AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene003593

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003593.t1 MTR_4g072680 96.053 76 3 0 1 76 1 76 1.88e-49 153
MS.gene003593.t1 MTR_4g072480 96.053 76 3 0 1 76 1 76 1.98e-49 153
MS.gene003593.t1 MTR_4g072460 93.421 76 5 0 1 76 1 76 3.95e-48 150
MS.gene003593.t1 MTR_4g072620 90.789 76 7 0 1 76 1 76 2.00e-47 148
MS.gene003593.t1 MTR_4g072730 86.842 76 10 0 1 76 1 76 4.10e-45 142
MS.gene003593.t1 MTR_4g072570 80.263 76 15 0 1 76 1 76 1.24e-39 128
MS.gene003593.t1 MTR_4g072660 81.579 76 9 1 1 76 1 71 2.72e-39 127
MS.gene003593.t1 MTR_4g072530 78.947 76 16 0 1 76 1 76 4.95e-38 124
MS.gene003593.t1 MTR_4g072500 78.947 76 16 0 1 76 1 76 5.61e-38 124
MS.gene003593.t1 MTR_3g084150 73.684 76 20 0 1 76 1 76 1.53e-36 120
MS.gene003593.t1 MTR_4g072640 73.684 76 20 0 1 76 1 76 7.70e-36 119
MS.gene003593.t1 MTR_8g461410 72.368 76 21 0 1 76 1 76 7.78e-36 119
MS.gene003593.t1 MTR_3g084180 72.368 76 21 0 1 76 1 76 8.31e-36 119
MS.gene003593.t1 MTR_3g110042 73.684 76 20 0 1 76 1 76 3.26e-35 117
MS.gene003593.t1 MTR_4g072310 70.130 77 22 1 1 76 1 77 1.81e-34 115
MS.gene003593.t1 MTR_4g072240 68.831 77 23 1 1 76 1 77 2.61e-34 115
MS.gene003593.t1 MTR_4g072870 68.831 77 23 1 1 76 1 77 3.25e-34 114
MS.gene003593.t1 MTR_4g072230 71.429 77 19 2 1 76 1 75 4.41e-34 114
MS.gene003593.t1 MTR_4g072880 70.130 77 22 1 1 76 1 77 1.74e-33 113
MS.gene003593.t1 MTR_3g084250 67.949 78 25 0 1 78 1 78 2.34e-33 112
MS.gene003593.t1 MTR_4g072220 66.234 77 25 1 1 76 1 77 4.64e-33 112
MS.gene003593.t1 MTR_4g072300 66.234 77 25 1 1 76 1 77 4.99e-33 112
MS.gene003593.t1 MTR_3g084240 67.105 76 25 0 1 76 1 76 6.12e-33 111
MS.gene003593.t1 MTR_4g072370 65.385 78 25 1 1 78 1 76 6.81e-33 111
MS.gene003593.t1 MTR_3g117630 73.239 71 19 0 6 76 6 76 6.98e-33 111
MS.gene003593.t1 MTR_4g072270 68.831 77 23 1 1 76 1 77 1.12e-32 110
MS.gene003593.t1 MTR_4g072490 66.667 78 21 1 1 78 1 73 1.96e-32 110
MS.gene003593.t1 MTR_4g072630 66.667 78 22 1 1 78 1 74 2.30e-32 110
MS.gene003593.t1 MTR_4g072580 67.105 76 20 1 1 76 1 71 2.56e-32 110
MS.gene003593.t1 MTR_3g084170 67.105 76 25 0 1 76 1 76 2.95e-32 110
MS.gene003593.t1 MTR_4g072550 89.474 57 6 0 20 76 31 87 3.76e-32 110
MS.gene003593.t1 MTR_4g072260 64.935 77 26 1 1 76 1 77 3.78e-32 109
MS.gene003593.t1 MTR_4g072890 64.935 77 26 1 1 76 1 77 4.44e-32 109
MS.gene003593.t1 MTR_5g008360 80.952 63 12 0 13 75 4 66 5.41e-32 108
MS.gene003593.t1 MTR_4g072860 68.831 77 23 1 1 76 1 77 6.67e-32 108
MS.gene003593.t1 MTR_4g072250 71.429 77 20 2 1 76 1 76 9.00e-32 108
MS.gene003593.t1 MTR_3g084220 67.105 76 25 0 1 76 1 76 1.11e-31 108
MS.gene003593.t1 MTR_4g072740 66.667 78 21 1 1 78 1 73 1.67e-31 107
MS.gene003593.t1 MTR_3g084210 66.250 80 27 0 1 80 1 80 2.54e-31 107
