AlfalfaGEDB | MS.gene057785

M. sativa cultivar XinJiangDaYe / MS.gene057785

KEGG: K14488
GO: GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
PFAM: PF02519.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057785.t1	PNY03825.1	83.3	78	13	0	1	78	1	78	6.30E-31	142.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057785.t1	P33080	68.8	77	24	0	1	77	1	77	1.4e-26	119.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057785.t1	A0A2K3NLB0	83.3	78	13	0	1	78	1	78	4.5e-31	142.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene003627	MS.gene057785	PPI
MS.gene003577	MS.gene057785	PPI
MS.gene057785	MS.gene75672	PPI
MS.gene77524	MS.gene057785	PPI
MS.gene062984	MS.gene057785	PPI
MS.gene057801	MS.gene057785	PPI
MS.gene057785	MS.gene062974	PPI
MS.gene014222	MS.gene057785	PPI
MS.gene40327	MS.gene057785	PPI
MS.gene031201	MS.gene057785	PPI
MS.gene057785	MS.gene014670	PPI
MS.gene045362	MS.gene057785	PPI
MS.gene77523	MS.gene057785	PPI
MS.gene003587	MS.gene057785	PPI
MS.gene003590	MS.gene057785	PPI
MS.gene045368	MS.gene057785	PPI
MS.gene063112	MS.gene057785	PPI
MS.gene79891	MS.gene057785	PPI
MS.gene057785	MS.gene50684	PPI
MS.gene79887	MS.gene057785	PPI
MS.gene062973	MS.gene057785	PPI
MS.gene057785	MS.gene79886	PPI
MS.gene77526	MS.gene057785	PPI
MS.gene047517	MS.gene057785	PPI
MS.gene057785	MS.gene50765	PPI
MS.gene045360	MS.gene057785	PPI
MS.gene014669	MS.gene057785	PPI
MS.gene057818	MS.gene057785	PPI
MS.gene68162	MS.gene057785	PPI
MS.gene014223	MS.gene057785	PPI
MS.gene50687	MS.gene057785	PPI
MS.gene75677	MS.gene057785	PPI
MS.gene057785	MS.gene045367	PPI
MS.gene057795	MS.gene057785	PPI
MS.gene68165	MS.gene057785	PPI
MS.gene01764	MS.gene057785	PPI
MS.gene045361	MS.gene057785	PPI
MS.gene50675	MS.gene057785	PPI
MS.gene003591	MS.gene057785	PPI
MS.gene057785	MS.gene057808	PPI
MS.gene057785	MS.gene002380	PPI
MS.gene062979	MS.gene057785	PPI
MS.gene40325	MS.gene057785	PPI
MS.gene062943	MS.gene057785	PPI
MS.gene01358	MS.gene057785	PPI
MS.gene014668	MS.gene057785	PPI
MS.gene003608	MS.gene057785	PPI
MS.gene75675	MS.gene057785	PPI
MS.gene003583	MS.gene057785	PPI
MS.gene79888	MS.gene057785	PPI
MS.gene057785	MS.gene68163	PPI
MS.gene40326	MS.gene057785	PPI
MS.gene062952	MS.gene057785	PPI
MS.gene057785	MS.gene75315	PPI
MS.gene77527	MS.gene057785	PPI
MS.gene50654	MS.gene057785	PPI
MS.gene50676	MS.gene057785	PPI
MS.gene003614	MS.gene057785	PPI
MS.gene057797	MS.gene057785	PPI
MS.gene50652	MS.gene057785	PPI
MS.gene23370	MS.gene057785	PPI
MS.gene50766	MS.gene057785	PPI
MS.gene003594	MS.gene057785	PPI
MS.gene003596	MS.gene057785	PPI
MS.gene50665	MS.gene057785	PPI
