Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44809.t1 | XP_024640086.1 | 97 | 763 | 23 | 0 | 35 | 797 | 37 | 799 | 0.00E+00 | 1389.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44809.t1 | P73625 | 31.1 | 782 | 430 | 16 | 41 | 796 | 8 | 706 | 3.5e-77 | 291.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44809.t1 | G7K652 | 96.9 | 763 | 23 | 1 | 35 | 797 | 37 | 798 | 0.0e+00 | 1383.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene44809 | 0.844443 | 7.54E-59 | -1.69E-46 |
| MS.gene049217 | MS.gene44809 | 0.803988 | 2.68E-49 | -1.69E-46 |
| MS.gene049222 | MS.gene44809 | 0.827285 | 1.70E-54 | -1.69E-46 |
| MS.gene049356 | MS.gene44809 | 0.827161 | 1.82E-54 | -1.69E-46 |
| MS.gene049488 | MS.gene44809 | 0.80856 | 2.92E-50 | -1.69E-46 |
| MS.gene049609 | MS.gene44809 | 0.847211 | 1.34E-59 | -1.69E-46 |
| MS.gene049805 | MS.gene44809 | 0.815639 | 8.34E-52 | -1.69E-46 |
| MS.gene050122 | MS.gene44809 | 0.82252 | 2.27E-53 | -1.69E-46 |
| MS.gene050520 | MS.gene44809 | 0.806278 | 8.89E-50 | -1.69E-46 |
| MS.gene050593 | MS.gene44809 | 0.812111 | 5.00E-51 | -1.69E-46 |
| MS.gene050670 | MS.gene44809 | 0.841536 | 4.48E-58 | -1.69E-46 |
| MS.gene050747 | MS.gene44809 | 0.860044 | 2.72E-63 | -1.69E-46 |
| MS.gene050772 | MS.gene44809 | 0.820473 | 6.73E-53 | -1.69E-46 |
| MS.gene05080 | MS.gene44809 | 0.819194 | 1.32E-52 | -1.69E-46 |
| MS.gene050950 | MS.gene44809 | 0.840218 | 9.92E-58 | -1.69E-46 |
| MS.gene05105 | MS.gene44809 | 0.857075 | 2.09E-62 | -1.69E-46 |
| MS.gene051360 | MS.gene44809 | 0.803743 | 3.01E-49 | -1.69E-46 |
| MS.gene051387 | MS.gene44809 | 0.860731 | 1.68E-63 | -1.69E-46 |
| MS.gene051525 | MS.gene44809 | 0.822073 | 2.88E-53 | -1.69E-46 |
| MS.gene051666 | MS.gene44809 | 0.803778 | 2.96E-49 | -1.69E-46 |
| MS.gene051838 | MS.gene44809 | 0.83167 | 1.46E-55 | -1.69E-46 |
| MS.gene051941 | MS.gene44809 | 0.818591 | 1.81E-52 | -1.69E-46 |
| MS.gene052164 | MS.gene44809 | 0.82875 | 7.56E-55 | -1.69E-46 |
| MS.gene052346 | MS.gene44809 | 0.83986 | 1.23E-57 | -1.69E-46 |
| MS.gene052414 | MS.gene44809 | 0.848265 | 6.84E-60 | -1.69E-46 |
| MS.gene052421 | MS.gene44809 | 0.847518 | 1.10E-59 | -1.69E-46 |
| MS.gene052485 | MS.gene44809 | 0.804787 | 1.83E-49 | -1.69E-46 |
| MS.gene052501 | MS.gene44809 | 0.839139 | 1.89E-57 | -1.69E-46 |
| MS.gene052503 | MS.gene44809 | 0.824092 | 9.73E-54 | -1.69E-46 |
| MS.gene052505 | MS.gene44809 | 0.844291 | 8.28E-59 | -1.69E-46 |
| MS.gene052615 | MS.gene44809 | 0.824794 | 6.66E-54 | -1.69E-46 |
| MS.gene052823 | MS.gene44809 | 0.820763 | 5.77E-53 | -1.69E-46 |
| MS.gene052825 | MS.gene44809 | 0.817852 | 2.66E-52 | -1.69E-46 |
| MS.gene052982 | MS.gene44809 | 0.850324 | 1.83E-60 | -1.69E-46 |
| MS.gene052999 | MS.gene44809 | 0.801714 | 7.90E-49 | -1.69E-46 |
| MS.gene053203 | MS.gene44809 | 0.803316 | 3.70E-49 | -1.69E-46 |
| MS.gene053207 | MS.gene44809 | 0.833237 | 5.99E-56 | -1.69E-46 |
| MS.gene053213 | MS.gene44809 | 0.806864 | 6.69E-50 | -1.69E-46 |
| MS.gene053436 | MS.gene44809 | 0.838183 | 3.35E-57 | -1.69E-46 |
| MS.gene053480 | MS.gene44809 | 0.80765 | 4.56E-50 | -1.69E-46 |
| MS.gene053574 | MS.gene44809 | 0.839102 | 1.94E-57 | -1.69E-46 |
| MS.gene053626 | MS.gene44809 | 0.853686 | 2.03E-61 | -1.69E-46 |
| MS.gene053632 | MS.gene44809 | 0.829458 | 5.09E-55 | -1.69E-46 |
