AlfalfaGEDB | MS.gene057791

M. sativa cultivar XinJiangDaYe / MS.gene057791

KEGG: K14488
GO: GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
PFAM: PF02519.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057791.t1	AES89341.1	93.2	88	4	1	1	88	1	86	3.90E-37	163.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057791.t1	P33080	68.5	92	23	2	1	88	1	90	2.0e-29	129.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057791.t1	G7JM98	93.2	88	4	1	1	88	1	86	2.8e-37	163.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene68165	MS.gene057791	PPI
MS.gene057791	MS.gene014669	PPI
MS.gene057791	MS.gene057808	PPI
MS.gene057791	MS.gene002380	PPI
MS.gene50763	MS.gene057791	PPI
MS.gene01358	MS.gene057791	PPI
MS.gene057791	MS.gene68163	PPI
MS.gene50659	MS.gene057791	PPI
MS.gene057791	MS.gene75315	PPI
MS.gene057790	MS.gene057791	PPI
MS.gene057791	MS.gene68164	PPI
MS.gene062952	MS.gene057791	PPI
MS.gene057791	MS.gene68759	PPI
MS.gene057791	MS.gene79889	PPI
MS.gene003624	MS.gene057791	PPI
MS.gene031199	MS.gene057791	PPI
MS.gene057791	MS.gene014222	PPI
MS.gene057791	MS.gene79888	PPI
MS.gene23370	MS.gene057791	PPI
MS.gene77529	MS.gene057791	PPI
MS.gene68761	MS.gene057791	PPI
MS.gene014667	MS.gene057791	PPI
MS.gene68760	MS.gene057791	PPI
MS.gene057791	MS.gene75678	PPI
MS.gene75676	MS.gene057791	PPI
MS.gene50665	MS.gene057791	PPI
MS.gene057791	MS.gene014223	PPI
MS.gene003586	MS.gene057791	PPI
MS.gene057791	MS.gene79885	PPI
MS.gene057778	MS.gene057791	PPI
MS.gene062988	MS.gene057791	PPI
MS.gene75673	MS.gene057791	PPI
MS.gene045366	MS.gene057791	PPI
MS.gene062963	MS.gene057791	PPI
MS.gene75674	MS.gene057791	PPI
MS.gene031200	MS.gene057791	PPI
MS.gene045365	MS.gene057791	PPI
MS.gene003602	MS.gene057791	PPI
MS.gene23372	MS.gene057791	PPI
MS.gene75316	MS.gene057791	PPI
MS.gene003577	MS.gene057791	PPI
MS.gene062984	MS.gene057791	PPI
MS.gene057791	MS.gene014670	PPI
MS.gene23371	MS.gene057791	PPI
MS.gene031201	MS.gene057791	PPI
MS.gene057791	MS.gene75314	PPI
MS.gene057791	MS.gene01764	PPI
MS.gene057791	MS.gene75679	PPI
MS.gene045362	MS.gene057791	PPI
MS.gene77523	MS.gene057791	PPI
MS.gene003587	MS.gene057791	PPI
MS.gene063112	MS.gene057791	PPI
MS.gene057791	MS.gene031198	PPI
MS.gene057791	MS.gene057797	PPI
MS.gene057791	MS.gene003614	PPI
MS.gene057791	MS.gene50764	PPI
MS.gene057791	MS.gene77528	PPI
