AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene46176


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene46176.t1 MTR_3g465210 87.179 156 20 0 7 162 131 286 1.51e-95 280
MS.gene46176.t1 MTR_2g074890 56.069 173 71 2 6 173 303 475 9.29e-54 178
MS.gene46176.t1 MTR_4g020650 43.165 139 44 3 3 141 107 210 6.88e-25 98.2
MS.gene46176.t1 MTR_1g093780 38.298 188 85 7 25 200 341 509 5.59e-24 99.0
MS.gene46176.t1 MTR_6g004090 39.844 128 47 4 2 128 145 243 1.10e-20 88.2
MS.gene46176.t1 MTR_3g062250 37.222 180 81 6 19 186 407 566 2.00e-19 85.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 49 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGGCAGCATACAAATTCTT+AGG 0.239081 7.2:-40118056 None:intergenic
GACAATACTGTTACCATCTA+AGG 0.261816 7.2:-40118336 None:intergenic
CCTGCGTTGGACCCTGTTAA+TGG 0.262725 7.2:+40117819 MS.gene46176:CDS
GTAAAATCTTGAGTAAGTTT+TGG 0.291642 7.2:+40118090 MS.gene46176:CDS
AAAGTCTGCCTCCCATTAAC+AGG 0.322459 7.2:-40117831 None:intergenic
TTCTTCCTGCCTTTCCTGCC+TGG 0.324269 7.2:-40118302 None:intergenic
CACTTCATGCTTGGTCCTTA+AGG 0.334146 7.2:-40118394 None:intergenic
AGAGAAAAGGGACTTGTAAT+GGG 0.372414 7.2:-40117855 None:intergenic
TGTGCCCCATGGTCAGTATC+AGG 0.377851 7.2:-40118152 None:intergenic
AAAGCTGCTAAAACCTTAGA+TGG 0.379329 7.2:+40118323 MS.gene46176:CDS
CTGCAACATTCTCATGAAGA+TGG 0.393420 7.2:+40118269 MS.gene46176:CDS
GAAGACTAGTGTTCGCCTTA+AGG 0.406300 7.2:+40118379 MS.gene46176:CDS
TAGAGAAAAGGGACTTGTAA+TGG 0.430477 7.2:-40117856 None:intergenic
CTGCGTTGGACCCTGTTAAT+GGG 0.438681 7.2:+40117820 MS.gene46176:CDS
CAATAGATGCACTTCATGCT+TGG 0.444927 7.2:-40118403 None:intergenic
AGTAGTGATGAGAGAAATGA+TGG 0.455410 7.2:-40117982 None:intergenic
AGTGACACAACAGTAGCATT+GGG 0.466348 7.2:-40117912 None:intergenic
AGAGTGAGTTTCCTTCAGAT+TGG 0.475190 7.2:-40118231 None:intergenic
GCTACTCTGATCTAGAGAAA+AGG 0.476642 7.2:-40117868 None:intergenic
TTTAGGGCAAGACAAGAGAA+AGG 0.484388 7.2:+40118016 MS.gene46176:CDS
TTAAACCAGGCAGGAAAGGC+AGG 0.487147 7.2:+40118297 MS.gene46176:CDS
CTACTCTGATCTAGAGAAAA+GGG 0.490610 7.2:-40117867 None:intergenic
GAGAATGTTGCAGAAGGTAC+TGG 0.497175 7.2:-40118259 None:intergenic
TAGATCAGAGTAGCAACATT+GGG 0.512574 7.2:+40117877 MS.gene46176:CDS
GAGCAACTGTCAAAGCTCAC+TGG 0.513373 7.2:+40118178 MS.gene46176:CDS
CTAGATCAGAGTAGCAACAT+TGG 0.525289 7.2:+40117876 MS.gene46176:CDS
