AlfalfaGEDB | MS.gene003596

M. sativa cultivar XinJiangDaYe / MS.gene003596

KEGG: K14488
GO: GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
PFAM: PF02519.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene003596.t1	RHN61427.1	88.4	86	10	0	1	86	42	127	2.20E-37	164.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene003596.t1	P33081	73.8	84	20	1	1	84	1	82	1.9e-29	129.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene003596.t1	G7JL56	88.4	86	10	0	1	86	1	86	1.6e-37	164.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene003596	MS.gene003630	PPI
MS.gene003580	MS.gene003596	PPI
MS.gene003582	MS.gene003596	PPI
MS.gene003584	MS.gene003596	PPI
MS.gene057800	MS.gene003596	PPI
MS.gene003596	MS.gene062965	PPI
MS.gene003596	MS.gene50771	PPI
MS.gene003596	MS.gene057802	PPI
MS.gene50772	MS.gene003596	PPI
MS.gene062966	MS.gene003596	PPI
MS.gene003596	MS.gene062986	PPI
MS.gene057784	MS.gene003596	PPI
MS.gene057783	MS.gene003596	PPI
MS.gene062978	MS.gene003596	PPI
MS.gene50688	MS.gene003596	PPI
MS.gene003596	MS.gene50658	PPI
MS.gene003596	MS.gene50655	PPI
MS.gene003596	MS.gene28863	PPI
MS.gene50662	MS.gene003596	PPI
MS.gene50683	MS.gene003596	PPI
MS.gene003596	MS.gene057804	PPI
MS.gene003628	MS.gene003596	PPI
MS.gene003599	MS.gene003596	PPI
MS.gene057807	MS.gene003596	PPI
MS.gene003607	MS.gene003596	PPI
MS.gene003585	MS.gene003596	PPI
MS.gene23368	MS.gene003596	PPI
MS.gene003581	MS.gene003596	PPI
MS.gene003596	MS.gene50666	PPI
MS.gene003596	MS.gene062985	PPI
MS.gene057796	MS.gene003596	PPI
MS.gene50653	MS.gene003596	PPI
MS.gene057816	MS.gene003596	PPI
MS.gene003603	MS.gene003596	PPI
MS.gene003596	MS.gene062953	PPI
MS.gene062946	MS.gene003596	PPI
MS.gene062944	MS.gene003596	PPI
MS.gene057799	MS.gene003596	PPI
MS.gene003596	MS.gene057817	PPI
MS.gene057780	MS.gene003596	PPI
MS.gene057788	MS.gene003596	PPI
MS.gene50773	MS.gene003596	PPI
MS.gene057811	MS.gene003596	PPI
MS.gene003596	MS.gene70684	PPI
MS.gene003596	MS.gene062964	PPI
MS.gene062954	MS.gene003596	PPI
MS.gene50657	MS.gene003596	PPI
MS.gene003596	MS.gene062958	PPI
MS.gene003596	MS.gene003612	PPI
MS.gene003596	MS.gene062983	PPI
MS.gene50651	MS.gene003596	PPI
MS.gene003596	MS.gene057777	PPI
MS.gene50685	MS.gene003596	PPI
MS.gene062962	MS.gene003596	PPI
MS.gene003596	MS.gene062945	PPI
MS.gene77525	MS.gene003596	PPI
MS.gene003576	MS.gene003596	PPI
