AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene057790


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057790.t1 MTR_4g072740 93.023 86 5 1 1 86 1 85 1.74e-54 163
MS.gene057790.t1 MTR_4g072490 87.209 86 10 1 1 86 1 85 5.85e-52 157
MS.gene057790.t1 MTR_4g072630 83.721 86 14 0 1 86 1 86 2.46e-51 155
MS.gene057790.t1 MTR_4g072465 81.395 86 16 0 1 86 1 86 3.39e-50 152
MS.gene057790.t1 MTR_4g072470 83.721 86 13 1 1 86 1 85 7.12e-49 149
MS.gene057790.t1 MTR_4g072580 80.233 86 16 1 1 86 1 85 5.17e-47 145
MS.gene057790.t1 MTR_4g072370 79.545 88 16 1 1 86 1 88 5.35e-47 145
MS.gene057790.t1 MTR_4g072670 88.372 86 10 0 1 86 1 86 1.17e-46 144
MS.gene057790.t1 MTR_4g072830 80.460 87 15 1 1 85 1 87 3.82e-46 142
MS.gene057790.t1 MTR_3g084150 75.556 90 18 1 1 86 1 90 6.36e-46 142
MS.gene057790.t1 MTR_4g072330 76.404 89 17 2 1 86 1 88 2.41e-45 140
MS.gene057790.t1 MTR_4g072720 87.209 86 11 0 1 86 1 86 3.59e-45 140
MS.gene057790.t1 MTR_3g084180 74.444 90 19 1 1 86 1 90 3.89e-45 140
MS.gene057790.t1 MTR_4g072540 86.047 86 12 0 1 86 1 86 4.05e-45 140
MS.gene057790.t1 MTR_4g072510 86.047 86 12 0 1 86 1 86 6.56e-45 139
MS.gene057790.t1 MTR_4g072600 77.907 86 18 1 1 86 1 85 8.06e-45 139
MS.gene057790.t1 MTR_8g461410 73.333 90 20 1 1 86 1 90 9.25e-45 139
MS.gene057790.t1 MTR_3g084170 73.333 90 20 1 1 86 1 90 2.62e-44 138
MS.gene057790.t1 MTR_4g072610 76.744 86 18 1 1 86 1 84 2.93e-43 135
MS.gene057790.t1 MTR_3g084240 72.222 90 21 1 1 86 1 90 3.65e-43 135
MS.gene057790.t1 MTR_3g110038 71.111 90 22 1 1 86 1 90 4.97e-43 135
MS.gene057790.t1 MTR_4g072650 75.581 86 19 1 1 86 1 84 1.85e-42 133
MS.gene057790.t1 MTR_3g084220 70.000 90 23 1 1 86 1 90 6.33e-42 132
MS.gene057790.t1 MTR_4g050700 70.330 91 22 1 1 86 1 91 7.95e-42 132
MS.gene057790.t1 MTR_2g012410 70.330 91 22 1 1 86 1 91 7.95e-42 132
MS.gene057790.t1 MTR_3g110032 69.231 91 23 1 1 86 1 91 1.27e-41 131
MS.gene057790.t1 MTR_4g072250 73.626 91 18 2 1 86 1 90 3.51e-41 130
MS.gene057790.t1 MTR_4g072310 69.231 91 23 1 1 86 1 91 4.75e-41 130
MS.gene057790.t1 MTR_4g072870 70.652 92 20 2 1 86 1 91 5.64e-41 129
MS.gene057790.t1 MTR_4g072590 80.233 86 16 1 1 86 1 85 6.36e-41 129
MS.gene057790.t1 MTR_4g072880 69.231 91 23 1 1 86 1 91 9.70e-41 129
MS.gene057790.t1 MTR_4g072570 68.889 90 24 1 1 86 1 90 1.76e-40 128
MS.gene057790.t1 MTR_3g084260 68.132 91 24 1 1 86 1 91 2.00e-40 128
MS.gene057790.t1 MTR_4g072480 68.889 90 24 1 1 86 1 90 2.34e-40 128
