AlfalfaGEDB | MS.gene062954

M. sativa cultivar XinJiangDaYe / MS.gene062954

KEGG: K14488
GO: GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
PFAM: PF02519.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062954.t1	XP_003607135.1	87	92	12	0	1	92	1	92	1.10E-39	172.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062954.t1	P32295	75.6	90	22	0	1	90	1	90	2.8e-34	145.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062954.t1	G7JM89	87.0	92	12	0	1	92	1	92	8.2e-40	172.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene057816	MS.gene062954	0.900988	4.22E-78	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene062954	MS.gene031200	PPI
MS.gene062954	MS.gene045360	PPI
MS.gene062954	MS.gene014222	PPI
MS.gene062954	MS.gene79888	PPI
MS.gene40326	MS.gene062954	PPI
MS.gene062952	MS.gene062954	PPI
MS.gene062954	MS.gene75676	PPI
MS.gene062954	MS.gene75678	PPI
MS.gene062954	MS.gene031199	PPI
MS.gene062954	MS.gene50687	PPI
MS.gene50676	MS.gene062954	PPI
MS.gene062954	MS.gene014223	PPI
MS.gene23370	MS.gene062954	PPI
MS.gene50766	MS.gene062954	PPI
MS.gene77529	MS.gene062954	PPI
MS.gene014667	MS.gene062954	PPI
MS.gene003594	MS.gene062954	PPI
MS.gene062954	MS.gene75673	PPI
MS.gene062954	MS.gene79885	PPI
MS.gene50665	MS.gene062954	PPI
MS.gene062954	MS.gene50764	PPI
MS.gene062954	MS.gene062963	PPI
MS.gene50763	MS.gene062954	PPI
MS.gene045365	MS.gene062954	PPI
MS.gene062954	MS.gene50675	PPI
MS.gene50659	MS.gene062954	PPI
MS.gene062954	MS.gene014670	PPI
MS.gene062954	MS.gene045368	PPI
MS.gene75316	MS.gene062954	PPI
MS.gene057790	MS.gene062954	PPI
MS.gene003624	MS.gene062954	PPI
MS.gene062954	MS.gene75314	PPI
MS.gene062954	MS.gene01764	PPI
MS.gene031201	MS.gene062954	PPI
MS.gene062954	MS.gene75679	PPI
MS.gene062954	MS.gene68760	PPI
MS.gene062954	MS.gene031198	PPI
MS.gene77523	MS.gene062954	PPI
MS.gene057778	MS.gene062954	PPI
MS.gene062954	MS.gene057797	PPI
MS.gene062954	MS.gene003614	PPI
MS.gene062954	MS.gene77528	PPI
MS.gene062954	MS.gene014671	PPI
MS.gene062954	MS.gene003596	PPI
MS.gene062954	MS.gene003577	PPI
MS.gene062954	MS.gene23371	PPI
MS.gene062954	MS.gene75672	PPI
MS.gene062954	MS.gene75674	PPI
MS.gene79890	MS.gene062954	PPI
MS.gene003615	MS.gene062954	PPI
MS.gene062954	MS.gene50654	PPI
MS.gene062954	MS.gene062974	PPI
MS.gene062973	MS.gene062954	PPI
MS.gene003602	MS.gene062954	PPI
MS.gene047517	MS.gene062954	PPI
MS.gene062954	MS.gene003583	PPI
MS.gene062954	MS.gene062951	PPI
MS.gene062954	MS.gene057801	PPI
MS.gene062954	MS.gene77527	PPI
MS.gene062954	MS.gene77524	PPI
MS.gene062954	MS.gene057795	PPI
MS.gene062954	MS.gene68162	PPI
MS.gene062954	MS.gene045363	PPI
MS.gene062954	MS.gene75675	PPI
MS.gene062984	MS.gene062954	PPI
