AlfalfaGEDB | MS.gene40326

M. sativa cultivar XinJiangDaYe / MS.gene40326

KEGG: K14488
GO: GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
PFAM: PF02519.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene40326.t1	RHN70824.1	96.7	92	3	0	1	92	59	150	1.10E-42	182.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene40326.t1	P33080	67.7	93	28	2	1	92	1	92	3.2e-30	132.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene40326.t1	G7ZWL0	96.7	92	3	0	1	92	1	92	8.0e-43	182.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049159	MS.gene40326	0.809903	1.50E-50	-1.69E-46
MS.gene049378	MS.gene40326	0.810476	1.13E-50	-1.69E-46
MS.gene050442	MS.gene40326	0.803517	3.36E-49	-1.69E-46
MS.gene050623	MS.gene40326	-0.806295	8.82E-50	-1.69E-46
MS.gene050780	MS.gene40326	0.802185	6.32E-49	-1.69E-46
MS.gene050815	MS.gene40326	0.824149	9.44E-54	-1.69E-46
MS.gene050826	MS.gene40326	0.839186	1.84E-57	-1.69E-46
MS.gene051719	MS.gene40326	0.80414	2.49E-49	-1.69E-46
MS.gene052519	MS.gene40326	0.802127	6.50E-49	-1.69E-46
MS.gene052673	MS.gene40326	-0.803198	3.91E-49	-1.69E-46
MS.gene052773	MS.gene40326	0.824795	6.65E-54	-1.69E-46
MS.gene052863	MS.gene40326	0.803151	4.00E-49	-1.69E-46
MS.gene053008	MS.gene40326	0.814587	1.43E-51	-1.69E-46
MS.gene05415	MS.gene40326	0.812541	4.02E-51	-1.69E-46
MS.gene054236	MS.gene40326	0.81752	3.16E-52	-1.69E-46
MS.gene054291	MS.gene40326	-0.802262	6.10E-49	-1.69E-46
MS.gene054379	MS.gene40326	0.805189	1.51E-49	-1.69E-46
MS.gene054468	MS.gene40326	0.817918	2.57E-52	-1.69E-46
MS.gene054472	MS.gene40326	0.802908	4.49E-49	-1.69E-46
MS.gene05468	MS.gene40326	0.806905	6.55E-50	-1.69E-46
MS.gene055227	MS.gene40326	0.803644	3.16E-49	-1.69E-46
MS.gene055623	MS.gene40326	0.804705	1.90E-49	-1.69E-46
MS.gene055701	MS.gene40326	0.819464	1.15E-52	-1.69E-46
MS.gene055818	MS.gene40326	0.823189	1.58E-53	-1.69E-46
MS.gene055926	MS.gene40326	0.840157	1.03E-57	-1.69E-46
MS.gene05604	MS.gene40326	0.806308	8.76E-50	-1.69E-46
MS.gene056506	MS.gene40326	0.801609	8.30E-49	-1.69E-46
MS.gene057026	MS.gene40326	0.814488	1.50E-51	-1.69E-46
MS.gene057060	MS.gene40326	0.817599	3.03E-52	-1.69E-46
MS.gene059161	MS.gene40326	0.804032	2.62E-49	-1.69E-46
MS.gene060137	MS.gene40326	-0.803443	3.48E-49	-1.69E-46
MS.gene060322	MS.gene40326	0.827945	1.18E-54	-1.69E-46
MS.gene060634	MS.gene40326	-0.808047	3.75E-50	-1.69E-46
MS.gene060685	MS.gene40326	0.800741	1.25E-48	-1.69E-46
MS.gene060802	MS.gene40326	0.828278	9.83E-55	-1.69E-46
MS.gene060803	MS.gene40326	0.837189	6.02E-57	-1.69E-46
MS.gene060998	MS.gene40326	0.815101	1.10E-51	-1.69E-46
