AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene062958


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062958.t1 MTR_4g072470 94.118 85 5 0 1 85 1 85 1.80e-54 163
MS.gene062958.t1 MTR_4g072490 84.706 85 13 0 1 85 1 85 1.43e-50 154
MS.gene062958.t1 MTR_4g072740 84.706 85 13 0 1 85 1 85 8.86e-50 151
MS.gene062958.t1 MTR_4g072465 81.395 86 15 1 1 85 1 86 4.98e-47 145
MS.gene062958.t1 MTR_4g072630 80.233 86 16 1 1 85 1 86 9.72e-47 144
MS.gene062958.t1 MTR_4g072370 79.545 88 15 1 1 85 1 88 3.36e-46 142
MS.gene062958.t1 MTR_4g072580 78.824 85 18 0 1 85 1 85 4.36e-46 142
MS.gene062958.t1 MTR_4g072450 91.765 85 7 0 1 85 1 85 4.95e-46 142
MS.gene062958.t1 MTR_4g072330 77.273 88 17 1 1 85 1 88 1.05e-45 141
MS.gene062958.t1 MTR_4g072690 88.235 85 10 0 1 85 1 85 1.60e-45 141
MS.gene062958.t1 MTR_4g072830 79.310 87 15 1 1 84 1 87 5.70e-45 139
MS.gene062958.t1 MTR_4g072670 81.395 86 15 1 1 85 1 86 8.31e-44 136
MS.gene062958.t1 MTR_4g072600 76.471 85 20 0 1 85 1 85 1.93e-43 135
MS.gene062958.t1 MTR_3g084150 68.889 90 23 1 1 85 1 90 1.92e-42 133
MS.gene062958.t1 MTR_4g072610 75.294 85 20 1 1 85 1 84 3.36e-42 132
MS.gene062958.t1 MTR_4g072540 76.744 86 19 1 1 85 1 86 9.31e-42 131
MS.gene062958.t1 MTR_3g084180 67.778 90 24 1 1 85 1 90 1.25e-41 131
MS.gene062958.t1 MTR_3g084170 70.000 90 22 1 1 85 1 90 1.90e-41 130
MS.gene062958.t1 MTR_4g072510 75.581 86 20 1 1 85 1 86 4.47e-41 130
MS.gene062958.t1 MTR_8g461410 66.667 90 25 1 1 85 1 90 4.84e-41 130
MS.gene062958.t1 MTR_5g008350 82.432 74 13 0 1 74 1 74 6.88e-41 129
MS.gene062958.t1 MTR_4g072720 75.581 86 20 1 1 85 1 86 8.64e-41 129
MS.gene062958.t1 MTR_3g110032 69.231 91 22 1 1 85 1 91 9.31e-41 129
MS.gene062958.t1 MTR_4g072650 72.941 85 22 1 1 85 1 84 1.12e-40 129
MS.gene062958.t1 MTR_3g110038 68.889 90 23 1 1 85 1 90 1.69e-40 128
MS.gene062958.t1 MTR_4g072870 68.132 91 23 1 1 85 1 91 1.77e-40 128
MS.gene062958.t1 MTR_3g084240 68.889 90 23 1 1 85 1 90 2.65e-40 128
MS.gene062958.t1 MTR_4g072590 82.353 85 15 0 1 85 1 85 2.84e-40 127
MS.gene062958.t1 MTR_3g084220 67.778 90 24 1 1 85 1 90 6.03e-40 127
MS.gene062958.t1 MTR_4g072880 65.934 91 25 1 1 85 1 91 1.07e-39 126
MS.gene062958.t1 MTR_4g072310 67.033 91 24 1 1 85 1 91 4.62e-39 125
MS.gene062958.t1 MTR_4g072240 64.835 91 26 1 1 85 1 91 8.15e-39 124
MS.gene062958.t1 MTR_4g072890 64.835 91 26 1 1 85 1 91 1.61e-38 123
MS.gene062958.t1 MTR_4g072300 63.736 91 27 1 1 85 1 91 2.31e-38 123
