AlfalfaGEDB | MS.gene77523

M. sativa cultivar XinJiangDaYe / MS.gene77523

KEGG: K14488
GO: GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
PFAM: PF02519.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77523.t1	XP_003601667.1	95.6	90	4	0	1	90	1	90	5.50E-42	179.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77523.t1	P33080	73.3	90	24	0	1	90	1	90	2.8e-34	145.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77523.t1	G7JAQ1	95.6	90	4	0	1	90	1	90	4.0e-42	179.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene77523	MS.gene062958	PPI
MS.gene77523	MS.gene003628	PPI
MS.gene77523	MS.gene057798	PPI
MS.gene77523	MS.gene057794	PPI
MS.gene77523	MS.gene003629	PPI
MS.gene77523	MS.gene062983	PPI
MS.gene77523	MS.gene062966	PPI
MS.gene77523	MS.gene23367	PPI
MS.gene77523	MS.gene062940	PPI
MS.gene77523	MS.gene003595	PPI
MS.gene062970	MS.gene77523	PPI
MS.gene77523	MS.gene062959	PPI
MS.gene062942	MS.gene77523	PPI
MS.gene77523	MS.gene50664	PPI
MS.gene77523	MS.gene057785	PPI
MS.gene77523	MS.gene50773	PPI
MS.gene77523	MS.gene50670	PPI
MS.gene77523	MS.gene057800	PPI
MS.gene77523	MS.gene50653	PPI
MS.gene77523	MS.gene003598	PPI
MS.gene77523	MS.gene50690	PPI
MS.gene062972	MS.gene77523	PPI
MS.gene77523	MS.gene062941	PPI
MS.gene003580	MS.gene77523	PPI
MS.gene003582	MS.gene77523	PPI
MS.gene77523	MS.gene003613	PPI
MS.gene77523	MS.gene057799	PPI
MS.gene77523	MS.gene50688	PPI
MS.gene77523	MS.gene003603	PPI
MS.gene77523	MS.gene003630	PPI
MS.gene50772	MS.gene77523	PPI
MS.gene77523	MS.gene057779	PPI
MS.gene77523	MS.gene062980	PPI
MS.gene77523	MS.gene50673	PPI
MS.gene057809	MS.gene77523	PPI
MS.gene77523	MS.gene003610	PPI
MS.gene77523	MS.gene50771	PPI
MS.gene062978	MS.gene77523	PPI
MS.gene77523	MS.gene022358	PPI
MS.gene77523	MS.gene057802	PPI
MS.gene77523	MS.gene057796	PPI
MS.gene77523	MS.gene062954	PPI
MS.gene77523	MS.gene50682	PPI
MS.gene77523	MS.gene003597	PPI
MS.gene003593	MS.gene77523	PPI
MS.gene77523	MS.gene057806	PPI
MS.gene057807	MS.gene77523	PPI
MS.gene77523	MS.gene057780	PPI
MS.gene77523	MS.gene057788	PPI
MS.gene77523	MS.gene70684	PPI
MS.gene77523	MS.gene062944	PPI
MS.gene77523	MS.gene50658	PPI
MS.gene77523	MS.gene50655	PPI
MS.gene77523	MS.gene28863	PPI
MS.gene77523	MS.gene50685	PPI
MS.gene77523	MS.gene003584	PPI
MS.gene77523	MS.gene057791	PPI
MS.gene77523	MS.gene50674	PPI
MS.gene77523	MS.gene50651	PPI
MS.gene057816	MS.gene77523	PPI
MS.gene77523	MS.gene057782	PPI
MS.gene77523	MS.gene062969	PPI
