Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68764.t1 | XP_003604015.1 | 89 | 237 | 15 | 4 | 1 | 234 | 1 | 229 | 7.30E-91 | 343.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68764.t1 | O65693 | 69.8 | 179 | 40 | 2 | 56 | 234 | 51 | 215 | 5.3e-58 | 225.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68764.t1 | G7J7U0 | 89.0 | 237 | 15 | 4 | 1 | 234 | 1 | 229 | 5.3e-91 | 343.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049149 | MS.gene68764 | 0.801349 | 9.39E-49 | -1.69E-46 |
| MS.gene049150 | MS.gene68764 | 0.862796 | 3.93E-64 | -1.69E-46 |
| MS.gene049151 | MS.gene68764 | 0.815119 | 1.09E-51 | -1.69E-46 |
| MS.gene049153 | MS.gene68764 | 0.871347 | 7.33E-67 | -1.69E-46 |
| MS.gene049195 | MS.gene68764 | 0.816301 | 5.94E-52 | -1.69E-46 |
| MS.gene049233 | MS.gene68764 | 0.818252 | 2.16E-52 | -1.69E-46 |
| MS.gene049260 | MS.gene68764 | 0.847285 | 1.27E-59 | -1.69E-46 |
| MS.gene049297 | MS.gene68764 | 0.840861 | 6.74E-58 | -1.69E-46 |
| MS.gene04933 | MS.gene68764 | 0.845916 | 3.01E-59 | -1.69E-46 |
| MS.gene049346 | MS.gene68764 | 0.819829 | 9.45E-53 | -1.69E-46 |
| MS.gene049351 | MS.gene68764 | 0.818703 | 1.71E-52 | -1.69E-46 |
| MS.gene049378 | MS.gene68764 | 0.855167 | 7.56E-62 | -1.69E-46 |
| MS.gene049426 | MS.gene68764 | 0.809591 | 1.75E-50 | -1.69E-46 |
| MS.gene049599 | MS.gene68764 | -0.818035 | 2.42E-52 | -1.69E-46 |
| MS.gene049758 | MS.gene68764 | 0.908792 | 1.16E-81 | -1.69E-46 |
| MS.gene049910 | MS.gene68764 | 0.863694 | 2.07E-64 | -1.69E-46 |
| MS.gene049915 | MS.gene68764 | 0.83176 | 1.39E-55 | -1.69E-46 |
| MS.gene049993 | MS.gene68764 | 0.805448 | 1.33E-49 | -1.69E-46 |
| MS.gene050099 | MS.gene68764 | 0.819369 | 1.20E-52 | -1.69E-46 |
| MS.gene050128 | MS.gene68764 | 0.813376 | 2.64E-51 | -1.69E-46 |
| MS.gene050168 | MS.gene68764 | 0.807279 | 5.46E-50 | -1.69E-46 |
| MS.gene050226 | MS.gene68764 | 0.858278 | 9.20E-63 | -1.69E-46 |
| MS.gene050227 | MS.gene68764 | 0.861869 | 7.58E-64 | -1.69E-46 |
| MS.gene050228 | MS.gene68764 | 0.85858 | 7.47E-63 | -1.69E-46 |
| MS.gene050301 | MS.gene68764 | 0.878565 | 2.53E-69 | -1.69E-46 |
| MS.gene050319 | MS.gene68764 | -0.847236 | 1.31E-59 | -1.69E-46 |
| MS.gene050330 | MS.gene68764 | -0.85872 | 6.79E-63 | -1.69E-46 |
| MS.gene050397 | MS.gene68764 | 0.826419 | 2.74E-54 | -1.69E-46 |
| MS.gene050438 | MS.gene68764 | 0.83139 | 1.72E-55 | -1.69E-46 |
| MS.gene050442 | MS.gene68764 | 0.802151 | 6.43E-49 | -1.69E-46 |
| MS.gene050464 | MS.gene68764 | 0.850202 | 1.98E-60 | -1.69E-46 |
| MS.gene050516 | MS.gene68764 | 0.806671 | 7.34E-50 | -1.69E-46 |
| MS.gene050529 | MS.gene68764 | 0.810904 | 9.14E-51 | -1.69E-46 |
| MS.gene050558 | MS.gene68764 | 0.883057 | 6.19E-71 | -1.69E-46 |
| MS.gene050560 | MS.gene68764 | 0.820456 | 6.79E-53 | -1.69E-46 |
| MS.gene050623 | MS.gene68764 | -0.855646 | 5.49E-62 | -1.69E-46 |
| MS.gene050642 | MS.gene68764 | 0.874681 | 5.59E-68 | -1.69E-46 |
| MS.gene050650 | MS.gene68764 | 0.809667 | 1.69E-50 | -1.69E-46 |
| MS.gene050670 | MS.gene68764 | 0.818237 | 2.18E-52 | -1.69E-46 |
| MS.gene050719 | MS.gene68764 | 0.824691 | 7.04E-54 | -1.69E-46 |
| MS.gene050721 | MS.gene68764 | 0.823238 | 1.54E-53 | -1.69E-46 |
| MS.gene050724 | MS.gene68764 | 0.821422 | 4.07E-53 | -1.69E-46 |
| MS.gene050787 | MS.gene68764 | 0.839038 | 2.01E-57 | -1.69E-46 |
| MS.gene050820 | MS.gene68764 | 0.842251 | 2.90E-58 | -1.69E-46 |
| MS.gene05101 | MS.gene68764 | 0.815914 | 7.24E-52 | -1.69E-46 |
| MS.gene05119 | MS.gene68764 | -0.869818 | 2.33E-66 | -1.69E-46 |
| MS.gene051257 | MS.gene68764 | 0.800739 | 1.25E-48 | -1.69E-46 |
| MS.gene051471 | MS.gene68764 | 0.814281 | 1.67E-51 | -1.69E-46 |
