AlfalfaGEDB | Msa1450310

M. sativa cultivar zhongmu-4 / Msa1450310

KEGG: -
GO: GO:0000118 GO:0000122 GO:0000228 GO:0000785 GO:0000790 GO:0001085 GO:0001103 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0006355 GO:0006357 GO:0008134 GO:0008150 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0015630 GO:0016043 GO:0016581 GO:0017053 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032991 GO:0032993 GO:0042826 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0045892 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050808 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0060255 GO:0065007 GO:0070013 GO:0070491 GO:0070603 GO:0071840 GO:0072553 GO:0080090 GO:0090545 GO:0090568 GO:0099172 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1904949 GO:2000112 GO:2000113 GO:2001141
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1450310	sp\|F4IXE7\|IDM1_ARATH	33.168	202	126	4	252	445	833	1033	2.52e-24	110

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1450310	A0A072VBX5	91.741	448	18	2	1	448	601	1029	0.0	838

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0024110	Msa1450310	0.816782	4.630965e-52	-8.615850e-47
Msa0038000	Msa1450310	0.829845	4.098540e-55	-8.615850e-47
Msa0038440	Msa1450310	0.804333	2.271465e-49	-8.615850e-47
Msa0070430	Msa1450310	0.828703	7.756843e-55	-8.615850e-47
Msa0097950	Msa1450310	0.817478	3.229478e-52	-8.615850e-47
Msa0108870	Msa1450310	0.828231	1.008271e-54	-8.615850e-47
Msa1380900	Msa1450310	0.802195	6.295950e-49	-8.615850e-47
Msa1389850	Msa1450310	0.809757	1.616299e-50	-8.615850e-47
Msa1396870	Msa1450310	0.806117	9.613358e-50	-8.615850e-47
Msa1401950	Msa1450310	0.821458	3.995058e-53	-8.615850e-47
Msa1411950	Msa1450310	0.807127	5.883612e-50	-8.615850e-47
Msa1416260	Msa1450310	0.818676	1.731086e-52	-8.615850e-47
Msa1435770	Msa1450310	0.827779	1.295417e-54	-8.615850e-47
Msa0619920	Msa1450310	0.802212	6.243570e-49	-8.615850e-47
Msa0620230	Msa1450310	0.812429	4.258586e-51	-8.615850e-47
Msa0624680	Msa1450310	0.800424	1.450747e-48	-8.615850e-47
Msa0628420	Msa1450310	0.808901	2.465798e-50	-8.615850e-47
Msa0652300	Msa1450310	0.805739	1.154290e-49	-8.615850e-47
Msa0656960	Msa1450310	0.828835	7.208924e-55	-8.615850e-47
Msa0679100	Msa1450310	0.829234	5.770560e-55	-8.615850e-47
Msa0690310	Msa1450310	0.814707	1.342964e-51	-8.615850e-47
Msa0743530	Msa1450310	0.827578	1.448145e-54	-8.615850e-47
Msa0744100	Msa1450310	0.805517	1.284809e-49	-8.615850e-47
Msa0786750	Msa1450310	0.805542	1.269273e-49	-8.615850e-47
Msa0822170	Msa1450310	0.829642	4.591877e-55	-8.615850e-47
Msa0822180	Msa1450310	0.818603	1.798426e-52	-8.615850e-47
Msa0825270	Msa1450310	0.803260	3.794434e-49	-8.615850e-47
Msa0828680	Msa1450310	0.810260	1.259056e-50	-8.615850e-47
Msa0829220	Msa1450310	0.827175	1.808957e-54	-8.615850e-47
Msa0136120	Msa1450310	0.810759	9.824721e-51	-8.615850e-47
Msa0138360	Msa1450310	0.804555	2.042211e-49	-8.615850e-47
Msa0147980	Msa1450310	0.823978	1.035028e-53	-8.615850e-47
Msa0150260	Msa1450310	0.802060	6.710886e-49	-8.615850e-47
Msa0165440	Msa1450310	0.800809	1.210605e-48	-8.615850e-47
Msa0171850	Msa1450310	0.801006	1.103751e-48	-8.615850e-47
Msa0175540	Msa1450310	0.801701	7.953951e-49	-8.615850e-47
Msa0198520	Msa1450310	0.810640	1.042492e-50	-8.615850e-47
Msa0198530	Msa1450310	0.814073	1.854717e-51	-8.615850e-47
Msa0221600	Msa1450310	0.819192	1.321338e-52	-8.615850e-47
Msa0237200	Msa1450310	0.807869	4.094865e-50	-8.615850e-47
Msa1227460	Msa1450310	0.803347	3.640418e-49	-8.615850e-47
Msa1230040	Msa1450310	0.817325	3.497276e-52	-8.615850e-47
Msa1259640	Msa1450310	0.803673	3.115456e-49	-8.615850e-47
Msa1270420	Msa1450310	0.802755	4.825620e-49	-8.615850e-47
Msa1272600	Msa1450310	0.815662	8.241241e-52	-8.615850e-47
Msa1310450	Msa1450310	0.800913	1.153223e-48	-8.615850e-47
Msa1331350	Msa1450310	0.817605	3.023189e-52	-8.615850e-47
Msa1344310	Msa1450310	0.809811	1.573576e-50	-8.615850e-47
Msa0384670	Msa1450310	0.809818	1.567698e-50	-8.615850e-47
Msa0394690	Msa1450310	0.821776	3.373779e-53	-8.615850e-47
Msa0432500	Msa1450310	0.819512	1.116977e-52	-8.615850e-47
Msa0434570	Msa1450310	0.815655	8.273249e-52	-8.615850e-47
Msa0434770	Msa1450310	0.816490	5.384925e-52	-8.615850e-47
Msa0478580	Msa1450310	0.834172	3.495064e-56	-8.615850e-47
Msa1010460	Msa1450310	0.813525	2.449526e-51	-8.615850e-47
Msa1068100	Msa1450310	0.808478	3.036557e-50	-8.615850e-47
Msa1075970	Msa1450310	0.803532	3.332595e-49	-8.615850e-47
Msa0316940	Msa1450310	0.815348	9.678163e-52	-8.615850e-47
Msa0324240	Msa1450310	0.805249	1.462388e-49	-8.615850e-47
Msa0342290	Msa1450310	0.833991	3.879649e-56	-8.615850e-47
