AlfalfaGEDB | MS.gene062966

M. sativa cultivar XinJiangDaYe / MS.gene062966

KEGG: K14488
GO: GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
PFAM: PF02519.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062966.t1	XP_003607131.1	94.3	87	5	0	1	87	1	87	7.50E-41	176

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062966.t1	P33080	72.5	91	18	2	1	85	1	90	5.4e-32	137.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062966.t1	G7JL53	94.3	87	5	0	1	87	1	87	5.4e-41	176.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene057823	MS.gene062966	0.800954	1.13E-48	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene062966	MS.gene75679	PPI
MS.gene062966	MS.gene68761	PPI
MS.gene062966	MS.gene40325	PPI
MS.gene77523	MS.gene062966	PPI
MS.gene057778	MS.gene062966	PPI
MS.gene062966	MS.gene50677	PPI
MS.gene062966	MS.gene50684	PPI
MS.gene062966	MS.gene057797	PPI
MS.gene062966	MS.gene003614	PPI
MS.gene062966	MS.gene23372	PPI
MS.gene062966	MS.gene014668	PPI
MS.gene062966	MS.gene79886	PPI
MS.gene062966	MS.gene50765	PPI
MS.gene79890	MS.gene062966	PPI
MS.gene062966	MS.gene003596	PPI
MS.gene003615	MS.gene062966	PPI
MS.gene062966	MS.gene062988	PPI
MS.gene062966	MS.gene23371	PPI
MS.gene062973	MS.gene062966	PPI
MS.gene003602	MS.gene062966	PPI
MS.gene047517	MS.gene062966	PPI
MS.gene062966	MS.gene003583	PPI
MS.gene062966	MS.gene062951	PPI
MS.gene062984	MS.gene062966	PPI
MS.gene062966	MS.gene77527	PPI
MS.gene062966	MS.gene79891	PPI
MS.gene062966	MS.gene057795	PPI
MS.gene062966	MS.gene045367	PPI
MS.gene062966	MS.gene045364	PPI
MS.gene40327	MS.gene062966	PPI
MS.gene062966	MS.gene045363	PPI
MS.gene062966	MS.gene50652	PPI
MS.gene062966	MS.gene014669	PPI
MS.gene062966	MS.gene057808	PPI
MS.gene062966	MS.gene002380	PPI
MS.gene062966	MS.gene68165	PPI
MS.gene003587	MS.gene062966	PPI
MS.gene062966	MS.gene77526	PPI
MS.gene062966	MS.gene045366	PPI
MS.gene003590	MS.gene062966	PPI
MS.gene062943	MS.gene062966	PPI
MS.gene063112	MS.gene062966	PPI
MS.gene062966	MS.gene68163	PPI
MS.gene062966	MS.gene045362	PPI
MS.gene003608	MS.gene062966	PPI
MS.gene062966	MS.gene75315	PPI
MS.gene062966	MS.gene057818	PPI
MS.gene062966	MS.gene68164	PPI
MS.gene062966	MS.gene68759	PPI
MS.gene062966	MS.gene003586	PPI
MS.gene062966	MS.gene003627	PPI
MS.gene062966	MS.gene031200	PPI
MS.gene062966	MS.gene79888	PPI
MS.gene062966	MS.gene75676	PPI
MS.gene062966	MS.gene031199	PPI
MS.gene062966	MS.gene50687	PPI
MS.gene75677	MS.gene062966	PPI
MS.gene062966	MS.gene014223	PPI
MS.gene003625	MS.gene062966	PPI
