AlfalfaGEDB | MS.gene68760

M. sativa cultivar XinJiangDaYe / MS.gene68760

KEGG: K14488
GO: GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
PFAM: PF02519.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68760.t1	GAU34320.1	84.9	93	8	1	1	87	1	93	5.60E-36	159.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68760.t1	P33083	70.3	91	20	2	1	85	1	90	8.1e-28	124.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68760.t1	A0A2Z6ND92	84.9	93	8	1	1	87	1	93	4.0e-36	159.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene057800	MS.gene68760	PPI
MS.gene68760	MS.gene062980	PPI
MS.gene68760	MS.gene23367	PPI
MS.gene057816	MS.gene68760	PPI
MS.gene68760	MS.gene50771	PPI
MS.gene50772	MS.gene68760	PPI
MS.gene68760	MS.gene057802	PPI
MS.gene68760	MS.gene057796	PPI
MS.gene68760	MS.gene50682	PPI
MS.gene057783	MS.gene68760	PPI
MS.gene50688	MS.gene68760	PPI
MS.gene68760	MS.gene50690	PPI
MS.gene50651	MS.gene68760	PPI
MS.gene50662	MS.gene68760	PPI
MS.gene68760	MS.gene50658	PPI
MS.gene50683	MS.gene68760	PPI
MS.gene68760	MS.gene50655	PPI
MS.gene68760	MS.gene28863	PPI
MS.gene68760	MS.gene003613	PPI
MS.gene68760	MS.gene003584	PPI
MS.gene057807	MS.gene68760	PPI
MS.gene68760	MS.gene057791	PPI
MS.gene003607	MS.gene68760	PPI
MS.gene23369	MS.gene68760	PPI
MS.gene003585	MS.gene68760	PPI
MS.gene003581	MS.gene68760	PPI
MS.gene50653	MS.gene68760	PPI
MS.gene003603	MS.gene68760	PPI
MS.gene68760	MS.gene062965	PPI
MS.gene062946	MS.gene68760	PPI
MS.gene057805	MS.gene68760	PPI
MS.gene68760	MS.gene022358	PPI
MS.gene062944	MS.gene68760	PPI
MS.gene68760	MS.gene062953	PPI
MS.gene057798	MS.gene68760	PPI
MS.gene062971	MS.gene68760	PPI
MS.gene68760	MS.gene062986	PPI
MS.gene057780	MS.gene68760	PPI
MS.gene057788	MS.gene68760	PPI
MS.gene50773	MS.gene68760	PPI
MS.gene68760	MS.gene50770	PPI
MS.gene68760	MS.gene062987	PPI
MS.gene062954	MS.gene68760	PPI
MS.gene50657	MS.gene68760	PPI
MS.gene68760	MS.gene057811	PPI
MS.gene68760	MS.gene057817	PPI
MS.gene50685	MS.gene68760	PPI
MS.gene68760	MS.gene70684	PPI
MS.gene68760	MS.gene062964	PPI
MS.gene062962	MS.gene68760	PPI
MS.gene062975	MS.gene68760	PPI
MS.gene50689	MS.gene68760	PPI
MS.gene68760	MS.gene057777	PPI
MS.gene77525	MS.gene68760	PPI
MS.gene68760	MS.gene062945	PPI
MS.gene003576	MS.gene68760	PPI
MS.gene68760	MS.gene057804	PPI
MS.gene062969	MS.gene68760	PPI
MS.gene057782	MS.gene68760	PPI
MS.gene003606	MS.gene68760	PPI
MS.gene68760	MS.gene50656	PPI
MS.gene003626	MS.gene68760	PPI
MS.gene68760	MS.gene50686	PPI