Msa1113590	Msa1450310	0.800410	1.460488e-48	-8.615850e-47
Msa1128330	Msa1450310	0.837002	6.718595e-57	-8.615850e-47
Msa1206650	Msa1450310	0.819813	9.534880e-53	-8.615850e-47
Msa1211340	Msa1450310	0.802989	4.316811e-49	-8.615850e-47
Msa1212730	Msa1450310	0.807792	4.251802e-50	-8.615850e-47
Msa0509120	Msa1450310	0.810521	1.106049e-50	-8.615850e-47
Msa0515710	Msa1450310	0.800021	1.752235e-48	-8.615850e-47
Msa0584550	Msa1450310	0.820307	7.349631e-53	-8.615850e-47
Msa0880450	Msa1450310	0.817738	2.822535e-52	-8.615850e-47
Msa0906690	Msa1450310	0.815871	7.402613e-52	-8.615850e-47
Msa0928120	Msa1450310	0.800357	1.496877e-48	-8.615850e-47
Msa0939000	Msa1450310	0.810530	1.101209e-50	-8.615850e-47
Msa0972590	Msa1450310	0.803249	3.814764e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1450310	MtrunA17_Chr2g0320451	91.741	448	18	2	1	448	606	1034	0.0	838
Msa1450310	MtrunA17_Chr7g0265211	55.497	191	76	2	249	438	766	948	1.11e-63	221
Msa1450310	MtrunA17_Chr7g0265211	58.537	123	50	1	8	129	644	766	4.59e-44	165
Msa1450310	MtrunA17_Chr1g0182481	54.211	190	77	4	249	437	713	893	1.24e-57	204
Msa1450310	MtrunA17_Chr1g0182481	47.059	102	53	1	29	129	612	713	4.11e-23	102
Msa1450310	MtrunA17_Chr1g0179841	51.667	180	78	2	260	438	632	803	4.99e-57	201
Msa1450310	MtrunA17_Chr1g0179841	59.690	129	51	1	2	129	493	621	2.19e-44	166
Msa1450310	MtrunA17_Chr4g0025251	53.403	191	81	4	249	437	517	701	6.07e-55	194
Msa1450310	MtrunA17_Chr4g0025251	34.872	195	86	4	2	159	353	543	4.02e-24	105
Msa1450310	MtrunA17_Chr4g0028111	54.217	166	69	3	244	408	400	559	4.13e-48	174
Msa1450310	MtrunA17_Chr4g0028111	45.833	144	75	2	2	144	277	418	6.00e-26	110
Msa1450310	MtrunA17_Chr4g0027811	41.758	182	65	3	260	440	441	582	2.61e-33	132
Msa1450310	MtrunA17_Chr4g0027811	50.000	116	57	1	2	116	323	438	9.12e-26	110
Msa1450310	MtrunA17_Chr3g0105291	32.275	189	114	4	266	442	827	1013	1.39e-23	104
Msa1450310	MtrunA17_Chr5g0430361	30.870	230	138	5	227	442	840	1062	1.84e-23	103
Msa1450310	MtrunA17_Chr1g0205451	36.424	151	93	2	287	435	861	1010	9.95e-23	101
Msa1450310	MtrunA17_Chr2g0316251	36.601	153	94	2	287	437	1015	1166	1.27e-20	95.1
Msa1450310	MtrunA17_Chr3g0134821	27.468	233	142	6	237	443	609	840	3.31e-19	90.5
Msa1450310	MtrunA17_Chr1g0185491	31.325	166	106	4	276	437	1059	1220	5.97e-19	90.1
Msa1450310	MtrunA17_Chr5g0407251	29.050	179	120	4	266	439	245	421	2.82e-18	86.7
Msa1450310	MtrunA17_Chr3g0134811	30.556	180	114	4	266	437	619	795	2.85e-18	87.8
Msa1450310	MtrunA17_Chr7g0270101	28.409	176	111	4	266	426	607	782	8.77e-18	86.3
Msa1450310	MtrunA17_Chr5g0407211	28.492	179	121	4	266	439	245	421	1.17e-17	84.7
Msa1450310	MtrunA17_Chr7g0259981	29.375	160	98	4	290	440	142	295	5.48e-15	75.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1450310	AT2G27980.1	57.778	180	69	1	259	438	886	1058	1.20e-67	233
Msa1450310	AT2G27980.1	52.459	122	56	2	8	127	750	871	6.12e-35	139
Msa1450310	AT2G37520.1	54.737	190	77	3	249	438	636	816	2.41e-62	216
Msa1450310	AT2G37520.1	50.000	100	49	1	29	127	535	634	3.52e-26	112
Msa1450310	AT3G53680.1	51.515	198	87	3	243	440	646	834	9.35e-61	212
Msa1450310	AT3G53680.1	45.455	110	58	2	21	128	542	651	1.28e-23	104
Msa1450310	AT3G53680.2	51.515	198	87	3	243	440	648	836	1.01e-60	212
Msa1450310	AT3G53680.2	45.455	110	58	2	21	128	544	653	1.41e-23	104
Msa1450310	AT2G36720.1	50.952	210	92	3	229	437	774	973	3.59e-59	209
Msa1450310	AT2G36720.1	51.220	123	59	1	8	129	670	792	6.57e-37	144
Msa1450310	AT2G36720.2	50.952	210	92	3	229	437	774	973	3.59e-59	209
Msa1450310	AT2G36720.2	51.220	123	59	1	8	129	670	792	6.57e-37	144
Msa1450310	AT3G14980.6	33.168	202	126	4	252	445	833	1033	2.57e-25	110
Msa1450310	AT3G14980.1	33.168	202	126	4	252	445	833	1033	2.57e-25	110
Msa1450310	AT3G14980.2	33.168	202	126	4	252	445	833	1033	2.57e-25	110
Msa1450310	AT3G14980.5	33.168	202	126	4	252	445	788	988	2.69e-25	110
Msa1450310	AT1G05380.3	33.508	191	121	3	252	437	736	925	2.81e-25	109
Msa1450310	AT1G05380.1	33.508	191	121	3	252	437	736	925	2.81e-25	109
Msa1450310	AT1G05380.2	33.508	191	121	3	252	437	736	925	2.81e-25	109
Msa1450310	AT3G14980.3	35.556	180	111	3	270	445	855	1033	4.20e-25	109
Msa1450310	AT5G58610.4	29.187	209	127	4	254	442	584	791	3.08e-22	100
Msa1450310	AT4G14920.3	33.775	151	97	2	287	435	832	981	4.61e-22	100
Msa1450310	AT4G14920.2	33.775	151	97	2	287	435	832	981	4.82e-22	100
Msa1450310	AT4G14920.1	33.775	151	97	2	287	435	832	981	4.82e-22	100
Msa1450310	AT5G58610.3	29.187	209	127	4	254	442	815	1022	5.20e-22	99.8
Msa1450310	AT5G58610.5	29.187	209	127	4	254	442	806	1013	7.17e-22	99.4
Msa1450310	AT5G58610.8	32.335	167	102	2	266	421	827	993	7.57e-22	99.4
Msa1450310	AT5G58610.2	32.335	167	102	2	266	421	827	993	7.57e-22	99.4