MS.gene79890	MS.gene057791	PPI
MS.gene057791	MS.gene014671	PPI
MS.gene003615	MS.gene057791	PPI
MS.gene057791	MS.gene003596	PPI
MS.gene062973	MS.gene057791	PPI
MS.gene047517	MS.gene057791	PPI
MS.gene057791	MS.gene75672	PPI
MS.gene045360	MS.gene057791	PPI
MS.gene057791	MS.gene50654	PPI
MS.gene003627	MS.gene057791	PPI
MS.gene057791	MS.gene062974	PPI
MS.gene057791	MS.gene003583	PPI
MS.gene057791	MS.gene062951	PPI
MS.gene057791	MS.gene057801	PPI
MS.gene057791	MS.gene77527	PPI
MS.gene057791	MS.gene77524	PPI
MS.gene057791	MS.gene68162	PPI
MS.gene50687	MS.gene057791	PPI
MS.gene40327	MS.gene057791	PPI
MS.gene75677	MS.gene057791	PPI
MS.gene057791	MS.gene045363	PPI
MS.gene057791	MS.gene75675	PPI
MS.gene057791	MS.gene79887	PPI
MS.gene057795	MS.gene057791	PPI
MS.gene057791	MS.gene50652	PPI
MS.gene045361	MS.gene057791	PPI
MS.gene057791	MS.gene40325	PPI
MS.gene50675	MS.gene057791	PPI
MS.gene003591	MS.gene057791	PPI
MS.gene062979	MS.gene057791	PPI
MS.gene003590	MS.gene057791	PPI
MS.gene045368	MS.gene057791	PPI
MS.gene062943	MS.gene057791	PPI
MS.gene057791	MS.gene50684	PPI
MS.gene057791	MS.gene014668	PPI
MS.gene003608	MS.gene057791	PPI
MS.gene057791	MS.gene79886	PPI
MS.gene057791	MS.gene50765	PPI
MS.gene40326	MS.gene057791	PPI
MS.gene77526	MS.gene057791	PPI
MS.gene50676	MS.gene057791	PPI
MS.gene057818	MS.gene057791	PPI
MS.gene50766	MS.gene057791	PPI
MS.gene003594	MS.gene057791	PPI
MS.gene057791	MS.gene79891	PPI
MS.gene057791	MS.gene045367	PPI
MS.gene057791	MS.gene045364	PPI
MS.gene50677	MS.gene057791	PPI
MS.gene003625	MS.gene057791	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057791.t1	MTR_4g072490	89.773	88	6	2	1	88	1	85	7.47e-53	159
MS.gene057791.t1	MTR_4g072630	86.364	88	10	1	1	88	1	86	2.52e-52	158
MS.gene057791.t1	MTR_4g072465	85.227	88	11	1	1	88	1	86	7.56e-52	157
MS.gene057791.t1	MTR_4g072740	87.500	88	8	2	1	88	1	85	6.10e-51	154
MS.gene057791.t1	MTR_4g072720	93.182	88	4	1	1	88	1	86	6.55e-48	147
MS.gene057791.t1	MTR_4g072470	81.818	88	13	2	1	88	1	85	3.12e-47	145
MS.gene057791.t1	MTR_4g072540	89.773	88	7	1	1	88	1	86	1.84e-46	143
MS.gene057791.t1	MTR_4g072510	88.636	88	8	1	1	88	1	86	5.59e-46	142
MS.gene057791.t1	MTR_4g072670	86.364	88	10	1	1	88	1	86	1.14e-45	141
MS.gene057791.t1	MTR_4g072580	79.545	88	15	2	1	88	1	85	1.48e-45	141
MS.gene057791.t1	MTR_4g072370	77.778	90	16	2	1	88	1	88	1.71e-45	141
