AlfalfaGEDB | MS.gene50666

M. sativa cultivar XinJiangDaYe / MS.gene50666

KEGG: K14488
GO: GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
PFAM: PF02519.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50666.t1	AES89342.1	96.7	91	3	0	1	91	1	91	5.00E-43	183.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50666.t1	P33083	76.7	90	21	0	1	90	1	90	1.0e-33	143.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50666.t1	G7JM99	96.7	91	3	0	1	91	1	91	3.6e-43	183.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene045360	MS.gene50666	PPI
MS.gene50654	MS.gene50666	PPI
MS.gene014669	MS.gene50666	PPI
MS.gene50666	MS.gene68163	PPI
MS.gene057818	MS.gene50666	PPI
MS.gene50652	MS.gene50666	PPI
MS.gene68162	MS.gene50666	PPI
MS.gene50687	MS.gene50666	PPI
MS.gene75677	MS.gene50666	PPI
MS.gene003625	MS.gene50666	PPI
MS.gene057795	MS.gene50666	PPI
MS.gene68165	MS.gene50666	PPI
MS.gene01764	MS.gene50666	PPI
MS.gene045361	MS.gene50666	PPI
MS.gene50666	MS.gene75678	PPI
MS.gene50675	MS.gene50666	PPI
MS.gene003591	MS.gene50666	PPI
MS.gene062979	MS.gene50666	PPI
MS.gene40325	MS.gene50666	PPI
MS.gene01358	MS.gene50666	PPI
MS.gene014668	MS.gene50666	PPI
MS.gene75675	MS.gene50666	PPI
MS.gene003583	MS.gene50666	PPI
MS.gene79888	MS.gene50666	PPI
MS.gene40326	MS.gene50666	PPI
MS.gene062952	MS.gene50666	PPI
MS.gene50666	MS.gene50764	PPI
MS.gene77527	MS.gene50666	PPI
MS.gene031199	MS.gene50666	PPI
MS.gene50676	MS.gene50666	PPI
MS.gene003614	MS.gene50666	PPI
MS.gene50666	MS.gene75672	PPI
MS.gene057797	MS.gene50666	PPI
MS.gene23370	MS.gene50666	PPI
MS.gene50766	MS.gene50666	PPI
MS.gene77529	MS.gene50666	PPI
MS.gene003594	MS.gene50666	PPI
MS.gene003596	MS.gene50666	PPI
MS.gene50665	MS.gene50666	PPI
MS.gene79889	MS.gene50666	PPI
MS.gene50677	MS.gene50666	PPI
MS.gene50666	MS.gene014670	PPI
MS.gene003624	MS.gene50666	PPI
MS.gene68759	MS.gene50666	PPI
MS.gene003586	MS.gene50666	PPI
MS.gene062988	MS.gene50666	PPI
MS.gene75673	MS.gene50666	PPI
MS.gene062963	MS.gene50666	PPI
MS.gene77528	MS.gene50666	PPI
MS.gene50666	MS.gene75679	PPI
MS.gene50763	MS.gene50666	PPI
MS.gene031198	MS.gene50666	PPI
MS.gene031200	MS.gene50666	PPI
MS.gene045365	MS.gene50666	PPI
MS.gene79885	MS.gene50666	PPI
MS.gene045364	MS.gene50666	PPI
MS.gene23372	MS.gene50666	PPI
MS.gene50659	MS.gene50666	PPI
MS.gene75316	MS.gene50666	PPI
MS.gene50666	MS.gene50684	PPI
MS.gene057790	MS.gene50666	PPI
