AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene031200

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031200.t1 MTR_3g084150 89.130 92 10 0 1 92 1 92 6.88e-60 177
MS.gene031200.t1 MTR_8g461410 88.043 92 11 0 1 92 1 92 1.34e-59 177
MS.gene031200.t1 MTR_3g084180 88.043 92 11 0 1 92 1 92 6.98e-59 175
MS.gene031200.t1 MTR_3g110042 94.253 87 5 0 1 87 1 87 9.15e-58 172
MS.gene031200.t1 MTR_3g084170 86.022 93 11 2 1 92 1 92 9.92e-55 164
MS.gene031200.t1 MTR_3g084240 84.946 93 12 2 1 92 1 92 1.11e-53 162
MS.gene031200.t1 MTR_4g050700 81.720 93 16 1 1 92 1 93 2.36e-53 161
MS.gene031200.t1 MTR_2g012410 81.720 93 16 1 1 92 1 93 2.36e-53 161
MS.gene031200.t1 MTR_3g084220 83.871 93 13 2 1 92 1 92 5.00e-53 160
MS.gene031200.t1 MTR_3g110032 79.570 93 18 1 1 92 1 93 8.01e-52 157
MS.gene031200.t1 MTR_3g110038 81.720 93 15 2 1 92 1 92 3.27e-51 155
MS.gene031200.t1 MTR_3g117630 84.884 86 13 0 7 92 7 92 6.32e-51 155
MS.gene031200.t1 MTR_4g072500 75.000 92 23 0 1 92 1 92 1.76e-48 149
MS.gene031200.t1 MTR_3g084260 76.344 93 21 1 1 92 1 93 5.50e-48 147
MS.gene031200.t1 MTR_4g072880 72.043 93 25 1 1 92 1 93 1.82e-47 146
MS.gene031200.t1 MTR_3g084250 72.826 92 25 0 1 92 1 92 4.20e-47 145
MS.gene031200.t1 MTR_4g072250 75.269 93 21 2 1 92 1 92 7.35e-47 145
MS.gene031200.t1 MTR_4g072860 74.194 93 23 1 1 92 1 93 8.01e-47 145
MS.gene031200.t1 MTR_4g072480 72.826 92 25 0 1 92 1 92 8.21e-47 144
MS.gene031200.t1 MTR_4g072680 72.826 92 25 0 1 92 1 92 2.41e-46 143
MS.gene031200.t1 MTR_4g072270 70.968 93 26 1 1 92 1 93 3.85e-46 143
MS.gene031200.t1 MTR_4g072310 70.968 93 26 1 1 92 1 93 4.89e-46 142
MS.gene031200.t1 MTR_4g072570 75.000 92 23 0 1 92 1 92 6.18e-46 142
MS.gene031200.t1 MTR_4g072330 72.222 90 23 1 1 90 1 88 8.74e-46 142
MS.gene031200.t1 MTR_4g072620 70.652 92 27 0 1 92 1 92 1.28e-45 142
MS.gene031200.t1 MTR_4g072530 72.222 90 25 0 1 90 1 90 1.43e-45 141
MS.gene031200.t1 MTR_4g072640 73.333 90 24 0 1 90 1 90 1.89e-45 141
MS.gene031200.t1 MTR_4g072730 71.429 91 26 0 1 91 1 91 2.33e-45 141
MS.gene031200.t1 MTR_4g072460 70.652 92 27 0 1 92 1 92 1.10e-44 139
MS.gene031200.t1 MTR_4g072490 73.626 91 17 2 1 90 1 85 1.44e-44 139
MS.gene031200.t1 MTR_4g072870 70.330 91 26 1 1 90 1 91 2.45e-44 138
MS.gene031200.t1 MTR_4g072240 71.429 91 25 1 1 90 1 91 4.78e-44 137
MS.gene031200.t1 MTR_3g084210 75.000 92 23 0 1 92 1 92 7.00e-44 137
MS.gene031200.t1 MTR_4g072370 72.222 90 23 1 1 90 1 88 7.44e-44 137