MS.gene057791.t1	MTR_4g072830	80.000	90	12	3	1	87	1	87	3.90e-44	137
MS.gene057791.t1	MTR_4g072600	76.136	88	18	2	1	88	1	85	8.05e-43	134
MS.gene057791.t1	MTR_3g084170	72.043	93	18	3	1	88	1	90	3.48e-42	133
MS.gene057791.t1	MTR_3g084150	71.739	92	20	2	1	88	1	90	3.84e-42	132
MS.gene057791.t1	MTR_4g072610	75.000	88	18	2	1	88	1	84	7.33e-42	132
MS.gene057791.t1	MTR_4g072330	71.429	91	20	3	1	88	1	88	2.47e-41	130
MS.gene057791.t1	MTR_3g084180	70.652	92	21	2	1	88	1	90	3.49e-41	130
MS.gene057791.t1	MTR_3g084240	70.968	93	19	3	1	88	1	90	3.52e-41	130
MS.gene057791.t1	MTR_4g072650	75.000	88	18	2	1	88	1	84	4.06e-41	130
MS.gene057791.t1	MTR_8g461410	69.565	92	22	2	1	88	1	90	5.35e-41	130
MS.gene057791.t1	MTR_4g072590	80.682	88	14	2	1	88	1	85	2.97e-40	127
MS.gene057791.t1	MTR_4g072870	69.149	94	20	3	1	88	1	91	4.27e-40	127
MS.gene057791.t1	MTR_3g084220	68.817	93	21	3	1	88	1	90	9.37e-40	127
MS.gene057791.t1	MTR_4g050700	68.817	93	22	2	1	88	1	91	1.67e-39	126
MS.gene057791.t1	MTR_2g012410	68.817	93	22	2	1	88	1	91	1.67e-39	126
MS.gene057791.t1	MTR_5g008350	80.519	77	12	2	1	77	1	74	2.50e-39	125
MS.gene057791.t1	MTR_4g072880	71.277	94	18	3	1	88	1	91	3.30e-39	125
MS.gene057791.t1	MTR_4g072690	78.409	88	16	2	1	88	1	85	5.15e-39	124
MS.gene057791.t1	MTR_3g110032	69.149	94	20	3	1	88	1	91	5.17e-39	125
MS.gene057791.t1	MTR_4g072310	67.742	93	23	2	1	88	1	91	5.46e-39	125
MS.gene057791.t1	MTR_4g072480	67.391	92	24	2	1	88	1	90	6.82e-39	124
MS.gene057791.t1	MTR_4g072380	74.157	89	19	3	1	88	1	86	7.75e-39	124
MS.gene057791.t1	MTR_3g110038	67.742	93	22	3	1	88	1	90	9.07e-39	124
MS.gene057791.t1	MTR_4g072450	78.409	88	16	2	1	88	1	85	1.76e-38	123
MS.gene057791.t1	MTR_4g072250	69.892	93	20	3	1	88	1	90	6.25e-38	122
MS.gene057791.t1	MTR_3g110042	68.132	91	23	2	1	87	1	89	1.25e-37	121
MS.gene057791.t1	MTR_4g072620	64.130	92	27	2	1	88	1	90	1.49e-37	121
MS.gene057791.t1	MTR_4g072850	69.149	94	21	2	1	88	1	92	3.51e-37	120
MS.gene057791.t1	MTR_4g072680	65.217	92	26	2	1	88	1	90	3.90e-37	120
MS.gene057791.t1	MTR_4g072820	71.910	89	20	3	1	88	1	85	4.26e-37	120
MS.gene057791.t1	MTR_4g072270	65.591	93	25	2	1	88	1	91	5.23e-37	120
MS.gene057791.t1	MTR_4g072240	63.441	93	27	2	1	88	1	91	5.94e-37	119
MS.gene057791.t1	MTR_4g072730	64.130	92	27	2	1	88	1	90	1.28e-36	119
MS.gene057791.t1	MTR_3g084260	63.441	93	27	2	1	88	1	91	1.91e-36	118
