Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66847.t1 | XP_003604015.1 | 97.5 | 162 | 4 | 0 | 1 | 162 | 62 | 223 | 1.10E-64 | 256.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66847.t1 | O65693 | 67.3 | 162 | 39 | 1 | 1 | 162 | 62 | 209 | 2.4e-49 | 196.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66847.t1 | G7J7U0 | 97.5 | 162 | 4 | 0 | 1 | 162 | 62 | 223 | 7.6e-65 | 256.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049134 | MS.gene66847 | -0.806228 | 9.11E-50 | -1.69E-46 |
| MS.gene049149 | MS.gene66847 | 0.829694 | 4.46E-55 | -1.69E-46 |
| MS.gene049150 | MS.gene66847 | 0.833982 | 3.90E-56 | -1.69E-46 |
| MS.gene049153 | MS.gene66847 | 0.829647 | 4.58E-55 | -1.69E-46 |
| MS.gene049284 | MS.gene66847 | 0.818259 | 2.15E-52 | -1.69E-46 |
| MS.gene049297 | MS.gene66847 | 0.813185 | 2.91E-51 | -1.69E-46 |
| MS.gene049346 | MS.gene66847 | 0.84477 | 6.16E-59 | -1.69E-46 |
| MS.gene049351 | MS.gene66847 | 0.824003 | 1.02E-53 | -1.69E-46 |
| MS.gene049426 | MS.gene66847 | 0.809379 | 1.95E-50 | -1.69E-46 |
| MS.gene049491 | MS.gene66847 | 0.829732 | 4.37E-55 | -1.69E-46 |
| MS.gene049661 | MS.gene66847 | 0.844494 | 7.30E-59 | -1.69E-46 |
| MS.gene049662 | MS.gene66847 | 0.842188 | 3.01E-58 | -1.69E-46 |
| MS.gene049758 | MS.gene66847 | 0.854882 | 9.16E-62 | -1.69E-46 |
| MS.gene049910 | MS.gene66847 | 0.876636 | 1.19E-68 | -1.69E-46 |
| MS.gene049915 | MS.gene66847 | 0.819485 | 1.13E-52 | -1.69E-46 |
| MS.gene049993 | MS.gene66847 | 0.822477 | 2.32E-53 | -1.69E-46 |
| MS.gene050076 | MS.gene66847 | 0.802074 | 6.67E-49 | -1.69E-46 |
| MS.gene050128 | MS.gene66847 | 0.802567 | 5.28E-49 | -1.69E-46 |
| MS.gene050227 | MS.gene66847 | 0.804218 | 2.40E-49 | -1.69E-46 |
| MS.gene050231 | MS.gene66847 | 0.818749 | 1.67E-52 | -1.69E-46 |
| MS.gene050301 | MS.gene66847 | 0.896125 | 4.98E-76 | -1.69E-46 |
| MS.gene050330 | MS.gene66847 | -0.809233 | 2.09E-50 | -1.69E-46 |
| MS.gene050397 | MS.gene66847 | 0.858792 | 6.46E-63 | -1.69E-46 |
| MS.gene050404 | MS.gene66847 | 0.807508 | 4.88E-50 | -1.69E-46 |
| MS.gene050406 | MS.gene66847 | 0.847055 | 1.47E-59 | -1.69E-46 |
| MS.gene050426 | MS.gene66847 | -0.835093 | 2.05E-56 | -1.69E-46 |
| MS.gene050464 | MS.gene66847 | 0.88312 | 5.87E-71 | -1.69E-46 |
| MS.gene050529 | MS.gene66847 | 0.871676 | 5.70E-67 | -1.69E-46 |
| MS.gene050558 | MS.gene66847 | 0.865333 | 6.37E-65 | -1.69E-46 |
| MS.gene050560 | MS.gene66847 | 0.825459 | 4.63E-54 | -1.69E-46 |
| MS.gene050625 | MS.gene66847 | 0.806932 | 6.47E-50 | -1.69E-46 |
| MS.gene050642 | MS.gene66847 | 0.830853 | 2.32E-55 | -1.69E-46 |
| MS.gene050650 | MS.gene66847 | 0.825214 | 5.30E-54 | -1.69E-46 |
| MS.gene050670 | MS.gene66847 | 0.867406 | 1.40E-65 | -1.69E-46 |
| MS.gene050787 | MS.gene66847 | 0.816914 | 4.33E-52 | -1.69E-46 |
| MS.gene050820 | MS.gene66847 | 0.828742 | 7.59E-55 | -1.69E-46 |
| MS.gene05101 | MS.gene66847 | 0.865352 | 6.29E-65 | -1.69E-46 |
| MS.gene05105 | MS.gene66847 | 0.837014 | 6.67E-57 | -1.69E-46 |
| MS.gene05119 | MS.gene66847 | -0.802715 | 4.92E-49 | -1.69E-46 |
| MS.gene051471 | MS.gene66847 | 0.830625 | 2.64E-55 | -1.69E-46 |
| MS.gene051525 | MS.gene66847 | 0.85455 | 1.14E-61 | -1.69E-46 |
| MS.gene051582 | MS.gene66847 | 0.849991 | 2.27E-60 | -1.69E-46 |
| MS.gene051663 | MS.gene66847 | 0.806835 | 6.78E-50 | -1.69E-46 |
