Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52281.t1 | XP_003624559.1 | 98.9 | 436 | 4 | 1 | 1 | 436 | 1 | 435 | 6.20E-245 | 856.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52281.t1 | P35614 | 91.5 | 436 | 36 | 1 | 1 | 436 | 1 | 435 | 2.6e-231 | 802.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52281.t1 | G7KYC6 | 98.9 | 436 | 4 | 1 | 1 | 436 | 1 | 435 | 4.5e-245 | 856.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049166 | MS.gene52281 | 0.836927 | 7.02E-57 | -1.69E-46 |
| MS.gene049756 | MS.gene52281 | 0.801271 | 9.74E-49 | -1.69E-46 |
| MS.gene049861 | MS.gene52281 | 0.80692 | 6.51E-50 | -1.69E-46 |
| MS.gene050085 | MS.gene52281 | 0.832424 | 9.53E-56 | -1.69E-46 |
| MS.gene050114 | MS.gene52281 | 0.85665 | 2.79E-62 | -1.69E-46 |
| MS.gene050277 | MS.gene52281 | 0.821499 | 3.91E-53 | -1.69E-46 |
| MS.gene050288 | MS.gene52281 | 0.805614 | 1.23E-49 | -1.69E-46 |
| MS.gene050317 | MS.gene52281 | 0.840475 | 8.50E-58 | -1.69E-46 |
| MS.gene050320 | MS.gene52281 | 0.84194 | 3.50E-58 | -1.69E-46 |
| MS.gene050689 | MS.gene52281 | 0.818165 | 2.26E-52 | -1.69E-46 |
| MS.gene050752 | MS.gene52281 | 0.862698 | 4.22E-64 | -1.69E-46 |
| MS.gene050838 | MS.gene52281 | 0.909322 | 6.48E-82 | -1.69E-46 |
| MS.gene050945 | MS.gene52281 | 0.812076 | 5.08E-51 | -1.69E-46 |
| MS.gene050955 | MS.gene52281 | 0.82884 | 7.19E-55 | -1.69E-46 |
| MS.gene051003 | MS.gene52281 | 0.883651 | 3.74E-71 | -1.69E-46 |
| MS.gene051233 | MS.gene52281 | 0.836139 | 1.11E-56 | -1.69E-46 |
| MS.gene051234 | MS.gene52281 | 0.853616 | 2.12E-61 | -1.69E-46 |
| MS.gene051245 | MS.gene52281 | 0.818547 | 1.85E-52 | -1.69E-46 |
| MS.gene051343 | MS.gene52281 | 0.872914 | 2.20E-67 | -1.69E-46 |
| MS.gene051676 | MS.gene52281 | 0.80108 | 1.07E-48 | -1.69E-46 |
| MS.gene051746 | MS.gene52281 | 0.843923 | 1.04E-58 | -1.69E-46 |
| MS.gene051748 | MS.gene52281 | 0.860425 | 2.08E-63 | -1.69E-46 |
| MS.gene051756 | MS.gene52281 | 0.857826 | 1.25E-62 | -1.69E-46 |
| MS.gene051757 | MS.gene52281 | 0.812036 | 5.19E-51 | -1.69E-46 |
| MS.gene051850 | MS.gene52281 | 0.82675 | 2.29E-54 | -1.69E-46 |
| MS.gene05188 | MS.gene52281 | 0.86394 | 1.74E-64 | -1.69E-46 |
| MS.gene051902 | MS.gene52281 | 0.820634 | 6.18E-53 | -1.69E-46 |
| MS.gene051903 | MS.gene52281 | 0.856601 | 2.88E-62 | -1.69E-46 |
| MS.gene051975 | MS.gene52281 | 0.885459 | 7.99E-72 | -1.69E-46 |
| MS.gene052072 | MS.gene52281 | 0.815468 | 9.10E-52 | -1.69E-46 |
| MS.gene052335 | MS.gene52281 | 0.808624 | 2.83E-50 | -1.69E-46 |
| MS.gene052342 | MS.gene52281 | 0.814337 | 1.62E-51 | -1.69E-46 |
| MS.gene052434 | MS.gene52281 | 0.847482 | 1.12E-59 | -1.69E-46 |
| MS.gene052947 | MS.gene52281 | 0.837709 | 4.43E-57 | -1.69E-46 |
| MS.gene053058 | MS.gene52281 | 0.890795 | 7.13E-74 | -1.69E-46 |
| MS.gene053109 | MS.gene52281 | 0.85711 | 2.04E-62 | -1.69E-46 |
| MS.gene053298 | MS.gene52281 | 0.809239 | 2.09E-50 | -1.69E-46 |
| MS.gene053381 | MS.gene52281 | 0.826675 | 2.38E-54 | -1.69E-46 |
| MS.gene053564 | MS.gene52281 | 0.841955 | 3.47E-58 | -1.69E-46 |