MS.gene77523	MS.gene062965	PPI
MS.gene77523	MS.gene50666	PPI
MS.gene77523	MS.gene057784	PPI
MS.gene77523	MS.gene062953	PPI
MS.gene50657	MS.gene77523	PPI
MS.gene77523	MS.gene23369	PPI
MS.gene77523	MS.gene062986	PPI
MS.gene77523	MS.gene057783	PPI
MS.gene77523	MS.gene50770	PPI
MS.gene77523	MS.gene062987	PPI
MS.gene77523	MS.gene057811	PPI
MS.gene50683	MS.gene77523	PPI
MS.gene77523	MS.gene057817	PPI
MS.gene77523	MS.gene062964	PPI
MS.gene77523	MS.gene003612	PPI
MS.gene77523	MS.gene77525	PPI
MS.gene77523	MS.gene057777	PPI
MS.gene77523	MS.gene062945	PPI
MS.gene77523	MS.gene062967	PPI
MS.gene003626	MS.gene77523	PPI
MS.gene77523	MS.gene057804	PPI
MS.gene77523	MS.gene003579	PPI
MS.gene77523	MS.gene062989	PPI
MS.gene062946	MS.gene77523	PPI
MS.gene77523	MS.gene003607	PPI
MS.gene77523	MS.gene50656	PPI
MS.gene057805	MS.gene77523	PPI
MS.gene77523	MS.gene003606	PPI
MS.gene003605	MS.gene77523	PPI
MS.gene77523	MS.gene50686	PPI
MS.gene77523	MS.gene70683	PPI
MS.gene77523	MS.gene062962	PPI
MS.gene77523	MS.gene062985	PPI
MS.gene062971	MS.gene77523	PPI
MS.gene23373	MS.gene77523	PPI
MS.gene77523	MS.gene50669	PPI
MS.gene77523	MS.gene70685	PPI
MS.gene77523	MS.gene003622	PPI
MS.gene062975	MS.gene77523	PPI
MS.gene77523	MS.gene003599	PPI
MS.gene77523	MS.gene23368	PPI
MS.gene77523	MS.gene50662	PPI
MS.gene50689	MS.gene77523	PPI
MS.gene77523	MS.gene003585	PPI
MS.gene77523	MS.gene50660	PPI
MS.gene77523	MS.gene003623	PPI
MS.gene77523	MS.gene50668	PPI
MS.gene77523	MS.gene003581	PPI
MS.gene003576	MS.gene77523	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77523.t1	MTR_3g084150	95.556	90	4	0	1	90	1	90	2.90e-62	184
MS.gene77523.t1	MTR_3g084180	94.444	90	5	0	1	90	1	90	1.98e-61	181
MS.gene77523.t1	MTR_8g461410	92.222	90	7	0	1	90	1	90	1.05e-60	180
MS.gene77523.t1	MTR_3g110042	90.805	87	8	0	1	87	1	87	7.07e-56	167
MS.gene77523.t1	MTR_3g110038	86.813	91	10	2	1	90	1	90	1.04e-53	162
MS.gene77523.t1	MTR_3g084170	86.813	91	10	2	1	90	1	90	1.38e-53	162
MS.gene77523.t1	MTR_3g084240	85.714	91	11	2	1	90	1	90	1.11e-52	159
MS.gene77523.t1	MTR_3g117630	88.095	84	10	0	7	90	7	90	3.81e-52	158
MS.gene77523.t1	MTR_3g084220	84.615	91	12	2	1	90	1	90	6.04e-52	157
MS.gene77523.t1	MTR_3g110032	81.319	91	16	1	1	90	1	91	3.56e-51	155
MS.gene77523.t1	MTR_4g050700	80.220	91	17	1	1	90	1	91	7.12e-51	155
MS.gene77523.t1	MTR_2g012410	80.220	91	17	1	1	90	1	91	7.12e-51	155
MS.gene77523.t1	MTR_3g084260	79.121	91	18	1	1	90	1	91	6.69e-49	150