MS.gene045363	MS.gene50666	PPI
MS.gene50666	MS.gene79886	PPI
MS.gene50666	MS.gene014671	PPI
MS.gene50666	MS.gene50765	PPI
MS.gene23371	MS.gene50666	PPI
MS.gene031201	MS.gene50666	PPI
MS.gene75314	MS.gene50666	PPI
MS.gene68761	MS.gene50666	PPI
MS.gene68760	MS.gene50666	PPI
MS.gene50666	MS.gene062974	PPI
MS.gene75676	MS.gene50666	PPI
MS.gene062951	MS.gene50666	PPI
MS.gene045362	MS.gene50666	PPI
MS.gene77523	MS.gene50666	PPI
MS.gene50666	MS.gene045367	PPI
MS.gene057778	MS.gene50666	PPI
MS.gene045366	MS.gene50666	PPI
MS.gene68164	MS.gene50666	PPI
MS.gene50666	MS.gene002380	PPI
MS.gene75674	MS.gene50666	PPI
MS.gene79890	MS.gene50666	PPI
MS.gene014667	MS.gene50666	PPI
MS.gene003615	MS.gene50666	PPI
MS.gene062973	MS.gene50666	PPI
MS.gene003602	MS.gene50666	PPI
MS.gene047517	MS.gene50666	PPI
MS.gene003627	MS.gene50666	PPI
MS.gene003577	MS.gene50666	PPI
MS.gene77524	MS.gene50666	PPI
MS.gene062984	MS.gene50666	PPI
MS.gene057801	MS.gene50666	PPI
MS.gene50666	MS.gene75315	PPI
MS.gene014223	MS.gene50666	PPI
MS.gene014222	MS.gene50666	PPI
MS.gene40327	MS.gene50666	PPI
MS.gene003587	MS.gene50666	PPI
MS.gene003590	MS.gene50666	PPI
MS.gene045368	MS.gene50666	PPI
MS.gene062943	MS.gene50666	PPI
MS.gene063112	MS.gene50666	PPI
MS.gene79891	MS.gene50666	PPI
MS.gene003608	MS.gene50666	PPI
MS.gene50666	MS.gene057808	PPI
MS.gene79887	MS.gene50666	PPI
MS.gene77526	MS.gene50666	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50666.t1	MTR_4g072730	96.703	91	3	0	1	91	1	91	5.41e-63	186
MS.gene50666.t1	MTR_4g072620	90.217	92	9	0	1	92	1	92	2.13e-59	176
MS.gene50666.t1	MTR_4g072480	89.130	92	10	0	1	92	1	92	1.81e-58	174
MS.gene50666.t1	MTR_4g072660	90.217	92	4	1	1	92	1	87	2.04e-56	169
MS.gene50666.t1	MTR_4g072680	85.870	92	13	0	1	92	1	92	4.59e-56	168
MS.gene50666.t1	MTR_4g072460	83.696	92	15	0	1	92	1	92	3.36e-54	163
MS.gene50666.t1	MTR_4g072500	81.522	92	17	0	1	92	1	92	1.62e-52	159
MS.gene50666.t1	MTR_4g072640	78.889	90	19	0	1	90	1	90	4.00e-50	153
MS.gene50666.t1	MTR_4g072570	77.174	92	21	0	1	92	1	92	2.58e-49	151
MS.gene50666.t1	MTR_4g072530	77.778	90	20	0	1	90	1	90	3.06e-49	150
MS.gene50666.t1	MTR_3g084150	72.826	92	25	0	1	92	1	92	5.59e-47	145
MS.gene50666.t1	MTR_4g072270	72.043	93	25	1	1	92	1	93	2.06e-46	144
MS.gene50666.t1	MTR_4g072310	70.968	93	26	1	1	92	1	93	4.84e-46	142
MS.gene50666.t1	MTR_3g084180	71.739	92	26	0	1	92	1	92	8.59e-46	142
