Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41681.t1 | XP_003624559.1 | 98.6 | 436 | 5 | 1 | 1 | 436 | 1 | 435 | 1.40E-244 | 855.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41681.t1 | P35614 | 91.3 | 436 | 37 | 1 | 1 | 436 | 1 | 435 | 5.7e-231 | 801.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41681.t1 | G7KYC6 | 98.6 | 436 | 5 | 1 | 1 | 436 | 1 | 435 | 1.0e-244 | 855.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04920 | MS.gene41681 | 0.803667 | 3.13E-49 | -1.69E-46 |
| MS.gene049283 | MS.gene41681 | 0.838796 | 2.32E-57 | -1.69E-46 |
| MS.gene049368 | MS.gene41681 | 0.801371 | 9.29E-49 | -1.69E-46 |
| MS.gene049983 | MS.gene41681 | 0.809022 | 2.32E-50 | -1.69E-46 |
| MS.gene050084 | MS.gene41681 | 0.809258 | 2.07E-50 | -1.69E-46 |
| MS.gene050085 | MS.gene41681 | 0.8257 | 4.06E-54 | -1.69E-46 |
| MS.gene050114 | MS.gene41681 | 0.830381 | 3.03E-55 | -1.69E-46 |
| MS.gene050359 | MS.gene41681 | 0.809099 | 2.24E-50 | -1.69E-46 |
| MS.gene050556 | MS.gene41681 | 0.811637 | 6.34E-51 | -1.69E-46 |
| MS.gene050646 | MS.gene41681 | 0.803377 | 3.59E-49 | -1.69E-46 |
| MS.gene050654 | MS.gene41681 | 0.825849 | 3.75E-54 | -1.69E-46 |
| MS.gene050689 | MS.gene41681 | 0.801392 | 9.20E-49 | -1.69E-46 |
| MS.gene050752 | MS.gene41681 | 0.806589 | 7.64E-50 | -1.69E-46 |
| MS.gene050838 | MS.gene41681 | 0.871072 | 9.03E-67 | -1.69E-46 |
| MS.gene050945 | MS.gene41681 | 0.86502 | 7.99E-65 | -1.69E-46 |
| MS.gene051003 | MS.gene41681 | 0.83365 | 4.72E-56 | -1.69E-46 |
| MS.gene051233 | MS.gene41681 | 0.820616 | 6.24E-53 | -1.69E-46 |
| MS.gene051234 | MS.gene41681 | 0.844388 | 7.80E-59 | -1.69E-46 |
| MS.gene051343 | MS.gene41681 | 0.87861 | 2.44E-69 | -1.69E-46 |
| MS.gene051676 | MS.gene41681 | 0.828135 | 1.06E-54 | -1.69E-46 |
| MS.gene051699 | MS.gene41681 | 0.80505 | 1.61E-49 | -1.69E-46 |
| MS.gene05188 | MS.gene41681 | 0.847497 | 1.11E-59 | -1.69E-46 |
| MS.gene051902 | MS.gene41681 | 0.8419 | 3.59E-58 | -1.69E-46 |
| MS.gene051975 | MS.gene41681 | 0.884262 | 2.23E-71 | -1.69E-46 |
| MS.gene052072 | MS.gene41681 | 0.821782 | 3.36E-53 | -1.69E-46 |
| MS.gene052242 | MS.gene41681 | -0.824695 | 7.02E-54 | -1.69E-46 |
| MS.gene052307 | MS.gene41681 | 0.827016 | 1.97E-54 | -1.69E-46 |
| MS.gene052335 | MS.gene41681 | 0.808056 | 3.74E-50 | -1.69E-46 |
| MS.gene052342 | MS.gene41681 | 0.815044 | 1.13E-51 | -1.69E-46 |
| MS.gene052434 | MS.gene41681 | 0.802301 | 5.99E-49 | -1.69E-46 |
| MS.gene052533 | MS.gene41681 | 0.811134 | 8.15E-51 | -1.69E-46 |
| MS.gene052609 | MS.gene41681 | 0.822757 | 2.00E-53 | -1.69E-46 |
| MS.gene052932 | MS.gene41681 | 0.824737 | 6.86E-54 | -1.69E-46 |
| MS.gene052947 | MS.gene41681 | 0.802314 | 5.95E-49 | -1.69E-46 |
| MS.gene053058 | MS.gene41681 | 0.867242 | 1.58E-65 | -1.69E-46 |
| MS.gene053079 | MS.gene41681 | 0.810035 | 1.41E-50 | -1.69E-46 |
| MS.gene053143 | MS.gene41681 | 0.806177 | 9.34E-50 | -1.69E-46 |
| MS.gene053298 | MS.gene41681 | 0.810716 | 1.00E-50 | -1.69E-46 |
| MS.gene053564 | MS.gene41681 | 0.84946 | 3.19E-60 | -1.69E-46 |