MS.gene062958.t1 MTR_4g072220 63.736 91 27 1 1 85 1 91 3.29e-38 122
MS.gene062958.t1 MTR_4g050700 65.934 91 25 1 1 85 1 91 3.37e-38 122
MS.gene062958.t1 MTR_2g012410 65.934 91 25 1 1 85 1 91 3.37e-38 122
MS.gene062958.t1 MTR_4g072570 67.778 90 24 1 1 85 1 90 3.42e-38 122
MS.gene062958.t1 MTR_4g072480 65.556 90 26 1 1 85 1 90 6.04e-38 122
MS.gene062958.t1 MTR_4g072260 63.736 91 27 1 1 85 1 91 8.65e-38 122
MS.gene062958.t1 MTR_3g084250 66.667 90 25 1 1 85 1 90 1.05e-37 121
MS.gene062958.t1 MTR_4g072250 68.132 91 22 2 1 85 1 90 1.07e-37 121
MS.gene062958.t1 MTR_4g072860 69.231 91 22 1 1 85 1 91 1.21e-37 121
MS.gene062958.t1 MTR_4g072640 66.667 90 25 1 1 85 1 90 1.30e-37 121
MS.gene062958.t1 MTR_3g084260 67.033 91 24 1 1 85 1 91 1.40e-37 121
MS.gene062958.t1 MTR_4g072620 64.444 90 27 1 1 85 1 90 1.98e-37 120
MS.gene062958.t1 MTR_4g072680 65.556 90 26 1 1 85 1 90 2.90e-37 120
MS.gene062958.t1 MTR_4g072270 64.835 91 26 1 1 85 1 91 3.76e-37 120
MS.gene062958.t1 MTR_4g072380 70.930 86 24 1 1 85 1 86 3.76e-37 120
MS.gene062958.t1 MTR_3g110042 66.292 89 25 1 1 84 1 89 4.50e-37 120
MS.gene062958.t1 MTR_3g117630 69.512 82 25 0 4 85 9 90 8.40e-37 119
MS.gene062958.t1 MTR_4g072820 69.767 86 24 2 1 85 1 85 1.18e-36 119
MS.gene062958.t1 MTR_4g072660 64.706 85 30 0 1 85 1 85 2.20e-36 118
MS.gene062958.t1 MTR_4g072850 63.043 92 27 1 1 85 1 92 2.44e-36 118
MS.gene062958.t1 MTR_4g072500 67.033 91 23 2 1 85 1 90 5.42e-36 117
MS.gene062958.t1 MTR_4g072460 63.333 90 28 1 1 85 1 90 6.98e-36 117
MS.gene062958.t1 MTR_4g072230 65.934 91 23 2 1 85 1 89 8.62e-36 116
MS.gene062958.t1 MTR_4g072730 62.222 90 29 1 1 85 1 90 1.23e-35 116
MS.gene062958.t1 MTR_4g072530 65.934 91 24 2 1 85 1 90 2.51e-35 115
MS.gene062958.t1 MTR_3g084190 62.222 90 29 1 1 85 1 90 6.39e-34 112
MS.gene062958.t1 MTR_3g084210 61.111 90 30 1 1 85 1 90 1.00e-33 111
MS.gene062958.t1 MTR_5g008360 65.385 78 27 0 8 85 4 81 2.22e-33 110
MS.gene062958.t1 MTR_8g461420 61.111 90 30 1 1 85 1 90 3.46e-33 110
MS.gene062958.t1 MTR_3g084160 61.111 90 30 1 1 85 1 90 9.07e-33 109
MS.gene062958.t1 MTR_3g084230 60.000 90 31 1 1 85 1 90 1.45e-32 108
MS.gene062958.t1 MTR_5g008370 64.444 90 27 2 1 85 1 90 2.99e-32 109
MS.gene062958.t1 MTR_4g072940 61.111 90 28 1 3 85 33 122 5.54e-32 108
MS.gene062958.t1 MTR_3g110045 61.364 88 29 1 3 85 62 149 1.88e-31 107
MS.gene062958.t1 MTR_3g084200 66.667 72 19 1 1 67 1 72 2.97e-30 103
MS.gene062958.t1 MTR_4g072980 54.348 92 34 2 1 84 1 92 1.14e-29 101
