AlfalfaGEDB | MS.gene047517

M. sativa cultivar XinJiangDaYe / MS.gene047517

KEGG: K14488
GO: GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
PFAM: PF02519.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene047517.t1	AES63709.1	91.6	83	7	0	1	83	1	83	2.10E-34	154.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene047517.t1	P33083	68.7	83	25	1	1	83	1	82	4.4e-25	115.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene047517.t1	G7ILE9	91.6	83	7	0	1	83	1	83	1.5e-34	154.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene047517	MS.gene50669	PPI
MS.gene003626	MS.gene047517	PPI
MS.gene047517	MS.gene70685	PPI
MS.gene047517	MS.gene003622	PPI
MS.gene062946	MS.gene047517	PPI
MS.gene047517	MS.gene003599	PPI
MS.gene057805	MS.gene047517	PPI
MS.gene047517	MS.gene23368	PPI
MS.gene003605	MS.gene047517	PPI
MS.gene047517	MS.gene50662	PPI
MS.gene047517	MS.gene003585	PPI
MS.gene047517	MS.gene50660	PPI
MS.gene047517	MS.gene003623	PPI
MS.gene047517	MS.gene50668	PPI
MS.gene047517	MS.gene003581	PPI
MS.gene062971	MS.gene047517	PPI
MS.gene23373	MS.gene047517	PPI
MS.gene047517	MS.gene062958	PPI
MS.gene047517	MS.gene003628	PPI
MS.gene047517	MS.gene057798	PPI
MS.gene047517	MS.gene057794	PPI
MS.gene047517	MS.gene003629	PPI
MS.gene047517	MS.gene062983	PPI
MS.gene047517	MS.gene062966	PPI
MS.gene047517	MS.gene23367	PPI
MS.gene062975	MS.gene047517	PPI
MS.gene047517	MS.gene062940	PPI
MS.gene50689	MS.gene047517	PPI
MS.gene047517	MS.gene003595	PPI
MS.gene047517	MS.gene062959	PPI
MS.gene003576	MS.gene047517	PPI
MS.gene047517	MS.gene50664	PPI
MS.gene047517	MS.gene057785	PPI
MS.gene047517	MS.gene50773	PPI
MS.gene047517	MS.gene50670	PPI
MS.gene047517	MS.gene057800	PPI
MS.gene047517	MS.gene50653	PPI
MS.gene047517	MS.gene003598	PPI
MS.gene047517	MS.gene50690	PPI
MS.gene062970	MS.gene047517	PPI
MS.gene047517	MS.gene062941	PPI
MS.gene062942	MS.gene047517	PPI
MS.gene047517	MS.gene003613	PPI
MS.gene047517	MS.gene057799	PPI
MS.gene047517	MS.gene50688	PPI
MS.gene047517	MS.gene003603	PPI
MS.gene047517	MS.gene003630	PPI
MS.gene062972	MS.gene047517	PPI
MS.gene047517	MS.gene057779	PPI
MS.gene047517	MS.gene062980	PPI
MS.gene047517	MS.gene50673	PPI
MS.gene047517	MS.gene003610	PPI
MS.gene003582	MS.gene047517	PPI
MS.gene047517	MS.gene50771	PPI
MS.gene047517	MS.gene022358	PPI
MS.gene047517	MS.gene057802	PPI
MS.gene047517	MS.gene057796	PPI
MS.gene047517	MS.gene062954	PPI
MS.gene047517	MS.gene50682	PPI
MS.gene50772	MS.gene047517	PPI
MS.gene047517	MS.gene003597	PPI
MS.gene057809	MS.gene047517	PPI
MS.gene047517	MS.gene057806	PPI
MS.gene047517	MS.gene057780	PPI