| MS.gene051860 | MS.gene66847 | 0.889836 | 1.69E-73 | -1.69E-46 |
| MS.gene051983 | MS.gene66847 | 0.837846 | 4.08E-57 | -1.69E-46 |
| MS.gene052011 | MS.gene66847 | -0.867256 | 1.57E-65 | -1.69E-46 |
| MS.gene052077 | MS.gene66847 | 0.869452 | 3.06E-66 | -1.69E-46 |
| MS.gene052078 | MS.gene66847 | 0.840141 | 1.04E-57 | -1.69E-46 |
| MS.gene052178 | MS.gene66847 | 0.846495 | 2.10E-59 | -1.69E-46 |
| MS.gene052188 | MS.gene66847 | 0.870185 | 1.77E-66 | -1.69E-46 |
| MS.gene052207 | MS.gene66847 | 0.820755 | 5.80E-53 | -1.69E-46 |
| MS.gene052253 | MS.gene66847 | 0.905 | 6.81E-80 | -1.69E-46 |
| MS.gene052255 | MS.gene66847 | 0.823659 | 1.23E-53 | -1.69E-46 |
| MS.gene052310 | MS.gene66847 | 0.869982 | 2.06E-66 | -1.69E-46 |
| MS.gene052322 | MS.gene66847 | 0.821765 | 3.39E-53 | -1.69E-46 |
| MS.gene052340 | MS.gene66847 | 0.880541 | 5.04E-70 | -1.69E-46 |
| MS.gene052481 | MS.gene66847 | 0.863461 | 2.45E-64 | -1.69E-46 |
| MS.gene052485 | MS.gene66847 | 0.847554 | 1.07E-59 | -1.69E-46 |
| MS.gene052917 | MS.gene66847 | -0.813345 | 2.68E-51 | -1.69E-46 |
| MS.gene053103 | MS.gene66847 | 0.806073 | 9.82E-50 | -1.69E-46 |
| MS.gene053209 | MS.gene66847 | 0.828744 | 7.58E-55 | -1.69E-46 |
| MS.gene053557 | MS.gene66847 | 0.860051 | 2.70E-63 | -1.69E-46 |
| MS.gene053836 | MS.gene66847 | 0.906913 | 8.92E-81 | -1.69E-46 |
| MS.gene053971 | MS.gene66847 | 0.809705 | 1.66E-50 | -1.69E-46 |
| MS.gene054010 | MS.gene66847 | 0.832712 | 8.08E-56 | -1.69E-46 |
| MS.gene054011 | MS.gene66847 | 0.854203 | 1.44E-61 | -1.69E-46 |
| MS.gene054012 | MS.gene66847 | 0.839031 | 2.02E-57 | -1.69E-46 |
| MS.gene054013 | MS.gene66847 | 0.831984 | 1.22E-55 | -1.69E-46 |
| MS.gene054123 | MS.gene66847 | 0.815398 | 9.44E-52 | -1.69E-46 |
| MS.gene054183 | MS.gene66847 | 0.87283 | 2.35E-67 | -1.69E-46 |
| MS.gene054184 | MS.gene66847 | 0.804148 | 2.48E-49 | -1.69E-46 |
| MS.gene054185 | MS.gene66847 | 0.807281 | 5.46E-50 | -1.69E-46 |
| MS.gene054349 | MS.gene66847 | 0.82774 | 1.32E-54 | -1.69E-46 |
| MS.gene054473 | MS.gene66847 | 0.868629 | 5.67E-66 | -1.69E-46 |
| MS.gene054542 | MS.gene66847 | 0.812053 | 5.14E-51 | -1.69E-46 |
| MS.gene05455 | MS.gene66847 | 0.816565 | 5.18E-52 | -1.69E-46 |
| MS.gene054719 | MS.gene66847 | 0.821338 | 4.26E-53 | -1.69E-46 |
| MS.gene054917 | MS.gene66847 | 0.815379 | 9.53E-52 | -1.69E-46 |
| MS.gene054918 | MS.gene66847 | 0.826768 | 2.26E-54 | -1.69E-46 |
| MS.gene054930 | MS.gene66847 | 0.821681 | 3.55E-53 | -1.69E-46 |
| MS.gene054955 | MS.gene66847 | 0.8051 | 1.57E-49 | -1.69E-46 |
| MS.gene054983 | MS.gene66847 | 0.862016 | 6.83E-64 | -1.69E-46 |
| MS.gene055116 | MS.gene66847 | 0.894358 | 2.66E-75 | -1.69E-46 |
| MS.gene055277 | MS.gene66847 | 0.864027 | 1.63E-64 | -1.69E-46 |
| MS.gene055358 | MS.gene66847 | 0.801509 | 8.71E-49 | -1.69E-46 |
| MS.gene055390 | MS.gene66847 | 0.821146 | 4.71E-53 | -1.69E-46 |
| MS.gene055422 | MS.gene66847 | 0.879588 | 1.10E-69 | -1.69E-46 |
| MS.gene055428 | MS.gene66847 | 0.872276 | 3.60E-67 | -1.69E-46 |
| MS.gene05553 | MS.gene66847 | 0.840699 | 7.43E-58 | -1.69E-46 |
| MS.gene055601 | MS.gene66847 | -0.816131 | 6.48E-52 | -1.69E-46 |
| MS.gene055604 | MS.gene66847 | 0.841889 | 3.61E-58 | -1.69E-46 |
| MS.gene056191 | MS.gene66847 | 0.868114 | 8.31E-66 | -1.69E-46 |