| MS.gene053786 | MS.gene52281 | 0.825462 | 4.63E-54 | -1.69E-46 |
| MS.gene053952 | MS.gene52281 | 0.840584 | 7.96E-58 | -1.69E-46 |
| MS.gene054403 | MS.gene52281 | 0.860234 | 2.38E-63 | -1.69E-46 |
| MS.gene05469 | MS.gene52281 | 0.828724 | 7.67E-55 | -1.69E-46 |
| MS.gene05517 | MS.gene52281 | 0.803588 | 3.25E-49 | -1.69E-46 |
| MS.gene055311 | MS.gene52281 | 0.840735 | 7.27E-58 | -1.69E-46 |
| MS.gene055313 | MS.gene52281 | 0.819006 | 1.46E-52 | -1.69E-46 |
| MS.gene055314 | MS.gene52281 | 0.832292 | 1.03E-55 | -1.69E-46 |
| MS.gene055424 | MS.gene52281 | 0.831501 | 1.61E-55 | -1.69E-46 |
| MS.gene055513 | MS.gene52281 | 0.811342 | 7.34E-51 | -1.69E-46 |
| MS.gene055603 | MS.gene52281 | 0.839629 | 1.41E-57 | -1.69E-46 |
| MS.gene056097 | MS.gene52281 | 0.852042 | 5.99E-61 | -1.69E-46 |
| MS.gene056181 | MS.gene52281 | 0.843007 | 1.83E-58 | -1.69E-46 |
| MS.gene056403 | MS.gene52281 | 0.843917 | 1.04E-58 | -1.69E-46 |
| MS.gene056580 | MS.gene52281 | 0.81667 | 4.91E-52 | -1.69E-46 |
| MS.gene056605 | MS.gene52281 | 0.83984 | 1.25E-57 | -1.69E-46 |
| MS.gene056631 | MS.gene52281 | 0.821836 | 3.27E-53 | -1.69E-46 |
| MS.gene056752 | MS.gene52281 | 0.812198 | 4.78E-51 | -1.69E-46 |
| MS.gene056787 | MS.gene52281 | 0.862848 | 3.79E-64 | -1.69E-46 |
| MS.gene056986 | MS.gene52281 | 0.88975 | 1.83E-73 | -1.69E-46 |
| MS.gene056992 | MS.gene52281 | 0.826937 | 2.06E-54 | -1.69E-46 |
| MS.gene057057 | MS.gene52281 | 0.807802 | 4.23E-50 | -1.69E-46 |
| MS.gene057094 | MS.gene52281 | 0.891286 | 4.56E-74 | -1.69E-46 |
| MS.gene057144 | MS.gene52281 | 0.814447 | 1.53E-51 | -1.69E-46 |
| MS.gene057145 | MS.gene52281 | 0.817524 | 3.15E-52 | -1.69E-46 |
| MS.gene057234 | MS.gene52281 | 0.83169 | 1.45E-55 | -1.69E-46 |
| MS.gene057452 | MS.gene52281 | -0.813666 | 2.28E-51 | -1.69E-46 |
| MS.gene057705 | MS.gene52281 | 0.800731 | 1.26E-48 | -1.69E-46 |
| MS.gene05781 | MS.gene52281 | 0.831784 | 1.37E-55 | -1.69E-46 |
| MS.gene057890 | MS.gene52281 | -0.820637 | 6.17E-53 | -1.69E-46 |
| MS.gene057999 | MS.gene52281 | 0.861678 | 8.66E-64 | -1.69E-46 |
| MS.gene058054 | MS.gene52281 | 0.804251 | 2.36E-49 | -1.69E-46 |
| MS.gene058055 | MS.gene52281 | 0.806932 | 6.47E-50 | -1.69E-46 |
| MS.gene058074 | MS.gene52281 | 0.834709 | 2.56E-56 | -1.69E-46 |
| MS.gene058238 | MS.gene52281 | 0.861256 | 1.17E-63 | -1.69E-46 |
| MS.gene058261 | MS.gene52281 | 0.804919 | 1.71E-49 | -1.69E-46 |
| MS.gene058639 | MS.gene52281 | 0.810569 | 1.08E-50 | -1.69E-46 |
| MS.gene058646 | MS.gene52281 | 0.806552 | 7.78E-50 | -1.69E-46 |
| MS.gene058654 | MS.gene52281 | 0.832196 | 1.08E-55 | -1.69E-46 |
| MS.gene058758 | MS.gene52281 | 0.849961 | 2.31E-60 | -1.69E-46 |
| MS.gene058906 | MS.gene52281 | 0.804435 | 2.16E-49 | -1.69E-46 |
| MS.gene059146 | MS.gene52281 | 0.864606 | 1.08E-64 | -1.69E-46 |
| MS.gene059170 | MS.gene52281 | 0.808715 | 2.70E-50 | -1.69E-46 |
| MS.gene059171 | MS.gene52281 | 0.840574 | 8.01E-58 | -1.69E-46 |
| MS.gene059360 | MS.gene52281 | 0.817584 | 3.06E-52 | -1.69E-46 |
