AlfalfaGEDB | MS.gene062965

M. sativa cultivar XinJiangDaYe / MS.gene062965

KEGG: K14488
GO: GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
PFAM: PF02519.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062965.t1	AES89321.1	94.2	86	5	0	1	86	1	86	1.80E-39	171.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062965.t1	P33080	68.9	90	24	1	1	86	1	90	4.6e-31	134.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062965.t1	G7JL46	94.2	86	5	0	1	86	1	86	1.3e-39	171.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene062965	MS.gene01764	PPI
MS.gene062965	MS.gene75679	PPI
MS.gene50676	MS.gene062965	PPI
MS.gene003614	MS.gene062965	PPI
MS.gene057797	MS.gene062965	PPI
MS.gene23370	MS.gene062965	PPI
MS.gene50766	MS.gene062965	PPI
MS.gene003594	MS.gene062965	PPI
MS.gene003596	MS.gene062965	PPI
MS.gene50665	MS.gene062965	PPI
MS.gene79889	MS.gene062965	PPI
MS.gene50677	MS.gene062965	PPI
MS.gene68759	MS.gene062965	PPI
MS.gene003586	MS.gene062965	PPI
MS.gene062988	MS.gene062965	PPI
MS.gene062965	MS.gene50764	PPI
MS.gene062965	MS.gene014671	PPI
MS.gene77528	MS.gene062965	PPI
MS.gene50763	MS.gene062965	PPI
MS.gene062965	MS.gene75672	PPI
MS.gene031200	MS.gene062965	PPI
MS.gene045365	MS.gene062965	PPI
MS.gene062965	MS.gene50654	PPI
MS.gene062965	MS.gene062974	PPI
MS.gene50659	MS.gene062965	PPI
MS.gene062965	MS.gene014670	PPI
MS.gene062965	MS.gene062951	PPI
MS.gene75316	MS.gene062965	PPI
MS.gene062965	MS.gene68162	PPI
MS.gene057790	MS.gene062965	PPI
MS.gene062965	MS.gene79887	PPI
MS.gene003624	MS.gene062965	PPI
MS.gene23371	MS.gene062965	PPI
MS.gene031201	MS.gene062965	PPI
MS.gene031199	MS.gene062965	PPI
MS.gene75314	MS.gene062965	PPI
MS.gene77529	MS.gene062965	PPI
MS.gene68761	MS.gene062965	PPI
MS.gene014667	MS.gene062965	PPI
MS.gene68760	MS.gene062965	PPI
MS.gene75676	MS.gene062965	PPI
MS.gene062965	MS.gene50684	PPI
MS.gene057778	MS.gene062965	PPI
MS.gene75673	MS.gene062965	PPI
MS.gene062965	MS.gene014668	PPI
MS.gene045366	MS.gene062965	PPI
MS.gene062965	MS.gene79886	PPI
MS.gene062965	MS.gene50765	PPI
MS.gene062963	MS.gene062965	PPI
MS.gene031198	MS.gene062965	PPI
MS.gene75674	MS.gene062965	PPI
MS.gene79890	MS.gene062965	PPI
MS.gene003615	MS.gene062965	PPI
MS.gene045364	MS.gene062965	PPI
MS.gene062973	MS.gene062965	PPI
MS.gene003602	MS.gene062965	PPI
MS.gene23372	MS.gene062965	PPI
MS.gene062965	MS.gene79891	PPI