MS.gene50666.t1	MTR_4g072880	69.892	93	27	1	1	92	1	93	1.18e-45	142
MS.gene50666.t1	MTR_8g461410	70.652	92	27	0	1	92	1	92	1.33e-45	142
MS.gene50666.t1	MTR_4g072860	73.118	93	24	1	1	92	1	93	4.25e-45	140
MS.gene50666.t1	MTR_4g072240	71.429	91	25	1	1	90	1	91	4.88e-45	140
MS.gene50666.t1	MTR_4g072230	70.968	93	24	2	1	92	1	91	3.40e-44	138
MS.gene50666.t1	MTR_4g072870	69.231	91	27	1	1	90	1	91	2.25e-43	136
MS.gene50666.t1	MTR_4g072250	72.043	93	24	2	1	92	1	92	2.26e-43	136
MS.gene50666.t1	MTR_3g084240	68.478	92	29	0	1	92	1	92	3.36e-43	135
MS.gene50666.t1	MTR_4g072220	66.667	93	30	1	1	92	1	93	4.41e-43	135
MS.gene50666.t1	MTR_4g072300	67.033	91	29	1	1	90	1	91	4.84e-43	135
MS.gene50666.t1	MTR_3g084170	68.478	92	29	0	1	92	1	92	6.28e-43	135
MS.gene50666.t1	MTR_3g117630	74.419	86	22	0	7	92	7	92	1.16e-42	134
MS.gene50666.t1	MTR_3g110042	72.414	87	24	0	1	87	1	87	7.58e-42	132
MS.gene50666.t1	MTR_4g072330	68.889	90	26	1	1	90	1	88	1.98e-41	131
MS.gene50666.t1	MTR_3g084220	67.391	92	30	0	1	92	1	92	4.73e-41	130
MS.gene50666.t1	MTR_3g110038	66.304	92	31	0	1	92	1	92	5.16e-41	130
MS.gene50666.t1	MTR_4g050700	66.667	93	30	1	1	92	1	93	1.27e-40	129
MS.gene50666.t1	MTR_2g012410	66.667	93	30	1	1	92	1	93	1.27e-40	129
MS.gene50666.t1	MTR_4g072370	67.778	90	27	1	1	90	1	88	1.46e-40	129
MS.gene50666.t1	MTR_3g084250	65.217	92	32	0	1	92	1	92	2.22e-40	128
MS.gene50666.t1	MTR_5g008360	76.250	80	19	0	13	92	4	83	2.32e-40	128
MS.gene50666.t1	MTR_3g110032	66.667	93	30	1	1	92	1	93	3.04e-40	128
MS.gene50666.t1	MTR_4g072890	63.736	91	32	1	1	90	1	91	3.27e-40	128
MS.gene50666.t1	MTR_4g072260	62.366	93	34	1	1	92	1	93	3.59e-40	128
MS.gene50666.t1	MTR_5g008370	79.730	74	15	0	18	91	18	91	8.09e-40	129
MS.gene50666.t1	MTR_4g072830	68.539	89	26	1	1	89	1	87	9.00e-40	127
MS.gene50666.t1	MTR_3g084210	66.304	92	31	0	1	92	1	92	2.48e-39	125
MS.gene50666.t1	MTR_4g072550	80.822	73	14	0	20	92	31	103	2.67e-39	126
MS.gene50666.t1	MTR_4g072630	66.667	90	26	1	1	90	1	86	3.72e-39	125
MS.gene50666.t1	MTR_4g072490	66.667	90	25	1	1	90	1	85	3.94e-39	125
MS.gene50666.t1	MTR_4g072580	65.556	90	26	1	1	90	1	85	6.92e-39	124
MS.gene50666.t1	MTR_4g072740	67.778	90	24	1	1	90	1	85	8.98e-39	124
MS.gene50666.t1	MTR_4g072600	66.667	90	25	1	1	90	1	85	1.21e-38	124
MS.gene50666.t1	MTR_4g072850	67.021	94	29	1	1	92	1	94	1.94e-38	124
MS.gene50666.t1	MTR_3g084260	63.441	93	33	1	1	92	1	93	4.37e-38	122
MS.gene50666.t1	MTR_3g084190	66.304	92	31	0	1	92	1	92	4.63e-38	122
