AlfalfaGEDB | MS.gene50662

M. sativa cultivar XinJiangDaYe / MS.gene50662

KEGG: K14488
GO: GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
PFAM: PF02519.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50662.t1	PNY03825.1	80.4	92	18	0	1	92	1	92	2.70E-33	151

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50662.t1	P33083	68.9	90	28	0	1	90	1	90	2.3e-28	125.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50662.t1	A0A2K3NLB0	80.4	92	18	0	1	92	1	92	2.0e-33	151.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene50662	MS.gene50675	PPI
MS.gene50662	MS.gene014670	PPI
MS.gene79890	MS.gene50662	PPI
MS.gene003615	MS.gene50662	PPI
MS.gene062973	MS.gene50662	PPI
MS.gene003602	MS.gene50662	PPI
MS.gene047517	MS.gene50662	PPI
MS.gene50662	MS.gene75314	PPI
MS.gene50662	MS.gene01764	PPI
MS.gene50662	MS.gene75679	PPI
MS.gene062984	MS.gene50662	PPI
MS.gene50662	MS.gene40325	PPI
MS.gene40327	MS.gene50662	PPI
MS.gene50662	MS.gene68760	PPI
MS.gene50662	MS.gene031198	PPI
MS.gene50662	MS.gene057797	PPI
MS.gene50662	MS.gene003614	PPI
MS.gene50662	MS.gene77528	PPI
MS.gene50662	MS.gene79886	PPI
MS.gene50662	MS.gene014671	PPI
MS.gene50662	MS.gene003596	PPI
MS.gene003587	MS.gene50662	PPI
MS.gene50662	MS.gene062988	PPI
MS.gene50662	MS.gene23371	PPI
MS.gene50662	MS.gene75672	PPI
MS.gene50662	MS.gene75674	PPI
MS.gene003590	MS.gene50662	PPI
MS.gene062943	MS.gene50662	PPI
MS.gene063112	MS.gene50662	PPI
MS.gene50662	MS.gene50654	PPI
MS.gene50662	MS.gene062974	PPI
MS.gene003608	MS.gene50662	PPI
MS.gene50662	MS.gene003583	PPI
MS.gene50662	MS.gene062951	PPI
MS.gene50662	MS.gene057801	PPI
MS.gene50662	MS.gene77527	PPI
MS.gene50662	MS.gene77524	PPI
MS.gene50662	MS.gene057795	PPI
MS.gene50662	MS.gene68162	PPI
MS.gene50662	MS.gene045363	PPI
MS.gene50662	MS.gene75675	PPI
MS.gene50662	MS.gene79887	PPI
MS.gene50662	MS.gene50652	PPI
MS.gene50662	MS.gene68761	PPI
MS.gene50662	MS.gene002380	PPI
MS.gene50662	MS.gene68165	PPI
MS.gene75677	MS.gene50662	PPI
MS.gene003625	MS.gene50662	PPI
MS.gene50662	MS.gene50677	PPI
MS.gene50662	MS.gene50684	PPI
MS.gene50662	MS.gene045366	PPI
MS.gene50662	MS.gene045362	PPI
MS.gene045361	MS.gene50662	PPI
MS.gene50662	MS.gene23372	PPI
MS.gene50662	MS.gene014668	PPI
MS.gene50662	MS.gene50765	PPI
MS.gene003591	MS.gene50662	PPI
MS.gene062979	MS.gene50662	PPI
MS.gene50662	MS.gene68759	PPI
MS.gene50662	MS.gene003586	PPI
MS.gene50662	MS.gene003627	PPI
MS.gene01358	MS.gene50662	PPI
MS.gene50662	MS.gene79891	PPI
MS.gene50662	MS.gene045367	PPI
MS.gene50662	MS.gene045364	PPI