MS.gene003596	MS.gene003595	PPI
MS.gene062969	MS.gene003596	PPI
MS.gene003596	MS.gene50656	PPI
MS.gene057782	MS.gene003596	PPI
MS.gene003596	MS.gene50686	PPI
MS.gene23369	MS.gene003596	PPI
MS.gene003596	MS.gene057785	PPI
MS.gene003596	MS.gene70683	PPI
MS.gene003596	MS.gene50670	PPI
MS.gene003626	MS.gene003596	PPI
MS.gene003598	MS.gene003596	PPI
MS.gene057791	MS.gene003596	PPI
MS.gene003596	MS.gene70685	PPI
MS.gene062967	MS.gene003596	PPI
MS.gene003596	MS.gene003622	PPI
MS.gene057805	MS.gene003596	PPI
MS.gene062942	MS.gene003596	PPI
MS.gene003605	MS.gene003596	PPI
MS.gene003596	MS.gene062941	PPI
MS.gene057798	MS.gene003596	PPI
MS.gene003596	MS.gene50660	PPI
MS.gene062971	MS.gene003596	PPI
MS.gene23373	MS.gene003596	PPI
MS.gene003596	MS.gene003623	PPI
MS.gene003596	MS.gene50668	PPI
MS.gene062989	MS.gene003596	PPI
MS.gene062987	MS.gene003596	PPI
MS.gene062980	MS.gene003596	PPI
MS.gene003596	MS.gene057794	PPI
MS.gene062975	MS.gene003596	PPI
MS.gene50689	MS.gene003596	PPI
MS.gene003596	MS.gene23367	PPI
MS.gene50770	MS.gene003596	PPI
MS.gene50674	MS.gene003596	PPI
MS.gene003596	MS.gene062959	PPI
MS.gene003606	MS.gene003596	PPI
MS.gene003596	MS.gene50664	PPI
MS.gene062940	MS.gene003596	PPI
MS.gene057806	MS.gene003596	PPI
MS.gene003596	MS.gene50682	PPI
MS.gene057809	MS.gene003596	PPI
MS.gene50673	MS.gene003596	PPI
MS.gene062970	MS.gene003596	PPI
MS.gene003596	MS.gene50690	PPI
MS.gene057779	MS.gene003596	PPI
MS.gene50669	MS.gene003596	PPI
MS.gene003629	MS.gene003596	PPI
MS.gene003597	MS.gene003596	PPI
MS.gene022358	MS.gene003596	PPI
MS.gene003596	MS.gene003613	PPI
MS.gene003593	MS.gene003596	PPI
MS.gene003579	MS.gene003596	PPI
MS.gene062972	MS.gene003596	PPI
MS.gene003610	MS.gene003596	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene003596.t1	MTR_4g072610	88.372	86	10	0	1	86	1	86	4.86e-54	162
MS.gene003596.t1	MTR_4g072650	86.047	86	12	0	1	86	1	86	6.58e-52	157
MS.gene003596.t1	MTR_4g072580	79.310	87	17	1	1	86	1	87	1.71e-47	146
MS.gene003596.t1	MTR_3g084180	71.111	90	20	1	1	84	1	90	4.03e-44	137
MS.gene003596.t1	MTR_4g072740	76.471	85	19	1	1	84	1	85	7.70e-44	137
MS.gene003596.t1	MTR_3g084150	70.000	90	21	1	1	84	1	90	6.98e-43	134
MS.gene003596.t1	MTR_4g072490	75.294	85	20	1	1	84	1	85	1.59e-42	133
MS.gene003596.t1	MTR_4g072370	72.222	90	21	2	1	86	1	90	1.85e-42	133