MS.gene50666.t1	MTR_4g072540	67.778	90	25	1	1	90	1	86	6.73e-38	122
MS.gene50666.t1	MTR_8g461420	65.556	90	31	0	1	90	1	90	1.69e-37	121
MS.gene50666.t1	MTR_4g072470	65.556	90	26	1	1	90	1	85	2.05e-37	120
MS.gene50666.t1	MTR_3g084160	65.217	92	32	0	1	92	1	92	5.96e-37	120
MS.gene50666.t1	MTR_3g084230	65.556	90	31	0	1	90	1	90	7.03e-37	119
MS.gene50666.t1	MTR_4g072465	61.111	90	31	1	1	90	1	86	1.77e-36	118
MS.gene50666.t1	MTR_4g072670	65.556	90	27	1	1	90	1	86	7.28e-36	117
MS.gene50666.t1	MTR_4g072720	65.556	90	27	1	1	90	1	86	9.89e-36	116
MS.gene50666.t1	MTR_4g072590	64.444	90	27	1	1	90	1	85	1.06e-35	116
MS.gene50666.t1	MTR_4g072610	65.556	90	25	1	1	90	1	84	1.08e-35	116
MS.gene50666.t1	MTR_4g072510	65.556	90	27	1	1	90	1	86	5.01e-35	115
MS.gene50666.t1	MTR_4g072820	64.835	91	25	2	1	90	1	85	5.53e-35	114
MS.gene50666.t1	MTR_3g110045	64.516	93	31	1	2	92	59	151	6.42e-35	116
MS.gene50666.t1	MTR_4g072940	73.611	72	19	0	21	92	53	124	9.08e-35	115
MS.gene50666.t1	MTR_4g072380	62.222	90	30	1	1	90	1	86	1.86e-34	113
MS.gene50666.t1	MTR_4g072650	61.111	90	29	1	1	90	1	84	3.30e-33	110
MS.gene50666.t1	MTR_4g072690	63.333	90	28	1	1	90	1	85	4.36e-32	107
MS.gene50666.t1	MTR_4g072290	56.989	93	25	2	1	92	1	79	1.97e-31	105
MS.gene50666.t1	MTR_5g008350	63.291	79	24	1	1	79	1	74	2.40e-31	105
MS.gene50666.t1	MTR_4g072450	60.000	90	31	1	1	90	1	85	3.61e-30	102
MS.gene50666.t1	MTR_4g072770	57.778	90	28	1	1	90	1	80	4.20e-30	102
MS.gene50666.t1	MTR_4g072980	53.763	93	37	2	1	92	8	95	3.80e-29	100
MS.gene50666.t1	MTR_4g072750	57.647	85	32	2	1	81	1	85	1.10e-28	99.0
MS.gene50666.t1	MTR_3g084200	61.111	72	28	0	1	72	1	72	1.18e-28	99.4
MS.gene50666.t1	MTR_3g117640	69.118	68	20	1	25	91	34	101	1.22e-28	99.4
MS.gene50666.t1	MTR_4g072910	64.179	67	24	0	24	90	34	100	1.22e-28	99.4
MS.gene50666.t1	MTR_4g072560	53.608	97	37	3	1	89	1	97	2.83e-28	98.2
MS.gene50666.t1	MTR_3g110035	66.667	66	22	0	27	92	73	138	1.48e-27	97.8
MS.gene50666.t1	MTR_4g072280	57.333	75	31	1	1	74	1	75	2.59e-26	92.8
MS.gene50666.t1	MTR_6g077850	55.224	67	30	0	24	90	33	99	1.95e-25	91.3
MS.gene50666.t1	MTR_3g113310	57.353	68	28	1	23	90	40	106	4.29e-25	90.5
MS.gene50666.t1	MTR_4g072190	52.326	86	39	2	5	90	17	100	3.77e-24	87.8
MS.gene50666.t1	MTR_3g117610	59.701	67	26	1	23	89	43	108	3.97e-24	88.2
MS.gene50666.t1	MTR_8g076040	48.750	80	40	1	11	90	27	105	2.96e-21	80.9
MS.gene50666.t1	MTR_4g072930	51.852	81	33	2	16	90	30	110	5.40e-21	80.1