Msa1450310	AT5G58610.7	29.187	209	127	4	254	442	806	1013	7.59e-22	99.4
Msa1450310	AT5G58610.1	30.952	168	104	3	286	442	821	987	2.53e-20	94.7
Msa1450310	AT5G58610.6	30.952	168	104	3	286	442	821	987	2.68e-20	94.7
Msa1450310	AT5G36740.5	31.373	153	102	2	283	433	794	945	1.14e-18	89.7
Msa1450310	AT5G36740.1	31.373	153	102	2	283	433	794	945	1.14e-18	89.7
Msa1450310	AT5G36740.4	31.373	153	102	2	283	433	651	802	1.67e-18	89.0
Msa1450310	AT5G63900.1	31.461	178	115	4	253	430	363	533	5.23e-18	87.0
Msa1450310	AT5G58610.10	27.692	130	83	2	266	384	827	956	3.26e-11	66.2
Msa1450310	AT5G58610.9	26.761	142	93	2	254	384	806	947	4.46e-11	65.9

Find 132 sgRNAs with CRISPR-Local

Find 195 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCAATATGGACTAACAAATT+TGG	0.126413	tig0032878:+3381	Msa1450310:CDS
CTAGTGAATTTGGTTGCTTC+TGG	0.137104	tig0032878:+2058	Msa1450310:CDS
TCATTCTTCTCTAGTGAATT+TGG	0.143744	tig0032878:+2048	Msa1450310:CDS
CATGATTTCGTTAAAATATT+TGG	0.183205	tig0032878:+1714	Msa1450310:CDS
TTATACAATGGCCTTTGTTT+TGG	0.187927	tig0032878:+3629	Msa1450310:three_prime_UTR
AAGCATGACACTGGCGTTAA+AGG	0.217277	tig0032878:+2124	Msa1450310:CDS
CCTATAGTTGATTCGTCTTC+TGG	0.255755	tig0032878:+2794	Msa1450310:CDS
CATGCATTCCCAGTGATTCT+TGG	0.281125	tig0032878:+1293	Msa1450310:five_prime_UTR
CAATATGGACTAACAAATTT+GGG	0.291708	tig0032878:+3382	Msa1450310:CDS
AACAAAGGCCATTGTATAAG+AGG	0.319583	tig0032878:-3625	None:intergenic
AGCCAAGTTGTTGTTTCTGC+TGG	0.322586	tig0032878:+3087	Msa1450310:intron
AGAACCCTCCATTTAATTTC+AGG	0.332016	tig0032878:-2520	None:intergenic
ATCAAACTTAGAATAGAATC+TGG	0.336604	tig0032878:-2094	None:intergenic
GAAGAGAAAAGTGTAGAAAC+TGG	0.337799	tig0032878:+2463	Msa1450310:CDS
GCACAACATGCAGAATTTAA+AGG	0.342742	tig0032878:+1904	Msa1450310:CDS
TTTGTAGCAAAGAAATGATA+TGG	0.344180	tig0032878:+3757	Msa1450310:three_prime_UTR
TAGTGAATTTGGTTGCTTCT+GGG	0.344854	tig0032878:+2059	Msa1450310:CDS
GTGTTGATCCTGAAATTAAA+TGG	0.365058	tig0032878:+2512	Msa1450310:CDS
GACACTACCAAGTACTTATC+AGG	0.365241	tig0032878:+3578	Msa1450310:CDS
TGTCTACAGCTTTCTCTTCA+AGG	0.369525	tig0032878:-2324	None:intergenic
TTCATCCCGACAATGTTGTT+TGG	0.370169	tig0032878:+2824	Msa1450310:CDS
TCATCCCGACAATGTTGTTT+GGG	0.376162	tig0032878:+2825	Msa1450310:CDS
ACATAATTTGTTGCACCCTT+TGG	0.377601	tig0032878:+3949	Msa1450310:three_prime_UTR
CCGATGAAGCTGAATCAATA+TGG	0.379351	tig0032878:+3367	Msa1450310:CDS
AACAGTAATTATTTGTGATC+AGG	0.388685	tig0032878:+1743	Msa1450310:CDS
GTTGTGCATTACTCTCCATT+TGG	0.390065	tig0032878:-1342	None:intergenic
TCTCCATTTGGAAATTAGTC+TGG	0.397428	tig0032878:-1330	None:intergenic
AAAGGAAGTTCTGCGACTTC+TGG	0.407739	tig0032878:-3129	None:intergenic
CTGCAGAACCAATCCCCTTC+AGG	0.408802	tig0032878:-2007	None:intergenic
AGCATGACACTGGCGTTAAA+GGG	0.412172	tig0032878:+2125	Msa1450310:CDS
AGGAGTTGCCTGAAGGGGAT+TGG	0.413414	tig0032878:+1999	Msa1450310:intron
TTAAATGGAGGGTTCTGAAC+TGG	0.416023	tig0032878:+2527	Msa1450310:CDS
CGTCGGTTTATCTGTTCTAA+AGG	0.422199	tig0032878:-1409	None:intergenic
CTGGCAGCTGGAAGGATTGA+AGG	0.437216	tig0032878:+1379	Msa1450310:CDS
AGCAATTGTCTGATGAATAT+AGG	0.441070	tig0032878:+2575	Msa1450310:CDS
GTTTGATATATAATATCTAA+AGG	0.445999	tig0032878:+4000	Msa1450310:three_prime_UTR
TATCATATGATGATCTTTCA+AGG	0.446445	tig0032878:+3528	Msa1450310:CDS
TTAGAGGCCCTCTTATACAA+TGG	0.447122	tig0032878:+3617	Msa1450310:three_prime_UTR
AGAAGTCGCAGAACTTCCTT+TGG	0.454166	tig0032878:+3131	Msa1450310:CDS
TTTGATATATAATATCTAAA+GGG	0.455220	tig0032878:+4001	Msa1450310:three_prime_UTR
TTTACAATACCAAGAATCAC+TGG	0.461073	tig0032878:-1302	None:intergenic
AATGCGAATGCTCTGGCAGC+TGG	0.469473	tig0032878:+1367	Msa1450310:CDS
GGTGCAGACACTGGCGTTGA+AGG	0.470080	tig0032878:+2151	Msa1450310:CDS
TGCAGCTTTCTCTTCAACGC+CGG	0.470611	tig0032878:-2374	None:intergenic
AGTGAATTTGGTTGCTTCTG+GGG	0.474324	tig0032878:+2060	Msa1450310:CDS
AACTGGAAGTTAGTTGCCTC+AGG	0.474897	tig0032878:+2544	Msa1450310:CDS
CCAGAAGACGAATCAACTAT+AGG	0.477562	tig0032878:-2794	None:intergenic
CTTACCCAAACAACATTGTC+GGG	0.482749	tig0032878:-2829	None:intergenic
GTACTTGGTAGTGTCGGAAC+AGG	0.484591	tig0032878:-3570	None:intergenic
TCCACCTTTCTCTTCGACGC+CGG	0.484681	tig0032878:-2215	None:intergenic
ATATCATGTTGGGTGTTTGA+AGG	0.500876	tig0032878:+1880	Msa1450310:CDS
TCACAAATAATTACTGTTCG+AGG	0.501951	tig0032878:-1738	None:intergenic
AAAGTGTAGCTGTCGACCAT+GGG	0.503995	tig0032878:+1449	Msa1450310:CDS
TTGCCAGACTAATTTCCAAA+TGG	0.504901	tig0032878:+1327	Msa1450310:exon
GGCGTTGAAGGGAAAGGTGC+AGG	0.507254	tig0032878:+2163	Msa1450310:CDS
AATAGAGCATTACCCACATA+AGG	0.507350	tig0032878:-1479	None:intergenic
TAAATTAAATAACCTGTCCT+TGG	0.508226	tig0032878:-3173	None:intergenic