| MS.gene053824 | MS.gene44809 | 0.809983 | 1.44E-50 | -1.69E-46 |
| MS.gene053873 | MS.gene44809 | 0.823656 | 1.23E-53 | -1.69E-46 |
| MS.gene053912 | MS.gene44809 | 0.828167 | 1.04E-54 | -1.69E-46 |
| MS.gene054595 | MS.gene44809 | 0.803678 | 3.11E-49 | -1.69E-46 |
| MS.gene054600 | MS.gene44809 | 0.808633 | 2.81E-50 | -1.69E-46 |
| MS.gene054719 | MS.gene44809 | 0.817928 | 2.56E-52 | -1.69E-46 |
| MS.gene054917 | MS.gene44809 | 0.801186 | 1.01E-48 | -1.69E-46 |
| MS.gene054920 | MS.gene44809 | 0.80222 | 6.22E-49 | -1.69E-46 |
| MS.gene054927 | MS.gene44809 | 0.885293 | 9.21E-72 | -1.69E-46 |
| MS.gene05494 | MS.gene44809 | 0.807709 | 4.43E-50 | -1.69E-46 |
| MS.gene05505 | MS.gene44809 | 0.841905 | 3.58E-58 | -1.69E-46 |
| MS.gene055369 | MS.gene44809 | 0.805994 | 1.02E-49 | -1.69E-46 |
| MS.gene055486 | MS.gene44809 | 0.815189 | 1.05E-51 | -1.69E-46 |
| MS.gene055539 | MS.gene44809 | 0.825096 | 5.65E-54 | -1.69E-46 |
| MS.gene055577 | MS.gene44809 | 0.801658 | 8.12E-49 | -1.69E-46 |
| MS.gene055722 | MS.gene44809 | 0.802995 | 4.31E-49 | -1.69E-46 |
| MS.gene055741 | MS.gene44809 | 0.850087 | 2.13E-60 | -1.69E-46 |
| MS.gene055744 | MS.gene44809 | 0.843663 | 1.22E-58 | -1.69E-46 |
| MS.gene055909 | MS.gene44809 | 0.851103 | 1.10E-60 | -1.69E-46 |
| MS.gene055911 | MS.gene44809 | 0.857511 | 1.55E-62 | -1.69E-46 |
| MS.gene055941 | MS.gene44809 | 0.81692 | 4.31E-52 | -1.69E-46 |
| MS.gene05594 | MS.gene44809 | 0.803932 | 2.75E-49 | -1.69E-46 |
| MS.gene05601 | MS.gene44809 | 0.815338 | 9.73E-52 | -1.69E-46 |
| MS.gene05602 | MS.gene44809 | 0.818279 | 2.13E-52 | -1.69E-46 |
| MS.gene056040 | MS.gene44809 | 0.840942 | 6.41E-58 | -1.69E-46 |
| MS.gene056166 | MS.gene44809 | 0.842752 | 2.13E-58 | -1.69E-46 |
| MS.gene056407 | MS.gene44809 | 0.801174 | 1.02E-48 | -1.69E-46 |
| MS.gene056449 | MS.gene44809 | 0.814443 | 1.54E-51 | -1.69E-46 |
| MS.gene056511 | MS.gene44809 | 0.824905 | 6.27E-54 | -1.69E-46 |
| MS.gene056514 | MS.gene44809 | 0.815345 | 9.69E-52 | -1.69E-46 |
| MS.gene05664 | MS.gene44809 | 0.815557 | 8.70E-52 | -1.69E-46 |
| MS.gene05666 | MS.gene44809 | 0.82307 | 1.69E-53 | -1.69E-46 |
| MS.gene056782 | MS.gene44809 | 0.802407 | 5.69E-49 | -1.69E-46 |
| MS.gene057019 | MS.gene44809 | 0.812858 | 3.43E-51 | -1.69E-46 |
| MS.gene057048 | MS.gene44809 | 0.832545 | 8.89E-56 | -1.69E-46 |
| MS.gene057076 | MS.gene44809 | 0.829994 | 3.77E-55 | -1.69E-46 |
| MS.gene057124 | MS.gene44809 | 0.804246 | 2.37E-49 | -1.69E-46 |
| MS.gene057146 | MS.gene44809 | 0.817331 | 3.49E-52 | -1.69E-46 |
| MS.gene057289 | MS.gene44809 | 0.846756 | 1.78E-59 | -1.69E-46 |
| MS.gene057416 | MS.gene44809 | 0.807326 | 5.34E-50 | -1.69E-46 |
| MS.gene057734 | MS.gene44809 | 0.842379 | 2.68E-58 | -1.69E-46 |
| MS.gene057749 | MS.gene44809 | 0.840186 | 1.01E-57 | -1.69E-46 |
| MS.gene057823 | MS.gene44809 | 0.862954 | 3.51E-64 | -1.69E-46 |
| MS.gene057825 | MS.gene44809 | 0.808891 | 2.48E-50 | -1.69E-46 |
| MS.gene057831 | MS.gene44809 | 0.816642 | 4.98E-52 | -1.69E-46 |
| MS.gene05787 | MS.gene44809 | 0.807684 | 4.48E-50 | -1.69E-46 |
| MS.gene058007 | MS.gene44809 | 0.815335 | 9.75E-52 | -1.69E-46 |
| MS.gene058019 | MS.gene44809 | 0.825092 | 5.66E-54 | -1.69E-46 |
| MS.gene058090 | MS.gene44809 | 0.833979 | 3.91E-56 | -1.69E-46 |
| MS.gene058120 | MS.gene44809 | 0.871384 | 7.12E-67 | -1.69E-46 |
