AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene062953

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062953.t1 MTR_4g072620 82.609 92 16 0 1 92 1 92 5.01e-56 168
MS.gene062953.t1 MTR_4g072480 82.609 92 16 0 1 92 1 92 6.89e-56 167
MS.gene062953.t1 MTR_4g072730 83.516 91 15 0 1 91 1 91 2.52e-55 166
MS.gene062953.t1 MTR_4g072680 82.609 92 16 0 1 92 1 92 1.25e-54 164
MS.gene062953.t1 MTR_4g072460 80.435 92 18 0 1 92 1 92 4.19e-53 160
MS.gene062953.t1 MTR_4g072570 84.783 92 14 0 1 92 1 92 1.10e-52 159
MS.gene062953.t1 MTR_4g072500 80.435 92 18 0 1 92 1 92 7.04e-52 157
MS.gene062953.t1 MTR_4g072530 80.000 90 18 0 1 90 1 90 6.23e-50 152
MS.gene062953.t1 MTR_4g072660 76.087 92 17 1 1 92 1 87 9.78e-49 149
MS.gene062953.t1 MTR_4g072640 78.889 90 19 0 1 90 1 90 1.56e-48 149
MS.gene062953.t1 MTR_4g072270 73.118 93 24 1 1 92 1 93 3.83e-48 148
MS.gene062953.t1 MTR_3g084150 77.174 92 21 0 1 92 1 92 8.94e-48 147
MS.gene062953.t1 MTR_4g072310 74.194 93 23 1 1 92 1 93 1.34e-47 147
MS.gene062953.t1 MTR_3g084180 76.087 92 22 0 1 92 1 92 9.78e-47 144
MS.gene062953.t1 MTR_8g461410 75.000 92 23 0 1 92 1 92 2.33e-46 143
MS.gene062953.t1 MTR_4g072860 74.194 93 23 1 1 92 1 93 4.84e-46 142
MS.gene062953.t1 MTR_4g072880 70.968 93 26 1 1 92 1 93 3.23e-45 140
MS.gene062953.t1 MTR_4g072230 74.194 93 21 2 1 92 1 91 1.97e-44 139
MS.gene062953.t1 MTR_4g072250 73.118 93 23 2 1 92 1 92 5.20e-44 137
MS.gene062953.t1 MTR_3g084240 70.652 92 27 0 1 92 1 92 9.20e-44 137
MS.gene062953.t1 MTR_5g008360 81.250 80 15 0 13 92 4 83 1.55e-43 136
MS.gene062953.t1 MTR_3g084170 70.652 92 27 0 1 92 1 92 1.90e-43 136
MS.gene062953.t1 MTR_3g110042 75.862 87 21 0 1 87 1 87 8.27e-43 134
MS.gene062953.t1 MTR_4g072240 68.132 91 28 1 1 90 1 91 8.56e-43 134
MS.gene062953.t1 MTR_4g050700 70.968 93 26 1 1 92 1 93 1.14e-42 134
MS.gene062953.t1 MTR_2g012410 70.968 93 26 1 1 92 1 93 1.14e-42 134
MS.gene062953.t1 MTR_5g008370 71.429 91 26 0 1 91 1 91 1.22e-42 135
MS.gene062953.t1 MTR_4g072870 69.231 91 27 1 1 90 1 91 1.91e-42 134
MS.gene062953.t1 MTR_3g084220 68.478 92 29 0 1 92 1 92 3.92e-42 133
MS.gene062953.t1 MTR_3g117630 75.581 86 21 0 7 92 7 92 5.16e-42 132
MS.gene062953.t1 MTR_3g110032 72.043 93 25 1 1 92 1 93 6.20e-42 132
MS.gene062953.t1 MTR_4g072300 65.934 91 30 1 1 90 1 91 1.08e-41 132