MS.gene77523.t1	MTR_4g072880	75.824	91	21	1	1	90	1	91	5.63e-48	147
MS.gene77523.t1	MTR_3g084250	73.333	90	24	0	1	90	1	90	1.13e-46	144
MS.gene77523.t1	MTR_4g072250	76.923	91	19	2	1	90	1	90	1.75e-46	144
MS.gene77523.t1	MTR_4g072500	74.444	90	23	0	1	90	1	90	1.83e-46	144
MS.gene77523.t1	MTR_4g072310	73.626	91	23	1	1	90	1	91	2.48e-46	143
MS.gene77523.t1	MTR_4g072330	72.527	91	23	1	1	91	1	89	3.18e-46	143
MS.gene77523.t1	MTR_4g072370	74.725	91	21	1	1	91	1	89	3.40e-46	143
MS.gene77523.t1	MTR_4g072610	74.444	90	17	1	1	90	1	84	9.77e-46	142
MS.gene77523.t1	MTR_4g072570	75.556	90	22	0	1	90	1	90	9.91e-46	142
MS.gene77523.t1	MTR_4g072530	73.333	90	24	0	1	90	1	90	1.51e-45	141
MS.gene77523.t1	MTR_4g072640	74.444	90	23	0	1	90	1	90	1.96e-45	141
MS.gene77523.t1	MTR_4g072270	71.429	91	25	1	1	90	1	91	8.30e-45	139
MS.gene77523.t1	MTR_4g072480	72.222	90	25	0	1	90	1	90	8.89e-45	139
MS.gene77523.t1	MTR_4g072870	69.231	91	27	1	1	90	1	91	9.63e-45	139
MS.gene77523.t1	MTR_4g072730	72.222	90	25	0	1	90	1	90	1.03e-44	139
MS.gene77523.t1	MTR_4g072740	73.333	90	19	1	1	90	1	85	1.16e-44	139
MS.gene77523.t1	MTR_4g072240	71.429	91	25	1	1	90	1	91	1.23e-44	139
MS.gene77523.t1	MTR_4g072680	72.222	90	25	0	1	90	1	90	1.49e-44	139
MS.gene77523.t1	MTR_4g072490	73.626	91	17	2	1	90	1	85	1.98e-44	138
MS.gene77523.t1	MTR_4g072620	71.111	90	26	0	1	90	1	90	2.41e-44	138
MS.gene77523.t1	MTR_4g072230	75.824	91	19	2	1	90	1	89	2.47e-44	138
MS.gene77523.t1	MTR_4g072830	75.281	89	20	1	1	89	1	87	3.02e-44	138
MS.gene77523.t1	MTR_4g072300	71.429	91	25	1	1	90	1	91	5.05e-44	137
MS.gene77523.t1	MTR_4g072860	72.527	91	24	1	1	90	1	91	5.98e-44	137
MS.gene77523.t1	MTR_4g072470	72.222	90	20	1	1	90	1	85	7.90e-44	137
MS.gene77523.t1	MTR_4g072580	73.626	91	17	2	1	90	1	85	1.34e-43	136
MS.gene77523.t1	MTR_4g072650	72.222	90	19	1	1	90	1	84	1.47e-43	136
MS.gene77523.t1	MTR_4g072465	70.000	90	23	1	1	90	1	86	4.36e-43	135
MS.gene77523.t1	MTR_4g072460	70.000	90	27	0	1	90	1	90	5.44e-43	135
MS.gene77523.t1	MTR_4g072220	69.231	91	27	1	1	90	1	91	7.97e-43	134
MS.gene77523.t1	MTR_4g072820	72.527	91	18	2	1	90	1	85	9.82e-43	134
MS.gene77523.t1	MTR_4g072260	67.033	91	29	1	1	90	1	91	1.35e-42	134
MS.gene77523.t1	MTR_4g072890	67.033	91	29	1	1	90	1	91	4.02e-42	133
MS.gene77523.t1	MTR_4g072630	68.889	90	24	1	1	90	1	86	4.67e-42	132
MS.gene77523.t1	MTR_4g072850	73.913	92	22	1	1	90	1	92	3.17e-41	130
MS.gene77523.t1	MTR_4g072540	73.333	90	20	1	1	90	1	86	3.59e-41	130