| MS.gene058130 | MS.gene44809 | 0.814201 | 1.74E-51 | -1.69E-46 |
| MS.gene058338 | MS.gene44809 | 0.809051 | 2.29E-50 | -1.69E-46 |
| MS.gene058376 | MS.gene44809 | 0.830995 | 2.14E-55 | -1.69E-46 |
| MS.gene05837 | MS.gene44809 | 0.814816 | 1.27E-51 | -1.69E-46 |
| MS.gene058444 | MS.gene44809 | 0.816157 | 6.39E-52 | -1.69E-46 |
| MS.gene058700 | MS.gene44809 | 0.850586 | 1.54E-60 | -1.69E-46 |
| MS.gene058838 | MS.gene44809 | 0.805129 | 1.55E-49 | -1.69E-46 |
| MS.gene058948 | MS.gene44809 | 0.869888 | 2.21E-66 | -1.69E-46 |
| MS.gene059023 | MS.gene44809 | 0.809786 | 1.59E-50 | -1.69E-46 |
| MS.gene05934 | MS.gene44809 | 0.813737 | 2.20E-51 | -1.69E-46 |
| MS.gene059367 | MS.gene44809 | 0.822464 | 2.34E-53 | -1.69E-46 |
| MS.gene059368 | MS.gene44809 | 0.835987 | 1.22E-56 | -1.69E-46 |
| MS.gene059420 | MS.gene44809 | 0.833204 | 6.10E-56 | -1.69E-46 |
| MS.gene059455 | MS.gene44809 | 0.827224 | 1.76E-54 | -1.69E-46 |
| MS.gene059667 | MS.gene44809 | 0.817883 | 2.62E-52 | -1.69E-46 |
| MS.gene059688 | MS.gene44809 | 0.802772 | 4.79E-49 | -1.69E-46 |
| MS.gene059711 | MS.gene44809 | 0.808949 | 2.41E-50 | -1.69E-46 |
| MS.gene059718 | MS.gene44809 | 0.820603 | 6.29E-53 | -1.69E-46 |
| MS.gene060372 | MS.gene44809 | 0.828383 | 9.27E-55 | -1.69E-46 |
| MS.gene060485 | MS.gene44809 | 0.811599 | 6.46E-51 | -1.69E-46 |
| MS.gene060835 | MS.gene44809 | 0.843987 | 9.99E-59 | -1.69E-46 |
| MS.gene060837 | MS.gene44809 | 0.844897 | 5.69E-59 | -1.69E-46 |
| MS.gene06092 | MS.gene44809 | 0.812351 | 4.43E-51 | -1.69E-46 |
| MS.gene061154 | MS.gene44809 | 0.816008 | 6.90E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44809.t1 | MTR_5g068600 | 96.496 | 799 | 25 | 2 | 1 | 797 | 1 | 798 | 0.0 | 1560 |
| MS.gene44809.t1 | MTR_5g068600 | 96.853 | 699 | 21 | 1 | 99 | 797 | 1 | 698 | 0.0 | 1374 |
| MS.gene44809.t1 | MTR_4g088920 | 30.676 | 828 | 491 | 26 | 9 | 796 | 5 | 789 | 3.74e-85 | 290 |
| MS.gene44809.t1 | MTR_1g045670 | 32.821 | 195 | 121 | 4 | 429 | 618 | 764 | 953 | 4.49e-21 | 99.8 |
| MS.gene44809.t1 | MTR_7g109470 | 30.116 | 259 | 149 | 9 | 417 | 646 | 1013 | 1268 | 3.72e-18 | 90.1 |
| MS.gene44809.t1 | MTR_1g100683 | 27.753 | 227 | 131 | 5 | 431 | 628 | 799 | 1021 | 5.58e-16 | 83.2 |
| MS.gene44809.t1 | MTR_1g100683 | 27.753 | 227 | 131 | 5 | 431 | 628 | 799 | 1021 | 5.58e-16 | 83.2 |
| MS.gene44809.t1 | MTR_4g111945 | 28.846 | 208 | 133 | 3 | 417 | 610 | 649 | 855 | 1.73e-15 | 81.3 |
| MS.gene44809.t1 | MTR_6g086270 | 27.397 | 219 | 142 | 5 | 408 | 610 | 870 | 1087 | 2.21e-15 | 81.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44809.t1 | AT5G54090 | 56.447 | 760 | 307 | 7 | 45 | 795 | 50 | 794 | 0.0 | 821 |
| MS.gene44809.t1 | AT1G65070 | 30.964 | 788 | 439 | 26 | 41 | 796 | 45 | 759 | 6.92e-71 | 249 |
| MS.gene44809.t1 | AT1G65070 | 30.964 | 788 | 439 | 26 | 41 | 796 | 45 | 759 | 8.72e-71 | 249 |
| MS.gene44809.t1 | AT1G65070 | 30.964 | 788 | 439 | 26 | 41 | 796 | 45 | 759 | 1.14e-70 | 249 |
| MS.gene44809.t1 | AT1G65070 | 33.788 | 586 | 319 | 16 | 41 | 610 | 45 | 577 | 4.89e-68 | 238 |
| MS.gene44809.t1 | AT3G24320 | 35.000 | 240 | 137 | 5 | 418 | 639 | 498 | 736 | 1.41e-23 | 107 |
| MS.gene44809.t1 | AT3G24320 | 35.000 | 240 | 137 | 5 | 418 | 639 | 743 | 981 | 1.99e-23 | 107 |
