Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063114.t1 | XP_003604015.1 | 98.2 | 168 | 3 | 0 | 1 | 168 | 62 | 229 | 2.10E-68 | 268.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063114.t1 | O65693 | 66.7 | 168 | 42 | 1 | 1 | 168 | 62 | 215 | 2.3e-50 | 199.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063114.t1 | G7J7U0 | 98.2 | 168 | 3 | 0 | 1 | 168 | 62 | 229 | 1.5e-68 | 268.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049134 | MS.gene063114 | -0.802348 | 5.85E-49 | -1.69E-46 |
| MS.gene049149 | MS.gene063114 | 0.820783 | 5.71E-53 | -1.69E-46 |
| MS.gene049150 | MS.gene063114 | 0.830072 | 3.61E-55 | -1.69E-46 |
| MS.gene049153 | MS.gene063114 | 0.823744 | 1.17E-53 | -1.69E-46 |
| MS.gene049284 | MS.gene063114 | 0.815125 | 1.08E-51 | -1.69E-46 |
| MS.gene049297 | MS.gene063114 | 0.815945 | 7.13E-52 | -1.69E-46 |
| MS.gene049346 | MS.gene063114 | 0.839733 | 1.33E-57 | -1.69E-46 |
| MS.gene049351 | MS.gene063114 | 0.821964 | 3.05E-53 | -1.69E-46 |
| MS.gene049426 | MS.gene063114 | 0.806849 | 6.74E-50 | -1.69E-46 |
| MS.gene049491 | MS.gene063114 | 0.828232 | 1.01E-54 | -1.69E-46 |
| MS.gene049661 | MS.gene063114 | 0.841497 | 4.58E-58 | -1.69E-46 |
| MS.gene049662 | MS.gene063114 | 0.83858 | 2.64E-57 | -1.69E-46 |
| MS.gene049758 | MS.gene063114 | 0.844585 | 6.90E-59 | -1.69E-46 |
| MS.gene049910 | MS.gene063114 | 0.876831 | 1.02E-68 | -1.69E-46 |
| MS.gene049915 | MS.gene063114 | 0.809746 | 1.62E-50 | -1.69E-46 |
| MS.gene049993 | MS.gene063114 | 0.823783 | 1.15E-53 | -1.69E-46 |
| MS.gene050076 | MS.gene063114 | 0.801104 | 1.05E-48 | -1.69E-46 |
| MS.gene050227 | MS.gene063114 | 0.800754 | 1.24E-48 | -1.69E-46 |
| MS.gene050231 | MS.gene063114 | 0.824471 | 7.93E-54 | -1.69E-46 |
| MS.gene050301 | MS.gene063114 | 0.893804 | 4.47E-75 | -1.69E-46 |
| MS.gene050330 | MS.gene063114 | -0.804984 | 1.66E-49 | -1.69E-46 |
| MS.gene050397 | MS.gene063114 | 0.850342 | 1.81E-60 | -1.69E-46 |
| MS.gene050404 | MS.gene063114 | 0.80451 | 2.09E-49 | -1.69E-46 |
| MS.gene050406 | MS.gene063114 | 0.844079 | 9.44E-59 | -1.69E-46 |
| MS.gene050426 | MS.gene063114 | -0.827961 | 1.17E-54 | -1.69E-46 |
| MS.gene050464 | MS.gene063114 | 0.876291 | 1.57E-68 | -1.69E-46 |
| MS.gene050529 | MS.gene063114 | 0.864906 | 8.68E-65 | -1.69E-46 |
| MS.gene050558 | MS.gene063114 | 0.860734 | 1.68E-63 | -1.69E-46 |
| MS.gene050560 | MS.gene063114 | 0.822722 | 2.03E-53 | -1.69E-46 |
| MS.gene050625 | MS.gene063114 | 0.808492 | 3.02E-50 | -1.69E-46 |
| MS.gene050642 | MS.gene063114 | 0.824169 | 9.34E-54 | -1.69E-46 |
| MS.gene050650 | MS.gene063114 | 0.821131 | 4.75E-53 | -1.69E-46 |
| MS.gene050670 | MS.gene063114 | 0.863756 | 1.98E-64 | -1.69E-46 |
| MS.gene050787 | MS.gene063114 | 0.814398 | 1.57E-51 | -1.69E-46 |
| MS.gene050820 | MS.gene063114 | 0.82805 | 1.11E-54 | -1.69E-46 |
| MS.gene05101 | MS.gene063114 | 0.861145 | 1.26E-63 | -1.69E-46 |
| MS.gene05105 | MS.gene063114 | 0.832061 | 1.17E-55 | -1.69E-46 |
| MS.gene051471 | MS.gene063114 | 0.829313 | 5.52E-55 | -1.69E-46 |
| MS.gene051525 | MS.gene063114 | 0.854064 | 1.58E-61 | -1.69E-46 |
| MS.gene051582 | MS.gene063114 | 0.848514 | 5.84E-60 | -1.69E-46 |
| MS.gene051663 | MS.gene063114 | 0.803953 | 2.73E-49 | -1.69E-46 |
| MS.gene051860 | MS.gene063114 | 0.88415 | 2.45E-71 | -1.69E-46 |