MS.gene062953.t1 MTR_4g072220 65.591 93 31 1 1 92 1 93 3.70e-41 130
MS.gene062953.t1 MTR_4g072330 70.000 90 25 1 1 90 1 88 1.11e-40 129
MS.gene062953.t1 MTR_4g072850 70.213 94 26 1 1 92 1 94 1.60e-40 129
MS.gene062953.t1 MTR_4g072370 71.111 90 24 1 1 90 1 88 1.80e-40 129
MS.gene062953.t1 MTR_3g084250 68.478 92 29 0 1 92 1 92 1.95e-40 129
MS.gene062953.t1 MTR_3g084260 68.817 93 28 1 1 92 1 93 1.03e-39 127
MS.gene062953.t1 MTR_4g072580 70.000 90 22 1 1 90 1 85 1.76e-39 126
MS.gene062953.t1 MTR_4g072550 76.712 73 17 0 20 92 31 103 2.39e-39 126
MS.gene062953.t1 MTR_4g072600 71.111 90 21 1 1 90 1 85 2.47e-39 125
MS.gene062953.t1 MTR_4g072490 67.778 90 24 1 1 90 1 85 2.90e-39 125
MS.gene062953.t1 MTR_4g072830 70.787 89 24 1 1 89 1 87 3.18e-39 125
MS.gene062953.t1 MTR_3g110038 65.217 92 32 0 1 92 1 92 5.00e-39 125
MS.gene062953.t1 MTR_4g072630 68.889 90 24 1 1 90 1 86 5.90e-39 124
MS.gene062953.t1 MTR_4g072260 62.366 93 34 1 1 92 1 93 9.63e-39 124
MS.gene062953.t1 MTR_4g072470 70.000 90 22 1 1 90 1 85 1.46e-38 124
MS.gene062953.t1 MTR_3g084210 67.391 92 30 0 1 92 1 92 1.91e-38 124
MS.gene062953.t1 MTR_4g072890 62.637 91 33 1 1 90 1 91 2.28e-38 123
MS.gene062953.t1 MTR_4g072740 67.778 90 24 1 1 90 1 85 2.60e-38 123
MS.gene062953.t1 MTR_4g072465 67.778 90 25 1 1 90 1 86 1.40e-37 121
MS.gene062953.t1 MTR_3g084190 66.304 92 31 0 1 92 1 92 4.64e-37 120
MS.gene062953.t1 MTR_8g461420 67.778 90 29 0 1 90 1 90 7.43e-37 119
MS.gene062953.t1 MTR_4g072940 68.889 90 26 1 3 90 33 122 7.61e-37 120
MS.gene062953.t1 MTR_3g084230 67.778 90 29 0 1 90 1 90 2.51e-36 118
MS.gene062953.t1 MTR_4g072610 67.778 90 23 1 1 90 1 84 3.38e-36 117
MS.gene062953.t1 MTR_3g084160 65.217 92 32 0 1 92 1 92 5.58e-36 117
MS.gene062953.t1 MTR_4g072540 68.889 90 24 1 1 90 1 86 5.84e-36 117
MS.gene062953.t1 MTR_4g072820 68.132 91 22 2 1 90 1 85 2.46e-35 115
MS.gene062953.t1 MTR_4g072590 70.000 90 22 1 1 90 1 85 3.14e-35 115
MS.gene062953.t1 MTR_4g072720 67.778 90 25 1 1 90 1 86 7.86e-35 114
MS.gene062953.t1 MTR_4g072670 68.889 90 24 1 1 90 1 86 8.77e-35 114
MS.gene062953.t1 MTR_3g110045 67.778 90 29 0 3 92 62 151 1.57e-34 115
MS.gene062953.t1 MTR_4g072380 64.835 91 26 2 1 90 1 86 2.44e-34 113
MS.gene062953.t1 MTR_4g072650 65.556 90 25 1 1 90 1 84 2.74e-34 113
