Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1389900 | XP_003629616.1 | 95.798 | 119 | 4 | 1 | 1 | 118 | 1 | 119 | 1.12e-76 | 232 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1389900 | sp|O23262|RLF32_ARATH | 46.087 | 115 | 47 | 5 | 10 | 118 | 12 | 117 | 1.04e-24 | 93.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1389900 | B7FMN1 | 95.798 | 119 | 4 | 1 | 1 | 118 | 1 | 119 | 5.35e-77 | 232 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0017930 | Msa1389900 | 0.810583 | 1.072360e-50 | -8.615850e-47 |
| Msa0077340 | Msa1389900 | 0.836447 | 9.310122e-57 | -8.615850e-47 |
| Msa0077560 | Msa1389900 | 0.805250 | 1.461860e-49 | -8.615850e-47 |
| Msa0093850 | Msa1389900 | 0.833672 | 4.662475e-56 | -8.615850e-47 |
| Msa0105650 | Msa1389900 | 0.801642 | 8.175599e-49 | -8.615850e-47 |
| Msa0106620 | Msa1389900 | 0.802894 | 4.516455e-49 | -8.615850e-47 |
| Msa0106710 | Msa1389900 | 0.802771 | 4.790222e-49 | -8.615850e-47 |
| Msa1348090 | Msa1389900 | 0.806757 | 7.043140e-50 | -8.615850e-47 |
| Msa1348170 | Msa1389900 | -0.815417 | 9.346862e-52 | -8.615850e-47 |
| Msa1356600 | Msa1389900 | -0.841592 | 4.327493e-58 | -8.615850e-47 |
| Msa1368390 | Msa1389900 | 0.804594 | 2.004089e-49 | -8.615850e-47 |
| Msa1382300 | Msa1389900 | 0.818923 | 1.520948e-52 | -8.615850e-47 |
| Msa1382710 | Msa1389900 | 0.801678 | 8.038224e-49 | -8.615850e-47 |
| Msa1389900 | Msa1391500 | 0.809336 | 1.989886e-50 | -8.615850e-47 |
| Msa1389900 | Msa1398810 | 0.801079 | 1.066150e-48 | -8.615850e-47 |
| Msa1389900 | Msa1401670 | 0.811621 | 6.389152e-51 | -8.615850e-47 |
| Msa1389900 | Msa1405460 | 0.817930 | 2.554251e-52 | -8.615850e-47 |
| Msa1389900 | Msa1407750 | 0.831194 | 1.917021e-55 | -8.615850e-47 |
| Msa1389900 | Msa1412000 | 0.817522 | 3.157600e-52 | -8.615850e-47 |
| Msa1389900 | Msa1427110 | 0.812249 | 4.661293e-51 | -8.615850e-47 |
| Msa1389900 | Msa1439250 | 0.802991 | 4.314434e-49 | -8.615850e-47 |
| Msa1389900 | Msa1459360 | 0.807000 | 6.258446e-50 | -8.615850e-47 |
| Msa1389900 | Msa1461370 | 0.828466 | 8.849010e-55 | -8.615850e-47 |
| Msa1389900 | Msa1464090 | 0.811276 | 7.591428e-51 | -8.615850e-47 |
| Msa0610690 | Msa1389900 | 0.819033 | 1.436016e-52 | -8.615850e-47 |
| Msa0611210 | Msa1389900 | 0.809848 | 1.545111e-50 | -8.615850e-47 |
| Msa0619050 | Msa1389900 | 0.802609 | 5.172323e-49 | -8.615850e-47 |
| Msa0640110 | Msa1389900 | 0.809122 | 2.211491e-50 | -8.615850e-47 |
| Msa0651550 | Msa1389900 | 0.803498 | 3.387452e-49 | -8.615850e-47 |