| MS.gene051983 | MS.gene063114 | 0.833725 | 4.52E-56 | -1.69E-46 |
| MS.gene052011 | MS.gene063114 | -0.864513 | 1.15E-64 | -1.69E-46 |
| MS.gene052077 | MS.gene063114 | 0.861887 | 7.48E-64 | -1.69E-46 |
| MS.gene052078 | MS.gene063114 | 0.833135 | 6.34E-56 | -1.69E-46 |
| MS.gene052178 | MS.gene063114 | 0.840904 | 6.57E-58 | -1.69E-46 |
| MS.gene052188 | MS.gene063114 | 0.868888 | 4.67E-66 | -1.69E-46 |
| MS.gene052207 | MS.gene063114 | 0.815889 | 7.34E-52 | -1.69E-46 |
| MS.gene052253 | MS.gene063114 | 0.901265 | 3.19E-78 | -1.69E-46 |
| MS.gene052255 | MS.gene063114 | 0.81961 | 1.06E-52 | -1.69E-46 |
| MS.gene052310 | MS.gene063114 | 0.86784 | 1.02E-65 | -1.69E-46 |
| MS.gene052322 | MS.gene063114 | 0.818644 | 1.76E-52 | -1.69E-46 |
| MS.gene052340 | MS.gene063114 | 0.876199 | 1.69E-68 | -1.69E-46 |
| MS.gene052481 | MS.gene063114 | 0.86171 | 8.47E-64 | -1.69E-46 |
| MS.gene052485 | MS.gene063114 | 0.846764 | 1.77E-59 | -1.69E-46 |
| MS.gene052917 | MS.gene063114 | -0.801618 | 8.27E-49 | -1.69E-46 |
| MS.gene053103 | MS.gene063114 | 0.802503 | 5.44E-49 | -1.69E-46 |
| MS.gene053209 | MS.gene063114 | 0.820971 | 5.17E-53 | -1.69E-46 |
| MS.gene053557 | MS.gene063114 | 0.854724 | 1.02E-61 | -1.69E-46 |
| MS.gene053836 | MS.gene063114 | 0.903178 | 4.53E-79 | -1.69E-46 |
| MS.gene053971 | MS.gene063114 | 0.807802 | 4.23E-50 | -1.69E-46 |
| MS.gene054010 | MS.gene063114 | 0.827885 | 1.22E-54 | -1.69E-46 |
| MS.gene054011 | MS.gene063114 | 0.850158 | 2.03E-60 | -1.69E-46 |
| MS.gene054012 | MS.gene063114 | 0.835589 | 1.54E-56 | -1.69E-46 |
| MS.gene054013 | MS.gene063114 | 0.828486 | 8.75E-55 | -1.69E-46 |
| MS.gene054123 | MS.gene063114 | 0.815153 | 1.07E-51 | -1.69E-46 |
| MS.gene054183 | MS.gene063114 | 0.872721 | 2.56E-67 | -1.69E-46 |
| MS.gene054185 | MS.gene063114 | 0.800644 | 1.31E-48 | -1.69E-46 |
| MS.gene054349 | MS.gene063114 | 0.824146 | 9.45E-54 | -1.69E-46 |
| MS.gene054473 | MS.gene063114 | 0.86482 | 9.23E-65 | -1.69E-46 |
| MS.gene054542 | MS.gene063114 | 0.803005 | 4.29E-49 | -1.69E-46 |
| MS.gene05455 | MS.gene063114 | 0.813797 | 2.13E-51 | -1.69E-46 |
| MS.gene054719 | MS.gene063114 | 0.822822 | 1.93E-53 | -1.69E-46 |
| MS.gene054917 | MS.gene063114 | 0.815195 | 1.05E-51 | -1.69E-46 |
| MS.gene054918 | MS.gene063114 | 0.824258 | 8.90E-54 | -1.69E-46 |
| MS.gene054930 | MS.gene063114 | 0.820002 | 8.63E-53 | -1.69E-46 |
| MS.gene054955 | MS.gene063114 | 0.80089 | 1.17E-48 | -1.69E-46 |
| MS.gene054983 | MS.gene063114 | 0.85723 | 1.88E-62 | -1.69E-46 |
| MS.gene055116 | MS.gene063114 | 0.889219 | 2.95E-73 | -1.69E-46 |
| MS.gene055277 | MS.gene063114 | 0.858928 | 5.88E-63 | -1.69E-46 |
| MS.gene055390 | MS.gene063114 | 0.815907 | 7.27E-52 | -1.69E-46 |
| MS.gene055422 | MS.gene063114 | 0.876176 | 1.72E-68 | -1.69E-46 |
| MS.gene055428 | MS.gene063114 | 0.870653 | 1.24E-66 | -1.69E-46 |
| MS.gene05553 | MS.gene063114 | 0.837886 | 3.99E-57 | -1.69E-46 |
| MS.gene055601 | MS.gene063114 | -0.813961 | 1.96E-51 | -1.69E-46 |
| MS.gene055604 | MS.gene063114 | 0.837421 | 5.25E-57 | -1.69E-46 |
| MS.gene056191 | MS.gene063114 | 0.865932 | 4.13E-65 | -1.69E-46 |
| MS.gene056407 | MS.gene063114 | 0.834732 | 2.53E-56 | -1.69E-46 |
| MS.gene056451 | MS.gene063114 | 0.824989 | 5.98E-54 | -1.69E-46 |
| MS.gene056497 | MS.gene063114 | 0.803298 | 3.73E-49 | -1.69E-46 |
