Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46649.t1 | XP_003599257.1 | 93.4 | 106 | 5 | 2 | 1 | 104 | 1 | 106 | 7.30E-43 | 183 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46649.t1 | P31336 | 54.2 | 107 | 44 | 3 | 1 | 104 | 1 | 105 | 5.3e-18 | 91.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46649.t1 | G7IWU5 | 93.4 | 106 | 5 | 2 | 1 | 104 | 1 | 106 | 5.3e-43 | 183.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene46649 | 0.844145 | 9.06E-59 | -1.69E-46 |
| MS.gene049173 | MS.gene46649 | 0.881097 | 3.19E-70 | -1.69E-46 |
| MS.gene049217 | MS.gene46649 | 0.811622 | 6.39E-51 | -1.69E-46 |
| MS.gene049222 | MS.gene46649 | 0.823422 | 1.40E-53 | -1.69E-46 |
| MS.gene049285 | MS.gene46649 | 0.845314 | 4.39E-59 | -1.69E-46 |
| MS.gene049320 | MS.gene46649 | 0.841868 | 3.66E-58 | -1.69E-46 |
| MS.gene049609 | MS.gene46649 | 0.843293 | 1.53E-58 | -1.69E-46 |
| MS.gene049633 | MS.gene46649 | 0.824493 | 7.83E-54 | -1.69E-46 |
| MS.gene050163 | MS.gene46649 | 0.816326 | 5.86E-52 | -1.69E-46 |
| MS.gene050165 | MS.gene46649 | 0.809382 | 1.95E-50 | -1.69E-46 |
| MS.gene050333 | MS.gene46649 | 0.827644 | 1.40E-54 | -1.69E-46 |
| MS.gene050444 | MS.gene46649 | 0.802494 | 5.46E-49 | -1.69E-46 |
| MS.gene050504 | MS.gene46649 | 0.821657 | 3.59E-53 | -1.69E-46 |
| MS.gene050612 | MS.gene46649 | 0.813925 | 2.00E-51 | -1.69E-46 |
| MS.gene050675 | MS.gene46649 | 0.815166 | 1.06E-51 | -1.69E-46 |
| MS.gene050699 | MS.gene46649 | 0.820269 | 7.50E-53 | -1.69E-46 |
| MS.gene050747 | MS.gene46649 | 0.802654 | 5.06E-49 | -1.69E-46 |
| MS.gene050770 | MS.gene46649 | 0.801322 | 9.51E-49 | -1.69E-46 |
| MS.gene050772 | MS.gene46649 | 0.872082 | 4.18E-67 | -1.69E-46 |
| MS.gene050950 | MS.gene46649 | 0.803583 | 3.25E-49 | -1.69E-46 |
| MS.gene050986 | MS.gene46649 | 0.811011 | 8.66E-51 | -1.69E-46 |
| MS.gene05100 | MS.gene46649 | 0.8172 | 3.73E-52 | -1.69E-46 |
| MS.gene051228 | MS.gene46649 | 0.828981 | 6.64E-55 | -1.69E-46 |
| MS.gene051551 | MS.gene46649 | 0.821302 | 4.34E-53 | -1.69E-46 |
| MS.gene051666 | MS.gene46649 | 0.813814 | 2.12E-51 | -1.69E-46 |
| MS.gene051692 | MS.gene46649 | 0.824124 | 9.56E-54 | -1.69E-46 |
| MS.gene051810 | MS.gene46649 | 0.853845 | 1.82E-61 | -1.69E-46 |
| MS.gene051858 | MS.gene46649 | 0.84786 | 8.85E-60 | -1.69E-46 |
| MS.gene051949 | MS.gene46649 | 0.83255 | 8.87E-56 | -1.69E-46 |
| MS.gene052164 | MS.gene46649 | 0.820438 | 6.86E-53 | -1.69E-46 |
| MS.gene052271 | MS.gene46649 | 0.834633 | 2.68E-56 | -1.69E-46 |