MS.gene061040	MS.gene40326	-0.803539	3.32E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene40326	MS.gene022358	PPI
MS.gene40326	MS.gene062954	PPI
MS.gene062971	MS.gene40326	PPI
MS.gene23373	MS.gene40326	PPI
MS.gene40326	MS.gene23369	PPI
MS.gene40326	MS.gene062986	PPI
MS.gene40326	MS.gene057783	PPI
MS.gene40326	MS.gene50770	PPI
MS.gene40326	MS.gene062987	PPI
MS.gene40326	MS.gene057811	PPI
MS.gene40326	MS.gene057806	PPI
MS.gene062975	MS.gene40326	PPI
MS.gene40326	MS.gene062964	PPI
MS.gene50689	MS.gene40326	PPI
MS.gene40326	MS.gene77525	PPI
MS.gene40326	MS.gene062944	PPI
MS.gene40326	MS.gene50658	PPI
MS.gene003576	MS.gene40326	PPI
MS.gene40326	MS.gene057777	PPI
MS.gene40326	MS.gene28863	PPI
MS.gene40326	MS.gene062945	PPI
MS.gene40326	MS.gene057804	PPI
MS.gene40326	MS.gene50651	PPI
MS.gene40326	MS.gene50656	PPI
MS.gene40326	MS.gene062969	PPI
MS.gene40326	MS.gene50686	PPI
MS.gene062970	MS.gene40326	PPI
MS.gene40326	MS.gene70683	PPI
MS.gene40326	MS.gene062962	PPI
MS.gene062942	MS.gene40326	PPI
MS.gene40326	MS.gene50666	PPI
MS.gene40326	MS.gene062985	PPI
MS.gene40326	MS.gene057784	PPI
MS.gene40326	MS.gene062953	PPI
MS.gene40326	MS.gene003622	PPI
MS.gene062972	MS.gene40326	PPI
MS.gene40326	MS.gene003599	PPI
MS.gene40326	MS.gene23368	PPI
MS.gene40326	MS.gene003585	PPI
MS.gene003580	MS.gene40326	PPI
MS.gene40326	MS.gene057817	PPI
MS.gene40326	MS.gene50668	PPI
MS.gene40326	MS.gene003581	PPI
MS.gene40326	MS.gene70684	PPI
MS.gene003582	MS.gene40326	PPI
MS.gene40326	MS.gene062958	PPI
MS.gene40326	MS.gene057798	PPI
MS.gene40326	MS.gene003612	PPI
MS.gene40326	MS.gene062983	PPI
MS.gene40326	MS.gene062967	PPI
MS.gene40326	MS.gene003579	PPI
MS.gene40326	MS.gene062940	PPI
MS.gene50772	MS.gene40326	PPI
MS.gene40326	MS.gene062989	PPI
MS.gene40326	MS.gene003595	PPI
MS.gene057809	MS.gene40326	PPI
MS.gene40326	MS.gene062959	PPI
MS.gene40326	MS.gene003607	PPI
MS.gene40326	MS.gene003606	PPI
MS.gene40326	MS.gene50664	PPI
MS.gene40326	MS.gene057785	PPI
MS.gene062978	MS.gene40326	PPI
MS.gene40326	MS.gene50670	PPI
MS.gene40326	MS.gene50669	PPI
MS.gene40326	MS.gene70685	PPI
MS.gene003593	MS.gene40326	PPI
MS.gene40326	MS.gene062941	PPI
MS.gene40326	MS.gene50662	PPI
MS.gene40326	MS.gene50660	PPI
MS.gene40326	MS.gene003623	PPI
MS.gene40326	MS.gene057799	PPI
MS.gene40326	MS.gene003628	PPI
MS.gene40326	MS.gene057794	PPI
MS.gene40326	MS.gene50688	PPI
MS.gene40326	MS.gene003629	PPI
MS.gene40326	MS.gene003603	PPI
MS.gene40326	MS.gene003630	PPI
MS.gene40326	MS.gene062966	PPI
MS.gene40326	MS.gene062980	PPI
MS.gene40326	MS.gene23367	PPI
MS.gene057816	MS.gene40326	PPI
MS.gene40326	MS.gene003610	PPI
MS.gene40326	MS.gene057802	PPI
MS.gene40326	MS.gene50773	PPI