MS.gene79889	MS.gene057785	PPI
MS.gene057785	MS.gene75678	PPI
MS.gene50677	MS.gene057785	PPI
MS.gene003625	MS.gene057785	PPI
MS.gene68759	MS.gene057785	PPI
MS.gene003586	MS.gene057785	PPI
MS.gene062988	MS.gene057785	PPI
MS.gene77528	MS.gene057785	PPI
MS.gene50763	MS.gene057785	PPI
MS.gene031200	MS.gene057785	PPI
MS.gene045365	MS.gene057785	PPI
MS.gene79885	MS.gene057785	PPI
MS.gene50659	MS.gene057785	PPI
MS.gene75316	MS.gene057785	PPI
MS.gene057790	MS.gene057785	PPI
MS.gene045363	MS.gene057785	PPI
MS.gene003624	MS.gene057785	PPI
MS.gene23371	MS.gene057785	PPI
MS.gene031199	MS.gene057785	PPI
MS.gene75314	MS.gene057785	PPI
MS.gene77529	MS.gene057785	PPI
MS.gene68761	MS.gene057785	PPI
MS.gene014667	MS.gene057785	PPI
MS.gene68760	MS.gene057785	PPI
MS.gene75676	MS.gene057785	PPI
MS.gene062951	MS.gene057785	PPI
MS.gene057785	MS.gene75679	PPI
MS.gene057778	MS.gene057785	PPI
MS.gene75673	MS.gene057785	PPI
MS.gene045366	MS.gene057785	PPI
MS.gene062963	MS.gene057785	PPI
MS.gene68164	MS.gene057785	PPI
MS.gene031198	MS.gene057785	PPI
MS.gene75674	MS.gene057785	PPI
MS.gene79890	MS.gene057785	PPI
MS.gene003615	MS.gene057785	PPI
MS.gene057785	MS.gene50764	PPI
MS.gene045364	MS.gene057785	PPI
MS.gene003602	MS.gene057785	PPI
MS.gene23372	MS.gene057785	PPI
MS.gene057785	MS.gene014671	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057785.t1	MTR_4g072680	80.769	78	15	0	1	78	1	78	8.74e-45	139
MS.gene057785.t1	MTR_4g072620	79.487	78	16	0	1	78	1	78	1.12e-44	139
MS.gene057785.t1	MTR_4g072460	79.487	78	16	0	1	78	1	78	5.29e-44	137
MS.gene057785.t1	MTR_4g072480	78.205	78	17	0	1	78	1	78	1.07e-43	136
MS.gene057785.t1	MTR_4g072730	75.641	78	19	0	1	78	1	78	1.71e-42	133
MS.gene057785.t1	MTR_4g072570	79.487	78	16	0	1	78	1	78	8.51e-42	131
MS.gene057785.t1	MTR_4g072530	74.359	78	20	0	1	78	1	78	9.30e-39	124
MS.gene057785.t1	MTR_4g072500	73.077	78	21	0	1	78	1	78	2.80e-38	122
MS.gene057785.t1	MTR_4g072660	71.795	78	17	1	1	78	1	73	4.14e-38	122
MS.gene057785.t1	MTR_4g072310	72.152	79	21	1	1	78	1	79	1.16e-37	121
MS.gene057785.t1	MTR_3g084150	69.231	78	24	0	1	78	1	78	1.39e-36	118
MS.gene057785.t1	MTR_3g084180	69.231	78	24	0	1	78	1	78	1.97e-36	118
MS.gene057785.t1	MTR_4g072640	71.795	78	22	0	1	78	1	78	2.30e-36	117
MS.gene057785.t1	MTR_8g461410	66.667	78	26	0	1	78	1	78	3.35e-35	115
MS.gene057785.t1	MTR_4g072870	65.823	79	26	1	1	78	1	79	3.40e-35	115
MS.gene057785.t1	MTR_3g110042	67.949	78	25	0	1	78	1	78	1.38e-34	113
MS.gene057785.t1	MTR_4g072230	69.620	79	21	2	1	78	1	77	1.88e-34	113
MS.gene057785.t1	MTR_4g072270	67.089	79	25	1	1	78	1	79	3.33e-34	112
