AlfalfaGEDB | MS.gene003598

M. sativa cultivar XinJiangDaYe / MS.gene003598

KEGG: K14488
GO: GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
PFAM: PF02519.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene003598.t1	AES89321.1	90.7	86	8	0	1	86	1	86	2.60E-38	167.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene003598.t1	P33080	66.7	90	26	1	1	86	1	90	3.9e-30	131.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene003598.t1	G7JL46	90.7	86	8	0	1	86	1	86	1.9e-38	167.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene003598	MS.gene50652	PPI
MS.gene003598	MS.gene014669	PPI
MS.gene003598	MS.gene057808	PPI
MS.gene003598	MS.gene002380	PPI
MS.gene23372	MS.gene003598	PPI
MS.gene50659	MS.gene003598	PPI
MS.gene75316	MS.gene003598	PPI
MS.gene057790	MS.gene003598	PPI
MS.gene003624	MS.gene003598	PPI
MS.gene23371	MS.gene003598	PPI
MS.gene003598	MS.gene68163	PPI
MS.gene031201	MS.gene003598	PPI
MS.gene003598	MS.gene75315	PPI
MS.gene003598	MS.gene68164	PPI
MS.gene003598	MS.gene68759	PPI
MS.gene75676	MS.gene003598	PPI
MS.gene003598	MS.gene79889	PPI
MS.gene045362	MS.gene003598	PPI
MS.gene003598	MS.gene014222	PPI
MS.gene003598	MS.gene79888	PPI
MS.gene77523	MS.gene003598	PPI
MS.gene003598	MS.gene75678	PPI
MS.gene003598	MS.gene014223	PPI
MS.gene003598	MS.gene79885	PPI
MS.gene75674	MS.gene003598	PPI
MS.gene79890	MS.gene003598	PPI
MS.gene003615	MS.gene003598	PPI
MS.gene062973	MS.gene003598	PPI
MS.gene003602	MS.gene003598	PPI
MS.gene047517	MS.gene003598	PPI
MS.gene003627	MS.gene003598	PPI
MS.gene003577	MS.gene003598	PPI
MS.gene062984	MS.gene003598	PPI
MS.gene50687	MS.gene003598	PPI
MS.gene40327	MS.gene003598	PPI
MS.gene003591	MS.gene003598	PPI
MS.gene003587	MS.gene003598	PPI
MS.gene003598	MS.gene75314	PPI
MS.gene003598	MS.gene01764	PPI
MS.gene003590	MS.gene003598	PPI
MS.gene045368	MS.gene003598	PPI
MS.gene062943	MS.gene003598	PPI
MS.gene063112	MS.gene003598	PPI
MS.gene003598	MS.gene75679	PPI
MS.gene003608	MS.gene003598	PPI
MS.gene77526	MS.gene003598	PPI
MS.gene003598	MS.gene031198	PPI
MS.gene045360	MS.gene003598	PPI
MS.gene003598	MS.gene003614	PPI
MS.gene003598	MS.gene50764	PPI
MS.gene003598	MS.gene77528	PPI
MS.gene003598	MS.gene014671	PPI
MS.gene057818	MS.gene003598	PPI
MS.gene003598	MS.gene003596	PPI
MS.gene50766	MS.gene003598	PPI
MS.gene003598	MS.gene75672	PPI
MS.gene75677	MS.gene003598	PPI
MS.gene50677	MS.gene003598	PPI
MS.gene003625	MS.gene003598	PPI
MS.gene003598	MS.gene50654	PPI
MS.gene003598	MS.gene062974	PPI
MS.gene057795	MS.gene003598	PPI
MS.gene003598	MS.gene014670	PPI
MS.gene68165	MS.gene003598	PPI
MS.gene003598	MS.gene062951	PPI
MS.gene003598	MS.gene057801	PPI