TATGCTGCCTGTTTGAAAGA+TGG 0.526527 7.2:+40118068 MS.gene46176:CDS
AGGCAGCATACAAATTCTTA+GGG 0.529642 7.2:-40118055 None:intergenic
TTTATTAAACCAGGCAGGAA+AGG 0.534943 7.2:+40118293 MS.gene46176:CDS
AAGTGACACAACAGTAGCAT+TGG 0.538667 7.2:-40117913 None:intergenic
TTGTGTCACTTAGTGACGCT+AGG 0.559850 7.2:+40117925 MS.gene46176:CDS
AGAAAGGTTCACTTCACTGC+TGG 0.569576 7.2:+40118032 MS.gene46176:CDS
GAGTTCAGGTAATCCTGCGT+TGG 0.581585 7.2:+40117806 MS.gene46176:intron
TGGAACCTGATACTGACCAT+GGG 0.590117 7.2:+40118147 MS.gene46176:CDS
TTGGAACCTGATACTGACCA+TGG 0.590328 7.2:+40118146 MS.gene46176:CDS
TGTGTCACTTAGTGACGCTA+GGG 0.600168 7.2:+40117926 MS.gene46176:CDS
TGGTACTCTAGCCAATCTGA+AGG 0.601845 7.2:+40118220 MS.gene46176:CDS
AGTTGCTCTTGTGTGCCCCA+TGG 0.612741 7.2:-40118163 None:intergenic
CTTCATGAGAATGTTGCAGA+AGG 0.612840 7.2:-40118265 None:intergenic
CCATTAACAGGGTCCAACGC+AGG 0.629983 7.2:-40117819 None:intergenic
GAGAAAAGGGACTTGTAATG+GGG 0.631154 7.2:-40117854 None:intergenic
TGATGCTACTAATAGCACAT+TGG 0.638027 7.2:+40118127 MS.gene46176:CDS
CGTTGGACCCTGTTAATGGG+AGG 0.639115 7.2:+40117823 MS.gene46176:CDS
ATCAGAGTAGCAACATTGGG+AGG 0.647982 7.2:+40117880 MS.gene46176:CDS
GAGCATGATATAGACACAGA+TGG 0.650912 7.2:+40118200 MS.gene46176:CDS
AAGTCTGCCTCCCATTAACA+GGG 0.677418 7.2:-40117830 None:intergenic
GTGACACAACAGTAGCATTG+GGG 0.683045 7.2:-40117911 None:intergenic
GGAACCTGATACTGACCATG+GGG 0.712683 7.2:+40118148 MS.gene46176:CDS
GTAGTGATGAGAGAAATGAT+GGG 0.786253 7.2:-40117981 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATAAAATGTAATCTC+CGG - chr7.2:40117548-40117567 None:intergenic 15.0%
!!! TTTAGCATTATTTTTTGTAA+TGG - chr7.2:40117225-40117244 None:intergenic 15.0%
!!! TTTAGTATTATTTTTTGTTG+AGG + chr7.2:40117341-40117360 MS.gene46176:intron 15.0%
!! ACATATATGATATATCCAAA+AGG - chr7.2:40117661-40117680 None:intergenic 20.0%
!! ATTCCTTCTTTATAATATGT+CGG - chr7.2:40117287-40117306 None:intergenic 20.0%
!! GGATATATCATATATGTAAT+TGG + chr7.2:40117664-40117683 MS.gene46176:intron 20.0%
!! GTTATGAAATTTATATATCG+AGG + chr7.2:40117249-40117268 MS.gene46176:intron 20.0%
!!! TTTATTGTTGTTTCTGATTA+TGG + chr7.2:40116998-40117017 MS.gene46176:intron 20.0%
! GTCACATTATCATGAAAAAT+AGG - chr7.2:40117954-40117973 None:intergenic 25.0%
! TTCCTTCTTTATAATATGTC+GGG - chr7.2:40117286-40117305 None:intergenic 25.0%
! TTGTTGAGGATTAAATTGAA+GGG + chr7.2:40117355-40117374 MS.gene46176:intron 25.0%