| MS.gene051582 | MS.gene68764 | 0.836638 | 8.32E-57 | -1.69E-46 |
| MS.gene051662 | MS.gene68764 | 0.849834 | 2.51E-60 | -1.69E-46 |
| MS.gene051663 | MS.gene68764 | 0.866583 | 2.56E-65 | -1.69E-46 |
| MS.gene051860 | MS.gene68764 | 0.902951 | 5.73E-79 | -1.69E-46 |
| MS.gene051983 | MS.gene68764 | 0.87957 | 1.12E-69 | -1.69E-46 |
| MS.gene052011 | MS.gene68764 | -0.827195 | 1.79E-54 | -1.69E-46 |
| MS.gene052077 | MS.gene68764 | 0.859836 | 3.14E-63 | -1.69E-46 |
| MS.gene052078 | MS.gene68764 | 0.840198 | 1.00E-57 | -1.69E-46 |
| MS.gene052176 | MS.gene68764 | 0.823381 | 1.43E-53 | -1.69E-46 |
| MS.gene052178 | MS.gene68764 | 0.838021 | 3.68E-57 | -1.69E-46 |
| MS.gene052188 | MS.gene68764 | 0.841432 | 4.77E-58 | -1.69E-46 |
| MS.gene052207 | MS.gene68764 | 0.83307 | 6.58E-56 | -1.69E-46 |
| MS.gene052253 | MS.gene68764 | 0.851062 | 1.13E-60 | -1.69E-46 |
| MS.gene052255 | MS.gene68764 | 0.82785 | 1.25E-54 | -1.69E-46 |
| MS.gene052309 | MS.gene68764 | -0.816977 | 4.19E-52 | -1.69E-46 |
| MS.gene052310 | MS.gene68764 | 0.808472 | 3.05E-50 | -1.69E-46 |
| MS.gene052340 | MS.gene68764 | 0.839789 | 1.28E-57 | -1.69E-46 |
| MS.gene052389 | MS.gene68764 | 0.803697 | 3.08E-49 | -1.69E-46 |
| MS.gene052600 | MS.gene68764 | 0.817311 | 3.52E-52 | -1.69E-46 |
| MS.gene05316 | MS.gene68764 | 0.806397 | 8.39E-50 | -1.69E-46 |
| MS.gene053184 | MS.gene68764 | 0.824277 | 8.81E-54 | -1.69E-46 |
| MS.gene053209 | MS.gene68764 | 0.80442 | 2.18E-49 | -1.69E-46 |
| MS.gene053557 | MS.gene68764 | 0.81001 | 1.43E-50 | -1.69E-46 |
| MS.gene053836 | MS.gene68764 | 0.928036 | 5.09E-92 | -1.69E-46 |
| MS.gene053907 | MS.gene68764 | 0.805649 | 1.21E-49 | -1.69E-46 |
| MS.gene053928 | MS.gene68764 | 0.820617 | 6.24E-53 | -1.69E-46 |
| MS.gene054010 | MS.gene68764 | 0.805802 | 1.12E-49 | -1.69E-46 |
| MS.gene054011 | MS.gene68764 | 0.836992 | 6.76E-57 | -1.69E-46 |
| MS.gene054012 | MS.gene68764 | 0.809099 | 2.24E-50 | -1.69E-46 |
| MS.gene054123 | MS.gene68764 | 0.803088 | 4.12E-49 | -1.69E-46 |
| MS.gene054183 | MS.gene68764 | 0.845092 | 5.04E-59 | -1.69E-46 |
| MS.gene054184 | MS.gene68764 | 0.816248 | 6.10E-52 | -1.69E-46 |
| MS.gene054185 | MS.gene68764 | 0.817302 | 3.54E-52 | -1.69E-46 |
| MS.gene054379 | MS.gene68764 | 0.811175 | 7.99E-51 | -1.69E-46 |
| MS.gene054473 | MS.gene68764 | 0.877159 | 7.86E-69 | -1.69E-46 |
| MS.gene054542 | MS.gene68764 | 0.820412 | 6.96E-53 | -1.69E-46 |
| MS.gene054667 | MS.gene68764 | 0.817945 | 2.53E-52 | -1.69E-46 |
| MS.gene054918 | MS.gene68764 | 0.820973 | 5.17E-53 | -1.69E-46 |
| MS.gene054930 | MS.gene68764 | 0.877728 | 4.98E-69 | -1.69E-46 |
| MS.gene054942 | MS.gene68764 | 0.863714 | 2.04E-64 | -1.69E-46 |
| MS.gene054983 | MS.gene68764 | 0.857258 | 1.85E-62 | -1.69E-46 |
| MS.gene055116 | MS.gene68764 | 0.828687 | 7.83E-55 | -1.69E-46 |
| MS.gene055277 | MS.gene68764 | 0.857075 | 2.09E-62 | -1.69E-46 |
| MS.gene055356 | MS.gene68764 | 0.818552 | 1.85E-52 | -1.69E-46 |
| MS.gene055358 | MS.gene68764 | 0.832793 | 7.72E-56 | -1.69E-46 |
| MS.gene055390 | MS.gene68764 | 0.83196 | 1.24E-55 | -1.69E-46 |
| MS.gene055422 | MS.gene68764 | 0.905328 | 4.82E-80 | -1.69E-46 |
| MS.gene055428 | MS.gene68764 | 0.872189 | 3.85E-67 | -1.69E-46 |
| MS.gene05553 | MS.gene68764 | 0.824525 | 7.70E-54 | -1.69E-46 |
| MS.gene055604 | MS.gene68764 | 0.850694 | 1.44E-60 | -1.69E-46 |
| MS.gene055712 | MS.gene68764 | 0.875939 | 2.07E-68 | -1.69E-46 |
| MS.gene055859 | MS.gene68764 | 0.816922 | 4.31E-52 | -1.69E-46 |
| MS.gene055924 | MS.gene68764 | 0.811713 | 6.10E-51 | -1.69E-46 |
| MS.gene056069 | MS.gene68764 | -0.820612 | 6.26E-53 | -1.69E-46 |
| MS.gene056407 | MS.gene68764 | 0.824022 | 1.01E-53 | -1.69E-46 |