| MS.gene06001 | MS.gene52281 | 0.84573 | 3.38E-59 | -1.69E-46 |
| MS.gene06010 | MS.gene52281 | 0.835093 | 2.05E-56 | -1.69E-46 |
| MS.gene06011 | MS.gene52281 | 0.889673 | 1.96E-73 | -1.69E-46 |
| MS.gene060296 | MS.gene52281 | 0.806433 | 8.25E-50 | -1.69E-46 |
| MS.gene060465 | MS.gene52281 | 0.807275 | 5.47E-50 | -1.69E-46 |
| MS.gene060702 | MS.gene52281 | 0.80192 | 7.17E-49 | -1.69E-46 |
| MS.gene060763 | MS.gene52281 | 0.84568 | 3.49E-59 | -1.69E-46 |
| MS.gene060869 | MS.gene52281 | 0.825484 | 4.57E-54 | -1.69E-46 |
| MS.gene060870 | MS.gene52281 | 0.803168 | 3.96E-49 | -1.69E-46 |
| MS.gene061006 | MS.gene52281 | 0.80131 | 9.57E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene41681 | MS.gene52281 | PPI |
| MS.gene51920 | MS.gene52281 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52281.t1 | MTR_7g084860 | 98.853 | 436 | 4 | 1 | 1 | 436 | 1 | 435 | 0.0 | 885 |
| MS.gene52281.t1 | MTR_6g478120 | 90.115 | 435 | 42 | 1 | 1 | 434 | 1 | 435 | 0.0 | 822 |
| MS.gene52281.t1 | MTR_4g103720 | 85.315 | 429 | 62 | 1 | 1 | 428 | 1 | 429 | 0.0 | 770 |
| MS.gene52281.t1 | MTR_6g478090 | 65.714 | 245 | 62 | 1 | 190 | 434 | 4 | 226 | 5.38e-104 | 308 |
| MS.gene52281.t1 | MTR_4g103730 | 79.618 | 157 | 29 | 1 | 130 | 286 | 6 | 159 | 2.02e-84 | 256 |
| MS.gene52281.t1 | MTR_6g081000 | 62.745 | 153 | 56 | 1 | 131 | 283 | 4 | 155 | 1.27e-58 | 189 |
| MS.gene52281.t1 | MTR_7g109660 | 73.529 | 68 | 15 | 2 | 34 | 99 | 16 | 82 | 1.04e-23 | 95.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52281.t1 | AT3G26618 | 91.514 | 436 | 36 | 1 | 1 | 436 | 1 | 435 | 0.0 | 828 |
| MS.gene52281.t1 | AT1G12920 | 90.741 | 432 | 39 | 1 | 5 | 436 | 4 | 434 | 0.0 | 820 |
| MS.gene52281.t1 | AT5G47880 | 86.713 | 429 | 53 | 1 | 5 | 429 | 6 | 434 | 0.0 | 779 |
| MS.gene52281.t1 | AT5G47880 | 86.713 | 429 | 53 | 1 | 5 | 429 | 6 | 434 | 0.0 | 779 |
Find 87 sgRNAs with CRISPR-Local
Find 103 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTCATTGTATGGGAGAATT+TGG | 0.171668 | 7.3:+23761741 | MS.gene52281:CDS |
| TGCAGAGGGTTTGGTGGTAT+TGG | 0.226379 | 7.3:+23761991 | MS.gene52281:CDS |
| CCTCCTCTACCATGTTTCTT+TGG | 0.261663 | 7.3:-23761307 | None:intergenic |
| GTCTCGTATGGTGGGGAAAA+TGG | 0.297905 | 7.3:+23761550 | MS.gene52281:CDS |
| GCGAATGAGTACAAAAGATT+TGG | 0.305651 | 7.3:+23761919 | MS.gene52281:CDS |
| AAGATGTTGGGAGATGAATT+TGG | 0.306796 | 7.3:+23760926 | MS.gene52281:CDS |
| TGCGGCCACATCTGCAGATT+TGG | 0.309390 | 7.3:+23761861 | MS.gene52281:CDS |
| CGACCCTTCTTGAGATTTGT+TGG | 0.312643 | 7.3:-23761962 | None:intergenic |
| CTAGAATCTGATGACAAGTT+TGG | 0.315461 | 7.3:+23761196 | MS.gene52281:CDS |
| AATCCAAATATGCACTTAAT+CGG | 0.359874 | 7.3:-23762088 | None:intergenic |
| CACATTGAAGGCTCTAGAAA+TGG | 0.360512 | 7.3:+23761705 | MS.gene52281:CDS |
| ATCTGCAGATTTGGAGGTTC+AGG | 0.366347 | 7.3:+23761870 | MS.gene52281:CDS |
| TGGCTCCAAGCACTGATTGC+CGG | 0.392694 | 7.3:-23760979 | None:intergenic |
| ATGTGGCCGCATCGCGGAAG+TGG | 0.405903 | 7.3:-23761849 | None:intergenic |