MS.gene057785.t1	MTR_4g072880	67.089	79	25	1	1	78	1	79	3.68e-34	112
MS.gene057785.t1	MTR_3g117630	72.222	72	20	0	7	78	7	78	4.21e-34	112
MS.gene057785.t1	MTR_3g084240	65.385	78	27	0	1	78	1	78	5.99e-34	111
MS.gene057785.t1	MTR_4g072240	62.025	79	29	1	1	78	1	79	6.01e-34	111
MS.gene057785.t1	MTR_4g072370	68.831	77	22	1	1	77	1	75	7.43e-34	111
MS.gene057785.t1	MTR_3g084260	68.354	79	24	1	1	78	1	79	9.14e-34	111
MS.gene057785.t1	MTR_4g072250	69.620	79	22	2	1	78	1	78	1.32e-33	110
MS.gene057785.t1	MTR_3g084170	65.385	78	27	0	1	78	1	78	2.14e-33	110
MS.gene057785.t1	MTR_4g072300	60.759	79	30	1	1	78	1	79	2.34e-33	110
MS.gene057785.t1	MTR_5g008360	78.788	66	14	0	13	78	4	69	2.48e-33	110
MS.gene057785.t1	MTR_4g072220	62.025	79	29	1	1	78	1	79	2.74e-33	110
MS.gene057785.t1	MTR_4g072860	67.949	78	24	1	1	77	1	78	4.20e-33	109
MS.gene057785.t1	MTR_4g072890	62.821	78	28	1	1	77	1	78	6.93e-33	108
MS.gene057785.t1	MTR_4g072260	62.821	78	28	1	1	77	1	78	6.95e-33	109
MS.gene057785.t1	MTR_3g110032	66.667	78	25	1	1	77	1	78	9.56e-33	108
MS.gene057785.t1	MTR_4g072580	67.532	77	20	1	1	77	1	72	1.01e-32	108
MS.gene057785.t1	MTR_3g084220	62.821	78	29	0	1	78	1	78	2.16e-32	107
MS.gene057785.t1	MTR_3g110038	62.821	78	29	0	1	78	1	78	2.94e-32	107
MS.gene057785.t1	MTR_3g084250	63.636	77	28	0	1	77	1	77	5.49e-32	107
MS.gene057785.t1	MTR_4g050700	65.823	79	26	1	1	78	1	79	5.90e-32	106
MS.gene057785.t1	MTR_2g012410	65.823	79	26	1	1	78	1	79	5.90e-32	106
MS.gene057785.t1	MTR_4g072330	63.636	77	26	1	1	77	1	75	6.45e-32	106
MS.gene057785.t1	MTR_4g072550	79.310	58	12	0	21	78	32	89	9.47e-32	106
MS.gene057785.t1	MTR_4g072610	67.532	77	19	1	1	77	1	71	1.41e-31	105
MS.gene057785.t1	MTR_4g072490	63.636	77	23	1	1	77	1	72	1.52e-31	105
MS.gene057785.t1	MTR_4g072830	64.935	77	25	1	1	77	1	75	1.67e-31	105
MS.gene057785.t1	MTR_4g072470	66.234	77	21	1	1	77	1	72	2.96e-31	104
MS.gene057785.t1	MTR_4g072630	62.338	77	25	1	1	77	1	73	4.33e-31	104
MS.gene057785.t1	MTR_4g072600	67.532	77	20	1	1	77	1	72	6.91e-31	103
MS.gene057785.t1	MTR_5g008350	62.338	77	24	1	1	77	1	72	1.08e-30	103
MS.gene057785.t1	MTR_4g072750	67.568	74	22	1	1	72	1	74	2.02e-30	102
MS.gene057785.t1	MTR_4g072740	62.338	77	24	1	1	77	1	72	4.26e-30	102
MS.gene057785.t1	MTR_4g072850	65.000	80	26	1	1	78	1	80	6.00e-30	101
MS.gene057785.t1	MTR_8g461420	66.234	77	26	0	1	77	1	77	6.98e-30	101
MS.gene057785.t1	MTR_3g084190	64.935	77	27	0	1	77	1	77	7.87e-30	101
MS.gene057785.t1	MTR_4g072820	65.823	79	20	2	1	78	1	73	8.93e-30	101
MS.gene057785.t1	MTR_3g084210	64.935	77	27	0	1	77	1	77	1.14e-29	100
MS.gene057785.t1	MTR_5g008370	82.456	57	10	0	22	78	22	78	1.15e-29	102
