AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene34085


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34085.t1 MTR_1g094180 95.578 294 13 0 1 294 1 294 0.0 582
MS.gene34085.t1 MTR_1g094180 95.578 294 13 0 1 294 5 298 0.0 582
MS.gene34085.t1 MTR_1g050538 60.241 249 93 3 1 248 1 244 1.40e-101 297
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34085.t1 AT2G39170 42.231 251 105 4 1 248 1 214 1.07e-50 167

Find 48 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCTTTCTGTCTGAGAATT+TGG 0.214548 1.2:+74384259 None:intergenic
CATGAATTCTCTACCGTTGA+TGG 0.268118 1.2:-74384601 MS.gene34085:CDS
CGGCATAACTTCATCATTAC+AGG 0.308986 1.2:-74384041 MS.gene34085:CDS
ACCTATCATCTCATTGACTA+TGG 0.347470 1.2:+74384357 None:intergenic
AATGCTGATATCACTTTCAT+TGG 0.349970 1.2:+74383955 None:intergenic
TTGTCAGCTTTAATGACATT+TGG 0.384892 1.2:+74384475 None:intergenic
TCTTCAACCTGACTCGATAT+AGG 0.401910 1.2:+74383998 None:intergenic
AACGAGGAGAAGATAAATAC+TGG 0.403101 1.2:-74383614 MS.gene34085:CDS
GTGATTGACCGTGGCAAATC+AGG 0.414138 1.2:+74384070 None:intergenic
TCCATAGTCAATGAGATGAT+AGG 0.429467 1.2:-74384358 MS.gene34085:CDS
AAGATAAATACTGGAAATAG+TGG 0.432837 1.2:-74383605 MS.gene34085:CDS
AACTTTGCACACTGAAGACT+TGG 0.451922 1.2:-74384425 MS.gene34085:CDS
TTGGTGTTGATGTTGATGAA+TGG 0.456620 1.2:+74384278 None:intergenic
AGGTGACATAAACAAATCTC+TGG 0.458453 1.2:-74384338 MS.gene34085:CDS
AAACTAGAGGAGTGGCTTGA+AGG 0.458965 1.2:-74383878 MS.gene34085:CDS
AGTTGTTGTCATTGTCAGAA+AGG 0.459938 1.2:-74383931 MS.gene34085:CDS
TGATTTGCCTATATCGAGTC+AGG 0.466514 1.2:-74384005 MS.gene34085:CDS
ATATCGTAGCTTCTCTTCGT+TGG 0.471398 1.2:+74384093 None:intergenic
CTCGATATAGGCAAATCATC+AGG 0.503555 1.2:+74384010 None:intergenic
TTTGCACACTGAAGACTTGG+AGG 0.512330 1.2:-74384422 MS.gene34085:CDS
TCTACTGATCCTTTACCATC+AGG 0.516231 1.2:+74384118 None:intergenic
GTTGATGTTGATGAATGGAT+TGG 0.527813 1.2:+74384283 None:intergenic
TGGCAACAGCCTGATGGTAA+AGG 0.529712 1.2:-74384127 MS.gene34085:CDS
CGTGGCTAACGAACCATCTG+TGG 0.531543 1.2:-74384536 MS.gene34085:CDS
TATAGGCAAATCATCAGGTT+CGG 0.532495 1.2:+74384015 None:intergenic
ACATTCTTTCATTGATCGAA+CGG 0.535936 1.2:+74384387 None:intergenic
ACTGGAAATAGTGGATTCAA+AGG 0.551817 1.2:-74383596 MS.gene34085:CDS
GTTCGATCAATGAAAGAATG+TGG 0.571899 1.2:-74384385 MS.gene34085:CDS
TTTAAAGTCGGCGAAACAGA+AGG 0.572954 1.2:-74384164 MS.gene34085:CDS
GTGGCTAACGAACCATCTGT+GGG 0.577979 1.2:-74384535 MS.gene34085:CDS
TTCAAGTGGCAACAGCCTGA+TGG 0.588182 1.2:-74384133 MS.gene34085:CDS
TACGATATCCTGATTTGCCA+CGG 0.614233 1.2:-74384078 MS.gene34085:CDS
TAAGAAGCTTCTCGAGAACG+AGG 0.616733 1.2:-74383630 MS.gene34085:CDS
CCACGGTCAATCACATCAGA+CGG 0.616977 1.2:-74384061 MS.gene34085:CDS
TATCTCCACAAAACCATCAA+CGG 0.623173 1.2:+74384588 None:intergenic
AGAAGGTTAACAGTTTCAAG+TGG 0.626891 1.2:-74384147 MS.gene34085:CDS
TAATAAAGAAGCTAAACTAG+AGG 0.628039 1.2:-74383891 MS.gene34085:CDS
TGTGGAGATAAGTGAATGCA+TGG 0.634348 1.2:-74384575 MS.gene34085:CDS
CCGTGATGATTATTGCGACA+CGG 0.638583 1.2:-74383846 MS.gene34085:CDS
ATAAAGTAAAGCCCCACAGA+TGG 0.642500 1.2:+74384523 None:intergenic