MS.gene062965	MS.gene045367	PPI
MS.gene003627	MS.gene062965	PPI
MS.gene003577	MS.gene062965	PPI
MS.gene062965	MS.gene045363	PPI
MS.gene77524	MS.gene062965	PPI
MS.gene062984	MS.gene062965	PPI
MS.gene057801	MS.gene062965	PPI
MS.gene014223	MS.gene062965	PPI
MS.gene40327	MS.gene062965	PPI
MS.gene062965	MS.gene50652	PPI
MS.gene062965	MS.gene014669	PPI
MS.gene062965	MS.gene057808	PPI
MS.gene062965	MS.gene002380	PPI
MS.gene045362	MS.gene062965	PPI
MS.gene77523	MS.gene062965	PPI
MS.gene003587	MS.gene062965	PPI
MS.gene062965	MS.gene68163	PPI
MS.gene062965	MS.gene75315	PPI
MS.gene003590	MS.gene062965	PPI
MS.gene045368	MS.gene062965	PPI
MS.gene063112	MS.gene062965	PPI
MS.gene062965	MS.gene68164	PPI
MS.gene062965	MS.gene014222	PPI
MS.gene062965	MS.gene79888	PPI
MS.gene77526	MS.gene062965	PPI
MS.gene047517	MS.gene062965	PPI
MS.gene045360	MS.gene062965	PPI
MS.gene057818	MS.gene062965	PPI
MS.gene062965	MS.gene79885	PPI
MS.gene50687	MS.gene062965	PPI
MS.gene75677	MS.gene062965	PPI
MS.gene003625	MS.gene062965	PPI
MS.gene057795	MS.gene062965	PPI
MS.gene68165	MS.gene062965	PPI
MS.gene045361	MS.gene062965	PPI
MS.gene50675	MS.gene062965	PPI
MS.gene003591	MS.gene062965	PPI
MS.gene062979	MS.gene062965	PPI
MS.gene40325	MS.gene062965	PPI
MS.gene062943	MS.gene062965	PPI
MS.gene01358	MS.gene062965	PPI
MS.gene003608	MS.gene062965	PPI
MS.gene75675	MS.gene062965	PPI
MS.gene003583	MS.gene062965	PPI
MS.gene40326	MS.gene062965	PPI
MS.gene062952	MS.gene062965	PPI
MS.gene062965	MS.gene75678	PPI
MS.gene77527	MS.gene062965	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062965.t1	MTR_4g072630	94.186	86	5	0	1	86	1	86	3.30e-58	173
MS.gene062965.t1	MTR_4g072465	88.372	86	10	0	1	86	1	86	1.93e-54	163
MS.gene062965.t1	MTR_4g072490	88.372	86	9	1	1	86	1	85	1.40e-53	161
MS.gene062965.t1	MTR_4g072740	87.209	86	10	1	1	86	1	85	9.99e-53	159
MS.gene062965.t1	MTR_4g072510	94.186	86	5	0	1	86	1	86	3.87e-50	152
MS.gene062965.t1	MTR_4g072670	91.860	86	7	0	1	86	1	86	3.79e-49	150
MS.gene062965.t1	MTR_4g072370	81.818	88	14	1	1	86	1	88	4.47e-49	150
MS.gene062965.t1	MTR_4g072580	83.721	86	13	1	1	86	1	85	4.92e-49	150
MS.gene062965.t1	MTR_4g072540	91.860	86	7	0	1	86	1	86	1.26e-48	149
MS.gene062965.t1	MTR_4g072470	82.558	86	14	1	1	86	1	85	3.10e-48	147
MS.gene062965.t1	MTR_4g072830	81.609	87	14	1	1	85	1	87	4.10e-48	147
MS.gene062965.t1	MTR_4g072720	89.535	86	9	0	1	86	1	86	2.13e-47	145