MS.gene50666.t1	MTR_5g021820	52.239	67	31	1	24	90	39	104	7.79e-21	79.7
MS.gene50666.t1	MTR_8g461400	46.739	92	23	1	1	92	1	66	7.59e-20	75.9
MS.gene50666.t1	MTR_2g043960	46.667	75	39	1	15	89	66	139	2.22e-18	75.1
MS.gene50666.t1	MTR_4g124750	50.000	66	32	1	24	89	68	132	7.79e-18	73.2
MS.gene50666.t1	MTR_8g461210	41.860	86	49	1	4	89	14	98	1.21e-16	69.3
MS.gene50666.t1	MTR_1g024085	46.988	83	40	2	4	83	13	94	1.64e-16	69.7
MS.gene50666.t1	MTR_4g072840	46.154	91	17	3	1	90	1	60	4.57e-16	66.2
MS.gene50666.t1	MTR_4g005310	46.970	66	34	1	21	85	58	123	1.07e-15	67.8
MS.gene50666.t1	MTR_1g009150	52.459	61	28	1	25	85	71	130	4.84e-15	66.6
MS.gene50666.t1	MTR_4g072160	50.000	70	32	2	17	85	29	96	1.85e-14	64.3
MS.gene50666.t1	MTR_1g094750	46.667	75	36	2	14	85	36	109	1.95e-14	63.5
MS.gene50666.t1	MTR_7g118010	48.529	68	32	2	25	90	23	89	1.95e-14	63.5
MS.gene50666.t1	MTR_0388s0020	43.836	73	37	2	20	89	70	141	5.68e-14	63.2
MS.gene50666.t1	MTR_3g109160	49.254	67	33	1	25	91	69	134	5.75e-14	63.5
MS.gene50666.t1	MTR_4g025830	42.308	78	41	2	13	87	67	143	8.40e-14	63.2
MS.gene50666.t1	MTR_1g061570	45.588	68	36	1	24	91	18	84	1.47e-13	61.6
MS.gene50666.t1	MTR_8g026730	45.588	68	34	2	25	90	27	93	2.10e-13	60.8
MS.gene50666.t1	MTR_4g005320	47.541	61	31	1	25	85	70	129	2.11e-13	62.0
MS.gene50666.t1	MTR_3g092220	38.824	85	46	3	1	83	12	92	2.12e-13	60.8
MS.gene50666.t1	MTR_2g066830	37.079	89	49	2	2	90	41	122	1.66e-12	59.3
MS.gene50666.t1	MTR_1g059770	44.776	67	33	2	28	91	52	117	2.33e-12	58.5
MS.gene50666.t1	MTR_3g098970	35.897	78	49	1	15	92	43	119	4.06e-12	58.5
MS.gene50666.t1	MTR_3g097120	41.333	75	35	2	19	85	3	76	9.47e-12	56.6
MS.gene50666.t1	MTR_7g104940	41.935	62	35	1	28	89	53	113	1.37e-11	56.6
MS.gene50666.t1	MTR_1g063950	40.000	65	38	1	24	88	47	110	2.42e-11	55.8
MS.gene50666.t1	MTR_7g405740	44.068	59	32	1	27	85	69	126	8.22e-11	55.5
MS.gene50666.t1	MTR_7g104950	38.710	62	37	1	28	89	53	113	8.68e-11	54.7
MS.gene50666.t1	MTR_7g101740	42.188	64	36	1	24	87	39	101	9.04e-11	54.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50666.t1	AT4G38840	57.576	99	33	3	1	90	1	99	5.42e-32	107
MS.gene50666.t1	AT4G34770	57.778	90	36	1	4	91	14	103	7.60e-31	105
MS.gene50666.t1	AT4G38825	56.627	83	35	1	9	90	7	89	3.04e-29	100
MS.gene50666.t1	AT5G18020	53.409	88	39	2	1	87	1	87	4.19e-29	100
MS.gene50666.t1	AT5G18080	69.231	65	19	1	27	90	26	90	7.19e-29	99.8
MS.gene50666.t1	AT2G21210	51.579	95	41	2	1	90	1	95	1.25e-28	99.4