MS.gene40326	MS.gene50662	PPI
MS.gene50662	MS.gene50687	PPI
MS.gene062952	MS.gene50662	PPI
MS.gene50676	MS.gene50662	PPI
MS.gene23370	MS.gene50662	PPI
MS.gene50766	MS.gene50662	PPI
MS.gene77529	MS.gene50662	PPI
MS.gene50662	MS.gene014669	PPI
MS.gene50662	MS.gene057808	PPI
MS.gene014667	MS.gene50662	PPI
MS.gene003594	MS.gene50662	PPI
MS.gene50665	MS.gene50662	PPI
MS.gene50662	MS.gene77526	PPI
MS.gene50662	MS.gene68163	PPI
MS.gene50662	MS.gene75315	PPI
MS.gene50662	MS.gene057818	PPI
MS.gene50662	MS.gene68164	PPI
MS.gene50763	MS.gene50662	PPI
MS.gene50662	MS.gene79889	PPI
MS.gene50662	MS.gene045368	PPI
MS.gene045365	MS.gene50662	PPI
MS.gene50662	MS.gene031200	PPI
MS.gene50662	MS.gene045360	PPI
MS.gene50662	MS.gene014222	PPI
MS.gene50662	MS.gene79888	PPI
MS.gene50659	MS.gene50662	PPI
MS.gene50662	MS.gene75676	PPI
MS.gene75316	MS.gene50662	PPI
MS.gene50662	MS.gene75678	PPI
MS.gene50662	MS.gene031199	PPI
MS.gene057790	MS.gene50662	PPI
MS.gene50662	MS.gene014223	PPI
MS.gene003624	MS.gene50662	PPI
MS.gene031201	MS.gene50662	PPI
MS.gene50662	MS.gene75673	PPI
MS.gene50662	MS.gene79885	PPI
MS.gene50662	MS.gene50764	PPI
MS.gene50662	MS.gene062963	PPI
MS.gene77523	MS.gene50662	PPI
MS.gene057778	MS.gene50662	PPI
MS.gene50662	MS.gene003577	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50662.t1	MTR_4g072730	79.121	91	19	0	1	91	1	91	1.25e-50	154
MS.gene50662.t1	MTR_4g072620	77.174	92	21	0	1	92	1	92	1.70e-49	151
MS.gene50662.t1	MTR_4g072480	77.174	92	21	0	1	92	1	92	3.04e-49	150
MS.gene50662.t1	MTR_4g072460	77.174	92	21	0	1	92	1	92	3.29e-49	150
MS.gene50662.t1	MTR_4g072680	77.174	92	21	0	1	92	1	92	5.04e-49	150
MS.gene50662.t1	MTR_4g072570	72.826	92	25	0	1	92	1	92	3.46e-46	143
MS.gene50662.t1	MTR_4g072660	75.000	92	18	1	1	92	1	87	1.34e-45	141
MS.gene50662.t1	MTR_4g072500	72.826	92	25	0	1	92	1	92	9.60e-45	139
MS.gene50662.t1	MTR_3g084180	68.478	92	29	0	1	92	1	92	4.61e-44	137
MS.gene50662.t1	MTR_3g084150	67.391	92	30	0	1	92	1	92	4.52e-43	135
MS.gene50662.t1	MTR_4g072530	71.111	90	26	0	1	90	1	90	4.17e-42	132
MS.gene50662.t1	MTR_8g461410	66.304	92	31	0	1	92	1	92	7.57e-42	132
MS.gene50662.t1	MTR_4g072310	65.591	93	31	1	1	92	1	93	7.72e-42	132
MS.gene50662.t1	MTR_4g072880	64.516	93	32	1	1	92	1	93	3.62e-41	130
MS.gene50662.t1	MTR_4g072640	67.778	90	29	0	1	90	1	90	1.39e-40	129
MS.gene50662.t1	MTR_3g110032	67.742	93	29	1	1	92	1	93	1.40e-40	129
MS.gene50662.t1	MTR_3g117630	68.889	90	28	0	1	90	1	90	2.65e-40	128
MS.gene50662.t1	MTR_4g072230	67.742	93	27	2	1	92	1	91	3.00e-40	128