| AGAGGGTTTGGTGGTATTGG+AGG | 0.406600 | 7.3:+23761994 | MS.gene52281:CDS |
| GAGACTGGTAAATATGTTTA+CGG | 0.407711 | 7.3:+23761673 | MS.gene52281:CDS |
| AGATAAAGTGATGCATTGAT+TGG | 0.409138 | 7.3:-23761133 | None:intergenic |
| CGGTTGAAACACTCATTGTA+TGG | 0.412463 | 7.3:+23761731 | MS.gene52281:CDS |
| TTTATTGTTATGGATGGAAA+TGG | 0.413603 | 7.3:+23761220 | MS.gene52281:CDS |
| ATTCAAGAGAAACGCTTGAT+AGG | 0.417924 | 7.3:+23761625 | MS.gene52281:CDS |
| TTCCACTTAATGTCCCGAAA+AGG | 0.426411 | 7.3:-23761246 | None:intergenic |
| TCCACTTAATGTCCCGAAAA+GGG | 0.426867 | 7.3:-23761245 | None:intergenic |
| TGTAATAAAGCATTTGAAGA+AGG | 0.432701 | 7.3:+23761807 | MS.gene52281:CDS |
| CAGACAAGAATATTGAGATA+TGG | 0.434895 | 7.3:+23760801 | MS.gene52281:CDS |
| ACTAGTCAGCCTAATGTTGC+TGG | 0.435213 | 7.3:+23761430 | MS.gene52281:CDS |
| TAATGTTGCTGGACTCATAC+TGG | 0.438513 | 7.3:+23761441 | MS.gene52281:CDS |
| AAACATATTTACCAGTCTCT+TGG | 0.438527 | 7.3:-23761669 | None:intergenic |
| CTGGAAGCTGCAAGAGGTAA+TGG | 0.449382 | 7.3:+23760848 | MS.gene52281:CDS |
| ACATCAAGAGTAGGGTAAAC+CGG | 0.459780 | 7.3:+23760960 | MS.gene52281:CDS |
| GTATAAAGAACCAATCCATT+TGG | 0.460218 | 7.3:-23761046 | None:intergenic |
| AGTATGAGTCCAGCAACATT+AGG | 0.468191 | 7.3:-23761439 | None:intergenic |
| TATAACAAGGTTCCTCCAAA+TGG | 0.471094 | 7.3:+23761031 | MS.gene52281:CDS |
| ATCTCGTGTCACTAAGATGT+TGG | 0.473352 | 7.3:+23760913 | MS.gene52281:CDS |
| CGGAGTTGATGACACATTGA+AGG | 0.474084 | 7.3:+23761693 | MS.gene52281:CDS |
| CAAGGTTCCTCCAAATGGAT+TGG | 0.477410 | 7.3:+23761036 | MS.gene52281:CDS |
| CTTGCCGTCCTCAGTGAGAA+TGG | 0.478058 | 7.3:-23761074 | None:intergenic |
| AATGGATTGGTTCTTTATAC+CGG | 0.484935 | 7.3:+23761049 | MS.gene52281:CDS |
| TCTCGTGTCACTAAGATGTT+GGG | 0.490357 | 7.3:+23760914 | MS.gene52281:CDS |
| ACATTAGGCTGACTAGTAGC+AGG | 0.499450 | 7.3:-23761424 | None:intergenic |
| CAAAAGATTTGGATGCTCGC+TGG | 0.500903 | 7.3:+23761930 | MS.gene52281:CDS |
| ACATTGAAGGCTCTAGAAAT+GGG | 0.514674 | 7.3:+23761706 | MS.gene52281:CDS |
| GGGAGAATTTGGATATTACT+AGG | 0.521356 | 7.3:+23761752 | MS.gene52281:CDS |
| GTAATAAAGCATTTGAAGAA+GGG | 0.522658 | 7.3:+23761808 | MS.gene52281:CDS |
| CCTCTGTTGAGCAGAAGTAA+TGG | 0.522824 | 7.3:-23760999 | None:intergenic |
| GGCCACATCTGCAGATTTGG+AGG | 0.524825 | 7.3:+23761864 | MS.gene52281:CDS |
| GTAAACCGGCAATCAGTGCT+TGG | 0.540063 | 7.3:+23760974 | MS.gene52281:CDS |
| CTGCTTCAAACATCAAGAGT+AGG | 0.540306 | 7.3:+23760951 | MS.gene52281:CDS |
| TTCGCAAACCACTCAAGTAA+TGG | 0.547585 | 7.3:-23761901 | None:intergenic |
| GAAGAGATAAGCCAAGAGAC+TGG | 0.550060 | 7.3:+23761658 | MS.gene52281:CDS |
| TGAGGACGGCAAGGAAAAGA+AGG | 0.553356 | 7.3:+23761084 | MS.gene52281:CDS |
| GTGGTTGATGTCTCGTATGG+TGG | 0.555643 | 7.3:+23761541 | MS.gene52281:CDS |
| AACCTCCAAATCTGCAGATG+TGG | 0.562932 | 7.3:-23761866 | None:intergenic |
| AGAAGATGCCATTACTTGAG+TGG | 0.565327 | 7.3:+23761893 | MS.gene52281:CDS |
| GATGCATTGATTGGTTTGAA+AGG | 0.571596 | 7.3:-23761124 | None:intergenic |
| GTGACCAACAAATCTCAAGA+AGG | 0.573488 | 7.3:+23761958 | MS.gene52281:CDS |