MS.gene062954	MS.gene79887	PPI
MS.gene40327	MS.gene062954	PPI
MS.gene062954	MS.gene50652	PPI
MS.gene062954	MS.gene68761	PPI
MS.gene062954	MS.gene40325	PPI
MS.gene062954	MS.gene68165	PPI
MS.gene062954	MS.gene50677	PPI
MS.gene062954	MS.gene50684	PPI
MS.gene003587	MS.gene062954	PPI
MS.gene062954	MS.gene045362	PPI
MS.gene062954	MS.gene23372	PPI
MS.gene062954	MS.gene014668	PPI
MS.gene062954	MS.gene79886	PPI
MS.gene003590	MS.gene062954	PPI
MS.gene062943	MS.gene062954	PPI
MS.gene063112	MS.gene062954	PPI
MS.gene062954	MS.gene50765	PPI
MS.gene062954	MS.gene062988	PPI
MS.gene062954	MS.gene003586	PPI
MS.gene003608	MS.gene062954	PPI
MS.gene062954	MS.gene003627	PPI
MS.gene062954	MS.gene79891	PPI
MS.gene062954	MS.gene045367	PPI
MS.gene062954	MS.gene045364	PPI
MS.gene75677	MS.gene062954	PPI
MS.gene003625	MS.gene062954	PPI
MS.gene062954	MS.gene014669	PPI
MS.gene062954	MS.gene057808	PPI
MS.gene062954	MS.gene002380	PPI
MS.gene045361	MS.gene062954	PPI
MS.gene062954	MS.gene77526	PPI
MS.gene062954	MS.gene045366	PPI
MS.gene003591	MS.gene062954	PPI
MS.gene062954	MS.gene68163	PPI
MS.gene062979	MS.gene062954	PPI
MS.gene062954	MS.gene75315	PPI
MS.gene062954	MS.gene057818	PPI
MS.gene062954	MS.gene68164	PPI
MS.gene01358	MS.gene062954	PPI
MS.gene062954	MS.gene68759	PPI
MS.gene062954	MS.gene79889	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062954.t1	MTR_4g072620	86.957	92	12	0	1	92	1	92	1.66e-58	174
MS.gene062954.t1	MTR_4g072480	85.870	92	13	0	1	92	1	92	1.63e-57	172
MS.gene062954.t1	MTR_4g072730	84.615	91	14	0	1	91	1	91	3.60e-56	168
MS.gene062954.t1	MTR_4g072570	85.870	92	13	0	1	92	1	92	1.03e-55	167
MS.gene062954.t1	MTR_4g072500	83.696	92	15	0	1	92	1	92	1.21e-55	167
MS.gene062954.t1	MTR_4g072680	84.783	92	14	0	1	92	1	92	1.79e-55	166
MS.gene062954.t1	MTR_4g072460	82.609	92	16	0	1	92	1	92	8.72e-54	162
MS.gene062954.t1	MTR_4g072530	80.000	90	18	0	1	90	1	90	2.16e-52	159
MS.gene062954.t1	MTR_4g072640	80.000	90	18	0	1	90	1	90	2.23e-51	156
MS.gene062954.t1	MTR_4g072660	80.435	92	13	1	1	92	1	87	8.34e-51	154
MS.gene062954.t1	MTR_4g072310	76.344	93	21	1	1	92	1	93	2.52e-49	151
MS.gene062954.t1	MTR_4g072860	78.495	93	19	1	1	92	1	93	2.78e-49	151
MS.gene062954.t1	MTR_4g072270	73.118	93	24	1	1	92	1	93	2.03e-47	146
MS.gene062954.t1	MTR_3g084150	73.913	92	24	0	1	92	1	92	2.22e-47	146
MS.gene062954.t1	MTR_4g072230	76.344	93	19	2	1	92	1	91	3.13e-47	145
MS.gene062954.t1	MTR_3g084180	72.826	92	25	0	1	92	1	92	2.66e-46	143
MS.gene062954.t1	MTR_8g461410	71.739	92	26	0	1	92	1	92	5.36e-46	142
MS.gene062954.t1	MTR_4g072880	68.817	93	28	1	1	92	1	93	1.09e-45	142
MS.gene062954.t1	MTR_4g072250	75.269	93	21	2	1	92	1	92	1.57e-45	141