| MS.gene44809.t1 | AT4G25540 | 29.000 | 200 | 130 | 2 | 431 | 619 | 814 | 1012 | 1.37e-16 | 85.1 |
| MS.gene44809.t1 | AT3G18524 | 28.155 | 206 | 135 | 3 | 417 | 610 | 648 | 852 | 8.51e-16 | 82.4 |
| MS.gene44809.t1 | AT3G24495 | 29.032 | 217 | 125 | 6 | 416 | 608 | 827 | 1038 | 1.40e-15 | 82.0 |
| MS.gene44809.t1 | AT4G02070 | 28.085 | 235 | 148 | 6 | 424 | 642 | 1073 | 1302 | 8.09e-15 | 79.7 |
| MS.gene44809.t1 | AT4G02070 | 28.085 | 235 | 148 | 6 | 424 | 642 | 1070 | 1299 | 8.44e-15 | 79.3 |
| MS.gene44809.t1 | AT4G17380 | 28.409 | 264 | 162 | 8 | 422 | 670 | 541 | 792 | 1.20e-13 | 75.5 |
Find 159 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTTTAAGTTGTAACCTTTC+TGG | 0.081692 | 5.4:-53598610 | None:intergenic |
| TCGCTGCAACTCATCATTTA+AGG | 0.127126 | 5.4:-53598538 | None:intergenic |
| GTATTGACTACCTGTATTTC+AGG | 0.165492 | 5.4:-53606767 | None:intergenic |
| CTTCAATAACAGTTTGTTAA+TGG | 0.187026 | 5.4:-53595574 | None:intergenic |
| TGTTTAGGTACAACAATTAA+TGG | 0.219118 | 5.4:+53598183 | MS.gene44809:intron |
| ATGGACCATTACCTGAAAAT+AGG | 0.224654 | 5.4:+53599556 | MS.gene44809:CDS |
| TTTCTTCGTAAGTTTGATTC+TGG | 0.228833 | 5.4:-53596397 | None:intergenic |
| GGCTCTTGCAGGTTGCAATT+TGG | 0.231612 | 5.4:+53596470 | MS.gene44809:CDS |
| CTATGAGGCAAATGCTGTTT+TGG | 0.252961 | 5.4:+53596656 | MS.gene44809:CDS |
| TTCAATAACAGTTTGTTAAT+GGG | 0.269690 | 5.4:-53595573 | None:intergenic |
| TGATACACGAGAGCTCTTAA+TGG | 0.276229 | 5.4:-53609870 | None:intergenic |
| TCAGACTGCTGACACCTTGC+AGG | 0.278816 | 5.4:+53596686 | MS.gene44809:CDS |
| CCTATCAGGAAGAAATATAT+TGG | 0.282927 | 5.4:-53599487 | None:intergenic |
| TGAACTAATATTTCTCCTTT+TGG | 0.285242 | 5.4:-53610505 | None:intergenic |
| AGCCAACTTACAAGGTTCTA+TGG | 0.286415 | 5.4:+53607590 | MS.gene44809:CDS |
| TTCCTTTGCTACCACTTCCT+TGG | 0.307546 | 5.4:+53595704 | MS.gene44809:CDS |
| TTAAATTTGCCCTATGGCTC+TGG | 0.312716 | 5.4:-53599623 | None:intergenic |
| TGGACATATTGATGCTATAA+TGG | 0.314397 | 5.4:+53596490 | MS.gene44809:CDS |
| GCTACCTATGGTCTTTCATT+TGG | 0.321976 | 5.4:+53599453 | MS.gene44809:CDS |
| AATGTAGGTAATTACTGATA+TGG | 0.322333 | 5.4:+53608987 | MS.gene44809:intron |
| ATGTTCAGCGATCTTCCTTC+TGG | 0.335693 | 5.4:-53609720 | None:intergenic |
| CTATGGCTCTGGAGCAATAA+AGG | 0.344253 | 5.4:-53599612 | None:intergenic |
| TGCACAAACATGGCTCTTGC+AGG | 0.344470 | 5.4:+53596459 | MS.gene44809:CDS |
| GTTATAACTGGCCCCAATAC+TGG | 0.346782 | 5.4:+53600034 | MS.gene44809:CDS |
| CAAAGTGTCATTCCTTGTTA+AGG | 0.350745 | 5.4:-53599532 | None:intergenic |
| TGAAAAGATACTAGACAGTT+TGG | 0.352341 | 5.4:+53598928 | MS.gene44809:CDS |
| CTCATAGCCAGTTACCAGTA+TGG | 0.353942 | 5.4:-53596640 | None:intergenic |
| CTGAATCTGTGCAAATTCCT+TGG | 0.354114 | 5.4:+53606099 | MS.gene44809:CDS |
| TCCTTTGCTACCACTTCCTT+GGG | 0.356259 | 5.4:+53595705 | MS.gene44809:CDS |
| TTCCTAGTGCTGCTCCTTCA+AGG | 0.357480 | 5.4:-53606668 | None:intergenic |
| AATGGCTTTGCCGTGCTGTA+AGG | 0.358465 | 5.4:-53609852 | None:intergenic |
| TTAATTACCACTTCTGTAAG+AGG | 0.366319 | 5.4:-53596762 | None:intergenic |
| TGGACCATTACCTGAAAATA+GGG | 0.372346 | 5.4:+53599557 | MS.gene44809:CDS |
| CCAATATATTTCTTCCTGAT+AGG | 0.373935 | 5.4:+53599487 | MS.gene44809:CDS |
| ACCTATGGTCTTTCATTTGG+AGG | 0.384153 | 5.4:+53599456 | MS.gene44809:CDS |
| TTCTTCGTAAGTTTGATTCT+GGG | 0.384225 | 5.4:-53596396 | None:intergenic |
