AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene08922


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08922.t1 MTR_1g031680 67.883 137 38 5 1 132 1 136 2.55e-56 172
MS.gene08922.t1 MTR_3g065950 45.926 135 68 3 1 133 4 135 7.59e-36 120
MS.gene08922.t1 MTR_3g065960 46.154 130 67 2 4 133 9 135 3.50e-33 114
MS.gene08922.t1 MTR_5g078750 50.413 121 54 4 17 135 41 157 2.28e-31 110
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08922.t1 AT1G70780 59.286 140 43 5 1 133 2 134 1.13e-46 148
MS.gene08922.t1 AT1G23150 57.664 137 50 5 1 132 1 134 4.43e-39 129
MS.gene08922.t1 AT5G37730 50.588 85 41 1 16 100 16 99 5.39e-25 94.4

Find 37 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTGATCAACATCAATGTTT+TGG 0.229459 4.4:+17380407 MS.gene08922:CDS
CGCTGGACCGATTCGATTTG+TGG 0.324818 4.4:+17380434 MS.gene08922:CDS
GCCATTCCGTTGGTTGTCTC+TGG 0.349243 4.4:-17381167 None:intergenic
GGAATGGGAGCTTGAAGAAT+TGG 0.358621 4.4:+17381225 MS.gene08922:CDS
CATTGGCTCGCCGCAGGAAT+GGG 0.380492 4.4:+17381210 MS.gene08922:CDS
GAGGGAAGACTTCCTATCTT+AGG 0.398437 4.4:+17380516 MS.gene08922:CDS
TTCTCGCATCCTTAGCGCTC+TGG 0.400064 4.4:-17380380 None:intergenic
GCATTGGCTCGCCGCAGGAA+TGG 0.419805 4.4:+17381209 MS.gene08922:CDS
ATCCGCATTGGCCATTCCGT+TGG 0.457689 4.4:-17381177 None:intergenic
GTAACATCCTTTCCTAAGAT+AGG 0.464705 4.4:-17380528 None:intergenic
CGGTCGCATTGGCTCGCCGC+AGG 0.490804 4.4:+17381204 MS.gene08922:CDS
CTGAGTCCACTGGACACCAT+TGG 0.504639 4.4:+17381104 MS.gene08922:CDS
AGCTCCTCCGTGTGATCCAA+TGG 0.509536 4.4:-17381120 None:intergenic
TGCGGATGGAGCGGTCGCAT+TGG 0.509786 4.4:+17381193 MS.gene08922:CDS
TGTCAACACGTTGGATCTGA+TGG 0.540465 4.4:+17380567 MS.gene08922:CDS
TGCGACCGCTCCATCCGCAT+TGG 0.541505 4.4:-17381189 None:intergenic
AACGGAATGGCCAATGCGGA+TGG 0.542673 4.4:+17381179 MS.gene08922:CDS
AAGTCTTCCCTCACGAGCAT+AGG 0.565718 4.4:-17380505 None:intergenic
CGAGGGAGACCTTGTTGCCA+CGG 0.566086 4.4:+17380461 MS.gene08922:CDS
TCGCTAACCACAAATCGAAT+CGG 0.569838 4.4:-17380441 None:intergenic
GCCAGAGACAACCAACGGAA+TGG 0.577142 4.4:+17381166 MS.gene08922:CDS
CTGGACACCATTGGATCACA+CGG 0.585651 4.4:+17381113 MS.gene08922:CDS
AAGACTTCCTATCTTAGGAA+AGG 0.603187 4.4:+17380521 MS.gene08922:CDS
CTCAAATCCTATGCTCGTGA+GGG 0.605690 4.4:+17380498 MS.gene08922:CDS
TCTCAAATCCTATGCTCGTG+AGG 0.606550 4.4:+17380497 MS.gene08922:CDS
GCTCTTTACTGTCAACACGT+TGG 0.610089 4.4:+17380558 MS.gene08922:CDS
GAAGAAGAACCAGAGCGCTA+AGG 0.610725 4.4:+17380371 MS.gene08922:CDS
ATTCGATTTGTGGTTAGCGA+GGG 0.615327 4.4:+17380444 MS.gene08922:CDS
GATTCGATTTGTGGTTAGCG+AGG 0.620699 4.4:+17380443 MS.gene08922:CDS
TATCAATAACCGTGGCAACA+AGG 0.624010 4.4:-17380470 None:intergenic
AATGGCCAATGCGGATGGAG+CGG 0.628022 4.4:+17381184 MS.gene08922:CDS
AACCAACGGAATGGCCAATG+CGG 0.659288 4.4:+17381175 MS.gene08922:CDS
TGTGATCCAATGGTGTCCAG+TGG 0.669646 4.4:-17381110 None:intergenic
TCTTCAAGCTCCCATTCCTG+CGG 0.695043 4.4:-17381220 None:intergenic
