AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene031198

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031198.t1 MTR_3g110032 93.548 93 6 0 1 93 1 93 3.20e-61 181
MS.gene031198.t1 MTR_4g050700 88.172 93 11 0 1 93 1 93 1.43e-58 174
MS.gene031198.t1 MTR_2g012410 88.172 93 11 0 1 93 1 93 1.43e-58 174
MS.gene031198.t1 MTR_3g084150 86.022 93 12 1 1 93 1 92 3.14e-57 171
MS.gene031198.t1 MTR_3g084170 88.172 93 10 1 1 93 1 92 5.50e-57 170
MS.gene031198.t1 MTR_3g084180 84.946 93 13 1 1 93 1 92 3.12e-56 168
MS.gene031198.t1 MTR_3g084240 87.097 93 11 1 1 93 1 92 5.83e-56 168
MS.gene031198.t1 MTR_3g084260 84.946 93 14 0 1 93 1 93 1.40e-55 167
MS.gene031198.t1 MTR_8g461410 82.796 93 15 1 1 93 1 92 1.77e-55 166
MS.gene031198.t1 MTR_3g084220 86.022 93 12 1 1 93 1 92 1.87e-55 166
MS.gene031198.t1 MTR_3g110038 84.946 93 13 1 1 93 1 92 1.89e-54 164
MS.gene031198.t1 MTR_3g117630 81.111 90 17 0 4 93 3 92 8.30e-51 155
MS.gene031198.t1 MTR_4g072880 74.194 93 24 0 1 93 1 93 2.99e-50 153
MS.gene031198.t1 MTR_4g072310 74.194 93 24 0 1 93 1 93 3.30e-50 153
MS.gene031198.t1 MTR_3g110042 81.818 88 15 1 1 88 1 87 1.76e-49 151
MS.gene031198.t1 MTR_4g072250 74.194 93 23 1 1 93 1 92 6.26e-49 150
MS.gene031198.t1 MTR_4g072230 76.344 93 20 1 1 93 1 91 3.22e-48 148
MS.gene031198.t1 MTR_4g072270 70.968 93 27 0 1 93 1 93 7.63e-48 147
MS.gene031198.t1 MTR_4g072330 74.725 91 20 1 1 91 1 88 2.28e-47 146
MS.gene031198.t1 MTR_4g072500 73.118 93 24 1 1 93 1 92 6.72e-47 145
MS.gene031198.t1 MTR_4g072860 70.968 93 27 0 1 93 1 93 3.11e-46 143
MS.gene031198.t1 MTR_4g072370 73.626 91 21 1 1 91 1 88 4.51e-46 143
MS.gene031198.t1 MTR_4g072240 70.330 91 27 0 1 91 1 91 7.53e-46 142
MS.gene031198.t1 MTR_4g072870 69.231 91 28 0 1 91 1 91 1.36e-45 141
MS.gene031198.t1 MTR_4g072740 73.626 91 18 1 1 91 1 85 2.59e-45 140
MS.gene031198.t1 MTR_4g072830 75.556 90 19 1 1 90 1 87 2.79e-45 140
MS.gene031198.t1 MTR_4g072640 72.527 91 24 1 1 91 1 90 6.45e-45 140
MS.gene031198.t1 MTR_3g084250 70.968 93 26 1 1 93 1 92 7.28e-45 140
MS.gene031198.t1 MTR_4g072300 69.231 91 28 0 1 91 1 91 9.81e-45 139
MS.gene031198.t1 MTR_4g072530 71.429 91 25 1 1 91 1 90 1.58e-44 139
MS.gene031198.t1 MTR_4g072470 72.527 91 19 1 1 91 1 85 1.68e-44 139
MS.gene031198.t1 MTR_4g072220 68.817 93 29 0 1 93 1 93 2.53e-44 138