MS.gene057791.t1	MTR_4g072860	68.085	94	21	3	1	88	1	91	2.92e-36	118
MS.gene057791.t1	MTR_4g072570	64.130	92	27	2	1	88	1	90	3.82e-36	117
MS.gene057791.t1	MTR_3g084250	65.217	92	26	2	1	88	1	90	4.50e-36	117
MS.gene057791.t1	MTR_4g072220	62.366	93	28	2	1	88	1	91	5.46e-36	117
MS.gene057791.t1	MTR_4g072890	63.441	93	27	3	1	88	1	91	8.17e-36	117
MS.gene057791.t1	MTR_4g072500	65.217	92	26	2	1	88	1	90	8.41e-36	117
MS.gene057791.t1	MTR_4g072300	61.290	93	29	2	1	88	1	91	1.02e-35	116
MS.gene057791.t1	MTR_3g117630	74.667	75	17	1	14	88	18	90	1.06e-35	116
MS.gene057791.t1	MTR_4g072460	63.043	92	28	2	1	88	1	90	1.30e-35	116
MS.gene057791.t1	MTR_4g072260	62.366	93	28	3	1	88	1	91	3.09e-35	115
MS.gene057791.t1	MTR_4g072230	65.591	93	23	3	1	88	1	89	5.63e-35	114
MS.gene057791.t1	MTR_3g084190	66.304	92	25	2	1	88	1	90	9.59e-35	114
MS.gene057791.t1	MTR_4g072530	63.043	92	28	2	1	88	1	90	1.10e-34	114
MS.gene057791.t1	MTR_4g072640	63.333	90	31	1	1	88	1	90	1.50e-34	114
MS.gene057791.t1	MTR_3g084210	63.043	92	28	2	1	88	1	90	2.00e-34	113
MS.gene057791.t1	MTR_8g461420	65.217	92	26	2	1	88	1	90	2.49e-34	113
MS.gene057791.t1	MTR_4g072660	62.500	88	30	2	1	88	1	85	3.27e-34	112
MS.gene057791.t1	MTR_3g084230	64.130	92	27	2	1	88	1	90	1.33e-33	111
MS.gene057791.t1	MTR_3g084160	65.217	92	26	2	1	88	1	90	1.68e-33	111
MS.gene057791.t1	MTR_3g110045	65.556	90	25	2	3	88	62	149	1.94e-33	112
MS.gene057791.t1	MTR_5g008370	64.130	92	27	2	1	88	1	90	8.44e-33	110
MS.gene057791.t1	MTR_5g008360	65.000	80	26	1	9	88	4	81	9.22e-33	108
MS.gene057791.t1	MTR_4g072940	65.217	92	24	2	3	88	33	122	8.21e-32	107
MS.gene057791.t1	MTR_4g072560	53.465	101	29	4	1	87	1	97	7.27e-30	102
MS.gene057791.t1	MTR_4g072770	60.227	88	27	2	1	88	1	80	6.92e-29	99.0
MS.gene057791.t1	MTR_3g084200	68.000	75	16	3	1	70	1	72	1.29e-28	99.4
MS.gene057791.t1	MTR_4g072980	53.191	94	35	3	1	87	1	92	2.21e-28	98.2
MS.gene057791.t1	MTR_4g072550	60.494	81	29	1	11	88	21	101	4.05e-28	97.8
MS.gene057791.t1	MTR_4g072910	60.811	74	27	1	15	88	29	100	5.36e-27	95.1
MS.gene057791.t1	MTR_4g072290	56.818	88	27	2	1	88	1	77	1.42e-26	93.2
MS.gene057791.t1	MTR_3g117640	60.000	70	25	2	20	88	33	100	1.52e-25	91.3
MS.gene057791.t1	MTR_3g110035	62.500	64	24	0	25	88	73	136	1.05e-24	90.1
MS.gene057791.t1	MTR_6g077850	50.704	71	35	0	18	88	29	99	7.40e-22	82.0
MS.gene057791.t1	MTR_4g072750	68.421	57	16	1	14	70	20	74	1.31e-21	80.9