GACACGGATGAAGAAGAAAG+AGG 0.646339 1.2:-74383830 MS.gene34085:intron
AAGAAGCTAAACTAGAGGAG+TGG 0.653782 1.2:-74383886 MS.gene34085:CDS
CCGTGTCGCAATAATCATCA+CGG 0.657697 1.2:+74383846 None:intergenic
GGCATAACTTCATCATTACA+GGG 0.684347 1.2:-74384040 MS.gene34085:CDS
CCGTCTGATGTGATTGACCG+TGG 0.714657 1.2:+74384061 None:intergenic
GGCGGATATGATCAAATACG+TGG 0.717411 1.2:-74384554 MS.gene34085:CDS
TGGCTAACGAACCATCTGTG+GGG 0.751780 1.2:-74384534 MS.gene34085:CDS
GGAGATAAGTGAATGCATGG+CGG 0.818548 1.2:-74384572 MS.gene34085:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAACAAAAAAACCAAAAAA+AGG - chr1.2:74383862-74383881 MS.gene34085:CDS 15.0%
!! AAACAAAAAAACCAAAAAAA+GGG - chr1.2:74383863-74383882 MS.gene34085:CDS 15.0%
!!! TTTTTCAAATGTTTTAAAGT+CGG - chr1.2:74383993-74384012 MS.gene34085:CDS 15.0%
! AAGATAAATACTGGAAATAG+TGG - chr1.2:74384564-74384583 MS.gene34085:CDS 25.0%
! TAATAAAGAAGCTAAACTAG+AGG - chr1.2:74384278-74384297 MS.gene34085:CDS 25.0%
! TGACAAAAATATTGTCGAAA+AGG - chr1.2:74383711-74383730 MS.gene34085:intron 25.0%
AATGCTGATATCACTTTCAT+TGG + chr1.2:74384217-74384236 None:intergenic 30.0%
ACATTCTTTCATTGATCGAA+CGG + chr1.2:74383785-74383804 None:intergenic 30.0%
! TTGTCAGCTTTAATGACATT+TGG + chr1.2:74383697-74383716 None:intergenic 30.0%
AACGAGGAGAAGATAAATAC+TGG - chr1.2:74384555-74384574 MS.gene34085:CDS 35.0%
AGAAGGTTAACAGTTTCAAG+TGG - chr1.2:74384022-74384041 MS.gene34085:CDS 35.0%
AGGTGACATAAACAAATCTC+TGG - chr1.2:74383831-74383850 MS.gene34085:CDS 35.0%
AGTTGTTGTCATTGTCAGAA+AGG - chr1.2:74384238-74384257 MS.gene34085:CDS 35.0%
GAACAAACATGATACGCTAT+TGG - chr1.2:74384447-74384466 MS.gene34085:CDS 35.0%
GAAGAAAGAGGTTAGTAGTT+TGG - chr1.2:74384351-74384370 MS.gene34085:CDS 35.0%
GGCATAACTTCATCATTACA+GGG - chr1.2:74384129-74384148 MS.gene34085:CDS 35.0%
GTTCGATCAATGAAAGAATG+TGG - chr1.2:74383784-74383803 MS.gene34085:intron 35.0%
GTTCTTTCTGTCTGAGAATT+TGG + chr1.2:74383913-74383932 None:intergenic 35.0%
TATAGGCAAATCATCAGGTT+CGG + chr1.2:74384157-74384176 None:intergenic 35.0%
TATCTCCACAAAACCATCAA+CGG + chr1.2:74383584-74383603 None:intergenic 35.0%
TCCATAGTCAATGAGATGAT+AGG - chr1.2:74383811-74383830 MS.gene34085:intron 35.0%
! ACCTATCATCTCATTGACTA+TGG + chr1.2:74383815-74383834 None:intergenic 35.0%
! AGACAGAAAGAACTCGTTTT+TGG - chr1.2:74383920-74383939 MS.gene34085:CDS 35.0%
! GTTGATGTTGATGAATGGAT+TGG + chr1.2:74383889-74383908 None:intergenic 35.0%
! GTTGATTTCCTTATGTCAAC+AGG - chr1.2:74384516-74384535 MS.gene34085:CDS 35.0%
!! ACAGAAAGAACTCGTTTTTG+GGG - chr1.2:74383922-74383941 MS.gene34085:CDS 35.0%
!! ACTGGAAATAGTGGATTCAA+AGG - chr1.2:74384573-74384592 MS.gene34085:CDS 35.0%
!! ATGCTTTTTATGCTCAGGAA+GGG - chr1.2:74383950-74383969 MS.gene34085:CDS 35.0%
!! CAATCATGCTTTTTATGCTC+AGG - chr1.2:74383945-74383964 MS.gene34085:CDS 35.0%
!! GACAGAAAGAACTCGTTTTT+GGG - chr1.2:74383921-74383940 MS.gene34085:CDS 35.0%
!! TTGGTGTTGATGTTGATGAA+TGG + chr1.2:74383894-74383913 None:intergenic 35.0%
AACTTTGCACACTGAAGACT+TGG - chr1.2:74383744-74383763 MS.gene34085:intron 40.0%
AAGAAGCTAAACTAGAGGAG+TGG - chr1.2:74384283-74384302 MS.gene34085:CDS 40.0%