MS.gene062954.t1	MTR_5g008360	81.250	80	15	0	13	92	4	83	1.68e-44	139
MS.gene062954.t1	MTR_4g072240	70.330	91	26	1	1	90	1	91	3.29e-44	138
MS.gene062954.t1	MTR_4g072870	70.330	91	26	1	1	90	1	91	1.02e-43	137
MS.gene062954.t1	MTR_3g084250	70.652	92	27	0	1	92	1	92	1.63e-43	136
MS.gene062954.t1	MTR_3g084240	68.478	92	29	0	1	92	1	92	2.24e-43	136
MS.gene062954.t1	MTR_3g117630	75.581	86	21	0	7	92	7	92	3.08e-43	135
MS.gene062954.t1	MTR_3g084170	68.478	92	29	0	1	92	1	92	6.42e-43	135
MS.gene062954.t1	MTR_4g072220	66.667	93	30	1	1	92	1	93	1.85e-42	134
MS.gene062954.t1	MTR_3g110042	73.563	87	23	0	1	87	1	87	2.15e-42	134
MS.gene062954.t1	MTR_4g072300	67.033	91	29	1	1	90	1	91	2.25e-42	133
MS.gene062954.t1	MTR_4g072850	70.213	94	26	1	1	92	1	94	2.25e-42	134
MS.gene062954.t1	MTR_4g072490	71.111	90	21	1	1	90	1	85	8.38e-42	132
MS.gene062954.t1	MTR_4g072600	72.222	90	20	1	1	90	1	85	1.46e-41	131
MS.gene062954.t1	MTR_3g084210	70.652	92	27	0	1	92	1	92	1.53e-41	131
MS.gene062954.t1	MTR_5g008370	82.432	74	13	0	18	91	18	91	1.66e-41	133
MS.gene062954.t1	MTR_4g072330	70.000	90	25	1	1	90	1	88	4.36e-41	130
MS.gene062954.t1	MTR_4g072370	71.111	90	24	1	1	90	1	88	4.41e-41	130
MS.gene062954.t1	MTR_4g050700	67.742	93	29	1	1	92	1	93	6.34e-41	130
MS.gene062954.t1	MTR_2g012410	67.742	93	29	1	1	92	1	93	6.34e-41	130
MS.gene062954.t1	MTR_3g110032	67.742	93	29	1	1	92	1	93	9.62e-41	129
MS.gene062954.t1	MTR_4g072470	73.333	90	19	1	1	90	1	85	1.08e-40	129
MS.gene062954.t1	MTR_3g084220	65.217	92	32	0	1	92	1	92	1.12e-40	129
MS.gene062954.t1	MTR_3g084190	70.652	92	27	0	1	92	1	92	1.21e-40	129
MS.gene062954.t1	MTR_4g072740	70.000	90	22	1	1	90	1	85	1.66e-40	129
MS.gene062954.t1	MTR_4g072890	64.835	91	31	1	1	90	1	91	3.00e-40	128
MS.gene062954.t1	MTR_4g072260	63.441	93	33	1	1	92	1	93	3.67e-40	128
MS.gene062954.t1	MTR_4g072580	68.889	90	23	1	1	90	1	85	1.02e-39	127
MS.gene062954.t1	MTR_8g461420	70.000	90	27	0	1	90	1	90	1.33e-39	126
MS.gene062954.t1	MTR_3g084160	69.565	92	28	0	1	92	1	92	1.56e-39	126
MS.gene062954.t1	MTR_3g084230	70.000	90	27	0	1	90	1	90	4.95e-39	125
MS.gene062954.t1	MTR_3g110038	64.130	92	33	0	1	92	1	92	6.65e-39	125
MS.gene062954.t1	MTR_4g072830	68.539	89	26	1	1	89	1	87	7.00e-39	124
MS.gene062954.t1	MTR_4g072940	77.778	72	16	0	21	92	53	124	1.05e-38	125
MS.gene062954.t1	MTR_3g084260	64.516	93	32	1	1	92	1	93	1.59e-38	124
MS.gene062954.t1	MTR_4g072630	64.444	90	28	1	1	90	1	86	2.10e-38	123
MS.gene062954.t1	MTR_4g072550	75.342	73	18	0	20	92	31	103	6.77e-38	122
MS.gene062954.t1	MTR_3g110045	69.892	93	26	1	2	92	59	151	1.80e-37	123
MS.gene062954.t1	MTR_4g072610	67.778	90	23	1	1	90	1	84	1.93e-37	120
MS.gene062954.t1	MTR_4g072590	71.111	90	21	1	1	90	1	85	3.39e-37	120
MS.gene062954.t1	MTR_4g072540	67.778	90	25	1	1	90	1	86	3.77e-37	120
