AlfalfaGEDB | MS.gene77524

M. sativa cultivar XinJiangDaYe / MS.gene77524

KEGG: K14488
GO: GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
PFAM: PF02519.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77524.t1	AES71920.1	94.6	92	5	0	1	92	1	92	1.20E-41	178.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77524.t1	P33083	74.7	91	21	2	1	90	1	90	4.9e-31	134.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77524.t1	G7JAQ3	94.6	92	5	0	1	92	1	92	8.8e-42	178.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049758	MS.gene77524	0.809972	1.45E-50	-1.69E-46
MS.gene049915	MS.gene77524	0.801673	8.06E-49	-1.69E-46
MS.gene053836	MS.gene77524	0.826406	2.76E-54	-1.69E-46
MS.gene054473	MS.gene77524	0.811995	5.30E-51	-1.69E-46
MS.gene054542	MS.gene77524	0.81195	5.42E-51	-1.69E-46
MS.gene058845	MS.gene77524	0.803154	3.99E-49	-1.69E-46
MS.gene061049	MS.gene77524	0.804195	2.43E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene50669	MS.gene77524	PPI
MS.gene77524	MS.gene50664	PPI
MS.gene77524	MS.gene057785	PPI
MS.gene77524	MS.gene50670	PPI
MS.gene022358	MS.gene77524	PPI
MS.gene003593	MS.gene77524	PPI
MS.gene003579	MS.gene77524	PPI
MS.gene77524	MS.gene70685	PPI
MS.gene003610	MS.gene77524	PPI
MS.gene003628	MS.gene77524	PPI
MS.gene003599	MS.gene77524	PPI
MS.gene003580	MS.gene77524	PPI
MS.gene77524	MS.gene062941	PPI
MS.gene003585	MS.gene77524	PPI
MS.gene77524	MS.gene50660	PPI
MS.gene23368	MS.gene77524	PPI
MS.gene77524	MS.gene003623	PPI
MS.gene003581	MS.gene77524	PPI
MS.gene057796	MS.gene77524	PPI
MS.gene50653	MS.gene77524	PPI
MS.gene77524	MS.gene057794	PPI
MS.gene057816	MS.gene77524	PPI
MS.gene77524	MS.gene003630	PPI
MS.gene50772	MS.gene77524	PPI
MS.gene057799	MS.gene77524	PPI
MS.gene77524	MS.gene23367	PPI
MS.gene057783	MS.gene77524	PPI
MS.gene50688	MS.gene77524	PPI
MS.gene77524	MS.gene057802	PPI
MS.gene50651	MS.gene77524	PPI
MS.gene77524	MS.gene50682	PPI
MS.gene062962	MS.gene77524	PPI
MS.gene50662	MS.gene77524	PPI
MS.gene50683	MS.gene77524	PPI
MS.gene77524	MS.gene50690	PPI
MS.gene77525	MS.gene77524	PPI
MS.gene057807	MS.gene77524	PPI
MS.gene003607	MS.gene77524	PPI
MS.gene23369	MS.gene77524	PPI
MS.gene77524	MS.gene50655	PPI
MS.gene77524	MS.gene003613	PPI
MS.gene003603	MS.gene77524	PPI
MS.gene062946	MS.gene77524	PPI
MS.gene062967	MS.gene77524	PPI
MS.gene057805	MS.gene77524	PPI
MS.gene50773	MS.gene77524	PPI
MS.gene062944	MS.gene77524	PPI
MS.gene057798	MS.gene77524	PPI
MS.gene77524	MS.gene062965	PPI
MS.gene062971	MS.gene77524	PPI
MS.gene23373	MS.gene77524	PPI