MS.gene062966	MS.gene79885	PPI
MS.gene045361	MS.gene062966	PPI
MS.gene003591	MS.gene062966	PPI
MS.gene062966	MS.gene062963	PPI
MS.gene062979	MS.gene062966	PPI
MS.gene01358	MS.gene062966	PPI
MS.gene40326	MS.gene062966	PPI
MS.gene062966	MS.gene79889	PPI
MS.gene062952	MS.gene062966	PPI
MS.gene062966	MS.gene045368	PPI
MS.gene062966	MS.gene045360	PPI
MS.gene062966	MS.gene014222	PPI
MS.gene50676	MS.gene062966	PPI
MS.gene062966	MS.gene75678	PPI
MS.gene23370	MS.gene062966	PPI
MS.gene50766	MS.gene062966	PPI
MS.gene062966	MS.gene75314	PPI
MS.gene77529	MS.gene062966	PPI
MS.gene062966	MS.gene01764	PPI
MS.gene014667	MS.gene062966	PPI
MS.gene003594	MS.gene062966	PPI
MS.gene50665	MS.gene062966	PPI
MS.gene062966	MS.gene75673	PPI
MS.gene062966	MS.gene68760	PPI
MS.gene062966	MS.gene031198	PPI
MS.gene062966	MS.gene50764	PPI
MS.gene50763	MS.gene062966	PPI
MS.gene062966	MS.gene77528	PPI
MS.gene062966	MS.gene014671	PPI
MS.gene045365	MS.gene062966	PPI
MS.gene062966	MS.gene003577	PPI
MS.gene062966	MS.gene75672	PPI
MS.gene062966	MS.gene75674	PPI
MS.gene50659	MS.gene062966	PPI
MS.gene062966	MS.gene50654	PPI
MS.gene062966	MS.gene50675	PPI
MS.gene75316	MS.gene062966	PPI
MS.gene062966	MS.gene062974	PPI
MS.gene057790	MS.gene062966	PPI
MS.gene062966	MS.gene014670	PPI
MS.gene062966	MS.gene057801	PPI
MS.gene062966	MS.gene77524	PPI
MS.gene003624	MS.gene062966	PPI
MS.gene062966	MS.gene68162	PPI
MS.gene031201	MS.gene062966	PPI
MS.gene062966	MS.gene75675	PPI
MS.gene062966	MS.gene79887	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062966.t1	MTR_4g072580	94.253	87	5	0	1	87	1	87	3.59e-58	173
MS.gene062966.t1	MTR_4g072600	91.954	87	7	0	1	87	1	87	3.83e-55	165
MS.gene062966.t1	MTR_4g072490	84.706	85	13	0	1	85	1	85	1.42e-51	156
MS.gene062966.t1	MTR_4g072740	83.529	85	14	0	1	85	1	85	2.69e-51	155
MS.gene062966.t1	MTR_4g072830	85.393	89	10	1	1	86	1	89	2.74e-51	155
MS.gene062966.t1	MTR_4g072370	83.333	90	12	1	1	87	1	90	3.76e-51	155
MS.gene062966.t1	MTR_4g072470	82.353	85	15	0	1	85	1	85	6.50e-49	149
MS.gene062966.t1	MTR_4g072630	82.558	86	14	1	1	85	1	86	7.08e-49	149
MS.gene062966.t1	MTR_4g072610	81.609	87	15	1	1	87	1	86	1.01e-48	149
MS.gene062966.t1	MTR_4g072330	76.667	90	18	1	1	87	1	90	5.28e-47	145
MS.gene062966.t1	MTR_4g072650	79.310	87	17	1	1	87	1	86	7.60e-47	144
MS.gene062966.t1	MTR_4g072465	79.070	86	17	1	1	85	1	86	1.67e-46	143
MS.gene062966.t1	MTR_4g072510	84.884	86	12	1	1	85	1	86	1.96e-45	140
MS.gene062966.t1	MTR_4g072540	83.721	86	13	1	1	85	1	86	2.94e-45	140
MS.gene062966.t1	MTR_5g008350	86.486	74	10	0	1	74	1	74	1.22e-44	138