MS.gene031198.t1 MTR_4g072490 70.330 91 21 1 1 91 1 85 6.05e-44 137
MS.gene031198.t1 MTR_4g072480 68.817 93 28 1 1 93 1 92 6.90e-44 137
MS.gene031198.t1 MTR_4g072465 71.429 91 21 1 1 91 1 86 9.99e-44 137
MS.gene031198.t1 MTR_4g072730 69.565 92 27 1 1 92 1 91 1.05e-43 137
MS.gene031198.t1 MTR_4g072680 68.817 93 28 1 1 93 1 92 1.11e-43 137
MS.gene031198.t1 MTR_4g072570 70.968 93 26 1 1 93 1 92 1.12e-43 137
MS.gene031198.t1 MTR_4g072580 71.429 91 20 1 1 91 1 85 2.34e-43 135
MS.gene031198.t1 MTR_4g072620 67.742 93 29 1 1 93 1 92 2.38e-43 136
MS.gene031198.t1 MTR_4g072890 67.033 91 30 0 1 91 1 91 7.00e-43 135
MS.gene031198.t1 MTR_4g072850 74.468 94 23 1 1 93 1 94 7.58e-43 135
MS.gene031198.t1 MTR_4g072260 65.591 93 32 0 1 93 1 93 7.60e-43 135
MS.gene031198.t1 MTR_4g072610 72.527 91 18 2 1 91 1 84 2.38e-42 133
MS.gene031198.t1 MTR_4g072650 73.626 91 17 2 1 91 1 84 2.41e-42 133
MS.gene031198.t1 MTR_4g072460 66.667 93 30 1 1 93 1 92 4.13e-42 133
MS.gene031198.t1 MTR_4g072630 68.132 91 24 1 1 91 1 86 1.23e-41 131
MS.gene031198.t1 MTR_4g072670 70.330 91 22 1 1 91 1 86 4.46e-40 127
MS.gene031198.t1 MTR_3g084210 72.043 93 25 1 1 93 1 92 4.98e-40 127
MS.gene031198.t1 MTR_3g084200 83.562 73 11 1 1 73 1 72 7.20e-40 127
MS.gene031198.t1 MTR_4g072540 69.231 91 23 1 1 91 1 86 1.70e-39 126
MS.gene031198.t1 MTR_4g072720 70.330 91 22 1 1 91 1 86 2.52e-39 125
MS.gene031198.t1 MTR_4g072660 63.441 93 28 1 1 93 1 87 3.57e-39 125
MS.gene031198.t1 MTR_4g072600 69.318 88 21 1 1 88 1 82 4.75e-39 125
MS.gene031198.t1 MTR_3g084190 69.892 93 27 1 1 93 1 92 2.44e-38 123
MS.gene031198.t1 MTR_4g072510 68.132 91 24 1 1 91 1 86 3.55e-38 122
MS.gene031198.t1 MTR_8g461420 70.330 91 26 1 1 91 1 90 5.56e-38 122
MS.gene031198.t1 MTR_4g072820 67.391 92 22 3 1 91 1 85 6.42e-38 122
MS.gene031198.t1 MTR_4g072590 64.835 91 26 1 1 91 1 85 2.15e-37 120
MS.gene031198.t1 MTR_3g084230 69.231 91 27 1 1 91 1 90 3.15e-37 120
MS.gene031198.t1 MTR_4g072380 67.391 92 23 2 1 91 1 86 3.42e-37 120
MS.gene031198.t1 MTR_5g008370 66.316 95 25 2 1 92 1 91 6.87e-37 121
MS.gene031198.t1 MTR_5g008360 72.368 76 21 0 18 93 8 83 8.01e-37 119
MS.gene031198.t1 MTR_4g072940 68.478 92 28 1 3 93 33 124 8.45e-37 120
MS.gene031198.t1 MTR_3g084160 66.667 93 30 1 1 93 1 92 1.24e-36 119
MS.gene031198.t1 MTR_3g110045 70.330 91 26 1 3 93 62 151 2.08e-36 120