MS.gene77523.t1	MTR_3g084210	72.222	90	25	0	1	90	1	90	6.64e-41	130
MS.gene77523.t1	MTR_4g072670	71.111	90	22	1	1	90	1	86	6.78e-41	129
MS.gene77523.t1	MTR_4g072720	71.111	90	22	1	1	90	1	86	4.33e-40	127
MS.gene77523.t1	MTR_3g084190	71.111	90	26	0	1	90	1	90	5.22e-40	127
MS.gene77523.t1	MTR_8g461420	71.111	90	26	0	1	90	1	90	7.75e-40	127
MS.gene77523.t1	MTR_4g072600	72.727	88	17	2	1	87	1	82	8.62e-40	127
MS.gene77523.t1	MTR_3g084200	84.932	73	9	2	1	72	1	72	1.33e-39	127
MS.gene77523.t1	MTR_3g084230	71.111	90	26	0	1	90	1	90	1.80e-39	126
MS.gene77523.t1	MTR_5g008360	76.623	77	18	0	14	90	5	81	2.68e-39	125
MS.gene77523.t1	MTR_3g084160	70.000	90	27	0	1	90	1	90	3.16e-39	125
MS.gene77523.t1	MTR_4g072660	66.667	90	25	1	1	90	1	85	4.00e-39	125
MS.gene77523.t1	MTR_4g072510	70.000	90	23	1	1	90	1	86	4.48e-39	125
MS.gene77523.t1	MTR_4g072590	71.111	90	21	1	1	90	1	85	5.42e-39	125
MS.gene77523.t1	MTR_4g072380	68.817	93	23	2	1	92	1	88	8.34e-39	124
MS.gene77523.t1	MTR_3g110045	72.727	88	24	0	3	90	62	149	1.48e-38	125
MS.gene77523.t1	MTR_5g008370	66.667	90	30	0	1	90	1	90	5.24e-37	121
MS.gene77523.t1	MTR_4g072940	72.222	90	23	1	3	90	33	122	6.97e-37	120
MS.gene77523.t1	MTR_4g072690	70.000	90	22	1	1	90	1	85	1.24e-36	119
MS.gene77523.t1	MTR_4g072450	68.889	90	23	1	1	90	1	85	6.62e-36	117
MS.gene77523.t1	MTR_5g008350	71.250	80	16	2	1	79	1	74	7.73e-36	116
MS.gene77523.t1	MTR_4g072980	58.696	92	35	2	1	89	1	92	4.33e-34	112
MS.gene77523.t1	MTR_4g072550	70.423	71	21	0	20	90	31	101	6.50e-33	110
MS.gene77523.t1	MTR_4g072560	51.515	99	40	3	1	91	1	99	4.34e-32	107
MS.gene77523.t1	MTR_4g072770	58.889	90	27	1	1	90	1	80	1.30e-31	106
MS.gene77523.t1	MTR_4g072910	59.036	83	34	0	8	90	18	100	1.67e-31	106
MS.gene77523.t1	MTR_3g117640	67.647	68	21	1	24	90	33	100	3.08e-30	103
MS.gene77523.t1	MTR_4g072750	68.919	74	21	1	1	72	1	74	6.18e-30	102
MS.gene77523.t1	MTR_4g072290	57.143	91	24	2	1	90	1	77	3.11e-28	97.4
MS.gene77523.t1	MTR_4g072280	62.667	75	27	1	1	74	1	75	4.54e-27	94.7
MS.gene77523.t1	MTR_8g461400	57.143	91	11	3	1	90	1	64	6.55e-27	94.0
MS.gene77523.t1	MTR_4g072930	47.917	96	44	2	3	92	17	112	7.25e-26	92.8
MS.gene77523.t1	MTR_3g110035	64.062	64	23	0	27	90	73	136	1.93e-25	92.4
MS.gene77523.t1	MTR_3g113310	58.462	65	26	1	23	87	40	103	1.76e-24	89.0
MS.gene77523.t1	MTR_3g117610	60.000	65	25	1	23	87	43	106	1.52e-23	86.7
MS.gene77523.t1	MTR_6g077850	55.224	67	30	0	24	90	33	99	1.60e-23	86.3
MS.gene77523.t1	MTR_4g072190	51.250	80	36	2	8	87	21	97	4.13e-23	85.1