MS.gene062966.t1	MTR_4g072670	82.558	86	14	1	1	85	1	86	1.80e-44	138
MS.gene062966.t1	MTR_3g084150	74.725	91	16	2	1	85	1	90	2.20e-44	138
MS.gene062966.t1	MTR_3g084180	73.626	91	17	2	1	85	1	90	1.30e-43	136
MS.gene062966.t1	MTR_4g072820	80.000	85	15	2	1	84	1	84	2.22e-43	135
MS.gene062966.t1	MTR_4g072880	71.429	91	20	1	1	85	1	91	3.40e-43	135
MS.gene062966.t1	MTR_4g072310	70.330	91	21	1	1	85	1	91	9.03e-43	134
MS.gene062966.t1	MTR_8g461410	72.527	91	18	2	1	85	1	90	9.67e-43	134
MS.gene062966.t1	MTR_3g084170	70.000	90	22	1	1	85	1	90	1.79e-42	133
MS.gene062966.t1	MTR_3g110038	70.000	90	22	1	1	85	1	90	2.15e-42	133
MS.gene062966.t1	MTR_4g072870	68.132	91	23	1	1	85	1	91	2.69e-42	133
MS.gene062966.t1	MTR_4g072720	79.070	86	17	1	1	85	1	86	4.79e-42	132
MS.gene062966.t1	MTR_4g072380	75.904	83	19	1	1	82	1	83	5.33e-42	132
MS.gene062966.t1	MTR_4g072690	80.000	85	17	0	1	85	1	85	1.29e-41	131
MS.gene062966.t1	MTR_3g084240	68.889	90	23	1	1	85	1	90	1.66e-41	131
MS.gene062966.t1	MTR_3g110032	68.132	91	23	1	1	85	1	91	3.56e-41	130
MS.gene062966.t1	MTR_4g072220	68.132	91	23	1	1	85	1	91	3.93e-41	130
MS.gene062966.t1	MTR_4g072240	65.934	91	25	1	1	85	1	91	7.65e-41	129
MS.gene062966.t1	MTR_4g072250	71.429	91	19	2	1	85	1	90	8.41e-41	129
MS.gene062966.t1	MTR_4g072480	67.778	90	24	1	1	85	1	90	2.28e-40	128
MS.gene062966.t1	MTR_4g072450	78.824	85	18	0	1	85	1	85	2.73e-40	128
MS.gene062966.t1	MTR_4g072590	78.824	85	18	0	1	85	1	85	2.76e-40	127
MS.gene062966.t1	MTR_4g072260	65.934	91	25	1	1	85	1	91	2.96e-40	128
MS.gene062966.t1	MTR_3g084220	66.667	90	25	1	1	85	1	90	3.62e-40	127
MS.gene062966.t1	MTR_3g084250	67.778	90	24	1	1	85	1	90	5.20e-40	127
MS.gene062966.t1	MTR_4g072230	71.910	89	17	2	1	83	1	87	5.82e-40	127
MS.gene062966.t1	MTR_4g050700	67.033	91	24	1	1	85	1	91	7.69e-40	127
MS.gene062966.t1	MTR_2g012410	67.033	91	24	1	1	85	1	91	7.69e-40	127
MS.gene062966.t1	MTR_3g110042	73.864	88	16	2	1	82	1	87	8.07e-40	127
MS.gene062966.t1	MTR_4g072300	64.835	91	26	1	1	85	1	91	8.27e-40	127
MS.gene062966.t1	MTR_4g072660	69.512	82	25	0	1	82	1	82	1.37e-39	126
MS.gene062966.t1	MTR_4g072890	64.835	91	26	1	1	85	1	91	1.45e-39	126
MS.gene062966.t1	MTR_4g072270	67.045	88	23	1	1	82	1	88	1.83e-39	126
MS.gene062966.t1	MTR_4g072620	65.556	90	26	1	1	85	1	90	1.96e-39	125
MS.gene062966.t1	MTR_4g072680	66.667	90	25	1	1	85	1	90	4.00e-39	125
MS.gene062966.t1	MTR_3g117630	72.289	83	17	1	9	85	8	90	6.48e-39	124
MS.gene062966.t1	MTR_4g072570	67.778	90	24	1	1	85	1	90	6.69e-39	124