MS.gene031198.t1 MTR_5g008350 68.750 80 19 1 1 80 1 74 6.68e-36 117
MS.gene031198.t1 MTR_4g072690 63.736 91 27 1 1 91 1 85 1.22e-35 116
MS.gene031198.t1 MTR_4g072550 61.957 92 33 1 4 93 12 103 5.00e-35 115
MS.gene031198.t1 MTR_4g072450 63.736 91 27 1 1 91 1 85 5.01e-35 115
MS.gene031198.t1 MTR_4g072980 54.737 95 41 2 1 93 1 95 6.76e-34 112
MS.gene031198.t1 MTR_8g461400 65.591 93 5 3 1 93 1 66 8.19e-33 108
MS.gene031198.t1 MTR_4g072290 58.065 93 25 1 1 93 1 79 3.66e-32 107
MS.gene031198.t1 MTR_4g072770 74.242 66 17 0 26 91 15 80 7.48e-32 107
MS.gene031198.t1 MTR_4g072560 51.546 97 40 2 1 90 1 97 1.96e-31 106
MS.gene031198.t1 MTR_3g117640 52.475 101 39 2 1 92 1 101 8.99e-31 104
MS.gene031198.t1 MTR_4g072910 67.164 67 22 0 25 91 34 100 4.08e-30 103
MS.gene031198.t1 MTR_4g072280 64.000 75 27 0 1 75 1 75 5.05e-29 99.8
MS.gene031198.t1 MTR_4g072750 67.568 74 21 2 1 72 1 73 4.71e-28 97.4
MS.gene031198.t1 MTR_3g110035 66.667 66 22 0 28 93 73 138 1.71e-27 97.4
MS.gene031198.t1 MTR_4g072930 48.235 85 40 1 11 91 26 110 3.19e-24 88.6
MS.gene031198.t1 MTR_3g113310 54.688 64 28 1 25 88 41 103 1.05e-21 82.0
MS.gene031198.t1 MTR_6g077850 50.000 68 34 0 24 91 32 99 1.86e-21 81.3
MS.gene031198.t1 MTR_3g117610 57.812 64 26 1 25 88 44 106 2.23e-21 81.3
MS.gene031198.t1 MTR_4g072190 51.351 74 35 1 15 88 25 97 6.59e-21 79.7
MS.gene031198.t1 MTR_5g021820 47.561 82 39 2 10 88 21 101 7.48e-20 77.0
MS.gene031198.t1 MTR_4g072840 47.253 91 17 3 1 91 1 60 2.27e-19 74.7
MS.gene031198.t1 MTR_8g076040 46.341 82 40 2 10 88 22 102 2.33e-19 75.9
MS.gene031198.t1 MTR_2g043960 50.704 71 34 1 16 86 66 135 5.67e-19 76.6
MS.gene031198.t1 MTR_4g124750 47.674 86 43 2 4 88 46 130 1.83e-18 75.1
MS.gene031198.t1 MTR_1g094750 47.059 85 41 2 5 86 26 109 7.50e-18 72.4
MS.gene031198.t1 MTR_4g005310 46.575 73 38 1 15 86 51 123 1.36e-17 72.8
MS.gene031198.t1 MTR_1g009150 49.383 81 36 2 10 86 51 130 1.59e-17 73.2
MS.gene031198.t1 MTR_4g005320 54.098 61 27 1 26 86 70 129 3.87e-16 69.3
MS.gene031198.t1 MTR_3g109160 44.578 83 45 1 10 92 53 134 8.95e-15 65.9
MS.gene031198.t1 MTR_3g105900 73.171 41 11 0 25 65 1171 1211 1.01e-14 68.2
MS.gene031198.t1 MTR_8g461210 43.023 86 42 2 4 83 7 91 1.35e-14 64.3
MS.gene031198.t1 MTR_1g024085 42.683 82 44 2 4 83 13 93 4.77e-14 63.5