MS.gene68760	MS.gene70683	PPI
MS.gene50673	MS.gene68760	PPI
MS.gene68760	MS.gene50666	PPI
MS.gene062970	MS.gene68760	PPI
MS.gene68760	MS.gene062985	PPI
MS.gene062967	MS.gene68760	PPI
MS.gene062942	MS.gene68760	PPI
MS.gene003605	MS.gene68760	PPI
MS.gene003629	MS.gene68760	PPI
MS.gene68760	MS.gene003622	PPI
MS.gene003597	MS.gene68760	PPI
MS.gene23373	MS.gene68760	PPI
MS.gene68760	MS.gene003599	PPI
MS.gene68760	MS.gene23368	PPI
MS.gene68760	MS.gene003623	PPI
MS.gene68760	MS.gene50668	PPI
MS.gene68760	MS.gene062958	PPI
MS.gene68760	MS.gene003612	PPI
MS.gene68760	MS.gene057794	PPI
MS.gene003582	MS.gene68760	PPI
MS.gene68760	MS.gene062983	PPI
MS.gene50674	MS.gene68760	PPI
MS.gene68760	MS.gene70685	PPI
MS.gene68760	MS.gene062940	PPI
MS.gene68760	MS.gene062989	PPI
MS.gene68760	MS.gene003595	PPI
MS.gene68760	MS.gene062959	PPI
MS.gene057806	MS.gene68760	PPI
MS.gene057809	MS.gene68760	PPI
MS.gene062966	MS.gene68760	PPI
MS.gene68760	MS.gene50664	PPI
MS.gene68760	MS.gene057785	PPI
MS.gene68760	MS.gene50670	PPI
MS.gene057784	MS.gene68760	PPI
MS.gene062978	MS.gene68760	PPI
MS.gene057779	MS.gene68760	PPI
MS.gene68760	MS.gene003598	PPI
MS.gene50669	MS.gene68760	PPI
MS.gene68760	MS.gene062941	PPI
MS.gene003593	MS.gene68760	PPI
MS.gene003579	MS.gene68760	PPI
MS.gene062972	MS.gene68760	PPI
MS.gene68760	MS.gene50660	PPI
MS.gene003610	MS.gene68760	PPI
MS.gene003628	MS.gene68760	PPI
MS.gene003580	MS.gene68760	PPI
MS.gene68760	MS.gene057799	PPI
MS.gene68760	MS.gene003630	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68760.t1	MTR_4g050700	86.022	93	7	1	1	87	1	93	1.02e-53	162
MS.gene68760.t1	MTR_2g012410	86.022	93	7	1	1	87	1	93	1.02e-53	162
MS.gene68760.t1	MTR_3g084170	84.946	93	7	2	1	87	1	92	1.47e-51	156
MS.gene68760.t1	MTR_3g084240	83.871	93	8	2	1	87	1	92	1.65e-50	154
MS.gene68760.t1	MTR_3g110032	81.720	93	11	1	1	87	1	93	1.11e-49	152
MS.gene68760.t1	MTR_3g110038	81.720	93	10	2	1	87	1	92	1.33e-49	151
MS.gene68760.t1	MTR_3g084220	81.720	93	10	2	1	87	1	92	4.84e-49	150
MS.gene68760.t1	MTR_3g084260	81.319	91	11	1	1	85	1	91	2.98e-48	148
MS.gene68760.t1	MTR_8g461410	77.419	93	14	2	1	87	1	92	3.03e-47	145
MS.gene68760.t1	MTR_3g084150	77.419	93	14	2	1	87	1	92	6.83e-47	144
MS.gene68760.t1	MTR_3g084180	76.344	93	15	2	1	87	1	92	5.15e-46	142
MS.gene68760.t1	MTR_3g117630	78.889	90	13	1	4	87	3	92	1.13e-45	141
MS.gene68760.t1	MTR_4g072880	68.817	93	23	1	1	87	1	93	1.04e-42	134
MS.gene68760.t1	MTR_3g110042	76.136	88	14	2	1	82	1	87	1.60e-42	134