MS.gene50662.t1	MTR_4g072270	64.516	93	32	1	1	92	1	93	1.53e-39	126
MS.gene50662.t1	MTR_4g072250	65.591	93	30	2	1	92	1	92	1.76e-39	126
MS.gene50662.t1	MTR_4g072240	61.538	91	34	1	1	90	1	91	1.79e-38	124
MS.gene50662.t1	MTR_3g084260	64.516	93	32	1	1	92	1	93	3.01e-38	123
MS.gene50662.t1	MTR_4g072550	79.452	73	15	0	20	92	31	103	4.62e-38	123
MS.gene50662.t1	MTR_3g084240	61.957	92	35	0	1	92	1	92	6.66e-38	122
MS.gene50662.t1	MTR_3g084250	63.043	92	34	0	1	92	1	92	7.35e-38	122
MS.gene50662.t1	MTR_4g072860	64.516	93	32	1	1	92	1	93	8.08e-38	122
MS.gene50662.t1	MTR_3g084170	61.957	92	35	0	1	92	1	92	1.09e-37	122
MS.gene50662.t1	MTR_4g072220	61.538	91	34	1	1	90	1	91	1.12e-37	122
MS.gene50662.t1	MTR_4g072370	65.556	90	29	1	1	90	1	88	1.27e-37	121
MS.gene50662.t1	MTR_4g072300	59.341	91	36	1	1	90	1	91	2.38e-37	120
MS.gene50662.t1	MTR_4g072330	62.222	90	32	1	1	90	1	88	3.11e-37	120
MS.gene50662.t1	MTR_4g072580	63.333	90	28	1	1	90	1	85	1.03e-36	119
MS.gene50662.t1	MTR_4g072870	60.440	91	35	1	1	90	1	91	1.98e-36	118
MS.gene50662.t1	MTR_4g050700	62.366	93	34	1	1	92	1	93	2.55e-36	118
MS.gene50662.t1	MTR_2g012410	62.366	93	34	1	1	92	1	93	2.55e-36	118
MS.gene50662.t1	MTR_3g110042	65.517	87	30	0	1	87	1	87	4.95e-36	117
MS.gene50662.t1	MTR_3g110038	61.111	90	35	0	1	90	1	90	6.51e-36	117
MS.gene50662.t1	MTR_5g008360	67.500	80	26	0	13	92	4	83	6.75e-36	117
MS.gene50662.t1	MTR_3g084220	60.870	92	36	0	1	92	1	92	1.03e-35	117
MS.gene50662.t1	MTR_4g072830	64.045	89	30	1	1	89	1	87	1.61e-35	116
MS.gene50662.t1	MTR_4g072890	58.242	91	37	1	1	90	1	91	4.97e-35	115
MS.gene50662.t1	MTR_4g072260	57.143	91	38	1	1	90	1	91	9.01e-35	114
MS.gene50662.t1	MTR_4g072470	61.111	90	30	1	1	90	1	85	9.51e-35	114
MS.gene50662.t1	MTR_4g072850	65.217	92	30	1	1	90	1	92	1.32e-34	114
MS.gene50662.t1	MTR_4g072740	61.111	90	30	1	1	90	1	85	5.09e-34	112
MS.gene50662.t1	MTR_4g072610	62.222	90	28	1	1	90	1	84	9.26e-34	111
MS.gene50662.t1	MTR_5g008370	69.333	75	23	0	18	92	18	92	2.76e-33	112
MS.gene50662.t1	MTR_4g072600	63.218	87	27	1	1	87	1	82	2.79e-33	110
MS.gene50662.t1	MTR_4g072490	58.889	90	32	1	1	90	1	85	2.91e-33	110
MS.gene50662.t1	MTR_3g084210	59.783	92	37	0	1	92	1	92	3.59e-33	110
MS.gene50662.t1	MTR_4g072630	57.778	90	34	1	1	90	1	86	6.88e-33	109
MS.gene50662.t1	MTR_3g084190	61.111	90	35	0	1	90	1	90	1.86e-32	108
MS.gene50662.t1	MTR_8g461420	58.696	92	38	0	1	92	1	92	3.40e-32	108
MS.gene50662.t1	MTR_4g072820	59.341	91	30	2	1	90	1	85	5.46e-32	107
MS.gene50662.t1	MTR_4g072465	55.556	90	36	1	1	90	1	86	7.43e-32	107
MS.gene50662.t1	MTR_4g072540	70.000	70	21	0	21	90	17	86	8.11e-32	107