MS.gene062966.t1	MTR_4g072730	65.556	90	26	1	1	85	1	90	7.39e-39	124
MS.gene062966.t1	MTR_4g072860	69.231	91	22	1	1	85	1	91	1.25e-38	124
MS.gene062966.t1	MTR_3g084260	65.934	91	25	1	1	85	1	91	2.16e-38	123
MS.gene062966.t1	MTR_4g072850	69.892	93	21	1	1	86	1	93	4.20e-38	122
MS.gene062966.t1	MTR_4g072460	64.444	90	27	1	1	85	1	90	8.16e-38	122
MS.gene062966.t1	MTR_8g461420	67.033	91	25	1	1	86	1	91	8.81e-38	122
MS.gene062966.t1	MTR_3g084190	64.835	91	27	1	1	86	1	91	2.67e-37	120
MS.gene062966.t1	MTR_4g072500	64.835	91	25	2	1	85	1	90	3.79e-37	120
MS.gene062966.t1	MTR_3g084230	65.934	91	26	1	1	86	1	91	4.05e-37	120
MS.gene062966.t1	MTR_4g072640	64.130	92	26	2	1	86	1	91	1.61e-36	118
MS.gene062966.t1	MTR_3g084210	64.444	90	27	1	1	85	1	90	4.00e-36	117
MS.gene062966.t1	MTR_4g072530	62.637	91	27	2	1	85	1	90	4.92e-36	117
MS.gene062966.t1	MTR_3g110045	66.292	89	25	1	3	86	62	150	5.13e-36	119
MS.gene062966.t1	MTR_3g084160	62.637	91	29	1	1	86	1	91	5.15e-35	114
MS.gene062966.t1	MTR_4g072940	68.132	91	22	1	3	86	33	123	5.65e-35	115
MS.gene062966.t1	MTR_5g008360	69.333	75	23	0	11	85	7	81	1.20e-34	113
MS.gene062966.t1	MTR_4g072980	56.522	92	32	2	1	84	1	92	2.94e-33	110
MS.gene062966.t1	MTR_5g008370	76.812	69	16	0	17	85	22	90	1.46e-32	110
MS.gene062966.t1	MTR_4g072560	55.670	97	30	3	1	84	1	97	7.02e-32	107
MS.gene062966.t1	MTR_3g084200	68.056	72	18	1	1	67	1	72	9.77e-32	107
MS.gene062966.t1	MTR_4g072770	67.059	85	21	2	1	84	1	79	1.08e-31	106
MS.gene062966.t1	MTR_4g072550	69.014	71	22	0	15	85	31	101	1.59e-31	106
MS.gene062966.t1	MTR_3g117640	65.714	70	23	1	19	87	33	102	2.42e-31	105
MS.gene062966.t1	MTR_4g072910	65.333	75	25	1	11	85	27	100	5.96e-31	105
MS.gene062966.t1	MTR_4g072290	54.118	85	31	1	1	85	1	77	7.40e-27	94.0
MS.gene062966.t1	MTR_3g110035	64.062	64	23	0	22	85	73	136	3.93e-26	94.0
MS.gene062966.t1	MTR_4g072280	58.667	75	25	1	1	69	1	75	2.37e-25	90.1
MS.gene062966.t1	MTR_4g072750	63.514	74	20	2	1	67	1	74	4.41e-25	89.7
MS.gene062966.t1	MTR_6g077850	57.353	68	29	0	19	86	33	100	2.22e-24	88.2
MS.gene062966.t1	MTR_3g113310	55.224	67	29	1	18	84	40	105	1.49e-23	86.3
MS.gene062966.t1	MTR_4g072190	51.190	84	36	2	5	84	17	99	2.46e-23	85.5
MS.gene062966.t1	MTR_3g117610	56.522	69	29	1	18	86	43	110	3.50e-23	85.5
MS.gene062966.t1	MTR_4g072930	50.000	80	36	1	10	85	31	110	3.05e-22	83.2
MS.gene062966.t1	MTR_5g021820	48.052	77	37	2	10	84	28	103	1.46e-19	76.3
MS.gene062966.t1	MTR_4g072840	52.941	85	13	3	1	84	1	59	1.61e-19	74.7
MS.gene062966.t1	MTR_8g076040	57.627	59	24	1	19	77	40	97	2.17e-18	73.2