MS.gene003598	MS.gene77524	PPI
MS.gene045361	MS.gene003598	PPI
MS.gene003598	MS.gene68162	PPI
MS.gene50675	MS.gene003598	PPI
MS.gene003598	MS.gene75675	PPI
MS.gene003598	MS.gene79887	PPI
MS.gene062979	MS.gene003598	PPI
MS.gene01358	MS.gene003598	PPI
MS.gene003598	MS.gene40325	PPI
MS.gene40326	MS.gene003598	PPI
MS.gene062952	MS.gene003598	PPI
MS.gene003598	MS.gene50684	PPI
MS.gene003598	MS.gene057797	PPI
MS.gene031199	MS.gene003598	PPI
MS.gene003598	MS.gene014668	PPI
MS.gene50676	MS.gene003598	PPI
MS.gene003598	MS.gene79886	PPI
MS.gene003598	MS.gene50765	PPI
MS.gene23370	MS.gene003598	PPI
MS.gene77529	MS.gene003598	PPI
MS.gene68761	MS.gene003598	PPI
MS.gene014667	MS.gene003598	PPI
MS.gene68760	MS.gene003598	PPI
MS.gene003594	MS.gene003598	PPI
MS.gene50665	MS.gene003598	PPI
MS.gene003586	MS.gene003598	PPI
MS.gene057778	MS.gene003598	PPI
MS.gene062988	MS.gene003598	PPI
MS.gene75673	MS.gene003598	PPI
MS.gene003598	MS.gene003583	PPI
MS.gene045366	MS.gene003598	PPI
MS.gene003598	MS.gene77527	PPI
MS.gene003598	MS.gene79891	PPI
MS.gene003598	MS.gene045367	PPI
MS.gene062963	MS.gene003598	PPI
MS.gene003598	MS.gene045364	PPI
MS.gene003598	MS.gene045363	PPI
MS.gene50763	MS.gene003598	PPI
MS.gene031200	MS.gene003598	PPI
MS.gene045365	MS.gene003598	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene003598.t1	MTR_4g072630	90.698	86	8	0	1	86	1	86	1.38e-56	169
MS.gene003598.t1	MTR_4g072465	87.209	86	11	0	1	86	1	86	4.71e-54	162
MS.gene003598.t1	MTR_4g072490	89.535	86	8	1	1	86	1	85	5.56e-54	162
MS.gene003598.t1	MTR_4g072740	88.372	86	9	1	1	86	1	85	1.02e-52	159
MS.gene003598.t1	MTR_4g072470	86.047	86	11	1	1	86	1	85	4.44e-49	150
MS.gene003598.t1	MTR_4g072510	90.698	86	8	0	1	86	1	86	1.01e-48	149
MS.gene003598.t1	MTR_4g072670	90.698	86	8	0	1	86	1	86	3.80e-48	147
MS.gene003598.t1	MTR_4g072370	80.682	88	15	1	1	86	1	88	5.70e-48	147
MS.gene003598.t1	MTR_4g072580	81.395	86	15	1	1	86	1	85	1.27e-47	146
MS.gene003598.t1	MTR_4g072540	88.372	86	10	0	1	86	1	86	3.34e-47	145
MS.gene003598.t1	MTR_4g072720	88.372	86	10	0	1	86	1	86	3.90e-47	145
MS.gene003598.t1	MTR_4g072830	80.460	87	15	1	1	85	1	87	6.04e-47	144
MS.gene003598.t1	MTR_4g072600	77.907	86	18	1	1	86	1	85	5.74e-45	139
MS.gene003598.t1	MTR_4g072610	79.070	86	16	1	1	86	1	84	1.30e-44	139
MS.gene003598.t1	MTR_3g084170	73.333	90	20	1	1	86	1	90	3.61e-44	137
MS.gene003598.t1	MTR_4g072650	77.907	86	17	1	1	86	1	84	3.89e-44	137
MS.gene003598.t1	MTR_4g072870	70.652	92	20	2	1	86	1	91	2.98e-43	135
MS.gene003598.t1	MTR_4g072330	74.157	89	19	2	1	86	1	88	3.93e-43	135
MS.gene003598.t1	MTR_3g084150	70.000	90	23	1	1	86	1	90	4.03e-43	135
