AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene24247


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24247.t1 MTR_1g022450 100.000 76 0 0 1 76 61 136 4.57e-51 156
MS.gene24247.t1 MTR_1g022450 100.000 76 0 0 1 76 59 134 4.79e-51 156
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24247.t1 AT1G75350 84.127 63 10 0 1 63 71 133 6.09e-36 118

Find 24 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCAACCACATACTCTTTC+TGG 0.120517 1.2:+2041772 None:intergenic
ATGGAGATTCCTGTGCTTAA+AGG 0.247138 1.2:-2041642 MS.gene24247:CDS
CGGTGAATTGTCGCAGATTA+TGG 0.250630 1.2:-2041661 MS.gene24247:CDS
AGGTAACAGGTCTGCTCTTA+TGG 0.321423 1.2:-2041721 MS.gene24247:CDS
GACCTGTTACCTAGGTAAAA+TGG 0.333258 1.2:+2041732 None:intergenic
TTAATCCCTTTACGTCTAGA+AGG 0.369196 1.2:+2041606 None:intergenic
TTATTCCTTCTAGACGTAAA+GGG 0.415844 1.2:-2041611 MS.gene24247:CDS
ATCAACCACATACTCTTTCT+GGG 0.433365 1.2:+2041773 None:intergenic
GTTATTCCTTCTAGACGTAA+AGG 0.445368 1.2:-2041612 MS.gene24247:CDS
TATGTGGTTGATGTTTGGTC+TGG 0.461863 1.2:-2041762 MS.gene24247:CDS
TCTTATGGTGAGTGATGATC+AGG 0.475882 1.2:-2041706 MS.gene24247:CDS
AAGAGTATGTGGTTGATGTT+TGG 0.479372 1.2:-2041767 MS.gene24247:CDS
GAGAAGTTTCGTAAGAAGTT+CGG 0.485181 1.2:-2041681 MS.gene24247:CDS
AACGAGCTTGTGATGACAAC+TGG 0.499125 1.2:-2041801 None:intergenic
ATGTGGTTGATGTTTGGTCT+GGG 0.499973 1.2:-2041761 MS.gene24247:CDS
TAAGAGCAGACCTGTTACCT+AGG 0.535799 1.2:+2041724 None:intergenic
AAAGGGATTAAAAGTGGTGG+AGG 0.538533 1.2:-2041594 MS.gene24247:CDS
ACAATCTCACCTTTAAGCAC+AGG 0.575557 1.2:+2041633 None:intergenic
AGACGTAAAGGGATTAAAAG+TGG 0.579688 1.2:-2041600 MS.gene24247:CDS
CGTAAAGGGATTAAAAGTGG+TGG 0.606986 1.2:-2041597 MS.gene24247:CDS
AAGGGATTAAAAGTGGTGGA+GGG 0.644540 1.2:-2041593 MS.gene24247:CDS
TGTTACCTAGGTAAAATGGA+TGG 0.664203 1.2:+2041736 None:intergenic
TGGAACCCAGAAAGAGTATG+TGG 0.674951 1.2:-2041778 MS.gene24247:CDS
GAGCTTGTGATGACAACTGG+TGG 0.675964 1.2:-2041798 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TTATTCCTTCTAGACGTAAA+GGG - chr1.2:2041759-2041778 MS.gene24247:CDS 30.0%
AGACGTAAAGGGATTAAAAG+TGG - chr1.2:2041770-2041789 MS.gene24247:CDS 35.0%
ATCAACCACATACTCTTTCT+GGG + chr1.2:2041600-2041619 None:intergenic 35.0%
GAGAAGTTTCGTAAGAAGTT+CGG - chr1.2:2041689-2041708 MS.gene24247:CDS 35.0%
GTTATTCCTTCTAGACGTAA+AGG - chr1.2:2041758-2041777 MS.gene24247:CDS 35.0%
TGTTACCTAGGTAAAATGGA+TGG + chr1.2:2041637-2041656 None:intergenic 35.0%
TTAATCCCTTTACGTCTAGA+AGG + chr1.2:2041767-2041786 None:intergenic 35.0%
! AAGAGTATGTGGTTGATGTT+TGG - chr1.2:2041603-2041622 MS.gene24247:CDS 35.0%
! ATCCATTTTACCTAGGTAAC+AGG - chr1.2:2041636-2041655 MS.gene24247:CDS 35.0%
AAAGGGATTAAAAGTGGTGG+AGG - chr1.2:2041776-2041795 MS.gene24247:CDS 40.0%
ACAATCTCACCTTTAAGCAC+AGG + chr1.2:2041740-2041759 None:intergenic 40.0%
ATGGAGATTCCTGTGCTTAA+AGG - chr1.2:2041728-2041747 MS.gene24247:CDS 40.0%
CATCAACCACATACTCTTTC+TGG + chr1.2:2041601-2041620 None:intergenic 40.0%
CGTAAAGGGATTAAAAGTGG+TGG - chr1.2:2041773-2041792 MS.gene24247:CDS 40.0%
GACCTGTTACCTAGGTAAAA+TGG + chr1.2:2041641-2041660 None:intergenic 40.0%
TCTTATGGTGAGTGATGATC+AGG - chr1.2:2041664-2041683 MS.gene24247:CDS 40.0%
! AAGGGATTAAAAGTGGTGGA+GGG - chr1.2:2041777-2041796 MS.gene24247:CDS 40.0%
! ATGTGGTTGATGTTTGGTCT+GGG - chr1.2:2041609-2041628 MS.gene24247:CDS 40.0%
! TATGTGGTTGATGTTTGGTC+TGG - chr1.2:2041608-2041627 MS.gene24247:CDS 40.0%
AGGTAACAGGTCTGCTCTTA+TGG - chr1.2:2041649-2041668 MS.gene24247:CDS 45.0%
CGGTGAATTGTCGCAGATTA+TGG - chr1.2:2041709-2041728 MS.gene24247:CDS 45.0%
TAAGAGCAGACCTGTTACCT+AGG + chr1.2:2041649-2041668 None:intergenic 45.0%
! GGGAACCATCCATTTTACCT+AGG - chr1.2:2041629-2041648 MS.gene24247:CDS 45.0%
! TGGAACCCAGAAAGAGTATG+TGG - chr1.2:2041592-2041611 MS.gene24247:CDS 45.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 2041581 2041811 2041581 ID=MS.gene24247
chr1.2 mRNA 2041581 2041811 2041581 ID=MS.gene24247.t1;Parent=MS.gene24247
chr1.2 exon 2041581 2041811 2041581 ID=MS.gene24247.t1.exon1;Parent=MS.gene24247.t1
chr1.2 CDS 2041581 2041811 2041581 ID=cds.MS.gene24247.t1;Parent=MS.gene24247.t1
Gene Sequence

>MS.gene24247

ATGACAACTGGTGGAACCCAGAAAGAGTATGTGGTTGATGTTTGGTCTGGGAACCATCCATTTTACCTAGGTAACAGGTCTGCTCTTATGGTGAGTGATGATCAGGTAGAGAAGTTTCGTAAGAAGTTCGGTGAATTGTCGCAGATTATGGAGATTCCTGTGCTTAAAGGTGAGATTGTTATTCCTTCTAGACGTAAAGGGATTAAAAGTGGTGGAGGGAAGAAGAAGTGA

Protein sequence

>MS.gene24247.t1

MTTGGTQKEYVVDVWSGNHPFYLGNRSALMVSDDQVEKFRKKFGELSQIMEIPVLKGEIVIPSRRKGIKSGGGKKK