AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene003608


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003608.t1 MTR_4g072490 90.588 85 8 0 1 85 1 85 1.95e-53 160
MS.gene003608.t1 MTR_4g072740 88.235 85 10 0 1 85 1 85 1.35e-51 156
MS.gene003608.t1 MTR_4g072630 86.047 86 11 1 1 85 1 86 6.30e-49 149
MS.gene003608.t1 MTR_4g072465 84.884 86 12 1 1 85 1 86 1.34e-48 149
MS.gene003608.t1 MTR_4g072580 83.529 85 14 0 1 85 1 85 1.82e-48 148
MS.gene003608.t1 MTR_4g072470 82.353 85 15 0 1 85 1 85 4.51e-48 147
MS.gene003608.t1 MTR_4g072830 81.609 87 13 1 1 84 1 87 2.07e-46 143
MS.gene003608.t1 MTR_4g072370 79.545 88 15 1 1 85 1 88 2.29e-46 143
MS.gene003608.t1 MTR_4g072670 86.047 86 11 1 1 85 1 86 8.74e-46 141
MS.gene003608.t1 MTR_4g072600 78.824 85 18 0 1 85 1 85 1.88e-44 138
MS.gene003608.t1 MTR_4g072720 84.884 86 12 1 1 85 1 86 4.54e-44 137
MS.gene003608.t1 MTR_4g072330 72.727 88 21 1 1 85 1 88 5.46e-44 137
MS.gene003608.t1 MTR_4g072540 83.721 86 13 1 1 85 1 86 1.15e-43 136
MS.gene003608.t1 MTR_4g072510 83.721 86 13 1 1 85 1 86 1.87e-43 135
MS.gene003608.t1 MTR_3g084170 72.222 90 20 1 1 85 1 90 1.88e-43 136
MS.gene003608.t1 MTR_4g072610 77.647 85 18 1 1 85 1 84 2.69e-43 135
MS.gene003608.t1 MTR_3g084150 74.725 91 16 2 1 85 1 90 2.75e-43 135
MS.gene003608.t1 MTR_8g461410 73.626 91 17 2 1 85 1 90 8.43e-43 134
MS.gene003608.t1 MTR_3g084180 73.626 91 17 2 1 85 1 90 1.03e-42 134
MS.gene003608.t1 MTR_3g084240 71.111 90 21 1 1 85 1 90 1.84e-42 133
MS.gene003608.t1 MTR_4g072650 77.647 85 18 1 1 85 1 84 1.88e-42 133
MS.gene003608.t1 MTR_3g110032 70.330 91 21 1 1 85 1 91 4.76e-42 132
MS.gene003608.t1 MTR_3g084220 70.000 90 22 1 1 85 1 90 1.19e-41 131
MS.gene003608.t1 MTR_4g072590 81.176 85 16 0 1 85 1 85 1.50e-41 131
MS.gene003608.t1 MTR_3g110038 70.000 90 22 1 1 85 1 90 1.90e-41 130
MS.gene003608.t1 MTR_4g072690 81.176 85 16 0 1 85 1 85 2.04e-41 130
MS.gene003608.t1 MTR_4g050700 69.231 91 22 1 1 85 1 91 6.70e-41 129
MS.gene003608.t1 MTR_2g012410 69.231 91 22 1 1 85 1 91 6.70e-41 129
MS.gene003608.t1 MTR_5g008350 82.432 74 13 0 1 74 1 74 7.94e-41 129
MS.gene003608.t1 MTR_4g072880 71.429 91 20 1 1 85 1 91 8.53e-41 129
MS.gene003608.t1 MTR_4g072450 81.176 85 16 0 1 85 1 85 9.36e-41 129
MS.gene003608.t1 MTR_4g072380 74.419 86 21 1 1 85 1 86 4.93e-40 127
MS.gene003608.t1 MTR_4g072310 68.132 91 23 1 1 85 1 91 5.88e-40 127
MS.gene003608.t1 MTR_3g110042 73.333 90 17 2 1 84 1 89 6.81e-40 127