| MS.gene056407 | MS.gene66847 | 0.837151 | 6.16E-57 | -1.69E-46 |
| MS.gene056451 | MS.gene66847 | 0.826157 | 3.16E-54 | -1.69E-46 |
| MS.gene056511 | MS.gene66847 | 0.806614 | 7.55E-50 | -1.69E-46 |
| MS.gene056612 | MS.gene66847 | 0.855909 | 4.60E-62 | -1.69E-46 |
| MS.gene056659 | MS.gene66847 | 0.822272 | 2.59E-53 | -1.69E-46 |
| MS.gene056723 | MS.gene66847 | 0.843164 | 1.66E-58 | -1.69E-46 |
| MS.gene056796 | MS.gene66847 | 0.800617 | 1.33E-48 | -1.69E-46 |
| MS.gene056846 | MS.gene66847 | 0.807016 | 6.21E-50 | -1.69E-46 |
| MS.gene056899 | MS.gene66847 | -0.862707 | 4.19E-64 | -1.69E-46 |
| MS.gene056979 | MS.gene66847 | 0.836501 | 9.02E-57 | -1.69E-46 |
| MS.gene05702 | MS.gene66847 | 0.875116 | 3.97E-68 | -1.69E-46 |
| MS.gene057121 | MS.gene66847 | 0.869966 | 2.08E-66 | -1.69E-46 |
| MS.gene057123 | MS.gene66847 | 0.811584 | 6.51E-51 | -1.69E-46 |
| MS.gene057124 | MS.gene66847 | 0.807395 | 5.16E-50 | -1.69E-46 |
| MS.gene057146 | MS.gene66847 | 0.808709 | 2.71E-50 | -1.69E-46 |
| MS.gene057245 | MS.gene66847 | 0.860109 | 2.60E-63 | -1.69E-46 |
| MS.gene057310 | MS.gene66847 | 0.804021 | 2.64E-49 | -1.69E-46 |
| MS.gene057330 | MS.gene66847 | 0.807517 | 4.86E-50 | -1.69E-46 |
| MS.gene05733 | MS.gene66847 | 0.828238 | 1.00E-54 | -1.69E-46 |
| MS.gene057396 | MS.gene66847 | 0.818386 | 2.01E-52 | -1.69E-46 |
| MS.gene057439 | MS.gene66847 | 0.819525 | 1.11E-52 | -1.69E-46 |
| MS.gene057448 | MS.gene66847 | 0.836108 | 1.14E-56 | -1.69E-46 |
| MS.gene057449 | MS.gene66847 | 0.826881 | 2.13E-54 | -1.69E-46 |
| MS.gene057450 | MS.gene66847 | 0.828991 | 6.61E-55 | -1.69E-46 |
| MS.gene057469 | MS.gene66847 | 0.82385 | 1.11E-53 | -1.69E-46 |
| MS.gene057476 | MS.gene66847 | 0.830506 | 2.83E-55 | -1.69E-46 |
| MS.gene057749 | MS.gene66847 | 0.849916 | 2.38E-60 | -1.69E-46 |
| MS.gene057779 | MS.gene66847 | 0.824768 | 6.75E-54 | -1.69E-46 |
| MS.gene057782 | MS.gene66847 | 0.81421 | 1.73E-51 | -1.69E-46 |
| MS.gene057823 | MS.gene66847 | 0.83587 | 1.30E-56 | -1.69E-46 |
| MS.gene057825 | MS.gene66847 | 0.813186 | 2.91E-51 | -1.69E-46 |
| MS.gene058002 | MS.gene66847 | 0.902521 | 8.91E-79 | -1.69E-46 |
| MS.gene058007 | MS.gene66847 | 0.804126 | 2.51E-49 | -1.69E-46 |
| MS.gene05811 | MS.gene66847 | 0.888592 | 5.15E-73 | -1.69E-46 |
| MS.gene058139 | MS.gene66847 | 0.801715 | 7.90E-49 | -1.69E-46 |
| MS.gene058142 | MS.gene66847 | 0.868233 | 7.60E-66 | -1.69E-46 |
| MS.gene058218 | MS.gene66847 | -0.812503 | 4.10E-51 | -1.69E-46 |
| MS.gene058233 | MS.gene66847 | 0.852862 | 3.49E-61 | -1.69E-46 |
| MS.gene058276 | MS.gene66847 | 0.841871 | 3.65E-58 | -1.69E-46 |
| MS.gene058293 | MS.gene66847 | 0.808804 | 2.59E-50 | -1.69E-46 |
| MS.gene058376 | MS.gene66847 | 0.805067 | 1.60E-49 | -1.69E-46 |
| MS.gene05842 | MS.gene66847 | 0.813282 | 2.77E-51 | -1.69E-46 |
| MS.gene058444 | MS.gene66847 | 0.811696 | 6.15E-51 | -1.69E-46 |
| MS.gene05856 | MS.gene66847 | 0.807135 | 5.86E-50 | -1.69E-46 |
| MS.gene058660 | MS.gene66847 | 0.873916 | 1.01E-67 | -1.69E-46 |
| MS.gene058710 | MS.gene66847 | 0.836675 | 8.14E-57 | -1.69E-46 |
| MS.gene058749 | MS.gene66847 | 0.81758 | 3.06E-52 | -1.69E-46 |
| MS.gene058845 | MS.gene66847 | 0.825099 | 5.64E-54 | -1.69E-46 |
| MS.gene05886 | MS.gene66847 | 0.863218 | 2.91E-64 | -1.69E-46 |