MS.gene062953.t1 MTR_4g072510 65.556 90 27 1 1 90 1 86 3.03e-33 110
MS.gene062953.t1 MTR_5g008350 65.823 79 22 1 1 79 1 74 1.31e-32 108
MS.gene062953.t1 MTR_4g072690 68.889 90 23 1 1 90 1 85 2.52e-32 108
MS.gene062953.t1 MTR_4g072750 72.973 74 18 1 1 72 1 74 7.46e-32 107
MS.gene062953.t1 MTR_4g072450 68.889 90 23 1 1 90 1 85 9.29e-32 106
MS.gene062953.t1 MTR_4g072290 60.215 93 22 2 1 92 1 79 2.13e-31 105
MS.gene062953.t1 MTR_4g072980 53.763 93 37 2 1 92 8 95 1.19e-30 104
MS.gene062953.t1 MTR_4g072910 59.770 87 32 2 4 90 17 100 1.55e-30 103
MS.gene062953.t1 MTR_4g072770 72.727 66 18 0 25 90 15 80 4.63e-30 102
MS.gene062953.t1 MTR_3g117640 71.014 69 19 1 24 91 33 101 6.13e-30 102
MS.gene062953.t1 MTR_4g072560 52.577 97 38 2 1 89 1 97 9.39e-30 102
MS.gene062953.t1 MTR_3g084200 65.278 72 25 0 1 72 1 72 1.05e-29 102
MS.gene062953.t1 MTR_3g110035 64.179 67 24 0 26 92 72 138 6.65e-27 95.9
MS.gene062953.t1 MTR_4g072280 60.000 75 29 1 1 74 1 75 9.85e-26 91.3
MS.gene062953.t1 MTR_6g077850 55.224 67 30 0 24 90 33 99 8.82e-25 89.4
MS.gene062953.t1 MTR_3g117610 58.209 67 27 1 23 89 43 108 7.39e-24 87.4
MS.gene062953.t1 MTR_4g072190 50.588 85 40 2 5 89 17 99 8.38e-24 87.0
MS.gene062953.t1 MTR_3g113310 55.224 67 29 1 23 89 40 105 1.09e-23 87.0
MS.gene062953.t1 MTR_8g076040 56.061 66 28 1 24 89 40 104 1.57e-21 81.3
MS.gene062953.t1 MTR_4g072930 50.617 81 35 2 15 90 30 110 4.34e-21 80.5
MS.gene062953.t1 MTR_5g021820 54.545 66 29 1 24 89 39 103 7.30e-21 79.7
MS.gene062953.t1 MTR_8g461400 45.652 92 24 1 1 92 1 66 8.75e-20 75.9
MS.gene062953.t1 MTR_4g124750 43.182 88 47 2 4 89 46 132 5.14e-19 76.3
MS.gene062953.t1 MTR_2g043960 49.333 75 37 1 15 89 66 139 8.05e-19 76.3
MS.gene062953.t1 MTR_4g072840 46.154 91 17 3 1 90 1 60 2.49e-17 69.3
MS.gene062953.t1 MTR_8g461210 43.023 86 48 1 4 89 14 98 2.47e-16 68.6
MS.gene062953.t1 MTR_7g118010 48.529 68 32 2 25 90 23 89 4.79e-16 67.4
MS.gene062953.t1 MTR_3g092220 47.143 70 34 2 16 83 24 92 5.02e-16 67.8
MS.gene062953.t1 MTR_1g094750 43.529 85 43 3 5 85 26 109 5.94e-16 67.4
MS.gene062953.t1 MTR_1g024085 46.988 83 40 2 4 83 13 94 7.91e-16 68.2
MS.gene062953.t1 MTR_8g026730 47.059 68 33 2 25 90 27 93 2.26e-15 65.9
MS.gene062953.t1 MTR_4g005310 50.000 66 32 1 21 85 58 123 2.47e-15 67.0