MS.gene77524	MS.gene50771	PPI
MS.gene057780	MS.gene77524	PPI
MS.gene057788	MS.gene77524	PPI
MS.gene057811	MS.gene77524	PPI
MS.gene062989	MS.gene77524	PPI
MS.gene062954	MS.gene77524	PPI
MS.gene062987	MS.gene77524	PPI
MS.gene50657	MS.gene77524	PPI
MS.gene062975	MS.gene77524	PPI
MS.gene77524	MS.gene062986	PPI
MS.gene50689	MS.gene77524	PPI
MS.gene50770	MS.gene77524	PPI
MS.gene003576	MS.gene77524	PPI
MS.gene77524	MS.gene062964	PPI
MS.gene062969	MS.gene77524	PPI
MS.gene057782	MS.gene77524	PPI
MS.gene003606	MS.gene77524	PPI
MS.gene77524	MS.gene50658	PPI
MS.gene77524	MS.gene057777	PPI
MS.gene062940	MS.gene77524	PPI
MS.gene77524	MS.gene28863	PPI
MS.gene77524	MS.gene062945	PPI
MS.gene003626	MS.gene77524	PPI
MS.gene003598	MS.gene77524	PPI
MS.gene77524	MS.gene057804	PPI
MS.gene057791	MS.gene77524	PPI
MS.gene50673	MS.gene77524	PPI
MS.gene062970	MS.gene77524	PPI
MS.gene062942	MS.gene77524	PPI
MS.gene003605	MS.gene77524	PPI
MS.gene77524	MS.gene50656	PPI
MS.gene003629	MS.gene77524	PPI
MS.gene77524	MS.gene50686	PPI
MS.gene77524	MS.gene70683	PPI
MS.gene003597	MS.gene77524	PPI
MS.gene77524	MS.gene50666	PPI
MS.gene77524	MS.gene062985	PPI
MS.gene062980	MS.gene77524	PPI
MS.gene062972	MS.gene77524	PPI
MS.gene77524	MS.gene062953	PPI
MS.gene77524	MS.gene003622	PPI
MS.gene003582	MS.gene77524	PPI
MS.gene003584	MS.gene77524	PPI
MS.gene057800	MS.gene77524	PPI
MS.gene77524	MS.gene057817	PPI
MS.gene50674	MS.gene77524	PPI
MS.gene77524	MS.gene50668	PPI
MS.gene77524	MS.gene70684	PPI
MS.gene77524	MS.gene062958	PPI
MS.gene50685	MS.gene77524	PPI
MS.gene77524	MS.gene003612	PPI
MS.gene77524	MS.gene062983	PPI
MS.gene057806	MS.gene77524	PPI
MS.gene057809	MS.gene77524	PPI
MS.gene062966	MS.gene77524	PPI
MS.gene057784	MS.gene77524	PPI
MS.gene77524	MS.gene003595	PPI
MS.gene062978	MS.gene77524	PPI
MS.gene77524	MS.gene062959	PPI
MS.gene057779	MS.gene77524	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77524.t1	MTR_3g084170	94.565	92	5	0	1	92	1	92	4.71e-62	183
MS.gene77524.t1	MTR_3g084240	93.478	92	6	0	1	92	1	92	4.93e-61	181
MS.gene77524.t1	MTR_3g110038	92.391	92	7	0	1	92	1	92	4.95e-60	178
MS.gene77524.t1	MTR_3g084220	90.217	92	9	0	1	92	1	92	1.54e-58	174
MS.gene77524.t1	MTR_4g050700	84.946	93	13	1	1	92	1	93	7.36e-55	165
MS.gene77524.t1	MTR_2g012410	84.946	93	13	1	1	92	1	93	7.36e-55	165
MS.gene77524.t1	MTR_3g084150	86.022	93	11	2	1	92	1	92	1.13e-54	164
MS.gene77524.t1	MTR_3g084180	84.946	93	12	2	1	92	1	92	9.11e-54	162
MS.gene77524.t1	MTR_8g461410	82.796	93	14	2	1	92	1	92	5.05e-53	160