MS.gene68760.t1	MTR_4g072310	67.742	93	24	1	1	87	1	93	1.63e-41	131
MS.gene68760.t1	MTR_4g072250	69.892	93	21	2	1	87	1	92	2.60e-41	130
MS.gene68760.t1	MTR_4g072500	69.892	93	21	2	1	87	1	92	9.59e-41	129
MS.gene68760.t1	MTR_4g072270	66.667	93	25	1	1	87	1	93	2.35e-40	128
MS.gene68760.t1	MTR_4g072370	70.330	91	18	2	1	85	1	88	5.41e-40	127
MS.gene68760.t1	MTR_4g072230	69.892	93	20	2	1	87	1	91	9.53e-40	126
MS.gene68760.t1	MTR_4g072530	69.231	91	21	2	1	85	1	90	1.24e-39	126
MS.gene68760.t1	MTR_4g072640	69.565	92	21	2	1	86	1	91	2.09e-39	125
MS.gene68760.t1	MTR_4g072860	66.667	93	25	1	1	87	1	93	6.18e-39	124
MS.gene68760.t1	MTR_4g072330	67.033	91	21	2	1	85	1	88	7.20e-39	124
MS.gene68760.t1	MTR_3g084250	67.742	93	23	2	1	87	1	92	1.46e-38	124
MS.gene68760.t1	MTR_4g072850	71.277	94	20	2	1	87	1	94	4.29e-38	122
MS.gene68760.t1	MTR_4g072220	64.516	93	27	1	1	87	1	93	5.54e-38	122
MS.gene68760.t1	MTR_4g072240	64.835	91	26	1	1	85	1	91	5.77e-38	122
MS.gene68760.t1	MTR_4g072870	63.736	91	27	1	1	85	1	91	9.34e-38	121
MS.gene68760.t1	MTR_4g072470	68.132	91	17	2	1	85	1	85	1.11e-37	121
MS.gene68760.t1	MTR_4g072740	68.132	91	17	2	1	85	1	85	1.71e-37	120
MS.gene68760.t1	MTR_4g072300	63.736	91	27	1	1	85	1	91	4.29e-37	120
MS.gene68760.t1	MTR_4g072260	61.290	93	30	1	1	87	1	93	4.75e-37	120
MS.gene68760.t1	MTR_4g072490	64.835	91	20	2	1	85	1	85	1.01e-36	119
MS.gene68760.t1	MTR_4g072890	62.637	91	28	1	1	85	1	91	1.15e-36	119
MS.gene68760.t1	MTR_4g072480	64.516	93	26	2	1	87	1	92	1.68e-36	118
MS.gene68760.t1	MTR_4g072830	67.778	90	20	2	1	84	1	87	4.45e-36	117
MS.gene68760.t1	MTR_4g072620	63.441	93	27	2	1	87	1	92	8.08e-36	117
MS.gene68760.t1	MTR_4g072580	65.934	91	19	2	1	85	1	85	9.71e-36	116
MS.gene68760.t1	MTR_4g072570	65.591	93	25	2	1	87	1	92	1.10e-35	116
MS.gene68760.t1	MTR_4g072730	63.736	91	26	2	1	85	1	90	3.55e-35	115
MS.gene68760.t1	MTR_3g084200	79.452	73	8	2	1	67	1	72	4.14e-35	115
MS.gene68760.t1	MTR_4g072465	63.736	91	22	2	1	85	1	86	4.48e-35	114
MS.gene68760.t1	MTR_4g072680	62.366	93	28	2	1	87	1	92	8.26e-35	114
MS.gene68760.t1	MTR_3g084190	67.742	93	23	2	1	87	1	92	2.31e-34	113
MS.gene68760.t1	MTR_4g072610	67.033	91	17	3	1	85	1	84	5.00e-34	112
MS.gene68760.t1	MTR_4g072630	62.637	91	23	2	1	85	1	86	7.18e-34	111
MS.gene68760.t1	MTR_8g461420	66.667	93	24	2	1	87	1	92	1.10e-33	111
MS.gene68760.t1	MTR_3g084210	68.817	93	22	2	1	87	1	92	1.82e-33	110
MS.gene68760.t1	MTR_8g461400	70.115	87	5	3	1	87	1	66	2.93e-33	109
MS.gene68760.t1	MTR_4g072940	67.391	92	23	2	3	87	33	124	3.69e-33	111