MS.gene003593.t1 MTR_8g461420 67.500 80 26 0 1 80 1 80 2.60e-31 107
MS.gene003593.t1 MTR_3g110038 64.474 76 27 0 1 76 1 76 3.13e-31 107
MS.gene003593.t1 MTR_4g072330 61.538 78 28 1 1 78 1 76 5.26e-31 106
MS.gene003593.t1 MTR_4g072830 62.500 80 28 1 1 80 1 78 5.56e-31 106
MS.gene003593.t1 MTR_4g072600 63.750 80 24 1 1 80 1 75 5.57e-31 106
MS.gene003593.t1 MTR_3g084190 67.500 80 26 0 1 80 1 80 6.29e-31 106
MS.gene003593.t1 MTR_3g084230 67.500 80 26 0 1 80 1 80 6.57e-31 106
MS.gene003593.t1 MTR_4g072610 62.963 81 24 1 1 81 1 75 7.85e-31 106
MS.gene003593.t1 MTR_3g110032 63.291 79 28 1 1 78 1 79 9.07e-31 106
MS.gene003593.t1 MTR_4g072465 62.821 78 25 1 1 78 1 74 2.39e-30 105
MS.gene003593.t1 MTR_3g084160 67.500 80 26 0 1 80 1 80 2.52e-30 105
MS.gene003593.t1 MTR_3g084260 64.935 77 26 1 1 76 1 77 4.93e-30 104
MS.gene003593.t1 MTR_4g072470 65.789 76 21 1 1 76 1 71 4.99e-30 103
MS.gene003593.t1 MTR_4g050700 64.935 77 26 1 1 76 1 77 3.48e-29 102
MS.gene003593.t1 MTR_2g012410 64.935 77 26 1 1 76 1 77 3.48e-29 102
MS.gene003593.t1 MTR_4g072750 63.415 82 28 1 1 80 1 82 3.56e-29 102
MS.gene003593.t1 MTR_4g072540 65.000 80 24 1 1 80 1 76 3.59e-29 102
MS.gene003593.t1 MTR_4g072820 67.532 77 18 2 1 76 1 71 6.61e-29 101
MS.gene003593.t1 MTR_5g008350 57.317 82 30 1 1 82 1 77 7.62e-29 100
MS.gene003593.t1 MTR_4g072720 65.000 80 24 1 1 80 1 76 1.31e-28 100
MS.gene003593.t1 MTR_4g072850 66.667 78 24 1 1 76 1 78 1.88e-28 100
MS.gene003593.t1 MTR_3g110045 66.667 78 26 0 3 80 62 139 2.43e-28 102
MS.gene003593.t1 MTR_3g084200 61.111 72 28 0 1 72 1 72 6.03e-28 99.4
MS.gene003593.t1 MTR_4g072650 61.538 78 24 1 1 78 1 72 1.57e-27 97.8
MS.gene003593.t1 MTR_4g072590 63.750 80 24 1 1 80 1 75 2.65e-27 97.1
MS.gene003593.t1 MTR_4g072670 61.250 80 27 1 1 80 1 76 3.51e-27 97.1
MS.gene003593.t1 MTR_4g072510 62.500 80 26 1 1 80 1 76 8.87e-27 95.9
MS.gene003593.t1 MTR_4g072380 63.636 77 22 2 1 76 1 72 1.07e-26 95.9
MS.gene003593.t1 MTR_5g008370 75.000 60 15 0 18 77 18 77 1.53e-26 97.1
MS.gene003593.t1 MTR_4g072940 77.193 57 13 0 21 77 53 109 1.75e-26 96.3
MS.gene003593.t1 MTR_4g072770 60.526 76 20 1 1 76 1 66 1.04e-24 90.5
MS.gene003593.t1 MTR_4g072980 55.696 79 29 2 1 78 8 81 7.20e-24 88.6
MS.gene003593.t1 MTR_4g072690 61.250 80 26 1 1 80 1 75 8.36e-24 88.2
MS.gene003593.t1 MTR_4g072280 54.667 75 33 1 1 74 1 75 9.27e-24 88.2
MS.gene003593.t1 MTR_3g117640 74.545 55 13 1 25 78 34 88 6.11e-23 86.7
MS.gene003593.t1 MTR_4g072450 58.750 80 28 1 1 80 1 75 1.17e-22 85.5
MS.gene003593.t1 MTR_4g072560 52.632 76 35 1 6 80 13 88 1.22e-22 85.9
MS.gene003593.t1 MTR_4g072910 54.054 74 33 1 9 81 18 91 1.97e-22 85.1
MS.gene003593.t1 MTR_4g072290 51.948 77 22 2 1 76 1 63 7.87e-20 78.2
MS.gene003593.t1 MTR_6g077850 50.877 57 28 0 24 80 33 89 1.04e-18 75.9