| MS.gene052870 | MS.gene46649 | 0.82071 | 5.94E-53 | -1.69E-46 |
| MS.gene052872 | MS.gene46649 | 0.824432 | 8.10E-54 | -1.69E-46 |
| MS.gene052916 | MS.gene46649 | 0.801896 | 7.25E-49 | -1.69E-46 |
| MS.gene052966 | MS.gene46649 | 0.803105 | 4.09E-49 | -1.69E-46 |
| MS.gene053009 | MS.gene46649 | 0.867312 | 1.50E-65 | -1.69E-46 |
| MS.gene053203 | MS.gene46649 | 0.80431 | 2.30E-49 | -1.69E-46 |
| MS.gene053207 | MS.gene46649 | 0.842475 | 2.53E-58 | -1.69E-46 |
| MS.gene053213 | MS.gene46649 | 0.842384 | 2.67E-58 | -1.69E-46 |
| MS.gene053286 | MS.gene46649 | 0.840636 | 7.72E-58 | -1.69E-46 |
| MS.gene053574 | MS.gene46649 | 0.836875 | 7.24E-57 | -1.69E-46 |
| MS.gene053632 | MS.gene46649 | 0.833877 | 4.14E-56 | -1.69E-46 |
| MS.gene053824 | MS.gene46649 | 0.825328 | 4.98E-54 | -1.69E-46 |
| MS.gene053912 | MS.gene46649 | 0.811918 | 5.51E-51 | -1.69E-46 |
| MS.gene053916 | MS.gene46649 | 0.813233 | 2.84E-51 | -1.69E-46 |
| MS.gene054600 | MS.gene46649 | 0.856388 | 3.33E-62 | -1.69E-46 |
| MS.gene054642 | MS.gene46649 | 0.84863 | 5.42E-60 | -1.69E-46 |
| MS.gene054954 | MS.gene46649 | 0.829261 | 5.68E-55 | -1.69E-46 |
| MS.gene05505 | MS.gene46649 | 0.805209 | 1.49E-49 | -1.69E-46 |
| MS.gene055206 | MS.gene46649 | 0.815207 | 1.04E-51 | -1.69E-46 |
| MS.gene055212 | MS.gene46649 | 0.848178 | 7.23E-60 | -1.69E-46 |
| MS.gene055254 | MS.gene46649 | 0.802395 | 5.73E-49 | -1.69E-46 |
| MS.gene055362 | MS.gene46649 | 0.863886 | 1.81E-64 | -1.69E-46 |
| MS.gene055502 | MS.gene46649 | 0.818053 | 2.40E-52 | -1.69E-46 |
| MS.gene055577 | MS.gene46649 | 0.811634 | 6.35E-51 | -1.69E-46 |
| MS.gene055647 | MS.gene46649 | 0.805575 | 1.25E-49 | -1.69E-46 |
| MS.gene055722 | MS.gene46649 | 0.902392 | 1.02E-78 | -1.69E-46 |
| MS.gene055776 | MS.gene46649 | 0.80935 | 1.98E-50 | -1.69E-46 |
| MS.gene05592 | MS.gene46649 | 0.805388 | 1.37E-49 | -1.69E-46 |
| MS.gene056083 | MS.gene46649 | 0.839957 | 1.16E-57 | -1.69E-46 |
| MS.gene056374 | MS.gene46649 | 0.842816 | 2.05E-58 | -1.69E-46 |
| MS.gene056504 | MS.gene46649 | 0.820088 | 8.25E-53 | -1.69E-46 |
| MS.gene056510 | MS.gene46649 | 0.829218 | 5.82E-55 | -1.69E-46 |
| MS.gene056611 | MS.gene46649 | 0.800412 | 1.46E-48 | -1.69E-46 |
| MS.gene056657 | MS.gene46649 | 0.848622 | 5.45E-60 | -1.69E-46 |
| MS.gene056897 | MS.gene46649 | 0.822893 | 1.86E-53 | -1.69E-46 |
| MS.gene057129 | MS.gene46649 | 0.822201 | 2.69E-53 | -1.69E-46 |
| MS.gene057331 | MS.gene46649 | 0.832975 | 6.95E-56 | -1.69E-46 |
| MS.gene057416 | MS.gene46649 | 0.827869 | 1.23E-54 | -1.69E-46 |