MS.gene50666.t1	AT5G18060	67.692	65	20	1	27	90	26	90	1.43e-28	99.0
MS.gene50666.t1	AT3G03840	56.044	91	35	2	3	91	8	95	1.76e-28	99.0
MS.gene50666.t1	AT4G34800	55.294	85	31	2	1	82	1	81	1.96e-28	98.6
MS.gene50666.t1	AT2G21200	64.789	71	24	1	20	89	16	86	1.97e-28	98.2
MS.gene50666.t1	AT3G03820	58.621	87	30	2	8	91	13	96	2.81e-28	98.2
MS.gene50666.t1	AT5G18030	59.459	74	29	1	18	90	15	88	3.22e-28	97.8
MS.gene50666.t1	AT5G18050	66.154	65	21	1	27	90	26	90	6.02e-28	97.4
MS.gene50666.t1	AT3G03850	59.783	92	28	3	3	91	8	93	6.41e-28	97.4
MS.gene50666.t1	AT2G21210	51.579	95	41	2	1	90	68	162	6.77e-28	99.4
MS.gene50666.t1	AT4G38850	62.500	72	24	1	19	87	15	86	3.41e-27	95.5
MS.gene50666.t1	AT5G18010	50.549	91	43	2	1	90	1	90	6.84e-27	94.7
MS.gene50666.t1	AT4G34810	66.129	62	17	1	25	82	48	109	3.78e-26	93.6
MS.gene50666.t1	AT3G03830	54.545	88	31	2	3	87	8	89	6.72e-25	89.7
MS.gene50666.t1	AT4G13790	57.143	70	28	1	20	87	20	89	2.72e-24	88.2
MS.gene50666.t1	AT4G38860	58.824	68	28	0	23	90	38	105	6.22e-24	87.4
MS.gene50666.t1	AT1G75580	60.294	68	26	1	23	90	42	108	5.09e-23	85.5
MS.gene50666.t1	AT2G21220	60.000	65	25	1	23	87	38	101	5.56e-23	85.1
MS.gene50666.t1	AT4G34790	60.000	65	24	1	25	87	39	103	6.20e-23	85.1
MS.gene50666.t1	AT4G34760	57.353	68	28	1	23	90	41	107	6.37e-23	85.1
MS.gene50666.t1	AT4G34780	59.155	71	26	1	23	90	26	96	1.18e-22	84.3
MS.gene50666.t1	AT2G16580	52.941	68	31	1	23	90	42	108	1.51e-19	76.6
MS.gene50666.t1	AT3G43120	41.379	87	50	1	5	91	59	144	2.80e-19	77.4
MS.gene50666.t1	AT1G19830	56.338	71	29	2	20	89	43	112	2.91e-19	75.9
MS.gene50666.t1	AT4G36110	54.688	64	28	1	24	87	39	101	1.02e-18	74.3
MS.gene50666.t1	AT2G18010	53.125	64	29	1	24	87	47	109	5.88e-18	72.4
MS.gene50666.t1	AT5G20810	50.000	66	32	1	24	89	78	142	1.97e-17	72.8
MS.gene50666.t1	AT5G20810	46.575	73	38	1	17	89	71	142	2.26e-17	73.2
MS.gene50666.t1	AT3G20220	51.613	62	28	2	25	85	47	107	8.96e-17	69.7
MS.gene50666.t1	AT5G66260	46.914	81	37	2	15	90	19	98	1.16e-16	68.9
MS.gene50666.t1	AT1G75590	44.706	85	41	2	4	83	16	99	5.38e-16	68.6
MS.gene50666.t1	AT5G10990	50.725	69	33	1	15	83	31	98	2.18e-15	67.0
MS.gene50666.t1	AT3G12830	41.379	87	49	2	4	90	30	114	4.57e-15	65.9
MS.gene50666.t1	AT3G51200	50.000	56	27	1	27	82	42	96	1.16e-14	63.9
MS.gene50666.t1	AT1G19840	50.725	69	33	1	15	83	31	98	1.80e-14	64.7
MS.gene50666.t1	AT3G61900	43.590	78	40	2	16	90	21	97	5.70e-14	63.2