MS.gene003608.t1 MTR_4g072250 71.429 91 19 2 1 85 1 90 2.13e-39 125
MS.gene003608.t1 MTR_4g072870 67.033 91 24 1 1 85 1 91 2.55e-39 125
MS.gene003608.t1 MTR_4g072820 74.419 86 20 2 1 85 1 85 3.06e-39 125
MS.gene003608.t1 MTR_3g084260 68.132 91 23 1 1 85 1 91 4.67e-39 125
MS.gene003608.t1 MTR_4g072850 73.913 92 17 1 1 85 1 92 6.26e-39 124
MS.gene003608.t1 MTR_4g072240 65.934 91 25 1 1 85 1 91 3.74e-38 122
MS.gene003608.t1 MTR_4g072480 66.667 90 25 1 1 85 1 90 6.74e-38 122
MS.gene003608.t1 MTR_4g072860 68.132 91 23 1 1 85 1 91 9.13e-38 121
MS.gene003608.t1 MTR_3g117630 69.318 88 21 1 4 85 3 90 1.77e-37 120
MS.gene003608.t1 MTR_4g072620 64.444 90 27 1 1 85 1 90 2.74e-37 120
MS.gene003608.t1 MTR_4g072270 65.934 91 25 1 1 85 1 91 4.29e-37 120
MS.gene003608.t1 MTR_4g072570 66.667 90 25 1 1 85 1 90 4.96e-37 119
MS.gene003608.t1 MTR_4g072890 64.835 91 26 1 1 85 1 91 7.72e-37 119
MS.gene003608.t1 MTR_4g072220 64.835 91 26 1 1 85 1 91 9.65e-37 119
MS.gene003608.t1 MTR_3g084250 66.667 90 25 1 1 85 1 90 1.15e-36 119
MS.gene003608.t1 MTR_4g072300 63.736 91 27 1 1 85 1 91 2.10e-36 118
MS.gene003608.t1 MTR_4g072230 67.033 91 22 2 1 85 1 89 3.14e-36 117
MS.gene003608.t1 MTR_4g072660 63.529 85 31 0 1 85 1 85 4.02e-36 117
MS.gene003608.t1 MTR_4g072680 64.444 90 27 1 1 85 1 90 4.55e-36 117
MS.gene003608.t1 MTR_4g072260 63.736 91 27 1 1 85 1 91 5.34e-36 117
MS.gene003608.t1 MTR_4g072500 63.333 90 28 1 1 85 1 90 7.53e-36 117
MS.gene003608.t1 MTR_4g072730 62.222 90 29 1 1 85 1 90 1.30e-35 116
MS.gene003608.t1 MTR_4g072640 63.333 90 28 1 1 85 1 90 3.78e-35 115
MS.gene003608.t1 MTR_4g072530 62.222 90 29 1 1 85 1 90 3.93e-35 115
MS.gene003608.t1 MTR_4g072460 62.222 90 29 1 1 85 1 90 7.87e-35 114
MS.gene003608.t1 MTR_3g084210 63.333 90 28 1 1 85 1 90 1.32e-34 114
MS.gene003608.t1 MTR_3g084190 65.556 90 26 1 1 85 1 90 2.63e-34 113
MS.gene003608.t1 MTR_3g084160 64.444 90 27 1 1 85 1 90 4.08e-34 112
MS.gene003608.t1 MTR_8g461420 64.444 90 27 1 1 85 1 90 4.91e-34 112
MS.gene003608.t1 MTR_5g008360 65.385 78 27 0 8 85 4 81 9.02e-34 111
MS.gene003608.t1 MTR_3g084230 63.333 90 28 1 1 85 1 90 1.44e-33 111
MS.gene003608.t1 MTR_4g072940 70.000 90 20 1 3 85 33 122 1.57e-33 112
MS.gene003608.t1 MTR_3g110045 63.636 88 27 1 3 85 62 149 3.07e-32 109
MS.gene003608.t1 MTR_5g008370 76.471 68 16 0 18 85 23 90 1.90e-31 107
MS.gene003608.t1 MTR_3g084200 68.056 72 18 1 1 67 1 72 2.09e-30 103
MS.gene003608.t1 MTR_4g072560 50.515 97 35 2 1 84 1 97 2.06e-29 100