| Msa0674480 | Msa1389900 | 0.864170 | 1.474137e-64 | -8.615850e-47 |
| Msa0693330 | Msa1389900 | 0.840479 | 8.484077e-58 | -8.615850e-47 |
| Msa0693340 | Msa1389900 | -0.837753 | 4.315006e-57 | -8.615850e-47 |
| Msa0702720 | Msa1389900 | 0.844589 | 6.885590e-59 | -8.615850e-47 |
| Msa0703800 | Msa1389900 | 0.814416 | 1.557679e-51 | -8.615850e-47 |
| Msa0703950 | Msa1389900 | 0.802073 | 6.668503e-49 | -8.615850e-47 |
| Msa0723090 | Msa1389900 | 0.803343 | 3.646982e-49 | -8.615850e-47 |
| Msa0734250 | Msa1389900 | 0.805212 | 1.488565e-49 | -8.615850e-47 |
| Msa0795750 | Msa1389900 | 0.837073 | 6.446766e-57 | -8.615850e-47 |
| Msa0833530 | Msa1389900 | 0.834256 | 3.328691e-56 | -8.615850e-47 |
| Msa0125360 | Msa1389900 | 0.814005 | 1.919754e-51 | -8.615850e-47 |
| Msa0140770 | Msa1389900 | -0.804373 | 2.228398e-49 | -8.615850e-47 |
| Msa0142430 | Msa1389900 | 0.805313 | 1.418128e-49 | -8.615850e-47 |
| Msa0202490 | Msa1389900 | 0.816141 | 6.442621e-52 | -8.615850e-47 |
| Msa0204950 | Msa1389900 | 0.802108 | 6.560921e-49 | -8.615850e-47 |
| Msa0209140 | Msa1389900 | 0.837909 | 3.934602e-57 | -8.615850e-47 |
| Msa1225860 | Msa1389900 | -0.816682 | 4.876391e-52 | -8.615850e-47 |
| Msa1258000 | Msa1389900 | 0.803425 | 3.507981e-49 | -8.615850e-47 |
| Msa1327870 | Msa1389900 | 0.846132 | 2.631680e-59 | -8.615850e-47 |
| Msa1340200 | Msa1389900 | 0.809485 | 1.848422e-50 | -8.615850e-47 |
| Msa1341840 | Msa1389900 | 0.832774 | 7.802647e-56 | -8.615850e-47 |
| Msa0365210 | Msa1389900 | 0.808253 | 3.391145e-50 | -8.615850e-47 |
| Msa0370490 | Msa1389900 | 0.800018 | 1.754835e-48 | -8.615850e-47 |
| Msa0375330 | Msa1389900 | -0.819673 | 1.026250e-52 | -8.615850e-47 |
| Msa0375640 | Msa1389900 | 0.833723 | 4.527178e-56 | -8.615850e-47 |
| Msa0412790 | Msa1389900 | 0.808125 | 3.611445e-50 | -8.615850e-47 |
| Msa0417970 | Msa1389900 | 0.839101 | 1.938330e-57 | -8.615850e-47 |
| Msa0424160 | Msa1389900 | 0.842420 | 2.614984e-58 | -8.615850e-47 |
| Msa0427700 | Msa1389900 | 0.805756 | 1.144754e-49 | -8.615850e-47 |
| Msa0479760 | Msa1389900 | -0.803242 | 3.828086e-49 | -8.615850e-47 |
| Msa1034860 | Msa1389900 | 0.807427 | 5.081063e-50 | -8.615850e-47 |
| Msa1044250 | Msa1389900 | 0.802402 | 5.707264e-49 | -8.615850e-47 |
| Msa1072940 | Msa1389900 | 0.830452 | 2.914173e-55 | -8.615850e-47 |
| Msa1074900 | Msa1389900 | 0.824841 | 6.486577e-54 | -8.615850e-47 |
| Msa1089450 | Msa1389900 | 0.824347 | 8.478621e-54 | -8.615850e-47 |
| Msa1106590 | Msa1389900 | 0.811036 | 8.557192e-51 | -8.615850e-47 |