MS.gene77524.t1	MTR_3g110032	82.796	93	15	1	1	92	1	93	7.10e-52	157
MS.gene77524.t1	MTR_3g084260	81.319	91	16	1	1	90	1	91	9.38e-50	152
MS.gene77524.t1	MTR_3g110042	82.955	88	13	2	1	87	1	87	5.96e-49	150
MS.gene77524.t1	MTR_4g072880	75.269	93	22	1	1	92	1	93	1.41e-48	149
MS.gene77524.t1	MTR_4g072310	76.344	93	21	1	1	92	1	93	2.12e-48	149
MS.gene77524.t1	MTR_3g117630	78.889	90	18	1	4	92	3	92	3.72e-48	148
MS.gene77524.t1	MTR_4g072250	76.344	93	20	2	1	92	1	92	1.79e-47	146
MS.gene77524.t1	MTR_4g072270	74.194	93	23	1	1	92	1	93	5.70e-47	145
MS.gene77524.t1	MTR_3g084200	93.056	72	5	0	1	72	1	72	4.75e-46	143
MS.gene77524.t1	MTR_4g072230	76.344	93	19	2	1	92	1	91	6.13e-46	142
MS.gene77524.t1	MTR_4g072470	75.556	90	17	1	1	90	1	85	9.48e-46	142
MS.gene77524.t1	MTR_4g072740	74.444	90	18	1	1	90	1	85	1.36e-45	141
MS.gene77524.t1	MTR_4g072370	73.333	90	22	1	1	90	1	88	1.65e-45	141
MS.gene77524.t1	MTR_4g072870	71.429	91	25	1	1	90	1	91	3.36e-45	140
MS.gene77524.t1	MTR_4g072490	73.333	90	19	1	1	90	1	85	3.58e-45	140
MS.gene77524.t1	MTR_4g072330	72.222	90	23	1	1	90	1	88	7.11e-45	140
MS.gene77524.t1	MTR_4g072240	72.527	91	24	1	1	90	1	91	7.57e-45	139
MS.gene77524.t1	MTR_4g072260	69.892	93	27	1	1	92	1	93	1.29e-44	139
MS.gene77524.t1	MTR_4g072860	72.043	93	25	1	1	92	1	93	2.20e-44	139
MS.gene77524.t1	MTR_4g072300	72.527	91	24	1	1	90	1	91	2.67e-44	138
MS.gene77524.t1	MTR_4g072220	70.968	93	26	1	1	92	1	93	4.35e-44	138
MS.gene77524.t1	MTR_4g072890	71.429	91	25	1	1	90	1	91	4.52e-44	137
MS.gene77524.t1	MTR_4g072580	71.111	90	21	1	1	90	1	85	1.85e-43	136
MS.gene77524.t1	MTR_4g072850	71.277	94	25	1	1	92	1	94	2.35e-43	136
MS.gene77524.t1	MTR_4g072830	73.034	89	22	1	1	89	1	87	2.51e-43	135
MS.gene77524.t1	MTR_4g072480	70.968	93	25	2	1	92	1	92	2.73e-43	135
MS.gene77524.t1	MTR_4g072680	72.043	93	24	2	1	92	1	92	2.82e-43	135
MS.gene77524.t1	MTR_4g072640	72.826	92	23	2	1	91	1	91	7.99e-43	134
MS.gene77524.t1	MTR_4g072620	69.892	93	26	2	1	92	1	92	9.02e-43	134
MS.gene77524.t1	MTR_4g072500	70.968	93	25	2	1	92	1	92	1.06e-42	134
MS.gene77524.t1	MTR_4g072610	72.222	90	19	2	1	90	1	84	3.11e-42	133
MS.gene77524.t1	MTR_4g072630	70.000	90	23	1	1	90	1	86	3.87e-42	132
MS.gene77524.t1	MTR_4g072465	70.000	90	23	1	1	90	1	86	4.56e-42	132
MS.gene77524.t1	MTR_4g072730	66.667	90	30	0	1	90	1	90	5.22e-42	132
MS.gene77524.t1	MTR_4g072570	70.968	93	25	2	1	92	1	92	5.82e-42	132
MS.gene77524.t1	MTR_3g084250	69.892	93	26	2	1	92	1	92	8.00e-42	132
MS.gene77524.t1	MTR_4g072460	69.892	93	26	2	1	92	1	92	1.24e-41	131