MS.gene77523.t1	MTR_4g072840	52.273	88	10	3	1	87	1	57	1.57e-22	82.8
MS.gene77523.t1	MTR_5g021820	54.688	64	28	1	24	87	39	101	7.29e-20	77.0
MS.gene77523.t1	MTR_8g076040	50.000	74	36	1	14	87	30	102	8.34e-20	77.0
MS.gene77523.t1	MTR_2g043960	53.030	66	30	1	17	82	68	132	7.08e-19	76.3
MS.gene77523.t1	MTR_4g124750	53.125	64	29	1	24	87	68	130	4.59e-18	73.9
MS.gene77523.t1	MTR_1g009150	52.113	71	33	1	15	85	61	130	5.84e-18	74.3
MS.gene77523.t1	MTR_4g005310	50.000	66	32	1	21	85	58	123	5.30e-17	71.2
MS.gene77523.t1	MTR_4g005320	54.098	61	27	1	25	85	70	129	6.02e-17	71.2
MS.gene77523.t1	MTR_1g094750	44.318	88	45	2	1	85	23	109	1.13e-16	69.3
MS.gene77523.t1	MTR_3g105900	83.333	36	6	0	24	59	1171	1206	2.73e-15	69.7
MS.gene77523.t1	MTR_1g024085	49.254	67	33	1	16	82	28	93	3.49e-15	66.2
MS.gene77523.t1	MTR_4g072160	50.000	70	32	2	17	85	29	96	5.43e-15	65.5
MS.gene77523.t1	MTR_3g109160	55.738	61	26	1	25	85	69	128	6.00e-15	66.2
MS.gene77523.t1	MTR_8g461210	43.038	79	44	1	7	85	17	94	1.60e-14	63.9
MS.gene77523.t1	MTR_7g118010	50.000	64	29	2	24	85	22	84	2.29e-14	63.2
MS.gene77523.t1	MTR_3g092220	41.667	84	43	4	1	82	12	91	3.23e-14	63.2
MS.gene77523.t1	MTR_4g025830	44.776	67	33	2	25	88	79	144	3.39e-14	64.3
MS.gene77523.t1	MTR_0388s0020	41.975	81	42	3	10	87	61	139	6.39e-14	63.2
MS.gene77523.t1	MTR_1g061570	44.156	77	39	2	9	85	6	78	7.19e-14	62.4
MS.gene77523.t1	MTR_7g101740	46.875	64	33	1	24	87	39	101	1.02e-13	61.6
MS.gene77523.t1	MTR_7g405740	40.476	84	45	3	6	85	44	126	2.75e-13	61.6
MS.gene77523.t1	MTR_1g063950	40.580	69	40	1	17	85	40	107	3.83e-13	60.5
MS.gene77523.t1	MTR_8g101520	37.778	90	51	2	7	92	28	116	4.43e-13	61.2
MS.gene77523.t1	MTR_8g026730	48.333	60	28	2	25	82	27	85	5.76e-13	59.7
MS.gene77523.t1	MTR_4g021810	52.542	59	26	2	25	82	26	83	2.17e-12	58.2
MS.gene77523.t1	MTR_3g098970	35.135	74	47	1	17	90	45	117	3.94e-12	58.5
MS.gene77523.t1	MTR_1g059770	31.034	87	59	1	6	92	30	115	4.75e-12	57.8
MS.gene77523.t1	MTR_3g097120	38.750	80	40	2	19	90	3	81	1.81e-11	55.8
MS.gene77523.t1	MTR_8g096470	41.667	60	34	1	26	85	47	105	5.11e-11	54.7
MS.gene77523.t1	MTR_2g066830	37.313	67	41	1	25	91	58	123	6.94e-11	55.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77523.t1	AT4G38840	59.596	99	31	2	1	90	1	99	2.15e-36	119
MS.gene77523.t1	AT5G18020	67.105	76	24	1	16	90	15	90	1.32e-34	114
MS.gene77523.t1	AT5G18060	68.919	74	21	2	19	90	17	90	3.12e-33	110
MS.gene77523.t1	AT4G38825	65.333	75	25	1	17	90	15	89	8.03e-33	109