| MS.gene056612 | MS.gene68764 | 0.842098 | 3.18E-58 | -1.69E-46 |
| MS.gene056659 | MS.gene68764 | 0.806482 | 8.05E-50 | -1.69E-46 |
| MS.gene056723 | MS.gene68764 | 0.806664 | 7.37E-50 | -1.69E-46 |
| MS.gene056875 | MS.gene68764 | 0.851194 | 1.04E-60 | -1.69E-46 |
| MS.gene056899 | MS.gene68764 | -0.823763 | 1.16E-53 | -1.69E-46 |
| MS.gene056979 | MS.gene68764 | 0.883607 | 3.89E-71 | -1.69E-46 |
| MS.gene05702 | MS.gene68764 | 0.856812 | 2.50E-62 | -1.69E-46 |
| MS.gene057121 | MS.gene68764 | 0.881854 | 1.70E-70 | -1.69E-46 |
| MS.gene057200 | MS.gene68764 | 0.813853 | 2.07E-51 | -1.69E-46 |
| MS.gene057245 | MS.gene68764 | 0.867142 | 1.70E-65 | -1.69E-46 |
| MS.gene057260 | MS.gene68764 | 0.831116 | 2.00E-55 | -1.69E-46 |
| MS.gene057439 | MS.gene68764 | 0.874492 | 6.47E-68 | -1.69E-46 |
| MS.gene057448 | MS.gene68764 | 0.848567 | 5.64E-60 | -1.69E-46 |
| MS.gene057449 | MS.gene68764 | 0.829425 | 5.18E-55 | -1.69E-46 |
| MS.gene057450 | MS.gene68764 | 0.850195 | 1.99E-60 | -1.69E-46 |
| MS.gene057469 | MS.gene68764 | 0.860157 | 2.51E-63 | -1.69E-46 |
| MS.gene057750 | MS.gene68764 | 0.82333 | 1.47E-53 | -1.69E-46 |
| MS.gene057754 | MS.gene68764 | 0.821223 | 4.53E-53 | -1.69E-46 |
| MS.gene058002 | MS.gene68764 | 0.892002 | 2.37E-74 | -1.69E-46 |
| MS.gene05811 | MS.gene68764 | 0.883906 | 3.02E-71 | -1.69E-46 |
| MS.gene058139 | MS.gene68764 | 0.81294 | 3.29E-51 | -1.69E-46 |
| MS.gene058142 | MS.gene68764 | 0.868512 | 6.18E-66 | -1.69E-46 |
| MS.gene058146 | MS.gene68764 | 0.801619 | 8.27E-49 | -1.69E-46 |
| MS.gene058168 | MS.gene68764 | 0.80004 | 1.74E-48 | -1.69E-46 |
| MS.gene058276 | MS.gene68764 | 0.827997 | 1.15E-54 | -1.69E-46 |
| MS.gene058660 | MS.gene68764 | 0.819502 | 1.12E-52 | -1.69E-46 |
| MS.gene058710 | MS.gene68764 | 0.845599 | 3.67E-59 | -1.69E-46 |
| MS.gene058749 | MS.gene68764 | 0.833283 | 5.83E-56 | -1.69E-46 |
| MS.gene058833 | MS.gene68764 | 0.823667 | 1.22E-53 | -1.69E-46 |
| MS.gene058845 | MS.gene68764 | 0.83815 | 3.41E-57 | -1.69E-46 |
| MS.gene05886 | MS.gene68764 | 0.892559 | 1.42E-74 | -1.69E-46 |
| MS.gene059033 | MS.gene68764 | -0.814303 | 1.65E-51 | -1.69E-46 |
| MS.gene05907 | MS.gene68764 | 0.878209 | 3.38E-69 | -1.69E-46 |
| MS.gene059124 | MS.gene68764 | 0.818377 | 2.02E-52 | -1.69E-46 |
| MS.gene059128 | MS.gene68764 | 0.819745 | 9.88E-53 | -1.69E-46 |
| MS.gene059247 | MS.gene68764 | 0.845115 | 4.97E-59 | -1.69E-46 |
| MS.gene059285 | MS.gene68764 | 0.862311 | 5.55E-64 | -1.69E-46 |
| MS.gene059294 | MS.gene68764 | 0.806587 | 7.65E-50 | -1.69E-46 |
| MS.gene059420 | MS.gene68764 | 0.828307 | 9.67E-55 | -1.69E-46 |
| MS.gene059421 | MS.gene68764 | 0.810159 | 1.32E-50 | -1.69E-46 |
| MS.gene059518 | MS.gene68764 | 0.86422 | 1.42E-64 | -1.69E-46 |
| MS.gene059597 | MS.gene68764 | 0.830666 | 2.58E-55 | -1.69E-46 |
| MS.gene059609 | MS.gene68764 | 0.809779 | 1.60E-50 | -1.69E-46 |
| MS.gene059676 | MS.gene68764 | 0.800023 | 1.75E-48 | -1.69E-46 |
| MS.gene059688 | MS.gene68764 | 0.821851 | 3.24E-53 | -1.69E-46 |
| MS.gene059692 | MS.gene68764 | 0.824535 | 7.66E-54 | -1.69E-46 |
| MS.gene059695 | MS.gene68764 | 0.813986 | 1.94E-51 | -1.69E-46 |
| MS.gene05984 | MS.gene68764 | 0.903934 | 2.08E-79 | -1.69E-46 |
| MS.gene059894 | MS.gene68764 | 0.862001 | 6.90E-64 | -1.69E-46 |
| MS.gene059899 | MS.gene68764 | 0.846415 | 2.20E-59 | -1.69E-46 |
| MS.gene060137 | MS.gene68764 | -0.803691 | 3.09E-49 | -1.69E-46 |
| MS.gene06037 | MS.gene68764 | 0.847807 | 9.15E-60 | -1.69E-46 |
| MS.gene060404 | MS.gene68764 | 0.811342 | 7.35E-51 | -1.69E-46 |
| MS.gene060476 | MS.gene68764 | 0.824729 | 6.89E-54 | -1.69E-46 |
| MS.gene060480 | MS.gene68764 | 0.818906 | 1.53E-52 | -1.69E-46 |
