AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene35375


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35375.t1 MTR_2g085025 98.851 174 2 0 1 174 1 174 1.74e-123 345
MS.gene35375.t1 MTR_7g104020 67.742 155 48 1 20 174 14 166 1.10e-61 188
MS.gene35375.t1 MTR_7g104020 66.456 158 48 2 20 174 14 169 1.27e-59 183
MS.gene35375.t1 MTR_1g492930 56.329 158 68 1 17 173 1 158 3.93e-51 161
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35375.t1 AT2G27740 59.649 171 68 1 1 171 1 170 4.65e-68 205
MS.gene35375.t1 AT2G36410 67.647 136 44 0 38 173 57 192 2.01e-61 189
MS.gene35375.t1 AT3G52920 68.889 135 42 0 38 172 28 162 3.93e-61 187
MS.gene35375.t1 AT3G09980 70.139 144 43 0 31 174 35 178 9.03e-60 184
MS.gene35375.t1 AT2G36410 66.187 139 44 1 38 173 57 195 2.42e-59 183
MS.gene35375.t1 AT3G52920 67.391 138 42 1 38 172 28 165 6.10e-59 182
MS.gene35375.t1 AT5G03660 66.418 134 45 0 38 171 36 169 2.16e-49 157
MS.gene35375.t1 AT3G52900 51.974 152 73 0 23 174 13 164 1.47e-48 155
MS.gene35375.t1 AT2G36355 51.299 154 69 2 22 173 5 154 1.67e-44 144
MS.gene35375.t1 AT2G27740 53.659 123 56 1 1 123 1 122 1.12e-41 136
MS.gene35375.t1 AT5G03660 63.793 116 42 0 38 153 36 151 8.52e-40 132
MS.gene35375.t1 AT2G36410 66.279 86 29 0 38 123 57 142 3.19e-35 120
MS.gene35375.t1 AT5G03660 53.731 134 40 1 38 171 36 147 9.69e-32 112
MS.gene35375.t1 AT2G36355 62.651 83 31 0 91 173 1 83 1.15e-30 107
MS.gene35375.t1 AT2G36355 61.628 86 33 0 88 173 27 112 1.43e-30 107
MS.gene35375.t1 AT2G36355 44.144 111 55 3 22 130 5 110 3.07e-22 87.0

Find 29 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTCTAACCTCCACTAATT+TGG 0.202427 2.1:-16423115 None:intergenic
AGAAATTGAGAGAAGAAAAT+TGG 0.229998 2.1:+16421754 MS.gene35375:CDS
ATTGCAACATCCTTCCTCTT+TGG 0.283049 2.1:-16422425 None:intergenic
TTTCATCTCTTCTTGGACTC+TGG 0.297684 2.1:-16421679 None:intergenic
GGGTCAAACCTGCCAGAAAA+AGG 0.317850 2.1:+16422493 MS.gene35375:CDS
GTGCAACATGTGAAGAATTC+AGG 0.351410 2.1:+16421641 MS.gene35375:CDS
TCTAGCAATGTTTAGAGAAA+AGG 0.359449 2.1:+16421727 MS.gene35375:CDS
TTCTGGCAGGTTTGACCCAA+TGG 0.366095 2.1:-16422488 None:intergenic
AAAATTGGAGGTTAGAGATA+AGG 0.419428 2.1:+16421769 MS.gene35375:CDS
GAGTCCAAGAAGAGATGAAA+AGG 0.433573 2.1:+16421682 MS.gene35375:CDS
TAACAAAGAGTTAAAGCCAT+TGG 0.438266 2.1:+16422472 MS.gene35375:CDS
AACAAAGAGTTAAAGCCATT+GGG 0.451012 2.1:+16422473 MS.gene35375:CDS
AGAAATTGCATAGGAGATTG+AGG 0.462032 2.1:+16422391 MS.gene35375:intron
GAGGATAAGGATGAAGAAGC+TGG 0.468797 2.1:+16423265 MS.gene35375:CDS
TCAACTTGTTACCAAATTAG+TGG 0.474598 2.1:+16423104 MS.gene35375:CDS