MS.gene003608.t1 MTR_4g072980 52.174 92 36 2 1 84 1 92 2.19e-29 100
MS.gene003608.t1 MTR_4g072550 64.789 71 25 0 15 85 31 101 9.96e-28 96.7
MS.gene003608.t1 MTR_4g072770 66.667 66 22 0 20 85 15 80 1.28e-27 95.9
MS.gene003608.t1 MTR_4g072910 59.722 72 29 0 14 85 29 100 1.76e-27 96.3
MS.gene003608.t1 MTR_3g117640 46.000 100 39 2 1 85 1 100 2.71e-27 95.9
MS.gene003608.t1 MTR_4g072290 55.294 85 30 1 1 85 1 77 2.16e-25 90.1
MS.gene003608.t1 MTR_3g110035 62.500 64 24 0 22 85 73 136 1.27e-23 87.4
MS.gene003608.t1 MTR_4g072750 60.274 73 22 1 1 66 1 73 1.26e-22 83.2
MS.gene003608.t1 MTR_4g072280 62.687 67 19 1 1 61 1 67 3.98e-22 82.0
MS.gene003608.t1 MTR_4g072930 47.500 80 37 2 11 85 31 110 2.42e-20 78.2
MS.gene003608.t1 MTR_3g117610 53.846 65 29 1 18 82 43 106 2.93e-20 78.2
MS.gene003608.t1 MTR_4g072190 48.101 79 36 2 8 82 20 97 8.05e-20 76.6
MS.gene003608.t1 MTR_6g077850 49.254 67 34 0 19 85 33 99 1.01e-19 76.3
MS.gene003608.t1 MTR_3g113310 49.254 67 33 1 18 84 40 105 1.07e-19 76.6
MS.gene003608.t1 MTR_8g461400 46.667 90 17 2 1 85 1 64 3.22e-19 74.3
MS.gene003608.t1 MTR_4g072840 49.412 85 18 2 1 85 1 60 2.04e-18 72.0
MS.gene003608.t1 MTR_2g043960 55.932 59 25 1 19 77 75 132 1.01e-16 70.5
MS.gene003608.t1 MTR_5g021820 48.485 66 33 1 19 84 39 103 1.19e-16 68.6
MS.gene003608.t1 MTR_4g124750 54.237 59 26 1 19 77 68 125 1.40e-16 69.7
MS.gene003608.t1 MTR_8g076040 50.000 64 31 1 19 82 40 102 6.89e-16 66.6
MS.gene003608.t1 MTR_1g009150 50.725 69 33 1 12 80 63 130 4.16e-15 66.6
MS.gene003608.t1 MTR_1g094750 51.613 62 29 1 19 80 49 109 7.07e-15 64.3
MS.gene003608.t1 MTR_7g101740 46.875 64 33 1 19 82 39 101 3.36e-14 62.8
MS.gene003608.t1 MTR_1g063950 43.548 62 34 1 19 80 47 107 6.36e-14 62.4
MS.gene003608.t1 MTR_3g105900 63.636 44 16 0 17 60 1169 1212 7.86e-14 65.1
MS.gene003608.t1 MTR_1g061570 51.613 62 29 1 19 80 18 78 8.16e-14 62.0
MS.gene003608.t1 MTR_1g024085 50.847 59 28 1 19 77 36 93 3.00e-13 61.2
MS.gene003608.t1 MTR_4g005320 47.541 61 31 1 20 80 70 129 6.09e-13 60.5
MS.gene003608.t1 MTR_5g008380 84.375 32 5 0 54 85 246 277 7.63e-13 62.0
MS.gene003608.t1 MTR_7g118010 45.588 68 34 2 20 85 23 89 9.35e-13 58.9
MS.gene003608.t1 MTR_4g005310 42.424 66 37 1 16 80 58 123 1.67e-12 59.3
MS.gene003608.t1 MTR_3g092220 36.905 84 50 2 4 85 17 99 3.00e-12 57.8
MS.gene003608.t1 MTR_4g072160 43.662 71 39 1 10 80 27 96 5.43e-12 57.8
MS.gene003608.t1 MTR_3g109160 49.180 61 30 1 20 80 69 128 1.04e-11 57.4
MS.gene003608.t1 MTR_8g026730 41.176 68 37 2 20 85 27 93 1.19e-11 56.2