MS.gene003598.t1	MTR_3g084240	72.222	90	21	1	1	86	1	90	4.31e-43	135
MS.gene003598.t1	MTR_3g084220	71.111	90	22	1	1	86	1	90	9.81e-43	134
MS.gene003598.t1	MTR_3g084180	68.889	90	24	1	1	86	1	90	3.54e-42	132
MS.gene003598.t1	MTR_5g008350	82.667	75	12	1	1	75	1	74	4.92e-42	132
MS.gene003598.t1	MTR_8g461410	67.778	90	25	1	1	86	1	90	1.38e-41	131
MS.gene003598.t1	MTR_4g072590	80.233	86	16	1	1	86	1	85	1.70e-41	130
MS.gene003598.t1	MTR_3g110038	70.000	90	23	1	1	86	1	90	3.25e-41	130
MS.gene003598.t1	MTR_3g110032	69.231	91	23	1	1	86	1	91	3.50e-41	130
MS.gene003598.t1	MTR_4g072450	81.395	86	15	1	1	86	1	85	3.88e-41	130
MS.gene003598.t1	MTR_4g072690	79.070	86	17	1	1	86	1	85	4.83e-41	129
MS.gene003598.t1	MTR_4g072240	67.033	91	25	1	1	86	1	91	6.65e-41	129
MS.gene003598.t1	MTR_4g050700	69.231	91	23	1	1	86	1	91	1.70e-40	128
MS.gene003598.t1	MTR_2g012410	69.231	91	23	1	1	86	1	91	1.70e-40	128
MS.gene003598.t1	MTR_4g072310	69.231	91	23	1	1	86	1	91	2.38e-40	128
MS.gene003598.t1	MTR_4g072380	75.862	87	19	2	1	86	1	86	4.36e-40	127
MS.gene003598.t1	MTR_4g072480	66.667	90	26	1	1	86	1	90	7.09e-40	127
MS.gene003598.t1	MTR_4g072300	64.835	91	27	1	1	86	1	91	8.29e-40	127
MS.gene003598.t1	MTR_4g072890	64.835	91	27	1	1	86	1	91	1.36e-39	126
MS.gene003598.t1	MTR_4g072880	65.934	91	26	1	1	86	1	91	1.38e-39	126
MS.gene003598.t1	MTR_3g110042	69.663	89	23	1	1	85	1	89	3.05e-39	125
MS.gene003598.t1	MTR_4g072680	66.667	90	26	1	1	86	1	90	5.16e-39	125
MS.gene003598.t1	MTR_4g072260	63.736	91	28	1	1	86	1	91	6.62e-39	124
MS.gene003598.t1	MTR_4g072250	71.429	91	20	2	1	86	1	90	6.86e-39	124
MS.gene003598.t1	MTR_4g072220	63.736	91	28	1	1	86	1	91	7.15e-39	124
MS.gene003598.t1	MTR_3g084260	67.033	91	25	1	1	86	1	91	1.07e-38	124
MS.gene003598.t1	MTR_4g072620	63.333	90	29	1	1	86	1	90	2.06e-38	123
MS.gene003598.t1	MTR_4g072850	67.391	92	24	1	1	86	1	92	3.94e-38	122
MS.gene003598.t1	MTR_4g072570	66.667	90	26	1	1	86	1	90	4.06e-38	122
MS.gene003598.t1	MTR_4g072820	72.414	87	21	2	1	86	1	85	4.73e-38	122
MS.gene003598.t1	MTR_4g072270	65.934	91	26	1	1	86	1	91	5.38e-38	122
MS.gene003598.t1	MTR_3g084250	66.667	90	26	1	1	86	1	90	5.70e-38	122
MS.gene003598.t1	MTR_4g072860	70.652	92	20	2	1	86	1	91	1.29e-37	121
MS.gene003598.t1	MTR_4g072460	64.444	90	28	1	1	86	1	90	1.97e-37	120
MS.gene003598.t1	MTR_4g072500	66.667	90	26	1	1	86	1	90	3.84e-37	120
MS.gene003598.t1	MTR_4g072730	62.222	90	30	1	1	86	1	90	4.02e-37	120
MS.gene003598.t1	MTR_4g072530	65.556	90	27	1	1	86	1	90	1.65e-36	118
MS.gene003598.t1	MTR_3g117630	73.973	73	19	0	14	86	18	90	3.30e-36	117
MS.gene003598.t1	MTR_3g084190	67.778	90	25	1	1	86	1	90	3.41e-36	117
MS.gene003598.t1	MTR_4g072640	65.934	91	25	2	1	86	1	90	3.68e-36	117