| Msa0240900 | Msa1389900 | 0.830733 | 2.486589e-55 | -8.615850e-47 |
| Msa0241610 | Msa1389900 | 0.808206 | 3.471482e-50 | -8.615850e-47 |
| Msa0261170 | Msa1389900 | 0.818173 | 2.250265e-52 | -8.615850e-47 |
| Msa0263230 | Msa1389900 | 0.820042 | 8.450810e-53 | -8.615850e-47 |
| Msa0281470 | Msa1389900 | 0.846427 | 2.186364e-59 | -8.615850e-47 |
| Msa0284180 | Msa1389900 | 0.812728 | 3.663557e-51 | -8.615850e-47 |
| Msa0288620 | Msa1389900 | 0.837669 | 4.535222e-57 | -8.615850e-47 |
| Msa0304570 | Msa1389900 | 0.823903 | 1.078194e-53 | -8.615850e-47 |
| Msa0304680 | Msa1389900 | 0.806583 | 7.665935e-50 | -8.615850e-47 |
| Msa0305780 | Msa1389900 | 0.817869 | 2.636463e-52 | -8.615850e-47 |
| Msa0307270 | Msa1389900 | 0.844267 | 8.404206e-59 | -8.615850e-47 |
| Msa0313900 | Msa1389900 | 0.804568 | 2.029286e-49 | -8.615850e-47 |
| Msa0317970 | Msa1389900 | 0.812901 | 3.357509e-51 | -8.615850e-47 |
| Msa0320500 | Msa1389900 | 0.800345 | 1.505519e-48 | -8.615850e-47 |
| Msa0322900 | Msa1389900 | 0.812757 | 3.610317e-51 | -8.615850e-47 |
| Msa0326710 | Msa1389900 | 0.833265 | 5.888061e-56 | -8.615850e-47 |
| Msa0328730 | Msa1389900 | 0.800471 | 1.419250e-48 | -8.615850e-47 |
| Msa0336710 | Msa1389900 | 0.820465 | 6.760987e-53 | -8.615850e-47 |
| Msa0338340 | Msa1389900 | 0.801877 | 7.319156e-49 | -8.615850e-47 |
| Msa0339020 | Msa1389900 | 0.806106 | 9.664301e-50 | -8.615850e-47 |
| Msa0341690 | Msa1389900 | 0.844081 | 9.426713e-59 | -8.615850e-47 |
| Msa1160920 | Msa1389900 | 0.819881 | 9.198960e-53 | -8.615850e-47 |
| Msa1165090 | Msa1389900 | 0.811401 | 7.132509e-51 | -8.615850e-47 |
| Msa1190180 | Msa1389900 | 0.830539 | 2.774326e-55 | -8.615850e-47 |
| Msa0515800 | Msa1389900 | 0.833533 | 5.049499e-56 | -8.615850e-47 |
| Msa0519760 | Msa1389900 | 0.819692 | 1.016079e-52 | -8.615850e-47 |
| Msa0519810 | Msa1389900 | 0.819226 | 1.297745e-52 | -8.615850e-47 |
| Msa0519840 | Msa1389900 | 0.822553 | 2.227799e-53 | -8.615850e-47 |
| Msa0529030 | Msa1389900 | 0.813339 | 2.691185e-51 | -8.615850e-47 |
| Msa0543560 | Msa1389900 | 0.830156 | 3.442325e-55 | -8.615850e-47 |
| Msa0567380 | Msa1389900 | 0.814522 | 1.476185e-51 | -8.615850e-47 |
| Msa0579160 | Msa1389900 | 0.815217 | 1.035200e-51 | -8.615850e-47 |
| Msa0579220 | Msa1389900 | 0.813880 | 2.046238e-51 | -8.615850e-47 |
| Msa0583360 | Msa1389900 | -0.822058 | 2.902565e-53 | -8.615850e-47 |
| Msa0599420 | Msa1389900 | 0.821379 | 4.165974e-53 | -8.615850e-47 |