MS.gene031198.t1 MTR_3g092220 44.928 69 35 2 17 83 24 91 5.56e-14 62.4
MS.gene031198.t1 MTR_7g118010 49.206 63 29 2 26 86 23 84 1.16e-13 61.6
MS.gene031198.t1 MTR_1g061570 46.377 69 36 1 24 92 17 84 2.12e-13 61.2
MS.gene031198.t1 MTR_0388s0020 40.741 81 43 3 11 88 61 139 6.27e-13 60.5
MS.gene031198.t1 MTR_4g072160 47.143 70 34 2 18 86 29 96 6.53e-13 60.5
MS.gene031198.t1 MTR_8g026730 46.032 63 31 2 26 86 27 88 1.65e-12 58.5
MS.gene031198.t1 MTR_7g101740 44.615 65 35 1 24 88 38 101 6.32e-12 57.4
MS.gene031198.t1 MTR_4g021810 52.542 59 26 2 26 83 26 83 1.10e-11 56.2
MS.gene031198.t1 MTR_8g101520 37.500 88 50 2 8 91 28 114 2.18e-11 56.6
MS.gene031198.t1 MTR_7g405740 44.262 61 33 1 26 86 67 126 5.52e-11 55.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031198.t1 AT4G38840 58.586 99 33 2 1 91 1 99 3.32e-36 118
MS.gene031198.t1 AT5G18020 64.474 76 26 1 17 91 15 90 4.59e-34 112
MS.gene031198.t1 AT5G18060 60.526 76 29 1 17 91 15 90 1.12e-31 106
MS.gene031198.t1 AT5G18080 60.526 76 29 1 17 91 15 90 1.79e-31 106
MS.gene031198.t1 AT5G18050 59.211 76 30 1 17 91 15 90 2.63e-31 105
MS.gene031198.t1 AT5G18030 62.500 72 26 1 21 91 17 88 4.29e-31 105
MS.gene031198.t1 AT4G38825 60.000 75 29 1 18 91 15 89 8.52e-31 104
MS.gene031198.t1 AT3G03840 64.384 73 24 1 22 92 23 95 1.26e-30 104
MS.gene031198.t1 AT4G34800 53.763 93 35 2 1 90 1 88 2.33e-30 103
MS.gene031198.t1 AT5G18010 57.895 76 31 1 17 91 15 90 4.63e-30 102
MS.gene031198.t1 AT4G34770 60.241 83 31 1 12 92 21 103 1.02e-29 102
MS.gene031198.t1 AT2G21200 62.500 72 26 1 20 90 15 86 1.13e-29 101
MS.gene031198.t1 AT2G21210 58.108 74 29 1 20 91 22 95 7.37e-29 99.8
MS.gene031198.t1 AT3G03820 53.409 88 38 1 8 92 9 96 1.02e-28 99.4
MS.gene031198.t1 AT3G03850 66.176 68 21 1 27 92 26 93 1.66e-28 99.0
MS.gene031198.t1 AT2G21210 58.108 74 29 1 20 91 89 162 4.92e-28 99.8
MS.gene031198.t1 AT4G38850 57.534 73 28 1 22 91 17 89 7.10e-28 97.1
MS.gene031198.t1 AT4G34810 63.235 68 21 1 25 88 47 114 1.19e-27 97.4
MS.gene031198.t1 AT3G03830 50.549 91 38 2 8 91 2 92 2.65e-26 93.2
MS.gene031198.t1 AT4G13790 50.602 83 34 2 9 89 13 90 1.33e-24 89.0
MS.gene031198.t1 AT4G34780 61.429 70 24 1 25 91 27 96 7.74e-24 87.4
MS.gene031198.t1 AT4G34790 60.000 65 24 1 26 88 39 103 1.10e-23 87.0
MS.gene031198.t1 AT4G38860 56.250 64 28 0 25 88 39 102 1.49e-21 81.6