MS.gene047517	MS.gene057788	PPI
MS.gene062978	MS.gene047517	PPI
MS.gene047517	MS.gene062944	PPI
MS.gene047517	MS.gene50658	PPI
MS.gene047517	MS.gene50655	PPI
MS.gene047517	MS.gene28863	PPI
MS.gene047517	MS.gene50685	PPI
MS.gene047517	MS.gene003584	PPI
MS.gene047517	MS.gene057791	PPI
MS.gene047517	MS.gene50674	PPI
MS.gene003593	MS.gene047517	PPI
MS.gene047517	MS.gene50651	PPI
MS.gene047517	MS.gene057782	PPI
MS.gene047517	MS.gene062969	PPI
MS.gene003580	MS.gene047517	PPI
MS.gene047517	MS.gene062965	PPI
MS.gene047517	MS.gene50666	PPI
MS.gene047517	MS.gene057784	PPI
MS.gene047517	MS.gene062953	PPI
MS.gene057816	MS.gene047517	PPI
MS.gene047517	MS.gene23369	PPI
MS.gene047517	MS.gene062986	PPI
MS.gene047517	MS.gene057783	PPI
MS.gene047517	MS.gene50770	PPI
MS.gene047517	MS.gene062987	PPI
MS.gene047517	MS.gene057811	PPI
MS.gene047517	MS.gene057817	PPI
MS.gene047517	MS.gene70684	PPI
MS.gene047517	MS.gene062964	PPI
MS.gene047517	MS.gene003612	PPI
MS.gene047517	MS.gene77525	PPI
MS.gene047517	MS.gene057777	PPI
MS.gene50657	MS.gene047517	PPI
MS.gene047517	MS.gene062945	PPI
MS.gene047517	MS.gene062967	PPI
MS.gene047517	MS.gene057804	PPI
MS.gene047517	MS.gene003579	PPI
MS.gene047517	MS.gene062989	PPI
MS.gene50683	MS.gene047517	PPI
MS.gene047517	MS.gene003607	PPI
MS.gene057807	MS.gene047517	PPI
MS.gene047517	MS.gene50656	PPI
MS.gene047517	MS.gene003606	PPI
MS.gene047517	MS.gene50686	PPI
MS.gene047517	MS.gene70683	PPI
MS.gene047517	MS.gene062962	PPI
MS.gene047517	MS.gene062985	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene047517.t1	MTR_4g050700	91.566	83	7	0	1	83	1	83	2.40e-52	159
MS.gene047517.t1	MTR_2g012410	91.566	83	7	0	1	83	1	83	2.40e-52	159
MS.gene047517.t1	MTR_3g084170	85.542	83	11	1	1	83	1	82	4.11e-47	146
MS.gene047517.t1	MTR_3g084240	84.337	83	12	1	1	83	1	82	4.85e-46	143
MS.gene047517.t1	MTR_3g110038	79.070	86	17	1	1	86	1	85	1.59e-44	139
MS.gene047517.t1	MTR_3g084260	80.723	83	16	0	1	83	1	83	2.86e-44	139
MS.gene047517.t1	MTR_3g110032	79.518	83	17	0	1	83	1	83	6.59e-44	138
MS.gene047517.t1	MTR_3g084150	78.313	83	17	1	1	83	1	82	1.12e-43	137
MS.gene047517.t1	MTR_3g084220	80.723	83	15	1	1	83	1	82	1.42e-43	137
MS.gene047517.t1	MTR_8g461410	77.108	83	18	1	1	83	1	82	2.49e-43	136
MS.gene047517.t1	MTR_3g084180	77.108	83	18	1	1	83	1	82	9.29e-43	135
MS.gene047517.t1	MTR_3g110042	77.108	83	18	1	1	83	1	82	3.01e-42	134
MS.gene047517.t1	MTR_4g072870	69.767	86	26	0	1	86	1	86	2.01e-39	126
MS.gene047517.t1	MTR_3g117630	76.250	80	19	0	4	83	3	82	7.88e-39	125
MS.gene047517.t1	MTR_4g072310	69.880	83	25	0	1	83	1	83	8.95e-39	125
