AlfalfaGEDB | MS.gene062963

M. sativa cultivar XinJiangDaYe / MS.gene062963

KEGG: K14488
GO: GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
PFAM: PF02519.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062963.t1	AES89319.1	90.6	85	8	0	40	124	1	85	5.10E-35	157.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062963.t1	P33079	69.2	91	22	1	40	124	1	91	2.3e-28	126.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062963.t1	G7JL44	90.6	85	8	0	40	124	1	85	3.7e-35	157.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene057808	MS.gene062963	0.971753	1.16E-133	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene062963	MS.gene50660	PPI
MS.gene062963	MS.gene057799	PPI
MS.gene062963	MS.gene50688	PPI
MS.gene062963	MS.gene003630	PPI
MS.gene003603	MS.gene062963	PPI
MS.gene062946	MS.gene062963	PPI
MS.gene057805	MS.gene062963	PPI
MS.gene062963	MS.gene062980	PPI
MS.gene062963	MS.gene003610	PPI
MS.gene062963	MS.gene50771	PPI
MS.gene062971	MS.gene062963	PPI
MS.gene23373	MS.gene062963	PPI
MS.gene062963	MS.gene022358	PPI
MS.gene062963	MS.gene057802	PPI
MS.gene062954	MS.gene062963	PPI
MS.gene50657	MS.gene062963	PPI
MS.gene062963	MS.gene057796	PPI
MS.gene062963	MS.gene50682	PPI
MS.gene062975	MS.gene062963	PPI
MS.gene50689	MS.gene062963	PPI
MS.gene003576	MS.gene062963	PPI
MS.gene062963	MS.gene057780	PPI
MS.gene062963	MS.gene057788	PPI
MS.gene062969	MS.gene062963	PPI
MS.gene062963	MS.gene062944	PPI
MS.gene057782	MS.gene062963	PPI
MS.gene003606	MS.gene062963	PPI
MS.gene062963	MS.gene50655	PPI
MS.gene062963	MS.gene28863	PPI
MS.gene062963	MS.gene50685	PPI
MS.gene062963	MS.gene003613	PPI
MS.gene062963	MS.gene003584	PPI
MS.gene062963	MS.gene057791	PPI
MS.gene003626	MS.gene062963	PPI
MS.gene50673	MS.gene062963	PPI
MS.gene062970	MS.gene062963	PPI
MS.gene062942	MS.gene062963	PPI
MS.gene062963	MS.gene057779	PPI
MS.gene003605	MS.gene062963	PPI
MS.gene062963	MS.gene062965	PPI
MS.gene062963	MS.gene50666	PPI
MS.gene003597	MS.gene062963	PPI
MS.gene062963	MS.gene062953	PPI
MS.gene062972	MS.gene062963	PPI
MS.gene062963	MS.gene23369	PPI
MS.gene062963	MS.gene062986	PPI
MS.gene062963	MS.gene50770	PPI
MS.gene062963	MS.gene062987	PPI
MS.gene003582	MS.gene062963	PPI
MS.gene062963	MS.gene057811	PPI
MS.gene062963	MS.gene057817	PPI
MS.gene057800	MS.gene062963	PPI
MS.gene062963	MS.gene70684	PPI
MS.gene50674	MS.gene062963	PPI
MS.gene062963	MS.gene062964	PPI
MS.gene062963	MS.gene057777	PPI
MS.gene062963	MS.gene062945	PPI
MS.gene057806	MS.gene062963	PPI
MS.gene057809	MS.gene062963	PPI
MS.gene062963	MS.gene057804	PPI
MS.gene062966	MS.gene062963	PPI
MS.gene062963	MS.gene003579	PPI
MS.gene062963	MS.gene062989	PPI
MS.gene062963	MS.gene50651	PPI
MS.gene062978	MS.gene062963	PPI