| CTTTATTATCTGCTGCAGTA+TGG | 0.389255 | 5.4:-53609907 | None:intergenic |
| AGCACTAGGAATGTCATTAT+TGG | 0.390539 | 5.4:+53606680 | MS.gene44809:CDS |
| GATGCTGCTCGGAAGCTATA+TGG | 0.392553 | 5.4:+53608822 | MS.gene44809:CDS |
| GTAGAAGAGCTATTCCTATC+AGG | 0.394308 | 5.4:-53599501 | None:intergenic |
| TGGTGCATAAGAACTGATTC+TGG | 0.396015 | 5.4:+53598351 | MS.gene44809:CDS |
| ATGGGATAAACTCTCCGATT+TGG | 0.396419 | 5.4:+53595677 | MS.gene44809:CDS |
| ACTCGTGTTATAGTTATAAC+TGG | 0.397684 | 5.4:+53600022 | MS.gene44809:CDS |
| CAATAAAGGATGATAAGCTT+TGG | 0.402841 | 5.4:-53599598 | None:intergenic |
| ATACTGTATCACCAACCTTC+GGG | 0.410598 | 5.4:-53610435 | None:intergenic |
| CGGAGAACCATACTGGTAAC+TGG | 0.410863 | 5.4:+53596633 | MS.gene44809:CDS |
| CAGTCGCTGTCTACGTTCTC+TGG | 0.411226 | 5.4:+53606167 | MS.gene44809:CDS |
| CGCTGCAACTCATCATTTAA+GGG | 0.411337 | 5.4:-53598537 | None:intergenic |
| TATTGACTACCTGTATTTCA+GGG | 0.417934 | 5.4:-53606766 | None:intergenic |
| AACTGGTACTGGCTGTGGTT+GGG | 0.420362 | 5.4:-53599978 | None:intergenic |
| TGGTTGCCAGGTTTCTGCAC+TGG | 0.421893 | 5.4:+53599949 | MS.gene44809:intron |
| AATAAAGGATGATAAGCTTT+GGG | 0.422522 | 5.4:-53599597 | None:intergenic |
| CCATTCTTCACAAGAAGGTC+AGG | 0.423556 | 5.4:+53609804 | MS.gene44809:CDS |
| CTATCAGGAAGAAATATATT+GGG | 0.429322 | 5.4:-53599486 | None:intergenic |
| CCTGACCTTCTTGTGAAGAA+TGG | 0.435258 | 5.4:-53609804 | None:intergenic |
| GTTGCAGCGAGCAAGAAGTT+TGG | 0.436216 | 5.4:+53598551 | MS.gene44809:CDS |
| GTGCATTTCAACTGCAGCAT+TGG | 0.437777 | 5.4:-53596441 | None:intergenic |
| TTAAGGGTCTATTGTTGTCC+AGG | 0.437835 | 5.4:+53598388 | MS.gene44809:CDS |
| ACAAGGAATGACACTTTGAA+TGG | 0.439511 | 5.4:+53599537 | MS.gene44809:CDS |
| AGTACAAGTGCTTGAGAGAA+AGG | 0.443774 | 5.4:+53597743 | MS.gene44809:CDS |
| CCTTCTTTAAATTTGCCCTA+TGG | 0.444660 | 5.4:-53599629 | None:intergenic |
| AACTGAAAACCCTGAAATAC+AGG | 0.447137 | 5.4:+53606757 | MS.gene44809:CDS |
| ACAGTATATGTCTCTTCCCT+TGG | 0.447715 | 5.4:+53610451 | MS.gene44809:CDS |
| TGCAATTGTTAGGTTGGAGC+AGG | 0.449743 | 5.4:+53606636 | MS.gene44809:intron |
| TAGACTATCGTGAAGTCCTC+TGG | 0.451075 | 5.4:-53609690 | None:intergenic |
| CTTTATTGCTCCAGAGCCAT+AGG | 0.451572 | 5.4:+53599613 | MS.gene44809:CDS |
| CAGGAAGTGAATAACATTGA+TGG | 0.452273 | 5.4:+53598324 | MS.gene44809:intron |
| AAAATTGACAAATCATCAAC+AGG | 0.452823 | 5.4:+53609968 | MS.gene44809:CDS |
| TCCTAGTGCTGCTCCTTCAA+GGG | 0.457781 | 5.4:-53606667 | None:intergenic |
| GCCAACTTACAAGGTTCTAT+GGG | 0.462665 | 5.4:+53607591 | MS.gene44809:CDS |
| TGTAGGATTGGCTGCTATGA+TGG | 0.462827 | 5.4:+53600081 | MS.gene44809:CDS |
| CAATTAAGCAAGACAAAGAT+TGG | 0.463606 | 5.4:+53596724 | MS.gene44809:CDS |
| TCTGAGGCTGTCGTGATGAA+TGG | 0.467093 | 5.4:-53595646 | None:intergenic |
| CCCCATAGAACCTTGTAAGT+TGG | 0.474515 | 5.4:-53607592 | None:intergenic |
| TATACTGTATCACCAACCTT+CGG | 0.476934 | 5.4:-53610436 | None:intergenic |
| CGTAGACAGCGACTGTGATA+AGG | 0.487083 | 5.4:-53606159 | None:intergenic |
| AAGTGCACGGAGAACCATAC+TGG | 0.487855 | 5.4:+53596626 | MS.gene44809:CDS |
| ATTAATGCCCGTGCTACCTA+TGG | 0.488156 | 5.4:+53599441 | MS.gene44809:CDS |
| CAGTATATGTCTCTTCCCTT+GGG | 0.492310 | 5.4:+53610452 | MS.gene44809:CDS |
| TTTGGTAGTAGTTCAGGAGT+TGG | 0.494704 | 5.4:+53598486 | MS.gene44809:intron |