MS.gene047517.t1	MTR_4g072490	71.084	83	18	1	1	83	1	77	9.74e-39	124
MS.gene047517.t1	MTR_4g072240	69.767	86	26	0	1	86	1	86	1.49e-38	124
MS.gene047517.t1	MTR_4g072880	68.675	83	26	0	1	83	1	83	1.60e-38	124
MS.gene047517.t1	MTR_4g072250	71.084	83	23	1	1	83	1	82	3.07e-38	123
MS.gene047517.t1	MTR_4g072470	72.289	83	17	1	1	83	1	77	6.56e-38	122
MS.gene047517.t1	MTR_4g072370	71.084	83	21	1	1	83	1	80	7.67e-38	122
MS.gene047517.t1	MTR_4g072330	69.880	83	22	1	1	83	1	80	9.76e-38	122
MS.gene047517.t1	MTR_4g072740	71.084	83	18	1	1	83	1	77	1.12e-37	122
MS.gene047517.t1	MTR_4g072270	67.470	83	27	0	1	83	1	83	1.22e-37	122
MS.gene047517.t1	MTR_3g084200	79.452	73	14	1	1	73	1	72	1.74e-37	122
MS.gene047517.t1	MTR_4g072300	69.880	83	25	0	1	83	1	83	1.98e-37	121
MS.gene047517.t1	MTR_4g072830	71.084	83	21	1	1	83	1	80	2.30e-37	121
MS.gene047517.t1	MTR_4g072860	68.675	83	26	0	1	83	1	83	5.70e-37	120
MS.gene047517.t1	MTR_4g072220	67.470	83	27	0	1	83	1	83	2.17e-36	119
MS.gene047517.t1	MTR_4g072640	68.675	83	25	1	1	83	1	82	3.49e-36	118
MS.gene047517.t1	MTR_4g072500	67.470	83	26	1	1	83	1	82	3.98e-36	118
MS.gene047517.t1	MTR_4g072260	65.116	86	30	0	1	86	1	86	6.08e-36	117
MS.gene047517.t1	MTR_4g072230	68.675	83	24	1	1	83	1	81	8.61e-36	117
MS.gene047517.t1	MTR_4g072630	67.470	83	22	1	1	83	1	78	9.79e-36	117
MS.gene047517.t1	MTR_4g072580	67.470	83	21	1	1	83	1	77	1.06e-35	117
MS.gene047517.t1	MTR_4g072570	67.470	83	26	1	1	83	1	82	1.19e-35	117
MS.gene047517.t1	MTR_4g072530	67.470	83	26	1	1	83	1	82	1.41e-35	117
MS.gene047517.t1	MTR_4g072890	65.116	86	30	0	1	86	1	86	2.81e-35	116
MS.gene047517.t1	MTR_3g084250	66.265	83	27	1	1	83	1	82	3.16e-35	116
MS.gene047517.t1	MTR_4g072480	65.060	83	28	1	1	83	1	82	6.30e-35	115
MS.gene047517.t1	MTR_4g072680	66.265	83	27	1	1	83	1	82	1.23e-34	114
MS.gene047517.t1	MTR_4g072620	63.855	83	29	1	1	83	1	82	3.22e-34	113
MS.gene047517.t1	MTR_4g072465	65.060	83	24	1	1	83	1	78	3.49e-34	113
MS.gene047517.t1	MTR_4g072730	63.855	83	29	1	1	83	1	82	5.95e-34	112
MS.gene047517.t1	MTR_5g008350	67.500	80	20	1	1	80	1	74	6.93e-34	112
MS.gene047517.t1	MTR_4g072850	67.857	84	26	1	1	83	1	84	9.67e-34	112
MS.gene047517.t1	MTR_4g072510	67.470	83	22	1	1	83	1	78	1.80e-33	111
MS.gene047517.t1	MTR_4g072380	67.470	83	22	1	1	83	1	78	2.33e-33	111
MS.gene047517.t1	MTR_4g072460	62.069	87	32	1	1	87	1	86	2.50e-33	111
MS.gene047517.t1	MTR_4g072610	68.675	83	19	2	1	83	1	76	2.77e-33	110
MS.gene047517.t1	MTR_4g072720	68.675	83	21	1	1	83	1	78	3.52e-33	110
MS.gene047517.t1	MTR_4g072540	66.265	83	23	1	1	83	1	78	4.33e-33	110
MS.gene047517.t1	MTR_4g072670	67.470	83	22	1	1	83	1	78	8.55e-33	109
