AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene031199

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031199.t1 MTR_3g110038 96.739 92 3 0 1 92 1 92 2.01e-63 186
MS.gene031199.t1 MTR_3g084170 93.478 92 6 0 1 92 1 92 2.34e-61 181
MS.gene031199.t1 MTR_3g084240 92.391 92 7 0 1 92 1 92 2.01e-60 179
MS.gene031199.t1 MTR_3g084220 91.304 92 8 0 1 92 1 92 1.40e-59 177
MS.gene031199.t1 MTR_3g084150 84.946 93 12 2 1 92 1 92 1.35e-54 164
MS.gene031199.t1 MTR_4g050700 83.871 93 14 1 1 92 1 93 2.46e-54 164
MS.gene031199.t1 MTR_2g012410 83.871 93 14 1 1 92 1 93 2.46e-54 164
MS.gene031199.t1 MTR_8g461410 83.871 93 13 2 1 92 1 92 2.91e-54 163
MS.gene031199.t1 MTR_3g084180 83.871 93 13 2 1 92 1 92 1.23e-53 162
MS.gene031199.t1 MTR_3g110032 81.720 93 16 1 1 92 1 93 6.79e-52 157
MS.gene031199.t1 MTR_3g084260 82.418 91 15 1 1 90 1 91 5.18e-51 155
MS.gene031199.t1 MTR_3g110042 82.955 88 13 2 1 87 1 87 4.58e-49 150
MS.gene031199.t1 MTR_3g117630 78.889 90 18 1 4 92 3 92 1.30e-48 149
MS.gene031199.t1 MTR_4g072250 74.194 93 22 2 1 92 1 92 4.90e-47 145
MS.gene031199.t1 MTR_4g072310 72.043 93 25 1 1 92 1 93 5.28e-47 145
MS.gene031199.t1 MTR_4g072880 70.968 93 26 1 1 92 1 93 1.34e-46 144
MS.gene031199.t1 MTR_4g072270 72.043 93 25 1 1 92 1 93 2.59e-46 143
MS.gene031199.t1 MTR_4g072870 71.429 91 25 1 1 90 1 91 1.61e-45 141
MS.gene031199.t1 MTR_4g072370 74.444 90 21 1 1 90 1 88 1.62e-45 141
MS.gene031199.t1 MTR_4g072330 73.333 90 22 1 1 90 1 88 3.19e-45 140
MS.gene031199.t1 MTR_3g084200 90.278 72 7 0 1 72 1 72 1.14e-44 139
MS.gene031199.t1 MTR_4g072240 71.429 91 25 1 1 90 1 91 1.86e-44 139
MS.gene031199.t1 MTR_4g072860 72.043 93 25 1 1 92 1 93 1.98e-44 139
MS.gene031199.t1 MTR_4g072220 70.968 93 26 1 1 92 1 93 2.43e-44 138
MS.gene031199.t1 MTR_4g072260 69.892 93 27 1 1 92 1 93 3.06e-44 138
MS.gene031199.t1 MTR_4g072740 72.222 90 20 1 1 90 1 85 3.91e-44 137
MS.gene031199.t1 MTR_4g072300 71.429 91 25 1 1 90 1 91 6.08e-44 137
MS.gene031199.t1 MTR_4g072230 72.043 93 23 2 1 92 1 91 6.36e-44 137
MS.gene031199.t1 MTR_4g072470 72.222 90 20 1 1 90 1 85 8.26e-44 137
MS.gene031199.t1 MTR_4g072830 75.281 89 20 1 1 89 1 87 1.22e-43 136
MS.gene031199.t1 MTR_4g072890 71.429 91 25 1 1 90 1 91 1.33e-43 136
MS.gene031199.t1 MTR_4g072490 70.000 90 22 1 1 90 1 85 1.54e-43 136