! TTTGTTGAGGATTAAATTGA+AGG + chr7.2:40117354-40117373 MS.gene46176:intron 25.0%
!! ACTACTAATGATGAGATTTT+AGG + chr7.2:40117999-40118018 MS.gene46176:CDS 25.0%
!! ATGATCTAAGATAAGGTAAA+GGG - chr7.2:40117107-40117126 None:intergenic 25.0%
!! CTACTAATGATGAGATTTTA+GGG + chr7.2:40118000-40118019 MS.gene46176:CDS 25.0%
!! GTAAAATCTTGAGTAAGTTT+TGG + chr7.2:40118090-40118109 MS.gene46176:CDS 25.0%
!! TTTTAAACTCAATGCTCATT+TGG + chr7.2:40117167-40117186 MS.gene46176:intron 25.0%
!! TTTTTATGTCAAATTGCATC+TGG + chr7.2:40117418-40117437 MS.gene46176:intron 25.0%
AATCGTCCCTTTATTAAATG+TGG + chr7.2:40117689-40117708 MS.gene46176:intron 30.0%
ATCCTGCTAAATTGGATAAA+AGG + chr7.2:40117763-40117782 MS.gene46176:intron 30.0%
ATCGTCCCTTTATTAAATGT+GGG + chr7.2:40117690-40117709 MS.gene46176:intron 30.0%
GTTAGTGTAGCACTAAAATA+GGG + chr7.2:40117035-40117054 MS.gene46176:intron 30.0%
TATAGATGATCCTGCTAAAT+TGG + chr7.2:40117755-40117774 MS.gene46176:intron 30.0%
TGAAGGGTAATAAAATTGTC+AGG + chr7.2:40117371-40117390 MS.gene46176:intron 30.0%
TGCAAGATACGTATATGATA+CGG - chr7.2:40117482-40117501 None:intergenic 30.0%
TGTTAGTGTAGCACTAAAAT+AGG + chr7.2:40117034-40117053 MS.gene46176:intron 30.0%
TTCCCGACATATTATAAAGA+AGG + chr7.2:40117281-40117300 MS.gene46176:intron 30.0%
! AATACTTTTCAGATACATCG+AGG + chr7.2:40117584-40117603 MS.gene46176:intron 30.0%
! ATACTTTTCAGATACATCGA+GGG + chr7.2:40117585-40117604 MS.gene46176:intron 30.0%
! GATGATCTAAGATAAGGTAA+AGG - chr7.2:40117108-40117127 None:intergenic 30.0%
! GATTTTACCATCTTTCAAAC+AGG - chr7.2:40118078-40118097 None:intergenic 30.0%
! TCCATAACATTTTCAAAACG+AGG - chr7.2:40117317-40117336 None:intergenic 30.0%
! TTCCTTTTATCCAATTTAGC+AGG - chr7.2:40117768-40117787 None:intergenic 30.0%
!! AAATCTTGAGTAAGTTTTGG+AGG + chr7.2:40118093-40118112 MS.gene46176:CDS 30.0%
!!! AATCTTGAGTAAGTTTTGGA+GGG + chr7.2:40118094-40118113 MS.gene46176:CDS 30.0%
!!! GAAGATGGTTTTATTAAACC+AGG + chr7.2:40118284-40118303 MS.gene46176:CDS 30.0%
!!! TCCTCGTTTTGAAAATGTTA+TGG + chr7.2:40117313-40117332 MS.gene46176:intron 30.0%
AAAGCTGCTAAAACCTTAGA+TGG + chr7.2:40118323-40118342 MS.gene46176:CDS 35.0%
AGAGAAAAGGGACTTGTAAT+GGG - chr7.2:40117858-40117877 None:intergenic 35.0%
AGGCAGCATACAAATTCTTA+GGG - chr7.2:40118058-40118077 None:intergenic 35.0%
AGTAGTGATGAGAGAAATGA+TGG - chr7.2:40117985-40118004 None:intergenic 35.0%