MS.gene062954.t1	MTR_4g072465	63.333	90	29	1	1	90	1	86	3.77e-37	120
MS.gene062954.t1	MTR_4g072380	68.132	91	23	2	1	90	1	86	1.15e-36	119
MS.gene062954.t1	MTR_4g072720	66.667	90	26	1	1	90	1	86	2.99e-36	118
MS.gene062954.t1	MTR_4g072820	67.033	91	23	2	1	90	1	85	1.55e-35	116
MS.gene062954.t1	MTR_4g072670	66.667	90	26	1	1	90	1	86	4.11e-35	115
MS.gene062954.t1	MTR_4g072650	63.333	90	27	1	1	90	1	84	1.81e-34	113
MS.gene062954.t1	MTR_4g072510	64.444	90	28	1	1	90	1	86	4.96e-34	112
MS.gene062954.t1	MTR_4g072450	70.000	90	22	1	1	90	1	85	6.55e-34	112
MS.gene062954.t1	MTR_5g008350	67.089	79	21	1	1	79	1	74	1.03e-32	108
MS.gene062954.t1	MTR_4g072910	68.000	75	23	1	16	90	27	100	1.70e-32	108
MS.gene062954.t1	MTR_4g072980	58.065	93	33	2	1	92	8	95	3.51e-32	108
MS.gene062954.t1	MTR_4g072750	63.529	85	27	2	1	81	1	85	5.14e-32	107
MS.gene062954.t1	MTR_4g072690	66.667	90	25	1	1	90	1	85	8.23e-32	106
MS.gene062954.t1	MTR_4g072770	61.111	90	25	1	1	90	1	80	2.32e-31	105
MS.gene062954.t1	MTR_4g072560	57.831	83	34	1	8	89	15	97	3.76e-31	105
MS.gene062954.t1	MTR_4g072290	58.065	93	24	2	1	92	1	79	9.04e-31	103
MS.gene062954.t1	MTR_3g117640	68.116	69	21	1	24	91	33	101	1.59e-29	101
MS.gene062954.t1	MTR_3g084200	61.111	72	28	0	1	72	1	72	1.48e-28	99.4
MS.gene062954.t1	MTR_4g072280	60.000	75	29	1	1	74	1	75	4.07e-28	97.4
MS.gene062954.t1	MTR_3g110035	63.636	66	24	0	27	92	73	138	2.74e-26	94.4
MS.gene062954.t1	MTR_6g077850	56.716	67	29	0	24	90	33	99	1.00e-25	92.0
MS.gene062954.t1	MTR_3g113310	58.209	67	27	1	23	89	40	105	2.00e-25	91.3
MS.gene062954.t1	MTR_3g117610	59.701	67	26	1	23	89	43	108	1.23e-24	89.4
MS.gene062954.t1	MTR_4g072190	51.765	85	39	2	5	89	17	99	3.57e-24	87.8
MS.gene062954.t1	MTR_4g072930	51.852	81	34	2	15	90	30	110	9.86e-23	84.7
MS.gene062954.t1	MTR_5g021820	54.545	66	29	1	24	89	39	103	2.62e-21	80.9
MS.gene062954.t1	MTR_8g076040	48.101	79	40	1	11	89	27	104	3.53e-21	80.5
MS.gene062954.t1	MTR_8g461400	45.652	92	24	1	1	92	1	66	1.68e-20	77.8
MS.gene062954.t1	MTR_4g124750	51.515	66	31	1	24	89	68	132	2.26e-19	77.4
MS.gene062954.t1	MTR_2g043960	51.515	66	31	1	24	89	75	139	3.33e-19	77.0
MS.gene062954.t1	MTR_4g072840	49.451	91	14	3	1	90	1	60	3.73e-19	73.9
MS.gene062954.t1	MTR_8g461210	43.023	86	48	1	4	89	14	98	5.52e-18	72.8
MS.gene062954.t1	MTR_7g118010	48.529	68	32	2	25	90	23	89	3.97e-17	70.1
MS.gene062954.t1	MTR_3g092220	41.176	85	44	3	1	83	12	92	8.01e-17	69.7
MS.gene062954.t1	MTR_4g005310	48.052	77	34	2	15	85	47	123	1.04e-16	70.5
MS.gene062954.t1	MTR_1g024085	44.578	83	42	2	4	83	13	94	1.59e-16	69.7
MS.gene062954.t1	MTR_0388s0020	40.000	85	47	2	8	89	58	141	2.15e-16	69.7
MS.gene062954.t1	MTR_8g026730	45.588	68	34	2	25	90	27	93	3.85e-16	67.8