| TGCTTCAAACATCAAGAGTA+GGG | 0.575365 | 7.3:+23760952 | MS.gene52281:CDS |
| TAAAGAACCAATCCATTTGG+AGG | 0.576280 | 7.3:-23761043 | None:intergenic |
| GTCCTCAGTGAGAATGGTAC+CGG | 0.581083 | 7.3:-23761068 | None:intergenic |
| CGAATGATCTGATATCAAGC+TGG | 0.590096 | 7.3:-23762029 | None:intergenic |
| GATGAGTTATCTGATGATGA+TGG | 0.590510 | 7.3:+23762051 | MS.gene52281:CDS |
| TGGTTGATGTCTCGTATGGT+GGG | 0.591547 | 7.3:+23761542 | MS.gene52281:CDS |
| CTACCAAAGAAACATGGTAG+AGG | 0.592428 | 7.3:+23761304 | MS.gene52281:CDS |
| ATTCTTGTCTGATTCACCAT+CGG | 0.594219 | 7.3:-23760789 | None:intergenic |
| GTTGCTGGACTCATACTGGC+TGG | 0.598808 | 7.3:+23761445 | MS.gene52281:CDS |
| CATTCTCACTGAGGACGGCA+AGG | 0.604123 | 7.3:+23761075 | MS.gene52281:CDS |
| GGTTGAAACACTCATTGTAT+GGG | 0.621105 | 7.3:+23761732 | MS.gene52281:CDS |
| AACGTGGTTGATGTCTCGTA+TGG | 0.621452 | 7.3:+23761538 | MS.gene52281:CDS |
| CCAAAGAAACATGGTAGAGG+AGG | 0.625338 | 7.3:+23761307 | MS.gene52281:CDS |
| TGACCAACAAATCTCAAGAA+GGG | 0.625562 | 7.3:+23761959 | MS.gene52281:CDS |
| CCATTACTTCTGCTCAACAG+AGG | 0.649740 | 7.3:+23760999 | MS.gene52281:CDS |
| GGTACCATTCTCACTGAGGA+CGG | 0.651299 | 7.3:+23761070 | MS.gene52281:CDS |
| GAAGGCTCTAGAAATGGGTG+CGG | 0.653960 | 7.3:+23761711 | MS.gene52281:CDS |
| ACACGAGATATTTGATCACG+TGG | 0.654304 | 7.3:-23760899 | None:intergenic |
| CATTAGGCTGACTAGTAGCA+GGG | 0.656179 | 7.3:-23761423 | None:intergenic |
| AAAGGACTGGAAGCTGCAAG+AGG | 0.664338 | 7.3:+23760842 | MS.gene52281:CDS |
| CTTTATTACAATCTCACCAG+TGG | 0.665088 | 7.3:-23761794 | None:intergenic |
| TCAAAGCCACTTCCGCGATG+CGG | 0.668435 | 7.3:+23761843 | MS.gene52281:CDS |
| GAGGTTGAAACTATATAACA+AGG | 0.687653 | 7.3:+23761018 | MS.gene52281:CDS |
| AGGGTTGATGTAAAACTGAG+TGG | 0.688267 | 7.3:-23761404 | None:intergenic |
| CTGCAGATGTGGCCGCATCG+CGG | 0.688811 | 7.3:-23761855 | None:intergenic |
| TCTGCATAAACAATGGCCGA+TGG | 0.701549 | 7.3:+23760773 | None:intergenic |
| GGTTGATGTCTCGTATGGTG+GGG | 0.707328 | 7.3:+23761543 | MS.gene52281:CDS |
| CGAGATATTTGATCACGTGG+AGG | 0.714871 | 7.3:-23760896 | None:intergenic |
| GTTGATCTACCAAAGAAACA+TGG | 0.720655 | 7.3:+23761298 | MS.gene52281:CDS |
| ATTAAGTGGAAATACTCGCG+AGG | 0.721816 | 7.3:+23761258 | MS.gene52281:CDS |
| TGATGATGATGGAGTGTACG+AGG | 0.728062 | 7.3:+23762062 | MS.gene52281:CDS |
| TACCGGTACCATTCTCACTG+AGG | 0.737721 | 7.3:+23761066 | MS.gene52281:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTTATTGTTATGGATGGAAA+TGG | + | chr7.3:23761220-23761239 | MS.gene52281:CDS | 25.0% |
| !! | AAGATCAAAAAGTTGATCAA+AGG | + | chr7.3:23760824-23760843 | MS.gene52281:CDS | 25.0% |
| !! | GTAATAAAGCATTTGAAGAA+GGG | + | chr7.3:23761808-23761827 | MS.gene52281:CDS | 25.0% |
| !! | TGTAATAAAGCATTTGAAGA+AGG | + | chr7.3:23761807-23761826 | MS.gene52281:CDS | 25.0% |
| !!! | CAAGTTTGGTTTTATTGTTA+TGG | + | chr7.3:23761210-23761229 | MS.gene52281:CDS | 25.0% |
| !!! | TTTGGTTTTATTGTTATGGA+TGG | + | chr7.3:23761214-23761233 | MS.gene52281:CDS | 25.0% |