MS.gene057791.t1	MTR_4g072280	57.143	77	26	3	1	72	1	75	4.49e-21	79.3
MS.gene057791.t1	MTR_3g117610	53.731	67	28	2	19	85	43	106	9.88e-20	77.0
MS.gene057791.t1	MTR_3g113310	50.725	69	31	2	19	87	40	105	1.07e-19	76.6
MS.gene057791.t1	MTR_4g072930	50.000	68	30	1	25	88	43	110	7.77e-19	74.7
MS.gene057791.t1	MTR_4g072190	52.239	67	29	2	19	85	34	97	9.84e-19	73.9
MS.gene057791.t1	MTR_4g072840	49.438	89	15	4	1	88	1	60	3.48e-17	68.9
MS.gene057791.t1	MTR_8g461400	46.237	93	16	4	1	88	1	64	4.75e-17	68.9
MS.gene057791.t1	MTR_5g021820	50.000	68	31	2	20	87	39	103	1.62e-16	68.6
MS.gene057791.t1	MTR_8g076040	51.515	66	29	2	20	85	40	102	8.04e-16	66.6
MS.gene057791.t1	MTR_2g043960	54.098	61	25	2	20	80	75	132	2.24e-15	67.0
MS.gene057791.t1	MTR_1g009150	52.113	71	31	2	13	83	63	130	3.07e-15	67.0
MS.gene057791.t1	MTR_4g124750	52.459	61	26	2	20	80	68	125	5.71e-15	65.9
MS.gene057791.t1	MTR_5g008380	84.375	32	5	0	57	88	246	277	4.85e-14	65.5
MS.gene057791.t1	MTR_1g094750	51.562	64	28	2	20	83	49	109	8.55e-14	62.0
MS.gene057791.t1	MTR_7g118010	47.692	65	33	1	21	85	23	86	1.51e-13	60.8
MS.gene057791.t1	MTR_4g005310	46.875	64	31	2	21	83	62	123	3.70e-13	61.2
MS.gene057791.t1	MTR_0388s0020	43.038	79	39	3	12	87	66	141	5.08e-13	60.8
MS.gene057791.t1	MTR_4g005320	49.206	63	29	2	21	83	70	129	1.49e-12	59.7
MS.gene057791.t1	MTR_3g105900	65.909	44	13	1	20	63	1171	1212	1.53e-12	61.6
MS.gene057791.t1	MTR_4g025830	45.714	70	32	3	21	87	79	145	1.94e-12	59.3
MS.gene057791.t1	MTR_1g024085	45.205	73	35	3	10	80	24	93	5.70e-12	57.8
MS.gene057791.t1	MTR_3g109160	48.649	74	33	3	10	83	60	128	5.74e-12	58.2
MS.gene057791.t1	MTR_1g061570	43.210	81	41	3	5	83	1	78	5.97e-12	57.4
MS.gene057791.t1	MTR_7g101740	45.455	66	33	2	20	85	39	101	6.60e-12	57.0
MS.gene057791.t1	MTR_3g092220	45.902	61	32	1	20	80	32	91	8.18e-12	56.6
MS.gene057791.t1	MTR_1g063950	41.429	70	38	2	14	83	41	107	1.03e-11	56.6
MS.gene057791.t1	MTR_8g026730	44.444	63	34	1	21	83	27	88	2.23e-11	55.5
MS.gene057791.t1	MTR_4g072160	46.970	66	32	2	18	83	34	96	7.31e-11	54.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene057791.t1	AT4G38840	71.429	70	17	2	20	88	32	99	5.87e-31	105
MS.gene057791.t1	AT2G21210	56.627	83	32	2	8	88	15	95	7.71e-29	99.8
MS.gene057791.t1	AT5G18060	61.333	75	27	2	15	88	17	90	1.65e-28	98.6
MS.gene057791.t1	AT2G21210	56.627	83	32	2	8	88	82	162	4.00e-28	99.8