MS.gene062966.t1	MTR_8g461400	44.444	90	19	2	1	85	1	64	1.19e-17	70.1
MS.gene062966.t1	MTR_1g094750	53.226	62	28	1	19	80	49	109	2.09e-17	70.9
MS.gene062966.t1	MTR_2g043960	57.627	59	24	1	19	77	75	132	2.14e-17	72.4
MS.gene062966.t1	MTR_1g009150	53.623	69	31	1	12	80	63	130	2.90e-17	72.4
MS.gene062966.t1	MTR_4g124750	55.932	59	25	1	19	77	68	125	8.87e-17	70.5
MS.gene062966.t1	MTR_4g025830	44.444	72	36	2	17	85	76	146	3.39e-16	68.9
MS.gene062966.t1	MTR_7g118010	41.667	84	46	2	5	86	8	90	3.62e-16	67.4
MS.gene062966.t1	MTR_1g063950	47.059	68	35	1	13	80	41	107	3.66e-16	68.2
MS.gene062966.t1	MTR_3g092220	42.857	77	41	2	3	77	16	91	1.64e-15	66.2
MS.gene062966.t1	MTR_7g101740	50.746	67	32	1	19	85	39	104	1.70e-15	66.2
MS.gene062966.t1	MTR_3g105900	71.429	42	12	0	19	60	1171	1212	2.07e-15	69.7
MS.gene062966.t1	MTR_4g005310	50.000	62	30	1	20	80	62	123	3.33e-15	66.2
MS.gene062966.t1	MTR_8g461210	52.542	59	27	1	19	77	34	91	3.45e-15	65.5
MS.gene062966.t1	MTR_0388s0020	42.308	78	41	2	11	85	66	142	4.10e-15	65.9
MS.gene062966.t1	MTR_1g061570	53.226	62	28	1	19	80	18	78	5.00e-15	65.1
MS.gene062966.t1	MTR_4g005320	50.820	61	29	1	20	80	70	129	1.38e-14	65.1
MS.gene062966.t1	MTR_1g024085	43.902	82	37	2	4	77	13	93	1.48e-14	64.3
MS.gene062966.t1	MTR_8g026730	43.478	69	36	2	20	86	27	94	3.46e-14	62.8
MS.gene062966.t1	MTR_4g072160	44.156	77	42	1	10	86	27	102	6.14e-14	62.8
MS.gene062966.t1	MTR_3g109160	50.820	61	29	1	20	80	69	128	2.61e-13	61.6
MS.gene062966.t1	MTR_7g405740	38.158	76	46	1	5	80	52	126	1.40e-12	59.7
MS.gene062966.t1	MTR_4g021810	49.254	67	32	2	12	77	18	83	5.93e-12	56.6
MS.gene062966.t1	MTR_2g066830	38.158	76	45	2	2	77	41	114	6.18e-12	57.8
MS.gene062966.t1	MTR_5g008380	78.125	32	7	0	54	85	246	277	7.22e-12	59.3
MS.gene062966.t1	MTR_8g101520	43.939	66	36	1	21	86	51	115	9.44e-12	57.4
MS.gene062966.t1	MTR_2g095430	45.833	72	32	3	8	73	31	101	1.25e-11	56.6
MS.gene062966.t1	MTR_3g097120	41.772	79	37	2	16	86	5	82	1.35e-11	55.8
MS.gene062966.t1	MTR_1g059770	39.344	61	36	1	20	80	49	108	6.29e-11	54.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062966.t1	AT4G38840	75.000	68	16	1	19	85	32	99	3.14e-34	113
MS.gene062966.t1	AT2G21210	63.158	76	26	1	12	85	20	95	7.07e-32	107
MS.gene062966.t1	AT5G18020	60.000	80	30	2	8	86	13	91	1.00e-31	106
MS.gene062966.t1	AT5G18080	62.821	78	27	2	8	84	13	89	1.07e-31	106
MS.gene062966.t1	AT2G21210	63.158	76	26	1	12	85	87	162	3.26e-31	107
MS.gene062966.t1	AT2G21200	60.494	81	29	2	7	84	6	86	4.39e-31	104
MS.gene062966.t1	AT5G18050	58.974	78	30	2	8	84	13	89	8.60e-31	104