MS.gene062953.t1 MTR_1g009150 51.471 68 32 1 18 85 64 130 5.21e-15 66.6
MS.gene062953.t1 MTR_0388s0020 40.000 85 47 2 8 89 58 141 1.69e-14 64.7
MS.gene062953.t1 MTR_4g072160 45.679 81 38 3 6 85 21 96 2.85e-14 63.9
MS.gene062953.t1 MTR_4g005320 50.820 61 29 1 25 85 70 129 9.40e-14 63.2
MS.gene062953.t1 MTR_1g061570 47.059 68 35 1 24 91 18 84 9.73e-14 62.0
MS.gene062953.t1 MTR_1g063950 41.096 73 42 1 16 88 39 110 1.42e-13 61.6
MS.gene062953.t1 MTR_3g109160 42.500 80 45 1 12 91 56 134 1.65e-13 62.4
MS.gene062953.t1 MTR_1g063310 38.636 88 51 2 5 90 6 92 1.91e-13 60.8
MS.gene062953.t1 MTR_7g104940 38.667 75 41 2 15 89 44 113 3.12e-13 60.8
MS.gene062953.t1 MTR_1g059770 43.284 67 34 2 28 91 52 117 3.79e-13 60.8
MS.gene062953.t1 MTR_8g101520 40.909 88 47 2 7 90 28 114 3.86e-13 61.2
MS.gene062953.t1 MTR_7g104950 35.526 76 44 2 14 89 43 113 4.90e-13 60.5
MS.gene062953.t1 MTR_7g101740 46.875 64 33 1 24 87 39 101 9.75e-13 59.3
MS.gene062953.t1 MTR_3g097120 43.243 74 33 2 20 85 4 76 1.02e-12 58.9
MS.gene062953.t1 MTR_4g025830 42.424 66 34 2 25 87 79 143 1.23e-12 60.1
MS.gene062953.t1 MTR_8g101530 38.667 75 45 1 13 87 28 101 2.77e-12 58.9
MS.gene062953.t1 MTR_2g095430 40.000 80 40 3 5 77 22 100 3.25e-12 58.2
MS.gene062953.t1 MTR_1g063290 43.750 64 33 2 24 85 38 100 8.97e-12 56.6
MS.gene062953.t1 MTR_1g063300 43.750 64 33 2 24 85 38 100 1.44e-11 56.2
MS.gene062953.t1 MTR_3g105900 72.222 36 10 0 24 59 1171 1206 1.56e-11 58.9
MS.gene062953.t1 MTR_7g101890 37.975 79 46 2 9 85 36 113 1.57e-11 56.2
MS.gene062953.t1 MTR_2g066830 35.802 81 51 1 10 90 43 122 1.66e-11 56.6
MS.gene062953.t1 MTR_1g063330 35.955 89 51 3 4 87 8 95 1.67e-11 55.8
MS.gene062953.t1 MTR_4g021810 51.613 62 28 2 25 85 26 86 1.73e-11 55.8
MS.gene062953.t1 MTR_8g094980 39.474 76 32 2 23 85 1 75 2.53e-11 55.5
MS.gene062953.t1 MTR_4g057810 33.333 84 53 2 4 85 17 99 3.34e-11 55.5
MS.gene062953.t1 MTR_3g098970 32.051 78 52 1 15 92 43 119 5.08e-11 55.5
MS.gene062953.t1 MTR_8g096470 44.828 58 31 1 28 85 49 105 7.54e-11 54.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062953.t1 AT4G34770 63.333 90 31 1 4 91 14 103 1.13e-34 114
MS.gene062953.t1 AT4G34800 61.176 85 26 2 1 82 1 81 1.16e-31 106
MS.gene062953.t1 AT5G18060 54.945 91 39 2 1 90 1 90 3.07e-31 105