MS.gene50666.t1	AT5G53590	45.833	72	33	2	24	90	45	115	8.37e-14	62.8
MS.gene50666.t1	AT4G34750	46.875	64	33	1	24	87	36	98	9.53e-14	62.8
MS.gene50666.t1	AT4G34750	46.875	64	33	1	24	87	36	98	9.53e-14	62.8
MS.gene50666.t1	AT3G60690	43.056	72	37	2	19	87	82	152	1.89e-13	62.4
MS.gene50666.t1	AT2G46690	41.892	74	39	2	21	91	20	92	2.44e-13	60.8
MS.gene50666.t1	AT3G09870	48.438	64	30	2	24	85	44	106	1.25e-12	58.9
MS.gene50666.t1	AT2G45210	40.845	71	38	2	20	87	74	143	1.36e-12	60.1
MS.gene50666.t1	AT2G37030	41.538	65	37	1	24	88	48	111	2.59e-12	58.5
MS.gene50666.t1	AT1G56150	43.421	76	41	2	15	90	29	102	3.49e-12	57.8
MS.gene50666.t1	AT4G00880	44.928	69	35	2	24	90	27	94	5.87e-12	57.4
MS.gene50666.t1	AT3G03847	42.105	76	41	3	8	82	54	127	7.00e-12	57.4
MS.gene50666.t1	AT4G09530	41.176	68	37	2	27	91	30	97	7.39e-12	56.6
MS.gene50666.t1	AT4G31320	45.902	61	32	1	25	85	80	139	1.33e-11	57.8
MS.gene50666.t1	AT1G16510	43.939	66	36	1	25	90	54	118	1.59e-11	57.0
MS.gene50666.t1	AT2G24400	47.541	61	31	1	25	85	64	123	2.23e-11	57.0

Find 22 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATATAACTTCTTGCTTGAA+TGG	0.302470	4.3:+33328763	MS.gene50666:CDS
ATTTCAAGACTTGTTGAATC+AGG	0.319811	4.3:+33328669	MS.gene50666:CDS
AATCAGGCTGAAGAAGAGTT+TGG	0.367604	4.3:+33328685	MS.gene50666:CDS
AAATCTGTAGAAGTGATAAA+AGG	0.369929	4.3:+33328574	MS.gene50666:CDS
CGTTTACACACTATTCTAAA+GGG	0.375968	4.3:+33328523	MS.gene50666:CDS
GGTTGGTTTAAGTATGAAAC+AGG	0.401861	4.3:-33328643	None:intergenic
CCGTTTACACACTATTCTAA+AGG	0.405819	4.3:+33328522	MS.gene50666:CDS
TTCAACAAGTCTTGAAATGA+AGG	0.413456	4.3:-33328664	None:intergenic
ATATAACTTCTTGCTTGAAT+GGG	0.446395	4.3:+33328764	MS.gene50666:CDS
CCTTTAGAATAGTGTGTAAA+CGG	0.450829	4.3:-33328522	None:intergenic
ACAAGTCTTGAAATGAAGGT+TGG	0.490149	4.3:-33328660	None:intergenic
TCAAGCAAGAAGTTATATGT+TGG	0.522465	4.3:-33328759	None:intergenic
GGCTATGTTGCAGTGTATGT+TGG	0.544627	4.3:+33328595	MS.gene50666:CDS
TTTGGATATGATCATCCAAT+GGG	0.545525	4.3:+33328703	MS.gene50666:CDS
GACATCTTCGGAGCAAGGAA+TGG	0.562238	4.3:-33328734	None:intergenic
GGAATGGTGAGGCCACCCAT+TGG	0.562484	4.3:-33328718	None:intergenic
TATATGTTGGAAGACATCTT+CGG	0.572640	4.3:-33328746	None:intergenic
GTTTGGATATGATCATCCAA+TGG	0.573812	4.3:+33328702	MS.gene50666:CDS
TGGAAGACATCTTCGGAGCA+AGG	0.586129	4.3:-33328739	None:intergenic
GGATATGATCATCCAATGGG+TGG	0.648071	4.3:+33328706	MS.gene50666:CDS