MS.gene003593.t1 MTR_3g117610 60.714 56 21 1 23 78 43 97 1.08e-18 75.9
MS.gene003593.t1 MTR_3g113310 59.259 54 21 1 23 76 40 92 2.93e-18 74.7
MS.gene003593.t1 MTR_3g110035 60.000 55 22 0 26 80 72 126 3.49e-18 75.5
MS.gene003593.t1 MTR_4g072190 58.929 56 22 1 23 78 34 88 1.08e-17 73.2
MS.gene003593.t1 MTR_4g072930 54.237 59 23 1 27 81 43 101 2.11e-16 70.1
MS.gene003593.t1 MTR_8g076040 58.491 53 21 1 24 76 40 91 1.72e-15 67.8
MS.gene003593.t1 MTR_5g021820 50.000 58 28 1 24 81 39 95 2.11e-15 67.4
MS.gene003593.t1 MTR_2g043960 54.545 55 24 1 24 78 75 128 4.71e-13 62.8
MS.gene003593.t1 MTR_4g124750 44.156 77 40 2 4 78 46 121 4.95e-13 62.4
MS.gene003593.t1 MTR_1g024085 36.283 113 58 3 24 135 36 135 1.95e-12 60.8
MS.gene003593.t1 MTR_1g009150 53.571 56 25 1 25 80 71 125 3.41e-12 60.8
MS.gene003593.t1 MTR_4g005310 38.095 84 41 2 21 103 58 131 7.84e-12 59.3
MS.gene003593.t1 MTR_8g461210 40.260 77 45 1 4 80 14 89 8.10e-12 58.5
MS.gene003593.t1 MTR_0388s0020 44.776 67 33 2 15 78 65 130 2.89e-11 57.8
MS.gene003593.t1 MTR_7g118010 53.571 56 23 2 25 78 23 77 3.53e-11 56.6
MS.gene003593.t1 MTR_4g025830 50.877 57 24 2 25 78 79 134 4.50e-11 57.4
MS.gene003593.t1 MTR_3g092220 43.077 65 34 2 16 78 24 87 5.27e-11 56.2
MS.gene003593.t1 MTR_4g005320 46.667 60 31 1 25 84 70 128 6.23e-11 57.0
MS.gene003593.t1 MTR_4g072160 34.211 114 63 4 16 119 28 139 6.41e-11 56.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003593.t1 AT2G21200 62.500 72 25 2 7 76 2 73 8.31e-26 93.6
MS.gene003593.t1 AT4G38840 60.000 85 25 3 1 76 1 85 6.11e-25 91.7
MS.gene003593.t1 AT4G34800 54.321 81 30 2 1 78 1 77 7.38e-25 91.3
MS.gene003593.t1 AT4G34770 56.098 82 34 1 4 83 14 95 1.52e-24 90.9
MS.gene003593.t1 AT4G38825 56.757 74 28 2 7 76 2 75 4.98e-24 89.0
MS.gene003593.t1 AT2G21210 53.086 81 33 2 1 76 1 81 7.59e-24 89.0
MS.gene003593.t1 AT2G21210 53.086 81 33 2 1 76 68 148 3.23e-23 89.4
MS.gene003593.t1 AT4G38850 57.692 78 29 2 7 80 2 79 3.61e-23 87.0
MS.gene003593.t1 AT5G18020 54.545 77 33 2 1 76 1 76 1.05e-22 85.9
MS.gene003593.t1 AT3G03840 59.211 76 26 2 3 76 8 80 1.59e-22 85.5
MS.gene003593.t1 AT5G18080 55.844 77 32 2 1 76 1 76 2.38e-22 84.7
MS.gene003593.t1 AT4G34810 64.407 59 17 1 24 78 47 105 3.96e-22 85.1
MS.gene003593.t1 AT5G18060 54.667 75 29 2 7 76 2 76 4.29e-22 84.0
MS.gene003593.t1 AT3G03850 61.842 76 22 3 3 76 8 78 5.24e-22 84.0
MS.gene003593.t1 AT4G13790 48.193 83 41 1 1 81 1 83 5.69e-22 84.0
MS.gene003593.t1 AT5G18050 53.333 75 30 2 7 76 2 76 1.71e-21 82.8
MS.gene003593.t1 AT5G18030 54.795 73 30 2 7 76 2 74 2.07e-21 82.4
MS.gene003593.t1 AT3G03820 61.111 72 22 2 8 76 13 81 2.44e-21 82.4
MS.gene003593.t1 AT3G03830 55.263 76 25 2 3 75 8 77 1.72e-20 80.1
MS.gene003593.t1 AT5G18010 51.948 77 35 2 1 76 1 76 3.49e-20 79.3