| AAACATATTTACCAGTCTCT+TGG | - | chr7.3:23761672-23761691 | None:intergenic | 30.0% | |
| CAGACAAGAATATTGAGATA+TGG | + | chr7.3:23760801-23760820 | MS.gene52281:CDS | 30.0% | |
| GAGACTGGTAAATATGTTTA+CGG | + | chr7.3:23761673-23761692 | MS.gene52281:CDS | 30.0% | |
| GAGGTTGAAACTATATAACA+AGG | + | chr7.3:23761018-23761037 | MS.gene52281:CDS | 30.0% | |
| GTATAAAGAACCAATCCATT+TGG | - | chr7.3:23761049-23761068 | None:intergenic | 30.0% | |
| ! | AGATAAAGTGATGCATTGAT+TGG | - | chr7.3:23761136-23761155 | None:intergenic | 30.0% |
| !! | AATATTTTTGCTTGCAAACG+AGG | - | chr7.3:23761517-23761536 | None:intergenic | 30.0% |
| !! | AATGGATTGGTTCTTTATAC+CGG | + | chr7.3:23761049-23761068 | MS.gene52281:CDS | 30.0% |
| AAAAACGACACAATTACGTC+AGG | + | chr7.3:23761365-23761384 | MS.gene52281:CDS | 35.0% | |
| AAGATGTTGGGAGATGAATT+TGG | + | chr7.3:23760926-23760945 | MS.gene52281:CDS | 35.0% | |
| ACTCATTGTATGGGAGAATT+TGG | + | chr7.3:23761741-23761760 | MS.gene52281:CDS | 35.0% | |
| ATTCAAGAGAAACGCTTGAT+AGG | + | chr7.3:23761625-23761644 | MS.gene52281:CDS | 35.0% | |
| ATTCTTGTCTGATTCACCAT+CGG | - | chr7.3:23760792-23760811 | None:intergenic | 35.0% | |
| CTAGAATCTGATGACAAGTT+TGG | + | chr7.3:23761196-23761215 | MS.gene52281:CDS | 35.0% | |
| CTTTATTACAATCTCACCAG+TGG | - | chr7.3:23761797-23761816 | None:intergenic | 35.0% | |
| GCAAGCAAAAATATTGAACG+TGG | + | chr7.3:23761522-23761541 | MS.gene52281:CDS | 35.0% | |
| GCGAATGAGTACAAAAGATT+TGG | + | chr7.3:23761919-23761938 | MS.gene52281:CDS | 35.0% | |
| GGGAGAATTTGGATATTACT+AGG | + | chr7.3:23761752-23761771 | MS.gene52281:CDS | 35.0% | |
| GGTTGAAACACTCATTGTAT+GGG | + | chr7.3:23761732-23761751 | MS.gene52281:CDS | 35.0% | |
| GTTGATCTACCAAAGAAACA+TGG | + | chr7.3:23761298-23761317 | MS.gene52281:CDS | 35.0% | |
| TAAAGAACCAATCCATTTGG+AGG | - | chr7.3:23761046-23761065 | None:intergenic | 35.0% | |
| TATAACAAGGTTCCTCCAAA+TGG | + | chr7.3:23761031-23761050 | MS.gene52281:CDS | 35.0% | |
| TGACCAACAAATCTCAAGAA+GGG | + | chr7.3:23761959-23761978 | MS.gene52281:CDS | 35.0% | |
| TGCTTCAAACATCAAGAGTA+GGG | + | chr7.3:23760952-23760971 | MS.gene52281:CDS | 35.0% | |
| ! | CAAAAAGTTGATCAAAGGAC+TGG | + | chr7.3:23760829-23760848 | MS.gene52281:CDS | 35.0% |
| ! | GATGAGTTATCTGATGATGA+TGG | + | chr7.3:23762051-23762070 | MS.gene52281:CDS | 35.0% |
| !! | ACATTGAAGGCTCTAGAAAT+GGG | + | chr7.3:23761706-23761725 | MS.gene52281:CDS | 35.0% |
| !! | GATGCATTGATTGGTTTGAA+AGG | - | chr7.3:23761127-23761146 | None:intergenic | 35.0% |
| !!! | AATTGTGTCGTTTTTCCATC+CGG | - | chr7.3:23761360-23761379 | None:intergenic | 35.0% |
| ACACGAGATATTTGATCACG+TGG | - | chr7.3:23760902-23760921 | None:intergenic | 40.0% | |
| ACATCAAGAGTAGGGTAAAC+CGG | + | chr7.3:23760960-23760979 | MS.gene52281:CDS | 40.0% | |
| AGAAGATGCCATTACTTGAG+TGG | + | chr7.3:23761893-23761912 | MS.gene52281:CDS | 40.0% | |
| AGTATGAGTCCAGCAACATT+AGG | - | chr7.3:23761442-23761461 | None:intergenic | 40.0% | |
| ATTAAGTGGAAATACTCGCG+AGG | + | chr7.3:23761258-23761277 | MS.gene52281:CDS | 40.0% | |