MS.gene062953.t1 AT5G18020 55.682 88 37 2 1 87 1 87 4.96e-31 105
MS.gene062953.t1 AT4G38840 61.628 86 32 1 6 90 14 99 7.84e-31 104
MS.gene062953.t1 AT5G18080 54.444 90 39 2 1 89 1 89 2.02e-30 103
MS.gene062953.t1 AT2G21200 57.143 84 34 2 8 89 3 86 2.15e-30 103
MS.gene062953.t1 AT2G21210 51.579 95 41 2 1 90 1 95 4.16e-30 102
MS.gene062953.t1 AT5G18050 68.750 64 19 1 27 89 26 89 6.98e-30 102
MS.gene062953.t1 AT2G21210 51.579 95 41 2 1 90 68 162 1.68e-29 103
MS.gene062953.t1 AT5G18030 55.294 85 35 2 8 89 3 87 1.69e-29 101
MS.gene062953.t1 AT4G38825 60.000 75 29 1 17 90 15 89 3.01e-29 100
MS.gene062953.t1 AT5G18010 51.648 91 42 2 1 90 1 90 2.22e-28 98.2
MS.gene062953.t1 AT4G38850 56.667 90 32 3 1 87 1 86 1.13e-27 96.7
MS.gene062953.t1 AT3G03840 60.274 73 27 1 21 91 23 95 1.59e-27 96.3
MS.gene062953.t1 AT4G34810 70.968 62 14 1 25 82 48 109 2.31e-27 96.7
MS.gene062953.t1 AT3G03820 52.809 89 36 2 6 91 11 96 3.68e-27 95.5
MS.gene062953.t1 AT3G03850 67.164 67 20 1 27 91 27 93 4.17e-27 95.1
MS.gene062953.t1 AT4G13790 46.067 89 46 1 1 87 1 89 1.29e-25 91.3
MS.gene062953.t1 AT3G03830 59.155 71 26 1 20 87 19 89 2.74e-25 90.5
MS.gene062953.t1 AT4G34780 61.429 70 24 1 23 89 26 95 7.35e-25 90.1
MS.gene062953.t1 AT4G34790 63.077 65 22 1 25 87 39 103 1.39e-23 86.7
MS.gene062953.t1 AT1G75580 51.250 80 38 1 10 89 29 107 6.84e-23 85.1
MS.gene062953.t1 AT4G34760 56.716 67 28 1 23 89 41 106 9.97e-23 84.7
MS.gene062953.t1 AT4G38860 54.412 68 31 0 23 90 38 105 2.94e-22 83.2
MS.gene062953.t1 AT2G21220 55.224 67 29 1 23 89 38 103 3.88e-22 82.8
MS.gene062953.t1 AT1G19830 56.757 74 30 2 17 89 40 112 8.46e-21 80.1
MS.gene062953.t1 AT3G43120 42.697 89 50 1 3 91 57 144 2.90e-20 79.7
MS.gene062953.t1 AT2G16580 55.385 65 28 1 23 87 42 105 3.62e-20 78.2
MS.gene062953.t1 AT4G36110 53.125 64 29 1 24 87 39 101 1.58e-18 73.9
MS.gene062953.t1 AT5G20810 49.315 73 36 1 17 89 71 142 1.76e-18 75.5
MS.gene062953.t1 AT5G20810 49.315 73 36 1 17 89 71 142 2.30e-18 75.5
MS.gene062953.t1 AT2G18010 53.125 64 29 1 24 87 47 109 3.34e-18 73.2
MS.gene062953.t1 AT3G20220 42.697 89 43 3 4 85 20 107 2.11e-17 71.2
MS.gene062953.t1 AT5G66260 44.944 89 45 2 5 90 11 98 3.17e-17 70.5
MS.gene062953.t1 AT2G46690 39.326 89 50 2 6 91 5 92 4.51e-17 70.5
MS.gene062953.t1 AT3G51200 52.632 57 26 1 26 82 41 96 1.11e-15 66.6