MS.gene031200.t1 MTR_4g072230 72.043 93 23 2 1 92 1 91 1.33e-43 136
MS.gene031200.t1 MTR_4g072740 72.222 90 20 1 1 90 1 85 2.37e-43 135
MS.gene031200.t1 MTR_4g072610 72.222 90 19 1 1 90 1 84 4.31e-43 135
MS.gene031200.t1 MTR_4g072465 68.889 90 24 1 1 90 1 86 1.17e-42 134
MS.gene031200.t1 MTR_4g072830 74.713 87 20 1 1 87 1 85 1.27e-42 134
MS.gene031200.t1 MTR_4g072300 69.231 91 27 1 1 90 1 91 2.08e-42 133
MS.gene031200.t1 MTR_4g072220 67.742 93 29 1 1 92 1 93 2.66e-42 133
MS.gene031200.t1 MTR_4g072850 74.468 94 22 1 1 92 1 94 4.03e-42 133
MS.gene031200.t1 MTR_3g084190 72.826 92 25 0 1 92 1 92 4.14e-42 133
MS.gene031200.t1 MTR_4g072650 72.222 90 19 1 1 90 1 84 4.51e-42 132
MS.gene031200.t1 MTR_4g072630 68.889 90 24 1 1 90 1 86 9.95e-42 132
MS.gene031200.t1 MTR_4g072260 65.591 93 31 1 1 92 1 93 1.15e-41 132
MS.gene031200.t1 MTR_8g461420 73.333 90 24 0 1 90 1 90 1.23e-41 132
MS.gene031200.t1 MTR_4g072890 67.033 91 29 1 1 90 1 91 1.31e-41 131
MS.gene031200.t1 MTR_4g072470 70.000 90 22 1 1 90 1 85 2.30e-41 130
MS.gene031200.t1 MTR_3g084230 73.333 90 24 0 1 90 1 90 3.80e-41 130
MS.gene031200.t1 MTR_4g072580 71.429 91 19 2 1 90 1 85 3.82e-41 130
MS.gene031200.t1 MTR_4g072660 67.391 92 25 1 1 92 1 87 6.61e-41 129
MS.gene031200.t1 MTR_5g008360 75.949 79 19 0 14 92 5 83 7.83e-41 129
MS.gene031200.t1 MTR_3g084160 70.652 92 27 0 1 92 1 92 8.94e-41 129
MS.gene031200.t1 MTR_4g072540 73.333 90 20 1 1 90 1 86 1.09e-40 129
MS.gene031200.t1 MTR_3g110045 74.444 90 23 0 3 92 62 151 4.47e-40 129
MS.gene031200.t1 MTR_4g072820 69.231 91 21 2 1 90 1 85 1.16e-39 126
MS.gene031200.t1 MTR_4g072670 68.889 90 24 1 1 90 1 86 1.26e-39 126
MS.gene031200.t1 MTR_4g072720 71.111 90 22 1 1 90 1 86 1.58e-39 126
MS.gene031200.t1 MTR_4g072590 71.111 90 21 1 1 90 1 85 1.83e-39 126
MS.gene031200.t1 MTR_4g072600 69.231 91 21 2 1 90 1 85 3.83e-39 125
MS.gene031200.t1 MTR_4g072510 70.000 90 23 1 1 90 1 86 2.10e-38 123
MS.gene031200.t1 MTR_3g084200 80.822 73 12 2 1 72 1 72 7.21e-38 122
MS.gene031200.t1 MTR_5g008370 65.934 91 31 0 1 91 1 91 8.73e-38 123
MS.gene031200.t1 MTR_4g072940 72.826 92 23 1 3 92 33 124 9.10e-38 123
MS.gene031200.t1 MTR_4g072380 69.231 91 22 2 1 90 1 86 1.48e-37 121
MS.gene031200.t1 MTR_4g072690 71.111 90 21 1 1 90 1 85 7.30e-37 119
MS.gene031200.t1 MTR_4g072450 70.000 90 22 1 1 90 1 85 1.68e-36 118
MS.gene031200.t1 MTR_5g008350 70.000 80 17 2 1 79 1 74 5.22e-35 114