| CGAATGATCTGATATCAAGC+TGG | - | chr7.3:23762032-23762051 | None:intergenic | 40.0% | |
| CGGTTGAAACACTCATTGTA+TGG | + | chr7.3:23761731-23761750 | MS.gene52281:CDS | 40.0% | |
| CTACCAAAGAAACATGGTAG+AGG | + | chr7.3:23761304-23761323 | MS.gene52281:CDS | 40.0% | |
| CTGCTTCAAACATCAAGAGT+AGG | + | chr7.3:23760951-23760970 | MS.gene52281:CDS | 40.0% | |
| GTGACCAACAAATCTCAAGA+AGG | + | chr7.3:23761958-23761977 | MS.gene52281:CDS | 40.0% | |
| TAATGTTGCTGGACTCATAC+TGG | + | chr7.3:23761441-23761460 | MS.gene52281:CDS | 40.0% | |
| TCCACTTAATGTCCCGAAAA+GGG | - | chr7.3:23761248-23761267 | None:intergenic | 40.0% | |
| TTCCACTTAATGTCCCGAAA+AGG | - | chr7.3:23761249-23761268 | None:intergenic | 40.0% | |
| TTCGCAAACCACTCAAGTAA+TGG | - | chr7.3:23761904-23761923 | None:intergenic | 40.0% | |
| ! | ATCTCGTGTCACTAAGATGT+TGG | + | chr7.3:23760913-23760932 | MS.gene52281:CDS | 40.0% |
| ! | ATGGAAATGGAACCCTTTTC+GGG | + | chr7.3:23761233-23761252 | MS.gene52281:CDS | 40.0% |
| ! | GATGGAAATGGAACCCTTTT+CGG | + | chr7.3:23761232-23761251 | MS.gene52281:CDS | 40.0% |
| ! | TCTCGTGTCACTAAGATGTT+GGG | + | chr7.3:23760914-23760933 | MS.gene52281:CDS | 40.0% |
| !! | AGGGTTGATGTAAAACTGAG+TGG | - | chr7.3:23761407-23761426 | None:intergenic | 40.0% |
| !! | CACATTGAAGGCTCTAGAAA+TGG | + | chr7.3:23761705-23761724 | MS.gene52281:CDS | 40.0% |
| AACCTCCAAATCTGCAGATG+TGG | - | chr7.3:23761869-23761888 | None:intergenic | 45.0% | |
| ACATTAGGCTGACTAGTAGC+AGG | - | chr7.3:23761427-23761446 | None:intergenic | 45.0% | |
| ACTAGTCAGCCTAATGTTGC+TGG | + | chr7.3:23761430-23761449 | MS.gene52281:CDS | 45.0% | |
| ATCTGCAGATTTGGAGGTTC+AGG | + | chr7.3:23761870-23761889 | MS.gene52281:CDS | 45.0% | |
| CAAAAGATTTGGATGCTCGC+TGG | + | chr7.3:23761930-23761949 | MS.gene52281:CDS | 45.0% | |
| CAAGGTTCCTCCAAATGGAT+TGG | + | chr7.3:23761036-23761055 | MS.gene52281:CDS | 45.0% | |
| CATTAGGCTGACTAGTAGCA+GGG | - | chr7.3:23761426-23761445 | None:intergenic | 45.0% | |
| CCAAAGAAACATGGTAGAGG+AGG | + | chr7.3:23761307-23761326 | MS.gene52281:CDS | 45.0% | |
| CCATTACTTCTGCTCAACAG+AGG | + | chr7.3:23760999-23761018 | MS.gene52281:CDS | 45.0% | |
| CCTCCTCTACCATGTTTCTT+TGG | - | chr7.3:23761310-23761329 | None:intergenic | 45.0% | |
| CCTCTGTTGAGCAGAAGTAA+TGG | - | chr7.3:23761002-23761021 | None:intergenic | 45.0% | |
| CGACCCTTCTTGAGATTTGT+TGG | - | chr7.3:23761965-23761984 | None:intergenic | 45.0% | |
| CGAGATATTTGATCACGTGG+AGG | - | chr7.3:23760899-23760918 | None:intergenic | 45.0% | |
| GAAGAGATAAGCCAAGAGAC+TGG | + | chr7.3:23761658-23761677 | MS.gene52281:CDS | 45.0% | |
| GTGCTGAAAAATGCTACCAC+TGG | + | chr7.3:23761778-23761797 | MS.gene52281:CDS | 45.0% | |
| ! | AACGTGGTTGATGTCTCGTA+TGG | + | chr7.3:23761538-23761557 | MS.gene52281:CDS | 45.0% |
| ! | TGGTTGATGTCTCGTATGGT+GGG | + | chr7.3:23761542-23761561 | MS.gene52281:CDS | 45.0% |
| !! | ACCCTTTTCGGGACATTAAG+TGG | + | chr7.3:23761244-23761263 | MS.gene52281:CDS | 45.0% |
| !! | CGGAGTTGATGACACATTGA+AGG | + | chr7.3:23761693-23761712 | MS.gene52281:CDS | 45.0% |