CTACTCTGATCTAGAGAAAA+GGG - chr7.2:40117870-40117889 None:intergenic 35.0%
GACAATACTGTTACCATCTA+AGG - chr7.2:40118339-40118358 None:intergenic 35.0%
GTAGTGATGAGAGAAATGAT+GGG - chr7.2:40117984-40118003 None:intergenic 35.0%
TAGAGAAAAGGGACTTGTAA+TGG - chr7.2:40117859-40117878 None:intergenic 35.0%
TAGATCAGAGTAGCAACATT+GGG + chr7.2:40117877-40117896 MS.gene46176:CDS 35.0%
TCGTCCCTTTATTAAATGTG+GGG + chr7.2:40117691-40117710 MS.gene46176:intron 35.0%
TGATGCTACTAATAGCACAT+TGG + chr7.2:40118127-40118146 MS.gene46176:CDS 35.0%
TTCCCCCCACATTTAATAAA+GGG - chr7.2:40117698-40117717 None:intergenic 35.0%
TTTATTAAACCAGGCAGGAA+AGG + chr7.2:40118293-40118312 MS.gene46176:CDS 35.0%
! ACGTGTGATGATCTAAGATA+AGG - chr7.2:40117114-40117133 None:intergenic 35.0%
! GATTAACAGTGTTCTCCTTT+TGG + chr7.2:40117643-40117662 MS.gene46176:intron 35.0%
!!! ATGGTTTTATTAAACCAGGC+AGG + chr7.2:40118288-40118307 MS.gene46176:CDS 35.0%
AAGTGACACAACAGTAGCAT+TGG - chr7.2:40117916-40117935 None:intergenic 40.0%
AGAGTGAGTTTCCTTCAGAT+TGG - chr7.2:40118234-40118253 None:intergenic 40.0%
AGTGACACAACAGTAGCATT+GGG - chr7.2:40117915-40117934 None:intergenic 40.0%
CAGGCAGCATACAAATTCTT+AGG - chr7.2:40118059-40118078 None:intergenic 40.0%
CGTCCCTTTATTAAATGTGG+GGG + chr7.2:40117692-40117711 MS.gene46176:intron 40.0%
CTAGATCAGAGTAGCAACAT+TGG + chr7.2:40117876-40117895 MS.gene46176:CDS 40.0%
CTGCAACATTCTCATGAAGA+TGG + chr7.2:40118269-40118288 MS.gene46176:CDS 40.0%
CTTCATGAGAATGTTGCAGA+AGG - chr7.2:40118268-40118287 None:intergenic 40.0%
CTTCCCCCCACATTTAATAA+AGG - chr7.2:40117699-40117718 None:intergenic 40.0%
GAGAAAAGGGACTTGTAATG+GGG - chr7.2:40117857-40117876 None:intergenic 40.0%
GAGCATGATATAGACACAGA+TGG + chr7.2:40118200-40118219 MS.gene46176:CDS 40.0%
GCTACTCTGATCTAGAGAAA+AGG - chr7.2:40117871-40117890 None:intergenic 40.0%
GTCCCTTTATTAAATGTGGG+GGG + chr7.2:40117693-40117712 MS.gene46176:intron 40.0%
TAGTGCTACACTAACATCAG+AGG - chr7.2:40117030-40117049 None:intergenic 40.0%
TATGCTGCCTGTTTGAAAGA+TGG + chr7.2:40118068-40118087 MS.gene46176:CDS 40.0%
! AGTCAAGTTTTCCCTCTCTA+AGG - chr7.2:40117724-40117743 None:intergenic 40.0%
! TTTAGGGCAAGACAAGAGAA+AGG + chr7.2:40118016-40118035 MS.gene46176:CDS 40.0%
AAAGTCTGCCTCCCATTAAC+AGG - chr7.2:40117834-40117853 None:intergenic 45.0%
AAGTCTGCCTCCCATTAACA+GGG - chr7.2:40117833-40117852 None:intergenic 45.0%
AGAAAGGTTCACTTCACTGC+TGG + chr7.2:40118032-40118051 MS.gene46176:CDS 45.0%