MS.gene003596.t1	MTR_4g072600	73.563	87	22	1	1	86	1	87	9.15e-42	131
MS.gene003596.t1	MTR_4g072470	74.118	85	21	1	1	84	1	85	1.31e-41	131
MS.gene003596.t1	MTR_4g072820	73.810	84	21	1	1	83	1	84	2.49e-41	130
MS.gene003596.t1	MTR_4g072630	73.256	86	21	1	1	84	1	86	2.52e-41	130
MS.gene003596.t1	MTR_8g461410	67.778	90	23	1	1	84	1	90	2.76e-41	130
MS.gene003596.t1	MTR_4g072830	74.157	89	19	2	1	85	1	89	2.96e-41	130
MS.gene003596.t1	MTR_3g110038	69.231	91	22	2	1	85	1	91	5.21e-40	127
MS.gene003596.t1	MTR_4g072465	69.767	86	24	1	1	84	1	86	7.40e-40	127
MS.gene003596.t1	MTR_3g084170	68.889	90	22	2	1	84	1	90	6.36e-39	124
MS.gene003596.t1	MTR_3g110032	68.132	91	22	2	1	84	1	91	4.37e-38	122
MS.gene003596.t1	MTR_4g072310	65.934	91	24	1	1	84	1	91	6.70e-38	122
MS.gene003596.t1	MTR_4g072330	65.556	90	27	2	1	86	1	90	7.07e-38	122
MS.gene003596.t1	MTR_3g084240	67.778	90	23	2	1	84	1	90	7.10e-38	122
MS.gene003596.t1	MTR_4g072880	67.033	91	23	2	1	84	1	91	1.41e-37	121
MS.gene003596.t1	MTR_4g072670	70.930	86	23	1	1	84	1	86	1.95e-37	120
MS.gene003596.t1	MTR_3g084260	67.033	91	23	2	1	84	1	91	2.52e-37	120
MS.gene003596.t1	MTR_3g084220	66.667	90	24	2	1	84	1	90	3.16e-37	120
MS.gene003596.t1	MTR_4g072510	72.093	86	22	1	1	84	1	86	3.34e-37	120
MS.gene003596.t1	MTR_3g110042	66.292	89	24	1	1	83	1	89	5.40e-37	120
MS.gene003596.t1	MTR_4g072540	69.767	86	24	1	1	84	1	86	1.15e-36	119
MS.gene003596.t1	MTR_3g117630	65.934	91	25	1	1	85	1	91	1.19e-36	119
MS.gene003596.t1	MTR_4g072870	62.637	91	27	2	1	84	1	91	1.96e-36	118
MS.gene003596.t1	MTR_4g072720	75.714	70	17	0	15	84	17	86	2.40e-36	118
MS.gene003596.t1	MTR_4g072220	60.870	92	29	1	1	85	1	92	2.73e-36	118
MS.gene003596.t1	MTR_3g084250	63.333	90	27	1	1	84	1	90	2.92e-36	118
MS.gene003596.t1	MTR_4g072260	61.538	91	28	1	1	84	1	91	7.64e-36	117
MS.gene003596.t1	MTR_4g072850	64.516	93	25	1	1	85	1	93	9.08e-36	116
MS.gene003596.t1	MTR_4g072860	68.132	91	22	2	1	84	1	91	1.20e-35	116
MS.gene003596.t1	MTR_4g072240	60.440	91	29	1	1	84	1	91	1.25e-35	116
MS.gene003596.t1	MTR_4g072250	67.033	91	22	2	1	84	1	90	1.50e-35	116
MS.gene003596.t1	MTR_4g072890	60.440	91	29	1	1	84	1	91	1.77e-35	115
MS.gene003596.t1	MTR_4g050700	63.736	91	26	2	1	84	1	91	3.67e-35	115
MS.gene003596.t1	MTR_2g012410	63.736	91	26	2	1	84	1	91	3.67e-35	115
MS.gene003596.t1	MTR_4g072590	70.588	85	24	1	1	84	1	85	6.20e-35	114
MS.gene003596.t1	MTR_4g072270	64.773	88	24	1	1	81	1	88	6.85e-35	114