| MS.gene053786 | MS.gene41681 | 0.828002 | 1.15E-54 | -1.69E-46 |
| MS.gene053963 | MS.gene41681 | 0.800704 | 1.27E-48 | -1.69E-46 |
| MS.gene054222 | MS.gene41681 | 0.82833 | 9.55E-55 | -1.69E-46 |
| MS.gene054403 | MS.gene41681 | 0.808709 | 2.71E-50 | -1.69E-46 |
| MS.gene054413 | MS.gene41681 | 0.809982 | 1.45E-50 | -1.69E-46 |
| MS.gene054576 | MS.gene41681 | 0.807312 | 5.38E-50 | -1.69E-46 |
| MS.gene05469 | MS.gene41681 | 0.808897 | 2.47E-50 | -1.69E-46 |
| MS.gene054892 | MS.gene41681 | 0.82248 | 2.32E-53 | -1.69E-46 |
| MS.gene054893 | MS.gene41681 | 0.830391 | 3.01E-55 | -1.69E-46 |
| MS.gene055311 | MS.gene41681 | 0.83151 | 1.60E-55 | -1.69E-46 |
| MS.gene055313 | MS.gene41681 | 0.835103 | 2.04E-56 | -1.69E-46 |
| MS.gene055314 | MS.gene41681 | 0.827729 | 1.33E-54 | -1.69E-46 |
| MS.gene055513 | MS.gene41681 | 0.841578 | 4.36E-58 | -1.69E-46 |
| MS.gene055603 | MS.gene41681 | 0.805656 | 1.20E-49 | -1.69E-46 |
| MS.gene055864 | MS.gene41681 | 0.803031 | 4.23E-49 | -1.69E-46 |
| MS.gene056045 | MS.gene41681 | 0.836058 | 1.17E-56 | -1.69E-46 |
| MS.gene056097 | MS.gene41681 | 0.830963 | 2.18E-55 | -1.69E-46 |
| MS.gene056181 | MS.gene41681 | 0.836324 | 1.00E-56 | -1.69E-46 |
| MS.gene056390 | MS.gene41681 | 0.80954 | 1.80E-50 | -1.69E-46 |
| MS.gene056403 | MS.gene41681 | 0.843007 | 1.83E-58 | -1.69E-46 |
| MS.gene056509 | MS.gene41681 | 0.809469 | 1.86E-50 | -1.69E-46 |
| MS.gene056605 | MS.gene41681 | 0.833767 | 4.41E-56 | -1.69E-46 |
| MS.gene056699 | MS.gene41681 | 0.806084 | 9.77E-50 | -1.69E-46 |
| MS.gene056787 | MS.gene41681 | 0.821934 | 3.10E-53 | -1.69E-46 |
| MS.gene05689 | MS.gene41681 | 0.83419 | 3.46E-56 | -1.69E-46 |
| MS.gene056986 | MS.gene41681 | 0.857829 | 1.25E-62 | -1.69E-46 |
| MS.gene057102 | MS.gene41681 | 0.825151 | 5.48E-54 | -1.69E-46 |
| MS.gene05781 | MS.gene41681 | 0.805141 | 1.54E-49 | -1.69E-46 |
| MS.gene057890 | MS.gene41681 | -0.83739 | 5.35E-57 | -1.69E-46 |
| MS.gene057999 | MS.gene41681 | 0.81162 | 6.39E-51 | -1.69E-46 |
| MS.gene058074 | MS.gene41681 | 0.805012 | 1.64E-49 | -1.69E-46 |
| MS.gene058159 | MS.gene41681 | 0.807972 | 3.89E-50 | -1.69E-46 |
| MS.gene058261 | MS.gene41681 | 0.813537 | 2.43E-51 | -1.69E-46 |
| MS.gene058292 | MS.gene41681 | 0.81399 | 1.94E-51 | -1.69E-46 |
| MS.gene058638 | MS.gene41681 | 0.803235 | 3.84E-49 | -1.69E-46 |
| MS.gene058639 | MS.gene41681 | 0.812414 | 4.29E-51 | -1.69E-46 |
| MS.gene058758 | MS.gene41681 | 0.812244 | 4.67E-51 | -1.69E-46 |
| MS.gene059007 | MS.gene41681 | 0.81251 | 4.09E-51 | -1.69E-46 |
| MS.gene059146 | MS.gene41681 | 0.857239 | 1.87E-62 | -1.69E-46 |
| MS.gene059170 | MS.gene41681 | 0.830999 | 2.14E-55 | -1.69E-46 |
| MS.gene059171 | MS.gene41681 | 0.815628 | 8.39E-52 | -1.69E-46 |
| MS.gene059227 | MS.gene41681 | 0.802536 | 5.36E-49 | -1.69E-46 |
| MS.gene05985 | MS.gene41681 | -0.843463 | 1.38E-58 | -1.69E-46 |
| MS.gene059965 | MS.gene41681 | 0.811928 | 5.48E-51 | -1.69E-46 |
| MS.gene06010 | MS.gene41681 | 0.816778 | 4.64E-52 | -1.69E-46 |