MS.gene057791.t1	AT4G38825	62.162	74	25	2	16	88	18	89	4.69e-28	97.4
MS.gene057791.t1	AT5G18080	59.211	76	30	1	14	88	15	90	5.50e-28	97.1
MS.gene057791.t1	AT5G18020	59.211	76	28	2	14	88	17	90	7.35e-28	97.1
MS.gene057791.t1	AT4G34800	54.651	86	34	2	1	83	1	84	9.22e-28	96.7
MS.gene057791.t1	AT5G18030	60.526	76	27	2	14	88	15	88	1.12e-27	96.3
MS.gene057791.t1	AT5G18050	57.895	76	31	1	14	88	15	90	1.31e-27	96.3
MS.gene057791.t1	AT2G21200	62.162	74	25	2	15	87	15	86	2.72e-27	95.5
MS.gene057791.t1	AT4G38850	57.143	84	26	3	8	88	13	89	6.86e-27	94.4
MS.gene057791.t1	AT5G18010	56.579	76	32	1	14	88	15	90	2.30e-26	93.2
MS.gene057791.t1	AT4G34810	58.750	80	27	2	10	85	37	114	2.87e-26	93.6
MS.gene057791.t1	AT3G03820	55.696	79	30	2	13	88	19	95	9.02e-25	89.4
MS.gene057791.t1	AT3G03850	63.636	66	22	1	25	88	27	92	1.13e-24	89.0
MS.gene057791.t1	AT3G03840	55.128	78	31	2	13	88	19	94	2.77e-24	87.8
MS.gene057791.t1	AT4G34770	54.651	86	35	2	5	88	19	102	3.91e-24	87.8
MS.gene057791.t1	AT4G13790	48.276	87	40	3	3	87	8	91	2.59e-23	85.5
MS.gene057791.t1	AT3G03830	59.701	67	24	1	25	88	26	92	7.57e-23	84.3
MS.gene057791.t1	AT4G34790	62.319	69	21	3	21	86	39	105	1.09e-21	81.6
MS.gene057791.t1	AT4G34780	56.716	67	26	1	25	88	30	96	9.61e-21	79.3
MS.gene057791.t1	AT4G38860	51.429	70	32	1	19	88	38	105	2.67e-20	78.2
MS.gene057791.t1	AT2G21220	52.239	67	29	2	19	85	38	101	1.19e-18	73.9
MS.gene057791.t1	AT1G75580	52.174	69	30	2	19	87	42	107	3.27e-18	72.8
MS.gene057791.t1	AT4G34760	52.239	67	29	2	19	85	41	104	7.37e-18	72.0
MS.gene057791.t1	AT2G16580	52.239	67	29	2	19	85	42	105	1.93e-17	70.9
MS.gene057791.t1	AT4G36110	42.683	82	44	2	4	85	23	101	2.46e-16	68.2
MS.gene057791.t1	AT2G18010	50.000	66	30	2	20	85	47	109	8.58e-16	67.0
MS.gene057791.t1	AT3G43120	52.459	61	26	2	20	80	78	135	1.35e-15	67.8
MS.gene057791.t1	AT1G19830	52.113	71	30	3	19	88	46	113	3.33e-15	65.5
MS.gene057791.t1	AT5G20810	54.098	61	25	2	20	80	78	135	4.96e-15	66.2
MS.gene057791.t1	AT3G51200	43.373	83	41	3	5	83	19	99	6.38e-15	64.7
MS.gene057791.t1	AT5G20810	54.098	61	25	2	20	80	78	135	6.91e-15	66.2
MS.gene057791.t1	AT3G20220	50.000	60	28	2	25	83	49	107	1.38e-13	61.2
MS.gene057791.t1	AT5G66260	40.000	85	48	2	4	88	17	98	7.23e-13	58.9
MS.gene057791.t1	AT4G34750	43.939	66	34	2	20	85	36	98	5.53e-12	58.2
MS.gene057791.t1	AT4G34750	43.939	66	34	2	20	85	36	98	5.53e-12	58.2