MS.gene057790.t1 MTR_4g072380 75.862 87 19 2 1 86 1 86 5.25e-40 127
MS.gene057790.t1 MTR_5g008350 80.000 75 14 1 1 75 1 74 6.37e-40 126
MS.gene057790.t1 MTR_4g072850 70.652 92 21 1 1 86 1 92 7.68e-40 127
MS.gene057790.t1 MTR_3g084250 70.000 90 23 1 1 86 1 90 9.85e-40 126
MS.gene057790.t1 MTR_4g072300 67.033 91 25 1 1 86 1 91 1.30e-39 126
MS.gene057790.t1 MTR_4g072820 73.563 87 20 2 1 86 1 85 1.56e-39 126
MS.gene057790.t1 MTR_3g110042 69.663 89 23 1 1 85 1 89 1.92e-39 126
MS.gene057790.t1 MTR_4g072240 65.934 91 26 1 1 86 1 91 2.74e-39 125
MS.gene057790.t1 MTR_4g072690 77.907 86 18 1 1 86 1 85 3.41e-39 125
MS.gene057790.t1 MTR_4g072620 65.556 90 27 1 1 86 1 90 5.76e-39 124
MS.gene057790.t1 MTR_4g072450 77.907 86 18 1 1 86 1 85 1.09e-38 124
MS.gene057790.t1 MTR_4g072860 70.652 92 20 2 1 86 1 91 1.10e-38 124
MS.gene057790.t1 MTR_4g072680 66.667 90 26 1 1 86 1 90 1.16e-38 124
MS.gene057790.t1 MTR_4g072270 67.033 91 25 1 1 86 1 91 1.22e-38 124
MS.gene057790.t1 MTR_4g072220 64.835 91 27 1 1 86 1 91 5.87e-38 122
MS.gene057790.t1 MTR_4g072890 64.835 91 27 1 1 86 1 91 6.82e-38 122
MS.gene057790.t1 MTR_4g072260 63.736 91 28 1 1 86 1 91 9.51e-38 121
MS.gene057790.t1 MTR_3g117630 76.712 73 17 0 14 86 18 90 9.65e-38 121
MS.gene057790.t1 MTR_4g072660 66.279 86 28 1 1 86 1 85 1.03e-37 121
MS.gene057790.t1 MTR_4g072730 64.444 90 28 1 1 86 1 90 1.08e-37 121
MS.gene057790.t1 MTR_4g072460 64.444 90 28 1 1 86 1 90 2.48e-37 120
MS.gene057790.t1 MTR_4g072230 68.132 91 22 2 1 86 1 89 3.20e-37 120
MS.gene057790.t1 MTR_4g072500 65.556 90 27 1 1 86 1 90 3.60e-37 120
MS.gene057790.t1 MTR_3g084210 65.556 90 27 1 1 86 1 90 1.63e-36 118
MS.gene057790.t1 MTR_3g084190 67.778 90 25 1 1 86 1 90 2.77e-36 118
MS.gene057790.t1 MTR_4g072640 64.835 91 26 2 1 86 1 90 3.80e-36 117
MS.gene057790.t1 MTR_8g461420 66.667 90 26 1 1 86 1 90 5.06e-36 117
MS.gene057790.t1 MTR_4g072530 63.333 90 29 1 1 86 1 90 6.63e-36 117
MS.gene057790.t1 MTR_3g084230 65.556 90 27 1 1 86 1 90 2.01e-35 115
MS.gene057790.t1 MTR_3g084160 66.667 90 26 1 1 86 1 90 6.23e-35 114
MS.gene057790.t1 MTR_4g072940 66.667 90 24 1 3 86 33 122 9.08e-35 115
MS.gene057790.t1 MTR_5g008370 63.333 90 29 1 1 86 1 90 3.46e-34 114
MS.gene057790.t1 MTR_3g110045 67.045 88 25 1 3 86 62 149 4.48e-34 114
MS.gene057790.t1 MTR_5g008360 66.667 78 26 0 9 86 4 81 8.51e-34 111
MS.gene057790.t1 MTR_3g084200 69.444 72 18 1 1 68 1 72 1.89e-31 106