| MS.gene06011 | MS.gene41681 | 0.834206 | 3.43E-56 | -1.69E-46 |
| MS.gene060365 | MS.gene41681 | 0.834424 | 3.02E-56 | -1.69E-46 |
| MS.gene060763 | MS.gene41681 | 0.806524 | 7.89E-50 | -1.69E-46 |
| MS.gene060933 | MS.gene41681 | 0.841276 | 5.24E-58 | -1.69E-46 |
| MS.gene060935 | MS.gene41681 | 0.802979 | 4.34E-49 | -1.69E-46 |
| MS.gene060937 | MS.gene41681 | 0.823643 | 1.24E-53 | -1.69E-46 |
| MS.gene061006 | MS.gene41681 | 0.812049 | 5.15E-51 | -1.69E-46 |
| MS.gene061100 | MS.gene41681 | 0.847786 | 9.28E-60 | -1.69E-46 |
| MS.gene061160 | MS.gene41681 | 0.809008 | 2.34E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene41681 | MS.gene52281 | PPI |
| MS.gene41681 | MS.gene51920 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41681.t1 | MTR_7g084860 | 98.624 | 436 | 5 | 1 | 1 | 436 | 1 | 435 | 0.0 | 883 |
| MS.gene41681.t1 | MTR_6g478120 | 89.885 | 435 | 43 | 1 | 1 | 434 | 1 | 435 | 0.0 | 820 |
| MS.gene41681.t1 | MTR_4g103720 | 85.082 | 429 | 63 | 1 | 1 | 428 | 1 | 429 | 0.0 | 769 |
| MS.gene41681.t1 | MTR_6g478090 | 65.306 | 245 | 63 | 1 | 190 | 434 | 4 | 226 | 2.36e-103 | 306 |
| MS.gene41681.t1 | MTR_4g103730 | 79.618 | 157 | 29 | 1 | 130 | 286 | 6 | 159 | 2.25e-84 | 256 |
| MS.gene41681.t1 | MTR_6g081000 | 62.745 | 153 | 56 | 1 | 131 | 283 | 4 | 155 | 1.29e-58 | 189 |
| MS.gene41681.t1 | MTR_7g109660 | 73.529 | 68 | 15 | 2 | 34 | 99 | 16 | 82 | 1.04e-23 | 95.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41681.t1 | AT3G26618 | 91.284 | 436 | 37 | 1 | 1 | 436 | 1 | 435 | 0.0 | 826 |
| MS.gene41681.t1 | AT1G12920 | 90.509 | 432 | 40 | 1 | 5 | 436 | 4 | 434 | 0.0 | 818 |
| MS.gene41681.t1 | AT5G47880 | 86.480 | 429 | 54 | 1 | 5 | 429 | 6 | 434 | 0.0 | 776 |
| MS.gene41681.t1 | AT5G47880 | 86.480 | 429 | 54 | 1 | 5 | 429 | 6 | 434 | 0.0 | 776 |
Find 87 sgRNAs with CRISPR-Local
Find 103 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTCATTGTATGGGAGAATT+TGG | 0.171668 | 7.1:+20133917 | MS.gene41681:CDS |
| TGCAGAGGGTTTGGTGGTAT+TGG | 0.226379 | 7.1:+20134167 | MS.gene41681:CDS |
| CCTCCTCTACCATGTTTCTT+TGG | 0.261663 | 7.1:-20133483 | None:intergenic |
| GTCTCGTATGGTGGGGAAAA+TGG | 0.297905 | 7.1:+20133726 | MS.gene41681:CDS |
| AAGATGTTGGGAGATGAATT+TGG | 0.306796 | 7.1:+20133102 | MS.gene41681:CDS |
| TGCGGCCACATCTGCAGATT+TGG | 0.309390 | 7.1:+20134037 | MS.gene41681:CDS |
| CGACCCTTCTTGAGATTTGT+TGG | 0.312643 | 7.1:-20134138 | None:intergenic |
| CTAGAATCTGATGACAAGTT+TGG | 0.315461 | 7.1:+20133372 | MS.gene41681:CDS |
| GTGAATGAGTACAAAAGATT+TGG | 0.348119 | 7.1:+20134095 | MS.gene41681:CDS |
| AATCCAAATATGCACTTAAT+CGG | 0.359874 | 7.1:-20134264 | None:intergenic |
| CACATTGAAGGCTCTAGAAA+TGG | 0.360512 | 7.1:+20133881 | MS.gene41681:CDS |
| ATCTGCAGATTTGGAGGTTC+AGG | 0.366347 | 7.1:+20134046 | MS.gene41681:CDS |
| TGGCTCCAAGCACTGATTGC+CGG | 0.392694 | 7.1:-20133155 | None:intergenic |
| ATGTGGCCGCATCGCGGAAG+TGG | 0.405903 | 7.1:-20134025 | None:intergenic |
| AGAGGGTTTGGTGGTATTGG+AGG | 0.406600 | 7.1:+20134170 | MS.gene41681:CDS |