| MS.gene057805 | MS.gene46649 | 0.837207 | 5.96E-57 | -1.69E-46 |
| MS.gene058419 | MS.gene46649 | 0.846219 | 2.49E-59 | -1.69E-46 |
| MS.gene058544 | MS.gene46649 | 0.812832 | 3.48E-51 | -1.69E-46 |
| MS.gene058723 | MS.gene46649 | 0.896682 | 2.92E-76 | -1.69E-46 |
| MS.gene058773 | MS.gene46649 | 0.861352 | 1.09E-63 | -1.69E-46 |
| MS.gene058838 | MS.gene46649 | 0.80927 | 2.06E-50 | -1.69E-46 |
| MS.gene059022 | MS.gene46649 | 0.862436 | 5.08E-64 | -1.69E-46 |
| MS.gene059023 | MS.gene46649 | 0.82353 | 1.32E-53 | -1.69E-46 |
| MS.gene059029 | MS.gene46649 | 0.811462 | 6.92E-51 | -1.69E-46 |
| MS.gene05913 | MS.gene46649 | 0.842389 | 2.66E-58 | -1.69E-46 |
| MS.gene059536 | MS.gene46649 | 0.818623 | 1.78E-52 | -1.69E-46 |
| MS.gene059677 | MS.gene46649 | 0.848777 | 4.94E-60 | -1.69E-46 |
| MS.gene059718 | MS.gene46649 | 0.852187 | 5.44E-61 | -1.69E-46 |
| MS.gene059756 | MS.gene46649 | 0.815111 | 1.09E-51 | -1.69E-46 |
| MS.gene059865 | MS.gene46649 | 0.837965 | 3.81E-57 | -1.69E-46 |
| MS.gene059902 | MS.gene46649 | 0.841338 | 5.05E-58 | -1.69E-46 |
| MS.gene060341 | MS.gene46649 | 0.825156 | 5.47E-54 | -1.69E-46 |
| MS.gene060356 | MS.gene46649 | 0.803815 | 2.91E-49 | -1.69E-46 |
| MS.gene060482 | MS.gene46649 | 0.822878 | 1.87E-53 | -1.69E-46 |
| MS.gene060703 | MS.gene46649 | 0.810955 | 8.91E-51 | -1.69E-46 |
| MS.gene060727 | MS.gene46649 | 0.806303 | 8.78E-50 | -1.69E-46 |
| MS.gene060762 | MS.gene46649 | 0.840181 | 1.01E-57 | -1.69E-46 |
| MS.gene06077 | MS.gene46649 | 0.800286 | 1.55E-48 | -1.69E-46 |
| MS.gene060804 | MS.gene46649 | 0.802208 | 6.26E-49 | -1.69E-46 |
| MS.gene060818 | MS.gene46649 | 0.804537 | 2.06E-49 | -1.69E-46 |
| MS.gene060837 | MS.gene46649 | 0.8141 | 1.83E-51 | -1.69E-46 |
| MS.gene061041 | MS.gene46649 | 0.809797 | 1.58E-50 | -1.69E-46 |
| MS.gene061150 | MS.gene46649 | 0.835285 | 1.83E-56 | -1.69E-46 |
| MS.gene061200 | MS.gene46649 | 0.818767 | 1.65E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46649.t1 | MTR_3g030850 | 93.396 | 106 | 5 | 2 | 1 | 104 | 1 | 106 | 3.04e-62 | 184 |
| MS.gene46649.t1 | MTR_3g030860 | 85.577 | 104 | 14 | 1 | 1 | 103 | 1 | 104 | 5.02e-51 | 156 |
| MS.gene46649.t1 | MTR_4g037505 | 71.963 | 107 | 26 | 4 | 1 | 103 | 1 | 107 | 7.14e-42 | 133 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46649.t1 | AT1G51400 | 57.407 | 108 | 40 | 3 | 1 | 104 | 1 | 106 | 1.43e-30 | 104 |
| MS.gene46649.t1 | AT3G21055 | 57.547 | 106 | 38 | 4 | 1 | 103 | 1 | 102 | 7.68e-25 | 90.5 |
| MS.gene46649.t1 | AT3G21055 | 57.547 | 106 | 38 | 4 | 1 | 103 | 41 | 142 | 1.63e-24 | 90.5 |