MS.gene062954.t1	MTR_1g061570	47.059	68	35	1	24	91	18	84	1.15e-15	67.0
MS.gene062954.t1	MTR_1g009150	50.000	68	33	1	18	85	64	130	2.13e-15	67.8
MS.gene062954.t1	MTR_1g059770	45.714	70	34	2	25	91	49	117	2.46e-15	66.2
MS.gene062954.t1	MTR_4g072160	47.887	71	34	2	16	85	28	96	2.79e-15	66.2
MS.gene062954.t1	MTR_4g025830	43.939	66	33	2	25	87	79	143	2.87e-15	67.0
MS.gene062954.t1	MTR_1g063950	43.056	72	40	1	17	88	40	110	5.31e-15	65.5
MS.gene062954.t1	MTR_1g094750	50.000	62	30	1	24	85	49	109	6.81e-15	64.7
MS.gene062954.t1	MTR_3g109160	43.750	80	44	1	12	91	56	134	1.50e-14	65.1
MS.gene062954.t1	MTR_7g101740	48.438	64	32	1	24	87	39	101	3.68e-14	62.8
MS.gene062954.t1	MTR_4g005320	49.180	61	30	1	25	85	70	129	5.88e-14	63.5
MS.gene062954.t1	MTR_2g066830	39.744	78	46	1	13	90	46	122	2.03e-13	61.6
MS.gene062954.t1	MTR_3g097120	44.595	74	32	2	20	85	4	76	9.15e-13	59.3
MS.gene062954.t1	MTR_8g101520	39.024	82	49	1	9	90	34	114	1.13e-12	60.1
MS.gene062954.t1	MTR_7g104940	37.333	75	42	2	15	89	44	113	1.35e-12	59.3
MS.gene062954.t1	MTR_7g405740	45.902	61	32	1	25	85	67	126	2.10e-12	59.3
MS.gene062954.t1	MTR_8g096470	46.667	60	31	1	26	85	47	105	2.31e-12	58.2
MS.gene062954.t1	MTR_7g104950	35.000	80	46	2	10	89	40	113	2.57e-12	58.5
MS.gene062954.t1	MTR_3g105900	72.222	36	10	0	24	59	1171	1206	2.63e-12	61.2
MS.gene062954.t1	MTR_3g098970	32.051	78	52	1	15	92	43	119	6.64e-12	57.8
MS.gene062954.t1	MTR_8g101530	39.130	69	41	1	19	87	34	101	1.83e-11	56.6
MS.gene062954.t1	MTR_8g096440	39.130	69	41	1	14	82	34	101	3.65e-11	55.8
MS.gene062954.t1	MTR_2g095430	39.506	81	41	3	5	78	22	101	4.70e-11	55.5
MS.gene062954.t1	MTR_8g096500	46.552	58	30	1	25	82	49	105	5.73e-11	55.5
MS.gene062954.t1	MTR_8g094980	40.789	76	31	2	23	85	1	75	9.66e-11	53.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062954.t1	AT4G34770	60.000	90	34	1	4	91	14	103	8.46e-34	112
MS.gene062954.t1	AT3G03840	59.341	91	32	2	3	91	8	95	9.75e-33	109
MS.gene062954.t1	AT5G18020	65.432	81	21	2	8	87	13	87	5.48e-32	107
MS.gene062954.t1	AT4G38840	55.556	99	35	3	1	90	1	99	1.12e-31	107
MS.gene062954.t1	AT2G21200	57.647	85	34	2	7	89	2	86	1.44e-31	106
MS.gene062954.t1	AT5G18060	56.044	91	38	2	1	90	1	90	1.61e-31	106
MS.gene062954.t1	AT5G18080	55.556	90	38	2	1	89	1	89	4.61e-31	105
MS.gene062954.t1	AT4G38850	58.889	90	30	3	1	87	1	86	5.46e-31	105
MS.gene062954.t1	AT3G03850	59.341	91	30	2	3	91	8	93	8.59e-31	104
MS.gene062954.t1	AT4G38825	59.036	83	33	1	9	90	7	89	1.08e-30	104
MS.gene062954.t1	AT3G03820	57.471	87	31	2	8	91	13	96	1.60e-30	103
MS.gene062954.t1	AT5G18050	69.697	66	19	1	25	89	24	89	1.81e-30	103
MS.gene062954.t1	AT5G18030	65.278	72	24	1	20	90	17	88	2.11e-30	103