MS.gene003593.t1 AT4G34790 62.500 56 19 1 25 78 39 94 1.22e-18 75.9
MS.gene003593.t1 AT4G38860 58.929 56 23 0 23 78 38 93 2.90e-18 74.7
MS.gene003593.t1 AT1G75580 60.714 56 21 1 23 78 42 96 1.55e-17 73.2
MS.gene003593.t1 AT2G21220 58.929 56 22 1 23 78 38 92 1.63e-17 72.8
MS.gene003593.t1 AT4G34780 61.404 57 19 1 23 76 26 82 4.83e-17 71.6
MS.gene003593.t1 AT4G34760 59.259 54 21 1 23 76 41 93 5.52e-17 71.6
MS.gene003593.t1 AT1G19830 57.143 56 23 1 23 78 46 100 4.57e-16 69.3
MS.gene003593.t1 AT4G36110 56.604 53 22 1 24 76 39 90 1.66e-14 65.1
MS.gene003593.t1 AT2G16580 50.847 59 28 1 23 81 42 99 2.10e-14 65.1
MS.gene003593.t1 AT2G18010 47.143 70 33 2 24 93 47 112 2.89e-14 64.7
MS.gene003593.t1 AT3G43120 47.692 65 33 1 14 78 68 131 1.98e-13 63.5
MS.gene003593.t1 AT1G75590 35.507 138 73 5 4 135 16 143 5.54e-13 62.4
MS.gene003593.t1 AT5G20810 54.545 55 24 1 24 78 78 131 2.88e-12 60.8
MS.gene003593.t1 AT3G20220 45.070 71 33 3 11 80 37 102 2.89e-12 59.7
MS.gene003593.t1 AT5G20810 51.667 60 28 1 17 76 71 129 3.13e-12 61.2
MS.gene003593.t1 AT1G19840 36.283 113 61 3 24 135 40 142 1.27e-11 58.9
MS.gene003593.t1 AT5G66260 49.123 57 28 1 25 81 34 89 1.66e-11 57.4
MS.gene003593.t1 AT3G09870 47.619 63 30 2 24 84 44 105 4.14e-11 56.6
MS.gene003593.t1 AT5G10990 42.466 73 41 1 24 96 40 111 5.38e-11 57.0
MS.gene003593.t1 AT3G12830 43.836 73 39 2 4 76 30 100 6.71e-11 56.6

Find 28 sgRNAs with CRISPR-Local

Find 97 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCAACAAGTCTTGAAATGA+AGG 0.342172 4.1:+30786700 None:intergenic
AGTCAAGCCGAGGAAGAGTT+TGG 0.367159 4.1:-30786679 MS.gene003593:CDS
TTACATGGAATTAGCAAAAC+AGG 0.383422 4.1:+30786197 None:intergenic
CTCTTAGAATAGAGTTTAAA+CGG 0.400936 4.1:+30786842 None:intergenic
GGTTTGTTCAAGTATGATAC+AGG 0.410295 4.1:+30786721 None:intergenic
CGTTTAAACTCTATTCTAAG+AGG 0.413979 4.1:-30786841 MS.gene003593:CDS
CGTGTATGTTGGAGAGAAAC+TGG 0.417120 4.1:-30786758 MS.gene003593:CDS
AAAATCAGTAGAAGTGAGAA+AGG 0.471464 4.1:-30786791 MS.gene003593:CDS
AAAATCTATGGGCGTTCCAA+AGG 0.472091 4.1:+30785076 None:intergenic
AAATCTATGGGCGTTCCAAA+GGG 0.529875 4.1:+30785077 None:intergenic
TACACAGCTAGATATCCCTT+TGG 0.531737 4.1:-30785092 MS.gene003593:CDS
ATCATATCCAAACTCTTCCT+CGG 0.532636 4.1:+30786672 None:intergenic
GGCTATGTTGCCGTGTATGT+TGG 0.537170 4.1:-30786769 MS.gene003593:CDS
GACATCTTCCGTACAAGGAA+TGG 0.540589 4.1:+30786630 None:intergenic
AAACTCTATTCTAAGAGGAT+CGG 0.556160 4.1:-30786836 MS.gene003593:CDS
GTTTGGATATGATCATCCCA+TGG 0.570301 4.1:-30786662 MS.gene003593:CDS
AAATCAGTAGAAGTGAGAAA+GGG 0.578349 4.1:-30786790 MS.gene003593:CDS
AGGAATGGTGAGGCCACCCA+TGG 0.587754 4.1:+30786645 None:intergenic
CAGTTTCTCTCCAACATACA+CGG 0.594831 4.1:+30786759 None:intergenic
GGATATGATCATCCCATGGG+TGG 0.600402 4.1:-30786658 MS.gene003593:CDS