| MS.gene060495 | MS.gene68764 | 0.802962 | 4.37E-49 | -1.69E-46 |
| MS.gene060683 | MS.gene68764 | 0.822323 | 2.52E-53 | -1.69E-46 |
| MS.gene060685 | MS.gene68764 | 0.806423 | 8.29E-50 | -1.69E-46 |
| MS.gene060994 | MS.gene68764 | 0.839408 | 1.61E-57 | -1.69E-46 |
| MS.gene061000 | MS.gene68764 | 0.830788 | 2.41E-55 | -1.69E-46 |
| MS.gene061040 | MS.gene68764 | -0.813593 | 2.37E-51 | -1.69E-46 |
| MS.gene061049 | MS.gene68764 | 0.882712 | 8.28E-71 | -1.69E-46 |
| MS.gene061085 | MS.gene68764 | 0.813003 | 3.19E-51 | -1.69E-46 |
| MS.gene061114 | MS.gene68764 | -0.832398 | 9.67E-56 | -1.69E-46 |
| MS.gene061159 | MS.gene68764 | 0.846819 | 1.71E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68764.t1 | MTR_3g117600 | 90.717 | 237 | 11 | 4 | 1 | 234 | 1 | 229 | 5.23e-137 | 384 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68764.t1 | AT4G34730 | 59.825 | 229 | 75 | 3 | 6 | 234 | 4 | 215 | 1.94e-86 | 255 |
| MS.gene68764.t1 | AT4G34730 | 59.825 | 229 | 75 | 3 | 6 | 234 | 4 | 215 | 1.94e-86 | 255 |
| MS.gene68764.t1 | AT4G34730 | 59.825 | 229 | 75 | 3 | 6 | 234 | 4 | 215 | 1.94e-86 | 255 |
Find 49 sgRNAs with CRISPR-Local
Find 139 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACTCACTGCGAACATATT+TGG | 0.174334 | 3.2:+92846374 | None:intergenic |
| TGAAATCACCGCTGATTTAC+AGG | 0.294810 | 3.2:-92846734 | MS.gene68764:intron |
| AAATATGTTCGCAGTGAGTT+GGG | 0.366464 | 3.2:-92846372 | MS.gene68764:CDS |
| GGCAAATTGCAAGACGTTAT+CGG | 0.368809 | 3.2:+92846822 | None:intergenic |
| GGGAAGGAAGTGGCCATGGC+TGG | 0.375787 | 3.2:-92846411 | MS.gene68764:CDS |
| GTTCTTCCTGAAGCTTCCTT+AGG | 0.384742 | 3.2:-92846799 | MS.gene68764:CDS |
| AAGTTCTCTCCGAATTTGTT+TGG | 0.408703 | 3.2:+92846861 | None:intergenic |
| TCAGCACCTAAGGAAGCTTC+AGG | 0.415676 | 3.2:+92846793 | None:intergenic |
| ACATAAATGATACCTTCTTC+AGG | 0.417511 | 3.2:+92845250 | None:intergenic |
| GAATCCAAGGAGAGTCAAAA+TGG | 0.420166 | 3.2:-92846887 | MS.gene68764:CDS |
| AAATGGTTGCCAAACAAATT+CGG | 0.421978 | 3.2:-92846870 | MS.gene68764:CDS |
| CAGATGAAGACGATGCTGAT+TGG | 0.429266 | 3.2:-92845285 | MS.gene68764:CDS |
| AGATGAAGACGATGCTGATT+GGG | 0.431019 | 3.2:-92845284 | MS.gene68764:CDS |
| GAGGTAAGAATCAGCACCTA+AGG | 0.457882 | 3.2:+92846783 | None:intergenic |
| CGGTCACACTCACACCTTGT+TGG | 0.460357 | 3.2:-92847020 | MS.gene68764:CDS |
| AATAATGAGGATAAAGTGTA+TGG | 0.468522 | 3.2:-92846911 | MS.gene68764:CDS |
| CAAATATGTTCGCAGTGAGT+TGG | 0.480055 | 3.2:-92846373 | MS.gene68764:CDS |
| CATAAATGATACCTTCTTCA+GGG | 0.510113 | 3.2:+92845251 | None:intergenic |
| TGAAGAAGGTATCATTTATG+TGG | 0.517227 | 3.2:-92845248 | MS.gene68764:CDS |
| CTGAAATATAGTTGCAGTTG+CGG | 0.522736 | 3.2:+92846978 | None:intergenic |
| GGTGATTTCAACGTCGGTGA+TGG | 0.525546 | 3.2:+92846747 | None:intergenic |
| GGAAGGAAGTGGCCATGGCT+GGG | 0.525786 | 3.2:-92846410 | MS.gene68764:CDS |
| TTCAGACTCAGAGGCGTGGG+TGG | 0.556216 | 3.2:-92846960 | MS.gene68764:CDS |
| AGGCGTGGGTGGATCCTCAC+AGG | 0.564558 | 3.2:-92846949 | MS.gene68764:CDS |
| AGATGAAAGAGGGAAGGAAG+TGG | 0.566160 | 3.2:-92846421 | MS.gene68764:CDS |
| GAAGATGAAGACCCTGAAGA+AGG | 0.579729 | 3.2:-92845262 | MS.gene68764:CDS |
| ATGCTTCAAGAATAATAATG+AGG | 0.581339 | 3.2:-92846924 | MS.gene68764:CDS |
| AAGAGGGAAGGAAGTGGCCA+TGG | 0.582663 | 3.2:-92846415 | MS.gene68764:CDS |
| AACGTCGGTGATGGTGGTGA+CGG | 0.583949 | 3.2:+92846756 | None:intergenic |
| CGGTTATTACATCGCCAACA+AGG | 0.589245 | 3.2:+92847006 | None:intergenic |
| TGGGGAGGCGTATGAAGTTG+CGG | 0.591668 | 3.2:-92846353 | MS.gene68764:CDS |
| TATTTCAGACTCAGAGGCGT+GGG | 0.594674 | 3.2:-92846963 | MS.gene68764:CDS |
| TAAAGTGTATGGCGAATCCA+AGG | 0.594849 | 3.2:-92846900 | MS.gene68764:CDS |