| GAGACTGGTAAATATGTTTA+CGG | 0.407711 | 7.1:+20133849 | MS.gene41681:CDS |
| AGATAAAGTGATGCATTGAT+TGG | 0.409138 | 7.1:-20133309 | None:intergenic |
| CGGTTGAAACACTCATTGTA+TGG | 0.412463 | 7.1:+20133907 | MS.gene41681:CDS |
| TTTATTGTTATGGATGGAAA+TGG | 0.413603 | 7.1:+20133396 | MS.gene41681:CDS |
| ATTCAAGAGAAACGCTTGAT+AGG | 0.417924 | 7.1:+20133801 | MS.gene41681:CDS |
| TTCCACTTAATGTCCCGAAA+AGG | 0.426411 | 7.1:-20133422 | None:intergenic |
| TCCACTTAATGTCCCGAAAA+GGG | 0.426867 | 7.1:-20133421 | None:intergenic |
| TGTAATAAAGCATTTGAAGA+AGG | 0.432701 | 7.1:+20133983 | MS.gene41681:CDS |
| CAGACAAGAATATTGAGATA+TGG | 0.434895 | 7.1:+20132977 | MS.gene41681:CDS |
| ACTAGTCAGCCTAATGTTGC+TGG | 0.435213 | 7.1:+20133606 | MS.gene41681:CDS |
| TAATGTTGCTGGACTCATAC+TGG | 0.438513 | 7.1:+20133617 | MS.gene41681:CDS |
| AAACATATTTACCAGTCTCT+TGG | 0.438527 | 7.1:-20133845 | None:intergenic |
| CTGGAAGCTGCAAGAGGTAA+TGG | 0.449382 | 7.1:+20133024 | MS.gene41681:CDS |
| ACATCAAGAGTAGGGTAAAC+CGG | 0.459780 | 7.1:+20133136 | MS.gene41681:CDS |
| GTATAAAGAACCAATCCATT+TGG | 0.460218 | 7.1:-20133222 | None:intergenic |
| AGTATGAGTCCAGCAACATT+AGG | 0.468191 | 7.1:-20133615 | None:intergenic |
| TATAACAAGGTTCCTCCAAA+TGG | 0.471094 | 7.1:+20133207 | MS.gene41681:CDS |
| ATCTCGTGTCACTAAGATGT+TGG | 0.473352 | 7.1:+20133089 | MS.gene41681:CDS |
| CGGAGTTGATGACACATTGA+AGG | 0.474084 | 7.1:+20133869 | MS.gene41681:CDS |
| CAAGGTTCCTCCAAATGGAT+TGG | 0.477410 | 7.1:+20133212 | MS.gene41681:CDS |
| CTTGCCGTCCTCAGTGAGAA+TGG | 0.478058 | 7.1:-20133250 | None:intergenic |
| AATGGATTGGTTCTTTATAC+CGG | 0.484935 | 7.1:+20133225 | MS.gene41681:CDS |
| TCTCGTGTCACTAAGATGTT+GGG | 0.490357 | 7.1:+20133090 | MS.gene41681:CDS |
| ACATTAGGCTGACTAGTAGC+AGG | 0.499450 | 7.1:-20133600 | None:intergenic |
| CAAAAGATTTGGATGCTCGC+TGG | 0.500903 | 7.1:+20134106 | MS.gene41681:CDS |
| ACATTGAAGGCTCTAGAAAT+GGG | 0.514674 | 7.1:+20133882 | MS.gene41681:CDS |
| GGGAGAATTTGGATATTACT+AGG | 0.521356 | 7.1:+20133928 | MS.gene41681:CDS |
| GTAATAAAGCATTTGAAGAA+GGG | 0.522658 | 7.1:+20133984 | MS.gene41681:CDS |
| CCTCTGTTGAGCAGAAGTAA+TGG | 0.522824 | 7.1:-20133175 | None:intergenic |
| GGCCACATCTGCAGATTTGG+AGG | 0.524825 | 7.1:+20134040 | MS.gene41681:CDS |
| GTAAACCGGCAATCAGTGCT+TGG | 0.540063 | 7.1:+20133150 | MS.gene41681:CDS |
| CTGCTTCAAACATCAAGAGT+AGG | 0.540306 | 7.1:+20133127 | MS.gene41681:CDS |
| TTCACAAACCACTCAAGTAA+TGG | 0.546965 | 7.1:-20134077 | None:intergenic |
| GAAGAGATAAGCCAAGAGAC+TGG | 0.550060 | 7.1:+20133834 | MS.gene41681:CDS |
| TGAGGACGGCAAGGAAAAGA+AGG | 0.553356 | 7.1:+20133260 | MS.gene41681:CDS |
| GTGGTTGATGTCTCGTATGG+TGG | 0.555643 | 7.1:+20133717 | MS.gene41681:CDS |
| AACCTCCAAATCTGCAGATG+TGG | 0.562932 | 7.1:-20134042 | None:intergenic |
| AGAAGATGCCATTACTTGAG+TGG | 0.565327 | 7.1:+20134069 | MS.gene41681:CDS |
| GATGCATTGATTGGTTTGAA+AGG | 0.571596 | 7.1:-20133300 | None:intergenic |
| GTGACCAACAAATCTCAAGA+AGG | 0.573488 | 7.1:+20134134 | MS.gene41681:CDS |