GAAATTGCATAGGAGATTGA+GGG 0.490039 2.1:+16422392 MS.gene35375:intron
GATAAGGTTCATGCTCATCT+TGG 0.506717 2.1:+16421785 MS.gene35375:CDS
TCATCTTGGTCGAGTCGAAA+AGG 0.517837 2.1:+16421799 MS.gene35375:CDS
TGACTGAAAGTGAGAGGATA+AGG 0.518680 2.1:+16423252 MS.gene35375:CDS
AAGAAGAGATGAAAAGGAAG+AGG 0.527146 2.1:+16421688 MS.gene35375:CDS
AATTGAGAGAAGAAAATTGG+AGG 0.531288 2.1:+16421757 MS.gene35375:CDS
CGAAAAGGAAACAAAGCGAT+TGG 0.535815 2.1:+16421814 MS.gene35375:CDS
AGAATACAATGAAGCTCTTG+AGG 0.569694 2.1:+16423053 MS.gene35375:CDS
TTTCACAGATCCAAAGAGGA+AGG 0.618763 2.1:+16422415 MS.gene35375:CDS
AGGGTTTCACAGATCCAAAG+AGG 0.622777 2.1:+16422411 MS.gene35375:CDS
AGCTTGTGACTGAAAGTGAG+AGG 0.623994 2.1:+16423246 MS.gene35375:intron
ACTTGTTACCAAATTAGTGG+AGG 0.627774 2.1:+16423107 MS.gene35375:CDS
GATAAGGATGAAGAAGCTGG+AGG 0.694341 2.1:+16423268 MS.gene35375:CDS
AGTTAGAAAGAAGATAGACA+TGG 0.715757 2.1:+16422448 MS.gene35375:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTTTTAAAAACAATGTA+CGG - chr2.1:16422948-16422967 None:intergenic 10.0%
!! ATATGTTTATTTAGTTGTTT+AGG + chr2.1:16422734-16422753 MS.gene35375:intron 15.0%
!! CAAACAAAGATTATTTATAA+TGG - chr2.1:16422371-16422390 None:intergenic 15.0%
!! GAAATTAAAGCAAATAATTA+AGG - chr2.1:16422670-16422689 None:intergenic 15.0%
!! TTATAATATCTTTACAACAT+AGG + chr2.1:16422563-16422582 MS.gene35375:intron 15.0%
!! TTTAATATTGTAGAAAATCA+CGG + chr2.1:16422054-16422073 MS.gene35375:intron 15.0%
!!! AATTATTTGCTTTAATTTCT+TGG + chr2.1:16422671-16422690 MS.gene35375:intron 15.0%
!!! TTGTGAAACTATATATTTTT+GGG + chr2.1:16423018-16423037 MS.gene35375:intron 15.0%
!! AAAAAAGAAATATGTTAGCT+AGG - chr2.1:16423191-16423210 None:intergenic 20.0%
!! ATTAAAGCAAATAATTAAGG+TGG - chr2.1:16422667-16422686 None:intergenic 20.0%
!!! CTTGTGAAACTATATATTTT+TGG + chr2.1:16423017-16423036 MS.gene35375:intron 20.0%
!!! CTTTTGATGACATAATATAT+GGG - chr2.1:16422698-16422717 None:intergenic 20.0%
!!! TGTGAAACTATATATTTTTG+GGG + chr2.1:16423019-16423038 MS.gene35375:intron 20.0%
!!! TTCATTTGTTTGGTTTTTAT+TGG + chr2.1:16421961-16421980 MS.gene35375:intron 20.0%
!!! TTGTTTGTTTGGTTTTTTAT+TGG + chr2.1:16421904-16421923 MS.gene35375:intron 20.0%
! ACAATATAACGTTGAAATCA+CGG + chr2.1:16422127-16422146 MS.gene35375:intron 25.0%
! AGAAATTGAGAGAAGAAAAT+TGG + chr2.1:16421754-16421773 MS.gene35375:CDS 25.0%
! ATATCAAAAGCAAAACACAT+TGG - chr2.1:16422614-16422633 None:intergenic 25.0%
! ATGATGTTTCATTGTTTGTT+TGG + chr2.1:16421893-16421912 MS.gene35375:intron 25.0%
! ATTTCAACGTTATATTGTCA+CGG - chr2.1:16422125-16422144 None:intergenic 25.0%
! CAATGAGAAAAACAAAGAAA+AGG + chr2.1:16423080-16423099 MS.gene35375:CDS 25.0%