MS.gene062966.t1	AT5G18060	60.256	78	29	2	8	84	13	89	1.35e-30	103
MS.gene062966.t1	AT5G18030	60.256	78	27	2	8	84	13	87	1.77e-30	103
MS.gene062966.t1	AT5G18010	60.256	78	29	2	8	84	13	89	2.81e-30	102
MS.gene062966.t1	AT4G34800	58.333	84	31	2	1	80	1	84	1.16e-29	101
MS.gene062966.t1	AT4G34810	65.823	79	22	2	11	84	39	117	1.65e-29	102
MS.gene062966.t1	AT4G38825	60.563	71	27	1	15	84	18	88	2.05e-29	100
MS.gene062966.t1	AT4G38850	57.500	80	27	2	8	84	13	88	5.21e-29	99.8
MS.gene062966.t1	AT4G34770	65.333	75	24	1	13	85	28	102	6.25e-29	100
MS.gene062966.t1	AT3G03840	54.651	86	31	2	1	84	14	93	3.40e-28	97.8
MS.gene062966.t1	AT3G03850	58.537	82	28	2	5	84	14	91	4.17e-27	95.1
MS.gene062966.t1	AT4G13790	46.429	84	43	1	3	84	8	91	5.16e-27	94.7
MS.gene062966.t1	AT3G03820	55.263	76	31	1	12	84	19	94	2.24e-26	93.2
MS.gene062966.t1	AT4G34780	61.111	72	25	1	18	86	26	97	1.16e-25	91.7
MS.gene062966.t1	AT3G03830	55.422	83	29	2	5	84	14	91	1.22e-25	91.3
MS.gene062966.t1	AT4G34790	67.213	61	18	1	20	78	39	99	1.17e-24	89.4
MS.gene062966.t1	AT4G38860	55.224	67	30	0	18	84	38	104	2.29e-23	85.9
MS.gene062966.t1	AT2G21220	53.731	67	30	1	18	84	38	103	1.26e-21	81.6
MS.gene062966.t1	AT1G75580	56.716	67	28	1	18	84	42	107	1.98e-21	81.3
MS.gene062966.t1	AT4G34760	53.731	67	30	1	18	84	41	106	6.20e-21	79.7
MS.gene062966.t1	AT1G19830	60.000	60	23	1	18	77	46	104	4.89e-19	75.1
MS.gene062966.t1	AT2G16580	53.846	65	29	1	18	82	42	105	1.26e-18	73.9
MS.gene062966.t1	AT4G36110	47.674	86	36	3	5	82	17	101	1.74e-18	73.6
MS.gene062966.t1	AT3G20220	50.725	69	32	2	20	87	47	114	1.19e-17	71.6
MS.gene062966.t1	AT3G43120	55.932	59	25	1	19	77	78	135	1.19e-17	72.8
MS.gene062966.t1	AT2G18010	55.932	59	25	1	19	77	47	104	2.97e-17	70.5
MS.gene062966.t1	AT5G20810	57.627	59	24	1	19	77	78	135	8.48e-17	70.9
MS.gene062966.t1	AT5G20810	57.627	59	24	1	19	77	78	135	1.67e-16	70.5
MS.gene062966.t1	AT2G37030	54.839	62	27	1	19	80	48	108	2.26e-16	68.6
MS.gene062966.t1	AT4G34750	44.928	69	37	1	19	87	36	103	2.43e-15	66.6
MS.gene062966.t1	AT4G34750	44.928	69	37	1	19	87	36	103	2.43e-15	66.6
MS.gene062966.t1	AT3G51200	46.154	78	36	3	8	80	23	99	2.81e-15	65.5
MS.gene062966.t1	AT5G66260	44.944	89	40	3	5	85	11	98	5.24e-15	64.7
MS.gene062966.t1	AT1G75590	50.847	59	28	1	19	77	41	98	6.93e-15	65.5
MS.gene062966.t1	AT3G61900	41.250	80	42	3	11	86	20	98	1.15e-14	64.7
MS.gene062966.t1	AT2G46690	45.833	72	35	2	9	77	13	83	1.49e-14	63.9
MS.gene062966.t1	AT5G10990	49.153	59	29	1	19	77	40	97	2.06e-14	64.3
MS.gene062966.t1	AT3G53250	48.387	62	28	1	19	80	51	108	2.09e-14	63.5