MS.gene057790.t1 MTR_4g072770 59.302 86 29 1 1 86 1 80 8.69e-30 101
MS.gene057790.t1 MTR_4g072980 53.261 92 36 2 1 85 1 92 9.18e-30 101
MS.gene057790.t1 MTR_4g072560 49.485 97 37 3 1 85 1 97 1.65e-29 101
MS.gene057790.t1 MTR_4g072550 64.789 71 25 0 16 86 31 101 3.37e-29 100
MS.gene057790.t1 MTR_4g072910 59.740 77 30 1 10 86 25 100 8.14e-28 97.1
MS.gene057790.t1 MTR_3g117640 47.000 100 39 2 1 86 1 100 3.92e-27 95.5
MS.gene057790.t1 MTR_3g110035 65.625 64 22 0 23 86 73 136 1.82e-25 92.0
MS.gene057790.t1 MTR_4g072290 55.814 86 29 2 1 86 1 77 1.30e-24 88.2
MS.gene057790.t1 MTR_4g072280 57.333 75 27 1 1 70 1 75 7.27e-24 86.3
MS.gene057790.t1 MTR_4g072750 61.644 73 22 2 1 67 1 73 1.61e-23 85.5
MS.gene057790.t1 MTR_6g077850 53.731 67 31 0 20 86 33 99 3.26e-22 82.8
MS.gene057790.t1 MTR_3g113310 52.239 67 31 1 19 85 40 105 5.93e-21 79.7
MS.gene057790.t1 MTR_3g117610 55.385 65 28 1 19 83 43 106 9.69e-21 79.3
MS.gene057790.t1 MTR_4g072190 48.000 75 38 1 11 85 26 99 3.20e-20 77.8
MS.gene057790.t1 MTR_4g072930 48.148 81 37 2 11 86 30 110 3.26e-20 78.2
MS.gene057790.t1 MTR_4g072840 51.724 87 14 3 1 86 1 60 6.80e-20 75.9
MS.gene057790.t1 MTR_8g461400 46.667 90 18 2 1 86 1 64 1.32e-19 75.1
MS.gene057790.t1 MTR_2g043960 54.237 59 26 1 20 78 75 132 2.91e-17 72.0
MS.gene057790.t1 MTR_5g021820 48.485 66 33 1 20 85 39 103 5.52e-17 69.7
MS.gene057790.t1 MTR_8g076040 50.000 64 31 1 20 83 40 102 1.48e-16 68.6
MS.gene057790.t1 MTR_4g124750 52.542 59 27 1 20 78 68 125 1.76e-16 69.7
MS.gene057790.t1 MTR_1g094750 53.226 62 28 1 20 81 49 109 9.04e-16 67.0
MS.gene057790.t1 MTR_1g009150 50.000 68 33 1 14 81 64 130 3.68e-15 66.6
MS.gene057790.t1 MTR_3g105900 69.048 42 13 0 20 61 1171 1212 4.84e-15 68.6
MS.gene057790.t1 MTR_1g061570 45.570 79 40 2 5 81 1 78 9.03e-15 64.3
MS.gene057790.t1 MTR_7g101740 52.308 65 28 2 20 83 39 101 1.04e-14 63.9
MS.gene057790.t1 MTR_4g005310 45.455 66 35 1 17 81 58 123 1.07e-14 65.1
MS.gene057790.t1 MTR_1g063950 47.826 69 33 2 14 81 41 107 1.82e-14 63.5
MS.gene057790.t1 MTR_7g118010 47.059 68 33 2 21 86 23 89 8.91e-14 61.6
MS.gene057790.t1 MTR_1g024085 46.575 73 36 2 10 80 24 95 1.16e-13 62.0
MS.gene057790.t1 MTR_3g092220 40.260 77 43 2 12 86 24 99 1.21e-13 61.2
MS.gene057790.t1 MTR_4g005320 47.541 61 31 1 21 81 70 129 2.11e-13 62.0
MS.gene057790.t1 MTR_4g072160 51.613 62 29 1 20 81 36 96 4.67e-13 60.5