MS.gene003608.t1 MTR_0388s0020 43.939 66 33 2 11 73 66 130 4.45e-11 55.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003608.t1 AT4G38840 73.529 68 17 1 19 85 32 99 4.62e-32 107
MS.gene003608.t1 AT2G21210 59.211 76 29 1 12 85 20 95 1.31e-28 99.0
MS.gene003608.t1 AT5G18050 58.108 74 30 1 13 85 17 90 3.26e-28 97.8
MS.gene003608.t1 AT4G38850 59.259 81 26 2 8 85 13 89 4.65e-28 97.1
MS.gene003608.t1 AT4G34810 63.291 79 24 2 11 84 39 117 7.98e-28 97.4
MS.gene003608.t1 AT5G18080 57.500 80 31 2 7 85 13 90 8.63e-28 96.7
MS.gene003608.t1 AT2G21210 59.211 76 29 1 12 85 87 162 8.65e-28 99.0
MS.gene003608.t1 AT5G18060 59.459 74 29 1 13 85 17 90 8.82e-28 96.7
MS.gene003608.t1 AT5G18020 55.000 80 33 2 7 85 13 90 3.23e-27 95.1
MS.gene003608.t1 AT2G21200 55.696 79 34 1 7 84 8 86 4.60e-27 94.7
MS.gene003608.t1 AT5G18030 56.250 80 30 2 7 85 13 88 7.97e-27 94.0
MS.gene003608.t1 AT4G38825 58.333 72 29 1 15 85 18 89 1.16e-26 93.6
MS.gene003608.t1 AT4G34800 55.952 84 33 2 1 80 1 84 1.54e-26 93.6
MS.gene003608.t1 AT5G18010 53.750 80 34 2 7 85 13 90 1.44e-25 90.9
MS.gene003608.t1 AT4G13790 49.367 79 38 1 8 84 13 91 1.69e-24 88.2
MS.gene003608.t1 AT3G03840 56.944 72 29 1 16 85 23 94 4.21e-24 87.4
MS.gene003608.t1 AT4G34770 56.000 75 31 1 13 85 28 102 8.07e-24 87.0
MS.gene003608.t1 AT3G03820 53.247 77 33 1 12 85 19 95 1.08e-23 86.3
MS.gene003608.t1 AT3G03850 61.194 67 24 1 21 85 26 92 1.90e-23 85.5
MS.gene003608.t1 AT3G03830 55.263 76 31 1 13 85 17 92 2.41e-23 85.5
MS.gene003608.t1 AT4G34790 62.295 61 21 1 20 78 39 99 1.65e-21 81.3
MS.gene003608.t1 AT4G34780 53.521 71 30 1 17 84 25 95 7.04e-21 79.7
MS.gene003608.t1 AT4G38860 53.846 65 30 0 18 82 38 102 1.47e-20 78.6
MS.gene003608.t1 AT2G21220 50.000 68 33 1 18 85 38 104 4.92e-19 74.7
MS.gene003608.t1 AT1G75580 52.239 67 31 1 18 84 42 107 2.27e-18 73.2
MS.gene003608.t1 AT4G34760 50.769 65 31 1 18 82 41 104 4.60e-18 72.4
MS.gene003608.t1 AT2G16580 50.769 65 31 1 18 82 42 105 8.49e-18 71.6
MS.gene003608.t1 AT4G36110 48.438 64 32 1 19 82 39 101 1.69e-16 68.6
MS.gene003608.t1 AT5G20810 55.932 59 25 1 19 77 78 135 4.02e-16 68.9
MS.gene003608.t1 AT5G20810 55.932 59 25 1 19 77 78 135 6.72e-16 68.9
MS.gene003608.t1 AT2G18010 48.438 64 32 1 19 82 47 109 7.12e-16 67.0
MS.gene003608.t1 AT3G43120 52.542 59 27 1 19 77 78 135 8.49e-16 68.2
MS.gene003608.t1 AT1G19830 52.941 68 30 2 18 84 46 112 1.14e-15 66.6
MS.gene003608.t1 AT5G66260 46.970 66 34 1 20 85 34 98 4.94e-14 62.0