| Msa0851850 | Msa1389900 | 0.833445 | 5.311527e-56 | -8.615850e-47 |
| Msa0870460 | Msa1389900 | -0.818550 | 1.849260e-52 | -8.615850e-47 |
| Msa0945190 | Msa1389900 | 0.801332 | 9.464025e-49 | -8.615850e-47 |
| Msa0979180 | Msa1389900 | 0.808700 | 2.722382e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1389900 | MtrunA17_Chr8g0378021 | 95.690 | 116 | 4 | 1 | 1 | 115 | 1 | 116 | 9.80e-79 | 227 |
| Msa1389900 | MtrunA17_Chr4g0067331 | 37.500 | 120 | 53 | 5 | 14 | 115 | 10 | 125 | 3.55e-14 | 63.9 |
| Msa1389900 | MtrunA17_Chr7g0269131 | 35.294 | 119 | 62 | 4 | 10 | 117 | 4 | 118 | 1.45e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1389900 | AT4G14010.1 | 46.087 | 115 | 47 | 5 | 10 | 118 | 12 | 117 | 1.05e-25 | 93.6 |
| Msa1389900 | AT1G02900.1 | 38.400 | 125 | 63 | 4 | 1 | 116 | 1 | 120 | 4.00e-15 | 66.6 |
| Msa1389900 | AT4G15800.1 | 46.341 | 82 | 37 | 3 | 37 | 115 | 38 | 115 | 3.39e-13 | 61.6 |
Find 20 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTAGAACATCCTCTATTATA+AGG | 0.298490 | tig0021306:+10229 | None:intergenic |
| GTTCTAAGTATTATCGTTGT+AGG | 0.352031 | tig0021306:-10213 | Msa1389900:CDS |
| TCAAGAAGATGAACAATTGA+TGG | 0.375070 | tig0021306:-10395 | Msa1389900:CDS |
| CCTCTATTATAAGGATTTGA+AGG | 0.425611 | tig0021306:+10238 | None:intergenic |
| GCATGAAGTAGAAGAAGGTC+AGG | 0.441178 | tig0021306:+10509 | None:intergenic |
| CCTGCACCACCATTACAAAC+TGG | 0.451169 | tig0021306:+10301 | None:intergenic |
| TTCTTGATTGCATTCAGCTA+TGG | 0.463317 | tig0021306:+10411 | None:intergenic |
| GAAGCTTATAGTAAATCTGC+AGG | 0.482571 | tig0021306:-10274 | Msa1389900:CDS |
| CCTTCAAATCCTTATAATAG+AGG | 0.483889 | tig0021306:-10238 | Msa1389900:CDS |
| CCAGTTTGTAATGGTGGTGC+AGG | 0.525366 | tig0021306:-10301 | Msa1389900:CDS |
| GATAAACCAGTTTGTAATGG+TGG | 0.525715 | tig0021306:-10307 | Msa1389900:CDS |
| AGAGATAAACCAGTTTGTAA+TGG | 0.535103 | tig0021306:-10310 | Msa1389900:CDS |
| TTATAAGGATTTGAAGGTGG+AGG | 0.542122 | tig0021306:+10244 | None:intergenic |
| GAAGAGCATGAAGTAGAAGA+AGG | 0.591507 | tig0021306:+10504 | None:intergenic |
| CAAAGGAGATACATTTCTGA+AGG | 0.595659 | tig0021306:-10343 | Msa1389900:CDS |
| CTATTATAAGGATTTGAAGG+TGG | 0.614457 | tig0021306:+10241 | None:intergenic |
| CATCATGCAAGCATATGCAA+TGG | 0.634173 | tig0021306:-10436 | Msa1389900:CDS |
| TGGAGTCAGAAATAAGCAGA+AGG | 0.637756 | tig0021306:-10375 | Msa1389900:CDS |
| GTTTGTAATGGTGGTGCAGG+TGG | 0.645633 | tig0021306:-10298 | Msa1389900:CDS |