MS.gene50662.t1	MTR_4g072650	58.889	90	31	1	1	90	1	84	1.20e-31	106
MS.gene50662.t1	MTR_3g110045	62.500	88	33	0	3	90	62	149	1.21e-31	108
MS.gene50662.t1	MTR_4g072940	68.056	72	23	0	21	92	53	124	1.37e-31	107
MS.gene50662.t1	MTR_3g084230	57.609	92	39	0	1	92	1	92	3.43e-31	105
MS.gene50662.t1	MTR_3g084160	58.889	90	37	0	1	90	1	90	7.30e-31	104
MS.gene50662.t1	MTR_4g072670	68.571	70	22	0	21	90	17	86	1.20e-30	103
MS.gene50662.t1	MTR_4g072720	67.143	70	23	0	21	90	17	86	1.76e-29	100
MS.gene50662.t1	MTR_4g072510	67.143	70	23	0	21	90	17	86	6.00e-29	99.8
MS.gene50662.t1	MTR_4g072380	57.143	91	33	2	1	90	1	86	1.94e-28	98.6
MS.gene50662.t1	MTR_4g072590	64.706	68	24	0	23	90	18	85	3.64e-28	97.8
MS.gene50662.t1	MTR_4g072690	66.176	68	23	0	23	90	18	85	1.11e-27	96.3
MS.gene50662.t1	MTR_5g008350	59.494	79	27	1	1	79	1	74	1.38e-27	95.9
MS.gene50662.t1	MTR_4g072290	51.613	93	30	2	1	92	1	79	3.21e-27	95.1
MS.gene50662.t1	MTR_4g072560	54.217	83	37	1	8	89	15	97	5.36e-27	95.1
MS.gene50662.t1	MTR_4g072980	50.538	93	40	2	1	92	8	95	6.04e-27	94.7
MS.gene50662.t1	MTR_4g072910	54.217	83	37	1	9	90	18	100	3.02e-26	93.2
MS.gene50662.t1	MTR_4g072450	63.235	68	25	0	23	90	18	85	5.61e-26	92.0
MS.gene50662.t1	MTR_4g072770	53.333	90	32	1	1	90	1	80	7.78e-26	91.7
MS.gene50662.t1	MTR_3g110035	62.687	67	25	0	26	92	72	138	1.05e-25	93.2
MS.gene50662.t1	MTR_3g117640	64.706	68	23	1	25	91	34	101	1.33e-25	91.7
MS.gene50662.t1	MTR_4g072750	61.111	72	26	1	1	70	1	72	6.16e-25	89.4
MS.gene50662.t1	MTR_3g084200	54.167	72	33	0	1	72	1	72	3.14e-23	85.9
MS.gene50662.t1	MTR_3g113310	56.923	65	27	1	23	87	40	103	3.47e-22	83.2
MS.gene50662.t1	MTR_6g077850	51.515	66	32	0	25	90	34	99	9.71e-22	81.6
MS.gene50662.t1	MTR_4g072280	58.209	67	27	1	1	66	1	67	3.68e-21	79.7
MS.gene50662.t1	MTR_4g072190	56.716	67	28	1	21	87	32	97	7.16e-21	79.7
MS.gene50662.t1	MTR_3g117610	58.462	65	26	1	23	87	43	106	7.78e-21	79.7
MS.gene50662.t1	MTR_8g461400	42.391	92	27	1	1	92	1	66	1.26e-17	70.5
MS.gene50662.t1	MTR_4g072930	51.471	68	29	1	27	90	43	110	2.03e-17	71.2
MS.gene50662.t1	MTR_8g076040	53.968	63	28	1	25	87	41	102	3.45e-17	70.5
MS.gene50662.t1	MTR_8g461210	45.455	88	45	2	6	91	16	102	4.40e-17	70.5
MS.gene50662.t1	MTR_5g021820	50.794	63	30	1	25	87	40	101	7.60e-17	69.3
MS.gene50662.t1	MTR_1g094750	41.176	85	45	2	5	85	26	109	4.38e-14	62.8
MS.gene50662.t1	MTR_4g072840	42.045	88	19	3	1	87	1	57	1.27e-13	60.1
MS.gene50662.t1	MTR_4g005310	45.455	66	35	1	21	85	58	123	5.34e-13	60.8
MS.gene50662.t1	MTR_2g066830	40.789	76	44	1	10	85	43	117	1.02e-12	59.7