MS.gene031200.t1 MTR_4g072550 68.493 73 23 0 20 92 31 103 3.33e-33 110
MS.gene031200.t1 MTR_4g072980 53.684 95 41 2 1 92 1 95 9.42e-33 109
MS.gene031200.t1 MTR_4g072560 51.020 98 40 2 1 90 1 98 7.34e-32 107
MS.gene031200.t1 MTR_4g072770 58.889 90 27 1 1 90 1 80 3.16e-31 105
MS.gene031200.t1 MTR_4g072750 67.500 80 24 1 1 78 1 80 5.31e-31 104
MS.gene031200.t1 MTR_4g072910 57.831 83 35 0 8 90 18 100 6.73e-31 105
MS.gene031200.t1 MTR_4g072290 56.989 93 25 2 1 92 1 79 1.21e-29 101
MS.gene031200.t1 MTR_8g461400 59.140 93 10 3 1 92 1 66 8.25e-29 98.6
MS.gene031200.t1 MTR_3g117640 62.319 69 25 1 24 91 33 101 1.22e-28 99.4
MS.gene031200.t1 MTR_4g072280 64.000 75 26 1 1 74 1 75 1.26e-27 96.3
MS.gene031200.t1 MTR_3g110035 65.152 66 23 0 27 92 73 138 2.21e-27 97.4
MS.gene031200.t1 MTR_4g072930 47.872 94 43 2 3 90 17 110 4.40e-24 88.2
MS.gene031200.t1 MTR_6g077850 53.731 67 31 0 24 90 33 99 9.98e-24 87.0
MS.gene031200.t1 MTR_3g113310 46.512 86 39 2 8 87 19 103 3.21e-23 85.9
MS.gene031200.t1 MTR_3g117610 56.923 65 27 1 23 87 43 106 8.99e-23 84.7
MS.gene031200.t1 MTR_4g072190 48.750 80 38 2 8 87 21 97 3.97e-22 82.8
MS.gene031200.t1 MTR_4g072840 50.575 87 13 2 1 87 1 57 1.08e-21 80.5
MS.gene031200.t1 MTR_8g076040 47.297 74 38 1 14 87 30 102 5.81e-19 74.7
MS.gene031200.t1 MTR_2g043960 54.839 62 27 1 24 85 75 135 7.47e-19 76.3
MS.gene031200.t1 MTR_5g021820 51.562 64 30 1 24 87 39 101 8.19e-19 74.3
MS.gene031200.t1 MTR_4g124750 53.226 62 28 1 24 85 68 128 6.02e-18 73.6
MS.gene031200.t1 MTR_1g009150 50.704 71 34 1 15 85 61 130 2.14e-17 72.8
MS.gene031200.t1 MTR_4g005310 50.000 66 32 1 21 85 58 123 7.24e-17 70.9
MS.gene031200.t1 MTR_4g005320 54.098 61 27 1 25 85 70 129 1.82e-16 70.1
MS.gene031200.t1 MTR_1g094750 44.318 88 45 2 1 85 23 109 2.81e-16 68.6
MS.gene031200.t1 MTR_3g109160 52.239 67 31 1 25 91 69 134 3.64e-15 67.0
MS.gene031200.t1 MTR_1g024085 49.254 67 33 1 16 82 28 93 8.53e-15 65.5
MS.gene031200.t1 MTR_7g118010 47.692 65 31 2 25 87 23 86 2.29e-14 63.2
MS.gene031200.t1 MTR_8g461210 52.542 59 27 1 24 82 34 91 2.55e-14 63.5
MS.gene031200.t1 MTR_3g105900 67.442 43 14 0 22 64 1169 1211 3.21e-14 66.6
MS.gene031200.t1 MTR_1g061570 42.169 83 44 2 9 91 6 84 5.09e-14 62.8
MS.gene031200.t1 MTR_4g072160 52.381 63 27 2 24 85 36 96 5.27e-14 63.2
MS.gene031200.t1 MTR_3g092220 40.476 84 44 4 1 82 12 91 9.73e-14 62.0
MS.gene031200.t1 MTR_4g025830 46.875 64 30 2 25 85 79 141 1.68e-13 62.4