AGACTTGTTGAGTCAAGCCG+AGG 0.633253 4.1:-30786689 MS.gene003593:CDS
TCTAGCTGTGTACGTAGGAG+AGG 0.638360 4.1:+30785103 None:intergenic
TTTGGATATGATCATCCCAT+GGG 0.641263 4.1:-30786661 MS.gene003593:CDS
GGAATGGTGAGGCCACCCAT+GGG 0.648249 4.1:+30786646 None:intergenic
CTTCCGTACAAGGAATGGTG+AGG 0.686019 4.1:+30786635 None:intergenic
GTTTGTTCAAGTATGATACA+GGG 0.688837 4.1:+30786722 None:intergenic
GGATATCTAGCTGTGTACGT+AGG 0.696914 4.1:+30785098 None:intergenic
ATGTTGGAGAGAAACTGGCG+CGG 0.774668 4.1:-30786753 MS.gene003593:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTCATATTTATAAATAAG+GGG - chr4.1:30786473-30786492 MS.gene003593:intron 10.0%
!! ATAATTTAGCCAAATTAATT+AGG - chr4.1:30785793-30785812 MS.gene003593:intron 15.0%
!!! AAGAATTTTAATTACTTACA+TGG + chr4.1:30785752-30785771 None:intergenic 15.0%
!!! AGTTTTTCAAATTATTTTGA+AGG + chr4.1:30786047-30786066 None:intergenic 15.0%
!! TATCTCTAATACTAGATAAT+TGG - chr4.1:30786200-30786219 MS.gene003593:intron 20.0%
!! TTTGCTTCAAATATGATATA+GGG - chr4.1:30786786-30786805 MS.gene003593:CDS 20.0%
!!! AACAAATTTGCTACAAAAAT+TGG - chr4.1:30786598-30786617 MS.gene003593:intron 20.0%
!!! ATCTTTGTATTGATGATAAA+AGG - chr4.1:30786118-30786137 MS.gene003593:intron 20.0%
!!! GTTGTTTTGTAAAAAAACAA+TGG + chr4.1:30786267-30786286 None:intergenic 20.0%
!!! TTTGAGACAATTTTCATTAT+AGG - chr4.1:30785396-30785415 MS.gene003593:CDS 20.0%
!!! TTTTGCTTCAAATATGATAT+AGG - chr4.1:30786785-30786804 MS.gene003593:CDS 20.0%
!!! TTTTTTTACAAAACAACAAC+AGG - chr4.1:30786270-30786289 MS.gene003593:intron 20.0%
! AAACTAGATGTTATGAAACA+TGG - chr4.1:30786062-30786081 MS.gene003593:intron 25.0%
! AAAGAACTAACTATTGTAGT+AGG + chr4.1:30785641-30785660 None:intergenic 25.0%
! AATGGAGAAAATTTACTATC+TGG - chr4.1:30785457-30785476 MS.gene003593:intron 25.0%
! ACGAATTTACTTCTAATGTT+TGG - chr4.1:30785999-30786018 MS.gene003593:intron 25.0%
! CATATAATTTCTTCCTTGAA+TGG - chr4.1:30785330-30785349 MS.gene003593:intron 25.0%
! CATATTTATAAATAAGGGGA+AGG - chr4.1:30786477-30786496 MS.gene003593:intron 25.0%
! CTCTTAGAATAGAGTTTAAA+CGG + chr4.1:30785092-30785111 None:intergenic 25.0%
! GTCATTAAACAAGAAAAAAG+AGG + chr4.1:30785507-30785526 None:intergenic 25.0%
! GTTATTTATTCTTCATAAGC+AGG + chr4.1:30785596-30785615 None:intergenic 25.0%
! TATTTAACCAAACTAACCAA+TGG + chr4.1:30785852-30785871 None:intergenic 25.0%
! TCAAGGAAGAAATTATATGT+TGG + chr4.1:30785329-30785348 None:intergenic 25.0%
! TGAGTGAATTATTCTACTAT+AGG - chr4.1:30786152-30786171 MS.gene003593:intron 25.0%
! TTAGCCAAATTAATTAGGTT+CGG - chr4.1:30785798-30785817 MS.gene003593:intron 25.0%
! TTGAATCAACTTCAAACTAT+AGG + chr4.1:30785907-30785926 None:intergenic 25.0%
!! CAAAAGAGAAAATCTTTTGA+AGG - chr4.1:30786570-30786589 MS.gene003593:intron 25.0%