MS.gene50662.t1	MTR_2g043960	43.478	69	38	1	17	85	68	135	1.41e-12	60.1
MS.gene50662.t1	MTR_4g072160	47.143	70	36	1	16	85	28	96	1.60e-12	59.3
MS.gene50662.t1	MTR_1g009150	44.872	78	38	2	12	85	54	130	1.76e-12	60.1
MS.gene50662.t1	MTR_1g024085	52.542	59	27	1	25	83	37	94	2.23e-12	58.9
MS.gene50662.t1	MTR_4g124750	46.032	63	33	1	25	87	69	130	4.73e-12	58.5
MS.gene50662.t1	MTR_1g061570	41.791	67	38	1	25	91	19	84	1.16e-11	56.6
MS.gene50662.t1	MTR_3g092220	39.080	87	43	4	1	83	12	92	1.86e-11	55.8
MS.gene50662.t1	MTR_7g118010	46.154	65	32	2	25	87	23	86	3.65e-11	55.1
MS.gene50662.t1	MTR_3g098970	35.000	80	47	2	15	92	43	119	4.15e-11	55.8
MS.gene50662.t1	MTR_1g063950	44.828	58	31	1	25	82	48	104	8.05e-11	54.7
MS.gene50662.t1	MTR_4g005320	45.902	61	32	1	25	85	70	129	8.40e-11	55.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50662.t1	AT4G38840	70.149	67	19	1	25	90	33	99	1.02e-29	102
MS.gene50662.t1	AT5G18020	51.648	91	42	2	1	90	1	90	1.54e-28	99.0
MS.gene50662.t1	AT3G03840	61.644	73	26	1	21	91	23	95	1.60e-28	99.0
MS.gene50662.t1	AT4G34770	53.933	89	39	1	5	91	15	103	8.31e-27	94.7
MS.gene50662.t1	AT5G18080	64.615	65	22	1	27	90	26	90	9.71e-27	94.4
MS.gene50662.t1	AT5G18030	49.425	87	41	2	7	90	2	88	1.01e-26	94.0
MS.gene50662.t1	AT5G18060	50.000	88	39	2	7	89	2	89	1.17e-26	94.0
MS.gene50662.t1	AT2G21200	52.941	85	38	2	7	89	2	86	2.23e-26	93.2
MS.gene50662.t1	AT5G18050	48.315	89	41	2	7	90	2	90	3.86e-26	92.8
MS.gene50662.t1	AT4G38825	54.667	75	33	1	17	90	15	89	1.21e-25	91.3
MS.gene50662.t1	AT4G34800	57.534	73	28	1	13	82	9	81	1.73e-25	91.3
MS.gene50662.t1	AT4G38850	54.762	84	34	2	9	88	4	87	2.19e-25	90.9
MS.gene50662.t1	AT5G18010	47.253	91	46	2	1	90	1	90	1.11e-24	89.0
MS.gene50662.t1	AT2G21210	54.795	73	31	1	20	90	23	95	2.26e-24	88.6
MS.gene50662.t1	AT3G03850	62.687	67	23	1	27	91	27	93	4.20e-24	87.8
MS.gene50662.t1	AT3G03820	51.724	87	36	2	8	91	13	96	1.60e-23	86.3
MS.gene50662.t1	AT2G21210	46.316	95	46	2	1	90	68	162	1.92e-23	88.2
MS.gene50662.t1	AT4G34810	56.098	82	29	2	8	82	28	109	2.10e-23	86.7
MS.gene50662.t1	AT4G34780	60.563	71	25	1	23	90	26	96	2.02e-22	84.0
MS.gene50662.t1	AT4G13790	50.602	83	34	2	8	88	13	90	3.86e-22	82.8
MS.gene50662.t1	AT3G03830	52.809	89	33	3	3	88	8	90	6.31e-22	82.0
MS.gene50662.t1	AT4G34760	56.923	65	27	1	23	87	41	104	3.34e-20	78.2
MS.gene50662.t1	AT1G75580	58.462	65	26	1	23	87	42	105	1.51e-19	76.6
MS.gene50662.t1	AT4G34790	58.462	65	25	1	25	87	39	103	1.53e-19	76.6
MS.gene50662.t1	AT2G21220	55.385	65	28	1	23	87	38	101	2.01e-19	76.3
MS.gene50662.t1	AT4G38860	53.846	65	30	0	23	87	38	102	2.51e-18	73.2