| AATATGTTCGCAGTGAGTTG+GGG | 0.594957 | 3.2:-92846371 | MS.gene68764:CDS |
| TAAGGAAGCTTCAGGAAGAA+CGG | 0.601825 | 3.2:+92846801 | None:intergenic |
| ATGTTCGCAGTGAGTTGGGG+AGG | 0.601873 | 3.2:-92846368 | MS.gene68764:CDS |
| TTCTTGAAGCATTTCCTGTG+AGG | 0.605345 | 3.2:+92846935 | None:intergenic |
| GATTTCAACGTCGGTGATGG+TGG | 0.617438 | 3.2:+92846750 | None:intergenic |
| TAGTTGCAGTTGCGGTTGTG+CGG | 0.617602 | 3.2:+92846986 | None:intergenic |
| TGATGGTGGTGACGGAAGAG+AGG | 0.626472 | 3.2:+92846764 | None:intergenic |
| GACGCAATTGCTTCACACCG+CGG | 0.639535 | 3.2:-92847058 | MS.gene68764:CDS |
| CGATGGTATCAGTGCAGCCG+CGG | 0.642361 | 3.2:+92847041 | None:intergenic |
| CAGTTGGATTCATCAGTGAA+CGG | 0.643319 | 3.2:-92845337 | MS.gene68764:CDS |
| ATATTTCAGACTCAGAGGCG+TGG | 0.649311 | 3.2:-92846964 | MS.gene68764:CDS |
| CGCGGCTGCACTGATACCAT+CGG | 0.650425 | 3.2:-92847040 | MS.gene68764:CDS |
| ATCAGCGGTGATTTCAACGT+CGG | 0.653697 | 3.2:+92846741 | None:intergenic |
| CAACTATATTTCAGACTCAG+AGG | 0.659888 | 3.2:-92846969 | MS.gene68764:CDS |
| AATAAGAACCTGTAAATCAG+CGG | 0.693763 | 3.2:+92846726 | None:intergenic |
| CAAGGTGTGAGTGTGACCGA+TGG | 0.717093 | 3.2:+92847024 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACATATAGAAAAAAATATA+AGG | + | chr3.2:92846244-92846263 | None:intergenic | 10.0% |
| !! | ATTAAATGAGAAAAATGAAA+AGG | + | chr3.2:92845744-92845763 | None:intergenic | 15.0% |
| !! | TGCTTAAATATATAAAAGAA+TGG | + | chr3.2:92846811-92846830 | None:intergenic | 15.0% |
| !!! | CATATTCGTTAATTTTAAAA+AGG | - | chr3.2:92846674-92846693 | MS.gene68764:intron | 15.0% |
| !! | AAGATAAAAAAAGATTTCAG+CGG | + | chr3.2:92846647-92846666 | None:intergenic | 20.0% |
| !! | GAATTATCACACTATAAATT+CGG | + | chr3.2:92845636-92845655 | None:intergenic | 20.0% |
| !! | GTCATGAAACAAAATAAATA+AGG | + | chr3.2:92845788-92845807 | None:intergenic | 20.0% |
| !! | TCATCAGGAAAATAAAAATA+AGG | + | chr3.2:92846554-92846573 | None:intergenic | 20.0% |
| !!! | ATATTTACCGTTTCTTTTTA+AGG | - | chr3.2:92846045-92846064 | MS.gene68764:intron | 20.0% |
| ! | AATAATGAGGATAAAGTGTA+TGG | - | chr3.2:92845398-92845417 | MS.gene68764:CDS | 25.0% |
| ! | AGTGATACATAAGAATAAGA+TGG | - | chr3.2:92846432-92846451 | MS.gene68764:CDS | 25.0% |
| ! | ATGCTTCAAGAATAATAATG+AGG | - | chr3.2:92845385-92845404 | MS.gene68764:CDS | 25.0% |
| ! | CAAAGAACCTTAAAAAGAAA+CGG | + | chr3.2:92846055-92846074 | None:intergenic | 25.0% |
| ! | CTCATTTAATCTAGTGTATT+TGG | - | chr3.2:92845754-92845773 | MS.gene68764:intron | 25.0% |
| ! | TATATATTTAAGCAGCTTCT+TGG | - | chr3.2:92846817-92846836 | MS.gene68764:CDS | 25.0% |
| ! | TCTATCTACCATATATATGT+TGG | - | chr3.2:92846292-92846311 | MS.gene68764:intron | 25.0% |
| ! | TTTATAGTGTGATAATTCAG+TGG | - | chr3.2:92845638-92845657 | MS.gene68764:intron | 25.0% |
| !! | TATTCAAACAATGCTTTTGA+GGG | - | chr3.2:92846498-92846517 | MS.gene68764:intron | 25.0% |
| AAATGGTTGCCAAACAAATT+CGG | - | chr3.2:92845439-92845458 | MS.gene68764:intron | 30.0% | |
| AAGAACCATCTCATAATTGT+GGG | + | chr3.2:92846363-92846382 | None:intergenic | 30.0% | |
| AATAAGAACCTGTAAATCAG+CGG | + | chr3.2:92845586-92845605 | None:intergenic | 30.0% | |
| ACATAAATGATACCTTCTTC+AGG | + | chr3.2:92847062-92847081 | None:intergenic | 30.0% | |
| AGAAAAATGAAAAGGCAATG+TGG | + | chr3.2:92845736-92845755 | None:intergenic | 30.0% | |
| ATTATGAGATGGTTCTTACT+AGG | - | chr3.2:92846366-92846385 | MS.gene68764:CDS | 30.0% | |
| CATAAATGATACCTTCTTCA+GGG | + | chr3.2:92847061-92847080 | None:intergenic | 30.0% | |
| TAAGAACCATCTCATAATTG+TGG | + | chr3.2:92846364-92846383 | None:intergenic | 30.0% | |
| TATCTAATATTGCAATGACC+TGG | + | chr3.2:92846911-92846930 | None:intergenic | 30.0% | |