GAGAGCAGTATCAATAACCG+TGG 0.708477 4.4:-17380478 None:intergenic
AAGAAGCCAGAGACAACCAA+CGG 0.709491 4.4:+17381161 MS.gene08922:CDS
GACACCATTGGATCACACGG+AGG 0.754267 4.4:+17381116 MS.gene08922:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATCTATTTATGTTTATG+AGG + chr4.4:17380950-17380969 MS.gene08922:intron 15.0%
!! TTACATTATGATGTTAAATT+GGG + chr4.4:17381012-17381031 MS.gene08922:intron 15.0%
!! AAAAATACGTTTGAATTCAA+TGG - chr4.4:17380905-17380924 None:intergenic 20.0%
!! ATAATATTGTAACCTCAATT+AGG + chr4.4:17380704-17380723 MS.gene08922:intron 20.0%
!! CTTACATTATGATGTTAAAT+TGG + chr4.4:17381011-17381030 MS.gene08922:intron 20.0%
!! TAATCAAAACTTTGCATAAA+AGG - chr4.4:17381056-17381075 None:intergenic 20.0%
!!! CAATTTTCATTTTGTTGAAT+TGG + chr4.4:17380625-17380644 MS.gene08922:intron 20.0%
!!! TTCAAACGTATTTTTAGTAA+TGG + chr4.4:17380910-17380929 MS.gene08922:intron 20.0%
! ATTATGATGTTAAATTGGGT+TGG + chr4.4:17381016-17381035 MS.gene08922:intron 25.0%
! ATTCAATAACGAAAACATCA+TGG - chr4.4:17380772-17380791 None:intergenic 25.0%
! TTATGAGGAATTTCATGTTT+CGG + chr4.4:17380965-17380984 MS.gene08922:intron 25.0%
! TTCAATAACGAAAACATCAT+GGG - chr4.4:17380771-17380790 None:intergenic 25.0%
!! GATGTTTTCGTTATTGAATT+GGG + chr4.4:17380773-17380792 MS.gene08922:intron 25.0%
!! GTTCTTTTCATTGTTGAATT+TGG + chr4.4:17380653-17380672 MS.gene08922:intron 25.0%
!! TGATGTTTTCGTTATTGAAT+TGG + chr4.4:17380772-17380791 MS.gene08922:intron 25.0%
!! TTGATCAACATCAATGTTTT+GGG + chr4.4:17380408-17380427 MS.gene08922:CDS 25.0%
!!! GATTAGTGTCTTTTATTTTG+TGG + chr4.4:17381071-17381090 MS.gene08922:intron 25.0%
AACAAAACCAAACCTAATTG+AGG - chr4.4:17380719-17380738 None:intergenic 30.0%
ATTGTAACCTCAATTAGGTT+TGG + chr4.4:17380709-17380728 MS.gene08922:intron 30.0%
GAATTGGGTAGGAAAAAATT+TGG + chr4.4:17380788-17380807 MS.gene08922:intron 30.0%
TATGAGGAATTTCATGTTTC+GGG + chr4.4:17380966-17380985 MS.gene08922:intron 30.0%
! GTTGATCAACATCAATGTTT+TGG + chr4.4:17380407-17380426 MS.gene08922:CDS 30.0%
! TTTATAACCACACTTGCTTT+TGG + chr4.4:17380839-17380858 MS.gene08922:intron 30.0%
! TTTTCGTTATTGAATTGGGT+AGG + chr4.4:17380777-17380796 MS.gene08922:intron 30.0%
AACAAAACCAAAAGCAAGTG+TGG - chr4.4:17380849-17380868 None:intergenic 35.0%
AAGACTTCCTATCTTAGGAA+AGG + chr4.4:17380521-17380540 MS.gene08922:CDS 35.0%
ATGAGGAATTTCATGTTTCG+GGG + chr4.4:17380967-17380986 MS.gene08922:intron 35.0%
GTAACATCCTTTCCTAAGAT+AGG - chr4.4:17380531-17380550 None:intergenic 35.0%
!!! GCTTTTGGTTTTGTTTGTCA+TGG + chr4.4:17380854-17380873 MS.gene08922:intron 35.0%
TATCAATAACCGTGGCAACA+AGG - chr4.4:17380473-17380492 None:intergenic 40.0%
TCGCTAACCACAAATCGAAT+CGG - chr4.4:17380444-17380463 None:intergenic 40.0%
! ATTCGATTTGTGGTTAGCGA+GGG + chr4.4:17380444-17380463 MS.gene08922:CDS 40.0%
AAGAAGCCAGAGACAACCAA+CGG + chr4.4:17381161-17381180 MS.gene08922:CDS 45.0%
CTCAAATCCTATGCTCGTGA+GGG + chr4.4:17380498-17380517 MS.gene08922:CDS 45.0%