| MS.gene058928 | MS.gene66847 | 0.868647 | 5.59E-66 | -1.69E-46 |
| MS.gene059011 | MS.gene66847 | 0.810082 | 1.38E-50 | -1.69E-46 |
| MS.gene05907 | MS.gene66847 | 0.891259 | 4.68E-74 | -1.69E-46 |
| MS.gene059082 | MS.gene66847 | 0.802956 | 4.39E-49 | -1.69E-46 |
| MS.gene059141 | MS.gene66847 | 0.853576 | 2.18E-61 | -1.69E-46 |
| MS.gene059247 | MS.gene66847 | 0.814195 | 1.74E-51 | -1.69E-46 |
| MS.gene059285 | MS.gene66847 | 0.863887 | 1.81E-64 | -1.69E-46 |
| MS.gene059294 | MS.gene66847 | 0.852848 | 3.53E-61 | -1.69E-46 |
| MS.gene059342 | MS.gene66847 | 0.803091 | 4.11E-49 | -1.69E-46 |
| MS.gene059366 | MS.gene66847 | 0.80681 | 6.87E-50 | -1.69E-46 |
| MS.gene059420 | MS.gene66847 | 0.869362 | 3.28E-66 | -1.69E-46 |
| MS.gene059462 | MS.gene66847 | 0.826174 | 3.14E-54 | -1.69E-46 |
| MS.gene059518 | MS.gene66847 | 0.852336 | 4.94E-61 | -1.69E-46 |
| MS.gene059536 | MS.gene66847 | 0.816894 | 4.37E-52 | -1.69E-46 |
| MS.gene059597 | MS.gene66847 | 0.873743 | 1.16E-67 | -1.69E-46 |
| MS.gene059676 | MS.gene66847 | 0.827978 | 1.16E-54 | -1.69E-46 |
| MS.gene059688 | MS.gene66847 | 0.889207 | 2.98E-73 | -1.69E-46 |
| MS.gene059692 | MS.gene66847 | 0.882902 | 7.05E-71 | -1.69E-46 |
| MS.gene059695 | MS.gene66847 | 0.883396 | 4.65E-71 | -1.69E-46 |
| MS.gene059807 | MS.gene66847 | 0.802884 | 4.54E-49 | -1.69E-46 |
| MS.gene05984 | MS.gene66847 | 0.90682 | 9.86E-81 | -1.69E-46 |
| MS.gene059876 | MS.gene66847 | 0.807536 | 4.82E-50 | -1.69E-46 |
| MS.gene059894 | MS.gene66847 | 0.854681 | 1.05E-61 | -1.69E-46 |
| MS.gene060247 | MS.gene66847 | 0.824832 | 6.52E-54 | -1.69E-46 |
| MS.gene06037 | MS.gene66847 | 0.835303 | 1.82E-56 | -1.69E-46 |
| MS.gene060403 | MS.gene66847 | 0.857571 | 1.49E-62 | -1.69E-46 |
| MS.gene060407 | MS.gene66847 | 0.811784 | 5.89E-51 | -1.69E-46 |
| MS.gene060476 | MS.gene66847 | 0.809037 | 2.31E-50 | -1.69E-46 |
| MS.gene060986 | MS.gene66847 | 0.828111 | 1.08E-54 | -1.69E-46 |
| MS.gene060994 | MS.gene66847 | 0.816802 | 4.58E-52 | -1.69E-46 |
| MS.gene061000 | MS.gene66847 | 0.811257 | 7.67E-51 | -1.69E-46 |
| MS.gene061049 | MS.gene66847 | 0.900182 | 9.46E-78 | -1.69E-46 |
| MS.gene061051 | MS.gene66847 | -0.80475 | 1.86E-49 | -1.69E-46 |
| MS.gene061114 | MS.gene66847 | -0.816352 | 5.78E-52 | -1.69E-46 |
| MS.gene061154 | MS.gene66847 | 0.824886 | 6.33E-54 | -1.69E-46 |
| MS.gene061159 | MS.gene66847 | 0.837045 | 6.55E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66847.t1 | MTR_3g117600 | 98.148 | 162 | 3 | 0 | 1 | 162 | 62 | 223 | 1.99e-112 | 318 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66847.t1 | AT4G34730 | 69.753 | 162 | 35 | 1 | 1 | 162 | 62 | 209 | 4.62e-74 | 221 |
| MS.gene66847.t1 | AT4G34730 | 69.753 | 162 | 35 | 1 | 1 | 162 | 62 | 209 | 4.62e-74 | 221 |
| MS.gene66847.t1 | AT4G34730 | 69.753 | 162 | 35 | 1 | 1 | 162 | 62 | 209 | 4.62e-74 | 221 |
Find 29 sgRNAs with CRISPR-Local
Find 116 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACTCACTGCGAACATATT+TGG | 0.174334 | 3.3:-95479295 | None:intergenic |
| TGAAATCACCGCTGATTTAC+AGG | 0.294810 | 3.3:+95478932 | MS.gene66847:CDS |
| AAATATGTTCGCAGTGAGTT+GGG | 0.366464 | 3.3:+95479297 | MS.gene66847:CDS |
| GGCAAATTGCAAGACGTTAT+CGG | 0.368809 | 3.3:-95478844 | None:intergenic |
| GGGAAGGAAGTGGCCATGGC+TGG | 0.375787 | 3.3:+95479258 | MS.gene66847:CDS |