MS.gene062963	MS.gene003607	PPI
MS.gene062963	MS.gene50656	PPI
MS.gene062963	MS.gene50686	PPI
MS.gene062963	MS.gene70683	PPI
MS.gene062963	MS.gene50658	PPI
MS.gene062963	MS.gene062985	PPI
MS.gene062963	MS.gene057784	PPI
MS.gene003593	MS.gene062963	PPI
MS.gene062963	MS.gene50669	PPI
MS.gene062963	MS.gene003622	PPI
MS.gene003628	MS.gene062963	PPI
MS.gene003580	MS.gene062963	PPI
MS.gene062963	MS.gene003599	PPI
MS.gene062963	MS.gene23368	PPI
MS.gene003585	MS.gene062963	PPI
MS.gene062963	MS.gene003623	PPI
MS.gene062963	MS.gene50668	PPI
MS.gene062963	MS.gene003581	PPI
MS.gene062963	MS.gene062958	PPI
MS.gene062963	MS.gene057798	PPI
MS.gene062963	MS.gene003612	PPI
MS.gene062963	MS.gene057794	PPI
MS.gene062963	MS.gene003629	PPI
MS.gene062963	MS.gene062983	PPI
MS.gene057816	MS.gene062963	PPI
MS.gene50772	MS.gene062963	PPI
MS.gene062963	MS.gene062967	PPI
MS.gene062963	MS.gene23367	PPI
MS.gene062963	MS.gene062940	PPI
MS.gene062963	MS.gene003595	PPI
MS.gene057783	MS.gene062963	PPI
MS.gene062963	MS.gene062959	PPI
MS.gene062963	MS.gene50664	PPI
MS.gene062963	MS.gene057785	PPI
MS.gene062963	MS.gene50773	PPI
MS.gene062963	MS.gene50670	PPI
MS.gene062962	MS.gene062963	PPI
MS.gene062963	MS.gene50653	PPI
MS.gene062963	MS.gene003598	PPI
MS.gene50662	MS.gene062963	PPI
MS.gene062963	MS.gene70685	PPI
MS.gene50683	MS.gene062963	PPI
MS.gene062963	MS.gene50690	PPI
MS.gene77525	MS.gene062963	PPI
MS.gene057807	MS.gene062963	PPI
MS.gene062963	MS.gene062941	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062963.t1	MTR_4g072490	90.588	85	8	0	40	124	1	85	1.57e-52	160
MS.gene062963.t1	MTR_4g072740	88.235	85	10	0	40	124	1	85	3.21e-51	157
MS.gene062963.t1	MTR_4g072630	86.047	86	11	1	40	124	1	86	2.02e-48	150
MS.gene062963.t1	MTR_4g072465	84.884	86	12	1	40	124	1	86	4.54e-48	149
MS.gene062963.t1	MTR_4g072580	83.529	85	14	0	40	124	1	85	6.02e-48	149
MS.gene062963.t1	MTR_4g072470	82.353	85	15	0	40	124	1	85	8.70e-48	148
MS.gene062963.t1	MTR_4g072830	81.609	87	13	1	40	123	1	87	4.48e-46	144
MS.gene062963.t1	MTR_4g072370	79.545	88	15	1	40	124	1	88	7.03e-46	144
MS.gene062963.t1	MTR_4g072670	86.047	86	11	1	40	124	1	86	4.34e-45	141
MS.gene062963.t1	MTR_4g072600	78.824	85	18	0	40	124	1	85	1.24e-43	138
MS.gene062963.t1	MTR_4g072330	72.727	88	21	1	40	124	1	88	3.29e-43	137
MS.gene062963.t1	MTR_4g072720	84.884	86	12	1	40	124	1	86	5.89e-43	136
MS.gene062963.t1	MTR_3g084150	74.725	91	16	2	40	124	1	90	9.77e-43	135
MS.gene062963.t1	MTR_4g072540	83.721	86	13	1	40	124	1	86	1.17e-42	135
MS.gene062963.t1	MTR_4g072610	77.647	85	18	1	40	124	1	84	2.10e-42	135
MS.gene062963.t1	MTR_4g072510	83.721	86	13	1	40	124	1	86	2.24e-42	134
MS.gene062963.t1	MTR_3g084170	72.222	90	20	1	40	124	1	90	2.45e-42	135