MS.gene003598.t1	MTR_3g084210	65.556	90	27	1	1	86	1	90	6.16e-36	117
MS.gene003598.t1	MTR_4g072230	65.934	91	24	2	1	86	1	89	1.64e-35	116
MS.gene003598.t1	MTR_4g072660	62.791	86	31	1	1	86	1	85	1.84e-35	115
MS.gene003598.t1	MTR_8g461420	66.667	90	26	1	1	86	1	90	1.91e-35	115
MS.gene003598.t1	MTR_3g084160	66.667	90	26	1	1	86	1	90	5.46e-35	114
MS.gene003598.t1	MTR_3g110045	68.182	88	24	1	3	86	62	149	7.48e-35	116
MS.gene003598.t1	MTR_3g084230	65.556	90	27	1	1	86	1	90	9.98e-35	114
MS.gene003598.t1	MTR_5g008370	64.444	90	28	1	1	86	1	90	3.24e-34	114
MS.gene003598.t1	MTR_5g008360	66.667	78	26	0	9	86	4	81	3.91e-34	112
MS.gene003598.t1	MTR_4g072940	64.444	90	26	1	3	86	33	122	3.79e-33	111
MS.gene003598.t1	MTR_3g084200	68.056	72	19	1	1	68	1	72	3.94e-31	105
MS.gene003598.t1	MTR_4g072770	59.302	86	29	1	1	86	1	80	5.61e-30	101
MS.gene003598.t1	MTR_4g072560	50.515	97	36	3	1	85	1	97	9.22e-30	101
MS.gene003598.t1	MTR_4g072980	53.261	92	36	2	1	85	1	92	2.97e-29	100
MS.gene003598.t1	MTR_4g072910	61.039	77	29	1	10	86	25	100	9.73e-29	99.4
MS.gene003598.t1	MTR_4g072550	61.972	71	27	0	16	86	31	101	4.87e-28	97.4
MS.gene003598.t1	MTR_3g117640	61.765	68	25	1	20	86	33	100	1.01e-27	96.7
MS.gene003598.t1	MTR_4g072290	58.140	86	27	2	1	86	1	77	2.40e-26	92.4
MS.gene003598.t1	MTR_4g072750	64.384	73	20	2	1	67	1	73	1.90e-24	87.8
MS.gene003598.t1	MTR_4g072280	56.000	75	28	1	1	70	1	75	2.23e-24	87.8
MS.gene003598.t1	MTR_3g110035	60.938	64	25	0	23	86	73	136	4.32e-24	88.6
MS.gene003598.t1	MTR_6g077850	55.224	67	30	0	20	86	33	99	7.87e-23	84.3
MS.gene003598.t1	MTR_3g117610	58.462	65	26	1	19	83	43	106	3.88e-22	82.8
MS.gene003598.t1	MTR_3g113310	53.731	67	30	1	19	85	40	105	1.86e-21	80.9
MS.gene003598.t1	MTR_4g072190	50.685	73	35	1	11	83	26	97	3.93e-21	80.1
MS.gene003598.t1	MTR_4g072930	48.148	81	37	2	11	86	30	110	1.44e-20	79.0
MS.gene003598.t1	MTR_8g461400	48.889	90	16	2	1	86	1	64	1.46e-20	77.4
MS.gene003598.t1	MTR_4g072840	50.575	87	15	3	1	86	1	60	8.72e-19	72.8
MS.gene003598.t1	MTR_5g021820	44.048	84	44	2	4	85	21	103	2.79e-18	72.8
MS.gene003598.t1	MTR_8g076040	51.562	64	30	1	20	83	40	102	1.73e-17	70.9
MS.gene003598.t1	MTR_2g043960	54.237	59	26	1	20	78	75	132	8.89e-17	70.5
MS.gene003598.t1	MTR_4g124750	52.542	59	27	1	20	78	68	125	1.50e-16	69.7
MS.gene003598.t1	MTR_5g008380	85.294	34	5	0	53	86	244	277	3.60e-15	68.2
MS.gene003598.t1	MTR_1g094750	50.000	62	30	1	20	81	49	109	8.45e-15	64.3
MS.gene003598.t1	MTR_1g009150	47.826	69	35	1	13	81	63	130	1.36e-14	65.1
MS.gene003598.t1	MTR_1g061570	45.570	79	40	2	5	81	1	78	1.39e-14	63.9
MS.gene003598.t1	MTR_3g105900	77.778	36	8	0	20	55	1171	1206	3.08e-14	66.2