MS.gene062965.t1	MTR_4g072600	80.233	86	16	1	1	86	1	85	2.25e-46	143
MS.gene062965.t1	MTR_4g072610	79.070	86	16	1	1	86	1	84	4.42e-45	140
MS.gene062965.t1	MTR_4g072650	77.907	86	17	1	1	86	1	84	3.15e-44	137
MS.gene062965.t1	MTR_4g072330	75.281	89	18	2	1	86	1	88	3.44e-44	137
MS.gene062965.t1	MTR_3g084170	72.222	90	21	1	1	86	1	90	5.59e-44	137
MS.gene062965.t1	MTR_4g072870	71.739	92	19	2	1	86	1	91	8.70e-44	137
MS.gene062965.t1	MTR_3g084150	71.111	90	22	1	1	86	1	90	2.66e-43	135
MS.gene062965.t1	MTR_3g084240	71.111	90	22	1	1	86	1	90	6.32e-43	134
MS.gene062965.t1	MTR_5g008350	82.667	75	12	1	1	75	1	74	1.12e-42	133
MS.gene062965.t1	MTR_3g084220	70.000	90	23	1	1	86	1	90	1.49e-42	134
MS.gene062965.t1	MTR_3g084180	70.000	90	23	1	1	86	1	90	2.16e-42	133
MS.gene062965.t1	MTR_4g072690	80.233	86	16	1	1	86	1	85	3.67e-42	132
MS.gene062965.t1	MTR_3g110038	70.000	90	23	1	1	86	1	90	4.08e-42	132
MS.gene062965.t1	MTR_8g461410	68.889	90	24	1	1	86	1	90	6.76e-42	132
MS.gene062965.t1	MTR_4g072240	67.033	91	25	1	1	86	1	91	2.89e-41	130
MS.gene062965.t1	MTR_3g110032	68.132	91	24	1	1	86	1	91	5.60e-41	130
MS.gene062965.t1	MTR_4g072590	77.907	86	18	1	1	86	1	85	7.25e-41	129
MS.gene062965.t1	MTR_4g050700	68.132	91	24	1	1	86	1	91	2.00e-40	128
MS.gene062965.t1	MTR_2g012410	68.132	91	24	1	1	86	1	91	2.00e-40	128
MS.gene062965.t1	MTR_4g072450	77.907	86	18	1	1	86	1	85	3.09e-40	127
MS.gene062965.t1	MTR_4g072300	64.835	91	27	1	1	86	1	91	3.49e-40	127
MS.gene062965.t1	MTR_4g072220	65.934	91	26	1	1	86	1	91	4.50e-40	127
MS.gene062965.t1	MTR_4g072890	64.835	91	27	1	1	86	1	91	7.11e-40	127
MS.gene062965.t1	MTR_4g072880	67.033	91	25	1	1	86	1	91	7.38e-40	127
MS.gene062965.t1	MTR_4g072310	67.033	91	25	1	1	86	1	91	8.42e-40	127
MS.gene062965.t1	MTR_4g072480	65.556	90	27	1	1	86	1	90	8.45e-40	127
MS.gene062965.t1	MTR_4g072380	72.414	87	22	2	1	86	1	86	2.78e-39	125
MS.gene062965.t1	MTR_4g072260	63.736	91	28	1	1	86	1	91	3.47e-39	125
MS.gene062965.t1	MTR_3g110042	68.539	89	24	1	1	85	1	89	4.58e-39	125
MS.gene062965.t1	MTR_4g072250	70.330	91	21	2	1	86	1	90	5.33e-39	125
MS.gene062965.t1	MTR_4g072820	73.563	87	20	2	1	86	1	85	1.08e-38	124
MS.gene062965.t1	MTR_3g084260	65.934	91	26	1	1	86	1	91	1.18e-38	124
MS.gene062965.t1	MTR_4g072620	62.222	90	30	1	1	86	1	90	1.88e-38	123
MS.gene062965.t1	MTR_4g072730	63.333	90	29	1	1	86	1	90	2.06e-38	123
MS.gene062965.t1	MTR_4g072680	63.333	90	29	1	1	86	1	90	4.29e-38	122