MS.gene003596.t1	MTR_4g072300	59.341	91	30	1	1	84	1	91	9.70e-35	114
MS.gene003596.t1	MTR_3g084190	63.736	91	27	1	1	85	1	91	1.01e-34	114
MS.gene003596.t1	MTR_4g072480	62.222	90	28	1	1	84	1	90	1.43e-34	113
MS.gene003596.t1	MTR_8g461420	62.637	91	28	1	1	85	1	91	1.80e-34	113
MS.gene003596.t1	MTR_3g110045	64.045	89	26	1	3	85	62	150	2.57e-34	115
MS.gene003596.t1	MTR_4g072500	62.222	90	28	1	1	84	1	90	3.48e-34	112
MS.gene003596.t1	MTR_4g072380	68.675	83	24	2	1	81	1	83	5.51e-34	112
MS.gene003596.t1	MTR_4g072460	61.111	90	29	1	1	84	1	90	5.83e-34	112
MS.gene003596.t1	MTR_3g084230	61.538	91	29	1	1	85	1	91	8.28e-34	111
MS.gene003596.t1	MTR_4g072570	62.222	90	28	1	1	84	1	90	1.10e-33	111
MS.gene003596.t1	MTR_4g072230	66.292	89	21	3	1	82	1	87	1.28e-33	111
MS.gene003596.t1	MTR_4g072620	60.000	90	30	1	1	84	1	90	1.93e-33	110
MS.gene003596.t1	MTR_4g072640	61.538	91	29	1	1	85	1	91	2.65e-33	110
MS.gene003596.t1	MTR_4g072680	61.111	90	29	1	1	84	1	90	3.23e-33	110
MS.gene003596.t1	MTR_5g008350	68.421	76	23	1	1	75	1	76	5.23e-33	109
MS.gene003596.t1	MTR_4g072530	60.000	90	30	1	1	84	1	90	1.12e-32	108
MS.gene003596.t1	MTR_3g084160	61.538	91	29	1	1	85	1	91	1.69e-32	108
MS.gene003596.t1	MTR_3g084210	58.889	90	31	1	1	84	1	90	2.22e-32	108
MS.gene003596.t1	MTR_4g072730	58.889	90	31	1	1	84	1	90	2.29e-32	108
MS.gene003596.t1	MTR_4g072660	63.415	82	29	1	1	81	1	82	2.34e-32	108
MS.gene003596.t1	MTR_5g008360	65.333	75	26	0	10	84	7	81	6.06e-32	106
MS.gene003596.t1	MTR_4g072690	64.706	85	29	1	1	84	1	85	4.05e-31	104
MS.gene003596.t1	MTR_4g072940	61.538	91	27	1	3	85	33	123	5.86e-31	105
MS.gene003596.t1	MTR_4g072450	64.706	85	29	1	1	84	1	85	2.76e-30	102
MS.gene003596.t1	MTR_5g008370	74.627	67	17	0	18	84	24	90	4.63e-30	103
MS.gene003596.t1	MTR_4g072770	60.241	83	29	1	1	83	1	79	2.11e-29	100
MS.gene003596.t1	MTR_4g072980	52.174	92	35	2	1	83	1	92	2.59e-28	98.2
MS.gene003596.t1	MTR_3g084200	66.667	72	18	2	1	66	1	72	4.03e-28	97.8
MS.gene003596.t1	MTR_4g072550	55.952	84	37	0	1	84	18	101	6.41e-28	97.4
MS.gene003596.t1	MTR_3g117640	58.571	70	28	1	18	86	33	102	1.04e-26	94.4
MS.gene003596.t1	MTR_4g072560	60.294	68	26	1	18	84	31	98	1.12e-26	94.0
MS.gene003596.t1	MTR_4g072910	60.000	75	29	1	10	84	27	100	3.04e-26	92.8
MS.gene003596.t1	MTR_4g072750	58.108	74	23	1	1	66	1	74	2.04e-22	82.8
MS.gene003596.t1	MTR_4g072290	51.765	85	32	2	1	84	1	77	7.59e-22	81.3