MS.gene047517.t1	MTR_4g072600	63.855	83	24	1	1	83	1	77	1.36e-32	109
MS.gene047517.t1	MTR_4g072590	66.265	83	22	1	1	83	1	77	3.73e-32	108
MS.gene047517.t1	MTR_4g072650	66.265	83	21	2	1	83	1	76	9.21e-32	107
MS.gene047517.t1	MTR_3g084210	67.470	83	26	1	1	83	1	82	2.90e-31	106
MS.gene047517.t1	MTR_4g072820	63.953	86	25	2	1	86	1	80	2.97e-31	105
MS.gene047517.t1	MTR_3g084190	67.470	83	26	1	1	83	1	82	1.23e-30	104
MS.gene047517.t1	MTR_5g008370	64.706	85	25	2	1	83	1	82	1.84e-30	105
MS.gene047517.t1	MTR_4g072450	65.060	83	23	1	1	83	1	77	2.53e-30	103
MS.gene047517.t1	MTR_8g461420	66.265	83	27	1	1	83	1	82	3.05e-30	103
MS.gene047517.t1	MTR_3g084160	66.265	83	27	1	1	83	1	82	4.19e-30	103
MS.gene047517.t1	MTR_4g072690	62.651	83	25	1	1	83	1	77	4.47e-30	102
MS.gene047517.t1	MTR_3g084230	66.265	83	27	1	1	83	1	82	7.24e-30	102
MS.gene047517.t1	MTR_5g008360	68.116	69	22	0	15	83	5	73	8.42e-30	102
MS.gene047517.t1	MTR_4g072940	64.634	82	28	1	3	83	33	114	2.31e-29	102
MS.gene047517.t1	MTR_4g072660	59.036	83	28	1	1	83	1	77	1.17e-28	99.4
MS.gene047517.t1	MTR_3g110045	67.901	81	25	1	3	83	62	141	5.23e-28	99.8
MS.gene047517.t1	MTR_4g072550	56.977	86	35	1	4	87	12	97	1.73e-27	97.1
MS.gene047517.t1	MTR_4g072980	56.180	89	35	3	1	86	1	88	3.17e-27	95.9
MS.gene047517.t1	MTR_4g072770	67.164	67	21	1	26	92	15	80	8.70e-27	94.4
MS.gene047517.t1	MTR_4g072560	50.549	91	38	2	1	84	1	91	9.17e-27	95.1
MS.gene047517.t1	MTR_4g072910	59.459	74	29	1	20	93	29	101	1.63e-25	91.7
MS.gene047517.t1	MTR_4g072280	58.667	75	31	0	1	75	1	75	1.48e-24	88.6
MS.gene047517.t1	MTR_3g117640	50.526	95	38	2	1	86	1	95	2.04e-24	89.0
MS.gene047517.t1	MTR_4g072750	60.811	74	26	2	1	72	1	73	2.94e-23	85.5
MS.gene047517.t1	MTR_8g461400	56.627	83	9	2	1	83	1	56	2.17e-22	82.8
MS.gene047517.t1	MTR_4g072290	50.602	83	27	1	1	83	1	69	5.79e-21	79.7
MS.gene047517.t1	MTR_3g110035	64.286	56	20	0	28	83	73	128	1.38e-20	80.5
MS.gene047517.t1	MTR_2g043960	51.389	72	34	1	16	87	66	136	8.22e-18	73.9
MS.gene047517.t1	MTR_4g072930	48.684	76	34	2	16	86	30	105	8.33e-18	72.4
MS.gene047517.t1	MTR_3g113310	52.542	59	27	1	25	83	41	98	8.51e-18	72.4
MS.gene047517.t1	MTR_6g077850	46.377	69	36	1	24	92	32	99	1.71e-17	71.2
MS.gene047517.t1	MTR_3g117610	55.932	59	25	1	25	83	44	101	1.85e-17	71.6
MS.gene047517.t1	MTR_4g124750	53.968	63	28	1	25	87	68	129	5.28e-17	71.6
MS.gene047517.t1	MTR_3g105900	51.948	77	37	0	23	99	1169	1245	5.58e-17	75.1
MS.gene047517.t1	MTR_4g072190	49.275	69	34	1	15	83	25	92	1.03e-16	69.3
MS.gene047517.t1	MTR_1g009150	48.000	75	38	1	19	93	64	137	4.52e-16	69.7
MS.gene047517.t1	MTR_8g076040	51.613	62	29	1	25	86	40	100	8.13e-16	67.4