MS.gene003598.t1	MTR_4g005310	48.387	62	31	1	21	81	62	123	3.33e-14	63.5
MS.gene003598.t1	MTR_7g101740	46.875	64	33	1	20	83	39	101	1.02e-13	61.6
MS.gene003598.t1	MTR_7g118010	46.154	65	32	2	21	83	23	86	1.12e-13	61.2
MS.gene003598.t1	MTR_1g063950	43.548	62	34	1	20	81	47	107	1.78e-13	61.2
MS.gene003598.t1	MTR_1g024085	49.153	59	29	1	20	78	36	93	6.17e-13	60.5
MS.gene003598.t1	MTR_3g092220	42.029	69	37	2	12	78	24	91	6.34e-13	59.3
MS.gene003598.t1	MTR_4g005320	45.902	61	32	1	21	81	70	129	6.88e-13	60.5
MS.gene003598.t1	MTR_4g072160	42.667	75	41	2	8	81	23	96	3.44e-12	58.2
MS.gene003598.t1	MTR_8g461210	40.506	79	42	2	4	78	14	91	4.79e-12	57.4
MS.gene003598.t1	MTR_3g109160	49.180	61	30	1	21	81	69	128	5.17e-12	58.5
MS.gene003598.t1	MTR_8g026730	41.270	63	34	2	21	81	27	88	2.45e-11	55.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene003598.t1	AT4G38840	69.118	68	20	1	20	86	32	99	3.35e-32	108
MS.gene003598.t1	AT4G34800	57.143	84	33	1	1	81	1	84	4.07e-30	102
MS.gene003598.t1	AT5G18080	60.811	74	28	1	14	86	17	90	1.07e-29	101
MS.gene003598.t1	AT2G21210	51.852	81	37	1	8	86	15	95	3.69e-29	100
MS.gene003598.t1	AT5G18020	59.459	74	29	1	14	86	17	90	8.76e-29	99.4
MS.gene003598.t1	AT5G18050	58.974	78	28	2	10	86	16	90	9.40e-29	99.0
MS.gene003598.t1	AT5G18060	65.152	66	22	1	22	86	25	90	1.20e-28	99.0
MS.gene003598.t1	AT4G38825	58.974	78	26	2	10	86	17	89	1.30e-28	98.6
MS.gene003598.t1	AT4G34810	61.538	78	26	1	10	83	37	114	1.41e-28	99.4
MS.gene003598.t1	AT5G18030	58.228	79	29	2	9	86	13	88	1.75e-28	98.2
MS.gene003598.t1	AT4G38850	57.317	82	27	2	8	86	13	89	2.24e-28	98.2
MS.gene003598.t1	AT2G21210	51.852	81	37	1	8	86	82	162	2.39e-28	100
MS.gene003598.t1	AT5G18010	58.108	74	30	1	14	86	17	90	5.67e-28	97.1
MS.gene003598.t1	AT2G21200	58.333	72	29	1	15	85	15	86	2.02e-27	95.5
MS.gene003598.t1	AT3G03840	55.128	78	33	1	11	86	17	94	1.20e-25	91.3
MS.gene003598.t1	AT3G03820	53.165	79	34	1	11	86	17	95	1.95e-25	90.9
MS.gene003598.t1	AT4G34770	54.762	84	36	1	5	86	19	102	5.16e-25	90.1
MS.gene003598.t1	AT3G03850	61.194	67	24	1	22	86	26	92	7.51e-25	89.4
MS.gene003598.t1	AT4G13790	47.059	85	42	2	3	85	8	91	3.20e-24	87.8
MS.gene003598.t1	AT3G03830	55.696	79	29	2	11	86	17	92	8.39e-24	86.7
MS.gene003598.t1	AT4G34790	64.179	67	21	2	21	84	39	105	1.50e-23	86.3
MS.gene003598.t1	AT4G38860	48.235	85	42	1	4	86	21	105	9.54e-23	84.3
MS.gene003598.t1	AT2G21220	47.561	82	40	2	4	83	21	101	4.95e-21	80.1
MS.gene003598.t1	AT4G34780	55.714	70	28	1	19	85	26	95	6.42e-21	79.7
MS.gene003598.t1	AT1G75580	56.716	67	28	1	19	85	42	107	1.12e-20	79.3
MS.gene003598.t1	AT4G34760	55.385	65	28	1	19	83	41	104	5.18e-20	77.4