MS.gene68760.t1	MTR_4g072460	60.215	93	30	2	1	87	1	92	3.75e-33	110
MS.gene68760.t1	MTR_4g072380	63.736	91	22	2	1	85	1	86	4.97e-33	109
MS.gene68760.t1	MTR_4g072820	62.637	91	22	3	1	85	1	85	8.84e-33	108
MS.gene68760.t1	MTR_4g072670	63.736	91	22	2	1	85	1	86	1.07e-32	108
MS.gene68760.t1	MTR_4g072650	64.835	91	19	3	1	85	1	84	1.26e-32	108
MS.gene68760.t1	MTR_3g084230	65.591	93	25	2	1	87	1	92	1.70e-32	108
MS.gene68760.t1	MTR_4g072600	64.773	88	19	2	1	82	1	82	2.21e-32	108
MS.gene68760.t1	MTR_3g084160	64.516	93	26	2	1	87	1	92	3.11e-32	107
MS.gene68760.t1	MTR_4g072720	63.736	91	22	2	1	85	1	86	7.10e-32	106
MS.gene68760.t1	MTR_4g072540	62.637	91	23	2	1	85	1	86	7.84e-32	106
MS.gene68760.t1	MTR_3g110045	68.132	91	22	2	3	87	62	151	9.58e-32	108
MS.gene68760.t1	MTR_4g072660	59.140	93	26	2	1	87	1	87	3.87e-31	105
MS.gene68760.t1	MTR_4g072510	62.637	91	23	2	1	85	1	86	5.95e-31	104
MS.gene68760.t1	MTR_4g072590	59.341	91	25	2	1	85	1	85	2.11e-30	103
MS.gene68760.t1	MTR_4g072450	60.440	91	24	2	1	85	1	85	6.31e-30	102
MS.gene68760.t1	MTR_4g072690	59.341	91	25	2	1	85	1	85	1.05e-29	101
MS.gene68760.t1	MTR_5g008350	63.750	80	17	2	1	74	1	74	4.94e-29	99.4
MS.gene68760.t1	MTR_5g008360	64.474	76	21	1	18	87	8	83	5.77e-29	99.4
MS.gene68760.t1	MTR_5g008370	59.574	94	25	3	1	85	1	90	6.25e-29	100
MS.gene68760.t1	MTR_4g072550	58.696	92	30	2	4	87	12	103	1.28e-28	99.0
MS.gene68760.t1	MTR_4g072980	54.348	92	34	3	1	84	1	92	1.76e-28	98.6
MS.gene68760.t1	MTR_4g072560	51.546	97	34	3	1	84	1	97	6.79e-28	97.1
MS.gene68760.t1	MTR_4g072910	65.217	69	18	1	23	85	32	100	2.76e-27	95.5
MS.gene68760.t1	MTR_4g072770	69.697	66	14	1	26	85	15	80	4.83e-27	94.4
MS.gene68760.t1	MTR_3g117640	50.000	100	35	3	1	85	1	100	5.66e-25	89.7
MS.gene68760.t1	MTR_4g072290	51.724	87	34	1	1	87	1	79	3.00e-24	87.4
MS.gene68760.t1	MTR_4g072280	57.333	75	26	1	1	69	1	75	4.34e-22	82.0
MS.gene68760.t1	MTR_4g072750	62.162	74	19	3	1	66	1	73	2.18e-21	80.1
MS.gene68760.t1	MTR_3g110035	60.606	66	20	1	28	87	73	138	3.76e-21	81.3
MS.gene68760.t1	MTR_4g072840	52.941	85	15	4	1	85	1	60	3.14e-20	76.6
MS.gene68760.t1	MTR_4g072930	50.617	81	29	3	16	85	30	110	1.94e-19	76.3
MS.gene68760.t1	MTR_3g113310	54.688	64	22	2	25	82	41	103	1.17e-18	73.9
MS.gene68760.t1	MTR_3g117610	57.812	64	20	2	25	82	44	106	2.26e-18	73.2
MS.gene68760.t1	MTR_6g077850	48.529	68	29	1	24	85	32	99	1.22e-17	71.2
MS.gene68760.t1	MTR_4g072190	56.250	64	21	2	25	82	35	97	2.07e-17	70.5
MS.gene68760.t1	MTR_5g021820	54.688	64	22	2	25	82	39	101	2.31e-16	68.2