| TTATCACACTATAAATTCGG+CGG | + | chr3.2:92845633-92845652 | None:intergenic | 30.0% | |
| ! | AAGTTTGTGTTGTTCTGTTT+CGG | - | chr3.2:92846087-92846106 | MS.gene68764:intron | 30.0% |
| ! | ATTCAAACAATGCTTTTGAG+GGG | - | chr3.2:92846499-92846518 | MS.gene68764:intron | 30.0% |
| ! | CTATTCAAACAATGCTTTTG+AGG | - | chr3.2:92846497-92846516 | MS.gene68764:intron | 30.0% |
| ! | TAACTCAACACTTTTAGTGT+AGG | - | chr3.2:92845837-92845856 | MS.gene68764:intron | 30.0% |
| ! | TTTCCTGATGATGAAATTGT+GGG | - | chr3.2:92846563-92846582 | MS.gene68764:intron | 30.0% |
| !! | TGAAGAAGGTATCATTTATG+TGG | - | chr3.2:92847061-92847080 | MS.gene68764:CDS | 30.0% |
| !! | TTTTCCTGATGATGAAATTG+TGG | - | chr3.2:92846562-92846581 | MS.gene68764:intron | 30.0% |
| AAACCCACAATTTCATCATC+AGG | + | chr3.2:92846569-92846588 | None:intergenic | 35.0% | |
| AAATATGTTCGCAGTGAGTT+GGG | - | chr3.2:92845937-92845956 | MS.gene68764:intron | 35.0% | |
| AATGACCTGGAAGTTTGAAT+GGG | + | chr3.2:92846898-92846917 | None:intergenic | 35.0% | |
| ACATGAGTGAAAATTTGTGC+TGG | + | chr3.2:92846157-92846176 | None:intergenic | 35.0% | |
| AGAACCATCTCATAATTGTG+GGG | + | chr3.2:92846362-92846381 | None:intergenic | 35.0% | |
| ATTAACCCACATAGCAACAA+GGG | + | chr3.2:92846392-92846411 | None:intergenic | 35.0% | |
| CAACTATATTTCAGACTCAG+AGG | - | chr3.2:92845340-92845359 | MS.gene68764:CDS | 35.0% | |
| CAAGAAATTGTCCATACAAG+AGG | + | chr3.2:92846475-92846494 | None:intergenic | 35.0% | |
| CATGAGTGAAAATTTGTGCT+GGG | + | chr3.2:92846156-92846175 | None:intergenic | 35.0% | |
| CTAGCAGTGCATTATATCAA+TGG | - | chr3.2:92845699-92845718 | MS.gene68764:intron | 35.0% | |
| CTGAAATATAGTTGCAGTTG+CGG | + | chr3.2:92845334-92845353 | None:intergenic | 35.0% | |
| GAGTGAAACAAGCAAAAACA+TGG | - | chr3.2:92846947-92846966 | MS.gene68764:CDS | 35.0% | |
| GTTGTGAGCTGTTATTGATA+TGG | - | chr3.2:92846864-92846883 | MS.gene68764:CDS | 35.0% | |
| TTAGTTCATCACTACGCATA+GGG | - | chr3.2:92846262-92846281 | MS.gene68764:intron | 35.0% | |
| ! | AAGTTCTCTCCGAATTTGTT+TGG | + | chr3.2:92845451-92845470 | None:intergenic | 35.0% |
| ! | ACTTTTAGTGTAGGTCGTTA+GGG | - | chr3.2:92845846-92845865 | MS.gene68764:intron | 35.0% |
| ! | AGGGTGTATGTATCTGTTTT+TGG | - | chr3.2:92845865-92845884 | MS.gene68764:intron | 35.0% |
| !!! | CTGTTTTTGGAGATGAAAGA+GGG | - | chr3.2:92845878-92845897 | MS.gene68764:intron | 35.0% |
| !!! | TCTGTTTTTGGAGATGAAAG+AGG | - | chr3.2:92845877-92845896 | MS.gene68764:intron | 35.0% |
| AAGCAAAAACATGGAGCAGT+TGG | - | chr3.2:92846956-92846975 | MS.gene68764:CDS | 40.0% | |
| AATATGTTCGCAGTGAGTTG+GGG | - | chr3.2:92845938-92845957 | MS.gene68764:intron | 40.0% | |
| ACGGAATAAACTGTGACACA+CGG | + | chr3.2:92846323-92846342 | None:intergenic | 40.0% | |
| AGTGAAAATTTGTGCTGGGA+AGG | + | chr3.2:92846152-92846171 | None:intergenic | 40.0% | |
| CAAATATGTTCGCAGTGAGT+TGG | - | chr3.2:92845936-92845955 | MS.gene68764:intron | 40.0% | |
| CAACTCACTGCGAACATATT+TGG | + | chr3.2:92845938-92845957 | None:intergenic | 40.0% | |
| CAATGACCTGGAAGTTTGAA+TGG | + | chr3.2:92846899-92846918 | None:intergenic | 40.0% | |
| CACTCATGTTCTTTCTGTGT+TGG | - | chr3.2:92846168-92846187 | MS.gene68764:intron | 40.0% | |
| CAGTTGGATTCATCAGTGAA+CGG | - | chr3.2:92846972-92846991 | MS.gene68764:CDS | 40.0% | |
| CATTAACCCACATAGCAACA+AGG | + | chr3.2:92846393-92846412 | None:intergenic | 40.0% | |
| CGGCAGTTCCAACATATATA+TGG | + | chr3.2:92846303-92846322 | None:intergenic | 40.0% | |
| GAATCCAAGGAGAGTCAAAA+TGG | - | chr3.2:92845422-92845441 | MS.gene68764:intron | 40.0% | |
| GGCAAATTGCAAGACGTTAT+CGG | + | chr3.2:92845490-92845509 | None:intergenic | 40.0% | |