| CATACTGGTAACTGGCTATG+AGG | 0.501449 | 5.4:+53596641 | MS.gene44809:CDS |
| CAACTGGTACTGGCTGTGGT+TGG | 0.502088 | 5.4:-53599979 | None:intergenic |
| TCAATAACAGTTTGTTAATG+GGG | 0.505161 | 5.4:-53595572 | None:intergenic |
| GAATTTGAAGCCAACTTACA+AGG | 0.505756 | 5.4:+53607582 | MS.gene44809:CDS |
| GGCCTTTGAAAATGCATGTA+TGG | 0.506536 | 5.4:+53607546 | MS.gene44809:CDS |
| GAACAAGTTATTGATGAAGA+TGG | 0.508102 | 5.4:+53597397 | MS.gene44809:CDS |
| CCATAGGGCAAATTTAAAGA+AGG | 0.511275 | 5.4:+53599629 | MS.gene44809:CDS |
| AAGGTTCTATGGGGCATACC+TGG | 0.513653 | 5.4:+53607601 | MS.gene44809:CDS |
| AAATTTAAAGAAGGTTAAAG+AGG | 0.513673 | 5.4:+53599638 | MS.gene44809:CDS |
| TGATGAAGATGGCTCGATCA+AGG | 0.514144 | 5.4:+53597408 | MS.gene44809:CDS |
| TCTTGACATTGCTGCAGCCT+CGG | 0.514279 | 5.4:-53609780 | None:intergenic |
| GCTGCTATGATGGCAAAATC+AGG | 0.516602 | 5.4:+53600091 | MS.gene44809:CDS |
| CATGTTATCCAATACCTTGA+GGG | 0.517636 | 5.4:-53595736 | None:intergenic |
| GGAGAAATATTAGTTCAAGC+TGG | 0.520726 | 5.4:+53610511 | MS.gene44809:CDS |
| ATTAATATAGCCGAGAGACT+TGG | 0.524704 | 5.4:+53608780 | MS.gene44809:CDS |
| TCATTTCATGAGCAGTAATG+AGG | 0.526211 | 5.4:+53607525 | MS.gene44809:intron |
| CTTGGGTCGTCATGCCCTCA+AGG | 0.527296 | 5.4:+53595722 | MS.gene44809:CDS |
| TTAAAAGTGGACTCACCAAA+AGG | 0.527505 | 5.4:+53610490 | MS.gene44809:CDS |
| CCTAGTGCTGCTCCTTCAAG+GGG | 0.539485 | 5.4:-53606666 | None:intergenic |
| TTTCATGCCTCTTACAGAAG+TGG | 0.546450 | 5.4:+53596755 | MS.gene44809:CDS |
| CGCTGAGGCAGATGTGCTGT+TGG | 0.548446 | 5.4:+53598581 | MS.gene44809:CDS |
| TATTATCTGCTGCAGTATGG+AGG | 0.549853 | 5.4:-53609904 | None:intergenic |
| ACGATATGTTTGAAGACTGT+AGG | 0.550635 | 5.4:+53600064 | MS.gene44809:CDS |
| AAGTCAACTGGTACTGGCTG+TGG | 0.551805 | 5.4:-53599983 | None:intergenic |
| CATTACCTGAAAATAGGGAA+TGG | 0.553007 | 5.4:+53599562 | MS.gene44809:CDS |
| AATTTGTGTGCAGATTCAGC+TGG | 0.555685 | 5.4:+53598892 | MS.gene44809:intron |
| ACAATCAACAAGGCAGTCAC+TGG | 0.558467 | 5.4:+53606524 | MS.gene44809:CDS |
| CAGACTGCTGACACCTTGCA+GGG | 0.560042 | 5.4:+53596687 | MS.gene44809:CDS |
| AGAGGGCAGTCCAAGTCTCT+CGG | 0.562691 | 5.4:-53608790 | None:intergenic |
| AAGATCCATTCTTCACAAGA+AGG | 0.565193 | 5.4:+53609799 | MS.gene44809:CDS |
| TGAAAGACCATAGGTAGCAC+GGG | 0.568455 | 5.4:-53599448 | None:intergenic |
| GGCATGACGACCCAAGGAAG+TGG | 0.569103 | 5.4:-53595715 | None:intergenic |
| AGCAAAGGAAGAGACCAAAT+CGG | 0.569675 | 5.4:-53595691 | None:intergenic |
| TTACACTGATCTGTTTCAAG+TGG | 0.572433 | 5.4:-53606190 | None:intergenic |
| GCTGTTGGCATTAACCAGAA+AGG | 0.577787 | 5.4:+53598596 | MS.gene44809:CDS |
| CCCCTTGAAGGAGCAGCACT+AGG | 0.578143 | 5.4:+53606666 | MS.gene44809:CDS |
| ACAGCCTCAGAGTTCTCGAA+TGG | 0.579069 | 5.4:+53595658 | MS.gene44809:CDS |
| AAGTGAATAACATTGATGGC+AGG | 0.580225 | 5.4:+53598328 | MS.gene44809:CDS |
| CAGTTTGGTTCAACTAGATG+TGG | 0.581185 | 5.4:+53598943 | MS.gene44809:CDS |
| TCATGGAACAGGATCAACTA+TGG | 0.581640 | 5.4:+53596372 | MS.gene44809:intron |
| ATGAAAGACCATAGGTAGCA+CGG | 0.581720 | 5.4:-53599449 | None:intergenic |
| TTGGAATCTCTTGCTCATGA+CGG | 0.581994 | 5.4:+53606699 | MS.gene44809:CDS |
| TTTATTGCTCCAGAGCCATA+GGG | 0.585774 | 5.4:+53599614 | MS.gene44809:CDS |
| CTGCTTCAGTTGATAGCCGA+TGG | 0.587554 | 5.4:-53609029 | None:intergenic |