| TGCTTCAAACATCAAGAGTA+GGG | 0.575365 | 7.1:+20133128 | MS.gene41681:CDS |
| TAAAGAACCAATCCATTTGG+AGG | 0.576280 | 7.1:-20133219 | None:intergenic |
| GTCCTCAGTGAGAATGGTAC+CGG | 0.581083 | 7.1:-20133244 | None:intergenic |
| CGAATGATCTGATATCAAGC+TGG | 0.590096 | 7.1:-20134205 | None:intergenic |
| GATGAGTTATCTGATGATGA+TGG | 0.590510 | 7.1:+20134227 | MS.gene41681:CDS |
| TGGTTGATGTCTCGTATGGT+GGG | 0.591547 | 7.1:+20133718 | MS.gene41681:CDS |
| CTACCAAAGAAACATGGTAG+AGG | 0.592428 | 7.1:+20133480 | MS.gene41681:CDS |
| ATTCTTGTCTGATTCACCAT+CGG | 0.594219 | 7.1:-20132965 | None:intergenic |
| GTTGCTGGACTCATACTGGC+TGG | 0.598808 | 7.1:+20133621 | MS.gene41681:CDS |
| CATTCTCACTGAGGACGGCA+AGG | 0.604123 | 7.1:+20133251 | MS.gene41681:CDS |
| GGTTGAAACACTCATTGTAT+GGG | 0.621105 | 7.1:+20133908 | MS.gene41681:CDS |
| AACGTGGTTGATGTCTCGTA+TGG | 0.621452 | 7.1:+20133714 | MS.gene41681:CDS |
| CCAAAGAAACATGGTAGAGG+AGG | 0.625338 | 7.1:+20133483 | MS.gene41681:CDS |
| TGACCAACAAATCTCAAGAA+GGG | 0.625562 | 7.1:+20134135 | MS.gene41681:CDS |
| CCATTACTTCTGCTCAACAG+AGG | 0.649740 | 7.1:+20133175 | MS.gene41681:CDS |
| GGTACCATTCTCACTGAGGA+CGG | 0.651299 | 7.1:+20133246 | MS.gene41681:CDS |
| GAAGGCTCTAGAAATGGGTG+CGG | 0.653960 | 7.1:+20133887 | MS.gene41681:CDS |
| ACACGAGATATTTGATCACG+TGG | 0.654304 | 7.1:-20133075 | None:intergenic |
| CATTAGGCTGACTAGTAGCA+GGG | 0.656179 | 7.1:-20133599 | None:intergenic |
| AAAGGACTGGAAGCTGCAAG+AGG | 0.664338 | 7.1:+20133018 | MS.gene41681:CDS |
| CTTTATTACAATCTCACCAG+TGG | 0.665088 | 7.1:-20133970 | None:intergenic |
| TCAAAGCCACTTCCGCGATG+CGG | 0.668435 | 7.1:+20134019 | MS.gene41681:CDS |
| GAGGTTGAAACTATATAACA+AGG | 0.687653 | 7.1:+20133194 | MS.gene41681:CDS |
| AGGGTTGATGTAAAACTGAG+TGG | 0.688267 | 7.1:-20133580 | None:intergenic |
| CTGCAGATGTGGCCGCATCG+CGG | 0.688811 | 7.1:-20134031 | None:intergenic |
| TCTGCATAAACAATGGCCGA+TGG | 0.701549 | 7.1:+20132949 | None:intergenic |
| GGTTGATGTCTCGTATGGTG+GGG | 0.707328 | 7.1:+20133719 | MS.gene41681:CDS |
| CGAGATATTTGATCACGTGG+AGG | 0.714871 | 7.1:-20133072 | None:intergenic |
| GTTGATCTACCAAAGAAACA+TGG | 0.720655 | 7.1:+20133474 | MS.gene41681:CDS |
| ATTAAGTGGAAATACTCGCG+AGG | 0.721816 | 7.1:+20133434 | MS.gene41681:CDS |
| TGATGATGATGGAGTGTACG+AGG | 0.728062 | 7.1:+20134238 | MS.gene41681:CDS |
| TACCGGTACCATTCTCACTG+AGG | 0.737721 | 7.1:+20133242 | MS.gene41681:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTTATTGTTATGGATGGAAA+TGG | + | chr7.1:20133396-20133415 | MS.gene41681:CDS | 25.0% |
| !! | AAGATCAAAAAGTTGATCAA+AGG | + | chr7.1:20133000-20133019 | MS.gene41681:CDS | 25.0% |
| !! | GTAATAAAGCATTTGAAGAA+GGG | + | chr7.1:20133984-20134003 | MS.gene41681:CDS | 25.0% |
| !! | TGTAATAAAGCATTTGAAGA+AGG | + | chr7.1:20133983-20134002 | MS.gene41681:CDS | 25.0% |
| !!! | CAAGTTTGGTTTTATTGTTA+TGG | + | chr7.1:20133386-20133405 | MS.gene41681:CDS | 25.0% |
| !!! | TTTGGTTTTATTGTTATGGA+TGG | + | chr7.1:20133390-20133409 | MS.gene41681:CDS | 25.0% |
| AAACATATTTACCAGTCTCT+TGG | - | chr7.1:20133848-20133867 | None:intergenic | 30.0% | |