MS.gene062963.t1	MTR_8g461410	73.626	91	17	2	40	124	1	90	2.71e-42	134
MS.gene062963.t1	MTR_3g084180	73.626	91	17	2	40	124	1	90	6.87e-42	134
MS.gene062963.t1	MTR_4g072650	77.647	85	18	1	40	124	1	84	1.04e-41	133
MS.gene062963.t1	MTR_3g084240	71.111	90	21	1	40	124	1	90	2.58e-41	132
MS.gene062963.t1	MTR_3g110032	70.330	91	21	1	40	124	1	91	8.23e-41	131
MS.gene062963.t1	MTR_4g072690	81.176	85	16	0	40	124	1	85	9.33e-41	130
MS.gene062963.t1	MTR_3g110038	70.000	90	22	1	40	124	1	90	9.83e-41	130
MS.gene062963.t1	MTR_4g072590	81.176	85	16	0	40	124	1	85	1.08e-40	130
MS.gene062963.t1	MTR_3g084220	70.000	90	22	1	40	124	1	90	1.09e-40	130
MS.gene062963.t1	MTR_5g008350	82.432	74	13	0	40	113	1	74	1.43e-40	130
MS.gene062963.t1	MTR_4g072450	81.176	85	16	0	40	124	1	85	2.88e-40	129
MS.gene062963.t1	MTR_4g072880	71.429	91	20	1	40	124	1	91	3.61e-40	129
MS.gene062963.t1	MTR_4g050700	69.231	91	22	1	40	124	1	91	5.47e-40	129
MS.gene062963.t1	MTR_2g012410	69.231	91	22	1	40	124	1	91	5.47e-40	129
MS.gene062963.t1	MTR_4g072380	74.419	86	21	1	40	124	1	86	3.22e-39	127
MS.gene062963.t1	MTR_3g110042	75.000	88	15	2	40	121	1	87	3.42e-39	127
MS.gene062963.t1	MTR_4g072310	68.132	91	23	1	40	124	1	91	3.93e-39	127
MS.gene062963.t1	MTR_4g072250	71.429	91	19	2	40	124	1	90	1.09e-38	125
MS.gene062963.t1	MTR_4g072870	67.033	91	24	1	40	124	1	91	1.29e-38	125
MS.gene062963.t1	MTR_4g072820	74.419	86	20	2	40	124	1	85	1.91e-38	125
MS.gene062963.t1	MTR_3g084260	68.132	91	23	1	40	124	1	91	3.83e-38	124
MS.gene062963.t1	MTR_4g072850	73.913	92	17	1	40	124	1	92	6.11e-38	124
MS.gene062963.t1	MTR_4g072240	65.934	91	25	1	40	124	1	91	8.48e-38	123
MS.gene062963.t1	MTR_4g072480	66.667	90	25	1	40	124	1	90	7.30e-37	121
MS.gene062963.t1	MTR_4g072860	68.132	91	23	1	40	124	1	91	7.85e-37	121
MS.gene062963.t1	MTR_3g117630	69.318	88	21	1	43	124	3	90	1.83e-36	120
MS.gene062963.t1	MTR_4g072220	64.835	91	26	1	40	124	1	91	2.27e-36	120
MS.gene062963.t1	MTR_4g072890	64.835	91	26	1	40	124	1	91	2.31e-36	119
MS.gene062963.t1	MTR_4g072570	66.667	90	25	1	40	124	1	90	3.03e-36	119
MS.gene062963.t1	MTR_4g072270	65.934	91	25	1	40	124	1	91	3.12e-36	119
MS.gene062963.t1	MTR_3g084250	66.667	90	25	1	40	124	1	90	3.53e-36	119
MS.gene062963.t1	MTR_4g072300	63.736	91	27	1	40	124	1	91	5.37e-36	119
MS.gene062963.t1	MTR_4g072620	64.444	90	27	1	40	124	1	90	5.41e-36	119
MS.gene062963.t1	MTR_4g072260	63.736	91	27	1	40	124	1	91	1.44e-35	117
MS.gene062963.t1	MTR_4g072230	67.033	91	22	2	40	124	1	89	1.79e-35	117
MS.gene062963.t1	MTR_4g072680	64.444	90	27	1	40	124	1	90	2.40e-35	117
MS.gene062963.t1	MTR_4g072660	63.529	85	31	0	40	124	1	85	6.54e-35	115
MS.gene062963.t1	MTR_4g072500	63.333	90	28	1	40	124	1	90	7.64e-35	115
MS.gene062963.t1	MTR_4g072730	62.222	90	29	1	40	124	1	90	2.14e-34	115