| GTTCTTCCTGAAGCTTCCTT+AGG | 0.384742 | 3.3:+95478867 | MS.gene66847:CDS |
| AAGTTCTCTCCGAATTTGTT+TGG | 0.408703 | 3.3:-95478805 | None:intergenic |
| TCAGCACCTAAGGAAGCTTC+AGG | 0.415676 | 3.3:-95478873 | None:intergenic |
| ACATAAATGATACCTTCTTC+AGG | 0.417511 | 3.3:-95480476 | None:intergenic |
| AAATGGTTGCCAAACAAATT+CGG | 0.421978 | 3.3:+95478796 | None:intergenic |
| CAGATGAAGACGATGCTGAT+TGG | 0.423415 | 3.3:+95480441 | MS.gene66847:CDS |
| GATTTCAACGTCGGTGATAG+TGG | 0.425429 | 3.3:-95478916 | None:intergenic |
| AGATGAAGACGATGCTGATT+GGG | 0.431019 | 3.3:+95480442 | MS.gene66847:CDS |
| GAGGTAAGAATCAGCACCTA+AGG | 0.457882 | 3.3:-95478883 | None:intergenic |
| CAAATATGTTCGCAGTGAGT+TGG | 0.480055 | 3.3:+95479296 | MS.gene66847:CDS |
| CATAAATGATACCTTCTTCA+GGG | 0.510113 | 3.3:-95480475 | None:intergenic |
| GGAAGGAAGTGGCCATGGCT+GGG | 0.525786 | 3.3:+95479259 | MS.gene66847:CDS |
| AGATGAAAGAGGGAAGGAAG+TGG | 0.566160 | 3.3:+95479248 | MS.gene66847:CDS |
| GAAGATGAAGACCCTGAAGA+AGG | 0.579729 | 3.3:+95480464 | MS.gene66847:CDS |
| AAGAGGGAAGGAAGTGGCCA+TGG | 0.582663 | 3.3:+95479254 | MS.gene66847:CDS |
| TGGGGAGGCGTATGAAGTTG+CGG | 0.591668 | 3.3:+95479316 | MS.gene66847:CDS |
| AATATGTTCGCAGTGAGTTG+GGG | 0.594957 | 3.3:+95479298 | MS.gene66847:CDS |
| TAAGGAAGCTTCAGGAAGAA+CGG | 0.601825 | 3.3:-95478865 | None:intergenic |
| ATGTTCGCAGTGAGTTGGGG+AGG | 0.601873 | 3.3:+95479301 | MS.gene66847:CDS |
| AACGTCGGTGATAGTGGTGA+CGG | 0.620179 | 3.3:-95478910 | None:intergenic |
| TGATAGTGGTGACGGAAGAG+AGG | 0.626472 | 3.3:-95478902 | None:intergenic |
| CAGTTGGATTCATCAGTGAA+CGG | 0.643319 | 3.3:+95480389 | MS.gene66847:CDS |
| ATCAGCGGTGATTTCAACGT+CGG | 0.653697 | 3.3:-95478925 | None:intergenic |
| AATAAGAACCTGTAAATCAG+CGG | 0.693763 | 3.3:-95478940 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACATATAGAAAAAAATATA+AGG | - | chr3.3:95479661-95479680 | None:intergenic | 10.0% |
| !! | ATCATGAAACAAAATAAATA+AGG | - | chr3.3:95479145-95479164 | None:intergenic | 15.0% |
| !! | ATTAAATGAGAAAAATGAAA+AGG | - | chr3.3:95479101-95479120 | None:intergenic | 15.0% |
| !! | TGCTTAAATATATAAAAGAA+TGG | - | chr3.3:95480228-95480247 | None:intergenic | 15.0% |
| !!! | CATATTCGTTAATTTTAAAA+AGG | + | chr3.3:95480091-95480110 | MS.gene66847:intron | 15.0% |
| !! | AAGATAAAAAAAGATTTCAG+CGG | - | chr3.3:95480064-95480083 | None:intergenic | 20.0% |
| !! | GAATTATCACACTATAAATT+CGG | - | chr3.3:95478993-95479012 | None:intergenic | 20.0% |
| !! | TCATCAGGAAAATAAAAATA+AGG | - | chr3.3:95479971-95479990 | None:intergenic | 20.0% |
| !!! | ATATTTACCGTTTCTTTTTA+CGG | + | chr3.3:95479405-95479424 | MS.gene66847:intron | 20.0% |
| ! | AGTGATACATAAGAATAAGA+TGG | + | chr3.3:95479849-95479868 | MS.gene66847:intron | 25.0% |
| ! | CTCATTTAATCTAGTGTATT+TGG | + | chr3.3:95479111-95479130 | MS.gene66847:intron | 25.0% |
| ! | TATATATTTAAGCAGCTTCT+TGG | + | chr3.3:95480234-95480253 | MS.gene66847:intron | 25.0% |
| ! | TCTATCTACCATATATATGT+TGG | + | chr3.3:95479709-95479728 | MS.gene66847:intron | 25.0% |
| ! | TTTATAGTGTGATAATTCAG+TGG | + | chr3.3:95478995-95479014 | MS.gene66847:intron | 25.0% |
| !! | TATTCAAACAATGCTTTTGA+GGG | + | chr3.3:95479915-95479934 | MS.gene66847:intron | 25.0% |
| AAGAACCATCTCATAATTGT+GGG | - | chr3.3:95479780-95479799 | None:intergenic | 30.0% | |