!! GTAAATATTACCCTTTTCAT+GGG - chr4.1:30785433-30785452 MS.gene003593:intron 25.0%
!! TGTAAATATTACCCTTTTCA+TGG - chr4.1:30785432-30785451 MS.gene003593:intron 25.0%
!!! AGTTTCTATTTTTTTGCAAC+AGG - chr4.1:30786371-30786390 MS.gene003593:intron 25.0%
!!! GTTTCTATTTTTTTGCAACA+GGG - chr4.1:30786372-30786391 MS.gene003593:intron 25.0%
!!! TCTTTTGTTGTTATCATACT+TGG + chr4.1:30786557-30786576 None:intergenic 25.0%
!!! TTTCTATTTTTTTGCAACAG+GGG - chr4.1:30786373-30786392 MS.gene003593:intron 25.0%
AAAATCAGTAGAAGTGAGAA+AGG - chr4.1:30785140-30785159 MS.gene003593:intron 30.0%
AAATCAGTAGAAGTGAGAAA+GGG - chr4.1:30785141-30785160 MS.gene003593:intron 30.0%
AATTGTAGGTGACAAGTAAA+TGG - chr4.1:30786234-30786253 MS.gene003593:intron 30.0%
ATTTGAGTATGATCATCCAA+TGG + chr4.1:30786729-30786748 None:intergenic 30.0%
CAAATTTCACTAGCTATAGT+TGG - chr4.1:30785555-30785574 MS.gene003593:intron 30.0%
CGTTTAAACTCTATTCTAAG+AGG - chr4.1:30785090-30785109 MS.gene003593:CDS 30.0%
GTTTGTTCAAGTATGATACA+GGG + chr4.1:30785212-30785231 None:intergenic 30.0%
TAAGATTCATAGTCCATTCA+AGG + chr4.1:30785346-30785365 None:intergenic 30.0%
TTACATGGAATTAGCAAAAC+AGG + chr4.1:30785737-30785756 None:intergenic 30.0%
TTTACTTCTAATGTTTGGCA+TGG - chr4.1:30786004-30786023 MS.gene003593:intron 30.0%
TTTGAGTATGATCATCCAAT+GGG + chr4.1:30786728-30786747 None:intergenic 30.0%
! AAACTCTATTCTAAGAGGAT+CGG - chr4.1:30785095-30785114 MS.gene003593:CDS 30.0%
!!! TTCTATTTTTTTGCAACAGG+GGG - chr4.1:30786374-30786393 MS.gene003593:intron 30.0%
AAACTAACCAATGGAAGCAA+AGG + chr4.1:30785843-30785862 None:intergenic 35.0%
AAGAAAACAACTTCTCTGCA+AGG - chr4.1:30786689-30786708 MS.gene003593:CDS 35.0%
ACTCAAATTGTTCCTCACTT+TGG - chr4.1:30786741-30786760 MS.gene003593:CDS 35.0%
ATCATATCCAAACTCTTCCT+CGG + chr4.1:30785262-30785281 None:intergenic 35.0%
CTCAACAAGTCTTGAAATGA+AGG + chr4.1:30785234-30785253 None:intergenic 35.0%
GACTATGAATCTTACACTGT+AGG - chr4.1:30785352-30785371 MS.gene003593:intron 35.0%
TAGGCCGAACCTAATTAATT+TGG + chr4.1:30785805-30785824 None:intergenic 35.0%
TTCTCCATTTCCCATGAAAA+GGG + chr4.1:30785446-30785465 None:intergenic 35.0%
TTTCTCCATTTCCCATGAAA+AGG + chr4.1:30785447-30785466 None:intergenic 35.0%
TTTGGATATGATCATCCCAT+GGG - chr4.1:30785270-30785289 MS.gene003593:intron 35.0%
! ATTACCCTTTTCATGGGAAA+TGG - chr4.1:30785439-30785458 MS.gene003593:intron 35.0%
! GGTTTGTTCAAGTATGATAC+AGG + chr4.1:30785213-30785232 None:intergenic 35.0%
! TTTGCTTCCATTGGTTAGTT+TGG - chr4.1:30785842-30785861 MS.gene003593:intron 35.0%
!!! CAAAACAGGTTTTGTTGCAT+AGG + chr4.1:30785723-30785742 None:intergenic 35.0%
!!! TCTATTTTTTTGCAACAGGG+GGG - chr4.1:30786375-30786394 MS.gene003593:intron 35.0%
AAAGAGCCATAAGATACAGC+AGG + chr4.1:30785942-30785961 None:intergenic 40.0%
AAGAGCCATAAGATACAGCA+GGG + chr4.1:30785941-30785960 None:intergenic 40.0%