| MS.gene056511 | MS.gene063114 | 0.801706 | 7.93E-49 | -1.69E-46 |
| MS.gene056612 | MS.gene063114 | 0.85257 | 4.23E-61 | -1.69E-46 |
| MS.gene056659 | MS.gene063114 | 0.822054 | 2.91E-53 | -1.69E-46 |
| MS.gene056723 | MS.gene063114 | 0.841827 | 3.75E-58 | -1.69E-46 |
| MS.gene056846 | MS.gene063114 | 0.801991 | 6.93E-49 | -1.69E-46 |
| MS.gene056899 | MS.gene063114 | -0.860261 | 2.34E-63 | -1.69E-46 |
| MS.gene056979 | MS.gene063114 | 0.833838 | 4.24E-56 | -1.69E-46 |
| MS.gene05702 | MS.gene063114 | 0.872697 | 2.61E-67 | -1.69E-46 |
| MS.gene057121 | MS.gene063114 | 0.866994 | 1.90E-65 | -1.69E-46 |
| MS.gene057123 | MS.gene063114 | 0.813013 | 3.17E-51 | -1.69E-46 |
| MS.gene057124 | MS.gene063114 | 0.802146 | 6.44E-49 | -1.69E-46 |
| MS.gene057146 | MS.gene063114 | 0.809403 | 1.93E-50 | -1.69E-46 |
| MS.gene057245 | MS.gene063114 | 0.860795 | 1.61E-63 | -1.69E-46 |
| MS.gene057310 | MS.gene063114 | 0.801276 | 9.72E-49 | -1.69E-46 |
| MS.gene05733 | MS.gene063114 | 0.825838 | 3.77E-54 | -1.69E-46 |
| MS.gene057396 | MS.gene063114 | 0.815778 | 7.76E-52 | -1.69E-46 |
| MS.gene057439 | MS.gene063114 | 0.814555 | 1.45E-51 | -1.69E-46 |
| MS.gene057448 | MS.gene063114 | 0.829252 | 5.71E-55 | -1.69E-46 |
| MS.gene057449 | MS.gene063114 | 0.821408 | 4.10E-53 | -1.69E-46 |
| MS.gene057450 | MS.gene063114 | 0.82217 | 2.73E-53 | -1.69E-46 |
| MS.gene057469 | MS.gene063114 | 0.821471 | 3.97E-53 | -1.69E-46 |
| MS.gene057476 | MS.gene063114 | 0.829175 | 5.96E-55 | -1.69E-46 |
| MS.gene057749 | MS.gene063114 | 0.851108 | 1.10E-60 | -1.69E-46 |
| MS.gene057779 | MS.gene063114 | 0.819042 | 1.43E-52 | -1.69E-46 |
| MS.gene057782 | MS.gene063114 | 0.812463 | 4.19E-51 | -1.69E-46 |
| MS.gene057823 | MS.gene063114 | 0.836981 | 6.80E-57 | -1.69E-46 |
| MS.gene057825 | MS.gene063114 | 0.816436 | 5.54E-52 | -1.69E-46 |
| MS.gene058002 | MS.gene063114 | 0.899174 | 2.57E-77 | -1.69E-46 |
| MS.gene058007 | MS.gene063114 | 0.803049 | 4.20E-49 | -1.69E-46 |
| MS.gene05811 | MS.gene063114 | 0.882334 | 1.14E-70 | -1.69E-46 |
| MS.gene058142 | MS.gene063114 | 0.867466 | 1.34E-65 | -1.69E-46 |
| MS.gene058218 | MS.gene063114 | -0.815825 | 7.58E-52 | -1.69E-46 |
| MS.gene058233 | MS.gene063114 | 0.850916 | 1.25E-60 | -1.69E-46 |
| MS.gene058276 | MS.gene063114 | 0.839675 | 1.38E-57 | -1.69E-46 |
| MS.gene058293 | MS.gene063114 | 0.8082 | 3.48E-50 | -1.69E-46 |
| MS.gene058376 | MS.gene063114 | 0.803117 | 4.06E-49 | -1.69E-46 |
| MS.gene05842 | MS.gene063114 | 0.805746 | 1.15E-49 | -1.69E-46 |
| MS.gene058444 | MS.gene063114 | 0.811908 | 5.53E-51 | -1.69E-46 |
| MS.gene05856 | MS.gene063114 | 0.802493 | 5.46E-49 | -1.69E-46 |
| MS.gene058660 | MS.gene063114 | 0.873469 | 1.44E-67 | -1.69E-46 |
| MS.gene058710 | MS.gene063114 | 0.833062 | 6.62E-56 | -1.69E-46 |
| MS.gene058749 | MS.gene063114 | 0.813297 | 2.75E-51 | -1.69E-46 |
| MS.gene058845 | MS.gene063114 | 0.816176 | 6.33E-52 | -1.69E-46 |
| MS.gene05886 | MS.gene063114 | 0.863358 | 2.64E-64 | -1.69E-46 |
| MS.gene058928 | MS.gene063114 | 0.866853 | 2.10E-65 | -1.69E-46 |
| MS.gene059011 | MS.gene063114 | 0.807817 | 4.20E-50 | -1.69E-46 |
| MS.gene05907 | MS.gene063114 | 0.884559 | 1.73E-71 | -1.69E-46 |
| MS.gene059082 | MS.gene063114 | 0.805043 | 1.61E-49 | -1.69E-46 |
| MS.gene059141 | MS.gene063114 | 0.856517 | 3.05E-62 | -1.69E-46 |