| AATAAGAACCTGTAAATCAG+CGG | - | chr3.3:95478943-95478962 | None:intergenic | 30.0% | |
| AGAAAAATGAAAAGGCAATG+TGG | - | chr3.3:95479093-95479112 | None:intergenic | 30.0% | |
| ATTATGAGATGGTTCTTACT+AGG | + | chr3.3:95479783-95479802 | MS.gene66847:intron | 30.0% | |
| TAAGAACCATCTCATAATTG+TGG | - | chr3.3:95479781-95479800 | None:intergenic | 30.0% | |
| TATCTAATATTGCAATGACC+TGG | - | chr3.3:95480328-95480347 | None:intergenic | 30.0% | |
| TTATCACACTATAAATTCGG+CGG | - | chr3.3:95478990-95479009 | None:intergenic | 30.0% | |
| ! | ATTCAAACAATGCTTTTGAG+GGG | + | chr3.3:95479916-95479935 | MS.gene66847:intron | 30.0% |
| ! | CTATTCAAACAATGCTTTTG+AGG | + | chr3.3:95479914-95479933 | MS.gene66847:intron | 30.0% |
| ! | TAACTCAACACTTTTAGTGT+AGG | + | chr3.3:95479197-95479216 | MS.gene66847:intron | 30.0% |
| ! | TTTCCTGATGATGAAATTGT+GGG | + | chr3.3:95479980-95479999 | MS.gene66847:intron | 30.0% |
| !! | AGTTTTAGTAGTAGACTAGA+AGG | - | chr3.3:95479515-95479534 | None:intergenic | 30.0% |
| !! | TTTTCCTGATGATGAAATTG+TGG | + | chr3.3:95479979-95479998 | MS.gene66847:intron | 30.0% |
| AAACCCACAATTTCATCATC+AGG | - | chr3.3:95479986-95480005 | None:intergenic | 35.0% | |
| AAATATGTTCGCAGTGAGTT+GGG | + | chr3.3:95479297-95479316 | MS.gene66847:CDS | 35.0% | |
| AATGACCTGGAAGTTTGAAT+GGG | - | chr3.3:95480315-95480334 | None:intergenic | 35.0% | |
| ACTTGAGTGAAAATTTGTGC+TGG | - | chr3.3:95479574-95479593 | None:intergenic | 35.0% | |
| AGAACCACCGTAAAAAGAAA+CGG | - | chr3.3:95479415-95479434 | None:intergenic | 35.0% | |
| AGAACCATCTCATAATTGTG+GGG | - | chr3.3:95479779-95479798 | None:intergenic | 35.0% | |
| ATTAACCCACATAGCAACAA+GGG | - | chr3.3:95479809-95479828 | None:intergenic | 35.0% | |
| CAAGAAATTGTCCATACAAG+AGG | - | chr3.3:95479892-95479911 | None:intergenic | 35.0% | |
| CTAGCAATGCGTTATATCAA+TGG | + | chr3.3:95479056-95479075 | MS.gene66847:intron | 35.0% | |
| CTTGAGTGAAAATTTGTGCT+GGG | - | chr3.3:95479573-95479592 | None:intergenic | 35.0% | |
| GAGTGAAACAAGCAAAAACA+TGG | + | chr3.3:95480364-95480383 | MS.gene66847:CDS | 35.0% | |
| GTTGTGAGCTGTTATTGATA+TGG | + | chr3.3:95480281-95480300 | MS.gene66847:intron | 35.0% | |
| TCTCTTTGAATACGAACTGA+GGG | - | chr3.3:95479623-95479642 | None:intergenic | 35.0% | |
| TGGCAGTTCCAACATATATA+TGG | - | chr3.3:95479720-95479739 | None:intergenic | 35.0% | |
| TTAGTTCATCACTACGCATA+GGG | + | chr3.3:95479679-95479698 | MS.gene66847:intron | 35.0% | |
| TTCTCTTTGAATACGAACTG+AGG | - | chr3.3:95479624-95479643 | None:intergenic | 35.0% | |
| TTTAGTAGTAGACTAGAAGG+CGG | - | chr3.3:95479512-95479531 | None:intergenic | 35.0% | |
| ! | AAGTTCTCTCCGAATTTGTT+TGG | - | chr3.3:95478808-95478827 | None:intergenic | 35.0% |
| ! | ACTTTTAGTGTAGGTCGTTA+GGG | + | chr3.3:95479206-95479225 | MS.gene66847:intron | 35.0% |
| ! | AGGGTGTATGTATCTGTTTT+TGG | + | chr3.3:95479225-95479244 | MS.gene66847:CDS | 35.0% |
| ! | TTTACCGTTTCTTTTTACGG+TGG | + | chr3.3:95479408-95479427 | MS.gene66847:intron | 35.0% |
| !!! | CTGTTTTTGGAGATGAAAGA+GGG | + | chr3.3:95479238-95479257 | MS.gene66847:CDS | 35.0% |
| !!! | TCTGTTTTTGGAGATGAAAG+AGG | + | chr3.3:95479237-95479256 | MS.gene66847:CDS | 35.0% |
| AACTAAGAGATGGTCTGGAA+AGG | - | chr3.3:95479483-95479502 | None:intergenic | 40.0% | |