CAGTTTCTCTCCAACATACA+CGG + chr4.1:30785175-30785194 None:intergenic 40.0%
GTTATGAAACATGGCATCCA+TGG - chr4.1:30786071-30786090 MS.gene003593:intron 40.0%
GTTGCATAGGAAGATTTAGC+TGG + chr4.1:30785710-30785729 None:intergenic 40.0%
GTTTGGATATGATCATCCCA+TGG - chr4.1:30785269-30785288 MS.gene003593:intron 40.0%
TAATGTTTGGCATGGAGTTC+TGG - chr4.1:30786012-30786031 MS.gene003593:intron 40.0%
TACACAGCTAGATATCCCTT+TGG - chr4.1:30786839-30786858 MS.gene003593:CDS 40.0%
! AAGGCAGTTTCTGAAGACAT+AGG + chr4.1:30785824-30785843 None:intergenic 40.0%
! TGGCAACTGTCTGAAATTGT+AGG - chr4.1:30786220-30786239 MS.gene003593:CDS 40.0%
AACTGCCCTGCTGTATCTTA+TGG - chr4.1:30785933-30785952 MS.gene003593:intron 45.0%
ACAATTGCTGAGCCAAAGTG+AGG + chr4.1:30786756-30786775 None:intergenic 45.0%
ATGGCATCCATGGAAGGAAT+GGG - chr4.1:30786081-30786100 MS.gene003593:intron 45.0%
CGTGTATGTTGGAGAGAAAC+TGG - chr4.1:30785173-30785192 MS.gene003593:intron 45.0%
GAAACTGCCTTTGCTTCCAT+TGG - chr4.1:30785833-30785852 MS.gene003593:intron 45.0%
GACATCTTCCGTACAAGGAA+TGG + chr4.1:30785304-30785323 None:intergenic 45.0%
GAGTATGATCATCCAATGGG+TGG + chr4.1:30786725-30786744 None:intergenic 45.0%
GGATATCTAGCTGTGTACGT+AGG + chr4.1:30786836-30786855 None:intergenic 45.0%
TGAAACATGGCATCCATGGA+AGG - chr4.1:30786075-30786094 MS.gene003593:intron 45.0%
TGGAAGACATCTTCCGTACA+AGG + chr4.1:30785309-30785328 None:intergenic 45.0%
! CACTTTGGCTCAGCAATTGT+TGG - chr4.1:30786756-30786775 MS.gene003593:CDS 45.0%
! GTTTTCTCCCATTCCTTCCA+TGG + chr4.1:30786091-30786110 None:intergenic 45.0%
!!! ATTTTCATATTTATAAATAA+GGG - chr4.1:30786472-30786491 MS.gene003593:intron 5.0%
!!! TATTTTCATATTTATAAATA+AGG - chr4.1:30786471-30786490 MS.gene003593:intron 5.0%
AGTCAAGCCGAGGAAGAGTT+TGG - chr4.1:30785252-30785271 MS.gene003593:intron 50.0%
ATGTTGGAGAGAAACTGGCG+CGG - chr4.1:30785178-30785197 MS.gene003593:intron 50.0%
CATGGCATCCATGGAAGGAA+TGG - chr4.1:30786080-30786099 MS.gene003593:intron 50.0%
CTTCCGTACAAGGAATGGTG+AGG + chr4.1:30785299-30785318 None:intergenic 50.0%
GGAATAGTGAGACCACCCAT+TGG - chr4.1:30786710-30786729 MS.gene003593:CDS 50.0%
GGATATGATCATCCCATGGG+TGG - chr4.1:30785273-30785292 MS.gene003593:intron 50.0%
GGCTATGTTGCCGTGTATGT+TGG - chr4.1:30785162-30785181 MS.gene003593:intron 50.0%
TCTAGCTGTGTACGTAGGAG+AGG + chr4.1:30786831-30786850 None:intergenic 50.0%
TGGCCTCACCATTCCTTGTA+CGG - chr4.1:30785293-30785312 MS.gene003593:intron 50.0%
!! AGACTTGTTGAGTCAAGCCG+AGG - chr4.1:30785242-30785261 MS.gene003593:intron 50.0%
AGGAATGGTGAGGCCACCCA+TGG + chr4.1:30785289-30785308 None:intergenic 60.0%
GGAATGGTGAGGCCACCCAT+GGG + chr4.1:30785288-30785307 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 30785081 30786872 30785081 ID=MS.gene003593