ATAAAGTAAAGCCCCACAGA+TGG + chr1.2:74383649-74383668 None:intergenic 40.0%
ATATCGTAGCTTCTCTTCGT+TGG + chr1.2:74384079-74384098 None:intergenic 40.0%
CATGAATTCTCTACCGTTGA+TGG - chr1.2:74383568-74383587 MS.gene34085:CDS 40.0%
CGGCATAACTTCATCATTAC+AGG - chr1.2:74384128-74384147 MS.gene34085:CDS 40.0%
CTCGATATAGGCAAATCATC+AGG + chr1.2:74384162-74384181 None:intergenic 40.0%
GCTTCTTACCTGTTGACATA+AGG + chr1.2:74384527-74384546 None:intergenic 40.0%
TCTACTGATCCTTTACCATC+AGG + chr1.2:74384054-74384073 None:intergenic 40.0%
TGTGGAGATAAGTGAATGCA+TGG - chr1.2:74383594-74383613 MS.gene34085:CDS 40.0%
! CTGCATTTTGAGCATGTTGT+TGG + chr1.2:74383672-74383691 None:intergenic 40.0%
! TACGATATCCTGATTTGCCA+CGG - chr1.2:74384091-74384110 MS.gene34085:CDS 40.0%
! TCTTCAACCTGACTCGATAT+AGG + chr1.2:74384174-74384193 None:intergenic 40.0%
! TGATTTGCCTATATCGAGTC+AGG - chr1.2:74384164-74384183 MS.gene34085:CDS 40.0%
! TTTAAAGTCGGCGAAACAGA+AGG - chr1.2:74384005-74384024 MS.gene34085:CDS 40.0%
!! CATGCTTTTTATGCTCAGGA+AGG - chr1.2:74383949-74383968 MS.gene34085:CDS 40.0%
!! TGCTTTTTATGCTCAGGAAG+GGG - chr1.2:74383951-74383970 MS.gene34085:CDS 40.0%
!!! GAATGGATTGGCCCTTTTTT+TGG + chr1.2:74383877-74383896 None:intergenic 40.0%
AAACTAGAGGAGTGGCTTGA+AGG - chr1.2:74384291-74384310 MS.gene34085:CDS 45.0%
CCGTGATGATTATTGCGACA+CGG - chr1.2:74384323-74384342 MS.gene34085:CDS 45.0%
CCGTGTCGCAATAATCATCA+CGG + chr1.2:74384326-74384345 None:intergenic 45.0%
GACACGGATGAAGAAGAAAG+AGG - chr1.2:74384339-74384358 MS.gene34085:CDS 45.0%
GGAGATAAGTGAATGCATGG+CGG - chr1.2:74383597-74383616 MS.gene34085:CDS 45.0%
GGCGGATATGATCAAATACG+TGG - chr1.2:74383615-74383634 MS.gene34085:CDS 45.0%
TAAGAAGCTTCTCGAGAACG+AGG - chr1.2:74384539-74384558 MS.gene34085:CDS 45.0%
TTTGCACACTGAAGACTTGG+AGG - chr1.2:74383747-74383766 MS.gene34085:intron 45.0%
!!! CTCTACCGTTGATGGTTTTG+TGG - chr1.2:74383576-74383595 MS.gene34085:CDS 45.0%
CCACGGTCAATCACATCAGA+CGG - chr1.2:74384108-74384127 MS.gene34085:CDS 50.0%
GTGATTGACCGTGGCAAATC+AGG + chr1.2:74384102-74384121 None:intergenic 50.0%
GTGGCTAACGAACCATCTGT+GGG - chr1.2:74383634-74383653 MS.gene34085:CDS 50.0%
TGGCAACAGCCTGATGGTAA+AGG - chr1.2:74384042-74384061 MS.gene34085:CDS 50.0%
TGGCTAACGAACCATCTGTG+GGG - chr1.2:74383635-74383654 MS.gene34085:intron 50.0%
TTCAAGTGGCAACAGCCTGA+TGG - chr1.2:74384036-74384055 MS.gene34085:CDS 50.0%
CCGTCTGATGTGATTGACCG+TGG + chr1.2:74384111-74384130 None:intergenic 55.0%
CGTGGCTAACGAACCATCTG+TGG - chr1.2:74383633-74383652 MS.gene34085:CDS 55.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 74383565 74384626 74383565 ID=MS.gene34085
chr1.2 mRNA 74383565 74384626 74383565 ID=MS.gene34085.t1;Parent=MS.gene34085
chr1.2 exon 74383831 74384626 74383831 ID=MS.gene34085.t1.exon1;Parent=MS.gene34085.t1
chr1.2 CDS 74383831 74384626 74383831 ID=cds.MS.gene34085.t1;Parent=MS.gene34085.t1
chr1.2 exon 74383565 74383653 74383565 ID=MS.gene34085.t1.exon2;Parent=MS.gene34085.t1
chr1.2 CDS 74383565 74383653 74383565 ID=cds.MS.gene34085.t1;Parent=MS.gene34085.t1
Gene Sequence