CCATATTGATTCAGCTTCAT+CGG	0.513940	tig0032878:-3367	None:intergenic
AAATATACTCACATGGAAGA+TGG	0.518050	tig0032878:-2620	None:intergenic
AGTCGCAGAACTTCCTTTGG+TGG	0.525155	tig0032878:+3134	Msa1450310:CDS
TACAAGATCAAAATACCAAA+GGG	0.527683	tig0032878:-3964	None:intergenic
CGTGTTCTTGCAGTGTGAGA+AGG	0.527948	tig0032878:+1856	Msa1450310:intron
TACAGCTTTCTCTTCAACGC+CGG	0.528983	tig0032878:-2347	None:intergenic
CATCGGCAGCCGGTAGGACA+AGG	0.533571	tig0032878:-3350	None:intergenic
CTCCAGCAGAAACAACAACT+TGG	0.536328	tig0032878:-3089	None:intergenic
ACAACGTAATGCGAATGCTC+TGG	0.538110	tig0032878:+1360	Msa1450310:CDS
GATTCAGCTTCATCGGCAGC+CGG	0.539170	tig0032878:-3360	None:intergenic
ATCATATGATGATCTTTCAA+GGG	0.539202	tig0032878:+3529	Msa1450310:CDS
AATGTCATTGAAGAGAAAGT+TGG	0.540071	tig0032878:+2187	Msa1450310:CDS
GCTTACCCAAACAACATTGT+CGG	0.540429	tig0032878:-2830	None:intergenic
ACAATCTCCTGATAAGTACT+TGG	0.540795	tig0032878:-3585	None:intergenic
GAAACTGGGGTTGAAGAGAA+AGG	0.541450	tig0032878:+2478	Msa1450310:CDS
AGAGACACTGTTGAAGAGAA+AGG	0.541782	tig0032878:+2262	Msa1450310:CDS
CAAGAATCACTGGGAATGCA+TGG	0.541894	tig0032878:-1292	None:intergenic
TCAAACTTAGAATAGAATCT+GGG	0.542276	tig0032878:-2093	None:intergenic
GACACTGGCGTTGAAGGGAA+AGG	0.543459	tig0032878:+2157	Msa1450310:CDS
AAGAGAAAAGTGTAGAAACT+GGG	0.543844	tig0032878:+2464	Msa1450310:CDS
AAAAGTGTAGCTGTCGACCA+TGG	0.546066	tig0032878:+1448	Msa1450310:CDS
GACACTGGCGTCGAAGAGAA+AGG	0.550272	tig0032878:+2238	Msa1450310:CDS
GTGCAGACACTGGCGTTGAA+GGG	0.555836	tig0032878:+2152	Msa1450310:CDS
TGACAATACGAATGCATCGT+CGG	0.558080	tig0032878:-1426	None:intergenic
GAAGAGAAAGCTGCAGACAC+TGG	0.558710	tig0032878:+2382	Msa1450310:CDS
CTGACTAGGACATGGGAGAA+GGG	0.558824	tig0032878:+3812	Msa1450310:three_prime_UTR
CGAATGCTCTGGCAGCTGGA+AGG	0.559415	tig0032878:+1371	Msa1450310:CDS
GAAGAGAAAGGTGTAGTCAC+TGG	0.559508	tig0032878:+2490	Msa1450310:CDS
ACTGACTAGGACATGGGAGA+AGG	0.564172	tig0032878:+3811	Msa1450310:three_prime_UTR
GACACCGGCGTCGAAGAGAA+AGG	0.567801	tig0032878:+2211	Msa1450310:CDS
GAAGAGAAAGGTGGAGACAC+TGG	0.570991	tig0032878:+2223	Msa1450310:CDS
GAAGAGAAAGGTGGAGACAC+AGG	0.586297	tig0032878:+2274	Msa1450310:CDS
TATTGCATCATCAGCAAAGG+TGG	0.589273	tig0032878:-3732	None:intergenic
AGTTTCTGAGTACCAAGGAC+AGG	0.590743	tig0032878:+3161	Msa1450310:CDS
TCAGAAACTGTCGCCACCAA+AGG	0.590813	tig0032878:-3147	None:intergenic
AAAGGGAAAGGTGCAGACAC+TGG	0.591411	tig0032878:+2142	Msa1450310:CDS
GAGGAAACTACTAACTGACT+AGG	0.592540	tig0032878:+3798	Msa1450310:three_prime_UTR
TTACAAGATCAAAATACCAA+AGG	0.592739	tig0032878:-3965	None:intergenic
TGATCCTGAAATTAAATGGA+GGG	0.593172	tig0032878:+2516	Msa1450310:CDS
TTTCCAGGAGTTGCCTGAAG+GGG	0.593788	tig0032878:+1994	Msa1450310:intron
ACAAAGGCCATTGTATAAGA+GGG	0.594859	tig0032878:-3624	None:intergenic
TTGATCCTGAAATTAAATGG+AGG	0.600007	tig0032878:+2515	Msa1450310:CDS
ATCGCATTAAAACCGCTCGA+TGG	0.600094	tig0032878:-3886	None:intergenic
GTGAGAAGGAATATCATGTT+GGG	0.603127	tig0032878:+1870	Msa1450310:CDS
GACACTGGCGTTAAAGGGAA+AGG	0.604412	tig0032878:+2130	Msa1450310:CDS
CTACTAACTGACTAGGACAT+GGG	0.606322	tig0032878:+3805	Msa1450310:three_prime_UTR
CAGCTTCATCGGCAGCCGGT+AGG	0.610119	tig0032878:-3356	None:intergenic
TCACTGGGAATGCATGGCAG+AGG	0.610288	tig0032878:-1286	None:intergenic
ACTACTAACTGACTAGGACA+TGG	0.611876	tig0032878:+3804	Msa1450310:three_prime_UTR
ACTTATAAGCTGCCATCGAG+CGG	0.612029	tig0032878:+3874	Msa1450310:three_prime_UTR
ACATTGTCGGGATGAAATCA+CGG	0.613680	tig0032878:-2817	None:intergenic
TGTGAGAAGGAATATCATGT+TGG	0.614659	tig0032878:+1869	Msa1450310:CDS
AAATATTGCATCATCAGCAA+AGG	0.615533	tig0032878:-3735	None:intergenic
GAAGAGAAAGTTGCAGACAC+TGG	0.615896	tig0032878:+2301	Msa1450310:CDS
TGATAAGTACTTGGTAGTGT+CGG	0.620438	tig0032878:-3576	None:intergenic
TCAAAACAAACCCAAAACAA+AGG	0.622758	tig0032878:-3640	None:intergenic
GACACTGTTGAAGAGAAAGG+TGG	0.630766	tig0032878:+2265	Msa1450310:CDS
TTCCAGCAAAATCTTGCCCG+CGG	0.645881	tig0032878:-2953	None:intergenic
GAAGAGAAAGTTGGAGACAC+CGG	0.647088	tig0032878:+2196	Msa1450310:CDS
AAGTGTAGCTGTCGACCATG+GGG	0.651411	tig0032878:+1450	Msa1450310:CDS
GAAGAGAAATGTGGAGACAC+TGG	0.657663	tig0032878:+2409	Msa1450310:CDS
ACCGGCGTCGAAGAGAAAGG+TGG	0.661032	tig0032878:+2214	Msa1450310:CDS
TACAAAGCTCGAGCACGATG+AGG	0.664765	tig0032878:+3407	Msa1450310:CDS
AGAGAAAAGTGTAGAAACTG+GGG	0.664950	tig0032878:+2465	Msa1450310:CDS
ATATAGGCAAGTGCTTTCGA+AGG	0.665401	tig0032878:+2591	Msa1450310:CDS
GAAGAGAAAGCTGTAGACAC+CGG	0.666714	tig0032878:+2328	Msa1450310:CDS
ACTGGCGTTGAAGAGAAATG+TGG	0.671366	tig0032878:+2400	Msa1450310:CDS
TTACAATACCAAGAATCACT+GGG	0.673631	tig0032878:-1301	None:intergenic
GAAGAGAAAGCTGTAGACAC+CGG	0.678790	tig0032878:+2355	Msa1450310:CDS