MS.gene031200.t1 MTR_0388s0020 43.038 79 40 3 10 85 61 137 2.08e-13 62.0
MS.gene031200.t1 MTR_8g026730 43.077 65 34 2 25 87 27 90 1.56e-12 58.5
MS.gene031200.t1 MTR_7g101740 45.312 64 34 1 24 87 39 101 2.15e-12 58.5
MS.gene031200.t1 MTR_1g063950 43.548 62 34 1 24 85 47 107 2.81e-12 58.2
MS.gene031200.t1 MTR_7g405740 39.286 84 46 3 6 85 44 126 3.49e-12 58.9
MS.gene031200.t1 MTR_4g021810 50.847 59 27 2 25 82 26 83 4.29e-12 57.4
MS.gene031200.t1 MTR_3g098970 32.895 76 50 1 17 92 45 119 1.07e-11 57.4
MS.gene031200.t1 MTR_2g066830 43.103 58 32 1 25 82 58 114 3.58e-11 55.8
MS.gene031200.t1 MTR_3g097120 42.667 75 34 2 19 85 3 76 3.99e-11 55.1
MS.gene031200.t1 MTR_8g101520 36.364 88 51 2 7 90 28 114 5.30e-11 55.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031200.t1 AT4G38840 54.545 99 36 2 1 90 1 99 7.30e-33 110
MS.gene031200.t1 AT5G18020 59.211 76 30 1 16 90 15 90 3.73e-31 105
MS.gene031200.t1 AT5G18060 59.211 76 30 1 16 90 15 90 1.18e-30 103
MS.gene031200.t1 AT5G18050 60.274 73 28 1 16 87 15 87 1.74e-30 103
MS.gene031200.t1 AT4G38825 58.667 75 30 1 17 90 15 89 8.35e-30 102
MS.gene031200.t1 AT5G18080 58.025 81 26 2 8 87 14 87 1.55e-29 101
MS.gene031200.t1 AT4G34800 56.322 87 34 2 7 89 2 88 2.00e-29 101
MS.gene031200.t1 AT2G21200 66.667 66 21 1 19 83 15 80 2.40e-29 100
MS.gene031200.t1 AT5G18030 59.722 72 28 1 20 90 17 88 4.39e-29 100
MS.gene031200.t1 AT4G34770 61.842 76 27 1 18 91 28 103 2.46e-28 98.6
MS.gene031200.t1 AT4G34810 62.319 69 22 1 24 88 47 115 3.56e-28 98.6
MS.gene031200.t1 AT5G18010 55.556 81 28 2 8 87 14 87 3.97e-28 97.8
MS.gene031200.t1 AT2G21210 56.757 74 30 1 19 90 22 95 9.38e-28 97.1
MS.gene031200.t1 AT4G38850 62.687 67 22 1 24 87 20 86 9.80e-28 96.7
MS.gene031200.t1 AT3G03820 57.143 77 30 1 18 91 20 96 1.12e-27 96.7
MS.gene031200.t1 AT3G03840 58.904 73 28 1 21 91 23 95 1.15e-27 96.7
MS.gene031200.t1 AT3G03850 63.235 68 23 1 26 91 26 93 3.86e-27 95.5
MS.gene031200.t1 AT2G21210 56.757 74 30 1 19 90 89 162 7.71e-27 96.7
MS.gene031200.t1 AT3G03830 57.746 71 27 1 20 87 19 89 5.40e-25 89.7
MS.gene031200.t1 AT4G13790 50.000 82 34 2 8 87 13 89 3.95e-24 87.8
MS.gene031200.t1 AT4G34790 59.091 66 25 1 25 88 39 104 8.22e-24 87.4
MS.gene031200.t1 AT4G38860 56.923 65 28 0 23 87 38 102 1.66e-23 86.7
MS.gene031200.t1 AT4G34780 58.333 72 27 2 22 90 25 96 4.67e-23 85.5
MS.gene031200.t1 AT1G75580 49.367 79 39 1 9 87 28 105 9.69e-23 84.7