MS.gene062954.t1	AT2G21210	49.474	95	43	2	1	90	1	95	8.48e-30	102
MS.gene062954.t1	AT5G18010	52.747	91	41	2	1	90	1	90	2.28e-29	100
MS.gene062954.t1	AT2G21210	49.474	95	43	2	1	90	68	162	4.42e-29	102
MS.gene062954.t1	AT4G34800	55.294	85	31	2	1	82	1	81	6.99e-29	99.8
MS.gene062954.t1	AT4G34810	67.742	62	16	1	25	82	48	109	3.31e-27	96.3
MS.gene062954.t1	AT3G03830	53.409	88	32	2	3	87	8	89	7.70e-26	92.0
MS.gene062954.t1	AT4G13790	44.944	89	47	1	1	87	1	89	1.37e-25	91.3
MS.gene062954.t1	AT4G34790	61.538	65	23	1	25	87	39	103	1.40e-24	89.4
MS.gene062954.t1	AT4G34780	61.429	70	24	1	23	89	26	95	3.43e-24	88.2
MS.gene062954.t1	AT1G75580	52.500	80	37	1	10	89	29	107	1.06e-23	87.0
MS.gene062954.t1	AT4G38860	55.882	68	30	0	23	90	38	105	2.00e-23	86.3
MS.gene062954.t1	AT4G34760	57.353	68	28	1	23	90	41	107	2.26e-23	86.3
MS.gene062954.t1	AT2G21220	56.716	67	28	1	23	89	38	103	3.02e-23	85.9
MS.gene062954.t1	AT1G19830	56.164	73	30	2	18	89	41	112	3.52e-20	78.6
MS.gene062954.t1	AT3G43120	41.573	89	51	1	3	91	57	144	3.68e-20	79.3
MS.gene062954.t1	AT2G16580	55.385	65	28	1	23	87	42	105	5.36e-20	77.8
MS.gene062954.t1	AT4G36110	54.688	64	28	1	24	87	39	101	2.06e-19	76.3
MS.gene062954.t1	AT5G66260	48.315	89	42	2	5	90	11	98	2.45e-19	75.9
MS.gene062954.t1	AT5G20810	51.515	66	31	1	24	89	78	142	6.81e-19	76.3
MS.gene062954.t1	AT5G20810	47.945	73	37	1	17	89	71	142	1.20e-18	76.3
MS.gene062954.t1	AT2G18010	53.125	64	29	1	24	87	47	109	2.18e-18	73.6
MS.gene062954.t1	AT3G20220	50.000	76	32	3	11	85	37	107	6.98e-18	72.4
MS.gene062954.t1	AT2G46690	40.698	86	47	2	9	91	8	92	1.08e-17	72.0
MS.gene062954.t1	AT1G75590	50.685	73	31	2	15	83	28	99	1.39e-16	70.1
MS.gene062954.t1	AT5G10990	51.667	60	28	1	24	83	40	98	3.21e-16	68.9
MS.gene062954.t1	AT4G34750	45.312	64	34	1	24	87	36	98	4.58e-16	68.6
MS.gene062954.t1	AT4G34750	45.312	64	34	1	24	87	36	98	4.58e-16	68.6
MS.gene062954.t1	AT3G61900	44.286	70	35	2	24	90	29	97	7.16e-16	67.8
MS.gene062954.t1	AT3G51200	53.125	64	28	2	20	82	34	96	8.57e-16	67.0
MS.gene062954.t1	AT3G12830	41.379	87	49	2	4	90	30	114	1.03e-15	67.4
MS.gene062954.t1	AT1G19840	45.238	84	41	2	4	83	16	98	1.20e-15	67.8
MS.gene062954.t1	AT2G37030	47.692	65	33	1	24	88	48	111	2.88e-15	65.9
MS.gene062954.t1	AT4G00880	47.826	69	33	2	24	90	27	94	4.14e-15	65.5
MS.gene062954.t1	AT5G53590	43.056	72	35	2	24	90	45	115	9.24e-15	65.1
MS.gene062954.t1	AT3G60690	45.455	66	32	2	25	87	88	152	2.55e-14	64.7
MS.gene062954.t1	AT2G45210	40.845	71	38	2	20	87	74	143	4.26e-14	63.9
MS.gene062954.t1	AT2G36210	41.333	75	44	0	15	89	44	118	1.08e-13	62.4
MS.gene062954.t1	AT3G03847	49.275	69	30	2	18	82	60	127	1.37e-13	62.0
MS.gene062954.t1	AT1G56150	46.970	66	34	1	25	90	38	102	1.45e-13	61.2
MS.gene062954.t1	AT4G31320	45.455	66	35	1	20	85	75	139	1.59e-13	62.8