MS.gene062953.t1 AT3G12830 41.379 87 49 2 4 90 30 114 1.16e-15 67.4
MS.gene062953.t1 AT1G75590 43.529 85 42 2 4 83 16 99 7.88e-15 65.5
MS.gene062953.t1 AT3G61900 38.298 94 48 3 6 90 5 97 9.24e-15 65.1
MS.gene062953.t1 AT2G37030 47.692 65 33 1 24 88 48 111 9.66e-15 64.7
MS.gene062953.t1 AT4G00880 49.275 69 32 2 24 90 27 94 3.24e-14 63.2
MS.gene062953.t1 AT5G10990 44.048 84 42 3 4 83 16 98 4.42e-14 63.5
MS.gene062953.t1 AT4G34750 45.312 64 34 1 24 87 36 98 4.45e-14 63.5
MS.gene062953.t1 AT4G34750 45.312 64 34 1 24 87 36 98 4.45e-14 63.5
MS.gene062953.t1 AT1G19840 45.238 84 41 2 4 83 16 98 7.10e-14 63.2
MS.gene062953.t1 AT3G09870 50.000 70 32 2 18 85 38 106 2.55e-13 60.8
MS.gene062953.t1 AT4G31320 45.455 66 35 1 20 85 75 139 6.19e-13 61.2
MS.gene062953.t1 AT5G53590 40.278 72 37 2 24 90 45 115 1.47e-12 59.7
MS.gene062953.t1 AT2G24400 50.820 61 29 1 25 85 64 123 2.89e-12 59.3
MS.gene062953.t1 AT3G60690 42.857 70 36 2 21 87 84 152 3.68e-12 58.9
MS.gene062953.t1 AT2G45210 39.437 71 39 2 20 87 74 143 4.07e-12 58.9
MS.gene062953.t1 AT3G53250 40.000 65 35 1 24 88 51 111 4.53e-12 57.8
MS.gene062953.t1 AT3G53250 40.000 65 35 1 24 88 82 142 6.33e-12 58.2
MS.gene062953.t1 AT1G76190 48.333 60 29 2 26 85 22 79 7.68e-12 57.4
MS.gene062953.t1 AT3G03847 40.244 82 45 4 3 82 48 127 9.45e-12 57.0
MS.gene062953.t1 AT1G56150 43.939 66 36 1 25 90 38 102 9.95e-12 56.6
MS.gene062953.t1 AT4G09530 39.474 76 43 2 19 91 22 97 1.61e-11 55.8
MS.gene062953.t1 AT1G16510 43.077 65 36 1 25 89 54 117 3.77e-11 55.8
MS.gene062953.t1 AT1G20470 46.154 65 33 2 21 85 19 81 5.73e-11 55.5
MS.gene062953.t1 AT1G79130 41.538 65 37 1 25 89 48 111 7.27e-11 55.1
MS.gene062953.t1 AT4G12410 42.188 64 33 2 25 85 76 138 7.46e-11 55.5

Find 30 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCGTTTACAAACTATTCTA+AGG 0.281110 4.2:-35594581 MS.gene062953:CDS
CACATAACTTCTTGCTTGAA+TGG 0.304861 4.2:-35594339 MS.gene062953:CDS
TGGCTGGTTCAAGTATGATA+TGG 0.304862 4.2:+35594458 None:intergenic
AGTCAAGCCGAAGAAGAGTT+TGG 0.400452 4.2:-35594417 MS.gene062953:CDS
TTGAACCAGCCATCGTTTCA+AGG 0.412783 4.2:-35594447 MS.gene062953:CDS
CCCTTAGAATAGTTTGTAAA+CGG 0.415563 4.2:+35594580 None:intergenic
ATCATATCCAAACTCTTCTT+CGG 0.425222 4.2:+35594410 None:intergenic
GGCTGGTTCAAGTATGATAT+GGG 0.431694 4.2:+35594459 None:intergenic