MS.gene77524.t1	MTR_4g072530	70.330	91	25	2	1	90	1	90	1.35e-41	131
MS.gene77524.t1	MTR_4g072650	72.222	90	19	2	1	90	1	84	2.32e-41	130
MS.gene77524.t1	MTR_4g072380	72.527	91	19	2	1	90	1	86	2.88e-40	128
MS.gene77524.t1	MTR_4g072670	70.000	90	23	1	1	90	1	86	4.68e-40	127
MS.gene77524.t1	MTR_4g072540	71.111	90	22	1	1	90	1	86	5.83e-40	127
MS.gene77524.t1	MTR_3g084190	66.304	92	31	0	1	92	1	92	1.01e-39	127
MS.gene77524.t1	MTR_4g072720	70.000	90	23	1	1	90	1	86	2.89e-39	125
MS.gene77524.t1	MTR_4g072510	68.889	90	24	1	1	90	1	86	3.05e-39	125
MS.gene77524.t1	MTR_4g072600	68.966	87	22	1	1	87	1	82	4.00e-39	125
MS.gene77524.t1	MTR_3g084210	65.217	92	32	0	1	92	1	92	5.28e-39	125
MS.gene77524.t1	MTR_4g072590	72.222	90	20	1	1	90	1	85	8.23e-39	124
MS.gene77524.t1	MTR_4g072820	69.231	91	21	3	1	90	1	85	9.35e-39	124
MS.gene77524.t1	MTR_4g072660	64.130	92	28	1	1	92	1	87	1.07e-38	124
MS.gene77524.t1	MTR_3g084160	65.217	92	32	0	1	92	1	92	2.77e-38	123
MS.gene77524.t1	MTR_4g072940	68.478	92	27	1	3	92	33	124	3.40e-38	124
MS.gene77524.t1	MTR_8g461420	63.043	92	34	0	1	92	1	92	3.68e-38	123
MS.gene77524.t1	MTR_3g084230	63.043	92	34	0	1	92	1	92	1.22e-37	121
MS.gene77524.t1	MTR_3g110045	65.556	90	31	0	3	92	62	151	7.62e-37	121
MS.gene77524.t1	MTR_5g008350	69.620	79	19	1	1	79	1	74	2.39e-36	118
MS.gene77524.t1	MTR_5g008370	65.556	90	31	0	1	90	1	90	2.59e-36	120
MS.gene77524.t1	MTR_5g008360	72.152	79	21	1	15	92	5	83	3.94e-36	117
MS.gene77524.t1	MTR_4g072450	70.000	90	22	1	1	90	1	85	6.92e-36	117
MS.gene77524.t1	MTR_4g072690	68.889	90	23	1	1	90	1	85	8.71e-36	117
MS.gene77524.t1	MTR_8g461400	65.217	92	6	1	1	92	1	66	4.39e-35	114
MS.gene77524.t1	MTR_4g072980	59.783	92	34	2	1	89	1	92	7.01e-34	112
MS.gene77524.t1	MTR_4g072550	59.783	92	34	1	4	92	12	103	1.69e-33	111
MS.gene77524.t1	MTR_4g072770	72.727	66	18	0	25	90	15	80	2.68e-31	105
MS.gene77524.t1	MTR_4g072560	52.577	97	38	4	1	89	1	97	2.61e-30	103
MS.gene77524.t1	MTR_4g072910	62.162	74	28	0	17	90	27	100	3.44e-30	103
MS.gene77524.t1	MTR_4g072290	59.140	93	23	2	1	92	1	79	6.65e-30	101
MS.gene77524.t1	MTR_3g117640	50.000	100	40	2	1	90	1	100	1.70e-29	101
MS.gene77524.t1	MTR_4g072280	62.667	75	27	1	1	74	1	75	2.65e-26	92.8
MS.gene77524.t1	MTR_3g110035	65.152	66	23	0	27	92	73	138	2.99e-26	94.4
MS.gene77524.t1	MTR_4g072750	64.865	74	22	2	1	71	1	73	1.42e-25	90.9
MS.gene77524.t1	MTR_4g072930	54.321	81	31	2	16	90	30	110	2.78e-25	91.3
MS.gene77524.t1	MTR_3g113310	58.462	65	26	1	23	87	40	103	6.96e-24	87.4
MS.gene77524.t1	MTR_3g117610	60.000	65	25	1	23	87	43	106	5.21e-23	85.5