AACAGGCCTCTGTAGTACTG+AGG	0.693954	tig0032878:-3553	None:intergenic
AAGGGACCTCAGTACTACAG+AGG	0.712714	tig0032878:+3547	Msa1450310:CDS
AGTGTAGCTGTCGACCATGG+GGG	0.718999	tig0032878:+1451	Msa1450310:CDS
GCGACAGTTTCTGAGTACCA+AGG	0.724330	tig0032878:+3156	Msa1450310:CDS
GTTGAGAAAGATGTAGACAC+TGG	0.734524	tig0032878:+2436	Msa1450310:CDS
GAGAAATGTGGAGACACTGG+TGG	0.757461	tig0032878:+2412	Msa1450310:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAGCAAATTAAAAAAATAGA+AGG	-	tig0032878:1642-1661	None:intergenic	15.0%
!!	TAAAAGTAGAAAAGAGAAAT+AGG	-	tig0032878:1547-1566	None:intergenic	20.0%
!!	CATGATTTCGTTAAAATATT+TGG	+	tig0032878:1714-1733	Msa1450310:CDS	20.0%
!!	GAAAACAAATAAAAAGAAAC+TGG	-	tig0032878:3067-3086	None:intergenic	20.0%
!!	ATTCTTTAACTCAATTATAC+TGG	+	tig0032878:3256-3275	Msa1450310:intron	20.0%
!!	GAGATTAGAATTAAAAATTG+AGG	+	tig0032878:3779-3798	Msa1450310:three_prime_UTR	20.0%
!!	AATTTGTATCAACAAAAATC+TGG	-	tig0032878:3853-3872	None:intergenic	20.0%
!	AACAGTAATTATTTGTGATC+AGG	+	tig0032878:1743-1762	Msa1450310:CDS	25.0%
!	TAAAAGCAGTTGACAAAAAA+GGG	-	tig0032878:1799-1818	None:intergenic	25.0%
!	ATAAAAGCAGTTGACAAAAA+AGG	-	tig0032878:1800-1819	None:intergenic	25.0%
!	TCAAACTTAGAATAGAATCT+GGG	-	tig0032878:2096-2115	None:intergenic	25.0%
!	ATCAAACTTAGAATAGAATC+TGG	-	tig0032878:2097-2116	None:intergenic	25.0%
!	GAAACATAAATATACTCACA+TGG	-	tig0032878:2630-2649	None:intergenic	25.0%
!!!	ATTTGTTGTTGTAACGATTA+TGG	+	tig0032878:2878-2897	Msa1450310:intron	25.0%
!	AATAAAAAGAAACTGGAATC+AGG	-	tig0032878:3060-3079	None:intergenic	25.0%
!	TAAATTAAATAACCTGTCCT+TGG	-	tig0032878:3176-3195	None:intergenic	25.0%
!!!	TCAATTATACTGGTTTATTC+AGG	+	tig0032878:3266-3285	Msa1450310:intron	25.0%
!!!	CAATTATACTGGTTTATTCA+GGG	+	tig0032878:3267-3286	Msa1450310:intron	25.0%
!!	TCAATATGGACTAACAAATT+TGG	+	tig0032878:3381-3400	Msa1450310:CDS	25.0%
!!	CAATATGGACTAACAAATTT+GGG	+	tig0032878:3382-3401	Msa1450310:CDS	25.0%
!	GAGTAGTAAACAATATAACT+AGG	-	tig0032878:3455-3474	None:intergenic	25.0%
!	TATCATATGATGATCTTTCA+AGG	+	tig0032878:3528-3547	Msa1450310:CDS	25.0%
!	ATCATATGATGATCTTTCAA+GGG	+	tig0032878:3529-3548	Msa1450310:CDS	25.0%
!!!	AGATTGTGTAAAGTTTTTAG+AGG	+	tig0032878:3601-3620	Msa1450310:exon	25.0%
!	TTTGTAGCAAAGAAATGATA+TGG	+	tig0032878:3757-3776	Msa1450310:three_prime_UTR	25.0%
!!!	TGTTTTGAATCTGAAGAATT+CGG	+	tig0032878:3911-3930	Msa1450310:three_prime_UTR	25.0%
!	TACAAGATCAAAATACCAAA+GGG	-	tig0032878:3967-3986	None:intergenic	25.0%
!	TTACAAGATCAAAATACCAA+AGG	-	tig0032878:3968-3987	None:intergenic	25.0%
	TTACAATACCAAGAATCACT+GGG	-	tig0032878:1304-1323	None:intergenic	30.0%
	TTTACAATACCAAGAATCAC+TGG	-	tig0032878:1305-1324	None:intergenic	30.0%
	TATGCTTTGTGACATATTTC+TGG	+	tig0032878:1577-1596	Msa1450310:intron	30.0%
	AAATAGAAGGCAAAGTTGAA+GGG	-	tig0032878:1629-1648	None:intergenic	30.0%
	AAAATAGAAGGCAAAGTTGA+AGG	-	tig0032878:1630-1649	None:intergenic	30.0%
	TCACAAATAATTACTGTTCG+AGG	-	tig0032878:1741-1760	None:intergenic	30.0%
!	GCTTTTATTGATATCACCAT+TGG	+	tig0032878:1812-1831	Msa1450310:intron	30.0%
	TCATTCTTCTCTAGTGAATT+TGG	+	tig0032878:2048-2067	Msa1450310:CDS	30.0%
!	ATAAAAAAGAAGCATGACAC+TGG	+	tig0032878:2115-2134	Msa1450310:CDS	30.0%
	AATGTCATTGAAGAGAAAGT+TGG	+	tig0032878:2187-2206	Msa1450310:CDS	30.0%
!	AAGAGAAAAGTGTAGAAACT+GGG	+	tig0032878:2464-2483	Msa1450310:CDS	30.0%
!	GTGTTGATCCTGAAATTAAA+TGG	+	tig0032878:2512-2531	Msa1450310:CDS	30.0%
	TTGATCCTGAAATTAAATGG+AGG	+	tig0032878:2515-2534	Msa1450310:CDS	30.0%
	TGATCCTGAAATTAAATGGA+GGG	+	tig0032878:2516-2535	Msa1450310:CDS	30.0%
	AGCAATTGTCTGATGAATAT+AGG	+	tig0032878:2575-2594	Msa1450310:CDS	30.0%
	AAATATACTCACATGGAAGA+TGG	-	tig0032878:2623-2642	None:intergenic	30.0%
!	GATTTAAAAGCACAGTAGTA+GGG	-	tig0032878:3214-3233	None:intergenic	30.0%
!	AGATTTAAAAGCACAGTAGT+AGG	-	tig0032878:3215-3234	None:intergenic	30.0%
!!	TTATACAATGGCCTTTGTTT+TGG	+	tig0032878:3629-3648	Msa1450310:three_prime_UTR	30.0%
!!!	TATACAATGGCCTTTGTTTT+GGG	+	tig0032878:3630-3649	Msa1450310:three_prime_UTR	30.0%
	TCAAAACAAACCCAAAACAA+AGG	-	tig0032878:3643-3662	None:intergenic	30.0%
!!!	TTGTTTTGGGTTTGTTTTGA+TGG	+	tig0032878:3643-3662	Msa1450310:three_prime_UTR	30.0%
!!!	TGTTTTGGGTTTGTTTTGAT+GGG	+	tig0032878:3644-3663	Msa1450310:three_prime_UTR	30.0%
!	TGTTTTTCTGTACACCAAAA+AGG	-	tig0032878:3709-3728	None:intergenic	30.0%
	AAATATTGCATCATCAGCAA+AGG	-	tig0032878:3738-3757	None:intergenic	30.0%
	TTGCCAGACTAATTTCCAAA+TGG	+	tig0032878:1327-1346	Msa1450310:exon	35.0%
	TCTCCATTTGGAAATTAGTC+TGG	-	tig0032878:1333-1352	None:intergenic	35.0%
!	AATAGAGCATTACCCACATA+AGG	-	tig0032878:1482-1501	None:intergenic	35.0%