MS.gene50662.t1	AT2G16580	52.308	65	30	1	23	87	42	105	9.81e-17	69.3
MS.gene50662.t1	AT4G36110	52.308	65	30	1	25	89	40	103	4.84e-16	67.4
MS.gene50662.t1	AT1G19830	50.769	65	31	1	23	87	46	109	8.63e-16	67.0
MS.gene50662.t1	AT2G18010	50.769	65	31	1	25	89	48	111	2.19e-15	65.9
MS.gene50662.t1	AT3G20220	44.304	79	41	3	12	89	35	111	1.01e-14	64.3
MS.gene50662.t1	AT3G12830	43.750	80	43	2	6	85	32	109	3.51e-14	63.5
MS.gene50662.t1	AT5G10990	48.000	75	28	3	17	83	27	98	1.26e-13	62.4
MS.gene50662.t1	AT3G43120	38.554	83	50	1	3	85	57	138	4.53e-13	61.2
MS.gene50662.t1	AT3G03847	65.000	40	14	0	43	82	88	127	5.97e-13	60.1
MS.gene50662.t1	AT5G66260	46.970	66	34	1	25	90	34	98	6.01e-13	59.3
MS.gene50662.t1	AT1G75590	44.737	76	37	2	10	83	27	99	7.21e-13	60.5
MS.gene50662.t1	AT3G51200	45.614	57	30	1	26	82	41	96	2.21e-12	58.2
MS.gene50662.t1	AT2G46690	40.506	79	40	3	19	91	15	92	7.52e-12	57.4
MS.gene50662.t1	AT2G37030	43.284	67	37	1	16	82	40	105	9.62e-12	57.0
MS.gene50662.t1	AT4G34750	46.875	64	31	2	25	87	37	98	1.01e-11	57.4
MS.gene50662.t1	AT4G34750	46.875	64	31	2	25	87	37	98	1.01e-11	57.4
MS.gene50662.t1	AT1G19840	48.529	68	30	2	18	83	34	98	1.06e-11	57.4
MS.gene50662.t1	AT5G20810	47.541	61	31	1	25	85	79	138	1.11e-11	58.2
MS.gene50662.t1	AT5G20810	47.541	61	31	1	25	85	79	138	1.11e-11	57.8
MS.gene50662.t1	AT1G56150	41.667	84	42	3	6	85	17	97	5.65e-11	54.7

Find 20 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATATAACTTCTCGCTTTAA+TGG	0.140895	4.3:-33321391	MS.gene50662:CDS
CTCTTAGAATAGTGTGTAAA+TGG	0.286743	4.3:+33321632	None:intergenic
ATATAACTTCTCGCTTTAAT+GGG	0.299618	4.3:-33321390	MS.gene50662:CDS
AATCAAGCTGAGGAAGAGTT+TGG	0.355201	4.3:-33321469	MS.gene50662:CDS
TTCAACAAATCTTGAAATGA+TGG	0.381452	4.3:+33321490	None:intergenic
AAAATCTGTCAAAGTAAGAA+AGG	0.441806	4.3:-33321581	MS.gene50662:CDS
GACATCTTCGTTGCAAGGAA+TGG	0.508043	4.3:+33321420	None:intergenic
TAAAGCGAGAAGTTATATGT+TGG	0.535161	4.3:+33321395	None:intergenic
GGATATGTTGCAGTGTATGT+TGG	0.538211	4.3:-33321559	MS.gene50662:CDS
AAATCTGTCAAAGTAAGAAA+GGG	0.545163	4.3:-33321580	MS.gene50662:CDS
GGATATGATCATCCAATGGT+TGG	0.549482	4.3:-33321448	MS.gene50662:CDS
GGAATGGTGAGGCCAACCAT+TGG	0.572701	4.3:+33321436	None:intergenic
ATGTTGGAGAGGAACACAAA+TGG	0.583410	4.3:-33321543	MS.gene50662:CDS
TGGAAGACATCTTCGTTGCA+AGG	0.587446	4.3:+33321415	None:intergenic
TATAACTTCTCGCTTTAATG+GGG	0.622575	4.3:-33321389	MS.gene50662:CDS
GTTTGGATATGATCATCCAA+TGG	0.632664	4.3:-33321452	MS.gene50662:CDS
AGATTTGTTGAATCAAGCTG+AGG	0.640709	4.3:-33321479	MS.gene50662:CDS