MS.gene77524.t1	MTR_6g077850	53.731	67	31	0	24	90	33	99	1.21e-22	84.0
MS.gene77524.t1	MTR_4g072190	49.398	83	40	2	5	87	17	97	2.56e-22	83.2
MS.gene77524.t1	MTR_4g072840	52.273	88	10	4	1	87	1	57	3.67e-20	76.6
MS.gene77524.t1	MTR_5g021820	48.780	82	37	3	10	87	21	101	1.04e-19	76.6
MS.gene77524.t1	MTR_2g043960	54.839	62	27	1	24	85	75	135	4.17e-19	76.6
MS.gene77524.t1	MTR_8g076040	53.125	64	29	1	24	87	40	102	5.87e-19	74.7
MS.gene77524.t1	MTR_4g124750	53.125	64	29	1	24	87	68	130	1.13e-18	75.5
MS.gene77524.t1	MTR_1g009150	56.452	62	26	1	24	85	70	130	1.78e-17	73.2
MS.gene77524.t1	MTR_1g094750	47.059	85	40	2	5	85	26	109	2.12e-17	71.2
MS.gene77524.t1	MTR_4g005310	49.296	71	32	2	16	85	56	123	1.30e-16	70.1
MS.gene77524.t1	MTR_3g109160	50.649	77	36	2	10	85	53	128	7.89e-16	68.6
MS.gene77524.t1	MTR_4g005320	50.820	61	29	1	25	85	70	129	2.65e-15	67.0
MS.gene77524.t1	MTR_1g024085	43.902	82	42	2	4	82	13	93	1.27e-14	64.7
MS.gene77524.t1	MTR_8g461210	41.379	87	41	3	4	82	7	91	2.34e-14	63.5
MS.gene77524.t1	MTR_4g072160	50.000	64	31	1	22	85	34	96	2.98e-14	63.9
MS.gene77524.t1	MTR_3g105900	80.556	36	7	0	24	59	1171	1206	4.34e-14	66.2
MS.gene77524.t1	MTR_0388s0020	42.857	70	36	2	11	77	61	129	5.05e-14	63.5
MS.gene77524.t1	MTR_7g118010	48.438	64	30	2	24	85	22	84	6.02e-14	62.0
MS.gene77524.t1	MTR_3g092220	43.478	69	36	2	16	82	24	91	8.28e-14	62.0
MS.gene77524.t1	MTR_3g098970	33.750	80	52	1	13	92	41	119	2.84e-13	61.6
MS.gene77524.t1	MTR_4g025830	42.647	68	35	2	25	89	79	145	3.19e-13	61.6
MS.gene77524.t1	MTR_3g097120	40.244	82	40	2	18	91	2	82	3.68e-13	60.1
MS.gene77524.t1	MTR_7g405740	47.541	61	31	1	25	85	67	126	4.48e-13	61.2
MS.gene77524.t1	MTR_4g021810	48.101	79	34	4	10	82	6	83	5.73e-13	59.7
MS.gene77524.t1	MTR_7g101740	46.875	64	33	1	24	87	39	101	6.98e-13	59.7
MS.gene77524.t1	MTR_1g061570	48.387	62	31	1	24	85	18	78	8.97e-13	59.7
MS.gene77524.t1	MTR_1g063950	38.750	80	46	2	8	85	29	107	9.68e-13	59.3
MS.gene77524.t1	MTR_8g026730	44.444	63	32	2	25	85	27	88	1.61e-12	58.5
MS.gene77524.t1	MTR_8g101520	37.079	89	50	2	8	91	28	115	2.86e-12	58.9
MS.gene77524.t1	MTR_1g059770	41.270	63	36	1	25	87	49	110	6.99e-12	57.4
MS.gene77524.t1	MTR_2g066830	39.759	83	39	3	2	82	41	114	2.46e-11	56.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77524.t1	AT4G38840	55.556	99	35	2	1	90	1	99	9.44e-36	117
MS.gene77524.t1	AT5G18020	69.737	76	22	1	16	90	15	90	2.19e-35	116
MS.gene77524.t1	AT5G18080	67.105	76	24	1	16	90	15	90	9.03e-34	112
MS.gene77524.t1	AT5G18050	65.789	76	25	1	16	90	15	90	3.05e-33	110