MS.gene062963.t1	MTR_4g072530	62.222	90	29	1	40	124	1	90	3.12e-34	114
MS.gene062963.t1	MTR_4g072640	63.333	90	28	1	40	124	1	90	4.40e-34	114
MS.gene062963.t1	MTR_4g072460	62.222	90	29	1	40	124	1	90	6.82e-34	113
MS.gene062963.t1	MTR_3g084210	63.333	90	28	1	40	124	1	90	9.26e-34	113
MS.gene062963.t1	MTR_3g084190	65.556	90	26	1	40	124	1	90	1.29e-33	112
MS.gene062963.t1	MTR_3g084160	64.444	90	27	1	40	124	1	90	2.01e-33	112
MS.gene062963.t1	MTR_8g461420	64.444	90	27	1	40	124	1	90	2.79e-33	112
MS.gene062963.t1	MTR_5g008360	65.385	78	27	0	47	124	4	81	5.84e-33	110
MS.gene062963.t1	MTR_3g084230	63.333	90	28	1	40	124	1	90	7.99e-33	110
MS.gene062963.t1	MTR_4g072940	70.000	90	20	1	42	124	33	122	1.62e-32	111
MS.gene062963.t1	MTR_3g110045	61.957	92	30	1	38	124	58	149	1.91e-31	109
MS.gene062963.t1	MTR_5g008370	76.471	68	16	0	57	124	23	90	2.31e-30	106
MS.gene062963.t1	MTR_3g084200	68.056	72	18	1	40	106	1	72	7.28e-30	103
MS.gene062963.t1	MTR_4g072560	50.515	97	35	2	40	123	1	97	8.39e-29	100
MS.gene062963.t1	MTR_4g072980	52.174	92	36	2	40	123	1	92	2.18e-28	99.8
MS.gene062963.t1	MTR_4g072550	64.789	71	25	0	54	124	31	101	3.60e-27	97.1
MS.gene062963.t1	MTR_4g072770	66.667	66	22	0	59	124	15	80	1.04e-26	95.1
MS.gene062963.t1	MTR_4g072910	59.722	72	29	0	53	124	29	100	2.42e-26	94.7
MS.gene062963.t1	MTR_3g117640	46.000	100	39	2	40	124	1	100	2.69e-26	94.7
MS.gene062963.t1	MTR_4g072290	55.294	85	30	1	40	124	1	77	2.15e-24	89.4
MS.gene062963.t1	MTR_3g110035	62.500	64	24	0	61	124	73	136	9.64e-23	86.7
MS.gene062963.t1	MTR_4g072750	60.274	73	22	1	40	105	1	73	4.17e-22	83.6
MS.gene062963.t1	MTR_4g072280	62.687	67	19	1	40	100	1	67	4.94e-22	83.2
MS.gene062963.t1	MTR_4g072930	47.500	80	37	2	50	124	31	110	2.47e-19	77.4
MS.gene062963.t1	MTR_3g117610	53.846	65	29	1	57	121	43	106	2.59e-19	77.0
MS.gene062963.t1	MTR_6g077850	49.254	67	34	0	58	124	33	99	5.82e-19	75.9
MS.gene062963.t1	MTR_4g072190	48.101	79	36	2	47	121	20	97	6.85e-19	75.9
MS.gene062963.t1	MTR_3g113310	49.254	67	33	1	57	123	40	105	1.15e-18	75.5
MS.gene062963.t1	MTR_8g461400	46.667	90	17	2	40	124	1	64	3.55e-18	73.2
MS.gene062963.t1	MTR_4g072840	49.412	85	18	2	40	124	1	60	8.91e-18	72.0
MS.gene062963.t1	MTR_2g043960	55.932	59	25	1	58	116	75	132	6.01e-16	70.1
MS.gene062963.t1	MTR_4g124750	54.237	59	26	1	58	116	68	125	8.23e-16	69.3
MS.gene062963.t1	MTR_5g021820	48.485	66	33	1	58	123	39	103	1.51e-15	67.4
MS.gene062963.t1	MTR_8g076040	50.000	64	31	1	58	121	40	102	6.28e-15	65.9
MS.gene062963.t1	MTR_1g009150	50.725	69	33	1	51	119	63	130	1.57e-14	66.6
MS.gene062963.t1	MTR_1g094750	51.613	62	29	1	58	119	49	109	7.76e-14	63.2
MS.gene062963.t1	MTR_7g101740	46.875	64	33	1	58	121	39	101	2.87e-13	61.6