>MS.gene34085

ATGCATGAATTCTCTACCGTTGATGGTTTTGTGGAGATAAGTGAATGCATGGCGGATATGATCAAATACGTGGCTAACGAACCATCTGTGGGGCTTTACTTTATCCAACAACATGCTCAAAATGCAGTGCCAAATGTCATTAAAGCTGACAAAAATATTGTCGAAAAGGTTCATGAAACAACTTTGCACACTGAAGACTTGGAGGACTCTATTACAGCCGTTCGATCAATGAAAGAATGTGGATTTTCCATAGTCAATGAGATGATAGGTGACATAAACAAATCTCTGGAAATTTTGAAAACAAAAAAACCAAAAAAAGGGCCAATCCATTCATCAACATCAACACCAAATTCTCAGACAGAAAGAACTCGTTTTTGGGGCAATCATGCTTTTTATGCTCAGGAAGGGGATGATAAAAAGAGTAACTATTTTTCAAATGTTTTAAAGTCGGCGAAACAGAAGGTTAACAGTTTCAAGTGGCAACAGCCTGATGGTAAAGGATCAGTAGATTCCAACGAAGAGAAGCTACGATATCCTGATTTGCCACGGTCAATCACATCAGACGGCATAACTTCATCATTACAGGGAACCGAACCTGATGATTTGCCTATATCGAGTCAGGTTGAAGACGAGTCTCAACACGAACAGACCAATGAAAGTGATATCAGCATTAAGTTGTTGTCATTGTCAGAAAGGTTTGATGACTTTAAAGCTAATAAAGAAGCTAAACTAGAGGAGTGGCTTGAAGGAACTAGCAACCGTGATGATTATTGCGACACGGATGAAGAAGAAAGAGGTTAGTAGTTTGGTTACACTTGACGAAAACTTCAGCGTACACATTCATCAAATGATCTGCTAGTGCTATGCTGTAATACATTCTTTGAACAAACATGATACGCTATTGGATTAATCAATGTATCGCACTGCATTCTTTTTTCGTGAAACTTCAATGTTGATTTCCTTATGTCAACAGGTAAGAAGCTTCTCGAGAACGAGGAGAAGATAAATACTGGAAATAGTGGATTCAAAGGTGAAGTACTATTGCATGCAGTTCAGCCCTGA

Protein sequence

>MS.gene34085.t1

MHEFSTVDGFVEISECMADMIKYVANEPSVGLYFIQQHAQNAVPNVIKADKNIVEKVHETTLHTEDLEDSITAVRSMKECGFSIVNEMIGDINKSLEILKTKKPKKGPIHSSTSTPNSQTERTRFWGNHAFYAQEGDDKKSNYFSNVLKSAKQKVNSFKWQQPDGKGSVDSNEEKLRYPDLPRSITSDGITSSLQGTEPDDLPISSQVEDESQHEQTNESDISIKLLSLSERFDDFKANKEAKLEEWLEGTSNRDDYCDTDEEERGKKLLENEEKINTGNSGFKGEVLLHAVQP