MS.gene77524.t1	AT5G18060	65.789	76	25	1	16	90	15	90	6.86e-33	109
MS.gene77524.t1	AT5G18030	67.105	76	22	2	16	90	15	88	1.92e-32	108
MS.gene77524.t1	AT2G21200	60.000	85	31	2	8	89	2	86	4.30e-32	107
MS.gene77524.t1	AT5G18010	64.474	76	26	1	16	90	15	90	5.81e-32	107
MS.gene77524.t1	AT4G38825	66.667	72	23	1	20	90	18	89	8.11e-32	107
MS.gene77524.t1	AT2G21210	53.846	91	33	2	9	90	5	95	3.27e-30	103
MS.gene77524.t1	AT2G21210	53.846	91	33	2	9	90	72	162	2.54e-29	103
MS.gene77524.t1	AT3G03840	63.889	72	24	1	21	90	23	94	4.02e-29	100
MS.gene77524.t1	AT4G34770	67.606	71	21	1	19	87	29	99	1.15e-28	99.4
MS.gene77524.t1	AT4G38850	59.036	83	22	2	9	88	14	87	2.49e-28	98.2
MS.gene77524.t1	AT3G03850	55.914	93	29	3	8	90	2	92	3.36e-28	98.2
MS.gene77524.t1	AT4G34800	53.261	92	36	3	1	89	1	88	6.81e-28	97.4
MS.gene77524.t1	AT3G03820	54.023	87	37	1	7	90	9	95	2.83e-27	95.9
MS.gene77524.t1	AT4G34810	61.765	68	22	1	24	87	47	114	3.81e-27	96.3
MS.gene77524.t1	AT3G03830	54.255	94	29	3	8	90	2	92	7.40e-27	94.7
MS.gene77524.t1	AT4G13790	52.778	72	32	1	19	88	19	90	3.09e-25	90.5
MS.gene77524.t1	AT4G34780	59.155	71	26	1	23	90	26	96	7.45e-24	87.4
MS.gene77524.t1	AT4G34790	60.000	65	24	1	25	87	39	103	2.53e-23	86.3
MS.gene77524.t1	AT4G38860	58.462	65	27	0	23	87	38	102	2.84e-23	85.9
MS.gene77524.t1	AT1G75580	54.054	74	33	1	14	87	33	105	1.36e-22	84.3
MS.gene77524.t1	AT4G34760	56.923	65	27	1	23	87	41	104	3.03e-21	80.9
MS.gene77524.t1	AT2G21220	53.846	65	29	1	23	87	38	101	1.16e-20	79.3
MS.gene77524.t1	AT4G36110	53.030	66	30	1	24	89	39	103	7.31e-20	77.0
MS.gene77524.t1	AT3G43120	53.226	62	28	1	24	85	78	138	1.78e-19	77.8
MS.gene77524.t1	AT3G20220	50.000	78	37	2	13	89	35	111	2.11e-19	76.3
MS.gene77524.t1	AT2G16580	53.846	65	29	1	23	87	42	105	2.28e-19	76.3
MS.gene77524.t1	AT2G18010	51.515	66	31	1	24	89	47	111	6.74e-19	75.1
MS.gene77524.t1	AT5G20810	54.839	62	27	1	24	85	78	138	1.30e-18	75.5
MS.gene77524.t1	AT1G19830	57.143	63	26	1	20	82	43	104	1.85e-18	73.9
MS.gene77524.t1	AT5G20810	54.839	62	27	1	24	85	78	138	2.78e-18	75.5
MS.gene77524.t1	AT5G66260	44.565	92	47	2	2	90	8	98	3.77e-17	70.1
MS.gene77524.t1	AT5G10990	47.222	72	37	1	11	82	27	97	4.07e-16	68.9
MS.gene77524.t1	AT1G75590	44.048	84	41	2	4	82	16	98	5.61e-16	68.6
MS.gene77524.t1	AT1G19840	45.783	83	40	2	4	82	16	97	4.37e-15	66.2
MS.gene77524.t1	AT4G31320	52.459	61	28	1	25	85	80	139	1.25e-14	65.9
MS.gene77524.t1	AT3G51200	49.254	67	32	2	18	83	32	97	1.38e-14	63.9
MS.gene77524.t1	AT2G24400	51.429	70	30	2	25	91	64	132	2.07e-14	65.1
MS.gene77524.t1	AT4G34750	37.179	78	48	1	10	87	22	98	4.65e-14	63.5