MS.gene031199.t1 MTR_4g072580 71.111 90 21 1 1 90 1 85 2.08e-43 136
MS.gene031199.t1 MTR_4g072640 72.826 92 23 2 1 91 1 91 6.35e-43 135
MS.gene031199.t1 MTR_4g072500 70.968 93 25 2 1 92 1 92 7.90e-43 134
MS.gene031199.t1 MTR_4g072850 71.277 94 25 1 1 92 1 94 1.40e-42 134
MS.gene031199.t1 MTR_4g072630 70.000 90 23 1 1 90 1 86 3.32e-42 133
MS.gene031199.t1 MTR_4g072530 70.330 91 25 2 1 90 1 90 1.04e-41 132
MS.gene031199.t1 MTR_4g072730 66.667 90 30 0 1 90 1 90 1.06e-41 132
MS.gene031199.t1 MTR_4g072610 71.111 90 20 2 1 90 1 84 1.32e-41 131
MS.gene031199.t1 MTR_4g072480 65.217 92 32 0 1 92 1 92 1.69e-41 131
MS.gene031199.t1 MTR_4g072620 64.130 92 33 0 1 92 1 92 5.22e-41 130
MS.gene031199.t1 MTR_3g084250 67.742 93 28 2 1 92 1 92 5.95e-41 130
MS.gene031199.t1 MTR_4g072680 65.217 92 32 0 1 92 1 92 1.04e-40 129
MS.gene031199.t1 MTR_4g072465 66.667 90 26 1 1 90 1 86 1.09e-40 129
MS.gene031199.t1 MTR_4g072650 71.111 90 20 2 1 90 1 84 1.20e-40 129
MS.gene031199.t1 MTR_4g072570 65.217 92 32 0 1 92 1 92 2.17e-40 128
MS.gene031199.t1 MTR_4g072670 68.889 90 24 1 1 90 1 86 1.27e-39 126
MS.gene031199.t1 MTR_4g072540 71.111 90 22 1 1 90 1 86 1.30e-39 126
MS.gene031199.t1 MTR_4g072660 65.217 92 27 1 1 92 1 87 3.87e-39 125
MS.gene031199.t1 MTR_4g072460 63.043 92 34 0 1 92 1 92 3.93e-39 125
MS.gene031199.t1 MTR_4g072600 68.966 87 22 1 1 87 1 82 4.93e-39 125
MS.gene031199.t1 MTR_4g072820 67.778 90 24 2 1 90 1 85 1.05e-38 124
MS.gene031199.t1 MTR_4g072510 68.889 90 24 1 1 90 1 86 1.13e-38 124
MS.gene031199.t1 MTR_4g072940 69.565 92 26 1 3 92 33 124 8.15e-38 123
MS.gene031199.t1 MTR_3g084210 63.043 92 34 0 1 92 1 92 9.77e-38 122
MS.gene031199.t1 MTR_4g072380 67.778 90 25 1 1 90 1 86 1.31e-37 121
MS.gene031199.t1 MTR_3g084190 63.043 92 34 0 1 92 1 92 1.42e-37 121
MS.gene031199.t1 MTR_4g072720 66.667 90 26 1 1 90 1 86 2.93e-37 120
MS.gene031199.t1 MTR_4g072590 67.778 90 24 1 1 90 1 85 4.27e-37 120
MS.gene031199.t1 MTR_8g461420 60.870 92 36 0 1 92 1 92 8.38e-37 119
MS.gene031199.t1 MTR_3g084230 60.870 92 36 0 1 92 1 92 2.48e-36 118
MS.gene031199.t1 MTR_3g084160 61.957 92 35 0 1 92 1 92 4.44e-36 117
MS.gene031199.t1 MTR_5g008350 69.620 79 19 1 1 79 1 74 5.75e-36 117
MS.gene031199.t1 MTR_4g072690 67.778 90 24 1 1 90 1 85 6.84e-36 117