| GTTAGTTCATCACTACGCAT+AGG | - | chr3.2:92846261-92846280 | MS.gene68764:intron | 40.0% | |
| GTTTCCCCACAATTATGAGA+TGG | - | chr3.2:92846355-92846374 | MS.gene68764:CDS | 40.0% | |
| TAAAGTGTATGGCGAATCCA+AGG | - | chr3.2:92845409-92845428 | MS.gene68764:intron | 40.0% | |
| TAAGGAAGCTTCAGGAAGAA+CGG | + | chr3.2:92845511-92845530 | None:intergenic | 40.0% | |
| TCTCATAATTGTGGGGAAAC+CGG | + | chr3.2:92846355-92846374 | None:intergenic | 40.0% | |
| TTTATTCCGTGATTTCCTGC+CGG | - | chr3.2:92846333-92846352 | MS.gene68764:CDS | 40.0% | |
| ! | AGATGAAGACGATGCTGATT+GGG | - | chr3.2:92847025-92847044 | MS.gene68764:CDS | 40.0% |
| ! | ATCCTCCAAAAAGCGTATCT+CGG | + | chr3.2:92845989-92846008 | None:intergenic | 40.0% |
| ! | CACTTTTAGTGTAGGTCGTT+AGG | - | chr3.2:92845845-92845864 | MS.gene68764:intron | 40.0% |
| ! | GATTTTCACTGCCTCTTGTA+TGG | - | chr3.2:92846461-92846480 | MS.gene68764:intron | 40.0% |
| ! | TTCTTGAAGCATTTCCTGTG+AGG | + | chr3.2:92845377-92845396 | None:intergenic | 40.0% |
| !! | AATCTTTTGAGAGAGGAAGC+AGG | - | chr3.2:92846010-92846029 | MS.gene68764:intron | 40.0% |
| !! | ATCTTTTGAGAGAGGAAGCA+GGG | - | chr3.2:92846011-92846030 | MS.gene68764:intron | 40.0% |
| !! | GAGGATGAATCTTTTGAGAG+AGG | - | chr3.2:92846003-92846022 | MS.gene68764:intron | 40.0% |
| !! | TGAAATCACCGCTGATTTAC+AGG | - | chr3.2:92845575-92845594 | MS.gene68764:intron | 40.0% |
| !! | TTTTGGAGATGAAAGAGGGA+AGG | - | chr3.2:92845882-92845901 | MS.gene68764:intron | 40.0% |
| !!! | TTTTGATTTCAACCCAGCCA+TGG | + | chr3.2:92845914-92845933 | None:intergenic | 40.0% |
| AGATGAAAGAGGGAAGGAAG+TGG | - | chr3.2:92845888-92845907 | MS.gene68764:intron | 45.0% | |
| ATATTTCAGACTCAGAGGCG+TGG | - | chr3.2:92845345-92845364 | MS.gene68764:CDS | 45.0% | |
| ATCAGCGGTGATTTCAACGT+CGG | + | chr3.2:92845571-92845590 | None:intergenic | 45.0% | |
| ATGACTGTCATGTAACGCTG+TGG | - | chr3.2:92845803-92845822 | MS.gene68764:intron | 45.0% | |
| CAAGAAACTGAGAGATGGTC+CGG | + | chr3.2:92846125-92846144 | None:intergenic | 45.0% | |
| CAGATGAAGACGATGCTGAT+TGG | - | chr3.2:92847024-92847043 | MS.gene68764:CDS | 45.0% | |
| CGGTTATTACATCGCCAACA+AGG | + | chr3.2:92845306-92845325 | None:intergenic | 45.0% | |
| GAAAATTTGTGCTGGGAAGG+AGG | + | chr3.2:92846149-92846168 | None:intergenic | 45.0% | |
| GAAGATGAAGACCCTGAAGA+AGG | - | chr3.2:92847047-92847066 | MS.gene68764:CDS | 45.0% | |
| GAGGTAAGAATCAGCACCTA+AGG | + | chr3.2:92845529-92845548 | None:intergenic | 45.0% | |
| GAGGTCAAGAAACTGAGAGA+TGG | + | chr3.2:92846130-92846149 | None:intergenic | 45.0% | |
| GGCGGCGAAAATGATAGAAT+AGG | + | chr3.2:92845615-92845634 | None:intergenic | 45.0% | |
| GTTCTTCCTGAAGCTTCCTT+AGG | - | chr3.2:92845510-92845529 | MS.gene68764:intron | 45.0% | |
| TTCCACCCATTCAAACTTCC+AGG | - | chr3.2:92846890-92846909 | MS.gene68764:CDS | 45.0% | |
| TTGTGGGTTTCACATCACTG+TGG | - | chr3.2:92846579-92846598 | MS.gene68764:intron | 45.0% | |
| ! | AACTCCCGAGATACGCTTTT+TGG | - | chr3.2:92845981-92846000 | MS.gene68764:intron | 45.0% |
| ! | GTGTTGTTCTGTTTCGGCTT+TGG | - | chr3.2:92846093-92846112 | MS.gene68764:intron | 45.0% |
| ! | TATTTCAGACTCAGAGGCGT+GGG | - | chr3.2:92845346-92845365 | MS.gene68764:CDS | 45.0% |
| ! | TCCTCCAAAAAGCGTATCTC+GGG | + | chr3.2:92845988-92846007 | None:intergenic | 45.0% |
| !! | AACAATGCTTTTGAGGGGAG+AGG | - | chr3.2:92846504-92846523 | MS.gene68764:intron | 45.0% |
| !! | ACAATGCTTTTGAGGGGAGA+GGG | - | chr3.2:92846505-92846524 | MS.gene68764:intron | 45.0% |
| !!! | GCAACCATTTTGACTCTCCT+TGG | + | chr3.2:92845429-92845448 | None:intergenic | 45.0% |
| AACTGAGAGATGGTCCGGAA+AGG | + | chr3.2:92846120-92846139 | None:intergenic | 50.0% | |
| ACTAGGCCCTTGTTGCTATG+TGG | - | chr3.2:92846383-92846402 | MS.gene68764:CDS | 50.0% | |