MS.gene40326	MS.gene057796	PPI
MS.gene40326	MS.gene50682	PPI
MS.gene40326	MS.gene057800	PPI
MS.gene40326	MS.gene50653	PPI
MS.gene50657	MS.gene40326	PPI
MS.gene40326	MS.gene003597	PPI
MS.gene40326	MS.gene003598	PPI
MS.gene40326	MS.gene50690	PPI
MS.gene40326	MS.gene057780	PPI
MS.gene40326	MS.gene057788	PPI
MS.gene50683	MS.gene40326	PPI
MS.gene057807	MS.gene40326	PPI
MS.gene40326	MS.gene50655	PPI
MS.gene40326	MS.gene50685	PPI
MS.gene40326	MS.gene003613	PPI
MS.gene40326	MS.gene003584	PPI
MS.gene40326	MS.gene057791	PPI
MS.gene40326	MS.gene50674	PPI
MS.gene003626	MS.gene40326	PPI
MS.gene40326	MS.gene057782	PPI
MS.gene40326	MS.gene057779	PPI
MS.gene40326	MS.gene50673	PPI
MS.gene062946	MS.gene40326	PPI
MS.gene057805	MS.gene40326	PPI
MS.gene40326	MS.gene062965	PPI
MS.gene40326	MS.gene50771	PPI
MS.gene003605	MS.gene40326	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene40326.t1	MTR_3g110038	96.739	92	3	0	1	92	1	92	2.01e-63	186
MS.gene40326.t1	MTR_3g084170	93.478	92	6	0	1	92	1	92	2.34e-61	181
MS.gene40326.t1	MTR_3g084240	92.391	92	7	0	1	92	1	92	2.01e-60	179
MS.gene40326.t1	MTR_3g084220	91.304	92	8	0	1	92	1	92	1.40e-59	177
MS.gene40326.t1	MTR_3g084150	84.946	93	12	2	1	92	1	92	1.35e-54	164
MS.gene40326.t1	MTR_4g050700	83.871	93	14	1	1	92	1	93	2.46e-54	164
MS.gene40326.t1	MTR_2g012410	83.871	93	14	1	1	92	1	93	2.46e-54	164
MS.gene40326.t1	MTR_8g461410	83.871	93	13	2	1	92	1	92	2.91e-54	163
MS.gene40326.t1	MTR_3g084180	83.871	93	13	2	1	92	1	92	1.23e-53	162
MS.gene40326.t1	MTR_3g110032	81.720	93	16	1	1	92	1	93	6.79e-52	157
MS.gene40326.t1	MTR_3g084260	82.418	91	15	1	1	90	1	91	5.18e-51	155
MS.gene40326.t1	MTR_3g110042	82.955	88	13	2	1	87	1	87	4.58e-49	150
MS.gene40326.t1	MTR_3g117630	78.889	90	18	1	4	92	3	92	1.30e-48	149
MS.gene40326.t1	MTR_4g072250	74.194	93	22	2	1	92	1	92	4.90e-47	145
MS.gene40326.t1	MTR_4g072310	72.043	93	25	1	1	92	1	93	5.28e-47	145
MS.gene40326.t1	MTR_4g072880	70.968	93	26	1	1	92	1	93	1.34e-46	144
MS.gene40326.t1	MTR_4g072270	72.043	93	25	1	1	92	1	93	2.59e-46	143
MS.gene40326.t1	MTR_4g072870	71.429	91	25	1	1	90	1	91	1.61e-45	141
MS.gene40326.t1	MTR_4g072370	74.444	90	21	1	1	90	1	88	1.62e-45	141
MS.gene40326.t1	MTR_4g072330	73.333	90	22	1	1	90	1	88	3.19e-45	140
MS.gene40326.t1	MTR_3g084200	90.278	72	7	0	1	72	1	72	1.14e-44	139
MS.gene40326.t1	MTR_4g072240	71.429	91	25	1	1	90	1	91	1.86e-44	139
MS.gene40326.t1	MTR_4g072860	72.043	93	25	1	1	92	1	93	1.98e-44	139
MS.gene40326.t1	MTR_4g072220	70.968	93	26	1	1	92	1	93	2.43e-44	138
MS.gene40326.t1	MTR_4g072260	69.892	93	27	1	1	92	1	93	3.06e-44	138
MS.gene40326.t1	MTR_4g072740	72.222	90	20	1	1	90	1	85	3.91e-44	137