chr4.1 mRNA 30785081 30786872 30785081 ID=MS.gene003593.t1;Parent=MS.gene003593
chr4.1 exon 30786644 30786872 30786644 ID=MS.gene003593.t1.exon1;Parent=MS.gene003593.t1
chr4.1 CDS 30786644 30786872 30786644 ID=cds.MS.gene003593.t1;Parent=MS.gene003593.t1
chr4.1 exon 30786201 30786242 30786201 ID=MS.gene003593.t1.exon2;Parent=MS.gene003593.t1
chr4.1 CDS 30786201 30786242 30786201 ID=cds.MS.gene003593.t1;Parent=MS.gene003593.t1
chr4.1 exon 30785362 30785432 30785362 ID=MS.gene003593.t1.exon3;Parent=MS.gene003593.t1
chr4.1 CDS 30785362 30785432 30785362 ID=cds.MS.gene003593.t1;Parent=MS.gene003593.t1
chr4.1 exon 30785081 30785146 30785081 ID=MS.gene003593.t1.exon4;Parent=MS.gene003593.t1
chr4.1 CDS 30785081 30785146 30785081 ID=cds.MS.gene003593.t1;Parent=MS.gene003593.t1
Gene Sequence

>MS.gene003593

ATGGGTTTCCGTTTAAACTCTATTCTAAGAGGATCGGTAACAGCTAGACAAGCAACATCAAAATCAGTAGAAGTGAGAAAGGGCTATGTTGCCGTGTATGTTGGAGAGAAACTGGCGCGGTTTGTAGTCCCTGTATCATACTTGAACAAACCTTCATTTCAAGACTTGTTGAGTCAAGCCGAGGAAGAGTTTGGATATGATCATCCCATGGGTGGCCTCACCATTCCTTGTACGGAAGATGTCTTCCAACATATAATTTCTTCCTTGAATGGACTATGAATCTTACACTGTAGGATACTCACATAGACTAGATTATTTGAGACAATTTTCATTATAGGCATCTTTTTTAATTGTAAATATTACCCTTTTCATGGGAAATGGAGAAAATTTACTATCTGGATAACAAATTCAAAAAATTACTGTCCTCTTTTTTCTTGTTTAATGACACATTGAGCTCATTTACTTTATCAGTTACAAATTTCACTAGCTATAGTTGGTTCTTTAAATTTAAGCCTGCTTATGAAGAATAAATAACTTTGCTGATTTAAAAGATAAAGCCTACTACAATAGTTAGTTCTTTAAGTCTGTTGAAATAAGATAATCTGTTTCTTTTACTTCTGTAAAATCCAGCTAAATCTTCCTATGCAACAAAACCTGTTTTGCTAATTCCATGTAAGTAATTAAAATTCTTAAACAAAATCAATAACTATTGATAATTTAGCCAAATTAATTAGGTTCGGCCTATGTCTTCAGAAACTGCCTTTGCTTCCATTGGTTAGTTTGGTTAAATATTTCACATTTATTCAGAAGTTCTCTTTCAACTCCTATAGTTTGAAGTTGATTCAAATTTCAAACTGCCCTGCTGTATCTTATGGCTCTTTAGTCTTTAACTATTTAATTTTCTCAAAACATAAAATCACGAATTTACTTCTAATGTTTGGCATGGAGTTCTGGTAATTTTCTCCTTCAAAATAATTTGAAAAACTAGATGTTATGAAACATGGCATCCATGGAAGGAATGGGAGAAAACTCAAATAATCTTTGTATTGATGATAAAAGGAATATTACAAATGAGTGAATTATTCTACTATAGGACTAATACTACTTGTAACTAAACTATATCTCTAATACTAGATAATTGGCAACTGTCTGAAATTGTAGGTGACAAGTAAATGGAGATAAACCATTGTTTTTTTACAAAACAACAACAGGATCTATGACTAAAAATACAAAGAAAACTACAAATAAATCAATATAGTTAGATTCAGTGTTTTTCTTGAAGTTATTTTAAGTTTCTATTTTTTTGCAACAGGGGGGATTCGATTTTATATTTGAAGTGAATGAATTTTTCATTTTATCAGAGAGATTATAAACTGAAAATTTAGCAATCTATTTTCATATTTATAAATAAGGGGAAGGAATAGAGAAATAATGTAATAGTGTTTAAAAATTTCTCATATGAATGTTTTACATCCAAGTATGATAACAACAAAAGAGAAAATCTTTTGAAGGTAAGAAACAAATTTGCTACAAAAATTGGTCAAATATCTATGTCAATCTTCTAATATCTTCAGAGATCTACTTCAATTTAAGTTAGAAGTGATGTCTAAGAAAACAACTTCTCTGCAAGGAATAGTGAGACCACCCATTGGATGATCATACTCAAATTGTTCCTCACTTTGGCTCAGCAATTGTTGGAATGATTTTTGCTTCAAATATGATATAGGGATCACAAATCTCTTCATGTCCTCTCCTACGTACACAGCTAGATATCCCTTTGGAACGCCCATAG
Protein sequence

>MS.gene003593.t1

MGFRLNSILRGSVTARQATSKSVEVRKGYVAVYVGEKLARFVVPVSYLNKPSFQDLLSQAEEEFGYDHPMGGLTIPSKSSYATKPVLLIPLFKNFSYECFTSKYDNNKRENLLKGSQISSCPLLRTQLDIPLERP