MS.gene047517.t1	MTR_5g021820	52.542	59	27	1	25	83	39	96	1.59e-15	66.6
MS.gene047517.t1	MTR_4g005320	45.455	77	39	2	26	102	70	143	1.78e-14	65.5
MS.gene047517.t1	MTR_4g072840	47.674	86	14	4	1	86	1	55	3.22e-14	62.0
MS.gene047517.t1	MTR_1g094750	42.529	87	45	3	11	93	31	116	6.83e-14	62.8
MS.gene047517.t1	MTR_4g005310	47.059	68	35	1	22	88	58	125	1.64e-13	62.4
MS.gene047517.t1	MTR_3g109160	45.570	79	40	2	11	87	52	129	8.88e-13	60.8
MS.gene047517.t1	MTR_3g092220	40.260	77	41	3	9	83	18	91	2.25e-11	56.2
MS.gene047517.t1	MTR_8g461210	49.153	59	29	1	25	83	34	91	4.93e-11	55.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene047517.t1	AT4G38840	56.383	94	33	2	1	86	1	94	1.01e-27	97.4
MS.gene047517.t1	AT4G34800	58.974	78	29	1	13	87	8	85	1.09e-27	97.1
MS.gene047517.t1	AT5G18060	64.706	68	23	1	17	83	15	82	5.32e-27	95.1
MS.gene047517.t1	AT4G38825	65.672	67	22	1	18	83	15	81	5.46e-27	95.1
MS.gene047517.t1	AT2G21200	66.154	65	21	1	20	83	15	79	6.68e-27	95.1
MS.gene047517.t1	AT5G18050	63.235	68	24	1	17	83	15	82	7.89e-27	94.7
MS.gene047517.t1	AT5G18020	63.235	68	24	1	17	83	15	82	1.10e-26	94.4
MS.gene047517.t1	AT5G18080	63.235	68	24	1	17	83	15	82	2.78e-26	93.6
MS.gene047517.t1	AT4G34770	61.039	77	28	1	12	86	21	97	6.56e-26	92.8
MS.gene047517.t1	AT5G18030	55.696	79	32	2	8	83	2	80	2.82e-25	90.9
MS.gene047517.t1	AT2G21210	60.563	71	25	2	15	83	18	87	6.01e-25	90.5
MS.gene047517.t1	AT5G18010	60.294	68	26	1	17	83	15	82	3.26e-24	88.2
MS.gene047517.t1	AT2G21210	60.563	71	25	2	15	83	85	154	5.11e-24	90.1
MS.gene047517.t1	AT4G34810	56.944	72	27	1	25	92	47	118	5.34e-24	88.6
MS.gene047517.t1	AT4G38850	63.492	63	20	1	24	83	19	81	1.19e-23	86.7
MS.gene047517.t1	AT3G03840	51.765	85	32	2	8	83	2	86	2.80e-22	83.6
MS.gene047517.t1	AT3G03850	52.941	85	29	3	8	83	2	84	8.90e-22	82.0
MS.gene047517.t1	AT3G03820	54.167	72	30	1	15	83	16	87	9.55e-22	82.0
MS.gene047517.t1	AT3G03830	49.398	83	35	2	8	83	2	84	1.06e-21	82.0
MS.gene047517.t1	AT4G34790	57.812	64	25	1	26	87	39	102	8.18e-20	77.8
MS.gene047517.t1	AT4G13790	46.753	77	34	2	9	83	13	84	1.92e-19	76.3
MS.gene047517.t1	AT3G43120	47.945	73	37	1	15	87	68	139	2.44e-18	75.1
MS.gene047517.t1	AT1G75580	57.627	59	24	1	25	83	43	100	8.79e-18	72.4
MS.gene047517.t1	AT4G34780	56.250	64	25	1	23	83	25	88	1.06e-17	72.0
MS.gene047517.t1	AT5G20810	45.455	88	43	3	15	102	68	150	1.61e-17	73.2
MS.gene047517.t1	AT4G38860	52.239	67	30	1	25	91	39	103	1.67e-17	71.6
MS.gene047517.t1	AT5G20810	45.977	87	42	3	16	102	69	150	2.31e-17	73.6
MS.gene047517.t1	AT4G34760	55.932	59	25	1	25	83	42	99	2.54e-17	71.2
MS.gene047517.t1	AT4G36110	47.761	67	34	1	17	83	31	96	4.24e-16	68.2