MS.gene77523.t1	AT5G18050	63.158	76	27	1	16	90	15	90	8.45e-33	109
MS.gene77523.t1	AT5G18080	64.474	76	26	1	16	90	15	90	1.01e-32	109
MS.gene77523.t1	AT5G18030	68.056	72	22	1	20	90	17	88	1.02e-32	109
MS.gene77523.t1	AT2G21200	66.667	72	23	1	19	89	15	86	5.98e-32	107
MS.gene77523.t1	AT2G21210	63.514	74	25	1	19	90	22	95	9.94e-32	107
MS.gene77523.t1	AT5G18010	61.842	76	28	1	16	90	15	90	2.26e-31	105
MS.gene77523.t1	AT2G21210	63.514	74	25	1	19	90	89	162	6.35e-31	107
MS.gene77523.t1	AT4G34770	68.056	72	21	1	18	87	28	99	4.33e-30	103
MS.gene77523.t1	AT4G34800	56.322	87	34	2	7	89	2	88	1.48e-29	101
MS.gene77523.t1	AT3G03840	61.111	72	26	1	21	90	23	94	6.44e-29	99.8
MS.gene77523.t1	AT4G38850	56.098	82	31	2	12	88	6	87	2.36e-28	98.2
MS.gene77523.t1	AT3G03850	67.164	67	20	1	26	90	26	92	5.94e-28	97.4
MS.gene77523.t1	AT4G34810	61.765	68	22	1	24	87	47	114	6.86e-28	97.8
MS.gene77523.t1	AT3G03820	56.579	76	30	1	18	90	20	95	2.59e-27	95.9
MS.gene77523.t1	AT3G03830	51.648	91	37	2	7	90	2	92	9.41e-27	94.4
MS.gene77523.t1	AT4G13790	50.602	83	34	2	8	88	13	90	1.83e-25	90.9
MS.gene77523.t1	AT4G34780	58.904	73	27	1	23	92	26	98	2.03e-25	91.3
MS.gene77523.t1	AT4G34790	57.143	70	28	1	25	92	39	108	6.98e-24	87.4
MS.gene77523.t1	AT1G75580	51.899	79	37	1	9	87	28	105	1.24e-23	87.0
MS.gene77523.t1	AT4G38860	56.923	65	28	0	23	87	38	102	1.72e-23	86.3
MS.gene77523.t1	AT4G34760	46.429	84	44	1	4	87	22	104	3.90e-22	83.2
MS.gene77523.t1	AT2G21220	56.923	65	27	1	23	87	38	101	4.38e-22	82.8
MS.gene77523.t1	AT4G36110	47.619	84	41	2	8	89	21	103	4.06e-21	80.5
MS.gene77523.t1	AT2G16580	49.315	73	36	1	15	87	34	105	4.26e-20	77.8
MS.gene77523.t1	AT3G43120	50.725	69	33	1	14	82	68	135	5.61e-20	79.0
MS.gene77523.t1	AT2G18010	53.030	66	30	1	24	89	47	111	6.28e-20	77.8
MS.gene77523.t1	AT3G20220	48.684	76	37	2	15	89	37	111	1.11e-19	77.0
MS.gene77523.t1	AT1G19830	45.977	87	34	2	8	82	19	104	5.71e-19	75.5
MS.gene77523.t1	AT5G20810	53.030	66	30	1	17	82	71	135	2.70e-18	74.7
MS.gene77523.t1	AT5G20810	53.030	66	30	1	17	82	71	135	2.97e-18	75.5
MS.gene77523.t1	AT5G66260	47.674	86	40	3	8	90	15	98	1.22e-16	68.9
MS.gene77523.t1	AT1G75590	54.237	59	26	1	24	82	41	98	6.18e-16	68.6
MS.gene77523.t1	AT5G10990	47.945	73	31	2	24	90	40	111	2.28e-15	67.0
MS.gene77523.t1	AT4G31320	48.101	79	33	3	8	85	68	139	2.81e-15	67.4
MS.gene77523.t1	AT1G19840	54.237	59	26	1	24	82	40	97	8.15e-15	65.5
MS.gene77523.t1	AT4G34750	37.975	79	48	1	9	87	21	98	1.02e-14	65.1
MS.gene77523.t1	AT4G34750	37.975	79	48	1	9	87	21	98	1.02e-14	65.1