| CAGACAAGAATATTGAGATA+TGG | + | chr7.1:20132977-20132996 | MS.gene41681:CDS | 30.0% | |
| GAGACTGGTAAATATGTTTA+CGG | + | chr7.1:20133849-20133868 | MS.gene41681:CDS | 30.0% | |
| GAGGTTGAAACTATATAACA+AGG | + | chr7.1:20133194-20133213 | MS.gene41681:CDS | 30.0% | |
| GTATAAAGAACCAATCCATT+TGG | - | chr7.1:20133225-20133244 | None:intergenic | 30.0% | |
| GTGAATGAGTACAAAAGATT+TGG | + | chr7.1:20134095-20134114 | MS.gene41681:CDS | 30.0% | |
| ! | AGATAAAGTGATGCATTGAT+TGG | - | chr7.1:20133312-20133331 | None:intergenic | 30.0% |
| !! | AATATTTTTGCTTGCAAACG+AGG | - | chr7.1:20133693-20133712 | None:intergenic | 30.0% |
| !! | AATGGATTGGTTCTTTATAC+CGG | + | chr7.1:20133225-20133244 | MS.gene41681:CDS | 30.0% |
| AAAAACGACACAATTACGTC+AGG | + | chr7.1:20133541-20133560 | MS.gene41681:CDS | 35.0% | |
| AAGATGTTGGGAGATGAATT+TGG | + | chr7.1:20133102-20133121 | MS.gene41681:CDS | 35.0% | |
| ACTCATTGTATGGGAGAATT+TGG | + | chr7.1:20133917-20133936 | MS.gene41681:CDS | 35.0% | |
| ATTCAAGAGAAACGCTTGAT+AGG | + | chr7.1:20133801-20133820 | MS.gene41681:CDS | 35.0% | |
| ATTCTTGTCTGATTCACCAT+CGG | - | chr7.1:20132968-20132987 | None:intergenic | 35.0% | |
| CTAGAATCTGATGACAAGTT+TGG | + | chr7.1:20133372-20133391 | MS.gene41681:CDS | 35.0% | |
| CTTTATTACAATCTCACCAG+TGG | - | chr7.1:20133973-20133992 | None:intergenic | 35.0% | |
| GCAAGCAAAAATATTGAACG+TGG | + | chr7.1:20133698-20133717 | MS.gene41681:CDS | 35.0% | |
| GGGAGAATTTGGATATTACT+AGG | + | chr7.1:20133928-20133947 | MS.gene41681:CDS | 35.0% | |
| GGTTGAAACACTCATTGTAT+GGG | + | chr7.1:20133908-20133927 | MS.gene41681:CDS | 35.0% | |
| GTTGATCTACCAAAGAAACA+TGG | + | chr7.1:20133474-20133493 | MS.gene41681:CDS | 35.0% | |
| TAAAGAACCAATCCATTTGG+AGG | - | chr7.1:20133222-20133241 | None:intergenic | 35.0% | |
| TATAACAAGGTTCCTCCAAA+TGG | + | chr7.1:20133207-20133226 | MS.gene41681:CDS | 35.0% | |
| TGACCAACAAATCTCAAGAA+GGG | + | chr7.1:20134135-20134154 | MS.gene41681:CDS | 35.0% | |
| TGCTTCAAACATCAAGAGTA+GGG | + | chr7.1:20133128-20133147 | MS.gene41681:CDS | 35.0% | |
| TTCACAAACCACTCAAGTAA+TGG | - | chr7.1:20134080-20134099 | None:intergenic | 35.0% | |
| ! | CAAAAAGTTGATCAAAGGAC+TGG | + | chr7.1:20133005-20133024 | MS.gene41681:CDS | 35.0% |
| ! | GATGAGTTATCTGATGATGA+TGG | + | chr7.1:20134227-20134246 | MS.gene41681:CDS | 35.0% |
| !! | ACATTGAAGGCTCTAGAAAT+GGG | + | chr7.1:20133882-20133901 | MS.gene41681:CDS | 35.0% |
| !! | GATGCATTGATTGGTTTGAA+AGG | - | chr7.1:20133303-20133322 | None:intergenic | 35.0% |
| !!! | AATTGTGTCGTTTTTCCATC+CGG | - | chr7.1:20133536-20133555 | None:intergenic | 35.0% |
| ACACGAGATATTTGATCACG+TGG | - | chr7.1:20133078-20133097 | None:intergenic | 40.0% | |
| ACATCAAGAGTAGGGTAAAC+CGG | + | chr7.1:20133136-20133155 | MS.gene41681:CDS | 40.0% | |
| AGAAGATGCCATTACTTGAG+TGG | + | chr7.1:20134069-20134088 | MS.gene41681:CDS | 40.0% | |
| AGTATGAGTCCAGCAACATT+AGG | - | chr7.1:20133618-20133637 | None:intergenic | 40.0% | |