| MS.gene059247 | MS.gene063114 | 0.814343 | 1.62E-51 | -1.69E-46 |
| MS.gene059285 | MS.gene063114 | 0.860572 | 1.88E-63 | -1.69E-46 |
| MS.gene059294 | MS.gene063114 | 0.845038 | 5.21E-59 | -1.69E-46 |
| MS.gene059366 | MS.gene063114 | 0.800624 | 1.32E-48 | -1.69E-46 |
| MS.gene059420 | MS.gene063114 | 0.867822 | 1.03E-65 | -1.69E-46 |
| MS.gene059462 | MS.gene063114 | 0.827388 | 1.61E-54 | -1.69E-46 |
| MS.gene059518 | MS.gene063114 | 0.847831 | 9.02E-60 | -1.69E-46 |
| MS.gene059536 | MS.gene063114 | 0.813451 | 2.54E-51 | -1.69E-46 |
| MS.gene059597 | MS.gene063114 | 0.872797 | 2.41E-67 | -1.69E-46 |
| MS.gene059676 | MS.gene063114 | 0.823723 | 1.19E-53 | -1.69E-46 |
| MS.gene059688 | MS.gene063114 | 0.885637 | 6.85E-72 | -1.69E-46 |
| MS.gene059692 | MS.gene063114 | 0.879326 | 1.37E-69 | -1.69E-46 |
| MS.gene059695 | MS.gene063114 | 0.880425 | 5.55E-70 | -1.69E-46 |
| MS.gene05984 | MS.gene063114 | 0.899923 | 1.22E-77 | -1.69E-46 |
| MS.gene059876 | MS.gene063114 | 0.804821 | 1.80E-49 | -1.69E-46 |
| MS.gene059894 | MS.gene063114 | 0.8508 | 1.34E-60 | -1.69E-46 |
| MS.gene060247 | MS.gene063114 | 0.825334 | 4.96E-54 | -1.69E-46 |
| MS.gene06037 | MS.gene063114 | 0.82628 | 2.96E-54 | -1.69E-46 |
| MS.gene060403 | MS.gene063114 | 0.853789 | 1.89E-61 | -1.69E-46 |
| MS.gene060407 | MS.gene063114 | 0.811352 | 7.31E-51 | -1.69E-46 |
| MS.gene060476 | MS.gene063114 | 0.80299 | 4.32E-49 | -1.69E-46 |
| MS.gene060986 | MS.gene063114 | 0.82437 | 8.38E-54 | -1.69E-46 |
| MS.gene060994 | MS.gene063114 | 0.807502 | 4.90E-50 | -1.69E-46 |
| MS.gene061000 | MS.gene063114 | 0.802055 | 6.73E-49 | -1.69E-46 |
| MS.gene061049 | MS.gene063114 | 0.895408 | 9.86E-76 | -1.69E-46 |
| MS.gene061051 | MS.gene063114 | -0.800002 | 1.77E-48 | -1.69E-46 |
| MS.gene061114 | MS.gene063114 | -0.815433 | 9.27E-52 | -1.69E-46 |
| MS.gene061154 | MS.gene063114 | 0.815302 | 9.91E-52 | -1.69E-46 |
| MS.gene061159 | MS.gene063114 | 0.834295 | 3.26E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063114.t1 | MTR_3g117600 | 98.214 | 168 | 3 | 0 | 1 | 168 | 62 | 229 | 1.87e-117 | 332 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063114.t1 | AT4G34730 | 69.643 | 168 | 37 | 1 | 1 | 168 | 62 | 215 | 5.96e-77 | 228 |
| MS.gene063114.t1 | AT4G34730 | 69.643 | 168 | 37 | 1 | 1 | 168 | 62 | 215 | 5.96e-77 | 228 |
| MS.gene063114.t1 | AT4G34730 | 69.643 | 168 | 37 | 1 | 1 | 168 | 62 | 215 | 5.96e-77 | 228 |
Find 30 sgRNAs with CRISPR-Local
Find 120 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACTCACTGCGAACATATT+TGG | 0.174334 | 3.3:-95489864 | None:intergenic |
| TGAAATCACCGCTGATTTAC+AGG | 0.294810 | 3.3:+95489501 | MS.gene063114:CDS |
| AAATATGTTCGCAGTGAGTT+GGG | 0.366464 | 3.3:+95489866 | MS.gene063114:CDS |
| GGCAAATTGCAAGACGTTAT+CGG | 0.368809 | 3.3:-95489413 | None:intergenic |
| GGGAAGGAAGTGGCCATGGC+TGG | 0.375787 | 3.3:+95489827 | MS.gene063114:CDS |
| GTTCTTCCTGAAGCTTCCTT+AGG | 0.384742 | 3.3:+95489436 | MS.gene063114:CDS |
| AAGTTCTCTCCGAATTTGTT+TGG | 0.408703 | 3.3:-95489374 | None:intergenic |
| TCAGCACCTAAGGAAGCTTC+AGG | 0.415676 | 3.3:-95489442 | None:intergenic |
| ACATAAATGATACCTTCTTC+AGG | 0.417511 | 3.3:-95491045 | None:intergenic |
| AAATGGTTGCCAAACAAATT+CGG | 0.421978 | 3.3:+95489365 | None:intergenic |
| CAGATGAAGACGATGCTGAT+TGG | 0.423415 | 3.3:+95491010 | MS.gene063114:CDS |