MS.gene003608.t1 AT2G46690 44.444 72 36 2 9 77 13 83 1.33e-13 61.2
MS.gene003608.t1 AT2G37030 47.619 63 32 1 19 81 48 109 1.58e-13 61.2
MS.gene003608.t1 AT3G61900 38.554 83 47 2 6 85 16 97 2.48e-13 61.2
MS.gene003608.t1 AT3G20220 46.154 65 33 2 20 83 47 110 3.70e-13 60.1
MS.gene003608.t1 AT4G34750 39.189 74 44 1 9 82 26 98 4.34e-13 60.8
MS.gene003608.t1 AT4G34750 39.189 74 44 1 9 82 26 98 4.34e-13 60.8
MS.gene003608.t1 AT1G75590 47.458 59 30 1 19 77 41 98 1.19e-12 59.7
MS.gene003608.t1 AT3G51200 41.772 79 40 3 7 80 22 99 1.40e-12 58.5
MS.gene003608.t1 AT1G19840 45.714 70 35 2 10 77 29 97 2.89e-12 58.5
MS.gene003608.t1 AT5G10990 43.478 69 35 2 9 77 33 97 4.21e-12 58.2
MS.gene003608.t1 AT3G53250 44.444 63 31 1 19 81 51 109 9.84e-12 56.6
MS.gene003608.t1 AT3G53250 45.161 62 30 1 19 80 82 139 1.79e-11 56.6
MS.gene003608.t1 AT2G24400 45.205 73 38 2 9 80 52 123 1.94e-11 57.0
MS.gene003608.t1 AT4G31320 42.466 73 40 2 9 80 68 139 2.14e-11 57.0
MS.gene003608.t1 AT4G00880 42.857 77 39 3 3 77 13 86 5.70e-11 54.7
MS.gene003608.t1 AT4G12410 44.118 68 34 2 20 84 76 142 6.70e-11 55.1

Find 21 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCTTGGCTCAACAATTCT+TGG 0.248657 4.1:+30965155 None:intergenic
ACAATTCTTGGAATGAGGTT+TGG 0.392315 4.1:+30965167 None:intergenic
GATGTCCTTCTGATGATAAT+AGG 0.401454 4.1:+30965283 None:intergenic
TTCTGATGATAATAGGTAAA+CGG 0.431960 4.1:+30965290 None:intergenic
GTTTGGTTCAAATATGATAC+AGG 0.443189 4.1:+30965184 None:intergenic
GCTCAACAATTCTTGGAATG+AGG 0.458184 4.1:+30965162 None:intergenic
ATTCAAATTGTTCCTCAGCT+TGG 0.481982 4.1:+30965140 None:intergenic
TACACTGCTAGATATCCCTT+TGG 0.484780 4.1:+30965238 None:intergenic
GACATCATCTTCAAAAGTTG+TGG 0.499899 4.1:-30965266 MS.gene003608:CDS
TTTGAATACGACCCTCCAAT+GGG 0.519750 4.1:-30965124 MS.gene003608:CDS
AAGAAAATTTCTTCTCTGCA+AGG 0.519764 4.1:+30965088 None:intergenic
AAAAGTTGTGGATGTTCCAA+AGG 0.527225 4.1:-30965254 MS.gene003608:CDS
AAAGTTGTGGATGTTCCAAA+GGG 0.543795 4.1:-30965253 MS.gene003608:CDS
ATTTGAATACGACCCTCCAA+TGG 0.545953 4.1:-30965125 MS.gene003608:CDS
GTTTACCTATTATCATCAGA+AGG 0.577855 4.1:-30965288 MS.gene003608:CDS
GGAATAGTGAGACCACCCAT+TGG 0.603922 4.1:+30965109 None:intergenic
ATAGTGAGACCACCCATTGG+AGG 0.607611 4.1:+30965112 None:intergenic
TAGTGAGACCACCCATTGGA+GGG 0.635609 4.1:+30965113 None:intergenic
AGAATTGTTGAGCCAAGCTG+AGG 0.641874 4.1:-30965152 MS.gene003608:CDS
GAATACGACCCTCCAATGGG+TGG 0.665793 4.1:-30965121 MS.gene003608:CDS