MS.gene68760.t1	MTR_8g076040	54.688	64	22	2	25	82	40	102	3.04e-16	67.8
MS.gene68760.t1	MTR_2g043960	50.704	71	28	2	16	80	66	135	7.90e-16	68.2
MS.gene68760.t1	MTR_4g124750	47.500	80	31	3	13	82	52	130	1.84e-15	67.0
MS.gene68760.t1	MTR_4g005310	50.000	66	26	2	22	80	58	123	2.38e-14	64.3
MS.gene68760.t1	MTR_1g009150	55.556	63	21	2	24	80	69	130	3.46e-14	64.3
MS.gene68760.t1	MTR_1g094750	45.882	85	36	4	5	80	26	109	3.78e-14	62.8
MS.gene68760.t1	MTR_8g461210	41.975	81	40	2	3	77	12	91	2.52e-12	58.2
MS.gene68760.t1	MTR_3g109160	54.098	61	21	2	26	80	69	128	1.36e-11	57.4
MS.gene68760.t1	MTR_4g005320	50.820	61	23	2	26	80	70	129	1.67e-11	57.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68760.t1	AT4G38840	55.556	99	30	3	1	85	1	99	2.34e-30	103
MS.gene68760.t1	AT5G18020	56.579	76	26	2	17	85	15	90	3.85e-26	92.4
MS.gene68760.t1	AT5G18060	56.579	76	26	2	17	85	15	90	1.19e-25	91.3
MS.gene68760.t1	AT5G18050	55.263	76	27	2	17	85	15	90	1.87e-25	90.9
MS.gene68760.t1	AT2G21210	58.228	79	24	3	15	85	18	95	3.00e-25	90.5
MS.gene68760.t1	AT5G18030	61.194	67	19	2	26	85	22	88	7.35e-25	89.4
MS.gene68760.t1	AT5G18080	61.194	67	19	2	26	85	24	90	1.03e-24	89.0
MS.gene68760.t1	AT2G21210	58.228	79	24	3	15	85	85	162	1.66e-24	90.5
MS.gene68760.t1	AT4G34800	50.538	93	32	3	1	84	1	88	2.04e-24	88.2
MS.gene68760.t1	AT3G03840	58.333	72	22	2	22	85	23	94	4.12e-24	87.4
MS.gene68760.t1	AT4G38825	57.971	69	22	2	24	85	21	89	5.30e-24	87.0
MS.gene68760.t1	AT2G21200	60.000	70	21	2	22	84	17	86	8.26e-24	86.7
MS.gene68760.t1	AT5G18010	53.947	76	28	2	17	85	15	90	8.35e-24	86.7
MS.gene68760.t1	AT4G34770	66.667	66	14	2	25	82	34	99	1.21e-23	86.7
MS.gene68760.t1	AT4G38850	56.338	71	22	2	24	85	19	89	1.22e-23	86.3
MS.gene68760.t1	AT3G03850	61.194	67	18	2	27	85	26	92	2.46e-22	82.8
MS.gene68760.t1	AT4G34810	58.824	68	18	2	25	82	47	114	4.04e-22	83.2
MS.gene68760.t1	AT3G03820	53.425	73	25	2	22	85	23	95	4.15e-21	80.1
MS.gene68760.t1	AT3G03830	47.253	91	35	3	8	85	2	92	9.25e-21	79.0
MS.gene68760.t1	AT4G13790	48.193	83	30	3	9	83	13	90	8.31e-20	76.6
MS.gene68760.t1	AT4G34780	55.556	72	23	2	23	85	25	96	2.36e-19	75.9
MS.gene68760.t1	AT4G34790	55.385	65	21	2	26	82	39	103	6.14e-19	74.7
MS.gene68760.t1	AT4G38860	54.688	64	23	1	25	82	39	102	3.15e-18	72.8
MS.gene68760.t1	AT1G75580	59.375	64	19	2	25	82	43	105	5.92e-18	72.4
MS.gene68760.t1	AT4G34760	57.812	64	20	2	25	82	42	104	8.92e-18	71.6
MS.gene68760.t1	AT3G43120	48.611	72	30	2	15	80	68	138	7.56e-17	70.9