| GATTTCAACGTCGGTGATAG+TGG | 0.425429 | 3.3:-95489485 | None:intergenic |
| AGATGAAGACGATGCTGATT+GGG | 0.431019 | 3.3:+95491011 | MS.gene063114:CDS |
| GAGGTAAGAATCAGCACCTA+AGG | 0.457882 | 3.3:-95489452 | None:intergenic |
| CAAATATGTTCGCAGTGAGT+TGG | 0.480055 | 3.3:+95489865 | MS.gene063114:CDS |
| CATAAATGATACCTTCTTCA+GGG | 0.510113 | 3.3:-95491044 | None:intergenic |
| TGAAGAAGGTATCATTTATG+TGG | 0.517227 | 3.3:+95491047 | MS.gene063114:CDS |
| GGAAGGAAGTGGCCATGGCT+GGG | 0.525786 | 3.3:+95489828 | MS.gene063114:CDS |
| AGATGAAAGAGGGAAGGAAG+TGG | 0.566160 | 3.3:+95489817 | MS.gene063114:CDS |
| GAAGATGAAGACCCTGAAGA+AGG | 0.579729 | 3.3:+95491033 | MS.gene063114:CDS |
| AAGAGGGAAGGAAGTGGCCA+TGG | 0.582663 | 3.3:+95489823 | MS.gene063114:CDS |
| TGGGGAGGCGTATGAAGTTG+CGG | 0.591668 | 3.3:+95489885 | MS.gene063114:CDS |
| AATATGTTCGCAGTGAGTTG+GGG | 0.594957 | 3.3:+95489867 | MS.gene063114:CDS |
| TAAGGAAGCTTCAGGAAGAA+CGG | 0.601825 | 3.3:-95489434 | None:intergenic |
| ATGTTCGCAGTGAGTTGGGG+AGG | 0.601873 | 3.3:+95489870 | MS.gene063114:CDS |
| AACGTCGGTGATAGTGGTGA+CGG | 0.620179 | 3.3:-95489479 | None:intergenic |
| TGATAGTGGTGACGGAAGAG+AGG | 0.626472 | 3.3:-95489471 | None:intergenic |
| CAGTTGGATTCATCAGTGAA+CGG | 0.643319 | 3.3:+95490958 | MS.gene063114:CDS |
| ATCAGCGGTGATTTCAACGT+CGG | 0.653697 | 3.3:-95489494 | None:intergenic |
| AATAAGAACCTGTAAATCAG+CGG | 0.693763 | 3.3:-95489509 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACATATAGAAAAAAATATA+AGG | - | chr3.3:95490230-95490249 | None:intergenic | 10.0% |
| !! | ATCATGAAACAAAATAAATA+AGG | - | chr3.3:95489714-95489733 | None:intergenic | 15.0% |
| !! | ATTAAATGAGAAAAATGAAA+AGG | - | chr3.3:95489670-95489689 | None:intergenic | 15.0% |
| !! | TGCTTAAATATATAAAAGAA+TGG | - | chr3.3:95490797-95490816 | None:intergenic | 15.0% |
| !!! | CATATTCGTTAATTTTAAAA+AGG | + | chr3.3:95490660-95490679 | MS.gene063114:intron | 15.0% |
| !! | AAGATAAAAAAAGATTTCAG+CGG | - | chr3.3:95490633-95490652 | None:intergenic | 20.0% |
| !! | GAATTATCACACTATAAATT+CGG | - | chr3.3:95489562-95489581 | None:intergenic | 20.0% |
| !! | TCATCAGGAAAATAAAAATA+AGG | - | chr3.3:95490540-95490559 | None:intergenic | 20.0% |
| !!! | ATATTTACCGTTTCTTTTTA+CGG | + | chr3.3:95489974-95489993 | MS.gene063114:intron | 20.0% |
| ! | AGTGATACATAAGAATAAGA+TGG | + | chr3.3:95490418-95490437 | MS.gene063114:intron | 25.0% |
| ! | CTCATTTAATCTAGTGTATT+TGG | + | chr3.3:95489680-95489699 | MS.gene063114:intron | 25.0% |
| ! | TATATATTTAAGCAGCTTCT+TGG | + | chr3.3:95490803-95490822 | MS.gene063114:intron | 25.0% |
| ! | TCTATCTACCATATATATGT+TGG | + | chr3.3:95490278-95490297 | MS.gene063114:intron | 25.0% |
| ! | TTTATAGTGTGATAATTCAG+TGG | + | chr3.3:95489564-95489583 | MS.gene063114:intron | 25.0% |
| !! | TATTCAAACAATGCTTTTGA+GGG | + | chr3.3:95490484-95490503 | MS.gene063114:intron | 25.0% |
| AAGAACCATCTCATAATTGT+GGG | - | chr3.3:95490349-95490368 | None:intergenic | 30.0% | |
| AATAAGAACCTGTAAATCAG+CGG | - | chr3.3:95489512-95489531 | None:intergenic | 30.0% | |
| ACATAAATGATACCTTCTTC+AGG | - | chr3.3:95491048-95491067 | None:intergenic | 30.0% | |
| AGAAAAATGAAAAGGCAATG+TGG | - | chr3.3:95489662-95489681 | None:intergenic | 30.0% | |
| ATTATGAGATGGTTCTTACT+AGG | + | chr3.3:95490352-95490371 | MS.gene063114:intron | 30.0% | |