MS.gene77523.t1	AT3G51200	46.269	67	34	2	20	85	34	99	2.40e-14	63.2
MS.gene77523.t1	AT2G24400	49.367	79	32	3	8	85	52	123	3.17e-14	64.7
MS.gene77523.t1	AT2G37030	46.032	63	33	1	24	86	48	109	1.28e-13	62.0
MS.gene77523.t1	AT3G61900	44.286	70	35	2	24	90	29	97	4.00e-13	60.8
MS.gene77523.t1	AT2G46690	43.478	69	35	2	17	82	16	83	4.41e-13	60.5
MS.gene77523.t1	AT3G12830	36.667	90	52	2	6	91	27	115	9.08e-13	59.7
MS.gene77523.t1	AT4G00880	52.459	61	26	2	24	82	27	86	2.73e-12	58.5
MS.gene77523.t1	AT3G53250	42.857	63	32	1	24	86	51	109	3.14e-12	58.2
MS.gene77523.t1	AT3G09870	47.692	65	31	2	15	77	35	98	4.53e-12	57.4
MS.gene77523.t1	AT1G56150	43.284	67	37	1	25	91	38	103	4.67e-12	57.4
MS.gene77523.t1	AT3G53250	42.857	63	32	1	24	86	82	140	5.87e-12	58.2
MS.gene77523.t1	AT4G09530	41.667	72	37	3	14	82	19	88	1.51e-11	55.8
MS.gene77523.t1	AT5G53590	41.791	67	33	2	24	85	45	110	1.74e-11	56.6
MS.gene77523.t1	AT4G12410	42.647	68	35	2	25	89	76	142	2.00e-11	57.0
MS.gene77523.t1	AT1G16510	38.961	77	45	2	16	91	44	119	4.52e-11	55.8

Find 20 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATTAGACGAACTTCATTTAC+AGG	0.268008	3.2:-72557957	MS.gene77523:CDS
TTCGTCTAATAGCAGGTAAA+CGG	0.451949	3.2:+72557970	None:intergenic
AGTCAAGCAGAGGAAGAATT+CGG	0.474154	3.2:-72557807	MS.gene77523:CDS
GGCTATCTTGCAGTATATGT+TGG	0.486267	3.2:-72557897	MS.gene77523:CDS
GAATTGTGAGGCCACCCATT+GGG	0.504738	3.2:+72557775	None:intergenic
AATGAAGTTCGTCTAATAGC+AGG	0.529225	3.2:+72557963	None:intergenic
TTCGGATATGATCACCCAAT+GGG	0.534324	3.2:-72557789	MS.gene77523:CDS
GGAATTGTGAGGCCACCCAT+TGG	0.541668	3.2:+72557774	None:intergenic
TATACTGCAAGATAGCCCTT+TGG	0.576450	3.2:+72557903	None:intergenic
ATGAAAACATCTTCTGTGCA+AGG	0.577322	3.2:+72557753	None:intergenic
AAAGCTGTGAATGTGCCAAA+GGG	0.584403	3.2:-72557918	MS.gene77523:CDS
AAAAGCTGTGAATGTGCCAA+AGG	0.591032	3.2:-72557919	MS.gene77523:CDS
ATTCGGATATGATCACCCAA+TGG	0.609742	3.2:-72557790	MS.gene77523:CDS
AGCTTGGTTCAAGTATGACA+TGG	0.610689	3.2:+72557848	None:intergenic
GCTTGGTTCAAGTATGACAT+GGG	0.618074	3.2:+72557849	None:intergenic
AAATTTATTGAGTCAAGCAG+AGG	0.625368	3.2:-72557817	MS.gene77523:CDS
GGATATGATCACCCAATGGG+TGG	0.648309	3.2:-72557786	MS.gene77523:CDS
CTTGGTTCAAGTATGACATG+GGG	0.689184	3.2:+72557850	None:intergenic
ATGTTGGAGAGCAAATGAAG+CGG	0.701417	3.2:-72557881	MS.gene77523:CDS
CTTCTGTGCAAGGAATTGTG+AGG	0.738315	3.2:+72557763	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATAAATTTTGAAATGAAGCT+TGG	+	chr3.2:72557871-72557890	None:intergenic	20.0%