MS.gene031198.t1 AT1G75580 59.375 64 25 1 25 88 43 105 4.89e-21 80.5
MS.gene031198.t1 AT4G34760 57.812 64 26 1 25 88 42 104 6.26e-21 80.1
MS.gene031198.t1 AT4G36110 48.649 74 37 1 17 90 31 103 3.94e-20 77.8
MS.gene031198.t1 AT2G21220 52.308 65 30 1 24 88 38 101 1.74e-19 76.3
MS.gene031198.t1 AT3G43120 47.222 72 37 1 15 86 68 138 3.35e-19 77.0
MS.gene031198.t1 AT2G16580 54.688 64 28 1 25 88 43 105 4.13e-19 75.5
MS.gene031198.t1 AT2G18010 47.297 74 38 1 17 90 39 111 9.92e-19 74.7
MS.gene031198.t1 AT5G20810 49.296 71 35 1 16 86 69 138 3.63e-18 74.7
MS.gene031198.t1 AT5G20810 49.296 71 35 1 16 86 69 138 4.87e-18 74.7
MS.gene031198.t1 AT3G20220 48.000 75 37 2 17 90 38 111 9.61e-18 72.4
MS.gene031198.t1 AT1G19830 55.932 59 25 1 25 83 47 104 6.27e-17 70.1
MS.gene031198.t1 AT1G75590 46.429 84 40 2 4 83 16 98 6.35e-17 71.2
MS.gene031198.t1 AT5G10990 47.945 73 36 2 11 83 27 97 1.08e-15 67.8
MS.gene031198.t1 AT5G66260 40.449 89 50 2 5 91 11 98 8.73e-15 64.3
MS.gene031198.t1 AT4G31320 51.515 66 31 1 21 86 75 139 1.94e-14 65.5
MS.gene031198.t1 AT2G24400 53.030 66 30 1 21 86 59 123 2.71e-14 64.7
MS.gene031198.t1 AT1G19840 47.059 68 35 1 16 83 31 97 3.26e-14 63.9
MS.gene031198.t1 AT2G46690 39.744 78 43 2 18 92 16 92 4.39e-14 63.2
MS.gene031198.t1 AT3G51200 45.070 71 37 2 15 84 28 97 1.46e-13 61.2
MS.gene031198.t1 AT3G61900 39.286 84 47 2 11 91 15 97 3.20e-13 61.2
MS.gene031198.t1 AT2G37030 41.667 72 41 1 16 87 39 109 6.97e-13 60.1
MS.gene031198.t1 AT2G45210 40.278 72 39 2 22 90 75 145 1.57e-12 60.1
MS.gene031198.t1 AT4G34750 37.975 79 47 2 10 88 22 98 2.44e-12 59.3
MS.gene031198.t1 AT4G34750 37.975 79 47 2 10 88 22 98 2.44e-12 59.3
MS.gene031198.t1 AT5G53590 33.333 93 56 2 1 88 21 112 3.40e-12 58.5
MS.gene031198.t1 AT3G12830 39.437 71 42 1 16 86 40 109 3.92e-12 58.2
MS.gene031198.t1 AT3G60690 39.726 73 40 2 22 91 84 155 6.01e-12 58.5
MS.gene031198.t1 AT4G00880 50.000 64 29 2 25 86 27 89 1.04e-11 57.0
MS.gene031198.t1 AT3G03847 40.000 85 48 3 3 86 48 130 2.43e-11 56.2

Find 0 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAAAAAACATCTTCTGTGCA+AGG + 21207:16394-16413 None:intergenic 30.0%
! ACAAGTCTTGTAATGAAGTT+TGG + 21207:16315-16334 None:intergenic 30.0%
! TAATGAAGTTTGGTTCAAGT+AGG + 21207:16305-16324 None:intergenic 30.0%
ATATCACTTCTCGCTTCAAT+GGG - 21207:16416-16435 MS.gene031198:CDS 35.0%