| TTTAAGGGAACAGCAGAAAG+TGG | 0.591563 | 5.4:-53598522 | None:intergenic |
| ATGTTTGAAGACTGTAGGAT+TGG | 0.593640 | 5.4:+53600069 | MS.gene44809:CDS |
| CAGCCTCAGAGTTCTCGAAT+GGG | 0.595079 | 5.4:+53595659 | MS.gene44809:CDS |
| CTTGAGGGCATGACGACCCA+AGG | 0.600818 | 5.4:-53595721 | None:intergenic |
| AAAGCTTAATCAGAAGTGAA+AGG | 0.610747 | 5.4:+53598206 | MS.gene44809:CDS |
| AGTTAAAACAAGATTACCAT+CGG | 0.612320 | 5.4:+53609013 | MS.gene44809:CDS |
| CTGTTGTTATAGATGCTGCT+CGG | 0.616278 | 5.4:+53608811 | MS.gene44809:CDS |
| CTACTGCTGAATCCTTAACA+AGG | 0.616424 | 5.4:+53599520 | MS.gene44809:CDS |
| ACCCAAGGAAGTGGTAGCAA+AGG | 0.621580 | 5.4:-53595706 | None:intergenic |
| GCAGCATCTATAACAACAGA+GGG | 0.625555 | 5.4:-53608807 | None:intergenic |
| TTCTAGCGCGGAGATTGATG+AGG | 0.626660 | 5.4:+53608848 | MS.gene44809:CDS |
| AGGTCTGCAGTCCCGAAGGT+TGG | 0.628526 | 5.4:+53610424 | MS.gene44809:CDS |
| AGATAGGTCTGCAGTCCCGA+AGG | 0.629467 | 5.4:+53610420 | MS.gene44809:intron |
| ATAGTCTATTGAAAACCAGA+AGG | 0.631248 | 5.4:+53609705 | MS.gene44809:CDS |
| ACTATAGCAACGACTCATCA+CGG | 0.639274 | 5.4:+53606732 | MS.gene44809:CDS |
| AAACTGCAATTCAAAGTGCA+CGG | 0.639724 | 5.4:+53596613 | MS.gene44809:CDS |
| CATTCTTCACAAGAAGGTCA+GGG | 0.647392 | 5.4:+53609805 | MS.gene44809:CDS |
| TCCTCCAAATGAAAGACCAT+AGG | 0.648761 | 5.4:-53599457 | None:intergenic |
| GCTATATGGTTCTTCTAGCG+CGG | 0.648768 | 5.4:+53608836 | MS.gene44809:CDS |
| TGTAATTGTAGGCAATCCAG+AGG | 0.649295 | 5.4:+53609674 | MS.gene44809:intron |
| GCAGTTGAAATGCACAAACA+TGG | 0.652904 | 5.4:+53596449 | MS.gene44809:CDS |
| GTCACTGGTGCTACTAGATG+AGG | 0.654433 | 5.4:+53606539 | MS.gene44809:CDS |
| GAAACAAAGACAAACCGTGT+TGG | 0.657134 | 5.4:+53595614 | MS.gene44809:CDS |
| GCTAAGAAAACCTTACAGCA+CGG | 0.661377 | 5.4:+53609842 | MS.gene44809:CDS |
| AGCAGCATCTATAACAACAG+AGG | 0.669740 | 5.4:-53608808 | None:intergenic |
| TATCCCATTCGAGAACTCTG+AGG | 0.679440 | 5.4:-53595662 | None:intergenic |
| CTAGTGCTGCTCCTTCAAGG+GGG | 0.682665 | 5.4:-53606665 | None:intergenic |
| AAAACTGAGAGATGTATCCG+AGG | 0.682810 | 5.4:+53609763 | MS.gene44809:CDS |
| TGAATAACATTGATGGCAGG+TGG | 0.692737 | 5.4:+53598331 | MS.gene44809:CDS |
| AATGGAAGCTGCAACCAACA+CGG | 0.695569 | 5.4:-53595628 | None:intergenic |
| CCAACTTACAAGGTTCTATG+GGG | 0.697470 | 5.4:+53607592 | MS.gene44809:CDS |
| TGGCTCGATCAAGGACTCTG+CGG | 0.705075 | 5.4:+53597417 | MS.gene44809:CDS |
| ATAACTGGCCCCAATACTGG+CGG | 0.712370 | 5.4:+53600037 | MS.gene44809:CDS |
| ATATTAAATTACAATCAACA+AGG | 0.731546 | 5.4:+53606514 | MS.gene44809:CDS |
| AAGTTTGGTAGCAAACGCTG+AGG | 0.732255 | 5.4:+53598566 | MS.gene44809:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 53595525 | 53610576 | 53595525 | ID=MS.gene44809 |
| chr5.4 | mRNA | 53595525 | 53610576 | 53595525 | ID=MS.gene44809.t1;Parent=MS.gene44809 |
| chr5.4 | exon | 53595525 | 53595743 | 53595525 | ID=MS.gene44809.t1.exon1;Parent=MS.gene44809.t1 |
| chr5.4 | CDS | 53595525 | 53595743 | 53595525 | ID=cds.MS.gene44809.t1;Parent=MS.gene44809.t1 |
| chr5.4 | exon | 53596383 | 53596511 | 53596383 | ID=MS.gene44809.t1.exon2;Parent=MS.gene44809.t1 |
| chr5.4 | CDS | 53596383 | 53596511 | 53596383 | ID=cds.MS.gene44809.t1;Parent=MS.gene44809.t1 |
| chr5.4 | exon | 53596609 | 53596776 | 53596609 | ID=MS.gene44809.t1.exon3;Parent=MS.gene44809.t1 |