MS.gene062963.t1	MTR_3g105900	63.636	44	16	0	56	99	1169	1212	3.16e-13	65.1
MS.gene062963.t1	MTR_1g061570	51.613	62	29	1	58	119	18	78	5.77e-13	61.2
MS.gene062963.t1	MTR_1g063950	43.548	62	34	1	58	119	47	107	1.25e-12	60.5
MS.gene062963.t1	MTR_4g005320	47.541	61	31	1	59	119	70	129	2.11e-12	60.8
MS.gene062963.t1	MTR_3g092220	35.556	90	55	2	37	124	11	99	2.40e-12	59.3
MS.gene062963.t1	MTR_7g118010	46.970	66	32	2	58	121	22	86	3.35e-12	58.9
MS.gene062963.t1	MTR_1g024085	50.847	59	28	1	58	116	36	93	3.53e-12	59.7
MS.gene062963.t1	MTR_5g008380	84.375	32	5	0	93	124	246	277	8.22e-12	60.5
MS.gene062963.t1	MTR_4g005310	40.580	69	40	1	52	119	55	123	8.45e-12	58.9
MS.gene062963.t1	MTR_4g072160	43.662	71	39	1	49	119	27	96	2.86e-11	57.4
MS.gene062963.t1	MTR_8g026730	41.176	68	37	2	59	124	27	93	4.50e-11	55.8
MS.gene062963.t1	MTR_3g109160	49.180	61	30	1	59	119	69	128	6.04e-11	57.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062963.t1	AT4G38840	73.529	68	17	1	58	124	32	99	1.85e-31	107
MS.gene062963.t1	AT2G21210	59.211	76	29	1	51	124	20	95	1.59e-27	97.8
MS.gene062963.t1	AT4G38850	59.259	81	26	2	47	124	13	89	1.75e-27	97.4
MS.gene062963.t1	AT5G18080	57.500	80	31	2	46	124	13	90	2.95e-27	96.7
MS.gene062963.t1	AT5G18050	56.410	78	33	1	48	124	13	90	2.98e-27	96.7
MS.gene062963.t1	AT5G18060	57.692	78	32	1	48	124	13	90	4.51e-27	96.3
MS.gene062963.t1	AT2G21210	49.485	97	39	2	38	124	66	162	5.13e-27	98.6
MS.gene062963.t1	AT4G34810	63.291	79	24	2	50	123	39	117	5.37e-27	97.1
MS.gene062963.t1	AT5G18020	55.000	80	33	2	46	124	13	90	1.44e-26	95.1
MS.gene062963.t1	AT2G21200	55.696	79	34	1	46	123	8	86	2.45e-26	94.4
MS.gene062963.t1	AT5G18030	56.250	80	30	2	46	124	13	88	3.15e-26	94.4
MS.gene062963.t1	AT4G34800	55.952	84	33	2	40	119	1	84	6.42e-26	93.6
MS.gene062963.t1	AT4G38825	58.333	72	29	1	54	124	18	89	9.85e-26	92.8
MS.gene062963.t1	AT5G18010	53.750	80	34	2	46	124	13	90	5.43e-25	91.3
MS.gene062963.t1	AT3G03840	46.000	100	46	2	27	124	1	94	1.63e-24	90.1
MS.gene062963.t1	AT4G13790	49.367	79	38	1	47	123	13	91	9.89e-24	87.8
MS.gene062963.t1	AT3G03820	53.247	77	33	1	51	124	19	95	6.88e-23	85.9
MS.gene062963.t1	AT4G34770	56.000	75	31	1	52	124	28	102	1.01e-22	85.9
MS.gene062963.t1	AT3G03830	55.263	76	31	1	52	124	17	92	1.57e-22	84.7
MS.gene062963.t1	AT3G03850	61.194	67	24	1	60	124	26	92	2.76e-22	84.3
MS.gene062963.t1	AT4G34790	62.295	61	21	1	59	117	39	99	1.01e-20	80.9
MS.gene062963.t1	AT4G34780	52.778	72	31	1	56	124	25	96	9.17e-20	78.2
MS.gene062963.t1	AT4G38860	44.000	100	43	2	35	124	9	105	9.23e-20	78.2
MS.gene062963.t1	AT2G21220	43.000	100	43	3	35	124	9	104	1.96e-18	74.7
MS.gene062963.t1	AT1G75580	41.860	86	49	1	38	123	23	107	1.38e-17	72.8
MS.gene062963.t1	AT4G34760	50.769	65	31	1	57	121	41	104	4.49e-17	71.2