MS.gene057790.t1 MTR_3g109160 52.459 61 28 1 21 81 69 128 5.23e-13 60.8
MS.gene057790.t1 MTR_8g461210 47.059 68 32 2 20 84 34 100 2.71e-12 57.8
MS.gene057790.t1 MTR_8g026730 41.176 68 37 2 21 86 27 93 1.38e-11 55.8
MS.gene057790.t1 MTR_7g405740 40.260 77 44 2 5 81 52 126 2.04e-11 56.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057790.t1 AT4G38840 54.545 99 32 2 1 86 1 99 3.91e-32 107
MS.gene057790.t1 AT5G18080 60.759 79 29 2 9 86 13 90 1.03e-29 101
MS.gene057790.t1 AT4G34800 57.317 82 32 1 1 79 1 82 1.04e-29 101
MS.gene057790.t1 AT2G21210 54.321 81 35 1 8 86 15 95 2.41e-29 100
MS.gene057790.t1 AT5G18060 59.494 79 30 2 9 86 13 90 2.55e-29 100
MS.gene057790.t1 AT5G18050 58.228 79 31 2 9 86 13 90 2.94e-29 100
MS.gene057790.t1 AT5G18020 58.228 79 31 2 9 86 13 90 3.49e-29 100
MS.gene057790.t1 AT4G38825 57.500 80 30 2 8 86 13 89 7.18e-29 99.4
MS.gene057790.t1 AT2G21210 54.321 81 35 1 8 86 82 162 1.22e-28 100
MS.gene057790.t1 AT2G21200 62.500 72 26 1 15 85 15 86 1.49e-28 98.6
MS.gene057790.t1 AT5G18030 59.494 79 28 2 9 86 13 88 1.49e-28 98.6
MS.gene057790.t1 AT4G34810 61.728 81 26 2 10 85 37 117 3.78e-28 98.6
MS.gene057790.t1 AT5G18010 56.962 79 32 2 9 86 13 90 1.70e-27 95.9
MS.gene057790.t1 AT4G38850 54.878 82 29 2 8 86 13 89 1.04e-26 94.0
MS.gene057790.t1 AT4G34770 55.952 84 35 1 5 86 19 102 2.52e-26 93.2
MS.gene057790.t1 AT3G03840 56.410 78 32 1 11 86 17 94 3.18e-26 92.8
MS.gene057790.t1 AT3G03820 54.430 79 33 1 11 86 17 95 2.12e-25 90.9
MS.gene057790.t1 AT4G13790 48.235 85 41 2 3 85 8 91 2.73e-25 90.5
MS.gene057790.t1 AT3G03850 62.687 67 23 1 22 86 26 92 8.76e-25 89.0
MS.gene057790.t1 AT3G03830 54.545 77 32 1 13 86 16 92 3.38e-24 87.4
MS.gene057790.t1 AT4G34790 60.000 65 24 1 21 83 39 103 2.10e-23 85.9
MS.gene057790.t1 AT4G34780 57.143 70 27 1 19 85 26 95 4.72e-22 82.4
MS.gene057790.t1 AT4G38860 51.471 68 33 0 19 86 38 105 1.06e-20 79.0
MS.gene057790.t1 AT2G21220 50.000 68 33 1 19 86 38 104 4.11e-19 75.1
MS.gene057790.t1 AT1G75580 53.731 67 30 1 19 85 42 107 4.50e-19 75.1
MS.gene057790.t1 AT4G34760 50.746 67 32 1 19 85 41 106 1.21e-18 73.9
MS.gene057790.t1 AT3G43120 54.237 59 26 1 20 78 78 135 9.03e-18 73.2
MS.gene057790.t1 AT2G16580 49.231 65 32 1 19 83 42 105 2.32e-17 70.9
MS.gene057790.t1 AT4G36110 42.500 80 45 1 4 83 23 101 2.64e-17 70.5
MS.gene057790.t1 AT5G20810 54.237 59 26 1 20 78 78 135 1.28e-16 70.1
MS.gene057790.t1 AT5G20810 54.237 59 26 1 20 78 78 135 1.92e-16 70.5