ACATAACTTCTTGCTTGAAT+GGG 0.440303 4.2:-35594338 MS.gene062953:CDS
TATGTGTTGGAAGGCTTCTT+CGG 0.441042 4.2:+35594356 None:intergenic
AAAATCTGTAGAAGTGAGAA+AGG 0.471464 4.2:-35594529 MS.gene062953:CDS
AAATCTGTAGAAGTGAGAAA+GGG 0.501254 4.2:-35594528 MS.gene062953:CDS
GGCTTCTTCGGTGCAAGGAA+TGG 0.529253 4.2:+35594368 None:intergenic
CCGTTTACAAACTATTCTAA+GGG 0.531520 4.2:-35594580 MS.gene062953:CDS
GGGTATGTTGCAGTGTATGT+AGG 0.540211 4.2:-35594507 MS.gene062953:CDS
TGGAAGGCTTCTTCGGTGCA+AGG 0.543329 4.2:+35594363 None:intergenic
AGGAATGGTGAGGCCACCCA+TGG 0.554354 4.2:+35594383 None:intergenic
TCAAGCAAGAAGTTATGTGT+TGG 0.558158 4.2:+35594343 None:intergenic
CTCAGTAAGCCTTGAAACGA+TGG 0.561145 4.2:+35594438 None:intergenic
GTTTGGATATGATCATCCCA+TGG 0.570301 4.2:-35594400 MS.gene062953:CDS
GCAAGAAGTTATGTGTTGGA+AGG 0.573373 4.2:+35594347 None:intergenic
GTAAGCCTTGAAACGATGGC+TGG 0.590778 4.2:+35594442 None:intergenic
GGATATGATCATCCCATGGG+TGG 0.600402 4.2:-35594396 MS.gene062953:CDS
TGTTGCAGTGTATGTAGGAG+AGG 0.621670 4.2:-35594502 MS.gene062953:CDS
ATGTAGGAGAGGAACAGAAG+AGG 0.629514 4.2:-35594491 MS.gene062953:CDS
GGAATGGTGAGGCCACCCAT+GGG 0.636587 4.2:+35594384 None:intergenic
TTTGGATATGATCATCCCAT+GGG 0.641263 4.2:-35594399 MS.gene062953:CDS
AATCTGTAGAAGTGAGAAAG+GGG 0.642438 4.2:-35594527 MS.gene062953:CDS
CTTCGGTGCAAGGAATGGTG+AGG 0.665194 4.2:+35594373 None:intergenic
GCTGGTTCAAGTATGATATG+GGG 0.687425 4.2:+35594460 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAAATCTGTAGAAGTGAGAA+AGG - chr4.2:35594391-35594410 MS.gene062953:CDS 30.0%
AAATCTGTAGAAGTGAGAAA+GGG - chr4.2:35594392-35594411 MS.gene062953:CDS 30.0%
ACATAACTTCTTGCTTGAAT+GGG - chr4.2:35594582-35594601 MS.gene062953:CDS 30.0%
ATCATATCCAAACTCTTCTT+CGG + chr4.2:35594513-35594532 None:intergenic 30.0%
CCGTTTACAAACTATTCTAA+GGG - chr4.2:35594340-35594359 MS.gene062953:CDS 30.0%
TCCGTTTACAAACTATTCTA+AGG - chr4.2:35594339-35594358 MS.gene062953:CDS 30.0%
! CCCTTAGAATAGTTTGTAAA+CGG + chr4.2:35594343-35594362 None:intergenic 30.0%
AATCTGTAGAAGTGAGAAAG+GGG - chr4.2:35594393-35594412 MS.gene062953:CDS 35.0%
CACATAACTTCTTGCTTGAA+TGG - chr4.2:35594581-35594600 MS.gene062953:CDS 35.0%
TCAAGCAAGAAGTTATGTGT+TGG + chr4.2:35594580-35594599 None:intergenic 35.0%