MS.gene057791.t1	AT2G37030	46.154	65	32	2	20	84	48	109	1.37e-11	56.6
MS.gene057791.t1	AT3G61900	40.698	86	41	4	10	88	15	97	1.47e-11	56.6
MS.gene057791.t1	AT1G75590	43.662	71	37	2	12	80	29	98	2.45e-11	56.6
MS.gene057791.t1	AT2G46690	41.772	79	40	3	5	80	8	83	3.22e-11	55.5
MS.gene057791.t1	AT5G10990	45.902	61	30	2	20	80	40	97	4.45e-11	55.5
MS.gene057791.t1	AT4G31320	44.286	70	36	2	14	83	73	139	5.02e-11	56.2
MS.gene057791.t1	AT2G24400	45.714	70	35	2	14	83	57	123	7.31e-11	55.5

Find 24 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGGCTTCGCTCAACAATTCT+TGG	0.246989	4.4:-36656187	None:intergenic
ACAATTCTTGGAATGAAGTT+TGG	0.300739	4.4:-36656175	None:intergenic
GCTAGATATCCCTGACCCTT+TGG	0.380716	4.4:-36656098	None:intergenic
TGTGGACGTTCCAAAGGGTC+AGG	0.454987	4.4:+36656088	MS.gene057791:CDS
GTCATATTCAAATTGTTCCT+CGG	0.462990	4.4:-36656207	None:intergenic
GTTTGGTTCAAATATGAGAC+AGG	0.500220	4.4:-36656158	None:intergenic
AAATGCTGTGGACGTTCCAA+AGG	0.522821	4.4:+36656082	MS.gene057791:CDS
TTTGAATATGACCATCCAAT+GGG	0.542663	4.4:+36656218	MS.gene057791:CDS
AAGAAAATGTCTTCTCTACA+AGG	0.543989	4.4:-36656254	None:intergenic
GCTAATTCAATTTAAGTGAG+AGG	0.558843	4.4:-36656285	None:intergenic
ATTTGAATATGACCATCCAA+TGG	0.559485	4.4:+36656217	MS.gene057791:CDS
GGCATCATCTTCAAATGCTG+TGG	0.565227	4.4:+36656070	MS.gene057791:CDS
GCCCGTGGTCTGATGATACT+AGG	0.578678	4.4:-36656050	None:intergenic
ACCTAGTATCATCAGACCAC+GGG	0.583683	4.4:+36656049	MS.gene057791:CDS
TAGTGAGACCACCCATTGGA+TGG	0.596413	4.4:-36656229	None:intergenic
AATGCTGTGGACGTTCCAAA+GGG	0.614561	4.4:+36656083	MS.gene057791:CDS
GGAATAGTGAGACCACCCAT+TGG	0.616341	4.4:-36656233	None:intergenic
AGAATTGTTGAGCGAAGCCG+AGG	0.622670	4.4:+36656190	MS.gene057791:CDS
GTGGACGTTCCAAAGGGTCA+GGG	0.638741	4.4:+36656089	MS.gene057791:CDS
GAATATGACCATCCAATGGG+TGG	0.646056	4.4:+36656221	MS.gene057791:CDS
TACCTAGTATCATCAGACCA+CGG	0.670557	4.4:+36656048	MS.gene057791:CDS
GCATTTGAAGATGATGCCCG+TGG	0.691558	4.4:-36656065	None:intergenic
GGATATCTAGCAGTGTACGT+AGG	0.698206	4.4:+36656110	MS.gene057791:CDS
ACGTAGGAGAGAAAATGAAG+CGG	0.744637	4.4:+36656126	MS.gene057791:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAGAAAATGTCTTCTCTACA+AGG	-	chr4.4:36656257-36656276	None:intergenic	30.0%
	ACAATTCTTGGAATGAAGTT+TGG	-	chr4.4:36656178-36656197	None:intergenic	30.0%
	ATTTGAATATGACCATCCAA+TGG	+	chr4.4:36656217-36656236	MS.gene057791:CDS	30.0%