MS.gene062963.t1	AT2G16580	50.769	65	31	1	57	121	42	105	1.74e-16	70.1
MS.gene062963.t1	AT4G36110	48.438	64	32	1	58	121	39	101	1.34e-15	67.4
MS.gene062963.t1	AT5G20810	55.932	59	25	1	58	116	78	135	2.03e-15	68.6
MS.gene062963.t1	AT5G20810	55.932	59	25	1	58	116	78	135	3.46e-15	68.6
MS.gene062963.t1	AT3G43120	52.542	59	27	1	58	116	78	135	5.44e-15	67.4
MS.gene062963.t1	AT2G18010	48.438	64	32	1	58	121	47	109	5.64e-15	66.2
MS.gene062963.t1	AT1G19830	52.941	68	30	2	57	123	46	112	5.71e-15	66.2
MS.gene062963.t1	AT5G66260	46.269	67	35	1	58	124	33	98	6.26e-13	60.5
MS.gene062963.t1	AT2G46690	44.444	72	36	2	48	116	13	83	7.96e-13	60.8
MS.gene062963.t1	AT2G37030	47.619	63	32	1	58	120	48	109	1.88e-12	60.1
MS.gene062963.t1	AT3G61900	38.554	83	47	2	45	124	16	97	1.89e-12	60.1
MS.gene062963.t1	AT4G34750	39.189	74	44	1	48	121	26	98	2.35e-12	60.5
MS.gene062963.t1	AT4G34750	39.189	74	44	1	48	121	26	98	2.35e-12	60.5
MS.gene062963.t1	AT1G75590	47.458	59	30	1	58	116	41	98	6.36e-12	59.3
MS.gene062963.t1	AT1G19840	45.714	70	35	2	49	116	29	97	1.72e-11	58.2
MS.gene062963.t1	AT3G51200	41.772	79	40	3	46	119	22	99	2.00e-11	57.0
MS.gene062963.t1	AT5G10990	43.478	69	35	2	48	116	33	97	2.14e-11	57.8
MS.gene062963.t1	AT4G31320	42.466	73	40	2	48	119	68	139	6.27e-11	57.4
MS.gene062963.t1	AT2G24400	45.205	73	38	2	48	119	52	123	7.11e-11	57.0

Find 23 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAGCTTGGCTCAACAATTCT+TGG	0.248657	4.2:+35491396	None:intergenic
ACAATTCTTGGAATGAGGTT+TGG	0.392315	4.2:+35491408	None:intergenic
GATGTCCTTCTGATGATAAT+AGG	0.401454	4.2:+35491524	None:intergenic
TTCTGATGATAATAGGTAAA+CGG	0.431960	4.2:+35491531	None:intergenic
GTTTGGTTCAAATATGATAC+AGG	0.443189	4.2:+35491425	None:intergenic
GCTCAACAATTCTTGGAATG+AGG	0.458184	4.2:+35491403	None:intergenic
ATTCAAATTGTTCCTCAGCT+TGG	0.481982	4.2:+35491381	None:intergenic
TACACTGCTAGATATCCCTT+TGG	0.484780	4.2:+35491479	None:intergenic
GACATCATCTTCAAAAGTTG+TGG	0.499899	4.2:-35491507	MS.gene062963:CDS
TTTGAATACGACCCTCCAAT+GGG	0.519750	4.2:-35491365	MS.gene062963:CDS
AAGAAAATTTCTTCTCTGCA+AGG	0.519764	4.2:+35491329	None:intergenic
AAAAGTTGTGGATGTTCCAA+AGG	0.527225	4.2:-35491495	MS.gene062963:CDS
AAAGTTGTGGATGTTCCAAA+GGG	0.543795	4.2:-35491494	MS.gene062963:CDS
ATTTGAATACGACCCTCCAA+TGG	0.545953	4.2:-35491366	MS.gene062963:CDS
GTTTACCTATTATCATCAGA+AGG	0.577855	4.2:-35491529	MS.gene062963:CDS
TGATAATGCTGATGTCCAGC+TGG	0.597209	4.2:-35491954	MS.gene062963:intron
TGATAATGCTGATGTCCAGC+TGG	0.597209	4.2:-35492105	MS.gene062963:intron
GGAATAGTGAGACCACCCAT+TGG	0.603922	4.2:+35491350	None:intergenic
ATAGTGAGACCACCCATTGG+AGG	0.607611	4.2:+35491353	None:intergenic
TAGTGAGACCACCCATTGGA+GGG	0.635609	4.2:+35491354	None:intergenic