MS.gene062954.t1	AT2G24400	50.820	61	29	1	25	85	64	123	4.31e-13	61.6
MS.gene062954.t1	AT1G16510	44.615	65	35	1	25	89	54	117	1.65e-12	59.3
MS.gene062954.t1	AT3G53250	40.000	65	35	1	24	88	51	111	1.79e-12	58.9
MS.gene062954.t1	AT4G12410	43.750	64	32	2	25	85	76	138	1.89e-12	59.7
MS.gene062954.t1	AT3G09870	48.571	70	33	2	18	85	38	106	2.13e-12	58.5
MS.gene062954.t1	AT3G53250	40.000	65	35	1	24	88	82	142	3.07e-12	58.9
MS.gene062954.t1	AT1G79130	44.615	65	35	1	25	89	48	111	5.74e-12	57.8
MS.gene062954.t1	AT4G22620	39.706	68	37	2	21	85	75	141	6.41e-12	58.2
MS.gene062954.t1	AT4G09530	38.158	76	44	2	19	91	22	97	8.69e-12	56.6
MS.gene062954.t1	AT5G03310	45.070	71	32	3	24	88	38	107	1.32e-11	56.2
MS.gene062954.t1	AT1G76190	47.458	59	29	2	27	85	23	79	2.70e-11	55.8

Find 26 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATATAACTTCTTGCTTGAA+TGG	0.302470	4.2:+35592607	MS.gene062954:CDS
GGTTGGTTCAAGTATGAGAT+AGG	0.370602	4.2:-35592487	None:intergenic
CGTTTACATACTATTCTAAA+GGG	0.377068	4.2:+35592367	MS.gene062954:CDS
CCGTTTACATACTATTCTAA+AGG	0.397138	4.2:+35592366	MS.gene062954:CDS
ATATAACTTCTTGCTTGAAT+GGG	0.446395	4.2:+35592608	MS.gene062954:CDS
CCTTTAGAATAGTATGTAAA+CGG	0.479197	4.2:-35592366	None:intergenic
TACACTGCAACATAGCCCTT+TGG	0.504635	4.2:-35592433	None:intergenic
AGTCAATCTGAGGAAGAGTT+CGG	0.507563	4.2:+35592529	MS.gene062954:CDS
ACAAATCTTGAAACAATGGT+TGG	0.515696	4.2:-35592504	None:intergenic
TCAAGCAAGAAGTTATATGT+TGG	0.522465	4.2:-35592603	None:intergenic
CTCAACAAATCTTGAAACAA+TGG	0.526990	4.2:-35592508	None:intergenic
TTCGGATATGATCATCCAAT+GGG	0.535679	4.2:+35592547	MS.gene062954:CDS
AAAATCTGTGGAAGTTCCAA+AGG	0.548759	4.2:+35592417	MS.gene062954:CDS
GGCTATGTTGCAGTGTATGT+TGG	0.553556	4.2:+35592439	MS.gene062954:CDS
TGGAAGACATCTTCGGAGCA+TGG	0.559524	4.2:-35592583	None:intergenic
GACATCTTCGGAGCATGGAA+TGG	0.559799	4.2:-35592578	None:intergenic
GTTCGGATATGATCATCCAA+TGG	0.566860	4.2:+35592546	MS.gene062954:CDS
TATATGTTGGAAGACATCTT+CGG	0.572640	4.2:-35592590	None:intergenic
GTTGGTTCAAGTATGAGATA+GGG	0.585637	4.2:-35592486	None:intergenic
GGAATGGTGAGGCCACCCAT+TGG	0.588290	4.2:-35592562	None:intergenic
ACAAGCATCATCAAAATCTG+TGG	0.600941	4.2:+35592405	MS.gene062954:CDS
AGATTTGTTGAGTCAATCTG+AGG	0.607255	4.2:+35592519	MS.gene062954:CDS
AAATCTGTGGAAGTTCCAAA+GGG	0.612114	4.2:+35592418	MS.gene062954:CDS
GGATATGATCATCCAATGGG+TGG	0.661502	4.2:+35592550	MS.gene062954:CDS
CTTCGGAGCATGGAATGGTG+AGG	0.671810	4.2:-35592573	None:intergenic
ATGTTGGAGAGAGACAAAAG+CGG	0.822067	4.2:+35592455	MS.gene062954:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATATAACTTCTTGCTTGAAT+GGG	+	chr4.2:35592608-35592627	MS.gene062954:CDS	25.0%