Find 23 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCCTCAATCCTCGGCTTCC+CGG | 0.362856 | 3.3:-23505377 | MS.gene46649:CDS |
| GTTGGCATAGTGACACAAAC+AGG | 0.491066 | 3.3:+23505127 | None:intergenic |
| AATGCTGTCCGAGCAGTTGA+AGG | 0.499596 | 3.3:-23505307 | MS.gene46649:CDS |
| GACAACAAGGAGGGAAGTAA+TGG | 0.504238 | 3.3:-23505265 | MS.gene46649:CDS |
| GCTTCCCGGCTGTAACCAAC+CGG | 0.507766 | 3.3:-23505363 | MS.gene46649:CDS |
| AGCCGGGAAGCCGAGGATTG+AGG | 0.531969 | 3.3:+23505375 | None:intergenic |
| TCTGCGATGCTACGGATGAC+CGG | 0.544879 | 3.3:+23505344 | None:intergenic |
| GAGTTTCCTCTGCGATGCTA+CGG | 0.551751 | 3.3:+23505336 | None:intergenic |
| GATGACCGGTTGGTTACAGC+CGG | 0.561463 | 3.3:+23505358 | None:intergenic |
| ATGCTGTCCGAGCAGTTGAA+GGG | 0.565277 | 3.3:-23505306 | MS.gene46649:CDS |
| GCCGAGGATTGAGGATGTCA+TGG | 0.565591 | 3.3:+23505384 | None:intergenic |
| TGGTTACAGCCGGGAAGCCG+AGG | 0.566698 | 3.3:+23505368 | None:intergenic |
| ACCATGACATCCTCAATCCT+CGG | 0.573002 | 3.3:-23505385 | MS.gene46649:CDS |
| GTGTCACTATGCCAACAGCT+AGG | 0.576409 | 3.3:-23505120 | MS.gene46649:CDS |
| TTGCGACAAATCCTAGCTGT+TGG | 0.581063 | 3.3:+23505109 | None:intergenic |
| TGCTGTCCGAGCAGTTGAAG+GGG | 0.590682 | 3.3:-23505305 | MS.gene46649:CDS |
| CGATGCTACGGATGACCGGT+TGG | 0.597909 | 3.3:+23505348 | None:intergenic |
| ATCAAATATGACAACAAGGA+GGG | 0.605176 | 3.3:-23505274 | MS.gene46649:CDS |
| ATGACCGGTTGGTTACAGCC+GGG | 0.647755 | 3.3:+23505359 | None:intergenic |
| ACAAGGAGGGAAGTAATGGA+AGG | 0.655922 | 3.3:-23505261 | MS.gene46649:CDS |
| GATCAAATATGACAACAAGG+AGG | 0.661670 | 3.3:-23505275 | MS.gene46649:CDS |
| GATGATCAAATATGACAACA+AGG | 0.678242 | 3.3:-23505278 | MS.gene46649:CDS |
| GGTCATCCGTAGCATCGCAG+AGG | 0.728401 | 3.3:-23505342 | MS.gene46649:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATCAAATATGACAACAAGGA+GGG | - | chr3.3:23505228-23505247 | MS.gene46649:CDS | 30.0% | |
| GATGATCAAATATGACAACA+AGG | - | chr3.3:23505224-23505243 | MS.gene46649:CDS | 30.0% | |
| !!! | ATACTTTTTCTTTGCTTCTG+GGG | + | chr3.3:23505352-23505371 | None:intergenic | 30.0% |
| !!! | CATACTTTTTCTTTGCTTCT+GGG | + | chr3.3:23505353-23505372 | None:intergenic | 30.0% |
| GATCAAATATGACAACAAGG+AGG | - | chr3.3:23505227-23505246 | MS.gene46649:CDS | 35.0% | |
| !!! | GCATACTTTTTCTTTGCTTC+TGG | + | chr3.3:23505354-23505373 | None:intergenic | 35.0% |
| GCAGATGATGAACCAAAAAG+AGG | - | chr3.3:23505324-23505343 | MS.gene46649:CDS | 40.0% | |