	GTCATATTCAAATTGTTCCT+CGG	-	chr4.4:36656210-36656229	None:intergenic	30.0%
	TTTGAATATGACCATCCAAT+GGG	+	chr4.4:36656218-36656237	MS.gene057791:CDS	30.0%
!	GTTTGGTTCAAATATGAGAC+AGG	-	chr4.4:36656161-36656180	None:intergenic	35.0%
	ACGTAGGAGAGAAAATGAAG+CGG	+	chr4.4:36656126-36656145	MS.gene057791:CDS	40.0%
	TACCTAGTATCATCAGACCA+CGG	+	chr4.4:36656048-36656067	MS.gene057791:CDS	40.0%
	AAATGCTGTGGACGTTCCAA+AGG	+	chr4.4:36656082-36656101	MS.gene057791:CDS	45.0%
	AATGCTGTGGACGTTCCAAA+GGG	+	chr4.4:36656083-36656102	MS.gene057791:CDS	45.0%
	ACCTAGTATCATCAGACCAC+GGG	+	chr4.4:36656049-36656068	MS.gene057791:CDS	45.0%
	GAATATGACCATCCAATGGG+TGG	+	chr4.4:36656221-36656240	MS.gene057791:CDS	45.0%
	GGATATCTAGCAGTGTACGT+AGG	+	chr4.4:36656110-36656129	MS.gene057791:CDS	45.0%
	GGCATCATCTTCAAATGCTG+TGG	+	chr4.4:36656070-36656089	MS.gene057791:CDS	45.0%
	AGAATTGTTGAGCGAAGCCG+AGG	+	chr4.4:36656190-36656209	MS.gene057791:CDS	50.0%
	CGGCTTCGCTCAACAATTCT+TGG	-	chr4.4:36656190-36656209	None:intergenic	50.0%
	GCATTTGAAGATGATGCCCG+TGG	-	chr4.4:36656068-36656087	None:intergenic	50.0%
	GCTAGATATCCCTGACCCTT+TGG	-	chr4.4:36656101-36656120	None:intergenic	50.0%
	GGAATAGTGAGACCACCCAT+TGG	-	chr4.4:36656236-36656255	None:intergenic	50.0%
	TAGTGAGACCACCCATTGGA+TGG	-	chr4.4:36656232-36656251	None:intergenic	50.0%
	GCCCGTGGTCTGATGATACT+AGG	-	chr4.4:36656053-36656072	None:intergenic	55.0%
	GTGGACGTTCCAAAGGGTCA+GGG	+	chr4.4:36656089-36656108	MS.gene057791:CDS	55.0%
	TGTGGACGTTCCAAAGGGTC+AGG	+	chr4.4:36656088-36656107	MS.gene057791:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	36656035	36656301	36656035	ID=MS.gene057791
chr4.4	mRNA	36656035	36656301	36656035	ID=MS.gene057791.t1;Parent=MS.gene057791
chr4.4	exon	36656035	36656301	36656035	ID=MS.gene057791.t1.exon1;Parent=MS.gene057791.t1
chr4.4	CDS	36656035	36656301	36656035	ID=cds.MS.gene057791.t1;Parent=MS.gene057791.t1

Gene Sequence	>MS.gene057791 ATGGGTTTTCGTTTACCTAGTATCATCAGACCACGGGCATCATCTTCAAATGCTGTGGACGTTCCAAAGGGTCAGGGATATCTAGCAGTGTACGTAGGAGAGAAAATGAAGCGGTTTGTGATTCCTGTCTCATATTTGAACCAAACTTCATTCCAAGAATTGTTGAGCGAAGCCGAGGAACAATTTGAATATGACCATCCAATGGGTGGTCTCACTATTCCTTGTAGAGAAGACATTTTCTTAGATATCACCTCTCACTTAAATTGA
Protein sequence	>MS.gene057791.t1 MGFRLPSIIRPRASSSNAVDVPKGQGYLAVYVGEKMKRFVIPVSYLNQTSFQELLSEAEEQFEYDHPMGGLTIPCREDIFLDITSHLN