	AAATTTATTGAGTCAAGCAG+AGG	-	chr3.2:72557883-72557902	MS.gene77523:CDS	30.0%
	ATTAGACGAACTTCATTTAC+AGG	-	chr3.2:72557743-72557762	MS.gene77523:CDS	30.0%
	AATGAAGTTCGTCTAATAGC+AGG	+	chr3.2:72557740-72557759	None:intergenic	35.0%
	ATGAAAACATCTTCTGTGCA+AGG	+	chr3.2:72557950-72557969	None:intergenic	35.0%
	TTCGTCTAATAGCAGGTAAA+CGG	+	chr3.2:72557733-72557752	None:intergenic	35.0%
	AAAAGCTGTGAATGTGCCAA+AGG	-	chr3.2:72557781-72557800	MS.gene77523:CDS	40.0%
	AAAGCTGTGAATGTGCCAAA+GGG	-	chr3.2:72557782-72557801	MS.gene77523:CDS	40.0%
	AGCTTGGTTCAAGTATGACA+TGG	+	chr3.2:72557855-72557874	None:intergenic	40.0%
	AGTCAAGCAGAGGAAGAATT+CGG	-	chr3.2:72557893-72557912	MS.gene77523:CDS	40.0%
	ATGTTGGAGAGCAAATGAAG+CGG	-	chr3.2:72557819-72557838	MS.gene77523:CDS	40.0%
	ATTCGGATATGATCACCCAA+TGG	-	chr3.2:72557910-72557929	MS.gene77523:CDS	40.0%
	CTTGGTTCAAGTATGACATG+GGG	+	chr3.2:72557853-72557872	None:intergenic	40.0%
	GCTTGGTTCAAGTATGACAT+GGG	+	chr3.2:72557854-72557873	None:intergenic	40.0%
	GGCTATCTTGCAGTATATGT+TGG	-	chr3.2:72557803-72557822	MS.gene77523:CDS	40.0%
	TATACTGCAAGATAGCCCTT+TGG	+	chr3.2:72557800-72557819	None:intergenic	40.0%
	TTCGGATATGATCACCCAAT+GGG	-	chr3.2:72557911-72557930	MS.gene77523:CDS	40.0%
!!!	TCACAGCTTTTGATGATGCT+TGG	+	chr3.2:72557772-72557791	None:intergenic	40.0%
	CTTCTGTGCAAGGAATTGTG+AGG	+	chr3.2:72557940-72557959	None:intergenic	45.0%
	GAATTGTGAGGCCACCCATT+GGG	+	chr3.2:72557928-72557947	None:intergenic	50.0%
	GGATATGATCACCCAATGGG+TGG	-	chr3.2:72557914-72557933	MS.gene77523:CDS	50.0%
	GGAATTGTGAGGCCACCCAT+TGG	+	chr3.2:72557929-72557948	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	72557722	72558000	72557722	ID=MS.gene77523
chr3.2	mRNA	72557722	72558000	72557722	ID=MS.gene77523.t1;Parent=MS.gene77523
chr3.2	exon	72557722	72558000	72557722	ID=MS.gene77523.t1.exon1;Parent=MS.gene77523.t1
chr3.2	CDS	72557722	72558000	72557722	ID=cds.MS.gene77523.t1;Parent=MS.gene77523.t1

Gene Sequence	>MS.gene77523 ATGGGTTTCCGTTTACCTGCTATTAGACGAACTTCATTTACAGGTAGCCAAGCATCATCAAAAGCTGTGAATGTGCCAAAGGGCTATCTTGCAGTATATGTTGGAGAGCAAATGAAGCGGTTTGTTATCCCCATGTCATACTTGAACCAAGCTTCATTTCAAAATTTATTGAGTCAAGCAGAGGAAGAATTCGGATATGATCACCCAATGGGTGGCCTCACAATTCCTTGCACAGAAGATGTTTTCATTCAAATTACTTCTCGCTTCAATTTAAACTAA
Protein sequence	>MS.gene77523.t1 MGFRLPAIRRTSFTGSQASSKAVNVPKGYLAVYVGEQMKRFVIPMSYLNQASFQNLLSQAEEEFGYDHPMGGLTIPCTEDVFIQITSRFNLN