! CCATATATTATGTCATCAAA+AGG + chr2.1:16422696-16422715 MS.gene35375:intron 25.0%
! TAAAACTAAAGCTTAATTCC+TGG - chr2.1:16422191-16422210 None:intergenic 25.0%
! TATATTATGTCATCAAAAGG+AGG + chr2.1:16422699-16422718 MS.gene35375:intron 25.0%
! TATCAAAAGCAAAACACATT+GGG - chr2.1:16422613-16422632 None:intergenic 25.0%
! TGTGCAAAGTTTCAAAATAA+TGG + chr2.1:16422004-16422023 MS.gene35375:intron 25.0%
! TGTTCAAAGTTTCATTTGTT+TGG + chr2.1:16421951-16421970 MS.gene35375:intron 25.0%
! TTACATTTGATCTATAGTCA+AGG + chr2.1:16422208-16422227 MS.gene35375:intron 25.0%
!!! CCTTTTGATGACATAATATA+TGG - chr2.1:16422699-16422718 None:intergenic 25.0%
AAAATTGGAGGTTAGAGATA+AGG + chr2.1:16421769-16421788 MS.gene35375:CDS 30.0%
AACAAAGAGTTAAAGCCATT+GGG + chr2.1:16422473-16422492 MS.gene35375:CDS 30.0%
AACAGTTAGTACTAAACAGT+TGG - chr2.1:16422528-16422547 None:intergenic 30.0%
AAGTTTCAAAATAATGGTCG+CGG + chr2.1:16422010-16422029 MS.gene35375:intron 30.0%
AATTGAGAGAAGAAAATTGG+AGG + chr2.1:16421757-16421776 MS.gene35375:CDS 30.0%
AATTTGAATTCCGTCTGTTT+CGG - chr2.1:16422871-16422890 None:intergenic 30.0%
AGTTAGAAAGAAGATAGACA+TGG + chr2.1:16422448-16422467 MS.gene35375:CDS 30.0%
CAAATTCAGTGTTTGTTTAC+TGG - chr2.1:16421860-16421879 None:intergenic 30.0%
CAATATTAAAGATTACTGCG+TGG - chr2.1:16422044-16422063 None:intergenic 30.0%
TAACAAAGAGTTAAAGCCAT+TGG + chr2.1:16422472-16422491 MS.gene35375:CDS 30.0%
TCTAGCAATGTTTAGAGAAA+AGG + chr2.1:16421727-16421746 MS.gene35375:CDS 30.0%
TTAAAAACAATGTACGGAAG+AGG - chr2.1:16422942-16422961 None:intergenic 30.0%
! CTTTGCATTATGTGAACTTT+TGG + chr2.1:16423220-16423239 MS.gene35375:intron 30.0%
! CTTTGTTTGAGAAATTGCAT+AGG + chr2.1:16422382-16422401 MS.gene35375:intron 30.0%
! TACAACATAGGATTTGTTCA+AGG + chr2.1:16422575-16422594 MS.gene35375:intron 30.0%
! TGCTGATCATATGATCTTTT+TGG - chr2.1:16421605-16421624 None:intergenic 30.0%
! TGGAAAATCTAACCTTTTTC+TGG - chr2.1:16422508-16422527 None:intergenic 30.0%
!! TCAACTTGTTACCAAATTAG+TGG + chr2.1:16423104-16423123 MS.gene35375:CDS 30.0%
!!! CTATATATTTTTGGGGTTAC+AGG + chr2.1:16423026-16423045 MS.gene35375:intron 30.0%
!!! CTGGTCAAGGTTTTAAAAAA+CGG - chr2.1:16422172-16422191 None:intergenic 30.0%
AAGAAGAGATGAAAAGGAAG+AGG + chr2.1:16421688-16421707 MS.gene35375:CDS 35.0%
ACTTCTAACCTCCACTAATT+TGG - chr2.1:16423118-16423137 None:intergenic 35.0%
AGAAATTGCATAGGAGATTG+AGG + chr2.1:16422391-16422410 MS.gene35375:intron 35.0%
AGAATACAATGAAGCTCTTG+AGG + chr2.1:16423053-16423072 MS.gene35375:CDS 35.0%
AGACTAAAGTCTAAGCAATG+TGG - chr2.1:16422639-16422658 None:intergenic 35.0%
CGGAATTCAAATTTCTCAGT+TGG + chr2.1:16422878-16422897 MS.gene35375:intron 35.0%