MS.gene062958.t1 MTR_4g072560 57.317 82 32 2 4 84 18 97 2.32e-29 100
MS.gene062958.t1 MTR_4g072550 58.824 85 34 1 1 85 18 101 1.29e-28 99.0
MS.gene062958.t1 MTR_4g072910 56.250 80 34 1 7 85 21 100 3.05e-28 98.2
MS.gene062958.t1 MTR_4g072770 58.824 85 30 2 1 85 1 80 8.07e-28 96.3
MS.gene062958.t1 MTR_3g117640 62.687 67 24 1 20 85 34 100 3.81e-27 95.5
MS.gene062958.t1 MTR_4g072290 54.118 85 31 1 1 85 1 77 2.09e-25 90.1
MS.gene062958.t1 MTR_3g110035 54.321 81 34 1 5 85 59 136 6.52e-25 90.5
MS.gene062958.t1 MTR_4g072750 63.514 74 18 2 1 66 1 73 5.52e-24 86.7
MS.gene062958.t1 MTR_4g072190 51.190 84 36 2 5 84 17 99 2.55e-22 82.8
MS.gene062958.t1 MTR_3g117610 56.923 65 27 1 18 82 43 106 8.37e-22 82.0
MS.gene062958.t1 MTR_6g077850 53.030 66 31 0 20 85 34 99 1.61e-21 80.9
MS.gene062958.t1 MTR_4g072930 51.282 78 32 2 14 85 33 110 1.64e-21 81.3
MS.gene062958.t1 MTR_3g113310 52.239 67 31 1 18 84 40 105 2.72e-21 80.5
MS.gene062958.t1 MTR_4g072280 53.333 75 29 1 1 69 1 75 3.05e-21 79.7
MS.gene062958.t1 MTR_4g072840 49.412 85 18 2 1 85 1 60 3.50e-19 73.9
MS.gene062958.t1 MTR_5g021820 45.238 84 42 2 4 84 21 103 1.44e-18 73.6
MS.gene062958.t1 MTR_8g461400 45.556 90 18 2 1 85 1 64 3.50e-18 71.6
MS.gene062958.t1 MTR_8g076040 52.381 63 29 1 20 82 41 102 6.38e-18 72.0
MS.gene062958.t1 MTR_2g043960 45.556 90 34 2 2 77 44 132 7.95e-18 73.2
MS.gene062958.t1 MTR_4g124750 45.349 86 32 2 6 77 41 125 4.70e-17 71.2
MS.gene062958.t1 MTR_1g009150 50.725 69 33 1 12 80 63 130 1.51e-16 70.5
MS.gene062958.t1 MTR_4g005320 50.820 61 29 1 20 80 70 129 1.57e-15 67.4
MS.gene062958.t1 MTR_4g005310 50.000 62 30 1 20 80 62 123 1.95e-15 67.0
MS.gene062958.t1 MTR_3g109160 44.156 77 42 1 4 80 53 128 3.16e-15 66.6
MS.gene062958.t1 MTR_3g092220 36.471 85 51 2 3 85 16 99 3.88e-15 65.1
MS.gene062958.t1 MTR_7g118010 44.928 69 35 2 19 85 22 89 5.81e-15 64.3
MS.gene062958.t1 MTR_4g025830 42.647 68 35 2 20 84 79 145 9.48e-14 62.8
MS.gene062958.t1 MTR_3g105900 80.000 35 7 0 20 54 1172 1206 1.67e-13 64.3
MS.gene062958.t1 MTR_0388s0020 41.558 77 41 2 11 84 66 141 1.74e-13 61.6
MS.gene062958.t1 MTR_1g061570 46.575 73 36 2 10 80 7 78 2.20e-13 60.8
MS.gene062958.t1 MTR_1g094750 47.541 61 31 1 20 80 50 109 3.85e-13 60.1
MS.gene062958.t1 MTR_8g026730 40.580 69 38 2 19 85 26 93 5.58e-13 59.3
MS.gene062958.t1 MTR_5g008380 84.375 32 5 0 54 85 246 277 8.36e-13 61.6
MS.gene062958.t1 MTR_7g101740 46.032 63 33 1 20 82 40 101 2.24e-12 58.2