| ACCATGACATCCTCAATCCT+CGG | - | chr3.3:23505117-23505136 | MS.gene46649:CDS | 45.0% | |
| GACAACAAGGAGGGAAGTAA+TGG | - | chr3.3:23505237-23505256 | MS.gene46649:CDS | 45.0% | |
| GTTGGCATAGTGACACAAAC+AGG | + | chr3.3:23505378-23505397 | None:intergenic | 45.0% | |
| TTGCGACAAATCCTAGCTGT+TGG | + | chr3.3:23505396-23505415 | None:intergenic | 45.0% | |
| ! | ACAAGGAGGGAAGTAATGGA+AGG | - | chr3.3:23505241-23505260 | MS.gene46649:CDS | 45.0% |
| ! | TCTTTTCCCCTTCAACTGCT+CGG | + | chr3.3:23505206-23505225 | None:intergenic | 45.0% |
| !!! | CACTTTTGCTGGTGTTGCAA+TGG | - | chr3.3:23505302-23505321 | MS.gene46649:CDS | 45.0% |
| AATGCTGTCCGAGCAGTTGA+AGG | - | chr3.3:23505195-23505214 | MS.gene46649:CDS | 50.0% | |
| ATGCTGTCCGAGCAGTTGAA+GGG | - | chr3.3:23505196-23505215 | MS.gene46649:CDS | 50.0% | |
| GAGTTTCCTCTGCGATGCTA+CGG | + | chr3.3:23505169-23505188 | None:intergenic | 50.0% | |
| ! | GTGTCACTATGCCAACAGCT+AGG | - | chr3.3:23505382-23505401 | MS.gene46649:CDS | 50.0% |
| !! | GCTGCTGTTTGCACTTTTGC+TGG | - | chr3.3:23505291-23505310 | MS.gene46649:CDS | 50.0% |
| !!! | GCTTCTGGGGTACCTCTTTT+TGG | + | chr3.3:23505339-23505358 | None:intergenic | 50.0% |
| ATCCTCAATCCTCGGCTTCC+CGG | - | chr3.3:23505125-23505144 | MS.gene46649:CDS | 55.0% | |
| GCCGAGGATTGAGGATGTCA+TGG | + | chr3.3:23505121-23505140 | None:intergenic | 55.0% | |
| TCTGCGATGCTACGGATGAC+CGG | + | chr3.3:23505161-23505180 | None:intergenic | 55.0% | |
| TGCTGTCCGAGCAGTTGAAG+GGG | - | chr3.3:23505197-23505216 | MS.gene46649:CDS | 55.0% | |
| ! | ATGACCGGTTGGTTACAGCC+GGG | + | chr3.3:23505146-23505165 | None:intergenic | 55.0% |
| ! | GATGACCGGTTGGTTACAGC+CGG | + | chr3.3:23505147-23505166 | None:intergenic | 55.0% |
| CGATGCTACGGATGACCGGT+TGG | + | chr3.3:23505157-23505176 | None:intergenic | 60.0% | |
| GCTTCCCGGCTGTAACCAAC+CGG | - | chr3.3:23505139-23505158 | MS.gene46649:CDS | 60.0% | |
| GGTCATCCGTAGCATCGCAG+AGG | - | chr3.3:23505160-23505179 | MS.gene46649:CDS | 60.0% | |
| AGCCGGGAAGCCGAGGATTG+AGG | + | chr3.3:23505130-23505149 | None:intergenic | 65.0% | |
| TGGTTACAGCCGGGAAGCCG+AGG | + | chr3.3:23505137-23505156 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 23505105 | 23505419 | 23505105 | ID=MS.gene46649 |
| chr3.3 | mRNA | 23505105 | 23505419 | 23505105 | ID=MS.gene46649.t1;Parent=MS.gene46649 |
| chr3.3 | exon | 23505105 | 23505419 | 23505105 | ID=MS.gene46649.t1.exon1;Parent=MS.gene46649.t1 |
| chr3.3 | CDS | 23505105 | 23505419 | 23505105 | ID=cds.MS.gene46649.t1;Parent=MS.gene46649.t1 |
| Gene Sequence |
| Protein sequence |