	CAGTGCTTCAAATAAACCAA+TGG	-	tig0032878:1831-1850	None:intergenic	35.0%
	TGTGAGAAGGAATATCATGT+TGG	+	tig0032878:1869-1888	Msa1450310:CDS	35.0%
	GTGAGAAGGAATATCATGTT+GGG	+	tig0032878:1870-1889	Msa1450310:CDS	35.0%
	ATATCATGTTGGGTGTTTGA+AGG	+	tig0032878:1880-1899	Msa1450310:CDS	35.0%
	GCACAACATGCAGAATTTAA+AGG	+	tig0032878:1904-1923	Msa1450310:CDS	35.0%
!!!	TTGTTTGCTTTTTGTTGACC+TGG	+	tig0032878:1965-1984	Msa1450310:intron	35.0%
!!!	TGTTTGCTTTTTGTTGACCT+GGG	+	tig0032878:1966-1985	Msa1450310:intron	35.0%
	TAGTGAATTTGGTTGCTTCT+GGG	+	tig0032878:2059-2078	Msa1450310:CDS	35.0%
!	GAAGAGAAAAGTGTAGAAAC+TGG	+	tig0032878:2463-2482	Msa1450310:CDS	35.0%
!	AGAGAAAAGTGTAGAAACTG+GGG	+	tig0032878:2465-2484	Msa1450310:CDS	35.0%
!	AGAACCCTCCATTTAATTTC+AGG	-	tig0032878:2523-2542	None:intergenic	35.0%
!!	CAATTGCTTATTTTCACCTG+AGG	-	tig0032878:2563-2582	None:intergenic	35.0%
!	TTTATGTTTCTACCCCGTAT+CGG	+	tig0032878:2640-2659	Msa1450310:intron	35.0%
	AACAAACAGAAAACCGATAC+GGG	-	tig0032878:2656-2675	None:intergenic	35.0%
	GAACAAACAGAAAACCGATA+CGG	-	tig0032878:2657-2676	None:intergenic	35.0%
!!	CTACTGTGTTGATAATACTC+TGG	+	tig0032878:2679-2698	Msa1450310:intron	35.0%
	TTACACAGAAAAAACATCCG+CGG	+	tig0032878:2936-2955	Msa1450310:intron	35.0%
	AAAGAAGCAAGGTATGCATT+TGG	-	tig0032878:3032-3051	None:intergenic	35.0%
	ATCAGGTTATGAAAGAAGCA+AGG	-	tig0032878:3043-3062	None:intergenic	35.0%
!!	CTGGAGTATTTCGTGTTTTT+GGG	+	tig0032878:3106-3125	Msa1450310:CDS	35.0%
!!	CCATATTGATTCAGCTTCAT+CGG	-	tig0032878:3370-3389	None:intergenic	35.0%
!!	TGATAAGTACTTGGTAGTGT+CGG	-	tig0032878:3579-3598	None:intergenic	35.0%
	ACAATCTCCTGATAAGTACT+TGG	-	tig0032878:3588-3607	None:intergenic	35.0%
	ACAAAGGCCATTGTATAAGA+GGG	-	tig0032878:3627-3646	None:intergenic	35.0%
	AACAAAGGCCATTGTATAAG+AGG	-	tig0032878:3628-3647	None:intergenic	35.0%
!	AAGAATTCGGCTTTTGTTCT+TGG	+	tig0032878:3924-3943	Msa1450310:three_prime_UTR	35.0%
!	AGAATTCGGCTTTTGTTCTT+GGG	+	tig0032878:3925-3944	Msa1450310:three_prime_UTR	35.0%
!!	ACATAATTTGTTGCACCCTT+TGG	+	tig0032878:3949-3968	Msa1450310:three_prime_UTR	35.0%
	GTTGTGCATTACTCTCCATT+TGG	-	tig0032878:1345-1364	None:intergenic	40.0%
!!	CGTCGGTTTATCTGTTCTAA+AGG	-	tig0032878:1412-1431	None:intergenic	40.0%
	TGACAATACGAATGCATCGT+CGG	-	tig0032878:1429-1448	None:intergenic	40.0%
	AAGGCAAAGTTGAAGGGAAA+TGG	-	tig0032878:1623-1642	None:intergenic	40.0%
	AGTTGACAAAAAAGGGTTGG+CGG	-	tig0032878:1792-1811	None:intergenic	40.0%
	AGCAGTTGACAAAAAAGGGT+TGG	-	tig0032878:1795-1814	None:intergenic	40.0%
!!!	GTTTGCTTTTTGTTGACCTG+GGG	+	tig0032878:1967-1986	Msa1450310:intron	40.0%
	CTAGTGAATTTGGTTGCTTC+TGG	+	tig0032878:2058-2077	Msa1450310:CDS	40.0%
	AGTGAATTTGGTTGCTTCTG+GGG	+	tig0032878:2060-2079	Msa1450310:CDS	40.0%
	AGAGACACTGTTGAAGAGAA+AGG	+	tig0032878:2262-2281	Msa1450310:CDS	40.0%
	TGTCTACAGCTTTCTCTTCA+AGG	-	tig0032878:2327-2346	None:intergenic	40.0%
	GTTGAGAAAGATGTAGACAC+TGG	+	tig0032878:2436-2455	Msa1450310:CDS	40.0%
!!	TTAAATGGAGGGTTCTGAAC+TGG	+	tig0032878:2527-2546	Msa1450310:CDS	40.0%
	ATATAGGCAAGTGCTTTCGA+AGG	+	tig0032878:2591-2610	Msa1450310:CDS	40.0%
	ACAAACAGAAAACCGATACG+GGG	-	tig0032878:2655-2674	None:intergenic	40.0%
!	ACTTGCTAACCTTTAAGAGC+TGG	+	tig0032878:2743-2762	Msa1450310:intron	40.0%
	CAAAACACTCCAGCTCTTAA+AGG	-	tig0032878:2755-2774	None:intergenic	40.0%
	CCAGAAGACGAATCAACTAT+AGG	-	tig0032878:2797-2816	None:intergenic	40.0%
!!	CCTATAGTTGATTCGTCTTC+TGG	+	tig0032878:2794-2813	Msa1450310:CDS	40.0%
	ACATTGTCGGGATGAAATCA+CGG	-	tig0032878:2820-2839	None:intergenic	40.0%
	TTCATCCCGACAATGTTGTT+TGG	+	tig0032878:2824-2843	Msa1450310:CDS	40.0%
!	TCATCCCGACAATGTTGTTT+GGG	+	tig0032878:2825-2844	Msa1450310:CDS	40.0%
	CTTACCCAAACAACATTGTC+GGG	-	tig0032878:2832-2851	None:intergenic	40.0%
	GCTTACCCAAACAACATTGT+CGG	-	tig0032878:2833-2852	None:intergenic	40.0%
	TACACAGAAAAAACATCCGC+GGG	+	tig0032878:2937-2956	Msa1450310:intron	40.0%
	TCACTGTCAAGTAAGTGATG+AGG	+	tig0032878:2991-3010	Msa1450310:intron	40.0%
	CACTGTCAAGTAAGTGATGA+GGG	+	tig0032878:2992-3011	Msa1450310:intron	40.0%
!!	GCTGGAGTATTTCGTGTTTT+TGG	+	tig0032878:3105-3124	Msa1450310:CDS	40.0%
	CCGATGAAGCTGAATCAATA+TGG	+	tig0032878:3367-3386	Msa1450310:CDS	40.0%
	GACACTACCAAGTACTTATC+AGG	+	tig0032878:3578-3597	Msa1450310:CDS	40.0%
	TTAGAGGCCCTCTTATACAA+TGG	+	tig0032878:3617-3636	Msa1450310:three_prime_UTR	40.0%
!!	TACAGAGTGTTCTGCCTTTT+TGG	+	tig0032878:3692-3711	Msa1450310:three_prime_UTR	40.0%
	TATTGCATCATCAGCAAAGG+TGG	-	tig0032878:3735-3754	None:intergenic	40.0%
	GAGGAAACTACTAACTGACT+AGG	+	tig0032878:3798-3817	Msa1450310:three_prime_UTR	40.0%
	ACTACTAACTGACTAGGACA+TGG	+	tig0032878:3804-3823	Msa1450310:three_prime_UTR	40.0%
	CTACTAACTGACTAGGACAT+GGG	+	tig0032878:3805-3824	Msa1450310:three_prime_UTR	40.0%