MS.gene003598.t1	AT2G16580	53.846	65	29	1	19	83	42	105	2.50e-19	75.9
MS.gene003598.t1	AT4G36110	51.562	64	30	1	20	83	39	101	8.79e-18	71.6
MS.gene003598.t1	AT1G19830	56.667	60	25	1	19	78	46	104	1.13e-17	71.6
MS.gene003598.t1	AT3G43120	52.542	59	27	1	20	78	78	135	2.92e-17	71.6
MS.gene003598.t1	AT2G18010	50.000	64	31	1	20	83	47	109	4.98e-17	70.1
MS.gene003598.t1	AT5G20810	54.237	59	26	1	20	78	78	135	1.52e-16	70.1
MS.gene003598.t1	AT5G20810	54.237	59	26	1	20	78	78	135	2.43e-16	70.1
MS.gene003598.t1	AT3G20220	51.613	62	28	2	21	81	47	107	1.30e-14	63.9
MS.gene003598.t1	AT5G66260	50.000	66	32	1	21	86	34	98	1.99e-14	63.2
MS.gene003598.t1	AT2G37030	47.619	63	32	1	20	82	48	109	1.50e-13	61.2
MS.gene003598.t1	AT3G51200	43.902	82	40	3	5	81	19	99	2.86e-13	60.1
MS.gene003598.t1	AT1G75590	47.458	59	30	1	20	78	41	98	9.02e-13	60.1
MS.gene003598.t1	AT5G10990	42.667	75	37	2	4	78	29	97	1.62e-12	59.3
MS.gene003598.t1	AT4G34750	40.625	64	37	1	20	83	36	98	1.64e-12	59.3
MS.gene003598.t1	AT4G34750	40.625	64	37	1	20	83	36	98	1.64e-12	59.3
MS.gene003598.t1	AT2G46690	40.260	77	42	2	5	78	8	83	3.59e-12	57.8
MS.gene003598.t1	AT1G19840	44.000	75	36	2	4	78	29	97	4.94e-12	58.2
MS.gene003598.t1	AT3G53250	42.857	63	32	1	20	82	51	109	6.35e-12	57.4
MS.gene003598.t1	AT3G53250	42.857	63	32	1	20	82	82	140	1.36e-11	57.0
MS.gene003598.t1	AT4G31320	45.902	61	32	1	21	81	80	139	2.83e-11	56.6
MS.gene003598.t1	AT4G00880	41.026	78	40	3	3	78	13	86	6.17e-11	54.7
MS.gene003598.t1	AT2G24400	47.541	61	31	1	21	81	64	123	6.33e-11	55.8

Find 21 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CACTTTGGCTGAACAATTCT+TGG	0.170812	4.1:-30877356	None:intergenic
GGCATCATCTTCAAAAGGTT+TGG	0.355226	4.1:+30877245	MS.gene003598:CDS
GTTTGGTTCAAGTATGATAT+AGG	0.375326	4.1:-30877327	None:intergenic
ACAATTCTTGGAATGATGTT+TGG	0.420414	4.1:-30877344	None:intergenic
ATTCGAATTGTTCCTCACTT+TGG	0.475430	4.1:-30877371	None:intergenic
TCAAGGGCATCATCTTCAAA+AGG	0.490872	4.1:+30877240	MS.gene003598:CDS
TACCAAGTATCATCAGATCA+AGG	0.490956	4.1:+30877223	MS.gene003598:CDS
AAAAGGTTTGGATGTGCCAA+AGG	0.498708	4.1:+30877257	MS.gene003598:CDS
TTCGAATATGATCATCCAAT+GGG	0.511444	4.1:+30877387	MS.gene003598:CDS
GCCCTTGATCTGATGATACT+TGG	0.512708	4.1:-30877225	None:intergenic
TACACTGCTAGATAGCCCTT+TGG	0.516931	4.1:-30877273	None:intergenic
ATTCGAATATGATCATCCAA+TGG	0.544520	4.1:+30877386	MS.gene003598:CDS
AAAGGTTTGGATGTGCCAAA+GGG	0.563932	4.1:+30877258	MS.gene003598:CDS
AAGAAAACTTCTTCTCTGCA+AGG	0.584904	4.1:-30877423	None:intergenic
ACCAAGTATCATCAGATCAA+GGG	0.600732	4.1:+30877224	MS.gene003598:CDS
GGAATAGTGAGACCACCCAT+TGG	0.603922	4.1:-30877402	None:intergenic
AGAATTGTTCAGCCAAAGTG+AGG	0.630997	4.1:+30877359	MS.gene003598:CDS