MS.gene68760.t1	AT2G21220	52.308	65	24	2	24	82	38	101	2.70e-16	67.8
MS.gene68760.t1	AT4G36110	44.318	88	40	3	5	84	17	103	4.03e-16	67.4
MS.gene68760.t1	AT2G16580	54.688	64	22	2	25	82	43	105	7.42e-16	67.0
MS.gene68760.t1	AT5G20810	54.839	62	21	2	25	80	78	138	2.01e-15	67.4
MS.gene68760.t1	AT5G20810	54.839	62	21	2	25	80	78	138	3.52e-15	67.0
MS.gene68760.t1	AT3G20220	53.030	66	23	3	26	84	47	111	5.65e-15	65.1
MS.gene68760.t1	AT2G18010	51.515	66	25	2	25	84	47	111	1.26e-14	63.9
MS.gene68760.t1	AT1G19830	55.932	59	19	2	25	77	47	104	5.61e-14	62.4
MS.gene68760.t1	AT5G66260	41.304	92	45	3	2	85	8	98	1.68e-12	58.2
MS.gene68760.t1	AT1G75590	41.667	84	38	3	4	77	16	98	1.45e-11	57.0
MS.gene68760.t1	AT2G24400	53.125	64	23	2	23	80	61	123	4.35e-11	56.2

Find 24 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCTTATAGCTGCAGGTAAA+CGG	0.395925	3.2:-92875537	None:intergenic
TTGCTACTTTCAATGACGTT+TGG	0.406264	3.2:-92875579	None:intergenic
AGTCGAGCAGAGGAAGAATT+CGG	0.478004	3.2:+92875685	MS.gene68760:CDS
GTTTACCTGCAGCTATAAGA+AGG	0.480619	3.2:+92875539	MS.gene68760:CDS
CAGCAAGTCTTGAAAGGAAG+TGG	0.481851	3.2:-92875662	None:intergenic
GACGCCCTTCTTATAGCTGC+AGG	0.518710	3.2:-92875544	None:intergenic
TTCGGATACGATCATCCAAT+GGG	0.534809	3.2:+92875703	MS.gene68760:CDS
GGAATTGTGAGGCCACCCAT+TGG	0.542714	3.2:-92875718	None:intergenic
AGCAAGTCTTGAAAGGAAGT+GGG	0.542890	3.2:-92875661	None:intergenic
GGCTATCTTGCGGTATACGT+CGG	0.560024	3.2:+92875613	MS.gene68760:CDS
TCGACTCAGCAAGTCTTGAA+AGG	0.567139	3.2:-92875668	None:intergenic
AAAGTAGCAAATGTGCCAAA+GGG	0.568934	3.2:+92875592	MS.gene68760:CDS
GAAAGTAGCAAATGTGCCAA+AGG	0.572179	3.2:+92875591	MS.gene68760:CDS
AAGAAAACATCTTCTGTGCA+AGG	0.580973	3.2:-92875739	None:intergenic
ATTCGGATACGATCATCCAA+TGG	0.585175	3.2:+92875702	MS.gene68760:CDS
TATACCGCAAGATAGCCCTT+TGG	0.588426	3.2:-92875607	None:intergenic
AGACTTGCTGAGTCGAGCAG+AGG	0.606026	3.2:+92875675	MS.gene68760:CDS
TTTACCTGCAGCTATAAGAA+GGG	0.615768	3.2:+92875540	MS.gene68760:CDS
GCAAGTCTTGAAAGGAAGTG+GGG	0.657898	3.2:-92875660	None:intergenic
TGTGCCAAAGGGCTATCTTG+CGG	0.659086	3.2:+92875603	MS.gene68760:CDS
GGATACGATCATCCAATGGG+TGG	0.680954	3.2:+92875706	MS.gene68760:CDS
TCTTGCGGTATACGTCGGAG+AGG	0.713061	3.2:+92875618	MS.gene68760:CDS
ACGTCGGAGAGGAAATGAAG+CGG	0.723798	3.2:+92875629	MS.gene68760:CDS
CTTCTGTGCAAGGAATTGTG+AGG	0.738315	3.2:-92875729	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAAGTAGCAAATGTGCCAAA+GGG	+	chr3.2:92875592-92875611	MS.gene68760:CDS	35.0%