MS.gene031200.t1 AT2G21220 55.385 65 28 1 23 87 38 101 8.71e-22 82.0
MS.gene031200.t1 AT4G34760 53.846 65 29 1 23 87 41 104 9.64e-21 79.7
MS.gene031200.t1 AT4G36110 46.429 84 42 2 8 89 21 103 3.56e-20 78.2
MS.gene031200.t1 AT3G43120 48.611 72 36 1 14 85 68 138 1.26e-19 78.2
MS.gene031200.t1 AT1G19830 43.617 94 40 2 8 89 19 111 2.55e-19 76.3
MS.gene031200.t1 AT2G18010 51.515 66 31 1 24 89 47 111 3.96e-19 75.5
MS.gene031200.t1 AT2G16580 52.308 65 30 1 23 87 42 105 4.44e-19 75.5
MS.gene031200.t1 AT5G20810 54.839 62 27 1 24 85 78 138 3.11e-18 74.7
MS.gene031200.t1 AT5G20810 50.725 69 33 1 17 85 71 138 5.69e-18 74.7
MS.gene031200.t1 AT3G20220 46.053 76 39 2 15 89 37 111 6.19e-18 72.8
MS.gene031200.t1 AT5G66260 45.882 85 43 2 8 90 15 98 4.64e-17 70.1
MS.gene031200.t1 AT1G75590 54.237 59 26 1 24 82 41 98 4.15e-16 68.9
MS.gene031200.t1 AT5G10990 52.542 59 27 1 24 82 40 97 3.69e-15 66.2
MS.gene031200.t1 AT4G34750 39.241 79 47 1 9 87 21 98 4.58e-15 66.2
MS.gene031200.t1 AT4G34750 39.241 79 47 1 9 87 21 98 4.58e-15 66.2
MS.gene031200.t1 AT1G19840 54.237 59 26 1 24 82 40 97 5.03e-15 66.2
MS.gene031200.t1 AT4G31320 48.101 79 33 3 8 85 68 139 6.03e-15 66.6
MS.gene031200.t1 AT2G46690 41.026 78 42 2 17 91 16 92 1.17e-14 64.3
MS.gene031200.t1 AT2G24400 49.367 79 32 3 8 85 52 123 1.31e-14 65.5
MS.gene031200.t1 AT3G51200 46.377 69 35 2 24 91 38 105 1.79e-14 63.5
MS.gene031200.t1 AT3G61900 39.286 84 47 2 10 90 15 97 1.17e-13 62.4
MS.gene031200.t1 AT3G12830 38.202 89 50 2 6 90 27 114 1.73e-13 61.6
MS.gene031200.t1 AT2G37030 46.032 63 33 1 24 86 48 109 6.88e-13 60.1
MS.gene031200.t1 AT3G53250 46.032 63 30 1 24 86 51 109 1.12e-12 59.3
MS.gene031200.t1 AT3G53250 46.032 63 30 1 24 86 82 140 2.05e-12 59.3
MS.gene031200.t1 AT4G00880 47.692 65 31 2 25 87 28 91 6.33e-12 57.4
MS.gene031200.t1 AT1G56150 43.939 66 36 1 25 90 38 102 6.46e-12 57.0
MS.gene031200.t1 AT4G12410 45.714 70 30 3 25 87 76 144 6.91e-12 58.2
MS.gene031200.t1 AT2G45210 48.214 56 25 2 25 77 79 133 8.38e-12 57.8
MS.gene031200.t1 AT3G09870 45.455 66 33 2 15 78 35 99 9.13e-12 56.6
MS.gene031200.t1 AT4G09530 44.444 72 35 3 14 82 19 88 9.27e-12 56.6
MS.gene031200.t1 AT5G53590 38.889 72 38 2 24 90 45 115 1.61e-11 57.0
MS.gene031200.t1 AT3G03847 56.604 53 21 2 33 85 80 130 5.85e-11 55.1

Find 0 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAGAAAACATCTTCTTTGCA+AGG + 21207:22346-22365 None:intergenic 30.0%