MS.gene40326.t1	MTR_4g072300	71.429	91	25	1	1	90	1	91	6.08e-44	137
MS.gene40326.t1	MTR_4g072230	72.043	93	23	2	1	92	1	91	6.36e-44	137
MS.gene40326.t1	MTR_4g072470	72.222	90	20	1	1	90	1	85	8.26e-44	137
MS.gene40326.t1	MTR_4g072830	75.281	89	20	1	1	89	1	87	1.22e-43	136
MS.gene40326.t1	MTR_4g072890	71.429	91	25	1	1	90	1	91	1.33e-43	136
MS.gene40326.t1	MTR_4g072490	70.000	90	22	1	1	90	1	85	1.54e-43	136
MS.gene40326.t1	MTR_4g072580	71.111	90	21	1	1	90	1	85	2.08e-43	136
MS.gene40326.t1	MTR_4g072640	72.826	92	23	2	1	91	1	91	6.35e-43	135
MS.gene40326.t1	MTR_4g072500	70.968	93	25	2	1	92	1	92	7.90e-43	134
MS.gene40326.t1	MTR_4g072850	71.277	94	25	1	1	92	1	94	1.40e-42	134
MS.gene40326.t1	MTR_4g072630	70.000	90	23	1	1	90	1	86	3.32e-42	133
MS.gene40326.t1	MTR_4g072530	70.330	91	25	2	1	90	1	90	1.04e-41	132
MS.gene40326.t1	MTR_4g072730	66.667	90	30	0	1	90	1	90	1.06e-41	132
MS.gene40326.t1	MTR_4g072610	71.111	90	20	2	1	90	1	84	1.32e-41	131
MS.gene40326.t1	MTR_4g072480	65.217	92	32	0	1	92	1	92	1.69e-41	131
MS.gene40326.t1	MTR_4g072620	64.130	92	33	0	1	92	1	92	5.22e-41	130
MS.gene40326.t1	MTR_3g084250	67.742	93	28	2	1	92	1	92	5.95e-41	130
MS.gene40326.t1	MTR_4g072680	65.217	92	32	0	1	92	1	92	1.04e-40	129
MS.gene40326.t1	MTR_4g072465	66.667	90	26	1	1	90	1	86	1.09e-40	129
MS.gene40326.t1	MTR_4g072650	71.111	90	20	2	1	90	1	84	1.20e-40	129
MS.gene40326.t1	MTR_4g072570	65.217	92	32	0	1	92	1	92	2.17e-40	128
MS.gene40326.t1	MTR_4g072670	68.889	90	24	1	1	90	1	86	1.27e-39	126
MS.gene40326.t1	MTR_4g072540	71.111	90	22	1	1	90	1	86	1.30e-39	126
MS.gene40326.t1	MTR_4g072660	65.217	92	27	1	1	92	1	87	3.87e-39	125
MS.gene40326.t1	MTR_4g072460	63.043	92	34	0	1	92	1	92	3.93e-39	125
MS.gene40326.t1	MTR_4g072600	68.966	87	22	1	1	87	1	82	4.93e-39	125
MS.gene40326.t1	MTR_4g072820	67.778	90	24	2	1	90	1	85	1.05e-38	124
MS.gene40326.t1	MTR_4g072510	68.889	90	24	1	1	90	1	86	1.13e-38	124
MS.gene40326.t1	MTR_4g072940	69.565	92	26	1	3	92	33	124	8.15e-38	123
MS.gene40326.t1	MTR_3g084210	63.043	92	34	0	1	92	1	92	9.77e-38	122
MS.gene40326.t1	MTR_4g072380	67.778	90	25	1	1	90	1	86	1.31e-37	121
MS.gene40326.t1	MTR_3g084190	63.043	92	34	0	1	92	1	92	1.42e-37	121
MS.gene40326.t1	MTR_4g072720	66.667	90	26	1	1	90	1	86	2.93e-37	120
MS.gene40326.t1	MTR_4g072590	67.778	90	24	1	1	90	1	85	4.27e-37	120
MS.gene40326.t1	MTR_8g461420	60.870	92	36	0	1	92	1	92	8.38e-37	119
MS.gene40326.t1	MTR_3g084230	60.870	92	36	0	1	92	1	92	2.48e-36	118
MS.gene40326.t1	MTR_3g084160	61.957	92	35	0	1	92	1	92	4.44e-36	117
MS.gene40326.t1	MTR_5g008350	69.620	79	19	1	1	79	1	74	5.75e-36	117
MS.gene40326.t1	MTR_4g072690	67.778	90	24	1	1	90	1	85	6.84e-36	117