TGTTGCAGTGTATGTTGGAG+AGG	0.641595	4.3:-33321554	MS.gene50662:CDS
CTTCGTTGCAAGGAATGGTG+AGG	0.665960	4.3:+33321425	None:intergenic
GTTCGTTTAAGTATGACACG+AGG	0.782180	4.3:+33321512	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAAATCTGTCAAAGTAAGAA+AGG	-	chr4.3:33321443-33321462	MS.gene50662:CDS	25.0%
!	AAATCTGTCAAAGTAAGAAA+GGG	-	chr4.3:33321444-33321463	MS.gene50662:CDS	25.0%
!	ATATAACTTCTCGCTTTAAT+GGG	-	chr4.3:33321634-33321653	MS.gene50662:CDS	25.0%
!	TTCAACAAATCTTGAAATGA+TGG	+	chr4.3:33321537-33321556	None:intergenic	25.0%
	CATATAACTTCTCGCTTTAA+TGG	-	chr4.3:33321633-33321652	MS.gene50662:CDS	30.0%
	CTCTTAGAATAGTGTGTAAA+TGG	+	chr4.3:33321395-33321414	None:intergenic	30.0%
	TAAAGCGAGAAGTTATATGT+TGG	+	chr4.3:33321632-33321651	None:intergenic	30.0%
	TATAACTTCTCGCTTTAATG+GGG	-	chr4.3:33321635-33321654	MS.gene50662:CDS	30.0%
	GTTTGGATATGATCATCCAA+TGG	-	chr4.3:33321572-33321591	MS.gene50662:CDS	35.0%
!!	AGATTTGTTGAATCAAGCTG+AGG	-	chr4.3:33321545-33321564	MS.gene50662:CDS	35.0%
	AATCAAGCTGAGGAAGAGTT+TGG	-	chr4.3:33321555-33321574	MS.gene50662:CDS	40.0%
	ATGTTGGAGAGGAACACAAA+TGG	-	chr4.3:33321481-33321500	MS.gene50662:CDS	40.0%
	GGATATGATCATCCAATGGT+TGG	-	chr4.3:33321576-33321595	MS.gene50662:CDS	40.0%
	GGATATGTTGCAGTGTATGT+TGG	-	chr4.3:33321465-33321484	MS.gene50662:CDS	40.0%
	GTTCGTTTAAGTATGACACG+AGG	+	chr4.3:33321515-33321534	None:intergenic	40.0%
	GACATCTTCGTTGCAAGGAA+TGG	+	chr4.3:33321607-33321626	None:intergenic	45.0%
	TGGAAGACATCTTCGTTGCA+AGG	+	chr4.3:33321612-33321631	None:intergenic	45.0%
	TGTTGCAGTGTATGTTGGAG+AGG	-	chr4.3:33321470-33321489	MS.gene50662:CDS	45.0%
	CTTCGTTGCAAGGAATGGTG+AGG	+	chr4.3:33321602-33321621	None:intergenic	50.0%
	GGAATGGTGAGGCCAACCAT+TGG	+	chr4.3:33321591-33321610	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	33321384	33321662	33321384	ID=MS.gene50662
chr4.3	mRNA	33321384	33321662	33321384	ID=MS.gene50662.t1;Parent=MS.gene50662
chr4.3	exon	33321384	33321662	33321384	ID=MS.gene50662.t1.exon1;Parent=MS.gene50662.t1
chr4.3	CDS	33321384	33321662	33321384	ID=cds.MS.gene50662.t1;Parent=MS.gene50662.t1

Gene Sequence	>MS.gene50662 ATGGGCTTCCATTTACACACTATTCTAAGAGCATCAGTAACTGCAAGACAAGCAAGAACAAAATCTGTCAAAGTAAGAAAGGGATATGTTGCAGTGTATGTTGGAGAGGAACACAAATGGTTTGTAGTCCTCGTGTCATACTTAAACGAACCATCATTTCAAGATTTGTTGAATCAAGCTGAGGAAGAGTTTGGATATGATCATCCAATGGTTGGCCTCACCATTCCTTGCAACGAAGATGTCTTCCAACATATAACTTCTCGCTTTAATGGGGTGTAA
Protein sequence	>MS.gene50662.t1 MGFHLHTILRASVTARQARTKSVKVRKGYVAVYVGEEHKWFVVLVSYLNEPSFQDLLNQAEEEFGYDHPMVGLTIPCNEDVFQHITSRFNGV