AGAATTGTTGAGCCAAGCTG+AGG	0.641874	4.2:-35491393	MS.gene062963:CDS
GAATACGACCCTCCAATGGG+TGG	0.665793	4.2:-35491362	MS.gene062963:CDS
ATGTAAGTGAGAAAATGAAG+CGG	0.680120	4.2:-35491457	MS.gene062963:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTCATCTATATATATTTA+TGG	-	chr4.2:35491745-35491764	MS.gene062963:intron	10.0%
!!!	TTTTTAACACATACAACAAT+GGG	-	chr4.2:35491878-35491897	MS.gene062963:intron	20.0%
!	AAAGATATGTAACAAACAGT+CGG	+	chr4.2:35491728-35491747	None:intergenic	25.0%
!	ATGTGTTAAAAAGAATGGTT+TGG	+	chr4.2:35491870-35491889	None:intergenic	25.0%
!	GTTGTATGTGTTAAAAAGAA+TGG	+	chr4.2:35491875-35491894	None:intergenic	25.0%
!	TTCTGAATTTCAAATGCTTT+AGG	+	chr4.2:35491810-35491829	None:intergenic	25.0%
!	TTCTGATGATAATAGGTAAA+CGG	+	chr4.2:35491907-35491926	None:intergenic	25.0%
!!	CTTTTTAACACATACAACAA+TGG	-	chr4.2:35491877-35491896	MS.gene062963:intron	25.0%
	AAGAAAATTTCTTCTCTGCA+AGG	+	chr4.2:35492109-35492128	None:intergenic	30.0%
	ATGTAAGTGAGAAAATGAAG+CGG	-	chr4.2:35491978-35491997	MS.gene062963:intron	30.0%
	GTGTTGTAGTAGATAGATAA+TGG	+	chr4.2:35491609-35491628	None:intergenic	30.0%
	GTTTACCTATTATCATCAGA+AGG	-	chr4.2:35491906-35491925	MS.gene062963:intron	30.0%
!	GTTTGGTTCAAATATGATAC+AGG	+	chr4.2:35492013-35492032	None:intergenic	30.0%
	AAAAGTTGTGGATGTTCCAA+AGG	-	chr4.2:35491940-35491959	MS.gene062963:intron	35.0%
	AAAGTTGTGGATGTTCCAAA+GGG	-	chr4.2:35491941-35491960	MS.gene062963:intron	35.0%
	ACAACTGCAAACATGTAGAA+TGG	+	chr4.2:35491390-35491409	None:intergenic	35.0%
	ACAATTCTTGGAATGAGGTT+TGG	+	chr4.2:35492030-35492049	None:intergenic	35.0%
	AGAGCATATGAAAGATCACA+TGG	+	chr4.2:35491652-35491671	None:intergenic	35.0%
	ATCTTTCATATGCTCTTGTC+TGG	-	chr4.2:35491656-35491675	MS.gene062963:intron	35.0%
	ATGGTTTCTGTTGATATCCA+TGG	+	chr4.2:35491371-35491390	None:intergenic	35.0%
	ATTCAAATTGTTCCTCAGCT+TGG	+	chr4.2:35492057-35492076	None:intergenic	35.0%
	GACATCATCTTCAAAAGTTG+TGG	-	chr4.2:35491928-35491947	MS.gene062963:intron	35.0%
	GATGTCCTTCTGATGATAAT+AGG	+	chr4.2:35491914-35491933	None:intergenic	35.0%
	GTAACTACAACGATCAATGA+AGG	+	chr4.2:35491706-35491725	None:intergenic	35.0%
!!	CAAATGCTTTAGGATGTGAA+TGG	+	chr4.2:35491800-35491819	None:intergenic	35.0%
	ACTACAACGATCAATGAAGG+AGG	+	chr4.2:35491703-35491722	None:intergenic	40.0%
	ATTTGAATACGACCCTCCAA+TGG	-	chr4.2:35492069-35492088	MS.gene062963:intron	40.0%
	CTACAACGATCAATGAAGGA+GGG	+	chr4.2:35491702-35491721	None:intergenic	40.0%
	GCTCAACAATTCTTGGAATG+AGG	+	chr4.2:35492035-35492054	None:intergenic	40.0%
	TACACTGCTAGATATCCCTT+TGG	+	chr4.2:35491959-35491978	None:intergenic	40.0%
	TGGCCATGACTAAATTCTCA+TGG	-	chr4.2:35491676-35491695	MS.gene062963:intron	40.0%
	TTATGGCTGCAAAAGCAACT+AGG	-	chr4.2:35491762-35491781	MS.gene062963:intron	40.0%