GAGAGCAGTATCAATAACCG+TGG - chr4.4:17380481-17380500 None:intergenic 45.0%
GAGGGAAGACTTCCTATCTT+AGG + chr4.4:17380516-17380535 MS.gene08922:CDS 45.0%
GCTCTTTACTGTCAACACGT+TGG + chr4.4:17380558-17380577 MS.gene08922:CDS 45.0%
GGAATGGGAGCTTGAAGAAT+TGG + chr4.4:17381225-17381244 MS.gene08922:CDS 45.0%
TCTCAAATCCTATGCTCGTG+AGG + chr4.4:17380497-17380516 MS.gene08922:CDS 45.0%
TGTCAACACGTTGGATCTGA+TGG + chr4.4:17380567-17380586 MS.gene08922:CDS 45.0%
! GATTCGATTTGTGGTTAGCG+AGG + chr4.4:17380443-17380462 MS.gene08922:CDS 45.0%
!!! ATCAATGTTTTGGGAAGCGC+TGG + chr4.4:17380417-17380436 MS.gene08922:CDS 45.0%
AACCAACGGAATGGCCAATG+CGG + chr4.4:17381175-17381194 MS.gene08922:CDS 50.0%
AAGTCTTCCCTCACGAGCAT+AGG - chr4.4:17380508-17380527 None:intergenic 50.0%
CTGGACACCATTGGATCACA+CGG + chr4.4:17381113-17381132 MS.gene08922:CDS 50.0%
TCTTCAAGCTCCCATTCCTG+CGG - chr4.4:17381223-17381242 None:intergenic 50.0%
TGTGATCCAATGGTGTCCAG+TGG - chr4.4:17381113-17381132 None:intergenic 50.0%
! GAAGAAGAACCAGAGCGCTA+AGG + chr4.4:17380371-17380390 MS.gene08922:CDS 50.0%
!!! TTTTGCAGCTCTGAGTCCAC+TGG + chr4.4:17381094-17381113 MS.gene08922:intron 50.0%
AACGGAATGGCCAATGCGGA+TGG + chr4.4:17381179-17381198 MS.gene08922:CDS 55.0%
AATGGCCAATGCGGATGGAG+CGG + chr4.4:17381184-17381203 MS.gene08922:CDS 55.0%
AGCTCCTCCGTGTGATCCAA+TGG - chr4.4:17381123-17381142 None:intergenic 55.0%
CTGAGTCCACTGGACACCAT+TGG + chr4.4:17381104-17381123 MS.gene08922:CDS 55.0%
GACACCATTGGATCACACGG+AGG + chr4.4:17381116-17381135 MS.gene08922:CDS 55.0%
GCCAGAGACAACCAACGGAA+TGG + chr4.4:17381166-17381185 MS.gene08922:CDS 55.0%
TTCTCGCATCCTTAGCGCTC+TGG - chr4.4:17380383-17380402 None:intergenic 55.0%
! ATCCGCATTGGCCATTCCGT+TGG - chr4.4:17381180-17381199 None:intergenic 55.0%
! GCCATTCCGTTGGTTGTCTC+TGG - chr4.4:17381170-17381189 None:intergenic 55.0%
!! CGCTGGACCGATTCGATTTG+TGG + chr4.4:17380434-17380453 MS.gene08922:CDS 55.0%
CGAGGGAGACCTTGTTGCCA+CGG + chr4.4:17380461-17380480 MS.gene08922:CDS 60.0%
!! CATTGGCTCGCCGCAGGAAT+GGG + chr4.4:17381210-17381229 MS.gene08922:CDS 60.0%
TGCGACCGCTCCATCCGCAT+TGG - chr4.4:17381192-17381211 None:intergenic 65.0%
TGCGGATGGAGCGGTCGCAT+TGG + chr4.4:17381193-17381212 MS.gene08922:CDS 65.0%
!! GCATTGGCTCGCCGCAGGAA+TGG + chr4.4:17381209-17381228 MS.gene08922:CDS 65.0%
!! CGGTCGCATTGGCTCGCCGC+AGG + chr4.4:17381204-17381223 MS.gene08922:CDS 75.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 17380354 17381289 17380354 ID=MS.gene08922
chr4.4 mRNA 17380354 17381289 17380354 ID=MS.gene08922.t1;Parent=MS.gene08922
chr4.4 exon 17380354 17380588 17380354 ID=MS.gene08922.t1.exon1;Parent=MS.gene08922.t1
chr4.4 CDS 17380354 17380588 17380354 ID=cds.MS.gene08922.t1;Parent=MS.gene08922.t1
chr4.4 exon 17381102 17381289 17381102 ID=MS.gene08922.t1.exon2;Parent=MS.gene08922.t1
chr4.4 CDS 17381102 17381289 17381102 ID=cds.MS.gene08922.t1;Parent=MS.gene08922.t1
Gene Sequence