MS.gene003596.t1	MTR_4g072930	47.500	80	38	1	9	84	31	110	5.77e-21	80.1
MS.gene003596.t1	MTR_3g113310	49.254	67	33	1	17	83	40	105	4.82e-20	77.4
MS.gene003596.t1	MTR_3g110035	54.688	64	29	0	21	84	73	136	7.01e-20	77.8
MS.gene003596.t1	MTR_6g077850	47.059	68	36	0	18	85	33	100	1.84e-19	75.9
MS.gene003596.t1	MTR_3g117610	50.725	69	33	1	17	85	43	110	2.55e-19	75.9
MS.gene003596.t1	MTR_4g072280	49.333	75	31	1	1	68	1	75	8.21e-19	73.6
MS.gene003596.t1	MTR_4g072190	44.872	78	38	2	8	81	21	97	2.31e-18	73.2
MS.gene003596.t1	MTR_5g021820	45.570	79	40	2	7	83	26	103	1.09e-17	71.2
MS.gene003596.t1	MTR_4g072840	46.988	83	20	1	1	83	1	59	3.90e-17	68.9
MS.gene003596.t1	MTR_3g105900	82.927	41	7	0	18	58	1171	1211	1.80e-16	72.8
MS.gene003596.t1	MTR_8g461400	45.556	90	17	3	1	84	1	64	2.47e-15	64.3
MS.gene003596.t1	MTR_8g076040	48.438	64	32	1	18	81	40	102	2.64e-15	65.5
MS.gene003596.t1	MTR_2g043960	50.847	59	28	1	18	76	75	132	5.83e-15	65.9
MS.gene003596.t1	MTR_4g124750	52.542	59	27	1	18	76	68	125	8.07e-15	65.5
MS.gene003596.t1	MTR_1g061570	49.315	73	34	2	9	79	7	78	8.64e-15	64.7
MS.gene003596.t1	MTR_1g009150	46.377	69	36	1	11	79	63	130	6.58e-14	63.5
MS.gene003596.t1	MTR_4g005310	43.548	62	34	1	19	79	62	123	1.01e-13	62.4
MS.gene003596.t1	MTR_4g005320	45.902	61	32	1	19	79	70	129	8.15e-13	60.5
MS.gene003596.t1	MTR_1g094750	46.774	62	32	1	18	79	49	109	1.96e-12	58.2
MS.gene003596.t1	MTR_8g461210	44.118	68	37	1	18	85	34	100	3.32e-12	57.8
MS.gene003596.t1	MTR_4g025830	41.667	72	38	2	16	84	76	146	3.97e-12	58.5
MS.gene003596.t1	MTR_1g024085	44.118	68	37	1	18	85	36	102	6.16e-12	57.8
MS.gene003596.t1	MTR_4g072160	40.260	77	45	1	9	85	27	102	8.83e-12	57.0
MS.gene003596.t1	MTR_7g101740	45.312	64	34	1	18	81	39	101	5.15e-11	54.7
MS.gene003596.t1	MTR_0388s0020	36.471	85	50	2	3	84	59	142	9.32e-11	54.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene003596.t1	AT4G38840	66.176	68	22	1	18	84	32	99	1.59e-30	103
MS.gene003596.t1	AT5G18020	57.333	75	31	1	12	85	17	91	3.23e-28	97.8
MS.gene003596.t1	AT5G18080	60.274	73	28	1	12	83	17	89	4.76e-28	97.4
MS.gene003596.t1	AT5G18010	58.904	73	29	1	12	83	17	89	1.01e-27	96.3
MS.gene003596.t1	AT5G18060	64.615	65	22	1	20	83	25	89	1.59e-27	95.9
MS.gene003596.t1	AT5G18050	63.636	66	23	1	19	83	24	89	2.87e-27	95.1
MS.gene003596.t1	AT5G18030	58.904	73	29	1	12	83	15	87	4.30e-27	94.7