MS.gene062966.t1	AT3G53250	48.387	62	28	1	19	80	82	139	3.81e-14	63.5
MS.gene062966.t1	AT1G19840	45.783	83	35	3	4	77	16	97	3.82e-14	63.5
MS.gene062966.t1	AT4G22620	38.554	83	47	2	6	85	65	146	1.70e-13	62.0
MS.gene062966.t1	AT4G31320	46.575	73	37	2	9	80	68	139	2.23e-13	62.4
MS.gene062966.t1	AT2G24400	46.250	80	41	2	2	80	45	123	2.79e-13	62.0
MS.gene062966.t1	AT4G12410	43.478	69	35	2	20	85	76	143	5.55e-13	60.8
MS.gene062966.t1	AT2G45210	39.189	74	41	2	15	85	74	146	6.91e-13	60.5
MS.gene062966.t1	AT4G00880	40.698	86	46	3	3	86	13	95	7.79e-13	59.7
MS.gene062966.t1	AT3G60690	42.647	68	35	2	20	84	88	154	1.50e-12	59.7
MS.gene062966.t1	AT3G12830	41.791	67	38	1	11	77	41	106	2.54e-12	58.5
MS.gene062966.t1	AT5G53590	42.029	69	34	2	19	82	45	112	2.19e-11	56.2
MS.gene062966.t1	AT5G03310	46.269	67	31	2	19	80	38	104	2.41e-11	55.5
MS.gene062966.t1	AT2G36210	38.235	68	42	0	20	87	54	121	3.04e-11	55.8
MS.gene062966.t1	AT1G56150	42.254	71	39	2	11	80	28	97	3.09e-11	55.1
MS.gene062966.t1	AT1G16510	44.828	58	31	1	20	77	54	110	4.80e-11	55.5

Find 22 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGTTGATTCAAGTATGATAT+TGG	0.237365	4.2:-35486415	None:intergenic
TATACTGCTAGATATCCTTT+TGG	0.332278	4.2:-35486361	None:intergenic
GTTGATTCAAGTATGATATT+GGG	0.382183	4.2:-35486414	None:intergenic
TTTGAATATGATCATCCAAT+GGG	0.517345	4.2:+35486475	MS.gene062966:CDS
ATTCAAATTGCTCCTCAGCT+TGG	0.518679	4.2:-35486459	None:intergenic
AGGATATCTAGCAGTATATG+TGG	0.532379	4.2:+35486366	MS.gene062966:CDS
AGAAGGACATCATCTTCAAA+AGG	0.536345	4.2:+35486328	MS.gene062966:CDS
AAAGGTGTGGATGTTCCAAA+AGG	0.538636	4.2:+35486346	MS.gene062966:CDS
GTTTACCTGGTATCCTCAGA+AGG	0.541030	4.2:+35486311	MS.gene062966:CDS
GATGTCCTTCTGAGGATACC+AGG	0.552721	4.2:-35486316	None:intergenic
ATATCAATTCTCGTTTGAGT+AGG	0.554567	4.2:+35486536	MS.gene062966:CDS
GACATCATCTTCAAAAGGTG+TGG	0.556023	4.2:+35486333	MS.gene062966:CDS
ATTTGAATATGATCATCCAA+TGG	0.556875	4.2:+35486474	MS.gene062966:CDS
AAGAAAATGTCTTCTCTGCA+AGG	0.560690	4.2:-35486511	None:intergenic
TCTAGCAGTATATGTGGGAG+AGG	0.571467	4.2:+35486372	MS.gene062966:CDS
ATGTGGGAGAGGAAATGAAA+CGG	0.576130	4.2:+35486383	MS.gene062966:CDS
TTGAAGATGATGTCCTTCTG+AGG	0.597296	4.2:-35486324	None:intergenic
TTCAACAAGTCTTGAAACGA+TGG	0.599919	4.2:-35486436	None:intergenic
GGAATAGTGAGACCACCCAT+TGG	0.603922	4.2:-35486490	None:intergenic
GGATATCTAGCAGTATATGT+GGG	0.608430	4.2:+35486367	MS.gene062966:CDS
GAATATGATCATCCAATGGG+TGG	0.646056	4.2:+35486478	MS.gene062966:CDS