MS.gene40326.t1	MTR_3g110045	63.333	90	33	0	3	92	62	151	1.39e-35	118
MS.gene40326.t1	MTR_5g008360	71.622	74	21	0	19	92	10	83	1.42e-35	116
MS.gene40326.t1	MTR_8g461400	66.304	92	5	1	1	92	1	66	1.55e-35	115
MS.gene40326.t1	MTR_5g008370	63.333	90	33	0	1	90	1	90	4.89e-35	116
MS.gene40326.t1	MTR_4g072450	65.556	90	26	1	1	90	1	85	4.09e-34	112
MS.gene40326.t1	MTR_4g072980	58.696	92	35	2	1	89	1	92	4.43e-34	112
MS.gene40326.t1	MTR_4g072550	60.870	92	33	1	4	92	12	103	6.80e-34	112
MS.gene40326.t1	MTR_4g072560	52.041	98	39	3	1	90	1	98	5.95e-31	105
MS.gene40326.t1	MTR_4g072770	69.697	66	20	0	25	90	15	80	2.68e-30	102
MS.gene40326.t1	MTR_4g072910	63.380	71	26	0	20	90	30	100	5.50e-30	102
MS.gene40326.t1	MTR_3g117640	50.000	100	40	2	1	90	1	100	1.66e-29	101
MS.gene40326.t1	MTR_4g072290	55.435	92	28	2	1	92	1	79	1.70e-28	98.2
MS.gene40326.t1	MTR_4g072280	64.000	75	26	1	1	74	1	75	5.90e-27	94.4
MS.gene40326.t1	MTR_3g110035	63.636	66	24	0	27	92	73	138	8.80e-26	93.2
MS.gene40326.t1	MTR_4g072750	62.667	75	24	2	1	72	1	74	5.71e-25	89.7
MS.gene40326.t1	MTR_3g113310	56.923	65	27	1	23	87	40	103	8.66e-24	87.0
MS.gene40326.t1	MTR_4g072930	47.059	85	40	2	11	90	26	110	3.69e-23	85.9
MS.gene40326.t1	MTR_3g117610	58.462	65	26	1	23	87	43	106	6.21e-23	85.1
MS.gene40326.t1	MTR_6g077850	52.239	67	32	0	24	90	33	99	2.22e-22	83.6
MS.gene40326.t1	MTR_4g072190	48.193	83	41	2	5	87	17	97	4.23e-22	82.8
MS.gene40326.t1	MTR_4g072840	51.724	87	12	4	1	87	1	57	2.36e-19	74.7
MS.gene40326.t1	MTR_5g021820	51.562	64	30	1	24	87	39	101	1.11e-18	74.3
MS.gene40326.t1	MTR_8g076040	51.562	64	30	1	24	87	40	102	1.40e-18	73.9
MS.gene40326.t1	MTR_2g043960	53.226	62	28	1	24	85	75	135	3.34e-18	74.3
MS.gene40326.t1	MTR_4g005310	46.053	76	40	1	11	85	48	123	4.25e-18	73.9
MS.gene40326.t1	MTR_1g094750	48.235	85	39	2	5	85	26	109	6.01e-18	72.8
MS.gene40326.t1	MTR_4g124750	51.613	62	29	1	24	85	68	128	2.63e-17	72.0
MS.gene40326.t1	MTR_1g009150	54.839	62	27	1	24	85	70	130	1.58e-16	70.5
MS.gene40326.t1	MTR_4g005320	52.459	61	28	1	25	85	70	129	7.49e-16	68.6
MS.gene40326.t1	MTR_0388s0020	44.286	70	35	2	11	77	61	129	1.11e-14	65.1
MS.gene40326.t1	MTR_3g105900	80.556	36	7	0	24	59	1171	1206	2.33e-14	67.0
MS.gene40326.t1	MTR_8g461210	43.023	86	41	3	4	82	7	91	3.53e-14	63.2
MS.gene40326.t1	MTR_3g109160	54.098	61	27	1	25	85	69	128	4.75e-14	63.9
MS.gene40326.t1	MTR_1g024085	47.761	67	34	1	16	82	28	93	1.04e-13	62.4
MS.gene40326.t1	MTR_7g118010	42.466	73	39	2	17	87	15	86	1.05e-13	61.6
MS.gene40326.t1	MTR_4g072160	47.059	68	32	2	24	88	36	102	2.70e-13	61.2
MS.gene40326.t1	MTR_3g097120	40.244	82	40	2	18	91	2	82	3.49e-13	60.1
MS.gene40326.t1	MTR_3g092220	43.478	69	36	2	16	82	24	91	7.28e-13	59.7