	CAAGAATCACTGGGAATGCA+TGG	-	tig0032878:1295-1314	None:intergenic	45.0%
	CATGCATTCCCAGTGATTCT+TGG	+	tig0032878:1293-1312	Msa1450310:five_prime_UTR	45.0%
	ACAACGTAATGCGAATGCTC+TGG	+	tig0032878:1360-1379	Msa1450310:CDS	45.0%
!	AAAAGTGTAGCTGTCGACCA+TGG	+	tig0032878:1448-1467	Msa1450310:CDS	45.0%
	AAAGTGTAGCTGTCGACCAT+GGG	+	tig0032878:1449-1468	Msa1450310:CDS	45.0%
	GGCGGTCTATAATTCACGTA+AGG	-	tig0032878:1774-1793	None:intergenic	45.0%
!!	TTGACCTGGGGTTATTTTCC+AGG	+	tig0032878:1979-1998	Msa1450310:intron	45.0%
	AACTCCTGGAAAATAACCCC+AGG	-	tig0032878:1986-2005	None:intergenic	45.0%
!	ATTTTCCAGGAGTTGCCTGA+AGG	+	tig0032878:1992-2011	Msa1450310:intron	45.0%
!	TTTTCCAGGAGTTGCCTGAA+GGG	+	tig0032878:1993-2012	Msa1450310:intron	45.0%
	AAGCATGACACTGGCGTTAA+AGG	+	tig0032878:2124-2143	Msa1450310:CDS	45.0%
	AGCATGACACTGGCGTTAAA+GGG	+	tig0032878:2125-2144	Msa1450310:CDS	45.0%
	GAAGAGAAAGTTGGAGACAC+CGG	+	tig0032878:2196-2215	Msa1450310:CDS	45.0%
	GACACTGTTGAAGAGAAAGG+TGG	+	tig0032878:2265-2284	Msa1450310:CDS	45.0%
	GAAGAGAAAGTTGCAGACAC+TGG	+	tig0032878:2301-2320	Msa1450310:CDS	45.0%
	GAAGAGAAAGCTGTAGACAC+CGG	+	tig0032878:2328-2347	Msa1450310:CDS	45.0%
	TACAGCTTTCTCTTCAACGC+CGG	-	tig0032878:2350-2369	None:intergenic	45.0%
	GAAGAGAAAGCTGTAGACAC+CGG	+	tig0032878:2328-2347	Msa1450310:CDS	45.0%
!	ACTGGCGTTGAAGAGAAATG+TGG	+	tig0032878:2400-2419	Msa1450310:CDS	45.0%
	GAAGAGAAATGTGGAGACAC+TGG	+	tig0032878:2409-2428	Msa1450310:CDS	45.0%
	GAAACTGGGGTTGAAGAGAA+AGG	+	tig0032878:2478-2497	Msa1450310:CDS	45.0%
!	GAAGAGAAAGGTGTAGTCAC+TGG	+	tig0032878:2490-2509	Msa1450310:CDS	45.0%
	AACTGGAAGTTAGTTGCCTC+AGG	+	tig0032878:2544-2563	Msa1450310:CDS	45.0%
!!	AGCTGGAGTGTTTTGTCTAC+AGG	+	tig0032878:2760-2779	Msa1450310:intron	45.0%
!!	AGCCAAGTTGTTGTTTCTGC+TGG	+	tig0032878:3087-3106	Msa1450310:intron	45.0%
	CTCCAGCAGAAACAACAACT+TGG	-	tig0032878:3092-3111	None:intergenic	45.0%
	AAAGGAAGTTCTGCGACTTC+TGG	-	tig0032878:3132-3151	None:intergenic	45.0%
	AGAAGTCGCAGAACTTCCTT+TGG	+	tig0032878:3131-3150	Msa1450310:CDS	45.0%
	AGTTTCTGAGTACCAAGGAC+AGG	+	tig0032878:3161-3180	Msa1450310:CDS	45.0%
	CGTGAAAAACCTTGTCCTAC+CGG	+	tig0032878:3341-3360	Msa1450310:CDS	45.0%
	ACTTATAAGCTGCCATCGAG+CGG	+	tig0032878:3874-3893	Msa1450310:three_prime_UTR	45.0%
	ATCGCATTAAAACCGCTCGA+TGG	-	tig0032878:3889-3908	None:intergenic	45.0%
	AAGTGTAGCTGTCGACCATG+GGG	+	tig0032878:1450-1469	Msa1450310:CDS	50.0%
	ATGGGGGATGTGCCTTATGT+GGG	+	tig0032878:1467-1486	Msa1450310:CDS	50.0%
	CGTGTTCTTGCAGTGTGAGA+AGG	+	tig0032878:1856-1875	Msa1450310:intron	50.0%
	TTTCCAGGAGTTGCCTGAAG+GGG	+	tig0032878:1994-2013	Msa1450310:intron	50.0%
	GACACTGGCGTTAAAGGGAA+AGG	+	tig0032878:2130-2149	Msa1450310:CDS	50.0%
!	AAAGGGAAAGGTGCAGACAC+TGG	+	tig0032878:2142-2161	Msa1450310:CDS	50.0%
	GAAGAGAAAGGTGGAGACAC+TGG	+	tig0032878:2223-2242	Msa1450310:CDS	50.0%
	GAAGAGAAAGGTGGAGACAC+AGG	+	tig0032878:2223-2242	Msa1450310:CDS	50.0%
	TGCAGCTTTCTCTTCAACGC+CGG	-	tig0032878:2377-2396	None:intergenic	50.0%
	GAAGAGAAAGCTGCAGACAC+TGG	+	tig0032878:2382-2401	Msa1450310:CDS	50.0%
	GAGAAATGTGGAGACACTGG+TGG	+	tig0032878:2412-2431	Msa1450310:CDS	50.0%
	TTCCAGCAAAATCTTGCCCG+CGG	-	tig0032878:2956-2975	None:intergenic	50.0%
	AGTCGCAGAACTTCCTTTGG+TGG	+	tig0032878:3134-3153	Msa1450310:CDS	50.0%
	TCAGAAACTGTCGCCACCAA+AGG	-	tig0032878:3150-3169	None:intergenic	50.0%
	GCGACAGTTTCTGAGTACCA+AGG	+	tig0032878:3156-3175	Msa1450310:CDS	50.0%
	TACAAAGCTCGAGCACGATG+AGG	+	tig0032878:3407-3426	Msa1450310:CDS	50.0%
	AAGGGACCTCAGTACTACAG+AGG	+	tig0032878:3547-3566	Msa1450310:CDS	50.0%
	AACAGGCCTCTGTAGTACTG+AGG	-	tig0032878:3556-3575	None:intergenic	50.0%
!!	GTACTTGGTAGTGTCGGAAC+AGG	-	tig0032878:3573-3592	None:intergenic	50.0%
	ACTGACTAGGACATGGGAGA+AGG	+	tig0032878:3811-3830	Msa1450310:three_prime_UTR	50.0%
	CTGACTAGGACATGGGAGAA+GGG	+	tig0032878:3812-3831	Msa1450310:three_prime_UTR	50.0%
	TCACTGGGAATGCATGGCAG+AGG	-	tig0032878:1289-1308	None:intergenic	55.0%
	AATGCGAATGCTCTGGCAGC+TGG	+	tig0032878:1367-1386	Msa1450310:CDS	55.0%
	CTGGCAGCTGGAAGGATTGA+AGG	+	tig0032878:1379-1398	Msa1450310:CDS	55.0%
	AGTGTAGCTGTCGACCATGG+GGG	+	tig0032878:1451-1470	Msa1450310:CDS	55.0%
	CACATAAGGCACATCCCCCA+TGG	-	tig0032878:1468-1487	None:intergenic	55.0%
	CATGGGGGATGTGCCTTATG+TGG	+	tig0032878:1466-1485	Msa1450310:CDS	55.0%
	AATCCCCTTCAGGCAACTCC+TGG	-	tig0032878:2000-2019	None:intergenic	55.0%
	AGGAGTTGCCTGAAGGGGAT+TGG	+	tig0032878:1999-2018	Msa1450310:intron	55.0%
	CTGCAGAACCAATCCCCTTC+AGG	-	tig0032878:2010-2029	None:intergenic	55.0%
!	GTGCAGACACTGGCGTTGAA+GGG	+	tig0032878:2152-2171	Msa1450310:CDS	55.0%
!	GACACTGGCGTTGAAGGGAA+AGG	+	tig0032878:2157-2176	Msa1450310:CDS	55.0%