MS.gene031199.t1 MTR_3g110045 63.333 90 33 0 3 92 62 151 1.39e-35 118
MS.gene031199.t1 MTR_5g008360 71.622 74 21 0 19 92 10 83 1.42e-35 116
MS.gene031199.t1 MTR_8g461400 66.304 92 5 1 1 92 1 66 1.55e-35 115
MS.gene031199.t1 MTR_5g008370 63.333 90 33 0 1 90 1 90 4.89e-35 116
MS.gene031199.t1 MTR_4g072450 65.556 90 26 1 1 90 1 85 4.09e-34 112
MS.gene031199.t1 MTR_4g072980 58.696 92 35 2 1 89 1 92 4.43e-34 112
MS.gene031199.t1 MTR_4g072550 60.870 92 33 1 4 92 12 103 6.80e-34 112
MS.gene031199.t1 MTR_4g072560 52.041 98 39 3 1 90 1 98 5.95e-31 105
MS.gene031199.t1 MTR_4g072770 69.697 66 20 0 25 90 15 80 2.68e-30 102
MS.gene031199.t1 MTR_4g072910 63.380 71 26 0 20 90 30 100 5.50e-30 102
MS.gene031199.t1 MTR_3g117640 50.000 100 40 2 1 90 1 100 1.66e-29 101
MS.gene031199.t1 MTR_4g072290 55.435 92 28 2 1 92 1 79 1.70e-28 98.2
MS.gene031199.t1 MTR_4g072280 64.000 75 26 1 1 74 1 75 5.90e-27 94.4
MS.gene031199.t1 MTR_3g110035 63.636 66 24 0 27 92 73 138 8.80e-26 93.2
MS.gene031199.t1 MTR_4g072750 62.667 75 24 2 1 72 1 74 5.71e-25 89.7
MS.gene031199.t1 MTR_3g113310 56.923 65 27 1 23 87 40 103 8.66e-24 87.0
MS.gene031199.t1 MTR_4g072930 47.059 85 40 2 11 90 26 110 3.69e-23 85.9
MS.gene031199.t1 MTR_3g117610 58.462 65 26 1 23 87 43 106 6.21e-23 85.1
MS.gene031199.t1 MTR_6g077850 52.239 67 32 0 24 90 33 99 2.22e-22 83.6
MS.gene031199.t1 MTR_4g072190 48.193 83 41 2 5 87 17 97 4.23e-22 82.8
MS.gene031199.t1 MTR_4g072840 51.724 87 12 4 1 87 1 57 2.36e-19 74.7
MS.gene031199.t1 MTR_5g021820 51.562 64 30 1 24 87 39 101 1.11e-18 74.3
MS.gene031199.t1 MTR_8g076040 51.562 64 30 1 24 87 40 102 1.40e-18 73.9
MS.gene031199.t1 MTR_2g043960 53.226 62 28 1 24 85 75 135 3.34e-18 74.3
MS.gene031199.t1 MTR_4g005310 46.053 76 40 1 11 85 48 123 4.25e-18 73.9
MS.gene031199.t1 MTR_1g094750 48.235 85 39 2 5 85 26 109 6.01e-18 72.8
MS.gene031199.t1 MTR_4g124750 51.613 62 29 1 24 85 68 128 2.63e-17 72.0
MS.gene031199.t1 MTR_1g009150 54.839 62 27 1 24 85 70 130 1.58e-16 70.5
MS.gene031199.t1 MTR_4g005320 52.459 61 28 1 25 85 70 129 7.49e-16 68.6
MS.gene031199.t1 MTR_0388s0020 44.286 70 35 2 11 77 61 129 1.11e-14 65.1
MS.gene031199.t1 MTR_3g105900 80.556 36 7 0 24 59 1171 1206 2.33e-14 67.0
MS.gene031199.t1 MTR_8g461210 43.023 86 41 3 4 82 7 91 3.53e-14 63.2
MS.gene031199.t1 MTR_3g109160 54.098 61 27 1 25 85 69 128 4.75e-14 63.9