!	CGTTTACATACTATTCTAAA+GGG	+	chr4.2:35592367-35592386	MS.gene062954:CDS	25.0%
!!!	CCTTTAGAATAGTATGTAAA+CGG	-	chr4.2:35592369-35592388	None:intergenic	25.0%
	ACAAATCTTGAAACAATGGT+TGG	-	chr4.2:35592507-35592526	None:intergenic	30.0%
	CATATAACTTCTTGCTTGAA+TGG	+	chr4.2:35592607-35592626	MS.gene062954:CDS	30.0%
	CCGTTTACATACTATTCTAA+AGG	+	chr4.2:35592366-35592385	MS.gene062954:CDS	30.0%
	CTCAACAAATCTTGAAACAA+TGG	-	chr4.2:35592511-35592530	None:intergenic	30.0%
	TATATGTTGGAAGACATCTT+CGG	-	chr4.2:35592593-35592612	None:intergenic	30.0%
	TCAAGCAAGAAGTTATATGT+TGG	-	chr4.2:35592606-35592625	None:intergenic	30.0%
	AAAATCTGTGGAAGTTCCAA+AGG	+	chr4.2:35592417-35592436	MS.gene062954:CDS	35.0%
	AAATCTGTGGAAGTTCCAAA+GGG	+	chr4.2:35592418-35592437	MS.gene062954:CDS	35.0%
	ACAAGCATCATCAAAATCTG+TGG	+	chr4.2:35592405-35592424	MS.gene062954:CDS	35.0%
	TTCGGATATGATCATCCAAT+GGG	+	chr4.2:35592547-35592566	MS.gene062954:CDS	35.0%
!	GTTGGTTCAAGTATGAGATA+GGG	-	chr4.2:35592489-35592508	None:intergenic	35.0%
!!	AGATTTGTTGAGTCAATCTG+AGG	+	chr4.2:35592519-35592538	MS.gene062954:CDS	35.0%
	AGTCAATCTGAGGAAGAGTT+CGG	+	chr4.2:35592529-35592548	MS.gene062954:CDS	40.0%
	ATGTTGGAGAGAGACAAAAG+CGG	+	chr4.2:35592455-35592474	MS.gene062954:CDS	40.0%
	GTTCGGATATGATCATCCAA+TGG	+	chr4.2:35592546-35592565	MS.gene062954:CDS	40.0%
!	GGTTGGTTCAAGTATGAGAT+AGG	-	chr4.2:35592490-35592509	None:intergenic	40.0%
	GGATATGATCATCCAATGGG+TGG	+	chr4.2:35592550-35592569	MS.gene062954:CDS	45.0%
	GGCTATGTTGCAGTGTATGT+TGG	+	chr4.2:35592439-35592458	MS.gene062954:CDS	45.0%
	TACACTGCAACATAGCCCTT+TGG	-	chr4.2:35592436-35592455	None:intergenic	45.0%
	GACATCTTCGGAGCATGGAA+TGG	-	chr4.2:35592581-35592600	None:intergenic	50.0%
	TGGAAGACATCTTCGGAGCA+TGG	-	chr4.2:35592586-35592605	None:intergenic	50.0%
	CTTCGGAGCATGGAATGGTG+AGG	-	chr4.2:35592576-35592595	None:intergenic	55.0%
	GGAATGGTGAGGCCACCCAT+TGG	-	chr4.2:35592565-35592584	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	35592358	35592636	35592358	ID=MS.gene062954
chr4.2	mRNA	35592358	35592636	35592358	ID=MS.gene062954.t1;Parent=MS.gene062954
chr4.2	exon	35592358	35592636	35592358	ID=MS.gene062954.t1.exon1;Parent=MS.gene062954.t1
chr4.2	CDS	35592358	35592636	35592358	ID=cds.MS.gene062954.t1;Parent=MS.gene062954.t1

Gene Sequence	>MS.gene062954 ATGGGTTTCCGTTTACATACTATTCTAAAGGGTTCAGTAACTGCTAGACAAGCATCATCAAAATCTGTGGAAGTTCCAAAGGGCTATGTTGCAGTGTATGTTGGAGAGAGACAAAAGCGGTTTGTGATCCCTATCTCATACTTGAACCAACCATTGTTTCAAGATTTGTTGAGTCAATCTGAGGAAGAGTTCGGATATGATCATCCAATGGGTGGCCTCACCATTCCATGCTCCGAAGATGTCTTCCAACATATAACTTCTTGCTTGAATGGGCTATGA
Protein sequence	>MS.gene062954.t1 MGFRLHTILKGSVTARQASSKSVEVPKGYVAVYVGERQKRFVIPISYLNQPLFQDLLSQSEEEFGYDHPMGGLTIPCSEDVFQHITSCLNGL