CTAAATTCAATTCAAGTGAG+AGG	0.633235	4.1:-30877454	None:intergenic
GAATATGATCATCCAATGGG+TGG	0.654072	4.1:+30877390	MS.gene003598:CDS
GGCTATCTAGCAGTGTACGT+AGG	0.714634	4.1:+30877279	MS.gene003598:CDS
ACGTAGGAGAGAAAATGAAG+CGG	0.744637	4.1:+30877295	MS.gene003598:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ACAATTCTTGGAATGATGTT+TGG	-	chr4.1:30877347-30877366	None:intergenic	30.0%
	ATTCGAATATGATCATCCAA+TGG	+	chr4.1:30877386-30877405	MS.gene003598:CDS	30.0%
	TTCGAATATGATCATCCAAT+GGG	+	chr4.1:30877387-30877406	MS.gene003598:CDS	30.0%
!	GTTTGGTTCAAGTATGATAT+AGG	-	chr4.1:30877330-30877349	None:intergenic	30.0%
	AAGAAAACTTCTTCTCTGCA+AGG	-	chr4.1:30877426-30877445	None:intergenic	35.0%
	ACCAAGTATCATCAGATCAA+GGG	+	chr4.1:30877224-30877243	MS.gene003598:CDS	35.0%
	ATTCGAATTGTTCCTCACTT+TGG	-	chr4.1:30877374-30877393	None:intergenic	35.0%
	TACCAAGTATCATCAGATCA+AGG	+	chr4.1:30877223-30877242	MS.gene003598:CDS	35.0%
	AAAAGGTTTGGATGTGCCAA+AGG	+	chr4.1:30877257-30877276	MS.gene003598:CDS	40.0%
	AAAGGTTTGGATGTGCCAAA+GGG	+	chr4.1:30877258-30877277	MS.gene003598:CDS	40.0%
	ACGTAGGAGAGAAAATGAAG+CGG	+	chr4.1:30877295-30877314	MS.gene003598:CDS	40.0%
	AGAATTGTTCAGCCAAAGTG+AGG	+	chr4.1:30877359-30877378	MS.gene003598:CDS	40.0%
	CACTTTGGCTGAACAATTCT+TGG	-	chr4.1:30877359-30877378	None:intergenic	40.0%
	GAATATGATCATCCAATGGG+TGG	+	chr4.1:30877390-30877409	MS.gene003598:CDS	40.0%
	GGCATCATCTTCAAAAGGTT+TGG	+	chr4.1:30877245-30877264	MS.gene003598:CDS	40.0%
!!	TCAAGGGCATCATCTTCAAA+AGG	+	chr4.1:30877240-30877259	MS.gene003598:CDS	40.0%
	GCCCTTGATCTGATGATACT+TGG	-	chr4.1:30877228-30877247	None:intergenic	45.0%
	TACACTGCTAGATAGCCCTT+TGG	-	chr4.1:30877276-30877295	None:intergenic	45.0%
	GGAATAGTGAGACCACCCAT+TGG	-	chr4.1:30877405-30877424	None:intergenic	50.0%
	GGCTATCTAGCAGTGTACGT+AGG	+	chr4.1:30877279-30877298	MS.gene003598:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	30877210	30877470	30877210	ID=MS.gene003598
chr4.1	mRNA	30877210	30877470	30877210	ID=MS.gene003598.t1;Parent=MS.gene003598
chr4.1	exon	30877210	30877470	30877210	ID=MS.gene003598.t1.exon1;Parent=MS.gene003598.t1
chr4.1	CDS	30877210	30877470	30877210	ID=cds.MS.gene003598.t1;Parent=MS.gene003598.t1

Gene Sequence	>MS.gene003598 ATGGGTTTTCGTTTACCAAGTATCATCAGATCAAGGGCATCATCTTCAAAAGGTTTGGATGTGCCAAAGGGCTATCTAGCAGTGTACGTAGGAGAGAAAATGAAGCGGTTTGTGATTCCTATATCATACTTGAACCAAACATCATTCCAAGAATTGTTCAGCCAAAGTGAGGAACAATTCGAATATGATCATCCAATGGGTGGTCTCACTATTCCTTGCAGAGAAGAAGTTTTCTTAGATATCACCTCTCACTTGAATTGA
Protein sequence	>MS.gene003598.t1 MGFRLPSIIRSRASSSKGLDVPKGYLAVYVGEKMKRFVIPISYLNQTSFQELFSQSEEQFEYDHPMGGLTIPCREEVFLDITSHLN