	TCCACCTTTCTCTTCGACGC+CGG	-	tig0032878:2218-2237	None:intergenic	55.0%
!	GACACTGGCGTCGAAGAGAA+AGG	+	tig0032878:2238-2257	Msa1450310:CDS	55.0%
!	ATCCGCGGGCAAGATTTTGC+TGG	+	tig0032878:2951-2970	Msa1450310:CDS	55.0%
	GATTCAGCTTCATCGGCAGC+CGG	-	tig0032878:3363-3382	None:intergenic	55.0%
	CGAATGCTCTGGCAGCTGGA+AGG	+	tig0032878:1371-1390	Msa1450310:CDS	60.0%
!	GGTGCAGACACTGGCGTTGA+AGG	+	tig0032878:2151-2170	Msa1450310:CDS	60.0%
!	GGCGTTGAAGGGAAAGGTGC+AGG	+	tig0032878:2163-2182	Msa1450310:CDS	60.0%
!	GACACCGGCGTCGAAGAGAA+AGG	+	tig0032878:2211-2230	Msa1450310:CDS	60.0%
!	ACCGGCGTCGAAGAGAAAGG+TGG	+	tig0032878:2214-2233	Msa1450310:CDS	60.0%
	CATCGGCAGCCGGTAGGACA+AGG	-	tig0032878:3353-3372	None:intergenic	65.0%
	CAGCTTCATCGGCAGCCGGT+AGG	-	tig0032878:3359-3378	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
tig0032878	gene	1278	4022	1278	ID=Msa1450310;Name=Msa1450310
tig0032878	mRNA	1278	4022	1278	ID=Msa1450310-mRNA-1;Parent=Msa1450310;Name=Msa1450310-mRNA-1;_AED=0.19;_eAED=0.19;_QI=68\|0.62\|0.55\|0.66\|1\|1\|9\|411\|448
tig0032878	exon	1278	1488	1278	ID=Msa1450310-mRNA-1:exon:17414;Parent=Msa1450310-mRNA-1
tig0032878	exon	1710	1764	1710	ID=Msa1450310-mRNA-1:exon:17415;Parent=Msa1450310-mRNA-1
tig0032878	exon	1869	1925	1869	ID=Msa1450310-mRNA-1:exon:17416;Parent=Msa1450310-mRNA-1
tig0032878	exon	2001	2630	2001	ID=Msa1450310-mRNA-1:exon:17417;Parent=Msa1450310-mRNA-1
tig0032878	exon	2782	2846	2782	ID=Msa1450310-mRNA-1:exon:17418;Parent=Msa1450310-mRNA-1
tig0032878	exon	2944	3000	2944	ID=Msa1450310-mRNA-1:exon:17419;Parent=Msa1450310-mRNA-1
tig0032878	exon	3089	3182	3089	ID=Msa1450310-mRNA-1:exon:17420;Parent=Msa1450310-mRNA-1
tig0032878	exon	3288	3428	3288	ID=Msa1450310-mRNA-1:exon:17421;Parent=Msa1450310-mRNA-1
tig0032878	exon	3507	4022	3507	ID=Msa1450310-mRNA-1:exon:17422;Parent=Msa1450310-mRNA-1
tig0032878	five_prime_UTR	1278	1345	1278	ID=Msa1450310-mRNA-1:five_prime_utr;Parent=Msa1450310-mRNA-1
tig0032878	CDS	1346	1488	1346	ID=Msa1450310-mRNA-1:cds;Parent=Msa1450310-mRNA-1
tig0032878	CDS	1710	1764	1710	ID=Msa1450310-mRNA-1:cds;Parent=Msa1450310-mRNA-1
tig0032878	CDS	1869	1925	1869	ID=Msa1450310-mRNA-1:cds;Parent=Msa1450310-mRNA-1
tig0032878	CDS	2001	2630	2001	ID=Msa1450310-mRNA-1:cds;Parent=Msa1450310-mRNA-1
tig0032878	CDS	2782	2846	2782	ID=Msa1450310-mRNA-1:cds;Parent=Msa1450310-mRNA-1
tig0032878	CDS	2944	3000	2944	ID=Msa1450310-mRNA-1:cds;Parent=Msa1450310-mRNA-1
tig0032878	CDS	3089	3182	3089	ID=Msa1450310-mRNA-1:cds;Parent=Msa1450310-mRNA-1
tig0032878	CDS	3288	3428	3288	ID=Msa1450310-mRNA-1:cds;Parent=Msa1450310-mRNA-1
tig0032878	CDS	3507	3611	3507	ID=Msa1450310-mRNA-1:cds;Parent=Msa1450310-mRNA-1
tig0032878	three_prime_UTR	3612	4022	3612	ID=Msa1450310-mRNA-1:three_prime_utr;Parent=Msa1450310-mRNA-1

Gene Sequence

>Msa1450310

ATGGAGAGTAATGCACAACGTAATGCGAATGCTCTGGCAGCTGGAAGGATTGAAGGCATTGATCCTTTAGAACAGATAAACCGACGATGCATTCGTATTGTCAAAAGTGTAGCTGTCGACCATGGGGGATGTGCCTTATGTGGATGTCATGATTTCGTTAAAATATTTGGTCCTCGAACAGTAATTATTTGTGATCAGTGTGAGAAGGAATATCATGTTGGGTGTTTGAAGGAGCACAACATGCAGAATTTAAAGGAGTTGCCTGAAGGGGATTGGTTCTGCAGTACAAGTTGCAGTCATATTCATTCTTCTCTAGTGAATTTGGTTGCTTCTGGGGAGAATAGTCTCCCAGATTCTATTCTAAGTTTGATAAAAAAGAAGCATGACACTGGCGTTAAAGGGAAAGGTGCAGACACTGGCGTTGAAGGGAAAGGTGCAGGCAATGTCATTGAAGAGAAAGTTGGAGACACCGGCGTCGAAGAGAAAGGTGGAGACACTGGCGTCGAAGAGAAAGGTAGAGACACTGTTGAAGAGAAAGGTGGAGACACAGGTGTTGAAGAGAAAGTTGCAGACACTGGCCTTGAAGAGAAAGCTGTAGACACCGGCGTTGAAGAGAAAGCTGTAGACACCGGCGTTGAAGAGAAAGCTGCAGACACTGGCGTTGAAGAGAAATGTGGAGACACTGGTGGTGTTGAGAAAGATGTAGACACTGGTGTTGAAGAGAAAAGTGTAGAAACTGGGGTTGAAGAGAAAGGTGTAGTCACTGGTGTTGATCCTGAAATTAAATGGAGGGTTCTGAACTGGAAGTTAGTTGCCTCAGGTGAAAATAAGCAATTGTCTGATGAATATAGGCAAGTGCTTTCGAAGGCTGTTGCCATCTTCCATGAGAGATTTGATCCTATAGTTGATTCGTCTTCTGGCCGTGATTTCATCCCGACAATGTTGTTTGGAAAAAACATCCGCGGGCAAGATTTTGCTGGAATGTACTGTGCAGTGCTCACTGTCAACCAAGTTGTTGTTTCTGCTGGAGTATTTCGTGTTTTTGGGCCAGAAGTCGCAGAACTTCCTTTGGTGGCGACAGTTTCTGAGTACCAAGGACAGGGTTACTTTCAATGCTTGTTTTCTTGCATTGAGAGACTACTTCAATCTCTAAGCGTGAAAAACCTTGTCCTACCGGCTGCCGATGAAGCTGAATCAATATGGACTAACAAATTTGGGTTTACAAAGCTCGAGCACGATGAGATCAACAATTACAAGAAGTTTTATCATATGATGATCTTTCAAGGGACCTCAGTACTACAGAGGCCTGTTCCGACACTACCAAGTACTTATCAGGAGATTGTGTAA

Protein sequence

>Msa1450310

MESNAQRNANALAAGRIEGIDPLEQINRRCIRIVKSVAVDHGGCALCGCHDFVKIFGPRTVIICDQCEKEYHVGCLKEHNMQNLKELPEGDWFCSTSCSHIHSSLVNLVASGENSLPDSILSLIKKKHDTGVKGKGADTGVEGKGAGNVIEEKVGDTGVEEKGGDTGVEEKGRDTVEEKGGDTGVEEKVADTGLEEKAVDTGVEEKAVDTGVEEKAADTGVEEKCGDTGGVEKDVDTGVEEKSVETGVEEKGVVTGVDPEIKWRVLNWKLVASGENKQLSDEYRQVLSKAVAIFHERFDPIVDSSSGRDFIPTMLFGKNIRGQDFAGMYCAVLTVNQVVVSAGVFRVFGPEVAELPLVATVSEYQGQGYFQCLFSCIERLLQSLSVKNLVLPAADEAESIWTNKFGFTKLEHDEINNYKKFYHMMIFQGTSVLQRPVPTLPSTYQEIV*