MS.gene057785.t1	MTR_4g072465	61.039	77	26	1	1	77	1	73	1.27e-29	100
MS.gene057785.t1	MTR_3g084160	63.636	77	28	0	1	77	1	77	1.50e-29	100
MS.gene057785.t1	MTR_4g072940	77.586	58	13	0	21	78	53	110	1.52e-29	101
MS.gene057785.t1	MTR_3g084230	64.935	77	27	0	1	77	1	77	2.30e-29	100
MS.gene057785.t1	MTR_4g072590	80.000	55	11	0	23	77	18	72	4.55e-29	99.4
MS.gene057785.t1	MTR_4g072650	63.636	77	22	1	1	77	1	71	5.16e-29	99.4
MS.gene057785.t1	MTR_4g072540	75.439	57	14	0	21	77	17	73	6.94e-29	99.0
MS.gene057785.t1	MTR_3g110045	65.385	78	25	1	2	77	59	136	2.27e-28	99.8
MS.gene057785.t1	MTR_4g072720	73.684	57	15	0	21	77	17	73	7.01e-28	96.3
MS.gene057785.t1	MTR_4g072670	73.684	57	15	0	21	77	17	73	1.72e-27	95.5
MS.gene057785.t1	MTR_3g084200	59.722	72	29	0	1	72	1	72	2.95e-27	95.1
MS.gene057785.t1	MTR_4g072380	58.974	78	28	1	1	78	1	74	7.41e-27	94.0
MS.gene057785.t1	MTR_4g072510	71.930	57	16	0	21	77	17	73	9.74e-27	93.6
MS.gene057785.t1	MTR_4g072770	61.538	78	20	2	1	78	1	68	1.10e-26	93.2
MS.gene057785.t1	MTR_4g072980	55.128	78	29	2	1	77	8	80	1.97e-25	90.5
MS.gene057785.t1	MTR_4g072450	74.545	55	14	0	23	77	18	72	3.97e-25	89.4
MS.gene057785.t1	MTR_4g072910	57.746	71	29	1	9	78	18	88	4.07e-25	89.7
MS.gene057785.t1	MTR_3g117640	72.727	55	14	1	24	77	33	87	5.44e-25	89.7
MS.gene057785.t1	MTR_4g072690	72.727	55	15	0	23	77	18	72	6.37e-25	89.0
MS.gene057785.t1	MTR_4g072560	54.930	71	31	1	8	77	15	85	1.73e-24	88.2
MS.gene057785.t1	MTR_4g072280	52.000	75	35	1	1	74	1	75	1.53e-22	82.8
MS.gene057785.t1	MTR_4g072290	50.633	79	24	2	1	78	1	65	1.73e-20	77.4
MS.gene057785.t1	MTR_6g077850	55.556	54	24	0	24	77	33	86	8.62e-20	76.3
MS.gene057785.t1	MTR_3g110035	59.615	52	21	0	26	77	72	123	1.40e-19	76.6
MS.gene057785.t1	MTR_3g117610	58.182	55	22	1	23	77	43	96	3.64e-19	75.1
MS.gene057785.t1	MTR_3g113310	53.571	56	25	1	23	78	40	94	1.15e-18	73.6
MS.gene057785.t1	MTR_4g072190	50.000	68	31	2	10	77	23	87	2.23e-18	72.8
MS.gene057785.t1	MTR_4g072930	53.571	56	22	1	27	78	43	98	3.59e-17	70.1
MS.gene057785.t1	MTR_5g021820	53.704	54	24	1	24	77	39	91	2.31e-16	67.8
MS.gene057785.t1	MTR_8g076040	53.704	54	24	1	24	77	40	92	5.01e-16	67.0
MS.gene057785.t1	MTR_2g043960	50.000	66	29	2	12	77	66	127	1.29e-14	64.7
MS.gene057785.t1	MTR_8g461210	44.444	72	39	1	6	77	16	86	2.60e-14	62.8
MS.gene057785.t1	MTR_4g124750	53.704	54	24	1	24	77	68	120	5.39e-14	62.8
MS.gene057785.t1	MTR_3g092220	40.506	79	41	3	1	77	12	86	6.17e-14	61.6
MS.gene057785.t1	MTR_7g118010	50.909	55	24	2	25	77	23	76	2.97e-13	59.7
MS.gene057785.t1	MTR_4g005310	51.724	58	27	1	21	77	58	115	5.78e-13	60.1