MS.gene40326.t1	MTR_3g098970	32.500	80	53	1	13	92	41	119	8.82e-13	60.1
MS.gene40326.t1	MTR_1g061570	48.387	62	31	1	24	85	18	78	1.16e-12	59.3
MS.gene40326.t1	MTR_1g063950	38.750	80	46	2	8	85	29	107	2.04e-12	58.5
MS.gene40326.t1	MTR_7g101740	45.312	64	34	1	24	87	39	101	2.72e-12	58.2
MS.gene40326.t1	MTR_8g026730	43.077	65	34	2	25	87	27	90	3.36e-12	57.8
MS.gene40326.t1	MTR_7g405740	45.902	61	32	1	25	85	67	126	5.58e-12	58.5
MS.gene40326.t1	MTR_8g101520	37.079	89	50	2	8	91	28	115	6.62e-12	58.2
MS.gene40326.t1	MTR_4g021810	43.038	79	41	3	5	82	8	83	9.05e-12	56.6
MS.gene40326.t1	MTR_8g096470	43.333	60	33	1	26	85	47	105	4.74e-11	54.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene40326.t1	AT4G38840	72.059	68	18	1	24	90	32	99	1.15e-34	114
MS.gene40326.t1	AT5G18020	63.158	76	27	1	16	90	15	90	1.59e-32	108
MS.gene40326.t1	AT5G18050	61.842	76	28	1	16	90	15	90	9.01e-32	107
MS.gene40326.t1	AT5G18060	61.842	76	28	1	16	90	15	90	2.24e-31	105
MS.gene40326.t1	AT5G18080	67.164	67	21	1	25	90	24	90	7.07e-31	104
MS.gene40326.t1	AT5G18030	65.672	67	22	1	25	90	22	88	1.44e-30	103
MS.gene40326.t1	AT2G21200	62.500	72	26	1	19	89	15	86	5.97e-30	102
MS.gene40326.t1	AT2G21210	56.410	78	32	1	15	90	18	95	1.20e-29	101
MS.gene40326.t1	AT4G38825	61.111	72	27	1	20	90	18	89	1.82e-29	101
MS.gene40326.t1	AT5G18010	59.211	76	30	1	16	90	15	90	1.87e-29	101
MS.gene40326.t1	AT4G34770	72.727	66	16	1	19	82	29	94	2.47e-29	101
MS.gene40326.t1	AT2G21210	56.410	78	32	1	15	90	85	162	9.06e-29	101
MS.gene40326.t1	AT3G03840	59.722	72	27	1	21	90	23	94	2.75e-27	95.9
MS.gene40326.t1	AT4G34800	53.488	86	33	2	1	83	1	82	4.43e-27	95.1
MS.gene40326.t1	AT4G34810	61.765	68	22	1	24	87	47	114	5.53e-27	95.9
MS.gene40326.t1	AT4G38850	55.422	83	25	2	9	88	14	87	7.67e-27	94.4
MS.gene40326.t1	AT3G03820	52.874	87	38	1	7	90	9	95	8.27e-27	94.7
MS.gene40326.t1	AT3G03850	62.687	67	23	1	26	90	26	92	1.85e-26	93.6
MS.gene40326.t1	AT3G03830	49.451	91	38	2	8	90	2	92	7.21e-26	92.0
MS.gene40326.t1	AT4G13790	50.000	82	34	2	9	88	14	90	1.05e-24	89.0
MS.gene40326.t1	AT4G34780	54.545	77	32	1	17	90	20	96	2.45e-24	88.6
MS.gene40326.t1	AT4G34790	63.492	63	21	1	25	85	39	101	7.96e-24	87.4
MS.gene40326.t1	AT4G38860	56.923	65	28	0	23	87	38	102	5.59e-23	85.1
MS.gene40326.t1	AT1G75580	52.703	74	34	1	14	87	33	105	4.23e-22	83.2
MS.gene40326.t1	AT4G34760	55.385	65	28	1	23	87	41	104	4.95e-21	80.1
MS.gene40326.t1	AT2G21220	52.308	65	30	1	23	87	38	101	1.83e-20	78.6
MS.gene40326.t1	AT4G36110	51.515	66	31	1	24	89	39	103	1.83e-19	76.3
MS.gene40326.t1	AT3G20220	54.545	66	28	2	25	89	47	111	2.59e-19	76.3
MS.gene40326.t1	AT2G16580	52.308	65	30	1	23	87	42	105	4.30e-19	75.5