| !! | TGATGATGATGGAGTGTACG+AGG | + | chr7.3:23762062-23762081 | MS.gene52281:CDS | 45.0% |
| AAAGGACTGGAAGCTGCAAG+AGG | + | chr7.3:23760842-23760861 | MS.gene52281:CDS | 50.0% | |
| CTGGAAGCTGCAAGAGGTAA+TGG | + | chr7.3:23760848-23760867 | MS.gene52281:CDS | 50.0% | |
| GGTACCATTCTCACTGAGGA+CGG | + | chr7.3:23761070-23761089 | MS.gene52281:CDS | 50.0% | |
| GTAAACCGGCAATCAGTGCT+TGG | + | chr7.3:23760974-23760993 | MS.gene52281:CDS | 50.0% | |
| GTCCTCAGTGAGAATGGTAC+CGG | - | chr7.3:23761071-23761090 | None:intergenic | 50.0% | |
| TACCGGTACCATTCTCACTG+AGG | + | chr7.3:23761066-23761085 | MS.gene52281:CDS | 50.0% | |
| TGAGGACGGCAAGGAAAAGA+AGG | + | chr7.3:23761084-23761103 | MS.gene52281:CDS | 50.0% | |
| ! | AAGGGTCGCAGTTTTGCAGA+GGG | + | chr7.3:23761977-23761996 | MS.gene52281:CDS | 50.0% |
| ! | CAGTTTTGCAGAGGGTTTGG+TGG | + | chr7.3:23761985-23762004 | MS.gene52281:CDS | 50.0% |
| ! | CTTTGCGTTTTGCTCGTCTC+CGG | + | chr7.3:23761338-23761357 | MS.gene52281:CDS | 50.0% |
| ! | GGTTGATGTCTCGTATGGTG+GGG | + | chr7.3:23761543-23761562 | MS.gene52281:CDS | 50.0% |
| ! | GTCTCGTATGGTGGGGAAAA+TGG | + | chr7.3:23761550-23761569 | MS.gene52281:CDS | 50.0% |
| ! | GTGGTTGATGTCTCGTATGG+TGG | + | chr7.3:23761541-23761560 | MS.gene52281:CDS | 50.0% |
| ! | TCGCAGTTTTGCAGAGGGTT+TGG | + | chr7.3:23761982-23762001 | MS.gene52281:CDS | 50.0% |
| !! | AGAGGGTTTGGTGGTATTGG+AGG | + | chr7.3:23761994-23762013 | MS.gene52281:CDS | 50.0% |
| !! | GAAGGCTCTAGAAATGGGTG+CGG | + | chr7.3:23761711-23761730 | MS.gene52281:CDS | 50.0% |
| !! | TGCAGAGGGTTTGGTGGTAT+TGG | + | chr7.3:23761991-23762010 | MS.gene52281:CDS | 50.0% |
| CATTCTCACTGAGGACGGCA+AGG | + | chr7.3:23761075-23761094 | MS.gene52281:CDS | 55.0% | |
| CTTGCCGTCCTCAGTGAGAA+TGG | - | chr7.3:23761077-23761096 | None:intergenic | 55.0% | |
| GGCCACATCTGCAGATTTGG+AGG | + | chr7.3:23761864-23761883 | MS.gene52281:CDS | 55.0% | |
| GTTGCTGGACTCATACTGGC+TGG | + | chr7.3:23761445-23761464 | MS.gene52281:CDS | 55.0% | |
| TCAAAGCCACTTCCGCGATG+CGG | + | chr7.3:23761843-23761862 | MS.gene52281:CDS | 55.0% | |
| TGCGGCCACATCTGCAGATT+TGG | + | chr7.3:23761861-23761880 | MS.gene52281:CDS | 55.0% | |
| ! | GAAGGGTCGCAGTTTTGCAG+AGG | + | chr7.3:23761976-23761995 | MS.gene52281:CDS | 55.0% |
| !! | TGGCTCCAAGCACTGATTGC+CGG | - | chr7.3:23760982-23761001 | None:intergenic | 55.0% |
| ! | GCGTTTTGCTCGTCTCCGGA+TGG | + | chr7.3:23761342-23761361 | MS.gene52281:CDS | 60.0% |
| ATGTGGCCGCATCGCGGAAG+TGG | - | chr7.3:23761852-23761871 | None:intergenic | 65.0% | |
| CTGCAGATGTGGCCGCATCG+CGG | - | chr7.3:23761858-23761877 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 23760785 | 23762095 | 23760785 | ID=MS.gene52281 |
| chr7.3 | mRNA | 23760785 | 23762095 | 23760785 | ID=MS.gene52281.t1;Parent=MS.gene52281 |
| chr7.3 | exon | 23760785 | 23762095 | 23760785 | ID=MS.gene52281.t1.exon1;Parent=MS.gene52281.t1 |
| chr7.3 | CDS | 23760785 | 23762095 | 23760785 | ID=cds.MS.gene52281.t1;Parent=MS.gene52281.t1 |
| Gene Sequence |
| Protein sequence |