MS.gene003596.t1	AT2G21200	56.579	76	32	1	9	83	11	86	8.19e-27	94.0
MS.gene003596.t1	AT2G21210	53.947	76	33	1	11	84	20	95	1.17e-26	94.0
MS.gene003596.t1	AT4G38850	58.228	79	26	2	8	83	14	88	2.16e-26	93.2
MS.gene003596.t1	AT4G38825	59.155	71	28	1	14	83	18	88	2.78e-26	92.8
MS.gene003596.t1	AT2G21210	53.947	76	33	1	11	84	87	162	7.90e-26	94.0
MS.gene003596.t1	AT3G03840	51.765	85	34	2	1	83	14	93	2.60e-25	90.5
MS.gene003596.t1	AT3G03850	62.121	66	23	1	20	83	26	91	4.23e-24	87.4
MS.gene003596.t1	AT4G34810	57.143	70	25	2	19	83	48	117	6.80e-24	87.4
MS.gene003596.t1	AT4G13790	47.436	78	39	1	8	83	14	91	7.04e-24	86.7
MS.gene003596.t1	AT4G34800	54.795	73	30	1	10	79	12	84	1.39e-23	86.3
MS.gene003596.t1	AT3G03820	52.632	76	33	1	11	83	19	94	2.08e-23	85.9
MS.gene003596.t1	AT4G34770	59.420	69	26	1	18	84	34	102	3.92e-23	85.1
MS.gene003596.t1	AT3G03830	50.000	84	39	1	3	83	8	91	1.57e-22	83.2
MS.gene003596.t1	AT4G34780	52.778	72	31	1	17	85	26	97	8.80e-21	79.3
MS.gene003596.t1	AT4G38860	52.308	65	31	0	17	81	38	102	2.44e-20	78.2
MS.gene003596.t1	AT4G34790	52.308	65	29	1	19	81	39	103	2.01e-19	75.9
MS.gene003596.t1	AT2G21220	49.231	65	32	1	17	81	38	101	1.07e-17	71.6
MS.gene003596.t1	AT1G75580	50.746	67	32	1	17	83	42	107	1.39e-17	71.2
MS.gene003596.t1	AT4G34760	49.231	65	32	1	17	81	41	104	1.99e-17	70.9
MS.gene003596.t1	AT2G16580	49.231	65	32	1	17	81	42	105	4.92e-17	69.7
MS.gene003596.t1	AT4G36110	48.438	64	32	1	18	81	39	101	3.72e-16	67.4
MS.gene003596.t1	AT3G20220	45.679	81	40	3	7	86	37	114	4.60e-16	67.8
MS.gene003596.t1	AT1G19830	51.471	68	31	2	17	83	46	112	7.95e-15	64.3
MS.gene003596.t1	AT3G43120	49.153	59	29	1	18	76	78	135	8.29e-15	65.5
MS.gene003596.t1	AT2G18010	45.312	64	34	1	18	81	47	109	9.79e-15	64.3
MS.gene003596.t1	AT4G34750	42.029	69	39	1	18	86	36	103	3.95e-14	63.5
MS.gene003596.t1	AT4G34750	42.029	69	39	1	18	86	36	103	3.95e-14	63.5
MS.gene003596.t1	AT5G20810	49.153	59	29	1	18	76	78	135	9.89e-14	62.8
MS.gene003596.t1	AT5G20810	49.153	59	29	1	18	76	78	135	2.02e-13	62.4
MS.gene003596.t1	AT2G37030	47.619	63	32	1	18	80	48	109	3.47e-12	57.8
MS.gene003596.t1	AT5G66260	43.939	66	36	1	19	84	34	98	9.20e-12	56.2
MS.gene003596.t1	AT3G53250	39.189	74	41	1	7	80	40	109	3.71e-11	55.1
MS.gene003596.t1	AT1G75590	44.068	59	32	1	18	76	41	98	3.72e-11	55.8
MS.gene003596.t1	AT5G53590	39.726	73	38	2	18	85	45	116	4.92e-11	55.5