MS.gene062958.t1 MTR_1g024085 42.105 76 38 2 4 79 26 95 2.61e-12 58.5
MS.gene062958.t1 MTR_1g063950 43.077 65 36 1 13 77 41 104 4.82e-12 57.4
MS.gene062958.t1 MTR_4g021810 48.571 70 33 3 13 80 18 86 4.85e-12 57.0
MS.gene062958.t1 MTR_8g461210 44.262 61 33 1 20 80 35 94 4.04e-11 55.1
MS.gene062958.t1 MTR_4g072160 45.902 61 32 1 20 80 37 96 5.55e-11 55.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062958.t1 AT4G38840 71.642 67 18 1 20 85 33 99 6.15e-32 107
MS.gene062958.t1 AT2G21210 56.579 76 31 1 12 85 20 95 2.05e-29 100
MS.gene062958.t1 AT5G18030 60.256 78 30 1 9 85 11 88 2.08e-29 100
MS.gene062958.t1 AT5G18020 60.811 74 28 1 13 85 17 90 2.55e-29 100
MS.gene062958.t1 AT5G18050 60.274 73 28 1 13 84 17 89 4.06e-29 100
MS.gene062958.t1 AT5G18080 60.811 74 28 1 13 85 17 90 4.15e-29 99.8
MS.gene062958.t1 AT5G18060 60.811 74 28 1 13 85 17 90 7.50e-29 99.4
MS.gene062958.t1 AT2G21210 56.579 76 31 1 12 85 87 162 1.08e-28 101
MS.gene062958.t1 AT4G34800 60.494 81 28 2 1 77 1 81 2.46e-28 98.2
MS.gene062958.t1 AT5G18010 59.459 74 29 1 13 85 17 90 4.78e-28 97.1
MS.gene062958.t1 AT4G38825 59.722 72 28 1 15 85 18 89 7.20e-28 96.7
MS.gene062958.t1 AT4G34810 67.143 70 18 2 20 84 48 117 1.14e-27 97.1
MS.gene062958.t1 AT2G21200 58.333 72 29 1 14 84 15 86 7.22e-27 94.0
MS.gene062958.t1 AT4G38850 56.790 81 28 2 8 85 13 89 7.93e-27 94.0
MS.gene062958.t1 AT3G03840 57.895 76 30 1 12 85 19 94 2.13e-26 93.2
MS.gene062958.t1 AT4G34770 57.333 75 30 1 13 85 28 102 2.00e-25 90.9
MS.gene062958.t1 AT3G03830 55.844 77 31 1 12 85 16 92 4.59e-25 89.7
MS.gene062958.t1 AT3G03850 61.194 67 24 1 21 85 26 92 4.16e-24 87.4
MS.gene062958.t1 AT4G13790 44.048 84 45 1 3 84 8 91 4.23e-24 87.4
MS.gene062958.t1 AT3G03820 50.649 77 35 1 12 85 19 95 9.00e-24 86.7
MS.gene062958.t1 AT4G34790 63.333 60 20 1 20 77 39 98 7.18e-22 82.0
MS.gene062958.t1 AT4G34780 57.143 70 27 1 18 84 26 95 2.19e-21 80.9
MS.gene062958.t1 AT1G75580 56.716 67 28 1 18 84 42 107 4.64e-21 80.1
MS.gene062958.t1 AT4G38860 51.471 68 33 0 18 85 38 105 2.23e-20 78.2
MS.gene062958.t1 AT4G34760 52.239 67 31 1 18 84 41 106 8.69e-20 76.6
MS.gene062958.t1 AT2G21220 50.746 67 32 1 18 84 38 103 3.18e-19 75.5
MS.gene062958.t1 AT2G16580 52.308 65 30 1 18 82 42 105 5.45e-19 74.7
MS.gene062958.t1 AT4G36110 47.674 86 36 3 5 82 17 101 3.28e-18 72.8
MS.gene062958.t1 AT1G19830 50.649 77 36 2 9 84 37 112 9.13e-18 72.0
MS.gene062958.t1 AT5G66260 46.988 83 42 2 4 85 17 98 1.53e-17 70.9