| chr5.4 | CDS | 53596609 | 53596776 | 53596609 | ID=cds.MS.gene44809.t1;Parent=MS.gene44809.t1 |
| chr5.4 | exon | 53597355 | 53597438 | 53597355 | ID=MS.gene44809.t1.exon4;Parent=MS.gene44809.t1 |
| chr5.4 | CDS | 53597355 | 53597438 | 53597355 | ID=cds.MS.gene44809.t1;Parent=MS.gene44809.t1 |
| chr5.4 | exon | 53597714 | 53597764 | 53597714 | ID=MS.gene44809.t1.exon5;Parent=MS.gene44809.t1 |
| chr5.4 | CDS | 53597714 | 53597764 | 53597714 | ID=cds.MS.gene44809.t1;Parent=MS.gene44809.t1 |
| chr5.4 | exon | 53598190 | 53598246 | 53598190 | ID=MS.gene44809.t1.exon6;Parent=MS.gene44809.t1 |
| chr5.4 | CDS | 53598190 | 53598246 | 53598190 | ID=cds.MS.gene44809.t1;Parent=MS.gene44809.t1 |
| chr5.4 | exon | 53598327 | 53598409 | 53598327 | ID=MS.gene44809.t1.exon7;Parent=MS.gene44809.t1 |
| chr5.4 | CDS | 53598327 | 53598409 | 53598327 | ID=cds.MS.gene44809.t1;Parent=MS.gene44809.t1 |
| chr5.4 | exon | 53598494 | 53598617 | 53598494 | ID=MS.gene44809.t1.exon8;Parent=MS.gene44809.t1 |
| chr5.4 | CDS | 53598494 | 53598617 | 53598494 | ID=cds.MS.gene44809.t1;Parent=MS.gene44809.t1 |
| chr5.4 | exon | 53598905 | 53598964 | 53598905 | ID=MS.gene44809.t1.exon9;Parent=MS.gene44809.t1 |
| chr5.4 | CDS | 53598905 | 53598964 | 53598905 | ID=cds.MS.gene44809.t1;Parent=MS.gene44809.t1 |
| chr5.4 | exon | 53599441 | 53599680 | 53599441 | ID=MS.gene44809.t1.exon10;Parent=MS.gene44809.t1 |
| chr5.4 | CDS | 53599441 | 53599680 | 53599441 | ID=cds.MS.gene44809.t1;Parent=MS.gene44809.t1 |
| chr5.4 | exon | 53599959 | 53600112 | 53599959 | ID=MS.gene44809.t1.exon11;Parent=MS.gene44809.t1 |
| chr5.4 | CDS | 53599959 | 53600112 | 53599959 | ID=cds.MS.gene44809.t1;Parent=MS.gene44809.t1 |
| chr5.4 | exon | 53606081 | 53606208 | 53606081 | ID=MS.gene44809.t1.exon12;Parent=MS.gene44809.t1 |
| chr5.4 | CDS | 53606081 | 53606208 | 53606081 | ID=cds.MS.gene44809.t1;Parent=MS.gene44809.t1 |
| chr5.4 | exon | 53606513 | 53606560 | 53606513 | ID=MS.gene44809.t1.exon13;Parent=MS.gene44809.t1 |
| chr5.4 | CDS | 53606513 | 53606560 | 53606513 | ID=cds.MS.gene44809.t1;Parent=MS.gene44809.t1 |
| chr5.4 | exon | 53606648 | 53606778 | 53606648 | ID=MS.gene44809.t1.exon14;Parent=MS.gene44809.t1 |
| chr5.4 | CDS | 53606648 | 53606778 | 53606648 | ID=cds.MS.gene44809.t1;Parent=MS.gene44809.t1 |
| chr5.4 | exon | 53607540 | 53607622 | 53607540 | ID=MS.gene44809.t1.exon15;Parent=MS.gene44809.t1 |
| chr5.4 | CDS | 53607540 | 53607622 | 53607540 | ID=cds.MS.gene44809.t1;Parent=MS.gene44809.t1 |
| chr5.4 | exon | 53608766 | 53608869 | 53608766 | ID=MS.gene44809.t1.exon16;Parent=MS.gene44809.t1 |
| chr5.4 | CDS | 53608766 | 53608869 | 53608766 | ID=cds.MS.gene44809.t1;Parent=MS.gene44809.t1 |
| chr5.4 | exon | 53608994 | 53609064 | 53608994 | ID=MS.gene44809.t1.exon17;Parent=MS.gene44809.t1 |
| chr5.4 | CDS | 53608994 | 53609064 | 53608994 | ID=cds.MS.gene44809.t1;Parent=MS.gene44809.t1 |
| chr5.4 | exon | 53609685 | 53609989 | 53609685 | ID=MS.gene44809.t1.exon18;Parent=MS.gene44809.t1 |
| chr5.4 | CDS | 53609685 | 53609989 | 53609685 | ID=cds.MS.gene44809.t1;Parent=MS.gene44809.t1 |
| chr5.4 | exon | 53610422 | 53610576 | 53610422 | ID=MS.gene44809.t1.exon19;Parent=MS.gene44809.t1 |
| chr5.4 | CDS | 53610422 | 53610576 | 53610422 | ID=cds.MS.gene44809.t1;Parent=MS.gene44809.t1 |
| Gene Sequence |
| Protein sequence |