MS.gene77524.t1	AT4G34750	37.179	78	48	1	10	87	22	98	4.65e-14	63.5
MS.gene77524.t1	AT3G12830	41.333	75	43	1	11	85	36	109	7.63e-14	62.8
MS.gene77524.t1	AT2G46690	39.189	74	41	2	17	87	16	88	2.07e-13	61.2
MS.gene77524.t1	AT2G37030	46.032	63	33	1	24	86	48	109	3.37e-13	60.8
MS.gene77524.t1	AT3G61900	42.857	70	36	2	24	90	29	97	4.55e-13	60.8
MS.gene77524.t1	AT3G03847	43.529	85	44	4	3	85	48	130	4.87e-13	60.5
MS.gene77524.t1	AT1G56150	47.541	61	31	1	25	85	38	97	4.42e-12	57.4
MS.gene77524.t1	AT5G53590	39.726	73	38	2	24	91	45	116	4.87e-12	58.2
MS.gene77524.t1	AT4G00880	48.438	64	30	2	24	85	27	89	7.20e-12	57.4
MS.gene77524.t1	AT3G60690	40.580	69	37	2	25	90	88	155	9.75e-12	57.8
MS.gene77524.t1	AT4G09530	41.429	70	35	3	16	82	22	88	1.37e-11	56.2
MS.gene77524.t1	AT1G16510	45.763	59	31	1	24	82	53	110	5.14e-11	55.5

Find 23 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTCTAATAGCTGCAGGTAAA+CGG	0.411664	3.2:+72561525	None:intergenic
AGTCAATCCGAGGAAGAATT+CGG	0.461023	3.2:-72561362	MS.gene77524:CDS
GTTTACCTGCAGCTATTAGA+CGG	0.499927	3.2:-72561523	MS.gene77524:CDS
TTTACCTGCAGCTATTAGAC+GGG	0.518841	3.2:-72561522	MS.gene77524:CDS
GGAATTGTGAGGCCGCCCAT+TGG	0.522416	3.2:+72561329	None:intergenic
GTCATATCCGAATTCTTCCT+CGG	0.528340	3.2:+72561355	None:intergenic
TTCGGATATGACCATCCAAT+GGG	0.541939	3.2:-72561344	MS.gene77524:CDS
TTGTGAGGCCGCCCATTGGA+TGG	0.552821	3.2:+72561333	None:intergenic
GGCTATCTTGCAGTGTACGT+TGG	0.552838	3.2:-72561452	MS.gene77524:CDS
TACACTGCAAGATAGCCCTT+TGG	0.556003	3.2:+72561458	None:intergenic
AAAGCATTGAATGTGCCAAA+GGG	0.566846	3.2:-72561473	MS.gene77524:CDS
AAAAGCATTGAATGTGCCAA+AGG	0.573182	3.2:-72561474	MS.gene77524:CDS
AAGAAAACATCTTCTGTGCA+AGG	0.580973	3.2:+72561308	None:intergenic
ATTCGGATATGACCATCCAA+TGG	0.589403	3.2:-72561345	MS.gene77524:CDS
GACGGGCTTCATTCAGCAGT+AGG	0.592271	3.2:-72561505	MS.gene77524:CDS
GAAGCCCGTCTAATAGCTGC+AGG	0.598914	3.2:+72561518	None:intergenic
AAACTTGTTGAGTCAATCCG+AGG	0.620139	3.2:-72561372	MS.gene77524:CDS
GCTTGGTTCAAGTATGATGT+GGG	0.627509	3.2:+72561404	None:intergenic
GGATATGACCATCCAATGGG+CGG	0.678665	3.2:-72561341	MS.gene77524:CDS
AGCTTGGTTCAAGTATGATG+TGG	0.683400	3.2:+72561403	None:intergenic
CTTGGTTCAAGTATGATGTG+GGG	0.689184	3.2:+72561405	None:intergenic
ACGTTGGAGAGCAAATGAAG+CGG	0.725765	3.2:-72561436	MS.gene77524:CDS
CTTCTGTGCAAGGAATTGTG+AGG	0.738315	3.2:+72561318	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ACAAGTTTTGAAATGAAGCT+TGG	+	chr3.2:72561426-72561445	None:intergenic	30.0%