GAAATTGCATAGGAGATTGA+GGG + chr2.1:16422392-16422411 MS.gene35375:intron 35.0%
TAAAGCTTAATTCCTGGTCA+AGG - chr2.1:16422185-16422204 None:intergenic 35.0%
TTGTCACGGATATCAAATTG+TGG - chr2.1:16422111-16422130 None:intergenic 35.0%
! ACTTGTTACCAAATTAGTGG+AGG + chr2.1:16423107-16423126 MS.gene35375:CDS 35.0%
! TCTTCCTTTTCATCTCTTCT+TGG - chr2.1:16421689-16421708 None:intergenic 35.0%
!! AAATCTAACCTTTTTCTGGC+AGG - chr2.1:16422504-16422523 None:intergenic 35.0%
!! AACTAGCTTGTTTCAAACAC+TGG - chr2.1:16422261-16422280 None:intergenic 35.0%
!!! CGTTTTTTAAAACCTTGACC+AGG + chr2.1:16422170-16422189 MS.gene35375:intron 35.0%
ATTGCAACATCCTTCCTCTT+TGG - chr2.1:16422428-16422447 None:intergenic 40.0%
CGAAAAGGAAACAAAGCGAT+TGG + chr2.1:16421814-16421833 MS.gene35375:CDS 40.0%
GACTTTGTAACCGAAACAGA+CGG + chr2.1:16422858-16422877 MS.gene35375:intron 40.0%
GAGTCCAAGAAGAGATGAAA+AGG + chr2.1:16421682-16421701 MS.gene35375:CDS 40.0%
GATAAGGTTCATGCTCATCT+TGG + chr2.1:16421785-16421804 MS.gene35375:CDS 40.0%
GTCAATCTCTTACCACCAAA+CGG - chr2.1:16422841-16422860 None:intergenic 40.0%
GTGCAACATGTGAAGAATTC+AGG + chr2.1:16421641-16421660 MS.gene35375:CDS 40.0%
TCAAATTGTGGTCGCTATTG+CGG - chr2.1:16422099-16422118 None:intergenic 40.0%
TGACTGAAAGTGAGAGGATA+AGG + chr2.1:16423252-16423271 MS.gene35375:CDS 40.0%
TTTCACAGATCCAAAGAGGA+AGG + chr2.1:16422415-16422434 MS.gene35375:CDS 40.0%
TTTCATCTCTTCTTGGACTC+TGG - chr2.1:16421682-16421701 None:intergenic 40.0%
! AAATAATGGTCGCGGTTTTG+CGG + chr2.1:16422018-16422037 MS.gene35375:intron 40.0%
AGCTTGTGACTGAAAGTGAG+AGG + chr2.1:16423246-16423265 MS.gene35375:intron 45.0%
AGGGTTTCACAGATCCAAAG+AGG + chr2.1:16422411-16422430 MS.gene35375:CDS 45.0%
ATCTCTTACCACCAAACGGA+AGG - chr2.1:16422837-16422856 None:intergenic 45.0%
GAGGATAAGGATGAAGAAGC+TGG + chr2.1:16423265-16423284 MS.gene35375:CDS 45.0%
GATAAGGATGAAGAAGCTGG+AGG + chr2.1:16423268-16423287 MS.gene35375:CDS 45.0%
TCATCTTGGTCGAGTCGAAA+AGG + chr2.1:16421799-16421818 MS.gene35375:CDS 45.0%
TGCTTCTTGTTCCTTCCGTT+TGG + chr2.1:16422823-16422842 MS.gene35375:intron 45.0%
TTCTTGTTCCTTCCGTTTGG+TGG + chr2.1:16422826-16422845 MS.gene35375:intron 45.0%
!! AAATAAAATAAAATAAAATG+TGG + chr2.1:16422294-16422313 MS.gene35375:intron 5.0%
GGGTCAAACCTGCCAGAAAA+AGG + chr2.1:16422493-16422512 MS.gene35375:CDS 50.0%
TTCTGGCAGGTTTGACCCAA+TGG - chr2.1:16422491-16422510 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 16421593 16423322 16421593 ID=MS.gene35375
chr2.1 mRNA 16421593 16423322 16421593 ID=MS.gene35375.t1;Parent=MS.gene35375
chr2.1 exon 16421593 16421850 16421593 ID=MS.gene35375.t1.exon1;Parent=MS.gene35375.t1