ATACTCTCCCGATACGTATC+GGG - chr7.2:40117525-40117544 None:intergenic 45.0%
ATCAGAGTAGCAACATTGGG+AGG + chr7.2:40117880-40117899 MS.gene46176:CDS 45.0%
GAAGACTAGTGTTCGCCTTA+AGG + chr7.2:40118379-40118398 MS.gene46176:CDS 45.0%
GATACTCTCCCGATACGTAT+CGG - chr7.2:40117526-40117545 None:intergenic 45.0%
GGGGTGTATCAGACAAGTAT+AGG - chr7.2:40117505-40117524 None:intergenic 45.0%
GTGACACAACAGTAGCATTG+GGG - chr7.2:40117914-40117933 None:intergenic 45.0%
TGATACACCCCGATACGTAT+CGG + chr7.2:40117514-40117533 MS.gene46176:intron 45.0%
TGGAACCTGATACTGACCAT+GGG + chr7.2:40118147-40118166 MS.gene46176:CDS 45.0%
TGGTACTCTAGCCAATCTGA+AGG + chr7.2:40118220-40118239 MS.gene46176:CDS 45.0%
TTGGAACCTGATACTGACCA+TGG + chr7.2:40118146-40118165 MS.gene46176:CDS 45.0%
! GAGAATGTTGCAGAAGGTAC+TGG - chr7.2:40118262-40118281 None:intergenic 45.0%
!! ACACTTGCTGCTTTGAGTTC+AGG + chr7.2:40117792-40117811 MS.gene46176:intron 45.0%
!! ATACGTATCGGGAGAGTATC+CGG + chr7.2:40117526-40117545 MS.gene46176:intron 45.0%
!! TGTGTCACTTAGTGACGCTA+GGG + chr7.2:40117926-40117945 MS.gene46176:CDS 45.0%
!! TTGTGTCACTTAGTGACGCT+AGG + chr7.2:40117925-40117944 MS.gene46176:CDS 45.0%
!!! GAGTAAGTTTTGGAGGGATG+AGG + chr7.2:40118100-40118119 MS.gene46176:CDS 45.0%
CTGCGTTGGACCCTGTTAAT+GGG + chr7.2:40117820-40117839 MS.gene46176:CDS 50.0%
GAGCAACTGTCAAAGCTCAC+TGG + chr7.2:40118178-40118197 MS.gene46176:CDS 50.0%
GAGTTCAGGTAATCCTGCGT+TGG + chr7.2:40117806-40117825 MS.gene46176:intron 50.0%
GATACACCCCGATACGTATC+GGG + chr7.2:40117515-40117534 MS.gene46176:intron 50.0%
GGAACCTGATACTGACCATG+GGG + chr7.2:40118148-40118167 MS.gene46176:CDS 50.0%
TACTCTCCCGATACGTATCG+GGG - chr7.2:40117524-40117543 None:intergenic 50.0%
TTAAACCAGGCAGGAAAGGC+AGG + chr7.2:40118297-40118316 MS.gene46176:CDS 50.0%
AGTTGCTCTTGTGTGCCCCA+TGG - chr7.2:40118166-40118185 None:intergenic 55.0%
CCATTAACAGGGTCCAACGC+AGG - chr7.2:40117822-40117841 None:intergenic 55.0%
CCTGCGTTGGACCCTGTTAA+TGG + chr7.2:40117819-40117838 MS.gene46176:CDS 55.0%
CGTTGGACCCTGTTAATGGG+AGG + chr7.2:40117823-40117842 MS.gene46176:CDS 55.0%
GGGGGGAAGTTCCTTAGAGA+GGG + chr7.2:40117710-40117729 MS.gene46176:intron 55.0%
TGGGGGGAAGTTCCTTAGAG+AGG + chr7.2:40117709-40117728 MS.gene46176:intron 55.0%
TGTGCCCCATGGTCAGTATC+AGG - chr7.2:40118155-40118174 None:intergenic 55.0%
TTCTTCCTGCCTTTCCTGCC+TGG - chr7.2:40118305-40118324 None:intergenic 55.0%