| ACTGAGAGATGGTCCGGAAA+GGG | + | chr3.2:92846119-92846138 | None:intergenic | 50.0% | |
| ATAATTGTGGGGAAACCGGC+AGG | + | chr3.2:92846351-92846370 | None:intergenic | 50.0% | |
| CTAGGCCCTTGTTGCTATGT+GGG | - | chr3.2:92846384-92846403 | MS.gene68764:CDS | 50.0% | |
| GACCTGGAAGTTTGAATGGG+TGG | + | chr3.2:92846895-92846914 | None:intergenic | 50.0% | |
| GGTGATTTCAACGTCGGTGA+TGG | + | chr3.2:92845565-92845584 | None:intergenic | 50.0% | |
| TAGTTGCAGTTGCGGTTGTG+CGG | + | chr3.2:92845326-92845345 | None:intergenic | 50.0% | |
| TCAGCACCTAAGGAAGCTTC+AGG | + | chr3.2:92845519-92845538 | None:intergenic | 50.0% | |
| TGGCATGCATAAGTGAGCAC+TGG | + | chr3.2:92846791-92846810 | None:intergenic | 50.0% | |
| ! | GATTTCAACGTCGGTGATGG+TGG | + | chr3.2:92845562-92845581 | None:intergenic | 50.0% |
| !! | GCATAAGTGAGCACTGGCTA+TGG | + | chr3.2:92846785-92846804 | None:intergenic | 50.0% |
| !! | TCCCGAGATACGCTTTTTGG+AGG | - | chr3.2:92845984-92846003 | MS.gene68764:intron | 50.0% |
| AAGAGGGAAGGAAGTGGCCA+TGG | - | chr3.2:92845894-92845913 | MS.gene68764:intron | 55.0% | |
| CAAGGTGTGAGTGTGACCGA+TGG | + | chr3.2:92845288-92845307 | None:intergenic | 55.0% | |
| CGGTCACACTCACACCTTGT+TGG | - | chr3.2:92845289-92845308 | MS.gene68764:CDS | 55.0% | |
| CTGTCATGTAACGCTGTGGC+TGG | - | chr3.2:92845807-92845826 | MS.gene68764:intron | 55.0% | |
| GACGCAATTGCTTCACACCG+CGG | - | chr3.2:92845251-92845270 | MS.gene68764:CDS | 55.0% | |
| GGGAAACCGGCAGGAAATCA+CGG | + | chr3.2:92846342-92846361 | None:intergenic | 55.0% | |
| ! | ATGTTCGCAGTGAGTTGGGG+AGG | - | chr3.2:92845941-92845960 | MS.gene68764:intron | 55.0% |
| ! | GTTTCGGCTTTGGCCCTTTC+CGG | - | chr3.2:92846103-92846122 | MS.gene68764:intron | 55.0% |
| ! | TGGGGAGGCGTATGAAGTTG+CGG | - | chr3.2:92845956-92845975 | MS.gene68764:intron | 55.0% |
| !! | AACGTCGGTGATGGTGGTGA+CGG | + | chr3.2:92845556-92845575 | None:intergenic | 55.0% |
| !! | ATGCTTTTGAGGGGAGAGGG+TGG | - | chr3.2:92846508-92846527 | MS.gene68764:intron | 55.0% |
| !! | TGATGGTGGTGACGGAAGAG+AGG | + | chr3.2:92845548-92845567 | None:intergenic | 55.0% |
| !! | TGCTTTTGAGGGGAGAGGGT+GGG | - | chr3.2:92846509-92846528 | MS.gene68764:intron | 55.0% |
| CGATGGTATCAGTGCAGCCG+CGG | + | chr3.2:92845271-92845290 | None:intergenic | 60.0% | |
| GGAAGGAAGTGGCCATGGCT+GGG | - | chr3.2:92845899-92845918 | MS.gene68764:intron | 60.0% | |
| ! | AGGGGAGAGGGTGGGTTCTT+TGG | - | chr3.2:92846517-92846536 | MS.gene68764:intron | 60.0% |
| ! | CGCGGCTGCACTGATACCAT+CGG | - | chr3.2:92845269-92845288 | MS.gene68764:CDS | 60.0% |
| ! | TTCAGACTCAGAGGCGTGGG+TGG | - | chr3.2:92845349-92845368 | MS.gene68764:CDS | 60.0% |
| AGGCGTGGGTGGATCCTCAC+AGG | - | chr3.2:92845360-92845379 | MS.gene68764:CDS | 65.0% | |
| GGGAAGGAAGTGGCCATGGC+TGG | - | chr3.2:92845898-92845917 | MS.gene68764:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 92845243 | 92847088 | 92845243 | ID=MS.gene68764 |
| chr3.2 | mRNA | 92845243 | 92847088 | 92845243 | ID=MS.gene68764.t1;Parent=MS.gene68764 |
| chr3.2 | exon | 92846735 | 92847088 | 92846735 | ID=MS.gene68764.t1.exon1;Parent=MS.gene68764.t1 |
| chr3.2 | CDS | 92846735 | 92847088 | 92846735 | ID=cds.MS.gene68764.t1;Parent=MS.gene68764.t1 |
| chr3.2 | exon | 92846299 | 92846472 | 92846299 | ID=MS.gene68764.t1.exon2;Parent=MS.gene68764.t1 |
| chr3.2 | CDS | 92846299 | 92846472 | 92846299 | ID=cds.MS.gene68764.t1;Parent=MS.gene68764.t1 |
| chr3.2 | exon | 92845243 | 92845419 | 92845243 | ID=MS.gene68764.t1.exon3;Parent=MS.gene68764.t1 |
| chr3.2 | CDS | 92845243 | 92845419 | 92845243 | ID=cds.MS.gene68764.t1;Parent=MS.gene68764.t1 |
| Gene Sequence |
| Protein sequence |