| CATAAATGATACCTTCTTCA+GGG | - | chr3.3:95491047-95491066 | None:intergenic | 30.0% | |
| TAAGAACCATCTCATAATTG+TGG | - | chr3.3:95490350-95490369 | None:intergenic | 30.0% | |
| TATCTAATATTGCAATGACC+TGG | - | chr3.3:95490897-95490916 | None:intergenic | 30.0% | |
| TTATCACACTATAAATTCGG+CGG | - | chr3.3:95489559-95489578 | None:intergenic | 30.0% | |
| ! | ATTCAAACAATGCTTTTGAG+GGG | + | chr3.3:95490485-95490504 | MS.gene063114:intron | 30.0% |
| ! | CTATTCAAACAATGCTTTTG+AGG | + | chr3.3:95490483-95490502 | MS.gene063114:intron | 30.0% |
| ! | TAACTCAACACTTTTAGTGT+AGG | + | chr3.3:95489766-95489785 | MS.gene063114:intron | 30.0% |
| ! | TTTCCTGATGATGAAATTGT+GGG | + | chr3.3:95490549-95490568 | MS.gene063114:intron | 30.0% |
| !! | AGTTTTAGTAGTAGACTAGA+AGG | - | chr3.3:95490084-95490103 | None:intergenic | 30.0% |
| !! | TGAAGAAGGTATCATTTATG+TGG | + | chr3.3:95491047-95491066 | MS.gene063114:CDS | 30.0% |
| !! | TTTTCCTGATGATGAAATTG+TGG | + | chr3.3:95490548-95490567 | MS.gene063114:intron | 30.0% |
| AAACCCACAATTTCATCATC+AGG | - | chr3.3:95490555-95490574 | None:intergenic | 35.0% | |
| AAATATGTTCGCAGTGAGTT+GGG | + | chr3.3:95489866-95489885 | MS.gene063114:CDS | 35.0% | |
| AATGACCTGGAAGTTTGAAT+GGG | - | chr3.3:95490884-95490903 | None:intergenic | 35.0% | |
| ACTTGAGTGAAAATTTGTGC+TGG | - | chr3.3:95490143-95490162 | None:intergenic | 35.0% | |
| AGAACCACCGTAAAAAGAAA+CGG | - | chr3.3:95489984-95490003 | None:intergenic | 35.0% | |
| AGAACCATCTCATAATTGTG+GGG | - | chr3.3:95490348-95490367 | None:intergenic | 35.0% | |
| ATTAACCCACATAGCAACAA+GGG | - | chr3.3:95490378-95490397 | None:intergenic | 35.0% | |
| CAAGAAATTGTCCATACAAG+AGG | - | chr3.3:95490461-95490480 | None:intergenic | 35.0% | |
| CTAGCAATGCGTTATATCAA+TGG | + | chr3.3:95489625-95489644 | MS.gene063114:intron | 35.0% | |
| CTTGAGTGAAAATTTGTGCT+GGG | - | chr3.3:95490142-95490161 | None:intergenic | 35.0% | |
| GAGTGAAACAAGCAAAAACA+TGG | + | chr3.3:95490933-95490952 | MS.gene063114:CDS | 35.0% | |
| GTTGTGAGCTGTTATTGATA+TGG | + | chr3.3:95490850-95490869 | MS.gene063114:intron | 35.0% | |
| TCTCTTTGAATACGAACTGA+GGG | - | chr3.3:95490192-95490211 | None:intergenic | 35.0% | |
| TGGCAGTTCCAACATATATA+TGG | - | chr3.3:95490289-95490308 | None:intergenic | 35.0% | |
| TTAGTTCATCACTACGCATA+GGG | + | chr3.3:95490248-95490267 | MS.gene063114:intron | 35.0% | |
| TTCTCTTTGAATACGAACTG+AGG | - | chr3.3:95490193-95490212 | None:intergenic | 35.0% | |
| TTTAGTAGTAGACTAGAAGG+CGG | - | chr3.3:95490081-95490100 | None:intergenic | 35.0% | |
| ! | AAGTTCTCTCCGAATTTGTT+TGG | - | chr3.3:95489377-95489396 | None:intergenic | 35.0% |
| ! | ACTTTTAGTGTAGGTCGTTA+GGG | + | chr3.3:95489775-95489794 | MS.gene063114:intron | 35.0% |
| ! | AGGGTGTATGTATCTGTTTT+TGG | + | chr3.3:95489794-95489813 | MS.gene063114:CDS | 35.0% |
| ! | TTTACCGTTTCTTTTTACGG+TGG | + | chr3.3:95489977-95489996 | MS.gene063114:intron | 35.0% |
| !!! | CTGTTTTTGGAGATGAAAGA+GGG | + | chr3.3:95489807-95489826 | MS.gene063114:CDS | 35.0% |
| !!! | TCTGTTTTTGGAGATGAAAG+AGG | + | chr3.3:95489806-95489825 | MS.gene063114:CDS | 35.0% |
| AACTAAGAGATGGTCTGGAA+AGG | - | chr3.3:95490052-95490071 | None:intergenic | 40.0% | |
| AAGCAAAAACATGGAGCAGT+TGG | + | chr3.3:95490942-95490961 | MS.gene063114:CDS | 40.0% | |