>MS.gene08922

ATGTTGTTGAGTAAGCAGAAGAAGAACCAGAGCGCTAAGGATGCGAGAAAGTTGTTGATCAACATCAATGTTTTGGGAAGCGCTGGACCGATTCGATTTGTGGTTAGCGAGGGAGACCTTGTTGCCACGGTTATTGATACTGCTCTCAAATCCTATGCTCGTGAGGGAAGACTTCCTATCTTAGGAAAGGATGTTACGAGTTTTGCTCTTTACTGTCAACACGTTGGATCTGATGGTAAATTCTTATTCTTATTTTCATAACTTTTTGTTGCAATTTTCATTTTGTTGAATTGGTAATTGTTCTTTTCATTGTTGAATTTGGTAGAGTAAAAGTTTAGTTTTCTATATTGATAATATTGTAACCTCAATTAGGTTTGGTTTTGTTTGTTCATAATGTTGAAGCTTTGTTTGTTACCCATGATGTTTTCGTTATTGAATTGGGTAGGAAAAAATTTGGTTTTTTTTTTATTTAATTTTGATTGAAATTTATAACCACACTTGCTTTTGGTTTTGTTTGTCATGGTTTTTAAGCTTTATCTGTTATGTTACCATTGAATTCAAACGTATTTTTAGTAATGGTATTAGTAATGATATTGAAAATCTATTTATGTTTATGAGGAATTTCATGTTTCGGGGTTGAGAATTTTGTGTTTCAGACTTACATTATGATGTTAAATTGGGTTGGAAGATGTGAGAGTACCTTTTATGCAAAGTTTTGATTAGTGTCTTTTATTTTGTGGTTTTGCAGCTCTGAGTCCACTGGACACCATTGGATCACACGGAGGAGCTAGAAACTTCATGCTATGCAAGAAGCCAGAGACAACCAACGGAATGGCCAATGCGGATGGAGCGGTCGCATTGGCTCGCCGCAGGAATGGGAGCTTGAAGAATTGGTTCAACAAATCTCTAAGTTCAGTTTATGTTCTCATTAGCTAG

Protein sequence

>MS.gene08922.t1

MLLSKQKKNQSAKDARKLLININVLGSAGPIRFVVSEGDLVATVIDTALKSYAREGRLPILGKDVTSFALYCQHVGSDALSPLDTIGSHGGARNFMLCKKPETTNGMANADGAVALARRRNGSLKNWFNKSLSSVYVLIS