MS.gene031199.t1 MTR_1g024085 47.761 67 34 1 16 82 28 93 1.04e-13 62.4
MS.gene031199.t1 MTR_7g118010 42.466 73 39 2 17 87 15 86 1.05e-13 61.6
MS.gene031199.t1 MTR_4g072160 47.059 68 32 2 24 88 36 102 2.70e-13 61.2
MS.gene031199.t1 MTR_3g097120 40.244 82 40 2 18 91 2 82 3.49e-13 60.1
MS.gene031199.t1 MTR_3g092220 43.478 69 36 2 16 82 24 91 7.28e-13 59.7
MS.gene031199.t1 MTR_3g098970 32.500 80 53 1 13 92 41 119 8.82e-13 60.1
MS.gene031199.t1 MTR_1g061570 48.387 62 31 1 24 85 18 78 1.16e-12 59.3
MS.gene031199.t1 MTR_1g063950 38.750 80 46 2 8 85 29 107 2.04e-12 58.5
MS.gene031199.t1 MTR_7g101740 45.312 64 34 1 24 87 39 101 2.72e-12 58.2
MS.gene031199.t1 MTR_8g026730 43.077 65 34 2 25 87 27 90 3.36e-12 57.8
MS.gene031199.t1 MTR_7g405740 45.902 61 32 1 25 85 67 126 5.58e-12 58.5
MS.gene031199.t1 MTR_8g101520 37.079 89 50 2 8 91 28 115 6.62e-12 58.2
MS.gene031199.t1 MTR_4g021810 43.038 79 41 3 5 82 8 83 9.05e-12 56.6
MS.gene031199.t1 MTR_8g096470 43.333 60 33 1 26 85 47 105 4.74e-11 54.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031199.t1 AT4G38840 72.059 68 18 1 24 90 32 99 1.15e-34 114
MS.gene031199.t1 AT5G18020 63.158 76 27 1 16 90 15 90 1.59e-32 108
MS.gene031199.t1 AT5G18050 61.842 76 28 1 16 90 15 90 9.01e-32 107
MS.gene031199.t1 AT5G18060 61.842 76 28 1 16 90 15 90 2.24e-31 105
MS.gene031199.t1 AT5G18080 67.164 67 21 1 25 90 24 90 7.07e-31 104
MS.gene031199.t1 AT5G18030 65.672 67 22 1 25 90 22 88 1.44e-30 103
MS.gene031199.t1 AT2G21200 62.500 72 26 1 19 89 15 86 5.97e-30 102
MS.gene031199.t1 AT2G21210 56.410 78 32 1 15 90 18 95 1.20e-29 101
MS.gene031199.t1 AT4G38825 61.111 72 27 1 20 90 18 89 1.82e-29 101
MS.gene031199.t1 AT5G18010 59.211 76 30 1 16 90 15 90 1.87e-29 101
MS.gene031199.t1 AT4G34770 72.727 66 16 1 19 82 29 94 2.47e-29 101
MS.gene031199.t1 AT2G21210 56.410 78 32 1 15 90 85 162 9.06e-29 101
MS.gene031199.t1 AT3G03840 59.722 72 27 1 21 90 23 94 2.75e-27 95.9
MS.gene031199.t1 AT4G34800 53.488 86 33 2 1 83 1 82 4.43e-27 95.1
MS.gene031199.t1 AT4G34810 61.765 68 22 1 24 87 47 114 5.53e-27 95.9
MS.gene031199.t1 AT4G38850 55.422 83 25 2 9 88 14 87 7.67e-27 94.4
MS.gene031199.t1 AT3G03820 52.874 87 38 1 7 90 9 95 8.27e-27 94.7
MS.gene031199.t1 AT3G03850 62.687 67 23 1 26 90 26 92 1.85e-26 93.6