MS.gene057790.t1 AT1G19830 52.941 68 30 2 19 85 46 112 2.75e-16 68.2
MS.gene057790.t1 AT2G18010 48.438 64 32 1 20 83 47 109 3.00e-16 68.2
MS.gene057790.t1 AT3G20220 50.769 65 30 2 21 84 47 110 1.88e-15 66.2
MS.gene057790.t1 AT5G66260 46.970 66 34 1 21 86 34 98 3.62e-14 62.4
MS.gene057790.t1 AT2G37030 47.619 63 32 1 20 82 48 109 1.46e-13 61.6
MS.gene057790.t1 AT1G75590 46.575 73 36 2 10 80 29 100 2.25e-13 61.6
MS.gene057790.t1 AT3G51200 41.463 82 42 3 5 81 19 99 3.22e-13 60.1
MS.gene057790.t1 AT2G46690 42.857 77 40 2 5 78 8 83 3.31e-13 60.5
MS.gene057790.t1 AT5G10990 44.156 77 37 2 4 80 29 99 7.72e-13 60.1
MS.gene057790.t1 AT3G53250 44.444 63 31 1 20 82 51 109 1.71e-12 58.5
MS.gene057790.t1 AT3G53250 44.444 63 31 1 20 82 82 140 2.40e-12 58.9
MS.gene057790.t1 AT1G19840 45.455 77 36 2 4 80 29 99 2.87e-12 58.9
MS.gene057790.t1 AT4G31320 45.588 68 36 1 14 81 73 139 3.65e-12 59.3
MS.gene057790.t1 AT4G12410 45.588 68 33 2 21 85 76 142 4.97e-12 58.2
MS.gene057790.t1 AT4G34750 42.188 64 36 1 20 83 36 98 6.38e-12 57.8
MS.gene057790.t1 AT4G34750 42.188 64 36 1 20 83 36 98 6.38e-12 57.8
MS.gene057790.t1 AT4G22620 40.789 76 41 2 13 85 71 145 1.21e-11 57.4
MS.gene057790.t1 AT4G00880 42.308 78 39 3 3 78 13 86 2.24e-11 55.8
MS.gene057790.t1 AT2G45210 38.889 72 40 2 17 85 75 145 2.35e-11 56.6
MS.gene057790.t1 AT2G24400 49.180 61 30 1 21 81 64 123 2.97e-11 56.6
MS.gene057790.t1 AT5G03310 47.059 68 29 3 20 81 38 104 4.35e-11 54.7

Find 16 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCTTGGCTTAACAATTCT+TGG 0.200185 4.4:-36651282 None:intergenic
ACAATTCTTGGAATGAGGTT+TGG 0.397053 4.4:-36651270 None:intergenic
GTTTGGTTCAAGTATGATAC+AGG 0.430787 4.4:-36651253 None:intergenic
GCTTAACAATTCTTGGAATG+AGG 0.457370 4.4:-36651275 None:intergenic
ATTGAATATGATCATCCAAC+GGG 0.473372 4.4:+36651313 MS.gene057790:CDS
TACCAAGTATCATCAGATCA+AGG 0.490956 4.4:+36651149 MS.gene057790:CDS
GCCCTTGATCTGATGATACT+TGG 0.512708 4.4:-36651151 None:intergenic
TACACAGCTAGATATCCCTT+TGG 0.526180 4.4:-36651199 None:intergenic
GGATATCTAGCTGTGTATGT+AGG 0.572626 4.4:+36651205 MS.gene057790:CDS
AAGAAAACGTCTTCTCTGCA+AGG 0.585314 4.4:-36651349 None:intergenic
AAAACTGTGGACGTTCCAAA+GGG 0.591452 4.4:+36651184 MS.gene057790:CDS
AATTGAATATGATCATCCAA+CGG 0.593128 4.4:+36651312 MS.gene057790:CDS
ACCAAGTATCATCAGATCAA+GGG 0.600732 4.4:+36651150 MS.gene057790:CDS
GGAATAGTGAGACCACCCGT+TGG 0.627472 4.4:-36651328 None:intergenic