MS.gene003596.t1	AT3G53250	39.189	74	41	1	7	80	71	140	5.79e-11	55.5
MS.gene003596.t1	AT3G61900	36.905	84	48	3	6	85	16	98	9.02e-11	54.7

Find 26 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAGCCAGGTGTGGAAGAATT+TGG	0.271966	4.1:+30861429	MS.gene003596:CDS
AGCCAGGTGTGGAAGAATTT+GGG	0.408494	4.1:+30861430	MS.gene003596:CDS
GGCGTCATCTTCAAAAGGCT+TGG	0.459638	4.1:+30861306	MS.gene003596:CDS
ATTCCAAGACTTGCTGAGCC+AGG	0.465187	4.1:+30861414	MS.gene003596:CDS
ACACCTGGCTCAGCAAGTCT+TGG	0.469392	4.1:-30861417	None:intergenic
AGATGACGCCCTTCTGATAC+CGG	0.480930	4.1:-30861293	None:intergenic
TTCATTTACCCGGTATCAGA+AGG	0.487678	4.1:+30861284	MS.gene003596:CDS
GTTTGATTCAAATATGACAT+TGG	0.515716	4.1:-30861388	None:intergenic
TACACTGCAAGGTATCCCTT+TGG	0.521748	4.1:-30861334	None:intergenic
AAAAGGCTTGGATGTGCCAA+AGG	0.530838	4.1:+30861318	MS.gene003596:CDS
AGAAGGGCGTCATCTTCAAA+AGG	0.535977	4.1:+30861301	MS.gene003596:CDS
GGATACCTTGCAGTGTATGT+AGG	0.553208	4.1:+30861340	MS.gene003596:CDS
TTGGGATATGAACATCCAAT+GGG	0.558278	4.1:+30861448	MS.gene003596:CDS
TTTGGGATATGAACATCCAA+TGG	0.564158	4.1:+30861447	MS.gene003596:CDS
ATCCCAAATTCTTCCACACC+TGG	0.565399	4.1:-30861432	None:intergenic
GGTGGTCTTACTATTCCTTG+TGG	0.578653	4.1:+30861469	MS.gene003596:CDS
AAAGGCTTGGATGTGCCAAA+GGG	0.593194	4.1:+30861319	MS.gene003596:CDS
ATATCACTTCTCGCTTGAGT+AGG	0.598317	4.1:+30861509	MS.gene003596:CDS
GATGACGCCCTTCTGATACC+GGG	0.608185	4.1:-30861292	None:intergenic
TCATTTACCCGGTATCAGAA+GGG	0.616131	4.1:+30861285	MS.gene003596:CDS
GGAATAGTAAGACCACCCAT+TGG	0.616831	4.1:-30861463	None:intergenic
AAGACTTGCTGAGCCAGGTG+TGG	0.621679	4.1:+30861419	MS.gene003596:CDS
TCTCTCCTACATACACTGCA+AGG	0.634902	4.1:-30861345	None:intergenic
ACGAAAAGATCTTCTCCACA+AGG	0.667214	4.1:-30861484	None:intergenic
GGATATGAACATCCAATGGG+TGG	0.669309	4.1:+30861451	MS.gene003596:CDS
ATGTAGGAGAGAAAATGAAG+CGG	0.712638	4.1:+30861356	MS.gene003596:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	GTTTGATTCAAATATGACAT+TGG	-	chr4.1:30861391-30861410	None:intergenic	25.0%
	ATGTAGGAGAGAAAATGAAG+CGG	+	chr4.1:30861356-30861375	MS.gene003596:CDS	35.0%
	TTGGGATATGAACATCCAAT+GGG	+	chr4.1:30861448-30861467	MS.gene003596:CDS	35.0%
!	TTTGGGATATGAACATCCAA+TGG	+	chr4.1:30861447-30861466	MS.gene003596:CDS	35.0%
	ACGAAAAGATCTTCTCCACA+AGG	-	chr4.1:30861487-30861506	None:intergenic	40.0%