| AAGCTTATAGTAAATCTGCA+GGG | 0.695265 | tig0021306:-10273 | Msa1389900:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTAGAACATCCTCTATTATA+AGG | + | tig0021306:10510-10529 | None:intergenic | 25.0% |
| TCAAGAAGATGAACAATTGA+TGG | - | tig0021306:10341-10360 | Msa1389900:CDS | 30.0% | |
| ! | AGAAATAAGCAGAAGGTTTT+TGG | - | tig0021306:10368-10387 | Msa1389900:CDS | 30.0% |
| AGAGATAAACCAGTTTGTAA+TGG | - | tig0021306:10426-10445 | Msa1389900:CDS | 30.0% | |
| AAGCTTATAGTAAATCTGCA+GGG | - | tig0021306:10463-10482 | Msa1389900:CDS | 30.0% | |
| CTATTATAAGGATTTGAAGG+TGG | + | tig0021306:10498-10517 | None:intergenic | 30.0% | |
| CCTCTATTATAAGGATTTGA+AGG | + | tig0021306:10501-10520 | None:intergenic | 30.0% | |
| CCTTCAAATCCTTATAATAG+AGG | - | tig0021306:10498-10517 | Msa1389900:CDS | 30.0% | |
| ! | GTTCTAAGTATTATCGTTGT+AGG | - | tig0021306:10523-10542 | Msa1389900:CDS | 30.0% |
| TTCTTGATTGCATTCAGCTA+TGG | + | tig0021306:10328-10347 | None:intergenic | 35.0% | |
| CAAAGGAGATACATTTCTGA+AGG | - | tig0021306:10393-10412 | Msa1389900:CDS | 35.0% | |
| GATAAACCAGTTTGTAATGG+TGG | - | tig0021306:10429-10448 | Msa1389900:CDS | 35.0% | |
| GAAGCTTATAGTAAATCTGC+AGG | - | tig0021306:10462-10481 | Msa1389900:CDS | 35.0% | |
| TTATAAGGATTTGAAGGTGG+AGG | + | tig0021306:10495-10514 | None:intergenic | 35.0% | |
| ! | GAAGAGCATGAAGTAGAAGA+AGG | + | tig0021306:10235-10254 | None:intergenic | 40.0% |
| CATCATGCAAGCATATGCAA+TGG | - | tig0021306:10300-10319 | Msa1389900:CDS | 40.0% | |
| TGGAGTCAGAAATAAGCAGA+AGG | - | tig0021306:10361-10380 | Msa1389900:CDS | 40.0% | |
| GCATGAAGTAGAAGAAGGTC+AGG | + | tig0021306:10230-10249 | None:intergenic | 45.0% | |
| !!! | GCAGAAGGTTTTTGGAGCAA+AGG | - | tig0021306:10376-10395 | Msa1389900:CDS | 45.0% |
| CCTGCACCACCATTACAAAC+TGG | + | tig0021306:10438-10457 | None:intergenic | 50.0% | |
| ! | CCAGTTTGTAATGGTGGTGC+AGG | - | tig0021306:10435-10454 | Msa1389900:CDS | 50.0% |
| ! | GTTTGTAATGGTGGTGCAGG+TGG | - | tig0021306:10438-10457 | Msa1389900:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0021306 | gene | 10201 | 10557 | 10201 | ID=Msa1389900;Name=Msa1389900 |
| tig0021306 | mRNA | 10201 | 10557 | 10201 | ID=Msa1389900-mRNA-1;Parent=Msa1389900;Name=Msa1389900-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|-1|0|1|-1|1|1|0|118 |
| tig0021306 | exon | 10201 | 10557 | 10201 | ID=Msa1389900-mRNA-1:exon:11919;Parent=Msa1389900-mRNA-1 |
| tig0021306 | CDS | 10201 | 10557 | 10201 | ID=Msa1389900-mRNA-1:cds;Parent=Msa1389900-mRNA-1 |
| Gene Sequence |
| Protein sequence |