MS.gene057785.t1	MTR_3g105900	75.000	36	9	0	24	59	1171	1206	8.05e-13	62.0
MS.gene057785.t1	MTR_8g026730	50.909	55	24	2	25	77	27	80	9.32e-13	58.5
MS.gene057785.t1	MTR_1g009150	54.902	51	22	1	27	77	73	122	1.50e-12	59.7
MS.gene057785.t1	MTR_0388s0020	43.662	71	35	3	10	77	61	129	2.69e-12	58.5
MS.gene057785.t1	MTR_2g095430	48.276	58	25	2	24	77	44	100	3.06e-12	57.8
MS.gene057785.t1	MTR_1g024085	47.059	68	31	2	10	77	26	88	3.41e-12	58.2
MS.gene057785.t1	MTR_4g025830	46.296	54	25	2	27	77	81	133	1.80e-11	56.6
MS.gene057785.t1	MTR_4g005320	49.020	51	25	1	27	77	72	121	2.30e-11	56.2
MS.gene057785.t1	MTR_8g101520	41.333	75	39	2	7	77	28	101	3.15e-11	55.8
MS.gene057785.t1	MTR_7g104950	39.062	64	37	2	16	78	40	102	3.23e-11	55.1
MS.gene057785.t1	MTR_1g059770	45.098	51	27	1	28	78	52	101	8.78e-11	53.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057785.t1	AT2G21200	58.904	73	28	2	7	77	2	74	3.48e-27	94.7
MS.gene057785.t1	AT4G34770	59.740	77	29	1	4	78	14	90	1.09e-26	94.0
MS.gene057785.t1	AT4G38840	71.429	56	15	1	24	78	32	87	1.88e-26	93.2
MS.gene057785.t1	AT5G18020	56.410	78	32	2	1	77	1	77	2.31e-26	92.8
MS.gene057785.t1	AT5G18050	60.563	71	21	2	8	77	13	77	5.00e-26	91.7
MS.gene057785.t1	AT5G18060	61.972	71	20	2	8	77	13	77	5.46e-26	91.7
MS.gene057785.t1	AT5G18080	60.563	71	21	2	8	77	13	77	8.94e-26	91.3
MS.gene057785.t1	AT4G34800	58.333	72	27	1	9	77	5	76	2.48e-25	90.1
MS.gene057785.t1	AT4G38850	56.000	75	29	2	7	77	2	76	4.55e-25	89.4
MS.gene057785.t1	AT5G18030	59.155	71	20	2	8	77	13	75	4.83e-25	89.4
MS.gene057785.t1	AT4G38825	71.154	52	14	1	27	77	25	76	6.60e-25	89.0
MS.gene057785.t1	AT3G03840	55.844	77	29	2	3	77	8	81	8.78e-25	89.0
MS.gene057785.t1	AT5G18010	59.155	71	22	2	8	77	13	77	2.12e-24	87.8
MS.gene057785.t1	AT3G03850	55.844	77	27	2	3	77	8	79	3.06e-24	87.4
MS.gene057785.t1	AT4G34810	59.722	72	23	2	12	77	33	104	3.82e-24	87.8
MS.gene057785.t1	AT2G21210	45.783	83	40	2	1	78	1	83	6.47e-24	86.7
MS.gene057785.t1	AT2G21210	45.783	83	40	2	1	78	68	150	3.28e-23	87.0
MS.gene057785.t1	AT3G03820	56.164	73	26	2	8	77	13	82	1.34e-22	83.2
MS.gene057785.t1	AT3G03830	52.564	78	28	2	3	77	8	79	1.67e-22	82.8
MS.gene057785.t1	AT4G13790	44.304	79	42	1	1	77	1	79	1.79e-21	80.5
MS.gene057785.t1	AT4G34790	63.636	55	18	1	25	77	39	93	3.46e-20	77.4
MS.gene057785.t1	AT4G34780	48.718	78	34	2	3	77	9	83	2.79e-19	75.1
MS.gene057785.t1	AT4G38860	54.545	55	25	0	23	77	38	92	3.34e-18	72.4
MS.gene057785.t1	AT1G75580	58.182	55	22	1	23	77	42	95	1.20e-17	71.2
MS.gene057785.t1	AT4G34760	54.545	55	24	1	23	77	41	94	1.33e-17	70.9
MS.gene057785.t1	AT2G21220	54.545	55	24	1	23	77	38	91	1.44e-17	70.9