	AAGAAAACATCTTCTGTGCA+AGG	+	chr3.2:72561505-72561524	None:intergenic	35.0%
!!	AAAAGCATTGAATGTGCCAA+AGG	-	chr3.2:72561336-72561355	MS.gene77524:CDS	35.0%
!!	AAAGCATTGAATGTGCCAAA+GGG	-	chr3.2:72561337-72561356	MS.gene77524:CDS	35.0%
	AGCTTGGTTCAAGTATGATG+TGG	+	chr3.2:72561410-72561429	None:intergenic	40.0%
	AGTCAATCCGAGGAAGAATT+CGG	-	chr3.2:72561448-72561467	MS.gene77524:CDS	40.0%
	ATTCGGATATGACCATCCAA+TGG	-	chr3.2:72561465-72561484	MS.gene77524:CDS	40.0%
	CTTGGTTCAAGTATGATGTG+GGG	+	chr3.2:72561408-72561427	None:intergenic	40.0%
	GCTTGGTTCAAGTATGATGT+GGG	+	chr3.2:72561409-72561428	None:intergenic	40.0%
	GTCATATCCGAATTCTTCCT+CGG	+	chr3.2:72561458-72561477	None:intergenic	40.0%
	GTCTAATAGCTGCAGGTAAA+CGG	+	chr3.2:72561288-72561307	None:intergenic	40.0%
	GTTTACCTGCAGCTATTAGA+CGG	-	chr3.2:72561287-72561306	MS.gene77524:CDS	40.0%
	TTCGGATATGACCATCCAAT+GGG	-	chr3.2:72561466-72561485	MS.gene77524:CDS	40.0%
	TTTACCTGCAGCTATTAGAC+GGG	-	chr3.2:72561288-72561307	MS.gene77524:CDS	40.0%
!!	AAACTTGTTGAGTCAATCCG+AGG	-	chr3.2:72561438-72561457	MS.gene77524:CDS	40.0%
	ACGTTGGAGAGCAAATGAAG+CGG	-	chr3.2:72561374-72561393	MS.gene77524:CDS	45.0%
	CTTCTGTGCAAGGAATTGTG+AGG	+	chr3.2:72561495-72561514	None:intergenic	45.0%
	TACACTGCAAGATAGCCCTT+TGG	+	chr3.2:72561355-72561374	None:intergenic	45.0%
	GGATATGACCATCCAATGGG+CGG	-	chr3.2:72561469-72561488	MS.gene77524:CDS	50.0%
	GGCTATCTTGCAGTGTACGT+TGG	-	chr3.2:72561358-72561377	MS.gene77524:CDS	50.0%
	GAAGCCCGTCTAATAGCTGC+AGG	+	chr3.2:72561295-72561314	None:intergenic	55.0%
!	GACGGGCTTCATTCAGCAGT+AGG	-	chr3.2:72561305-72561324	MS.gene77524:CDS	55.0%
	GGAATTGTGAGGCCGCCCAT+TGG	+	chr3.2:72561484-72561503	None:intergenic	60.0%
	TTGTGAGGCCGCCCATTGGA+TGG	+	chr3.2:72561480-72561499	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	72561277	72561555	72561277	ID=MS.gene77524
chr3.2	mRNA	72561277	72561555	72561277	ID=MS.gene77524.t1;Parent=MS.gene77524
chr3.2	exon	72561277	72561555	72561277	ID=MS.gene77524.t1.exon1;Parent=MS.gene77524.t1
chr3.2	CDS	72561277	72561555	72561277	ID=cds.MS.gene77524.t1;Parent=MS.gene77524.t1

Gene Sequence	>MS.gene77524 ATGGGTTTCCGTTTACCTGCAGCTATTAGACGGGCTTCATTCAGCAGTAGGCAAACATCAAAAGCATTGAATGTGCCAAAGGGCTATCTTGCAGTGTACGTTGGAGAGCAAATGAAGCGGTTTGTTATCCCCACATCATACTTGAACCAAGCTTCATTTCAAAACTTGTTGAGTCAATCCGAGGAAGAATTCGGATATGACCATCCAATGGGCGGCCTCACAATTCCTTGCACAGAAGATGTTTTCTTGCATATTACATCTCGCTTCAATGAGCTATAA
Protein sequence	>MS.gene77524.t1 MGFRLPAAIRRASFSSRQTSKALNVPKGYLAVYVGEQMKRFVIPTSYLNQASFQNLLSQSEEEFGYDHPMGGLTIPCTEDVFLHITSRFNEL