ATTCGGATATGATCATCCAA+TGG - 21207:16354-16373 MS.gene031198:CDS 35.0%
TTCGGATATGATCATCCAAT+GGG - 21207:16355-16374 MS.gene031198:CDS 35.0%
TTCTTATAGCTGCTGGTAAA+CGG + 21207:16174-16193 None:intergenic 35.0%
TTTACCAGCAGCTATAAGAA+GGG - 21207:16174-16193 MS.gene031198:CDS 35.0%
! TTTGGTTCAAGTAGGACATA+GGG + 21207:16297-16316 None:intergenic 35.0%
AAAAGCGACAAATGTGCCAA+AGG - 21207:16225-16244 MS.gene031198:CDS 40.0%
AAAGCGACAAATGTGCCAAA+GGG - 21207:16226-16245 MS.gene031198:CDS 40.0%
CATATCACTTCTCGCTTCAA+TGG - 21207:16415-16434 MS.gene031198:CDS 40.0%
GTTTACCAGCAGCTATAAGA+AGG - 21207:16173-16192 MS.gene031198:CDS 40.0%
! GTTTGGTTCAAGTAGGACAT+AGG + 21207:16298-16317 None:intergenic 40.0%
!!! TTGTCGCTTTTGTTGATGCT+TGG + 21207:16216-16235 None:intergenic 40.0%
ACGTTGGAGAGGAAATGAAG+CGG - 21207:16263-16282 MS.gene031198:CDS 45.0%
AGTCGAGCAGAGGAAGAATT+CGG - 21207:16337-16356 MS.gene031198:CDS 45.0%
CTTCTGTGCAAGGAATTGTG+AGG + 21207:16384-16403 None:intergenic 45.0%
GGATATGATCATCCAATGGG+TGG - 21207:16358-16377 MS.gene031198:CDS 45.0%
TACACTGCAAGATAGCCCTT+TGG + 21207:16244-16263 None:intergenic 45.0%
TGATGCTTGGCTACTGCTAA+AGG + 21207:16203-16222 None:intergenic 45.0%
GAAGCCCTTCTTATAGCTGC+TGG + 21207:16181-16200 None:intergenic 50.0%
GGCTATCTTGCAGTGTACGT+TGG - 21207:16247-16266 MS.gene031198:CDS 50.0%
TCTTGCAGTGTACGTTGGAG+AGG - 21207:16252-16271 MS.gene031198:CDS 50.0%
!! AGACTTGTTGAGTCGAGCAG+AGG - 21207:16327-16346 MS.gene031198:CDS 50.0%
GGAATTGTGAGGCCACCCAT+TGG + 21207:16373-16392 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
21207 gene 16163 16444 16163 ID=MS.gene031198
21207 mRNA 16163 16444 16163 ID=MS.gene031198.t1;Parent=MS.gene031198
21207 exon 16163 16444 16163 ID=MS.gene031198.t1.exon1;Parent=MS.gene031198.t1
21207 CDS 16163 16444 16163 ID=cds.MS.gene031198.t1;Parent=MS.gene031198.t1
Gene Sequence

>MS.gene031198

ATGGGCTTCCGTTTACCAGCAGCTATAAGAAGGGCTTCCTTTAGCAGTAGCCAAGCATCAACAAAAGCGACAAATGTGCCAAAGGGCTATCTTGCAGTGTACGTTGGAGAGGAAATGAAGCGGTTTGTGATCCCTATGTCCTACTTGAACCAAACTTCATTACAAGACTTGTTGAGTCGAGCAGAGGAAGAATTCGGATATGATCATCCAATGGGTGGCCTCACAATTCCTTGCACAGAAGATGTTTTTTTGCATATCACTTCTCGCTTCAATGGGCTGTAA
Protein sequence

>MS.gene031198.t1

MGFRLPAAIRRASFSSSQASTKATNVPKGYLAVYVGEEMKRFVIPMSYLNQTSLQDLLSRAEEEFGYDHPMGGLTIPCTEDVFLHITSRFNGL