MS.gene062965.t1	MTR_4g072850	67.391	92	24	1	1	86	1	92	6.67e-38	122
MS.gene062965.t1	MTR_4g072570	65.556	90	27	1	1	86	1	90	7.02e-38	122
MS.gene062965.t1	MTR_4g072270	63.736	91	28	1	1	86	1	91	7.07e-38	122
MS.gene062965.t1	MTR_3g084250	65.556	90	27	1	1	86	1	90	8.28e-38	122
MS.gene062965.t1	MTR_4g072500	66.667	90	26	1	1	86	1	90	1.05e-37	121
MS.gene062965.t1	MTR_4g072860	69.565	92	21	2	1	86	1	91	1.12e-37	121
MS.gene062965.t1	MTR_4g072530	65.556	90	27	1	1	86	1	90	4.32e-37	120
MS.gene062965.t1	MTR_4g072660	63.953	86	30	1	1	86	1	85	7.99e-37	119
MS.gene062965.t1	MTR_4g072640	65.934	91	25	2	1	86	1	90	1.09e-36	119
MS.gene062965.t1	MTR_4g072460	61.111	90	31	1	1	86	1	90	1.36e-36	119
MS.gene062965.t1	MTR_3g117630	72.603	73	20	0	14	86	18	90	2.74e-36	118
MS.gene062965.t1	MTR_3g084210	64.444	90	28	1	1	86	1	90	1.55e-35	116
MS.gene062965.t1	MTR_3g084190	65.556	90	27	1	1	86	1	90	1.73e-35	115
MS.gene062965.t1	MTR_8g461420	66.667	90	26	1	1	86	1	90	1.84e-35	115
MS.gene062965.t1	MTR_4g072230	64.835	91	25	2	1	86	1	89	1.98e-35	115
MS.gene062965.t1	MTR_3g084230	65.556	90	27	1	1	86	1	90	1.01e-34	114
MS.gene062965.t1	MTR_3g110045	67.045	88	25	1	3	86	62	149	1.57e-34	115
MS.gene062965.t1	MTR_3g084160	64.444	90	28	1	1	86	1	90	2.62e-34	113
MS.gene062965.t1	MTR_5g008370	64.444	90	28	1	1	86	1	90	7.19e-34	113
MS.gene062965.t1	MTR_5g008360	65.385	78	27	0	9	86	4	81	7.30e-34	111
MS.gene062965.t1	MTR_4g072940	63.333	90	27	1	3	86	33	122	1.05e-32	110
MS.gene062965.t1	MTR_3g084200	68.056	72	19	1	1	68	1	72	7.19e-31	104
MS.gene062965.t1	MTR_4g072560	52.577	97	34	3	1	85	1	97	2.70e-30	103
MS.gene062965.t1	MTR_4g072770	59.302	86	29	1	1	86	1	80	6.12e-30	101
MS.gene062965.t1	MTR_4g072910	62.338	77	28	1	10	86	25	100	1.60e-29	101
MS.gene062965.t1	MTR_4g072550	64.789	71	25	0	16	86	31	101	2.06e-29	100
MS.gene062965.t1	MTR_4g072980	52.174	92	37	2	1	85	1	92	7.44e-29	99.4
MS.gene062965.t1	MTR_3g117640	64.706	68	23	1	20	86	33	100	1.18e-28	99.4
MS.gene062965.t1	MTR_4g072290	58.140	86	27	2	1	86	1	77	1.08e-26	93.6
MS.gene062965.t1	MTR_4g072280	58.667	75	26	1	1	70	1	75	1.01e-25	90.9
MS.gene062965.t1	MTR_3g110035	62.500	64	24	0	23	86	73	136	6.15e-25	90.9
MS.gene062965.t1	MTR_4g072750	63.514	74	21	2	1	68	1	74	2.26e-24	87.8
MS.gene062965.t1	MTR_3g117610	58.462	65	26	1	19	83	43	106	1.18e-22	84.3
MS.gene062965.t1	MTR_3g113310	53.731	67	30	1	19	85	40	105	5.55e-22	82.4