| ATTAAGTGGAAATACTCGCG+AGG | + | chr7.1:20133434-20133453 | MS.gene41681:CDS | 40.0% | |
| CGAATGATCTGATATCAAGC+TGG | - | chr7.1:20134208-20134227 | None:intergenic | 40.0% | |
| CGGTTGAAACACTCATTGTA+TGG | + | chr7.1:20133907-20133926 | MS.gene41681:CDS | 40.0% | |
| CTACCAAAGAAACATGGTAG+AGG | + | chr7.1:20133480-20133499 | MS.gene41681:CDS | 40.0% | |
| CTGCTTCAAACATCAAGAGT+AGG | + | chr7.1:20133127-20133146 | MS.gene41681:CDS | 40.0% | |
| GTGACCAACAAATCTCAAGA+AGG | + | chr7.1:20134134-20134153 | MS.gene41681:CDS | 40.0% | |
| TAATGTTGCTGGACTCATAC+TGG | + | chr7.1:20133617-20133636 | MS.gene41681:CDS | 40.0% | |
| TCCACTTAATGTCCCGAAAA+GGG | - | chr7.1:20133424-20133443 | None:intergenic | 40.0% | |
| TTCCACTTAATGTCCCGAAA+AGG | - | chr7.1:20133425-20133444 | None:intergenic | 40.0% | |
| ! | ATCTCGTGTCACTAAGATGT+TGG | + | chr7.1:20133089-20133108 | MS.gene41681:CDS | 40.0% |
| ! | ATGGAAATGGAACCCTTTTC+GGG | + | chr7.1:20133409-20133428 | MS.gene41681:CDS | 40.0% |
| ! | GATGGAAATGGAACCCTTTT+CGG | + | chr7.1:20133408-20133427 | MS.gene41681:CDS | 40.0% |
| ! | TCTCGTGTCACTAAGATGTT+GGG | + | chr7.1:20133090-20133109 | MS.gene41681:CDS | 40.0% |
| !! | AGGGTTGATGTAAAACTGAG+TGG | - | chr7.1:20133583-20133602 | None:intergenic | 40.0% |
| !! | CACATTGAAGGCTCTAGAAA+TGG | + | chr7.1:20133881-20133900 | MS.gene41681:CDS | 40.0% |
| AACCTCCAAATCTGCAGATG+TGG | - | chr7.1:20134045-20134064 | None:intergenic | 45.0% | |
| ACATTAGGCTGACTAGTAGC+AGG | - | chr7.1:20133603-20133622 | None:intergenic | 45.0% | |
| ACTAGTCAGCCTAATGTTGC+TGG | + | chr7.1:20133606-20133625 | MS.gene41681:CDS | 45.0% | |
| ATCTGCAGATTTGGAGGTTC+AGG | + | chr7.1:20134046-20134065 | MS.gene41681:CDS | 45.0% | |
| CAAAAGATTTGGATGCTCGC+TGG | + | chr7.1:20134106-20134125 | MS.gene41681:CDS | 45.0% | |
| CAAGGTTCCTCCAAATGGAT+TGG | + | chr7.1:20133212-20133231 | MS.gene41681:CDS | 45.0% | |
| CATTAGGCTGACTAGTAGCA+GGG | - | chr7.1:20133602-20133621 | None:intergenic | 45.0% | |
| CCAAAGAAACATGGTAGAGG+AGG | + | chr7.1:20133483-20133502 | MS.gene41681:CDS | 45.0% | |
| CCATTACTTCTGCTCAACAG+AGG | + | chr7.1:20133175-20133194 | MS.gene41681:CDS | 45.0% | |
| CCTCCTCTACCATGTTTCTT+TGG | - | chr7.1:20133486-20133505 | None:intergenic | 45.0% | |
| CCTCTGTTGAGCAGAAGTAA+TGG | - | chr7.1:20133178-20133197 | None:intergenic | 45.0% | |
| CGACCCTTCTTGAGATTTGT+TGG | - | chr7.1:20134141-20134160 | None:intergenic | 45.0% | |
| CGAGATATTTGATCACGTGG+AGG | - | chr7.1:20133075-20133094 | None:intergenic | 45.0% | |
| GAAGAGATAAGCCAAGAGAC+TGG | + | chr7.1:20133834-20133853 | MS.gene41681:CDS | 45.0% | |
| GTGCTGAAAAATGCTACCAC+TGG | + | chr7.1:20133954-20133973 | MS.gene41681:CDS | 45.0% | |
| ! | AACGTGGTTGATGTCTCGTA+TGG | + | chr7.1:20133714-20133733 | MS.gene41681:CDS | 45.0% |
| ! | TGGTTGATGTCTCGTATGGT+GGG | + | chr7.1:20133718-20133737 | MS.gene41681:CDS | 45.0% |
| !! | ACCCTTTTCGGGACATTAAG+TGG | + | chr7.1:20133420-20133439 | MS.gene41681:CDS | 45.0% |
| !! | CGGAGTTGATGACACATTGA+AGG | + | chr7.1:20133869-20133888 | MS.gene41681:CDS | 45.0% |