| AATATGTTCGCAGTGAGTTG+GGG | + | chr3.3:95489867-95489886 | MS.gene063114:CDS | 40.0% | |
| ACGGAATAAACTGTGACACA+TGG | - | chr3.3:95490309-95490328 | None:intergenic | 40.0% | |
| ACTAAGAGATGGTCTGGAAA+GGG | - | chr3.3:95490051-95490070 | None:intergenic | 40.0% | |
| ATGACTGTCATGTAAAGCTG+TGG | + | chr3.3:95489732-95489751 | MS.gene063114:intron | 40.0% | |
| CAAATATGTTCGCAGTGAGT+TGG | + | chr3.3:95489865-95489884 | MS.gene063114:CDS | 40.0% | |
| CAACTCACTGCGAACATATT+TGG | - | chr3.3:95489867-95489886 | None:intergenic | 40.0% | |
| CAAGAAACTAAGAGATGGTC+TGG | - | chr3.3:95490057-95490076 | None:intergenic | 40.0% | |
| CAATGACCTGGAAGTTTGAA+TGG | - | chr3.3:95490885-95490904 | None:intergenic | 40.0% | |
| CAGTTGGATTCATCAGTGAA+CGG | + | chr3.3:95490958-95490977 | MS.gene063114:CDS | 40.0% | |
| CATTAACCCACATAGCAACA+AGG | - | chr3.3:95490379-95490398 | None:intergenic | 40.0% | |
| GGCAAATTGCAAGACGTTAT+CGG | - | chr3.3:95489416-95489435 | None:intergenic | 40.0% | |
| GTTAGTTCATCACTACGCAT+AGG | + | chr3.3:95490247-95490266 | MS.gene063114:intron | 40.0% | |
| GTTTCCCCACAATTATGAGA+TGG | + | chr3.3:95490341-95490360 | MS.gene063114:intron | 40.0% | |
| TAAGGAAGCTTCAGGAAGAA+CGG | - | chr3.3:95489437-95489456 | None:intergenic | 40.0% | |
| TCTCATAATTGTGGGGAAAC+CGG | - | chr3.3:95490341-95490360 | None:intergenic | 40.0% | |
| TTTATTCCGTGATTTCCTGC+CGG | + | chr3.3:95490319-95490338 | MS.gene063114:intron | 40.0% | |
| ! | AGATGAAGACGATGCTGATT+GGG | + | chr3.3:95491011-95491030 | MS.gene063114:CDS | 40.0% |
| ! | ATCCTCCAAAAAGCGTATCT+CGG | - | chr3.3:95489918-95489937 | None:intergenic | 40.0% |
| ! | CACTTTTAGTGTAGGTCGTT+AGG | + | chr3.3:95489774-95489793 | MS.gene063114:intron | 40.0% |
| ! | GATTTTCACTGCCTCTTGTA+TGG | + | chr3.3:95490447-95490466 | MS.gene063114:intron | 40.0% |
| ! | GTGTTGTTCTGTTTCCACTT+TGG | + | chr3.3:95490025-95490044 | MS.gene063114:intron | 40.0% |
| !! | AATCTTTTGAGAGAGGAAGC+AGG | + | chr3.3:95489939-95489958 | MS.gene063114:CDS | 40.0% |
| !! | ATCTTTTGAGAGAGGAAGCA+GGG | + | chr3.3:95489940-95489959 | MS.gene063114:CDS | 40.0% |
| !! | CACTCAAGTTGTTTCTGTGT+TGG | + | chr3.3:95490154-95490173 | MS.gene063114:intron | 40.0% |
| !! | GAGGATGAATCTTTTGAGAG+AGG | + | chr3.3:95489932-95489951 | MS.gene063114:CDS | 40.0% |
| !! | TGAAATCACCGCTGATTTAC+AGG | + | chr3.3:95489501-95489520 | MS.gene063114:CDS | 40.0% |
| !! | TTTTGGAGATGAAAGAGGGA+AGG | + | chr3.3:95489811-95489830 | MS.gene063114:CDS | 40.0% |
| !!! | TTTTGATTTCAACCCAGCCA+TGG | - | chr3.3:95489843-95489862 | None:intergenic | 40.0% |
| AGATGAAAGAGGGAAGGAAG+TGG | + | chr3.3:95489817-95489836 | MS.gene063114:CDS | 45.0% | |
| ATCAGCGGTGATTTCAACGT+CGG | - | chr3.3:95489497-95489516 | None:intergenic | 45.0% | |
| CAGATGAAGACGATGCTGAT+TGG | + | chr3.3:95491010-95491029 | MS.gene063114:CDS | 45.0% | |
| CTGTCATGTAAAGCTGTGGT+TGG | + | chr3.3:95489736-95489755 | MS.gene063114:intron | 45.0% | |
| GAAGATGAAGACCCTGAAGA+AGG | + | chr3.3:95491033-95491052 | MS.gene063114:CDS | 45.0% | |
| GAGGTAAGAATCAGCACCTA+AGG | - | chr3.3:95489455-95489474 | None:intergenic | 45.0% | |
| GATTTCAACGTCGGTGATAG+TGG | - | chr3.3:95489488-95489507 | None:intergenic | 45.0% | |
| GCGGTCAAGAAACTAAGAGA+TGG | - | chr3.3:95490062-95490081 | None:intergenic | 45.0% | |
| GGCGGCGAAAATGATAGAAT+AGG | - | chr3.3:95489541-95489560 | None:intergenic | 45.0% | |