	AAGAAAACATCTTCTGTGCA+AGG	-	chr3.2:92875742-92875761	None:intergenic	35.0%
	TTCTTATAGCTGCAGGTAAA+CGG	-	chr3.2:92875540-92875559	None:intergenic	35.0%
	TTTACCTGCAGCTATAAGAA+GGG	+	chr3.2:92875540-92875559	MS.gene68760:CDS	35.0%
!!	TTGCTACTTTCAATGACGTT+TGG	-	chr3.2:92875582-92875601	None:intergenic	35.0%
	AGCAAGTCTTGAAAGGAAGT+GGG	-	chr3.2:92875664-92875683	None:intergenic	40.0%
	ATTCGGATACGATCATCCAA+TGG	+	chr3.2:92875702-92875721	MS.gene68760:CDS	40.0%
	GAAAGTAGCAAATGTGCCAA+AGG	+	chr3.2:92875591-92875610	MS.gene68760:CDS	40.0%
	GTTTACCTGCAGCTATAAGA+AGG	+	chr3.2:92875539-92875558	MS.gene68760:CDS	40.0%
	TTCGGATACGATCATCCAAT+GGG	+	chr3.2:92875703-92875722	MS.gene68760:CDS	40.0%
	AGTCGAGCAGAGGAAGAATT+CGG	+	chr3.2:92875685-92875704	MS.gene68760:CDS	45.0%
	CAGCAAGTCTTGAAAGGAAG+TGG	-	chr3.2:92875665-92875684	None:intergenic	45.0%
	CTTCTGTGCAAGGAATTGTG+AGG	-	chr3.2:92875732-92875751	None:intergenic	45.0%
	GCAAGTCTTGAAAGGAAGTG+GGG	-	chr3.2:92875663-92875682	None:intergenic	45.0%
	TATACCGCAAGATAGCCCTT+TGG	-	chr3.2:92875610-92875629	None:intergenic	45.0%
	TCGACTCAGCAAGTCTTGAA+AGG	-	chr3.2:92875671-92875690	None:intergenic	45.0%
	ACGTCGGAGAGGAAATGAAG+CGG	+	chr3.2:92875629-92875648	MS.gene68760:CDS	50.0%
	GGATACGATCATCCAATGGG+TGG	+	chr3.2:92875706-92875725	MS.gene68760:CDS	50.0%
	GGCTATCTTGCGGTATACGT+CGG	+	chr3.2:92875613-92875632	MS.gene68760:CDS	50.0%
	TGTGCCAAAGGGCTATCTTG+CGG	+	chr3.2:92875603-92875622	MS.gene68760:CDS	50.0%
	GACGCCCTTCTTATAGCTGC+AGG	-	chr3.2:92875547-92875566	None:intergenic	55.0%
	GGAATTGTGAGGCCACCCAT+TGG	-	chr3.2:92875721-92875740	None:intergenic	55.0%
	TCTTGCGGTATACGTCGGAG+AGG	+	chr3.2:92875618-92875637	MS.gene68760:CDS	55.0%
!!	AGACTTGCTGAGTCGAGCAG+AGG	+	chr3.2:92875675-92875694	MS.gene68760:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	92875529	92875792	92875529	ID=MS.gene68760
chr3.2	mRNA	92875529	92875792	92875529	ID=MS.gene68760.t1;Parent=MS.gene68760
chr3.2	exon	92875529	92875792	92875529	ID=MS.gene68760.t1.exon1;Parent=MS.gene68760.t1
chr3.2	CDS	92875529	92875792	92875529	ID=cds.MS.gene68760.t1;Parent=MS.gene68760.t1

Gene Sequence	>MS.gene68760 ATGGGATTCCGTTTACCTGCAGCTATAAGAAGGGCGTCATTTAGCAGCAGCCAAACGTCATTGAAAGTAGCAAATGTGCCAAAGGGCTATCTTGCGGTATACGTCGGAGAGGAAATGAAGCGGTTTGTGATCCCCACTTCCTTTCAAGACTTGCTGAGTCGAGCAGAGGAAGAATTCGGATACGATCATCCAATGGGTGGCCTCACAATTCCTTGCACAGAAGATGTTTTCTTTCATATTACTTCTCGCTTCAATGAACTATAA
Protein sequence	>MS.gene68760.t1 MGFRLPAAIRRASFSSSQTSLKVANVPKGYLAVYVGEEMKRFVIPTSFQDLLSRAEEEFGYDHPMGGLTIPCTEDVFFHITSRFNEL