MS.gene062965.t1	MTR_6g077850	52.239	67	32	0	20	86	33	99	7.64e-22	81.6
MS.gene062965.t1	MTR_4g072190	50.685	73	35	1	11	83	26	97	1.33e-21	81.3
MS.gene062965.t1	MTR_4g072930	49.383	81	36	2	11	86	30	110	5.89e-21	80.1
MS.gene062965.t1	MTR_8g461400	46.667	90	18	2	1	86	1	64	1.73e-19	74.7
MS.gene062965.t1	MTR_5g021820	45.238	84	43	2	4	85	21	103	4.98e-19	74.7
MS.gene062965.t1	MTR_4g072840	49.425	87	16	3	1	86	1	60	2.03e-18	72.0
MS.gene062965.t1	MTR_8g076040	53.125	64	29	1	20	83	40	102	7.31e-18	72.0
MS.gene062965.t1	MTR_2g043960	55.932	59	25	1	20	78	75	132	2.94e-17	71.6
MS.gene062965.t1	MTR_4g124750	54.237	59	26	1	20	78	68	125	7.59e-17	70.5
MS.gene062965.t1	MTR_1g094750	50.000	62	30	1	20	81	49	109	3.29e-15	65.5
MS.gene062965.t1	MTR_1g061570	46.835	79	39	2	5	81	1	78	3.56e-15	65.5
MS.gene062965.t1	MTR_5g008380	90.625	32	3	0	55	86	246	277	4.11e-15	68.2
MS.gene062965.t1	MTR_1g009150	49.275	69	34	1	13	81	63	130	5.70e-15	66.2
MS.gene062965.t1	MTR_4g005310	46.774	62	32	1	21	81	62	123	2.50e-14	63.9
MS.gene062965.t1	MTR_7g101740	48.438	64	32	1	20	83	39	101	2.69e-14	63.2
MS.gene062965.t1	MTR_1g063950	45.161	62	33	1	20	81	47	107	3.26e-14	63.2
MS.gene062965.t1	MTR_7g118010	49.231	65	30	2	21	83	23	86	4.85e-14	62.0
MS.gene062965.t1	MTR_3g105900	66.667	42	14	0	20	61	1171	1212	1.02e-13	65.1
MS.gene062965.t1	MTR_4g025830	42.254	71	37	2	18	85	76	145	1.48e-13	62.4
MS.gene062965.t1	MTR_4g005320	47.541	61	31	1	21	81	70	129	1.68e-13	62.0
MS.gene062965.t1	MTR_1g024085	50.847	59	28	1	20	78	36	93	2.40e-13	61.2
MS.gene062965.t1	MTR_0388s0020	39.744	78	43	2	11	85	65	141	2.77e-13	61.2
MS.gene062965.t1	MTR_3g092220	43.478	69	36	2	12	78	24	91	3.42e-13	60.1
MS.gene062965.t1	MTR_8g461210	42.105	76	39	2	7	78	17	91	5.83e-13	59.7
MS.gene062965.t1	MTR_3g109160	49.180	61	30	1	21	81	69	128	2.41e-12	59.3
MS.gene062965.t1	MTR_4g072160	44.000	75	40	2	8	81	23	96	2.57e-12	58.5
MS.gene062965.t1	MTR_8g026730	44.444	63	32	2	21	81	27	88	9.05e-12	56.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062965.t1	AT4G38840	72.059	68	18	1	20	86	32	99	4.56e-33	110
MS.gene062965.t1	AT4G34800	57.143	84	33	1	1	81	1	84	4.25e-30	102
MS.gene062965.t1	AT2G21210	54.321	81	35	1	8	86	15	95	5.20e-30	102
MS.gene062965.t1	AT2G21210	54.321	81	35	1	8	86	82	162	2.90e-29	102
MS.gene062965.t1	AT5G18080	58.108	74	30	1	14	86	17	90	1.15e-28	99.0
MS.gene062965.t1	AT4G34810	60.256	78	27	1	10	83	37	114	2.39e-28	99.0