TTTGGATATGATCATCCCAT+GGG - chr4.2:35594521-35594540 MS.gene062953:CDS 35.0%
GCAAGAAGTTATGTGTTGGA+AGG + chr4.2:35594576-35594595 None:intergenic 40.0%
GTTTGGATATGATCATCCCA+TGG - chr4.2:35594520-35594539 MS.gene062953:CDS 40.0%
TATGTGTTGGAAGGCTTCTT+CGG + chr4.2:35594567-35594586 None:intergenic 40.0%
! GCTGGTTCAAGTATGATATG+GGG + chr4.2:35594463-35594482 None:intergenic 40.0%
! GGCTGGTTCAAGTATGATAT+GGG + chr4.2:35594464-35594483 None:intergenic 40.0%
! TGGCTGGTTCAAGTATGATA+TGG + chr4.2:35594465-35594484 None:intergenic 40.0%
AGTCAAGCCGAAGAAGAGTT+TGG - chr4.2:35594503-35594522 MS.gene062953:CDS 45.0%
ATGTAGGAGAGGAACAGAAG+AGG - chr4.2:35594429-35594448 MS.gene062953:CDS 45.0%
CTCAGTAAGCCTTGAAACGA+TGG + chr4.2:35594485-35594504 None:intergenic 45.0%
GGGTATGTTGCAGTGTATGT+AGG - chr4.2:35594413-35594432 MS.gene062953:CDS 45.0%
TGTTGCAGTGTATGTAGGAG+AGG - chr4.2:35594418-35594437 MS.gene062953:CDS 45.0%
TTGAACCAGCCATCGTTTCA+AGG - chr4.2:35594473-35594492 MS.gene062953:CDS 45.0%
GGATATGATCATCCCATGGG+TGG - chr4.2:35594524-35594543 MS.gene062953:CDS 50.0%
GTAAGCCTTGAAACGATGGC+TGG + chr4.2:35594481-35594500 None:intergenic 50.0%
! CTTCGGTGCAAGGAATGGTG+AGG + chr4.2:35594550-35594569 None:intergenic 55.0%
! GGCTTCTTCGGTGCAAGGAA+TGG + chr4.2:35594555-35594574 None:intergenic 55.0%
! TGGAAGGCTTCTTCGGTGCA+AGG + chr4.2:35594560-35594579 None:intergenic 55.0%
AGGAATGGTGAGGCCACCCA+TGG + chr4.2:35594540-35594559 None:intergenic 60.0%
GGAATGGTGAGGCCACCCAT+GGG + chr4.2:35594539-35594558 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 35594332 35594610 35594332 ID=MS.gene062953
chr4.2 mRNA 35594332 35594610 35594332 ID=MS.gene062953.t1;Parent=MS.gene062953
chr4.2 exon 35594332 35594610 35594332 ID=MS.gene062953.t1.exon1;Parent=MS.gene062953.t1
chr4.2 CDS 35594332 35594610 35594332 ID=cds.MS.gene062953.t1;Parent=MS.gene062953.t1
Gene Sequence

>MS.gene062953

ATGGGTTTCCGTTTACAAACTATTCTAAGGGCATCAGTTACTTCTAGACAAGCATCATCAAAATCTGTAGAAGTGAGAAAGGGGTATGTTGCAGTGTATGTAGGAGAGGAACAGAAGAGGTTCGTGATCCCCATATCATACTTGAACCAGCCATCGTTTCAAGGCTTACTGAGTCAAGCCGAAGAAGAGTTTGGATATGATCATCCCATGGGTGGCCTCACCATTCCTTGCACCGAAGAAGCCTTCCAACACATAACTTCTTGCTTGAATGGGCTATAA
Protein sequence

>MS.gene062953.t1

MGFRLQTILRASVTSRQASSKSVEVRKGYVAVYVGEEQKRFVIPISYLNQPSFQGLLSQAEEEFGYDHPMGGLTIPCTEEAFQHITSCLNGL