MS.gene057785.t1	AT1G19830	56.364	55	23	1	23	77	46	99	1.97e-16	68.2
MS.gene057785.t1	AT2G16580	50.909	55	26	1	23	77	42	95	4.46e-15	64.7
MS.gene057785.t1	AT3G20220	50.000	62	29	2	17	77	39	99	6.24e-15	64.3
MS.gene057785.t1	AT4G36110	51.852	54	25	1	24	77	39	91	6.33e-15	63.9
MS.gene057785.t1	AT2G18010	51.852	54	25	1	24	77	47	99	1.04e-14	63.5
MS.gene057785.t1	AT3G43120	51.852	54	25	1	24	77	78	130	2.37e-14	63.9
MS.gene057785.t1	AT5G20810	53.704	54	24	1	24	77	78	130	1.47e-13	62.0
MS.gene057785.t1	AT3G51200	51.923	52	24	1	26	77	41	91	1.59e-13	60.5
MS.gene057785.t1	AT5G20810	53.704	54	24	1	24	77	78	130	2.27e-13	62.0
MS.gene057785.t1	AT2G46690	41.026	78	33	3	3	77	11	78	8.00e-13	59.3
MS.gene057785.t1	AT5G66260	54.717	53	23	1	25	77	34	85	9.43e-13	58.5
MS.gene057785.t1	AT4G00880	53.571	56	23	2	24	77	27	81	7.56e-12	56.6
MS.gene057785.t1	AT3G12830	40.845	71	40	2	7	77	33	101	3.09e-11	55.5
MS.gene057785.t1	AT1G75590	48.148	54	27	1	24	77	41	93	3.84e-11	55.5
MS.gene057785.t1	AT2G37030	49.091	55	27	1	24	78	48	101	4.23e-11	54.7
MS.gene057785.t1	AT3G09870	50.000	56	25	2	24	77	44	98	4.42e-11	54.3
MS.gene057785.t1	AT3G61900	45.614	57	27	2	24	77	29	84	7.69e-11	54.3
MS.gene057785.t1	AT4G31320	49.020	51	25	1	27	77	82	131	7.91e-11	55.5

Find 22 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTCTCACTTCTACAGATTT+TGG	0.088811	4.4:-36569099	None:intergenic
TTCAATTACATACTATTCTA+AGG	0.187800	4.4:+36569049	MS.gene057785:CDS
AGTCAAACTCAAGAAGAATT+TGG	0.258998	4.4:+36569213	MS.gene057785:CDS
TGGTTGGTTCAAGTATGATA+TGG	0.316740	4.4:-36569172	None:intergenic
GGTTGGTTCAAGTATGATAT+GGG	0.445606	4.4:-36569171	None:intergenic
GTCACCATTCCTTGTACCTA+AGG	0.469405	4.4:+36569258	MS.gene057785:CDS
AAAATCTGTAGAAGTGAGAA+AGG	0.476102	4.4:+36569101	MS.gene057785:CDS
ATGTAGGAGAGGAGCAGAAG+AGG	0.513132	4.4:+36569139	MS.gene057785:CDS
TCAATTACATACTATTCTAA+GGG	0.530253	4.4:+36569050	MS.gene057785:CDS
GGATATGTTGCAGTGTATGT+AGG	0.540916	4.4:+36569123	MS.gene057785:CDS
TTTGGATATGATCATCCAAT+GGG	0.541874	4.4:+36569231	MS.gene057785:CDS
ATTTGGATATGATCATCCAA+TGG	0.556875	4.4:+36569230	MS.gene057785:CDS
TGTTGCAGTGTATGTAGGAG+AGG	0.573662	4.4:+36569128	MS.gene057785:CDS
AAATCTGTAGAAGTGAGAAA+GGG	0.578349	4.4:+36569102	MS.gene057785:CDS
GCAAGTCTTGAAACGATGGT+TGG	0.581681	4.4:-36569188	None:intergenic
CTCAGCAAGTCTTGAAACGA+TGG	0.585051	4.4:-36569192	None:intergenic
GGAATGGTGACACCACCCAT+TGG	0.588428	4.4:-36569246	None:intergenic
GGGAAGACACCTTAGGTACA+AGG	0.618624	4.4:-36569267	None:intergenic
GACACCTTAGGTACAAGGAA+TGG	0.624274	4.4:-36569262	None:intergenic
TATTCTAAGGGCATCAGTAG+CGG	0.642658	4.4:+36569062	MS.gene057785:CDS