MS.gene062958.t1 AT3G20220 53.030 66 29 2 19 83 46 110 1.82e-17 71.2
MS.gene062958.t1 AT3G43120 55.172 58 25 1 20 77 79 135 2.50e-17 72.0
MS.gene062958.t1 AT5G20810 45.349 86 32 2 6 77 51 135 6.42e-17 70.9
MS.gene062958.t1 AT2G18010 50.794 63 30 1 20 82 48 109 9.12e-17 69.3
MS.gene062958.t1 AT5G20810 45.349 86 32 2 6 77 51 135 1.06e-16 70.9
MS.gene062958.t1 AT2G37030 50.000 62 30 1 20 81 49 109 8.36e-14 62.0
MS.gene062958.t1 AT4G31320 45.588 68 36 1 13 80 73 139 1.49e-13 62.8
MS.gene062958.t1 AT2G24400 50.000 62 30 1 19 80 63 123 8.42e-13 60.5
MS.gene062958.t1 AT1G75590 45.000 60 32 1 20 79 42 100 1.05e-11 57.4
MS.gene062958.t1 AT3G61900 38.554 83 47 2 6 85 16 97 1.19e-11 56.6
MS.gene062958.t1 AT2G46690 37.179 78 45 2 11 85 15 91 1.21e-11 56.2
MS.gene062958.t1 AT4G34750 39.683 63 37 1 20 82 37 98 1.34e-11 57.0
MS.gene062958.t1 AT4G34750 39.683 63 37 1 20 82 37 98 1.34e-11 57.0
MS.gene062958.t1 AT4G00880 37.647 85 48 3 3 85 13 94 1.35e-11 56.2
MS.gene062958.t1 AT3G51200 46.575 73 33 3 10 77 25 96 1.44e-11 55.8
MS.gene062958.t1 AT4G12410 41.176 68 36 2 20 84 76 142 1.63e-11 57.0
MS.gene062958.t1 AT3G53250 37.500 72 41 1 10 81 42 109 2.98e-11 55.5
MS.gene062958.t1 AT5G10990 43.333 60 33 1 20 79 41 99 3.92e-11 55.8
MS.gene062958.t1 AT4G22620 34.146 82 50 2 6 84 65 145 4.65e-11 55.8
MS.gene062958.t1 AT1G19840 40.789 76 40 2 4 79 29 99 6.48e-11 55.1

Find 18 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCATCATCTTCAAAATCTA+TGG 0.196842 4.2:-35555276 MS.gene062958:CDS
CACTTTGGCTCAGCAATTCT+TGG 0.198061 4.2:+35555165 None:intergenic
TTTGCTTCAAATACGATATA+GGG 0.268810 4.2:+35555195 None:intergenic
GTTTACCTAGTATCAACAAA+AGG 0.443253 4.2:-35555298 MS.gene062958:CDS
ATTCAAATTGTTCCTCACTT+TGG 0.448469 4.2:+35555150 None:intergenic
AAAATCTATGGGTGTTCCAA+AGG 0.503784 4.2:-35555264 MS.gene062958:CDS
TTTGAATATGATCATCCAAT+GGG 0.507762 4.2:-35555134 MS.gene062958:CDS
TTTACCTAGTATCAACAAAA+GGG 0.513723 4.2:-35555297 MS.gene062958:CDS
AAATCTATGGGTGTTCCAAA+GGG 0.531901 4.2:-35555263 MS.gene062958:CDS
TATACAGCTAGATATCCCTT+TGG 0.546226 4.2:+35555248 None:intergenic
ATTTGAATATGATCATCCAA+TGG 0.547817 4.2:-35555135 MS.gene062958:CDS
GCATCATCTTCAAAATCTAT+GGG 0.555641 4.2:-35555275 MS.gene062958:CDS
GAGAAAACTTCTTCTCTGCA+AGG 0.572757 4.2:+35555098 None:intergenic
GGAATAGTGAGACCACCCAT+TGG 0.603922 4.2:+35555119 None:intergenic
AGAATTGCTGAGCCAAAGTG+AGG 0.630409 4.2:-35555162 MS.gene062958:CDS
GAATATGATCATCCAATGGG+TGG 0.646056 4.2:-35555131 MS.gene062958:CDS