MS.gene40326.t1	AT2G18010	50.000	66	32	1	24	89	47	111	1.21e-18	74.3
MS.gene40326.t1	AT3G43120	51.613	62	29	1	24	85	78	138	1.72e-18	75.1
MS.gene40326.t1	AT1G19830	58.333	60	24	1	23	82	46	104	2.48e-18	73.6
MS.gene40326.t1	AT5G20810	53.226	62	28	1	24	85	78	138	1.30e-17	73.2
MS.gene40326.t1	AT5G20810	53.226	62	28	1	24	85	78	138	3.11e-17	72.8
MS.gene40326.t1	AT5G66260	42.391	92	49	2	2	90	8	98	1.56e-15	66.2
MS.gene40326.t1	AT5G10990	47.222	72	37	1	11	82	27	97	2.59e-15	66.6
MS.gene40326.t1	AT1G75590	47.619	84	38	3	4	82	16	98	3.41e-15	66.6
MS.gene40326.t1	AT4G34750	41.026	78	45	1	10	87	22	98	3.65e-15	66.2
MS.gene40326.t1	AT4G34750	41.026	78	45	1	10	87	22	98	3.65e-15	66.2
MS.gene40326.t1	AT1G19840	45.783	83	40	2	4	82	16	97	2.12e-14	64.7
MS.gene40326.t1	AT3G51200	50.820	61	28	2	24	83	38	97	4.32e-14	62.4
MS.gene40326.t1	AT2G46690	41.892	74	39	2	17	87	16	88	5.32e-14	62.8
MS.gene40326.t1	AT2G24400	50.000	66	32	1	20	85	59	123	8.69e-14	63.5
MS.gene40326.t1	AT4G31320	50.820	61	29	1	25	85	80	139	9.87e-14	63.5
MS.gene40326.t1	AT2G37030	39.744	78	44	2	11	86	33	109	1.75e-13	61.6
MS.gene40326.t1	AT3G61900	38.095	84	48	2	10	90	15	97	3.59e-13	60.8
MS.gene40326.t1	AT3G12830	40.000	75	44	1	11	85	36	109	1.23e-12	59.3
MS.gene40326.t1	AT5G53590	39.726	73	38	2	24	91	45	116	5.60e-12	58.2
MS.gene40326.t1	AT3G53250	42.857	63	32	1	24	86	51	109	7.92e-12	57.4
MS.gene40326.t1	AT4G00880	46.970	66	32	2	24	87	27	91	1.32e-11	56.6
MS.gene40326.t1	AT3G53250	42.857	63	32	1	24	86	82	140	1.56e-11	57.0
MS.gene40326.t1	AT1G16510	42.029	69	39	1	14	82	43	110	2.95e-11	56.2

Find 19 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTCTAATAGCTGCAGGTAAA+CGG	0.409491	3.4:-94898568	None:intergenic
AATCAAGTGGAGGAAGAATT+CGG	0.436017	3.4:+94898731	MS.gene40326:CDS
TCAAAACTTGTTGAATCAAG+TGG	0.492846	3.4:+94898718	MS.gene40326:CDS
GAAGCTCGTCTAATAGCTGC+AGG	0.514149	3.4:-94898575	None:intergenic
TTCGGATATGATCATCCAAT+GGG	0.523692	3.4:+94898749	MS.gene40326:CDS
AAAAGTATTGAATGTGCCAA+AGG	0.534600	3.4:+94898619	MS.gene40326:CDS
GGAATTGTGAGGCCACCCAT+TGG	0.542714	3.4:-94898764	None:intergenic
TACACTGCAAGATAACCCTT+TGG	0.544929	3.4:-94898635	None:intergenic
AAAGTATTGAATGTGCCAAA+GGG	0.556385	3.4:+94898620	MS.gene40326:CDS
GGTTATCTTGCAGTGTACGT+TGG	0.557125	3.4:+94898641	MS.gene40326:CDS
ATTCGGATATGATCATCCAA+TGG	0.569256	3.4:+94898748	MS.gene40326:CDS
AAGAAAACATCTTCTGTGCA+AGG	0.580973	3.4:-94898785	None:intergenic
AGCTTGGTTCAAGTATGACA+TGG	0.610689	3.4:-94898690	None:intergenic
AAACTTGTTGAATCAAGTGG+AGG	0.613536	3.4:+94898721	MS.gene40326:CDS
GCTTGGTTCAAGTATGACAT+GGG	0.618074	3.4:-94898689	None:intergenic