ATGTAAGTGAGAAAATGAAG+CGG 0.680120 4.1:-30965216 MS.gene003608:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TTCTGATGATAATAGGTAAA+CGG + chr4.1:30965074-30965093 None:intergenic 25.0%
AAGAAAATTTCTTCTCTGCA+AGG + chr4.1:30965276-30965295 None:intergenic 30.0%
ATGTAAGTGAGAAAATGAAG+CGG - chr4.1:30965145-30965164 MS.gene003608:CDS 30.0%
GTTTACCTATTATCATCAGA+AGG - chr4.1:30965073-30965092 MS.gene003608:CDS 30.0%
! GTTTGGTTCAAATATGATAC+AGG + chr4.1:30965180-30965199 None:intergenic 30.0%
AAAAGTTGTGGATGTTCCAA+AGG - chr4.1:30965107-30965126 MS.gene003608:CDS 35.0%
AAAGTTGTGGATGTTCCAAA+GGG - chr4.1:30965108-30965127 MS.gene003608:CDS 35.0%
ACAATTCTTGGAATGAGGTT+TGG + chr4.1:30965197-30965216 None:intergenic 35.0%
ATTCAAATTGTTCCTCAGCT+TGG + chr4.1:30965224-30965243 None:intergenic 35.0%
GACATCATCTTCAAAAGTTG+TGG - chr4.1:30965095-30965114 MS.gene003608:CDS 35.0%
GATGTCCTTCTGATGATAAT+AGG + chr4.1:30965081-30965100 None:intergenic 35.0%
ATTTGAATACGACCCTCCAA+TGG - chr4.1:30965236-30965255 MS.gene003608:CDS 40.0%
GCTCAACAATTCTTGGAATG+AGG + chr4.1:30965202-30965221 None:intergenic 40.0%
TACACTGCTAGATATCCCTT+TGG + chr4.1:30965126-30965145 None:intergenic 40.0%
TTTGAATACGACCCTCCAAT+GGG - chr4.1:30965237-30965256 MS.gene003608:CDS 40.0%
AGAATTGTTGAGCCAAGCTG+AGG - chr4.1:30965209-30965228 MS.gene003608:CDS 45.0%
CAGCTTGGCTCAACAATTCT+TGG + chr4.1:30965209-30965228 None:intergenic 45.0%
ATAGTGAGACCACCCATTGG+AGG + chr4.1:30965252-30965271 None:intergenic 50.0%
GGAATAGTGAGACCACCCAT+TGG + chr4.1:30965255-30965274 None:intergenic 50.0%
TAGTGAGACCACCCATTGGA+GGG + chr4.1:30965251-30965270 None:intergenic 50.0%
GAATACGACCCTCCAATGGG+TGG - chr4.1:30965240-30965259 MS.gene003608:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 30965063 30965320 30965063 ID=MS.gene003608
chr4.1 mRNA 30965063 30965320 30965063 ID=MS.gene003608.t1;Parent=MS.gene003608
chr4.1 exon 30965063 30965320 30965063 ID=MS.gene003608.t1.exon1;Parent=MS.gene003608.t1
chr4.1 CDS 30965063 30965320 30965063 ID=cds.MS.gene003608.t1;Parent=MS.gene003608.t1
Gene Sequence

>MS.gene003608

ATGGGATTCCGTTTACCTATTATCATCAGAAGGACATCATCTTCAAAAGTTGTGGATGTTCCAAAGGGATATCTAGCAGTGTATGTAAGTGAGAAAATGAAGCGGTTTGTGATTCCTGTATCATATTTGAACCAAACCTCATTCCAAGAATTGTTGAGCCAAGCTGAGGAACAATTTGAATACGACCCTCCAATGGGTGGTCTCACTATTCCTTGCAGAGAAGAAATTTTCTTAGATATCACTTCTCGCTTGAATTGA

Protein sequence

>MS.gene003608.t1

MGFRLPIIIRRTSSSKVVDVPKGYLAVYVSEKMKRFVIPVSYLNQTSFQELLSQAEEQFEYDPPMGGLTIPCREEIFLDITSRLN