TCTAGCTGTATACGTAGGAG+AGG 0.648096 4.2:-35555237 MS.gene062958:CDS
GGATATCTAGCTGTATACGT+AGG 0.688332 4.2:-35555242 MS.gene062958:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATTTGAATATGATCATCCAA+TGG - chr4.2:35555246-35555265 MS.gene062958:CDS 25.0%
! TTTACCTAGTATCAACAAAA+GGG - chr4.2:35555084-35555103 MS.gene062958:CDS 25.0%
! TTTGAATATGATCATCCAAT+GGG - chr4.2:35555247-35555266 MS.gene062958:CDS 25.0%
! TTTGCTTCAAATACGATATA+GGG + chr4.2:35555189-35555208 None:intergenic 25.0%
!! TTTTGCTTCAAATACGATAT+AGG + chr4.2:35555190-35555209 None:intergenic 25.0%
ATTCAAATTGTTCCTCACTT+TGG + chr4.2:35555234-35555253 None:intergenic 30.0%
GCATCATCTTCAAAATCTAT+GGG - chr4.2:35555106-35555125 MS.gene062958:CDS 30.0%
GTTTACCTAGTATCAACAAA+AGG - chr4.2:35555083-35555102 MS.gene062958:CDS 30.0%
AAAATCTATGGGTGTTCCAA+AGG - chr4.2:35555117-35555136 MS.gene062958:CDS 35.0%
AAATCTATGGGTGTTCCAAA+GGG - chr4.2:35555118-35555137 MS.gene062958:CDS 35.0%
GGCATCATCTTCAAAATCTA+TGG - chr4.2:35555105-35555124 MS.gene062958:CDS 35.0%
TATACAGCTAGATATCCCTT+TGG + chr4.2:35555136-35555155 None:intergenic 35.0%
GAATATGATCATCCAATGGG+TGG - chr4.2:35555250-35555269 MS.gene062958:CDS 40.0%
GAGAAAACTTCTTCTCTGCA+AGG + chr4.2:35555286-35555305 None:intergenic 40.0%
GGATATCTAGCTGTATACGT+AGG - chr4.2:35555139-35555158 MS.gene062958:CDS 40.0%
! GATGCCCTTTTGTTGATACT+AGG + chr4.2:35555091-35555110 None:intergenic 40.0%
AGAATTGCTGAGCCAAAGTG+AGG - chr4.2:35555219-35555238 MS.gene062958:CDS 45.0%
TCTAGCTGTATACGTAGGAG+AGG - chr4.2:35555144-35555163 MS.gene062958:CDS 45.0%
! CACTTTGGCTCAGCAATTCT+TGG + chr4.2:35555219-35555238 None:intergenic 45.0%
GGAATAGTGAGACCACCCAT+TGG + chr4.2:35555265-35555284 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 35555073 35555330 35555073 ID=MS.gene062958
chr4.2 mRNA 35555073 35555330 35555073 ID=MS.gene062958.t1;Parent=MS.gene062958
chr4.2 exon 35555073 35555330 35555073 ID=MS.gene062958.t1.exon1;Parent=MS.gene062958.t1
chr4.2 CDS 35555073 35555330 35555073 ID=cds.MS.gene062958.t1;Parent=MS.gene062958.t1
Gene Sequence

>MS.gene062958

ATGGGTTTTCGTTTACCTAGTATCAACAAAAGGGCATCATCTTCAAAATCTATGGGTGTTCCAAAGGGATATCTAGCTGTATACGTAGGAGAGGAGATGAAGAGATTTGTGATCCCTATATCGTATTTGAAGCAAAAATCATTCCAAGAATTGCTGAGCCAAAGTGAGGAACAATTTGAATATGATCATCCAATGGGTGGTCTCACTATTCCTTGCAGAGAAGAAGTTTTCTCAGACATCACTTCTAGATTAAATTGA

Protein sequence

>MS.gene062958.t1

MGFRLPSINKRASSSKSMGVPKGYLAVYVGEEMKRFVIPISYLKQKSFQELLSQSEEQFEYDHPMGGLTIPCREEVFSDITSRLN