GGTACGCGAGCGATTGACGA+TGG + chr7.2:40117606-40117625 MS.gene46176:intron 60.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 40116958 40118412 40116958 ID=MS.gene46176
chr7.2 mRNA 40116958 40118412 40116958 ID=MS.gene46176.t1;Parent=MS.gene46176
chr7.2 exon 40116958 40116976 40116958 ID=MS.gene46176.t1.exon1;Parent=MS.gene46176.t1
chr7.2 CDS 40116958 40116976 40116958 ID=cds.MS.gene46176.t1;Parent=MS.gene46176.t1
chr7.2 exon 40117814 40118412 40117814 ID=MS.gene46176.t1.exon2;Parent=MS.gene46176.t1
chr7.2 CDS 40117814 40118412 40117814 ID=cds.MS.gene46176.t1;Parent=MS.gene46176.t1
Gene Sequence

>MS.gene46176

ATGCAAGATTTTAGTTCAAGTATATTATAAATAGCATGTTTTTATTGTTGTTTCTGATTATGGTTGTTGCCTCTGATGTTAGTGTAGCACTAAAATAGGGTTTTTGTTGTTCTTTTTGTTTACTTTCACATTTCAGATTTTCTTATCCCTTTACCTTATCTTAGATCATCACACGTTATGTGACAAATGTAACTTGCTGCTATTTTTTTTTTTAAACTCAATGCTCATTTGGTTTTTTACAATTTTCTTGTATGTCAATTTAGTCCATTACAAAAAATAATGCTAAAATTAGTTATGAAATTTATATATCGAGGATAAATTAGTTCCCGACATATTATAAAGAAGGAATAATTTGTCCTCGTTTTGAAAATGTTATGGATTAATTTAGTATTATTTTTTGTTGAGGATTAAATTGAAGGGTAATAAAATTGTCAGGAAGCAAATTAAATTTTCGCTCTTTTTTTTATGTCAAATTGCATCTGGAGATTCTGAAGCTTAGATACTTCAAAATAGACGACGTACCGTATCATATACGTATCTTGCACCTATACTTGTCTGATACACCCCGATACGTATCGGGAGAGTATCCGGAGATTACATTTTATTTTTTTAAAATACAATTGTTCAATACTTTTCAGATACATCGAGGGTACGCGAGCGATTGACGATGGAAAGCGATACACGCGATTAACAGTGTTCTCCTTTTGGATATATCATATATGTAATTGGCTAATCGTCCCTTTATTAAATGTGGGGGGAAGTTCCTTAGAGAGGGAAAACTTGACTCATAGCTCAATTATAGATGATCCTGCTAAATTGGATAAAAGGAACTTTACACTTGCTGCTTTGAGTTCAGGTAATCCTGCGTTGGACCCTGTTAATGGGAGGCAGACTTTCCCCATTACAAGTCCCTTTTCTCTAGATCAGAGTAGCAACATTGGGAGGTTGTTATTCCCCAATGCTACTGTTGTGTCACTTAGTGACGCTAGGGATCCTATTTTTCATGATAATGTGACACTGCAGCCCATCATTTCTCTCATCACTACTAATGATGAGATTTTAGGGCAAGACAAGAGAAAGGTTCACTTCACTGCTGGCCCTAAGAATTTGTATGCTGCCTGTTTGAAAGATGGTAAAATCTTGAGTAAGTTTTGGAGGGATGAGGACACTGATGCTACTAATAGCACATTGGAACCTGATACTGACCATGGGGCACACAAGAGCAACTGTCAAAGCTCACTGGAGCATGATATAGACACAGATGGTACTCTAGCCAATCTGAAGGAAACTCACTCTGCTAACCAGTACCTTCTGCAACATTCTCATGAAGATGGTTTTATTAAACCAGGCAGGAAAGGCAGGAAGAAAGCTGCTAAAACCTTAGATGGTAACAGTATTGTCAGAGTTTCAATTAGACATAAGAAGACTAGTGTTCGCCTTAAGGACCAAGCATGA

Protein sequence

>MS.gene46176.t1

MQDFSSSNPALDPVNGRQTFPITSPFSLDQSSNIGRLLFPNATVVSLSDARDPIFHDNVTLQPIISLITTNDEILGQDKRKVHFTAGPKNLYAACLKDGKILSKFWRDEDTDATNSTLEPDTDHGAHKSNCQSSLEHDIDTDGTLANLKETHSANQYLLQHSHEDGFIKPGRKGRKKAAKTLDGNSIVRVSIRHKKTSVRLKDQA