ATTAGACGAACTTCATTTAC+AGG - 21207:22139-22158 MS.gene031200:CDS 30.0%
TGTAAATGAAGTTCGTCTAA+TGG + 21207:22140-22159 None:intergenic 30.0%
!!! ACAAGTTTTGAAATGAAGCT+TGG + 21207:22267-22286 None:intergenic 30.0%
AAAGTTGTGAATGTGCCAAA+GGG - 21207:22178-22197 MS.gene031200:CDS 35.0%
ATGTTGGAGACAAGATGAAA+CGG - 21207:22215-22234 MS.gene031200:CDS 35.0%
ATTTGGATATGATCACCCAA+TGG - 21207:22306-22325 MS.gene031200:CDS 35.0%
CTTCTTTGCAAGGAATTGTT+AGG + 21207:22336-22355 None:intergenic 35.0%
TTTGGATATGATCACCCAAT+GGG - 21207:22307-22326 MS.gene031200:CDS 35.0%
! ACATTACTGCTAGCTTCAAT+GGG - 21207:22368-22387 MS.gene031200:CDS 35.0%
AATGAAGTTCGTCTAATGGC+AGG + 21207:22136-22155 None:intergenic 40.0%
AGTCAAGCAGAGGAAGAATT+TGG - 21207:22289-22308 MS.gene031200:CDS 40.0%
GAAAGTTGTGAATGTGCCAA+AGG - 21207:22177-22196 MS.gene031200:CDS 40.0%
TCACAACTTTCGATGATGCT+TGG + 21207:22168-22187 None:intergenic 40.0%
TTCGTCTAATGGCAGGTAAA+CGG + 21207:22129-22148 None:intergenic 40.0%
! CACATTACTGCTAGCTTCAA+TGG - 21207:22367-22386 MS.gene031200:CDS 40.0%
!! AAACTTGTTGAGTCAAGCAG+AGG - 21207:22279-22298 MS.gene031200:CDS 40.0%
AGCTTGGTTCAAGTACGACA+TGG + 21207:22251-22270 None:intergenic 45.0%
CTTGGTTCAAGTACGACATG+GGG + 21207:22249-22268 None:intergenic 45.0%
GAATTGTTAGGCCACCCATT+GGG + 21207:22324-22343 None:intergenic 45.0%
GCTTGGTTCAAGTACGACAT+GGG + 21207:22250-22269 None:intergenic 45.0%
GGCTATCTTGCAGTGTATGT+TGG - 21207:22199-22218 MS.gene031200:CDS 45.0%
TACACTGCAAGATAGCCCTT+TGG + 21207:22196-22215 None:intergenic 45.0%
GGAATTGTTAGGCCACCCAT+TGG + 21207:22325-22344 None:intergenic 50.0%
GGATATGATCACCCAATGGG+TGG - 21207:22310-22329 MS.gene031200:CDS 50.0%


Chromosome Type Strat End Strand Name
21207 gene 22118 22396 22118 ID=MS.gene031200
21207 mRNA 22118 22396 22118 ID=MS.gene031200.t1;Parent=MS.gene031200
21207 exon 22118 22396 22118 ID=MS.gene031200.t1.exon1;Parent=MS.gene031200.t1
21207 CDS 22118 22396 22118 ID=cds.MS.gene031200.t1;Parent=MS.gene031200.t1
Gene Sequence

>MS.gene031200

ATGGGTTTCCGTTTACCTGCCATTAGACGAACTTCATTTACAGGTAGCCAAGCATCATCGAAAGTTGTGAATGTGCCAAAGGGCTATCTTGCAGTGTATGTTGGAGACAAGATGAAACGGTTTGTGATCCCCATGTCGTACTTGAACCAAGCTTCATTTCAAAACTTGTTGAGTCAAGCAGAGGAAGAATTTGGATATGATCACCCAATGGGTGGCCTAACAATTCCTTGCAAAGAAGATGTTTTCTTGCACATTACTGCTAGCTTCAATGGGCTATAG
Protein sequence

>MS.gene031200.t1

MGFRLPAIRRTSFTGSQASSKVVNVPKGYLAVYVGDKMKRFVIPMSYLNQASFQNLLSQAEEEFGYDHPMGGLTIPCKEDVFLHITASFNGL