| AAGCAAAAACATGGAGCAGT+TGG | + | chr3.3:95480373-95480392 | MS.gene66847:CDS | 40.0% | |
| AATATGTTCGCAGTGAGTTG+GGG | + | chr3.3:95479298-95479317 | MS.gene66847:CDS | 40.0% | |
| ACGGAATAAACTGTGACACA+TGG | - | chr3.3:95479740-95479759 | None:intergenic | 40.0% | |
| ACTAAGAGATGGTCTGGAAA+GGG | - | chr3.3:95479482-95479501 | None:intergenic | 40.0% | |
| ATGACTGTCATGTAAAGCTG+TGG | + | chr3.3:95479163-95479182 | MS.gene66847:intron | 40.0% | |
| CAAATATGTTCGCAGTGAGT+TGG | + | chr3.3:95479296-95479315 | MS.gene66847:CDS | 40.0% | |
| CAACTCACTGCGAACATATT+TGG | - | chr3.3:95479298-95479317 | None:intergenic | 40.0% | |
| CAAGAAACTAAGAGATGGTC+TGG | - | chr3.3:95479488-95479507 | None:intergenic | 40.0% | |
| CAATGACCTGGAAGTTTGAA+TGG | - | chr3.3:95480316-95480335 | None:intergenic | 40.0% | |
| CAGTTGGATTCATCAGTGAA+CGG | + | chr3.3:95480389-95480408 | MS.gene66847:CDS | 40.0% | |
| CATTAACCCACATAGCAACA+AGG | - | chr3.3:95479810-95479829 | None:intergenic | 40.0% | |
| GGCAAATTGCAAGACGTTAT+CGG | - | chr3.3:95478847-95478866 | None:intergenic | 40.0% | |
| GTTAGTTCATCACTACGCAT+AGG | + | chr3.3:95479678-95479697 | MS.gene66847:intron | 40.0% | |
| GTTTCCCCACAATTATGAGA+TGG | + | chr3.3:95479772-95479791 | MS.gene66847:intron | 40.0% | |
| TAAGGAAGCTTCAGGAAGAA+CGG | - | chr3.3:95478868-95478887 | None:intergenic | 40.0% | |
| TCTCATAATTGTGGGGAAAC+CGG | - | chr3.3:95479772-95479791 | None:intergenic | 40.0% | |
| TTTATTCCGTGATTTCCTGC+CGG | + | chr3.3:95479750-95479769 | MS.gene66847:intron | 40.0% | |
| ! | AGATGAAGACGATGCTGATT+GGG | + | chr3.3:95480442-95480461 | MS.gene66847:CDS | 40.0% |
| ! | ATCCTCCAAAAAGCGTATCT+CGG | - | chr3.3:95479349-95479368 | None:intergenic | 40.0% |
| ! | CACTTTTAGTGTAGGTCGTT+AGG | + | chr3.3:95479205-95479224 | MS.gene66847:intron | 40.0% |
| ! | GATTTTCACTGCCTCTTGTA+TGG | + | chr3.3:95479878-95479897 | MS.gene66847:intron | 40.0% |
| ! | GTGTTGTTCTGTTTCCACTT+TGG | + | chr3.3:95479456-95479475 | MS.gene66847:intron | 40.0% |
| !! | AATCTTTTGAGAGAGGAAGC+AGG | + | chr3.3:95479370-95479389 | MS.gene66847:CDS | 40.0% |
| !! | ATCTTTTGAGAGAGGAAGCA+GGG | + | chr3.3:95479371-95479390 | MS.gene66847:CDS | 40.0% |
| !! | CACTCAAGTTGTTTCTGTGT+TGG | + | chr3.3:95479585-95479604 | MS.gene66847:intron | 40.0% |
| !! | GAGGATGAATCTTTTGAGAG+AGG | + | chr3.3:95479363-95479382 | MS.gene66847:CDS | 40.0% |
| !! | TGAAATCACCGCTGATTTAC+AGG | + | chr3.3:95478932-95478951 | MS.gene66847:CDS | 40.0% |
| !! | TTTTGGAGATGAAAGAGGGA+AGG | + | chr3.3:95479242-95479261 | MS.gene66847:CDS | 40.0% |
| !!! | TTTTGATTTCAACCCAGCCA+TGG | - | chr3.3:95479274-95479293 | None:intergenic | 40.0% |
| AGATGAAAGAGGGAAGGAAG+TGG | + | chr3.3:95479248-95479267 | MS.gene66847:CDS | 45.0% | |
| ATCAGCGGTGATTTCAACGT+CGG | - | chr3.3:95478928-95478947 | None:intergenic | 45.0% | |
| CAGATGAAGACGATGCTGAT+TGG | + | chr3.3:95480441-95480460 | MS.gene66847:CDS | 45.0% | |
| CTGTCATGTAAAGCTGTGGT+TGG | + | chr3.3:95479167-95479186 | MS.gene66847:intron | 45.0% | |
| GAGGTAAGAATCAGCACCTA+AGG | - | chr3.3:95478886-95478905 | None:intergenic | 45.0% | |
| GATTTCAACGTCGGTGATAG+TGG | - | chr3.3:95478919-95478938 | None:intergenic | 45.0% | |
| GCGGTCAAGAAACTAAGAGA+TGG | - | chr3.3:95479493-95479512 | None:intergenic | 45.0% | |
| GGCGGCGAAAATGATAGAAT+AGG | - | chr3.3:95478972-95478991 | None:intergenic | 45.0% | |