MS.gene031199.t1 AT3G03830 49.451 91 38 2 8 90 2 92 7.21e-26 92.0
MS.gene031199.t1 AT4G13790 50.000 82 34 2 9 88 14 90 1.05e-24 89.0
MS.gene031199.t1 AT4G34780 54.545 77 32 1 17 90 20 96 2.45e-24 88.6
MS.gene031199.t1 AT4G34790 63.492 63 21 1 25 85 39 101 7.96e-24 87.4
MS.gene031199.t1 AT4G38860 56.923 65 28 0 23 87 38 102 5.59e-23 85.1
MS.gene031199.t1 AT1G75580 52.703 74 34 1 14 87 33 105 4.23e-22 83.2
MS.gene031199.t1 AT4G34760 55.385 65 28 1 23 87 41 104 4.95e-21 80.1
MS.gene031199.t1 AT2G21220 52.308 65 30 1 23 87 38 101 1.83e-20 78.6
MS.gene031199.t1 AT4G36110 51.515 66 31 1 24 89 39 103 1.83e-19 76.3
MS.gene031199.t1 AT3G20220 54.545 66 28 2 25 89 47 111 2.59e-19 76.3
MS.gene031199.t1 AT2G16580 52.308 65 30 1 23 87 42 105 4.30e-19 75.5
MS.gene031199.t1 AT2G18010 50.000 66 32 1 24 89 47 111 1.21e-18 74.3
MS.gene031199.t1 AT3G43120 51.613 62 29 1 24 85 78 138 1.72e-18 75.1
MS.gene031199.t1 AT1G19830 58.333 60 24 1 23 82 46 104 2.48e-18 73.6
MS.gene031199.t1 AT5G20810 53.226 62 28 1 24 85 78 138 1.30e-17 73.2
MS.gene031199.t1 AT5G20810 53.226 62 28 1 24 85 78 138 3.11e-17 72.8
MS.gene031199.t1 AT5G66260 42.391 92 49 2 2 90 8 98 1.56e-15 66.2
MS.gene031199.t1 AT5G10990 47.222 72 37 1 11 82 27 97 2.59e-15 66.6
MS.gene031199.t1 AT1G75590 47.619 84 38 3 4 82 16 98 3.41e-15 66.6
MS.gene031199.t1 AT4G34750 41.026 78 45 1 10 87 22 98 3.65e-15 66.2
MS.gene031199.t1 AT4G34750 41.026 78 45 1 10 87 22 98 3.65e-15 66.2
MS.gene031199.t1 AT1G19840 45.783 83 40 2 4 82 16 97 2.12e-14 64.7
MS.gene031199.t1 AT3G51200 50.820 61 28 2 24 83 38 97 4.32e-14 62.4
MS.gene031199.t1 AT2G46690 41.892 74 39 2 17 87 16 88 5.32e-14 62.8
MS.gene031199.t1 AT2G24400 50.000 66 32 1 20 85 59 123 8.69e-14 63.5
MS.gene031199.t1 AT4G31320 50.820 61 29 1 25 85 80 139 9.87e-14 63.5
MS.gene031199.t1 AT2G37030 39.744 78 44 2 11 86 33 109 1.75e-13 61.6
MS.gene031199.t1 AT3G61900 38.095 84 48 2 10 90 15 97 3.59e-13 60.8
MS.gene031199.t1 AT3G12830 40.000 75 44 1 11 85 36 109 1.23e-12 59.3
MS.gene031199.t1 AT5G53590 39.726 73 38 2 24 91 45 116 5.60e-12 58.2
MS.gene031199.t1 AT3G53250 42.857 63 32 1 24 86 51 109 7.92e-12 57.4
MS.gene031199.t1 AT4G00880 46.970 66 32 2 24 87 27 91 1.32e-11 56.6
MS.gene031199.t1 AT3G53250 42.857 63 32 1 24 86 82 140 1.56e-11 57.0
MS.gene031199.t1 AT1G16510 42.029 69 39 1 14 82 43 110 2.95e-11 56.2