MS.gene062965.t1	AT4G38850	54.878	82	29	2	8	86	13	89	6.22e-28	97.1
MS.gene062965.t1	AT4G38825	56.410	78	28	2	10	86	17	89	8.45e-28	96.7
MS.gene062965.t1	AT5G18020	56.757	74	31	1	14	86	17	90	8.65e-28	96.7
MS.gene062965.t1	AT5G18050	56.410	78	30	2	10	86	16	90	1.23e-27	96.3
MS.gene062965.t1	AT5G18060	62.121	66	24	1	22	86	25	90	1.28e-27	96.3
MS.gene062965.t1	AT5G18030	55.696	79	31	2	9	86	13	88	1.91e-27	95.5
MS.gene062965.t1	AT2G21200	58.333	72	29	1	15	85	15	86	2.96e-27	95.1
MS.gene062965.t1	AT5G18010	55.405	74	32	1	14	86	17	90	6.89e-27	94.4
MS.gene062965.t1	AT4G34770	57.143	84	34	1	5	86	19	102	4.04e-26	92.8
MS.gene062965.t1	AT3G03820	54.430	79	33	1	11	86	17	95	1.40e-25	91.3
MS.gene062965.t1	AT4G13790	48.235	85	41	2	3	85	8	91	4.14e-25	89.7
MS.gene062965.t1	AT3G03840	52.564	78	35	1	11	86	17	94	8.30e-25	89.4
MS.gene062965.t1	AT4G34790	67.164	67	19	2	21	84	39	105	9.02e-25	89.4
MS.gene062965.t1	AT3G03850	59.701	67	25	1	22	86	26	92	1.56e-24	88.6
MS.gene062965.t1	AT3G03830	54.430	79	30	2	11	86	17	92	1.73e-23	85.9
MS.gene062965.t1	AT4G38860	48.235	85	42	1	4	86	21	105	3.13e-23	85.5
MS.gene062965.t1	AT2G21220	47.561	82	40	2	4	83	21	101	1.75e-21	81.3
MS.gene062965.t1	AT4G34780	55.714	70	28	1	19	85	26	95	3.02e-21	80.5
MS.gene062965.t1	AT1G75580	56.716	67	28	1	19	85	42	107	4.01e-21	80.1
MS.gene062965.t1	AT4G34760	55.385	65	28	1	19	83	41	104	1.88e-20	78.6
MS.gene062965.t1	AT2G16580	55.385	65	28	1	19	83	42	105	5.09e-20	77.4
MS.gene062965.t1	AT4G36110	51.562	64	30	1	20	83	39	101	2.86e-18	72.8
MS.gene062965.t1	AT1G19830	57.353	68	27	2	19	85	46	112	4.83e-18	72.8
MS.gene062965.t1	AT2G18010	51.562	64	30	1	20	83	47	109	7.51e-18	72.0
MS.gene062965.t1	AT3G43120	54.237	59	26	1	20	78	78	135	1.36e-17	72.8
MS.gene062965.t1	AT5G20810	55.932	59	25	1	20	78	78	135	9.70e-17	70.5
MS.gene062965.t1	AT5G20810	55.932	59	25	1	20	78	78	135	1.43e-16	70.9
MS.gene062965.t1	AT3G20220	48.387	62	30	2	21	81	47	107	9.57e-15	64.3
MS.gene062965.t1	AT5G66260	48.485	66	33	1	21	86	34	98	1.88e-14	63.2
MS.gene062965.t1	AT2G37030	49.206	63	31	1	20	82	48	109	4.29e-14	62.8
MS.gene062965.t1	AT1G75590	47.458	59	30	1	20	78	41	98	5.40e-13	60.5
MS.gene062965.t1	AT3G51200	42.683	82	41	3	5	81	19	99	6.31e-13	59.3
MS.gene062965.t1	AT3G61900	40.506	79	42	3	12	86	20	97	8.49e-13	59.7
MS.gene062965.t1	AT5G10990	42.667	75	37	2	4	78	29	97	1.15e-12	59.7