| GTTCTTCCTGAAGCTTCCTT+AGG | + | chr3.3:95489436-95489455 | MS.gene063114:CDS | 45.0% | |
| TTCCACCCATTCAAACTTCC+AGG | + | chr3.3:95490876-95490895 | MS.gene063114:intron | 45.0% | |
| TTGTGGGTTTCACATCACTG+TGG | + | chr3.3:95490565-95490584 | MS.gene063114:intron | 45.0% | |
| ! | AACTCCCGAGATACGCTTTT+TGG | + | chr3.3:95489910-95489929 | MS.gene063114:CDS | 45.0% |
| ! | TCCTCCAAAAAGCGTATCTC+GGG | - | chr3.3:95489917-95489936 | None:intergenic | 45.0% |
| !! | AACAATGCTTTTGAGGGGAG+AGG | + | chr3.3:95490490-95490509 | MS.gene063114:intron | 45.0% |
| !! | ACAATGCTTTTGAGGGGAGA+GGG | + | chr3.3:95490491-95490510 | MS.gene063114:intron | 45.0% |
| ACTAGGCCCTTGTTGCTATG+TGG | + | chr3.3:95490369-95490388 | MS.gene063114:intron | 50.0% | |
| ATAATTGTGGGGAAACCGGC+AGG | - | chr3.3:95490337-95490356 | None:intergenic | 50.0% | |
| CTAGGCCCTTGTTGCTATGT+GGG | + | chr3.3:95490370-95490389 | MS.gene063114:intron | 50.0% | |
| GACCTGGAAGTTTGAATGGG+TGG | - | chr3.3:95490881-95490900 | None:intergenic | 50.0% | |
| TCAGCACCTAAGGAAGCTTC+AGG | - | chr3.3:95489445-95489464 | None:intergenic | 50.0% | |
| !! | AACGTCGGTGATAGTGGTGA+CGG | - | chr3.3:95489482-95489501 | None:intergenic | 50.0% |
| !! | GCATAAGTGAGCACTGGCTA+TGG | - | chr3.3:95490771-95490790 | None:intergenic | 50.0% |
| !! | TCCCGAGATACGCTTTTTGG+AGG | + | chr3.3:95489913-95489932 | MS.gene063114:CDS | 50.0% |
| !! | TGATAGTGGTGACGGAAGAG+AGG | - | chr3.3:95489474-95489493 | None:intergenic | 50.0% |
| AAGAGGGAAGGAAGTGGCCA+TGG | + | chr3.3:95489823-95489842 | MS.gene063114:CDS | 55.0% | |
| GGGAAACCGGCAGGAAATCA+CGG | - | chr3.3:95490328-95490347 | None:intergenic | 55.0% | |
| TGGCGTGCATAAGTGAGCAC+TGG | - | chr3.3:95490777-95490796 | None:intergenic | 55.0% | |
| TGGTCTGGAAAGGGCCAAAG+TGG | - | chr3.3:95490042-95490061 | None:intergenic | 55.0% | |
| ! | ATGTTCGCAGTGAGTTGGGG+AGG | + | chr3.3:95489870-95489889 | MS.gene063114:CDS | 55.0% |
| ! | TGGGGAGGCGTATGAAGTTG+CGG | + | chr3.3:95489885-95489904 | MS.gene063114:CDS | 55.0% |
| !! | ATGCTTTTGAGGGGAGAGGG+TGG | + | chr3.3:95490494-95490513 | MS.gene063114:intron | 55.0% |
| !! | TGCTTTTGAGGGGAGAGGGT+GGG | + | chr3.3:95490495-95490514 | MS.gene063114:intron | 55.0% |
| GGAAGGAAGTGGCCATGGCT+GGG | + | chr3.3:95489828-95489847 | MS.gene063114:CDS | 60.0% | |
| ! | AGGGGAGAGGGTGGGTTCTT+TGG | + | chr3.3:95490503-95490522 | MS.gene063114:intron | 60.0% |
| GGGAAGGAAGTGGCCATGGC+TGG | + | chr3.3:95489827-95489846 | MS.gene063114:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 95489367 | 95491074 | 95489367 | ID=MS.gene063114 |
| chr3.3 | mRNA | 95489367 | 95491074 | 95489367 | ID=MS.gene063114.t1;Parent=MS.gene063114 |
| chr3.3 | exon | 95489367 | 95489522 | 95489367 | ID=MS.gene063114.t1.exon1;Parent=MS.gene063114.t1 |
| chr3.3 | CDS | 95489367 | 95489522 | 95489367 | ID=cds.MS.gene063114.t1;Parent=MS.gene063114.t1 |
| chr3.3 | exon | 95489788 | 95489961 | 95489788 | ID=MS.gene063114.t1.exon2;Parent=MS.gene063114.t1 |
| chr3.3 | CDS | 95489788 | 95489961 | 95489788 | ID=cds.MS.gene063114.t1;Parent=MS.gene063114.t1 |
| chr3.3 | exon | 95490898 | 95491074 | 95490898 | ID=MS.gene063114.t1.exon3;Parent=MS.gene063114.t1 |
| chr3.3 | CDS | 95490898 | 95491074 | 95490898 | ID=cds.MS.gene063114.t1;Parent=MS.gene063114.t1 |
| Gene Sequence |
| Protein sequence |