| !! | TGATGATGATGGAGTGTACG+AGG | + | chr7.1:20134238-20134257 | MS.gene41681:CDS | 45.0% |
| AAAGGACTGGAAGCTGCAAG+AGG | + | chr7.1:20133018-20133037 | MS.gene41681:CDS | 50.0% | |
| CTGGAAGCTGCAAGAGGTAA+TGG | + | chr7.1:20133024-20133043 | MS.gene41681:CDS | 50.0% | |
| GGTACCATTCTCACTGAGGA+CGG | + | chr7.1:20133246-20133265 | MS.gene41681:CDS | 50.0% | |
| GTAAACCGGCAATCAGTGCT+TGG | + | chr7.1:20133150-20133169 | MS.gene41681:CDS | 50.0% | |
| GTCCTCAGTGAGAATGGTAC+CGG | - | chr7.1:20133247-20133266 | None:intergenic | 50.0% | |
| TACCGGTACCATTCTCACTG+AGG | + | chr7.1:20133242-20133261 | MS.gene41681:CDS | 50.0% | |
| TGAGGACGGCAAGGAAAAGA+AGG | + | chr7.1:20133260-20133279 | MS.gene41681:CDS | 50.0% | |
| ! | AAGGGTCGCAGTTTTGCAGA+GGG | + | chr7.1:20134153-20134172 | MS.gene41681:CDS | 50.0% |
| ! | CAGTTTTGCAGAGGGTTTGG+TGG | + | chr7.1:20134161-20134180 | MS.gene41681:CDS | 50.0% |
| ! | CTTTGCGTTTTGCTCGTCTC+CGG | + | chr7.1:20133514-20133533 | MS.gene41681:CDS | 50.0% |
| ! | GGTTGATGTCTCGTATGGTG+GGG | + | chr7.1:20133719-20133738 | MS.gene41681:CDS | 50.0% |
| ! | GTCTCGTATGGTGGGGAAAA+TGG | + | chr7.1:20133726-20133745 | MS.gene41681:CDS | 50.0% |
| ! | GTGGTTGATGTCTCGTATGG+TGG | + | chr7.1:20133717-20133736 | MS.gene41681:CDS | 50.0% |
| ! | TCGCAGTTTTGCAGAGGGTT+TGG | + | chr7.1:20134158-20134177 | MS.gene41681:CDS | 50.0% |
| !! | AGAGGGTTTGGTGGTATTGG+AGG | + | chr7.1:20134170-20134189 | MS.gene41681:CDS | 50.0% |
| !! | GAAGGCTCTAGAAATGGGTG+CGG | + | chr7.1:20133887-20133906 | MS.gene41681:CDS | 50.0% |
| !! | TGCAGAGGGTTTGGTGGTAT+TGG | + | chr7.1:20134167-20134186 | MS.gene41681:CDS | 50.0% |
| CATTCTCACTGAGGACGGCA+AGG | + | chr7.1:20133251-20133270 | MS.gene41681:CDS | 55.0% | |
| CTTGCCGTCCTCAGTGAGAA+TGG | - | chr7.1:20133253-20133272 | None:intergenic | 55.0% | |
| GGCCACATCTGCAGATTTGG+AGG | + | chr7.1:20134040-20134059 | MS.gene41681:CDS | 55.0% | |
| GTTGCTGGACTCATACTGGC+TGG | + | chr7.1:20133621-20133640 | MS.gene41681:CDS | 55.0% | |
| TCAAAGCCACTTCCGCGATG+CGG | + | chr7.1:20134019-20134038 | MS.gene41681:CDS | 55.0% | |
| TGCGGCCACATCTGCAGATT+TGG | + | chr7.1:20134037-20134056 | MS.gene41681:CDS | 55.0% | |
| ! | GAAGGGTCGCAGTTTTGCAG+AGG | + | chr7.1:20134152-20134171 | MS.gene41681:CDS | 55.0% |
| !! | TGGCTCCAAGCACTGATTGC+CGG | - | chr7.1:20133158-20133177 | None:intergenic | 55.0% |
| ! | GCGTTTTGCTCGTCTCCGGA+TGG | + | chr7.1:20133518-20133537 | MS.gene41681:CDS | 60.0% |
| ATGTGGCCGCATCGCGGAAG+TGG | - | chr7.1:20134028-20134047 | None:intergenic | 65.0% | |
| CTGCAGATGTGGCCGCATCG+CGG | - | chr7.1:20134034-20134053 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 20132961 | 20134271 | 20132961 | ID=MS.gene41681 |
| chr7.1 | mRNA | 20132961 | 20134271 | 20132961 | ID=MS.gene41681.t1;Parent=MS.gene41681 |
| chr7.1 | exon | 20132961 | 20134271 | 20132961 | ID=MS.gene41681.t1.exon1;Parent=MS.gene41681.t1 |
| chr7.1 | CDS | 20132961 | 20134271 | 20132961 | ID=cds.MS.gene41681.t1;Parent=MS.gene41681.t1 |
| Gene Sequence |
| Protein sequence |