MS.gene047517.t1	AT2G16580	52.542	59	27	1	25	83	43	100	8.31e-16	67.4
MS.gene047517.t1	AT2G21220	50.000	60	29	1	24	83	38	96	1.21e-15	67.0
MS.gene047517.t1	AT3G20220	46.154	78	40	2	15	91	36	112	1.94e-15	66.6
MS.gene047517.t1	AT2G18010	49.206	63	31	1	25	87	47	108	5.54e-15	65.5
MS.gene047517.t1	AT1G19830	50.794	63	30	1	25	87	47	108	6.80e-15	65.5
MS.gene047517.t1	AT4G31320	47.222	72	37	1	26	97	80	150	8.49e-13	61.6
MS.gene047517.t1	AT2G24400	50.769	65	31	1	23	87	61	124	2.91e-12	59.7
MS.gene047517.t1	AT3G51200	43.750	80	43	2	15	93	28	106	3.13e-12	58.2
MS.gene047517.t1	AT5G66260	49.206	63	31	1	25	87	33	94	3.97e-12	57.8
MS.gene047517.t1	AT1G75590	42.466	73	41	1	11	83	27	98	7.21e-12	58.5
MS.gene047517.t1	AT5G10990	44.118	68	37	1	16	83	31	97	3.47e-11	56.6

Find 24 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATGTTACTTCTAGCTTCAA+TGG	0.317755	2.1:-72065673	MS.gene047517:CDS
GCAAGTATTGAAATGAAGTT+TGG	0.325729	2.1:+72065775	None:intergenic
TTGTTATAGCTGCAGGTATA+CGG	0.359507	2.1:+72065916	None:intergenic
TTGCTACTTTCGATGACGTT+TGG	0.389337	2.1:+72065874	None:intergenic
GTCGCCCTTGTTATAGCTGC+AGG	0.483277	2.1:+72065909	None:intergenic
TGTAAGTCTCTCGCAGCTGC+AGG	0.487344	2.1:+72065642	None:intergenic
TTCCGATACGATCATCCAAT+GGG	0.534809	2.1:-72065732	MS.gene047517:CDS
GGAATTGTGAGGCCACCCAT+TGG	0.551960	2.1:+72065717	None:intergenic
GGATATCTTGCGGTGTACGT+TGG	0.555155	2.1:-72065840	MS.gene047517:CDS
GTATACCTGCAGCTATAACA+AGG	0.565691	2.1:-72065914	MS.gene047517:CDS
TACACCGCAAGATATCCCTT+TGG	0.570332	2.1:+72065846	None:intergenic
AAAGTAGCAAATGTGCCAAA+GGG	0.571373	2.1:-72065861	MS.gene047517:CDS
ATTCCGATACGATCATCCAA+TGG	0.589813	2.1:-72065733	MS.gene047517:CDS
CACCCATTGGATGATCGTAT+CGG	0.591752	2.1:+72065730	None:intergenic
GAAAGTAGCAAATGTGCCAA+AGG	0.593992	2.1:-72065862	MS.gene047517:CDS
GTTTGGTTCAAGTATGACGT+AGG	0.594838	2.1:+72065792	None:intergenic
TTTGGTTCAAGTATGACGTA+GGG	0.596423	2.1:+72065793	None:intergenic
ATACTTGCTGAGTCGTTCAG+AGG	0.600415	2.1:-72065760	MS.gene047517:CDS
TATACCTGCAGCTATAACAA+GGG	0.634825	2.1:-72065913	MS.gene047517:CDS
TCTTGCGGTGTACGTTGGAG+AGG	0.635448	2.1:-72065835	MS.gene047517:CDS
CGATACGATCATCCAATGGG+TGG	0.653344	2.1:-72065729	MS.gene047517:CDS
TGTGCCAAAGGGATATCTTG+CGG	0.661569	2.1:-72065850	MS.gene047517:CDS
CTTCTGTGCTAGGAATTGTG+AGG	0.712168	2.1:+72065706	None:intergenic
ACGTTGGAGAGGAAATGAAG+CGG	0.725765	2.1:-72065824	MS.gene047517:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GCAAGTATTGAAATGAAGTT+TGG	+	chr2.1:72065790-72065809	None:intergenic	30.0%
	AAAGTAGCAAATGTGCCAAA+GGG	-	chr2.1:72065701-72065720	MS.gene047517:CDS	35.0%