| GTTCTTCCTGAAGCTTCCTT+AGG | + | chr3.3:95478867-95478886 | MS.gene66847:CDS | 45.0% | |
| TTCCACCCATTCAAACTTCC+AGG | + | chr3.3:95480307-95480326 | MS.gene66847:intron | 45.0% | |
| TTGTGGGTTTCACATCACTG+TGG | + | chr3.3:95479996-95480015 | MS.gene66847:intron | 45.0% | |
| ! | AACTCCCGAGATACGCTTTT+TGG | + | chr3.3:95479341-95479360 | MS.gene66847:CDS | 45.0% |
| ! | TCCTCCAAAAAGCGTATCTC+GGG | - | chr3.3:95479348-95479367 | None:intergenic | 45.0% |
| !! | AACAATGCTTTTGAGGGGAG+AGG | + | chr3.3:95479921-95479940 | MS.gene66847:intron | 45.0% |
| !! | ACAATGCTTTTGAGGGGAGA+GGG | + | chr3.3:95479922-95479941 | MS.gene66847:intron | 45.0% |
| ACTAGGCCCTTGTTGCTATG+TGG | + | chr3.3:95479800-95479819 | MS.gene66847:intron | 50.0% | |
| ATAATTGTGGGGAAACCGGC+AGG | - | chr3.3:95479768-95479787 | None:intergenic | 50.0% | |
| CTAGGCCCTTGTTGCTATGT+GGG | + | chr3.3:95479801-95479820 | MS.gene66847:intron | 50.0% | |
| GACCTGGAAGTTTGAATGGG+TGG | - | chr3.3:95480312-95480331 | None:intergenic | 50.0% | |
| TCAGCACCTAAGGAAGCTTC+AGG | - | chr3.3:95478876-95478895 | None:intergenic | 50.0% | |
| !! | AACGTCGGTGATAGTGGTGA+CGG | - | chr3.3:95478913-95478932 | None:intergenic | 50.0% |
| !! | GCATAAGTGAGCACTGGCTA+TGG | - | chr3.3:95480202-95480221 | None:intergenic | 50.0% |
| !! | TCCCGAGATACGCTTTTTGG+AGG | + | chr3.3:95479344-95479363 | MS.gene66847:CDS | 50.0% |
| !! | TGATAGTGGTGACGGAAGAG+AGG | - | chr3.3:95478905-95478924 | None:intergenic | 50.0% |
| AAGAGGGAAGGAAGTGGCCA+TGG | + | chr3.3:95479254-95479273 | MS.gene66847:CDS | 55.0% | |
| GGGAAACCGGCAGGAAATCA+CGG | - | chr3.3:95479759-95479778 | None:intergenic | 55.0% | |
| TGGCGTGCATAAGTGAGCAC+TGG | - | chr3.3:95480208-95480227 | None:intergenic | 55.0% | |
| TGGTCTGGAAAGGGCCAAAG+TGG | - | chr3.3:95479473-95479492 | None:intergenic | 55.0% | |
| ! | ATGTTCGCAGTGAGTTGGGG+AGG | + | chr3.3:95479301-95479320 | MS.gene66847:CDS | 55.0% |
| ! | TGGGGAGGCGTATGAAGTTG+CGG | + | chr3.3:95479316-95479335 | MS.gene66847:CDS | 55.0% |
| !! | ATGCTTTTGAGGGGAGAGGG+TGG | + | chr3.3:95479925-95479944 | MS.gene66847:intron | 55.0% |
| !! | TGCTTTTGAGGGGAGAGGGT+GGG | + | chr3.3:95479926-95479945 | MS.gene66847:intron | 55.0% |
| GGAAGGAAGTGGCCATGGCT+GGG | + | chr3.3:95479259-95479278 | MS.gene66847:CDS | 60.0% | |
| ! | AGGGGAGAGGGTGGGTTCTT+TGG | + | chr3.3:95479934-95479953 | MS.gene66847:intron | 60.0% |
| GGGAAGGAAGTGGCCATGGC+TGG | + | chr3.3:95479258-95479277 | MS.gene66847:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 95478798 | 95480485 | 95478798 | ID=MS.gene66847 |
| chr3.3 | mRNA | 95478798 | 95480485 | 95478798 | ID=MS.gene66847.t1;Parent=MS.gene66847 |
| chr3.3 | exon | 95478798 | 95478953 | 95478798 | ID=MS.gene66847.t1.exon1;Parent=MS.gene66847.t1 |
| chr3.3 | CDS | 95478798 | 95478953 | 95478798 | ID=cds.MS.gene66847.t1;Parent=MS.gene66847.t1 |
| chr3.3 | exon | 95479219 | 95479392 | 95479219 | ID=MS.gene66847.t1.exon2;Parent=MS.gene66847.t1 |
| chr3.3 | CDS | 95479219 | 95479392 | 95479219 | ID=cds.MS.gene66847.t1;Parent=MS.gene66847.t1 |
| chr3.3 | exon | 95480329 | 95480485 | 95480329 | ID=MS.gene66847.t1.exon3;Parent=MS.gene66847.t1 |
| chr3.3 | CDS | 95480329 | 95480485 | 95480329 | ID=cds.MS.gene66847.t1;Parent=MS.gene66847.t1 |
| Gene Sequence |
| Protein sequence |