GTTGGTTCAAGTATGATATG+GGG	0.673944	4.4:-36569170	None:intergenic
GGATATGATCATCCAATGGG+TGG	0.715103	4.4:+36569234	MS.gene057785:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TCAATTACATACTATTCTAA+GGG	+	chr4.4:36569050-36569069	MS.gene057785:CDS	20.0%
!!	TTCAATTACATACTATTCTA+AGG	+	chr4.4:36569049-36569068	MS.gene057785:CDS	20.0%
	AAAATCTGTAGAAGTGAGAA+AGG	+	chr4.4:36569101-36569120	MS.gene057785:CDS	30.0%
	AAATCTGTAGAAGTGAGAAA+GGG	+	chr4.4:36569102-36569121	MS.gene057785:CDS	30.0%
	AGTCAAACTCAAGAAGAATT+TGG	+	chr4.4:36569213-36569232	MS.gene057785:CDS	30.0%
	ATTTGGATATGATCATCCAA+TGG	+	chr4.4:36569230-36569249	MS.gene057785:CDS	30.0%
	TTTGGATATGATCATCCAAT+GGG	+	chr4.4:36569231-36569250	MS.gene057785:CDS	30.0%
!	TTCTCACTTCTACAGATTTT+GGG	-	chr4.4:36569101-36569120	None:intergenic	30.0%
!	TTTCTCACTTCTACAGATTT+TGG	-	chr4.4:36569102-36569121	None:intergenic	30.0%
!	GGTTGGTTCAAGTATGATAT+GGG	-	chr4.4:36569174-36569193	None:intergenic	35.0%
!	GTTGGTTCAAGTATGATATG+GGG	-	chr4.4:36569173-36569192	None:intergenic	35.0%
!	TCTCACTTCTACAGATTTTG+GGG	-	chr4.4:36569100-36569119	None:intergenic	35.0%
!	TGGTTGGTTCAAGTATGATA+TGG	-	chr4.4:36569175-36569194	None:intergenic	35.0%
	GGATATGTTGCAGTGTATGT+AGG	+	chr4.4:36569123-36569142	MS.gene057785:CDS	40.0%
!!	TATTCTAAGGGCATCAGTAG+CGG	+	chr4.4:36569062-36569081	MS.gene057785:CDS	40.0%
!!!	ACTTCTACAGATTTTGGGGT+TGG	-	chr4.4:36569096-36569115	None:intergenic	40.0%
	CTCAGCAAGTCTTGAAACGA+TGG	-	chr4.4:36569195-36569214	None:intergenic	45.0%
	GCAAGTCTTGAAACGATGGT+TGG	-	chr4.4:36569191-36569210	None:intergenic	45.0%
	GGATATGATCATCCAATGGG+TGG	+	chr4.4:36569234-36569253	MS.gene057785:CDS	45.0%
	TGTTGCAGTGTATGTAGGAG+AGG	+	chr4.4:36569128-36569147	MS.gene057785:CDS	45.0%
	ATGTAGGAGAGGAGCAGAAG+AGG	+	chr4.4:36569139-36569158	MS.gene057785:CDS	50.0%
	GGAATGGTGACACCACCCAT+TGG	-	chr4.4:36569249-36569268	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	36569042	36569278	36569042	ID=MS.gene057785
chr4.4	mRNA	36569042	36569278	36569042	ID=MS.gene057785.t1;Parent=MS.gene057785
chr4.4	exon	36569042	36569278	36569042	ID=MS.gene057785.t1.exon1;Parent=MS.gene057785.t1
chr4.4	CDS	36569042	36569278	36569042	ID=cds.MS.gene057785.t1;Parent=MS.gene057785.t1

Gene Sequence	>MS.gene057785 ATGGGTTTTCAATTACATACTATTCTAAGGGCATCAGTAGCGGCTAGACAACCAACCCCAAAATCTGTAGAAGTGAGAAAGGGATATGTTGCAGTGTATGTAGGAGAGGAGCAGAAGAGGTTTGTGATCCCCATATCATACTTGAACCAACCATCGTTTCAAGACTTGCTGAGTCAAACTCAAGAAGAATTTGGATATGATCATCCAATGGGTGGTGTCACCATTCCTTGTACCTAA
Protein sequence	>MS.gene057785.t1 MGFQLHTILRASVAARQPTPKSVEVRKGYVAVYVGEEQKRFVIPISYLNQPSFQDLLSQTQEEFGYDHPMGGVTIPCT