MS.gene062965.t1	AT2G46690	42.857	77	40	2	5	78	8	83	1.23e-12	58.9
MS.gene062965.t1	AT3G53250	44.444	63	31	1	20	82	51	109	1.69e-12	58.5
MS.gene062965.t1	AT4G34750	42.188	64	36	1	20	83	36	98	2.15e-12	58.9
MS.gene062965.t1	AT4G34750	42.188	64	36	1	20	83	36	98	2.15e-12	58.9
MS.gene062965.t1	AT3G53250	44.444	63	31	1	20	82	82	140	3.04e-12	58.5
MS.gene062965.t1	AT1G19840	44.000	75	36	2	4	78	29	97	3.66e-12	58.5
MS.gene062965.t1	AT4G31320	45.902	61	32	1	21	81	80	139	1.49e-11	57.4
MS.gene062965.t1	AT2G24400	47.541	61	31	1	21	81	64	123	2.35e-11	57.0
MS.gene062965.t1	AT4G00880	42.308	78	39	3	3	78	13	86	2.64e-11	55.5
MS.gene062965.t1	AT2G45210	48.214	56	25	2	21	73	79	133	4.43e-11	55.8

Find 22 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAGCTTGGTTCAACAGTTCT+TGG	0.219724	4.2:+35487880	None:intergenic
GTTTGGTTCAAATATGATAT+AGG	0.322369	4.2:+35487909	None:intergenic
GGCATCATCTTCAAAAGGTT+TGG	0.355226	4.2:-35487991	MS.gene062965:CDS
TTTGGTTCAAATATGATATA+GGG	0.362947	4.2:+35487910	None:intergenic
ACAGTTCTTGGAATGAGGTT+TGG	0.410916	4.2:+35487892	None:intergenic
TACGCTGCTAGATACCCTTT+TGG	0.421486	4.2:+35487963	None:intergenic
ATCTGATGATACTTGGTAAA+CGG	0.429253	4.2:+35488018	None:intergenic
ATTCAAATTGTTCTTCAGCT+TGG	0.462367	4.2:+35487865	None:intergenic
AAAGGTTTGGACGTGCCAAA+AGG	0.464288	4.2:-35487978	MS.gene062965:CDS
GTTCAACAGTTCTTGGAATG+AGG	0.477913	4.2:+35487887	None:intergenic
TCAAGGGCATCATCTTCAAA+AGG	0.490872	4.2:-35487996	MS.gene062965:CDS
TACCAAGTATCATCAGATCA+AGG	0.490956	4.2:-35488013	MS.gene062965:CDS
GCCCTTGATCTGATGATACT+TGG	0.512708	4.2:+35488011	None:intergenic
AAGGTTTGGACGTGCCAAAA+GGG	0.538442	4.2:-35487977	MS.gene062965:CDS
TTTGAATATGACCATCCAAT+GGG	0.542663	4.2:-35487849	MS.gene062965:CDS
ATTTGAATATGACCATCCAA+TGG	0.559485	4.2:-35487850	MS.gene062965:CDS
AAGAAAATCTCTTCTCTGCA+AGG	0.560279	4.2:+35487813	None:intergenic
TAGTTAGACCACCCATTGGA+TGG	0.598853	4.2:+35487838	None:intergenic
ACCAAGTATCATCAGATCAA+GGG	0.600732	4.2:-35488012	MS.gene062965:CDS
GGAATAGTTAGACCACCCAT+TGG	0.610953	4.2:+35487834	None:intergenic
GGGTATCTAGCAGCGTACGT+AGG	0.631566	4.2:-35487957	MS.gene062965:CDS
GAATATGACCATCCAATGGG+TGG	0.646056	4.2:-35487846	MS.gene062965:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTGGTTCAAATATGATATA+GGG	+	chr4.2:35487907-35487926	None:intergenic	20.0%
!!	GTTTGGTTCAAATATGATAT+AGG	+	chr4.2:35487908-35487927	None:intergenic	25.0%