	CAAAAAACATCTTCTGTGCT+AGG	+	chr2.1:72065869-72065888	None:intergenic	35.0%
	TATACCTGCAGCTATAACAA+GGG	-	chr2.1:72065649-72065668	MS.gene047517:CDS	35.0%
	TTGTTATAGCTGCAGGTATA+CGG	+	chr2.1:72065649-72065668	None:intergenic	35.0%
!	TTTGGTTCAAGTATGACGTA+GGG	+	chr2.1:72065772-72065791	None:intergenic	35.0%
!!	CATGTTACTTCTAGCTTCAA+TGG	-	chr2.1:72065889-72065908	MS.gene047517:CDS	35.0%
	ATTCCGATACGATCATCCAA+TGG	-	chr2.1:72065829-72065848	MS.gene047517:CDS	40.0%
	GAAAGTAGCAAATGTGCCAA+AGG	-	chr2.1:72065700-72065719	MS.gene047517:CDS	40.0%
	GTATACCTGCAGCTATAACA+AGG	-	chr2.1:72065648-72065667	MS.gene047517:CDS	40.0%
	TTCCGATACGATCATCCAAT+GGG	-	chr2.1:72065830-72065849	MS.gene047517:CDS	40.0%
!	GTTTGGTTCAAGTATGACGT+AGG	+	chr2.1:72065773-72065792	None:intergenic	40.0%
!!	TTGCTACTTTCGATGACGTT+TGG	+	chr2.1:72065691-72065710	None:intergenic	40.0%
	ACGTTGGAGAGGAAATGAAG+CGG	-	chr2.1:72065738-72065757	MS.gene047517:CDS	45.0%
	CACCCATTGGATGATCGTAT+CGG	+	chr2.1:72065835-72065854	None:intergenic	45.0%
	CTTCTGTGCTAGGAATTGTG+AGG	+	chr2.1:72065859-72065878	None:intergenic	45.0%
	TACACCGCAAGATATCCCTT+TGG	+	chr2.1:72065719-72065738	None:intergenic	45.0%
	TGTGCCAAAGGGATATCTTG+CGG	-	chr2.1:72065712-72065731	MS.gene047517:CDS	45.0%
!	ATACTTGCTGAGTCGTTCAG+AGG	-	chr2.1:72065802-72065821	MS.gene047517:CDS	45.0%
	CGATACGATCATCCAATGGG+TGG	-	chr2.1:72065833-72065852	MS.gene047517:CDS	50.0%
	GGATATCTTGCGGTGTACGT+TGG	-	chr2.1:72065722-72065741	MS.gene047517:CDS	50.0%
	GGAATTGTGAGGCCACCCAT+TGG	+	chr2.1:72065848-72065867	None:intergenic	55.0%
	TCTTGCGGTGTACGTTGGAG+AGG	-	chr2.1:72065727-72065746	MS.gene047517:CDS	55.0%
	TGTAAGTCTCTCGCAGCTGC+AGG	+	chr2.1:72065923-72065942	None:intergenic	55.0%
!	GTCGCCCTTGTTATAGCTGC+AGG	+	chr2.1:72065656-72065675	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	72065638	72065946	72065638	ID=MS.gene047517
chr2.1	mRNA	72065638	72065946	72065638	ID=MS.gene047517.t1;Parent=MS.gene047517
chr2.1	exon	72065638	72065946	72065638	ID=MS.gene047517.t1.exon1;Parent=MS.gene047517.t1
chr2.1	CDS	72065638	72065946	72065638	ID=cds.MS.gene047517.t1;Parent=MS.gene047517.t1

Gene Sequence	>MS.gene047517 ATGGGCTTCCGTATACCTGCAGCTATAACAAGGGCGACATTTAGCAGCAGCCAAACGTCATCGAAAGTAGCAAATGTGCCAAAGGGATATCTTGCGGTGTACGTTGGAGAGGAAATGAAGCGGTTTGTGATCCCTACGTCATACTTGAACCAAACTTCATTTCAATACTTGCTGAGTCGTTCAGAGGAAGAATTCCGATACGATCATCCAATGGGTGGCCTCACAATTCCTAGCACAGAAGATGTTTTTTGCATGTTACTTCTAGCTTCAATGGACTATAAACCTGCAGCTGCGAGAGACTTACATTGA
Protein sequence	>MS.gene047517.t1 MGFRIPAAITRATFSSSQTSSKVANVPKGYLAVYVGEEMKRFVIPTSYLNQTSFQYLLSRSEEEFRYDHPMGGLTIPSTEDVFCMLLLASMDYKPAAARDLH