chr2.1 CDS 16421593 16421850 16421593 ID=cds.MS.gene35375.t1;Parent=MS.gene35375.t1
chr2.1 exon 16422404 16422514 16422404 ID=MS.gene35375.t1.exon2;Parent=MS.gene35375.t1
chr2.1 CDS 16422404 16422514 16422404 ID=cds.MS.gene35375.t1;Parent=MS.gene35375.t1
chr2.1 exon 16423048 16423128 16423048 ID=MS.gene35375.t1.exon3;Parent=MS.gene35375.t1
chr2.1 CDS 16423048 16423128 16423048 ID=cds.MS.gene35375.t1;Parent=MS.gene35375.t1
chr2.1 exon 16423248 16423322 16423248 ID=MS.gene35375.t1.exon4;Parent=MS.gene35375.t1
chr2.1 CDS 16423248 16423322 16423248 ID=cds.MS.gene35375.t1;Parent=MS.gene35375.t1
Gene Sequence

>MS.gene35375

ATGGCAACACCAAAAAGATCATATGATCAGCAACAACAACTACAGATTGTGCAACATGTGAAGAATTCAGGAATGATTAACAACATCCAGAGTCCAAGAAGAGATGAAAAGGAAGAGGAAATGTCAAGATCAGTTCTAGCAATGTTTAGAGAAAAGGAAGAAGAAATTGAGAGAAGAAAATTGGAGGTTAGAGATAAGGTTCATGCTCATCTTGGTCGAGTCGAAAAGGAAACAAAGCGATTGGCTGAGATAAGAGAAGTAAGTCCAGTAAACAAACACTGAATTTGAGCTAGATTGTTTATGATGTTTCATTGTTTGTTTGGTTTTTTATTGGTTTATTTATGCATTTATAAATCTTTGTTCAAAGTTTCATTTGTTTGGTTTTTATTGGTTTATGCATTTATAAATCTTTGTGCAAAGTTTCAAAATAATGGTCGCGGTTTTGCGGCCACGCAGTAATCTTTAATATTGTAGAAAATCACGGATAAATACAACTTATGTTGCCGCAATAGCGACCACAATTTGATATCCGTGACAATATAACGTTGAAATCACGGCTATATTTTCTGTTGCAGACCGTTTTTTAAAACCTTGACCAGGAATTAAGCTTTAGTTTTACATTTGATCTATAGTCAAGGAAGATTGTATTCACATGTTCATATATACCAGTGTTTGAAACAAGCTAGTTAGAAACAATTTAAAAATAAAATAAAATAAAATGTGGTATTTTTAAAAAAAATTATTCACATTCTGTTATATTCAAGATTTTTTTCATCCATTATAAATAATCTTTGTTTGAGAAATTGCATAGGAGATTGAGGGTTTCACAGATCCAAAGAGGAAGGATGTTGCAATAGTTAGAAAGAAGATAGACATGGTTAACAAAGAGTTAAAGCCATTGGGTCAAACCTGCCAGAAAAAGGTTAGATTTTCCAACTGTTTAGTACTAACTGTTGTTTGCTTTTTTCTTTTATAATATCTTTACAACATAGGATTTGTTCAAGGAGACAAAGTGATCCCAATGTGTTTTGCTTTTGATATAACCACATTGCTTAGACTTTAGTCTATTGACCACCTTAATTATTTGCTTTAATTTCTTGGTCCCATATATTATGTCATCAAAAGGAGGCTATATAATACTATATGTTTATTTAGTTGTTTAGGTGATTATTCAAAATAATTGTGTTTGAATTTGCAAGTAGAAAATTGCTATTTGAAATTCGTCATACATGCTTCTTGTTCCTTCCGTTTGGTGGTAAGAGATTGACTTTGTAACCGAAACAGACGGAATTCAAATTTCTCAGTTGGAAAATGTCATTTTTGCAACTTTAATTTATGATAATTTTCCTCTTCCGTACATTGTTTTTAAAAAATAAATTAAAACAAGAATGAAAGTATGTTAACTATAAAAGTTTCTGAATCTTCTTGTGAAACTATATATTTTTGGGGTTACAGGAGAGAGAATACAATGAAGCTCTTGAGGCTTTCAATGAGAAAAACAAAGAAAAGGCTCAACTTGTTACCAAATTAGTGGAGGTTAGAAGTTATTCATTGATTTAAATGTTAGTACACTAACAATATCATATATTTTTGTTCCTAGCTAACATATTTCTTTTTTATTCTCTCTCTTTGCATTATGTGAACTTTTGGATTAGCTTGTGACTGAAAGTGAGAGGATAAGGATGAAGAAGCTGGAGGATCTAAGCAAAAATATAGATACACTTCACTAA

Protein sequence

>MS.gene35375.t1

MATPKRSYDQQQQLQIVQHVKNSGMINNIQSPRRDEKEEEMSRSVLAMFREKEEEIERRKLEVRDKVHAHLGRVEKETKRLAEIREEIEGFTDPKRKDVAIVRKKIDMVNKELKPLGQTCQKKEREYNEALEAFNEKNKEKAQLVTKLVELVTESERIRMKKLEDLSKNIDTLH