	ATCTGATGATACTTGGTAAA+CGG	+	chr4.2:35487799-35487818	None:intergenic	30.0%
	ATTCAAATTGTTCTTCAGCT+TGG	+	chr4.2:35487952-35487971	None:intergenic	30.0%
	ATTTGAATATGACCATCCAA+TGG	-	chr4.2:35487964-35487983	MS.gene062965:CDS	30.0%
	TTTGAATATGACCATCCAAT+GGG	-	chr4.2:35487965-35487984	MS.gene062965:CDS	30.0%
	AAGAAAATCTCTTCTCTGCA+AGG	+	chr4.2:35488004-35488023	None:intergenic	35.0%
	ACCAAGTATCATCAGATCAA+GGG	-	chr4.2:35487802-35487821	MS.gene062965:CDS	35.0%
	ACGTAGGAGAAAAAATGAAG+CGG	-	chr4.2:35487873-35487892	MS.gene062965:CDS	35.0%
	TACCAAGTATCATCAGATCA+AGG	-	chr4.2:35487801-35487820	MS.gene062965:CDS	35.0%
	ACAGTTCTTGGAATGAGGTT+TGG	+	chr4.2:35487925-35487944	None:intergenic	40.0%
	GGCATCATCTTCAAAAGGTT+TGG	-	chr4.2:35487823-35487842	MS.gene062965:CDS	40.0%
	GTTCAACAGTTCTTGGAATG+AGG	+	chr4.2:35487930-35487949	None:intergenic	40.0%
!!	TCAAGGGCATCATCTTCAAA+AGG	-	chr4.2:35487818-35487837	MS.gene062965:CDS	40.0%
	AAAGGTTTGGACGTGCCAAA+AGG	-	chr4.2:35487836-35487855	MS.gene062965:CDS	45.0%
	AAGGTTTGGACGTGCCAAAA+GGG	-	chr4.2:35487837-35487856	MS.gene062965:CDS	45.0%
	CAGCTTGGTTCAACAGTTCT+TGG	+	chr4.2:35487937-35487956	None:intergenic	45.0%
	GAATATGACCATCCAATGGG+TGG	-	chr4.2:35487968-35487987	MS.gene062965:CDS	45.0%
	GCCCTTGATCTGATGATACT+TGG	+	chr4.2:35487806-35487825	None:intergenic	45.0%
	GGAATAGTTAGACCACCCAT+TGG	+	chr4.2:35487983-35488002	None:intergenic	45.0%
	TAGTTAGACCACCCATTGGA+TGG	+	chr4.2:35487979-35487998	None:intergenic	45.0%
!	TACGCTGCTAGATACCCTTT+TGG	+	chr4.2:35487854-35487873	None:intergenic	45.0%
	GGGTATCTAGCAGCGTACGT+AGG	-	chr4.2:35487857-35487876	MS.gene062965:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	35487788	35488048	35487788	ID=MS.gene062965
chr4.2	mRNA	35487788	35488048	35487788	ID=MS.gene062965.t1;Parent=MS.gene062965
chr4.2	exon	35487788	35488048	35487788	ID=MS.gene062965.t1.exon1;Parent=MS.gene062965.t1
chr4.2	CDS	35487788	35488048	35487788	ID=cds.MS.gene062965.t1;Parent=MS.gene062965.t1

Gene Sequence	>MS.gene062965 ATGGGTTTCCGTTTACCAAGTATCATCAGATCAAGGGCATCATCTTCAAAAGGTTTGGACGTGCCAAAAGGGTATCTAGCAGCGTACGTAGGAGAAAAAATGAAGCGGTTTGTGATCCCTATATCATATTTGAACCAAACCTCATTCCAAGAACTGTTGAACCAAGCTGAAGAACAATTTGAATATGACCATCCAATGGGTGGTCTAACTATTCCTTGCAGAGAAGAGATTTTCTTAGATATCACTTCTCACTTGAATTGA
Protein sequence	>MS.gene062965.t1 MGFRLPSIIRSRASSSKGLDVPKGYLAAYVGEKMKRFVIPISYLNQTSFQELLNQAEEQFEYDHPMGGLTIPCREEIFLDITSHLN