	TTTGAATACGACCCTCCAAT+GGG	-	chr4.2:35492070-35492089	MS.gene062963:intron	40.0%
	AGAATTGTTGAGCCAAGCTG+AGG	-	chr4.2:35492042-35492061	MS.gene062963:intron	45.0%
	CAGCTTGGCTCAACAATTCT+TGG	+	chr4.2:35492042-35492061	None:intergenic	45.0%
	CCAGCTGGTAAGTGACTATA+AGG	-	chr4.2:35491345-35491364	MS.gene062963:CDS	45.0%
	CCTTATAGTCACTTACCAGC+TGG	+	chr4.2:35491348-35491367	None:intergenic	45.0%
	GGGCCATGAGAATTTAGTCA+TGG	+	chr4.2:35491682-35491701	None:intergenic	45.0%
	GGTAAGTGACTATAAGGCCA+TGG	-	chr4.2:35491351-35491370	MS.gene062963:CDS	45.0%
!	TGATAATGCTGATGTCCAGC+TGG	-	chr4.2:35491330-35491349	MS.gene062963:CDS	45.0%
	ATAGTGAGACCACCCATTGG+AGG	+	chr4.2:35492085-35492104	None:intergenic	50.0%
	GGAATAGTGAGACCACCCAT+TGG	+	chr4.2:35492088-35492107	None:intergenic	50.0%
	TAGTGAGACCACCCATTGGA+GGG	+	chr4.2:35492084-35492103	None:intergenic	50.0%
	GAATACGACCCTCCAATGGG+TGG	-	chr4.2:35492073-35492092	MS.gene062963:intron	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	35491304	35492153	35491304	ID=MS.gene062963
chr4.2	mRNA	35491304	35492153	35491304	ID=MS.gene062963.t1;Parent=MS.gene062963
chr4.2	exon	35492106	35492153	35492106	ID=MS.gene062963.t1.exon1;Parent=MS.gene062963.t1
chr4.2	CDS	35492106	35492153	35492106	ID=cds.MS.gene062963.t1;Parent=MS.gene062963.t1
chr4.2	exon	35491955	35491993	35491955	ID=MS.gene062963.t1.exon2;Parent=MS.gene062963.t1
chr4.2	CDS	35491955	35491993	35491955	ID=cds.MS.gene062963.t1;Parent=MS.gene062963.t1
chr4.2	exon	35491304	35491591	35491304	ID=MS.gene062963.t1.exon3;Parent=MS.gene062963.t1
chr4.2	CDS	35491304	35491591	35491304	ID=cds.MS.gene062963.t1;Parent=MS.gene062963.t1

Gene Sequence

>MS.gene062963

ATGCTTCAGACATGTTTTCTGCATTATGATAATGCTGATGTCCAGCTGGTAAGTGACTATAAGGCCATGGATATCAACAGAAACCATTCTACATGTTTGCAGTTGTACTTTCAAGTCTATCAATTATATGATGCTTCATAATTTTGTAAACATGCTTCAGACATGTTTTCTGCATTATGATAATGCTGATGTCCAGCTGGTAAGTGACTATAAGGCCATGGATATCAACAGAAACCATTCTACATGTTTGCAGTTGTACTTTCAAGTCTATCAATTATATGATGCTACTGCTCAAGAATTATCCATTATCTATCTACTACAACACTTGAAGTGTCAAAGACAACGCCATGTGATCTTTCATATGCTCTTGTCTGGCCATGACTAAATTCTCATGGCCCTCCTTCATTGATCGTTGTAGTTACCGACTGTTTGTTACATATCTTTTCATCTATATATATTTATGGCTGCAAAAGCAACTAGGACAAACAAAAAACCATTCACATCCTAAAGCATTTGAAATTCAGAAACTTGAATCTTCTATATACAACTCTTTCTTTTTTAGACCAAACCATTCTTTTTAACACATACAACAATGGGATTCCGTTTACCTATTATCATCAGAAGGACATCATCTTCAAAAGTTGTGGATGTTCCAAAGGGATATCTAGCAGTGTATGTAAGTGAGAAAATGAAGCGGTTTGTGATTCCTGTATCATATTTGAACCAAACCTCATTCCAAGAATTGTTGAGCCAAGCTGAGGAACAATTTGAATACGACCCTCCAATGGGTGGTCTCACTATTCCTTGCAGAGAAGAAATTTTCTTAGATATCACTTCTCGCTTGAATTGA

Protein sequence

>MS.gene062963.t1

MLQTCFLHYDNADVQLTCFLHYDNADVQLTKPFFLTHTTMGFRLPIIIRRTSSSKVVDVPKGYLAVYVSEKMKRFVIPVSYLNQTSFQELLSQAEEQFEYDPPMGGLTIPCREEIFLDITSRLN