Find 0 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATTCAATACTTTTGATGTT+TGG - 21207:20262-20281 None:intergenic 25.0%
!! AAAAGTATTGAATGTGCCAA+AGG + 21207:20268-20287 MS.gene031199:CDS 30.0%
!! AAAGTATTGAATGTGCCAAA+GGG + 21207:20269-20288 MS.gene031199:CDS 30.0%
!! TCAAAACTTGTTGAATCAAG+TGG + 21207:20367-20386 MS.gene031199:CDS 30.0%
!!! ACAAGTTTTGAAATGAAGCT+TGG - 21207:20358-20377 None:intergenic 30.0%
AAGAAAACATCTTCTGTGCA+AGG - 21207:20437-20456 None:intergenic 35.0%
AATCAAGTGGAGGAAGAATT+CGG + 21207:20380-20399 MS.gene031199:CDS 35.0%
ATTCGGATATGATCATCCAA+TGG + 21207:20397-20416 MS.gene031199:CDS 35.0%
TTCGGATATGATCATCCAAT+GGG + 21207:20398-20417 MS.gene031199:CDS 35.0%
!! AAACTTGTTGAATCAAGTGG+AGG + 21207:20370-20389 MS.gene031199:CDS 35.0%
AGCTTGGTTCAAGTATGACA+TGG - 21207:20342-20361 None:intergenic 40.0%
CTTGGTTCAAGTATGACATG+GGG - 21207:20340-20359 None:intergenic 40.0%
GCTTGGTTCAAGTATGACAT+GGG - 21207:20341-20360 None:intergenic 40.0%
GTCTAATAGCTGCAGGTAAA+CGG - 21207:20220-20239 None:intergenic 40.0%
TACACTGCAAGATAACCCTT+TGG - 21207:20287-20306 None:intergenic 40.0%
ACGTTGGAGAGCAAATGAAG+CGG + 21207:20306-20325 MS.gene031199:CDS 45.0%
CTTCTGTGCAAGGAATTGTG+AGG - 21207:20427-20446 None:intergenic 45.0%
GGATATGATCATCCAATGGG+TGG + 21207:20401-20420 MS.gene031199:CDS 45.0%
GGTTATCTTGCAGTGTACGT+TGG + 21207:20290-20309 MS.gene031199:CDS 45.0%
GAAGCTCGTCTAATAGCTGC+AGG - 21207:20227-20246 None:intergenic 50.0%
GGAATTGTGAGGCCACCCAT+TGG - 21207:20416-20435 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
21207 gene 20209 20487 20209 ID=MS.gene031199
21207 mRNA 20209 20487 20209 ID=MS.gene031199.t1;Parent=MS.gene031199
21207 exon 20209 20487 20209 ID=MS.gene031199.t1.exon1;Parent=MS.gene031199.t1
21207 CDS 20209 20487 20209 ID=cds.MS.gene031199.t1;Parent=MS.gene031199.t1
Gene Sequence

>MS.gene031199

ATGGGTTTCCGTTTACCTGCAGCTATTAGACGAGCTTCATTTAGCAGTAGCCAAACATCAAAAGTATTGAATGTGCCAAAGGGTTATCTTGCAGTGTACGTTGGAGAGCAAATGAAGCGGTTTGTTATCCCCATGTCATACTTGAACCAAGCTTCATTTCAAAACTTGTTGAATCAAGTGGAGGAAGAATTCGGATATGATCATCCAATGGGTGGCCTCACAATTCCTTGCACAGAAGATGTTTTCTTGCATATTACTGCTCGCTTCAATGAGCTATAA
Protein sequence

>MS.gene031199.t1

MGFRLPAAIRRASFSSSQTSKVLNVPKGYLAVYVGEQMKRFVIPMSYLNQASFQNLLNQVEEEFGYDHPMGGLTIPCTEDVFLHITARFNEL