GAATATGATCATCCAACGGG+TGG 0.646056 4.4:+36651316 MS.gene057790:CDS
AGAATTGTTAAGCCAAGCTG+AGG 0.653696 4.4:+36651285 MS.gene057790:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AATTGAATATGATCATCCAA+CGG + chr4.4:36651312-36651331 MS.gene057790:CDS 25.0%
ATTGAATATGATCATCCAAC+GGG + chr4.4:36651313-36651332 MS.gene057790:CDS 30.0%
!!! ATTCAATTTTTTCCTCAGCT+TGG - chr4.4:36651300-36651319 None:intergenic 30.0%
ACAATTCTTGGAATGAGGTT+TGG - chr4.4:36651273-36651292 None:intergenic 35.0%
ACCAAGTATCATCAGATCAA+GGG + chr4.4:36651150-36651169 MS.gene057790:CDS 35.0%
GCTTAACAATTCTTGGAATG+AGG - chr4.4:36651278-36651297 None:intergenic 35.0%
TACCAAGTATCATCAGATCA+AGG + chr4.4:36651149-36651168 MS.gene057790:CDS 35.0%
! GTTTGGTTCAAGTATGATAC+AGG - chr4.4:36651256-36651275 None:intergenic 35.0%
AAAAACTGTGGACGTTCCAA+AGG + chr4.4:36651183-36651202 MS.gene057790:CDS 40.0%
AAAACTGTGGACGTTCCAAA+GGG + chr4.4:36651184-36651203 MS.gene057790:CDS 40.0%
AAGAAAACGTCTTCTCTGCA+AGG - chr4.4:36651352-36651371 None:intergenic 40.0%
AGAATTGTTAAGCCAAGCTG+AGG + chr4.4:36651285-36651304 MS.gene057790:CDS 40.0%
CAGCTTGGCTTAACAATTCT+TGG - chr4.4:36651285-36651304 None:intergenic 40.0%
GGATATCTAGCTGTGTATGT+AGG + chr4.4:36651205-36651224 MS.gene057790:CDS 40.0%
GGCATCATCTTCAAAAACTG+TGG + chr4.4:36651171-36651190 MS.gene057790:CDS 40.0%
TACACAGCTAGATATCCCTT+TGG - chr4.4:36651202-36651221 None:intergenic 40.0%
GAATATGATCATCCAACGGG+TGG + chr4.4:36651316-36651335 MS.gene057790:CDS 45.0%
GCCCTTGATCTGATGATACT+TGG - chr4.4:36651154-36651173 None:intergenic 45.0%
GGAATAGTGAGACCACCCGT+TGG - chr4.4:36651331-36651350 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 36651136 36651396 36651136 ID=MS.gene057790
chr4.4 mRNA 36651136 36651396 36651136 ID=MS.gene057790.t1;Parent=MS.gene057790
chr4.4 exon 36651136 36651396 36651136 ID=MS.gene057790.t1.exon1;Parent=MS.gene057790.t1
chr4.4 CDS 36651136 36651396 36651136 ID=cds.MS.gene057790.t1;Parent=MS.gene057790.t1
Gene Sequence

>MS.gene057790

ATGGGTTTTCGTTTACCAAGTATCATCAGATCAAGGGCATCATCTTCAAAAACTGTGGACGTTCCAAAGGGATATCTAGCTGTGTATGTAGGAGAGCAAATGAAGCGATTTGTGATTCCTGTATCATACTTGAACCAAACCTCATTCCAAGAATTGTTAAGCCAAGCTGAGGAAAAAATTGAATATGATCATCCAACGGGTGGTCTCACTATTCCTTGCAGAGAAGACGTTTTCTTAGAGATCACTTCTCGTTTGAATTGA

Protein sequence

>MS.gene057790.t1

MGFRLPSIIRSRASSSKTVDVPKGYLAVYVGEQMKRFVIPVSYLNQTSFQELLSQAEEKIEYDHPTGGLTIPCREDVFLEITSRLN