa1181660:intron 15.0%
!! ATTAATTAGGAACATAGATA+AGG + chr8_1:16112154-16112173 None:intergenic 20.0%
! TAAAAGACAATGCATTAATG+TGG - chr8_1:16112280-16112299 Msa1181660:intron 25.0%
! AATGTTAACGATATACTCTT+TGG + chr8_1:16112377-16112396 None:intergenic 25.0%
!! TTTCTTTTTATATGTAGAGG+TGG - chr8_1:16112579-16112598 Msa1181660:intron 25.0%
!! TGATTAATTTGTACCACATG+TGG + chr8_1:16112097-16112116 None:intergenic 30.0%
GTTGTAGAATTTACTAGCAG+AGG + chr8_1:16112037-16112056 None:intergenic 35.0%
ACATGTGGAAATCAAGAGAA+AGG + chr8_1:16112082-16112101 None:intergenic 35.0%
TTTCTCTTGATTTCCACATG+TGG - chr8_1:16112081-16112100 Msa1181660:CDS 35.0%
TTCTTGCTTTCTATGTGTAG+AGG - chr8_1:16112190-16112209 Msa1181660:intron 35.0%
GTCAAAAGCGAAGTAAAACA+TGG - chr8_1:16112228-16112247 Msa1181660:intron 35.0%
TTATTTGCACCACATGTATC+TGG + chr8_1:16112488-16112507 None:intergenic 35.0%
! CTTCGTCTTTCACAAAGTTT+TGG + chr8_1:16112607-16112626 None:intergenic 35.0%
TAAAAGACAGTGCATCAATG+TGG - chr8_1:16112666-16112685 Msa1181660:intron 35.0%
TGGAAATCAAGAGAAAGGCA+AGG + chr8_1:16112077-16112096 None:intergenic 40.0%
TTGCTTTCTATGTGTAGAGG+TGG - chr8_1:16112193-16112212 Msa1181660:intron 40.0%
AAGCGAAGTAAAACATGGTC+AGG - chr8_1:16112233-16112252 Msa1181660:intron 40.0%
CAGTTTCCTGTGTTAAGACA+AGG + chr8_1:16112260-16112279 None:intergenic 40.0%
!!! AATGTGGAGCATGCTACTTT+TGG - chr8_1:16112296-16112315 Msa1181660:intron 40.0%
GTGAAAGACGAAGCAAAACA+TGG - chr8_1:16112614-16112633 Msa1181660:intron 40.0%
CAATGTCCTGAGTTAAGACA+AGG + chr8_1:16112646-16112665 None:intergenic 40.0%
TCAGGACCTTGTCTTAACAC+AGG - chr8_1:16112251-16112270 Msa1181660:intron 45.0%
!! TTTGGTGCTTGTCATCGTCA+AGG - chr8_1:16112314-16112333 Msa1181660:intron 45.0%
ATGTCACCTCCAGATACATG+TGG - chr8_1:16112476-16112495 Msa1181660:CDS 45.0%
TTTGCACCACATGTATCTGG+AGG + chr8_1:16112485-16112504 None:intergenic 45.0%
AGACGAAGCAAAACATGGTC+AGG - chr8_1:16112619-16112638 Msa1181660:intron 45.0%
TCAGGACCTTGTCTTAACTC+AGG - chr8_1:16112637-16112656 Msa1181660:intron 45.0%
AATGTGGAGCATGCAACTTC+TGG - chr8_1:16112682-16112701 Msa1181660:intron 45.0%
ATGCAATACCCAAGCCTTGA+CGG + chr8_1:16112717-16112736 None:intergenic 45.0%
GCTTGTCATCGTCAAGGCTT+TGG - chr8_1:16112320-16112339 Msa1181660:intron 50.0%
TCTGGTGCTTGTCACCGTCA+AGG - chr8_1:16112700-16112719 Msa1181660:CDS 55.0%
TGCTTGTCACCGTCAAGGCT+TGG - chr8_1:16112705-16112724 Msa1181660:CDS 55.0%
GCTTGTCACCGTCAAGGCTT+GGG - chr8_1:16112706-16112725 Msa1181660:CDS 55.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 16112027 16112759 16112027 ID=Msa1181660;Name=Msa1181660
chr8_1 mRNA 16112027 16112759 16112027 ID=Msa1181660-mRNA-1;Parent=Msa1181660;Name=Msa1181660-mRNA-1;_AED=0.43;_eAED=0.43;_QI=0|0|0|1|1|1|3|0|136
chr8_1 exon 16112684 16112759 16112684 ID=Msa1181660-mRNA-1:exon:6065;Parent=Msa1181660-mRNA-1
chr8_1 exon 16112406 16112576 16112406 ID=Msa1181660-mRNA-1:exon:6064;Parent=Msa1181660-mRNA-1
chr8_1 exon 16112027 16112190 16112027 ID=Msa1181660-mRNA-1:exon:6063;Parent=Msa1181660-mRNA-1
chr8_1 CDS 16112684 16112759 16112684 ID=Msa1181660-mRNA-1:cds;Parent=Msa1181660-mRNA-1
chr8_1 CDS 16112406 16112576 16112406 ID=Msa1181660-mRNA-1:cds;Parent=Msa1181660-mRNA-1
chr8_1 CDS 16112027 16112190 16112027 ID=Msa1181660-mRNA-1:cds;Parent=Msa1181660-mRNA-1
Gene Sequence

>Msa1181660

ATGACTTCCTCTGCTAGTAAATTCTACAACATCTTCATTTTTGTCTGCCTTGCCTTTCTCTTGATTTCCACATGTGAGGTGGAAGCAAAACTTTGTCAAAAGCGAAGTAAAACATGGTCAGGACCTTGTCTTAACACAGGAAACTGTAAAAGACAATGCATTAATGTGGAGCATGCTACTTTTGGTGCTTGTCATCGTCAAGGCTTTGGTTTTGCTTGCTTCTGCTACAGAAAATGCGCTCCAAAGAAGGTGGAACCAAAACTTTGTGAAAGACGAAGCAAAACATGGTCAGGACCTTGTCTTAACTCAGGACATTGTAAAAGACAGTGCATCAATGTGGAGCATGCAACTTCTGGTGCTTGTCACCGTCAAGGCTTGGGTATTGCATGCTTCTGCAAGAAAAAGTGTTGA

Protein sequence

>Msa1181660

MTSSASKFYNIFIFVCLAFLLISTCEVEAKLCQKRSKTWSGPCLNTGNCKRQCINVEHATFGACHRQGFGFACFCYRKCAPKKVEPKLCERRSKTWSGPCLNSGHCKRQCINVEHATSGACHRQGLGIACFCKKKC*