	ATATCACTTCTCGCTTGAGT+AGG	+	chr4.1:30861509-30861528	MS.gene003596:CDS	40.0%
	TCATTTACCCGGTATCAGAA+GGG	+	chr4.1:30861285-30861304	MS.gene003596:CDS	40.0%
	TTCATTTACCCGGTATCAGA+AGG	+	chr4.1:30861284-30861303	MS.gene003596:CDS	40.0%
	AAAAGGCTTGGATGTGCCAA+AGG	+	chr4.1:30861318-30861337	MS.gene003596:CDS	45.0%
	AAAGGCTTGGATGTGCCAAA+GGG	+	chr4.1:30861319-30861338	MS.gene003596:CDS	45.0%
	AGCCAGGTGTGGAAGAATTT+GGG	+	chr4.1:30861430-30861449	MS.gene003596:CDS	45.0%
	ATCCCAAATTCTTCCACACC+TGG	-	chr4.1:30861435-30861454	None:intergenic	45.0%
	GGAATAGTAAGACCACCCAT+TGG	-	chr4.1:30861466-30861485	None:intergenic	45.0%
	GGATACCTTGCAGTGTATGT+AGG	+	chr4.1:30861340-30861359	MS.gene003596:CDS	45.0%
	GGATATGAACATCCAATGGG+TGG	+	chr4.1:30861451-30861470	MS.gene003596:CDS	45.0%
	TCTCTCCTACATACACTGCA+AGG	-	chr4.1:30861348-30861367	None:intergenic	45.0%
!	GGTGGTCTTACTATTCCTTG+TGG	+	chr4.1:30861469-30861488	MS.gene003596:CDS	45.0%
!	TACACTGCAAGGTATCCCTT+TGG	-	chr4.1:30861337-30861356	None:intergenic	45.0%
!!	AGAAGGGCGTCATCTTCAAA+AGG	+	chr4.1:30861301-30861320	MS.gene003596:CDS	45.0%
	AGATGACGCCCTTCTGATAC+CGG	-	chr4.1:30861296-30861315	None:intergenic	50.0%
	GAGCCAGGTGTGGAAGAATT+TGG	+	chr4.1:30861429-30861448	MS.gene003596:CDS	50.0%
	GGCGTCATCTTCAAAAGGCT+TGG	+	chr4.1:30861306-30861325	MS.gene003596:CDS	50.0%
!!	ATTCCAAGACTTGCTGAGCC+AGG	+	chr4.1:30861414-30861433	MS.gene003596:CDS	50.0%
	ACACCTGGCTCAGCAAGTCT+TGG	-	chr4.1:30861420-30861439	None:intergenic	55.0%
	GATGACGCCCTTCTGATACC+GGG	-	chr4.1:30861295-30861314	None:intergenic	55.0%
!!	AAGACTTGCTGAGCCAGGTG+TGG	+	chr4.1:30861419-30861438	MS.gene003596:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	30861277	30861537	30861277	ID=MS.gene003596
chr4.1	mRNA	30861277	30861537	30861277	ID=MS.gene003596.t1;Parent=MS.gene003596
chr4.1	exon	30861277	30861537	30861277	ID=MS.gene003596.t1.exon1;Parent=MS.gene003596.t1
chr4.1	CDS	30861277	30861537	30861277	ID=cds.MS.gene003596.t1;Parent=MS.gene003596.t1

Gene Sequence	>MS.gene003596 ATGGGTTTTCATTTACCCGGTATCAGAAGGGCGTCATCTTCAAAAGGCTTGGATGTGCCAAAGGGATACCTTGCAGTGTATGTAGGAGAGAAAATGAAGCGGTTTGTGATTCCAATGTCATATTTGAATCAAACTTCATTCCAAGACTTGCTGAGCCAGGTGTGGAAGAATTTGGGATATGAACATCCAATGGGTGGTCTTACTATTCCTTGTGGAGAAGATCTTTTCGTAGATATCACTTCTCGCTTGAGTAGGTGCTAA
Protein sequence	>MS.gene003596.t1 MGFHLPGIRRASSSKGLDVPKGYLAVYVGEKMKRFVIPMSYLNQTSFQDLLSQVWKNLGYEHPMGGLTIPCGEDLFVDITSRLSRC