GGATATGATCATCCAATGGG+TGG	0.663522	3.4:+94898752	MS.gene40326:CDS
CTTGGTTCAAGTATGACATG+GGG	0.689184	3.4:-94898688	None:intergenic
ACGTTGGAGAGCAAATGAAG+CGG	0.725765	3.4:+94898657	MS.gene40326:CDS
CTTCTGTGCAAGGAATTGTG+AGG	0.738315	3.4:-94898775	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CATTCAATACTTTTGATGTT+TGG	-	chr3.4:94898613-94898632	None:intergenic	25.0%
!!	AAAAGTATTGAATGTGCCAA+AGG	+	chr3.4:94898619-94898638	MS.gene40326:CDS	30.0%
!!	AAAGTATTGAATGTGCCAAA+GGG	+	chr3.4:94898620-94898639	MS.gene40326:CDS	30.0%
!!	TCAAAACTTGTTGAATCAAG+TGG	+	chr3.4:94898718-94898737	MS.gene40326:CDS	30.0%
!!!	ACAAGTTTTGAAATGAAGCT+TGG	-	chr3.4:94898709-94898728	None:intergenic	30.0%
	AAGAAAACATCTTCTGTGCA+AGG	-	chr3.4:94898788-94898807	None:intergenic	35.0%
	AATCAAGTGGAGGAAGAATT+CGG	+	chr3.4:94898731-94898750	MS.gene40326:CDS	35.0%
	ATTCGGATATGATCATCCAA+TGG	+	chr3.4:94898748-94898767	MS.gene40326:CDS	35.0%
	TTCGGATATGATCATCCAAT+GGG	+	chr3.4:94898749-94898768	MS.gene40326:CDS	35.0%
!!	AAACTTGTTGAATCAAGTGG+AGG	+	chr3.4:94898721-94898740	MS.gene40326:CDS	35.0%
	AGCTTGGTTCAAGTATGACA+TGG	-	chr3.4:94898693-94898712	None:intergenic	40.0%
	CTTGGTTCAAGTATGACATG+GGG	-	chr3.4:94898691-94898710	None:intergenic	40.0%
	GCTTGGTTCAAGTATGACAT+GGG	-	chr3.4:94898692-94898711	None:intergenic	40.0%
	GTCTAATAGCTGCAGGTAAA+CGG	-	chr3.4:94898571-94898590	None:intergenic	40.0%
	TACACTGCAAGATAACCCTT+TGG	-	chr3.4:94898638-94898657	None:intergenic	40.0%
	ACGTTGGAGAGCAAATGAAG+CGG	+	chr3.4:94898657-94898676	MS.gene40326:CDS	45.0%
	CTTCTGTGCAAGGAATTGTG+AGG	-	chr3.4:94898778-94898797	None:intergenic	45.0%
	GGATATGATCATCCAATGGG+TGG	+	chr3.4:94898752-94898771	MS.gene40326:CDS	45.0%
	GGTTATCTTGCAGTGTACGT+TGG	+	chr3.4:94898641-94898660	MS.gene40326:CDS	45.0%
	GAAGCTCGTCTAATAGCTGC+AGG	-	chr3.4:94898578-94898597	None:intergenic	50.0%
	GGAATTGTGAGGCCACCCAT+TGG	-	chr3.4:94898767-94898786	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	94898560	94898838	94898560	ID=MS.gene40326
chr3.4	mRNA	94898560	94898838	94898560	ID=MS.gene40326.t1;Parent=MS.gene40326
chr3.4	exon	94898560	94898838	94898560	ID=MS.gene40326.t1.exon1;Parent=MS.gene40326.t1
chr3.4	CDS	94898560	94898838	94898560	ID=cds.MS.gene40326.t1;Parent=MS.gene40326.t1

Gene Sequence	>MS.gene40326 ATGGGTTTCCGTTTACCTGCAGCTATTAGACGAGCTTCATTTAGCAGTAGCCAAACATCAAAAGTATTGAATGTGCCAAAGGGTTATCTTGCAGTGTACGTTGGAGAGCAAATGAAGCGGTTTGTTATCCCCATGTCATACTTGAACCAAGCTTCATTTCAAAACTTGTTGAATCAAGTGGAGGAAGAATTCGGATATGATCATCCAATGGGTGGCCTCACAATTCCTTGCACAGAAGATGTTTTCTTGCATATTACTGCTCGCTTCAATGAGCTATAA
Protein sequence	>MS.gene40326.t1 MGFRLPAAIRRASFSSSQTSKVLNVPKGYLAVYVGEQMKRFVIPMSYLNQASFQNLLNQVEEEFGYDHPMGGLTIPCTEDVFLHITARFNEL