AGACTTGTTGAACCAAGCTG+AGG	0.647765	4.2:+35486447	MS.gene062966:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATTTGAATATGATCATCCAA+TGG	+	chr4.2:35486474-35486493	MS.gene062966:CDS	25.0%
!	TTTGAATATGATCATCCAAT+GGG	+	chr4.2:35486475-35486494	MS.gene062966:CDS	25.0%
!!	GTTGATTCAAGTATGATATT+GGG	-	chr4.2:35486417-35486436	None:intergenic	25.0%
	ATATCAATTCTCGTTTGAGT+AGG	+	chr4.2:35486536-35486555	MS.gene062966:CDS	30.0%
!	GGTTGATTCAAGTATGATAT+TGG	-	chr4.2:35486418-35486437	None:intergenic	30.0%
!	TATACTGCTAGATATCCTTT+TGG	-	chr4.2:35486364-35486383	None:intergenic	30.0%
	AAGAAAATGTCTTCTCTGCA+AGG	-	chr4.2:35486514-35486533	None:intergenic	35.0%
	AGAAGGACATCATCTTCAAA+AGG	+	chr4.2:35486328-35486347	MS.gene062966:CDS	35.0%
	AGGATATCTAGCAGTATATG+TGG	+	chr4.2:35486366-35486385	MS.gene062966:CDS	35.0%
	GGATATCTAGCAGTATATGT+GGG	+	chr4.2:35486367-35486386	MS.gene062966:CDS	35.0%
	TTCAACAAGTCTTGAAACGA+TGG	-	chr4.2:35486439-35486458	None:intergenic	35.0%
	ATGTGGGAGAGGAAATGAAA+CGG	+	chr4.2:35486383-35486402	MS.gene062966:CDS	40.0%
	ATTCAAATTGCTCCTCAGCT+TGG	-	chr4.2:35486462-35486481	None:intergenic	40.0%
	GAATATGATCATCCAATGGG+TGG	+	chr4.2:35486478-35486497	MS.gene062966:CDS	40.0%
	GACATCATCTTCAAAAGGTG+TGG	+	chr4.2:35486333-35486352	MS.gene062966:CDS	40.0%
	TTGAAGATGATGTCCTTCTG+AGG	-	chr4.2:35486327-35486346	None:intergenic	40.0%
!	AAAGGTGTGGATGTTCCAAA+AGG	+	chr4.2:35486346-35486365	MS.gene062966:CDS	40.0%
	GTTTACCTGGTATCCTCAGA+AGG	+	chr4.2:35486311-35486330	MS.gene062966:CDS	45.0%
	TCTAGCAGTATATGTGGGAG+AGG	+	chr4.2:35486372-35486391	MS.gene062966:CDS	45.0%
!!	AGACTTGTTGAACCAAGCTG+AGG	+	chr4.2:35486447-35486466	MS.gene062966:CDS	45.0%
	GGAATAGTGAGACCACCCAT+TGG	-	chr4.2:35486493-35486512	None:intergenic	50.0%
!	GATGTCCTTCTGAGGATACC+AGG	-	chr4.2:35486319-35486338	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	35486301	35486564	35486301	ID=MS.gene062966
chr4.2	mRNA	35486301	35486564	35486301	ID=MS.gene062966.t1;Parent=MS.gene062966
chr4.2	exon	35486301	35486564	35486301	ID=MS.gene062966.t1.exon1;Parent=MS.gene062966.t1
chr4.2	CDS	35486301	35486564	35486301	ID=cds.MS.gene062966.t1;Parent=MS.gene062966.t1

Gene Sequence	>MS.gene062966 ATGGGTTTTCGTTTACCTGGTATCCTCAGAAGGACATCATCTTCAAAAGGTGTGGATGTTCCAAAAGGATATCTAGCAGTATATGTGGGAGAGGAAATGAAACGGTTTGTGATCCCAATATCATACTTGAATCAACCATCGTTTCAAGACTTGTTGAACCAAGCTGAGGAGCAATTTGAATATGATCATCCAATGGGTGGTCTCACTATTCCTTGCAGAGAAGACATTTTCTTAGATATCAATTCTCGTTTGAGTAGGTGTTAA
Protein sequence	>MS.gene062966.t1 MGFRLPGILRRTSSSKGVDVPKGYLAVYVGEEMKRFVIPISYLNQPSFQDLLNQAEEQFEYDHPMGGLTIPCREDIFLDINSRLSRC