CTTCGGAGCAAGGAATGGTG+AGG	0.674250	4.3:-33328729	None:intergenic
ATGTTGGAGAGAAACTGACG+CGG	0.811075	4.3:+33328611	MS.gene50666:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAATCTGTAGAAGTGATAAA+AGG	+	chr4.3:33328574-33328593	MS.gene50666:CDS	25.0%
!	ATATAACTTCTTGCTTGAAT+GGG	+	chr4.3:33328764-33328783	MS.gene50666:CDS	25.0%
	CATATAACTTCTTGCTTGAA+TGG	+	chr4.3:33328763-33328782	MS.gene50666:CDS	30.0%
	CGTTTACACACTATTCTAAA+GGG	+	chr4.3:33328523-33328542	MS.gene50666:CDS	30.0%
	TATATGTTGGAAGACATCTT+CGG	-	chr4.3:33328749-33328768	None:intergenic	30.0%
	TCAAGCAAGAAGTTATATGT+TGG	-	chr4.3:33328762-33328781	None:intergenic	30.0%
	TTCAACAAGTCTTGAAATGA+AGG	-	chr4.3:33328667-33328686	None:intergenic	30.0%
	TTTGGATATGATCATCCAAT+GGG	+	chr4.3:33328703-33328722	MS.gene50666:CDS	30.0%
!!	ATTTCAAGACTTGTTGAATC+AGG	+	chr4.3:33328669-33328688	MS.gene50666:CDS	30.0%
!!	CCTTTAGAATAGTGTGTAAA+CGG	-	chr4.3:33328525-33328544	None:intergenic	30.0%
	ACAAGTCTTGAAATGAAGGT+TGG	-	chr4.3:33328663-33328682	None:intergenic	35.0%
	CCGTTTACACACTATTCTAA+AGG	+	chr4.3:33328522-33328541	MS.gene50666:CDS	35.0%
	GTTTGGATATGATCATCCAA+TGG	+	chr4.3:33328702-33328721	MS.gene50666:CDS	35.0%
!!	GGTTGGTTTAAGTATGAAAC+AGG	-	chr4.3:33328646-33328665	None:intergenic	35.0%
	AATCAGGCTGAAGAAGAGTT+TGG	+	chr4.3:33328685-33328704	MS.gene50666:CDS	40.0%
	ATGTTGGAGAGAAACTGACG+CGG	+	chr4.3:33328611-33328630	MS.gene50666:CDS	45.0%
	GGATATGATCATCCAATGGG+TGG	+	chr4.3:33328706-33328725	MS.gene50666:CDS	45.0%
	GGCTATGTTGCAGTGTATGT+TGG	+	chr4.3:33328595-33328614	MS.gene50666:CDS	45.0%
	GACATCTTCGGAGCAAGGAA+TGG	-	chr4.3:33328737-33328756	None:intergenic	50.0%
	TGGAAGACATCTTCGGAGCA+AGG	-	chr4.3:33328742-33328761	None:intergenic	50.0%
	CTTCGGAGCAAGGAATGGTG+AGG	-	chr4.3:33328732-33328751	None:intergenic	55.0%
	GGAATGGTGAGGCCACCCAT+TGG	-	chr4.3:33328721-33328740	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	33328514	33328792	33328514	ID=MS.gene50666
chr4.3	mRNA	33328514	33328792	33328514	ID=MS.gene50666.t1;Parent=MS.gene50666
chr4.3	exon	33328514	33328792	33328514	ID=MS.gene50666.t1.exon1;Parent=MS.gene50666.t1
chr4.3	CDS	33328514	33328792	33328514	ID=cds.MS.gene50666.t1;Parent=MS.gene50666.t1

Gene Sequence	>MS.gene50666 ATGGGTTTCCGTTTACACACTATTCTAAAGGGTTCAGTAACTTCTAGTCAAGCAAAATCAAAATCTGTAGAAGTGATAAAAGGCTATGTTGCAGTGTATGTTGGAGAGAAACTGACGCGGTTTGTAGTTCCTGTTTCATACTTAAACCAACCTTCATTTCAAGACTTGTTGAATCAGGCTGAAGAAGAGTTTGGATATGATCATCCAATGGGTGGCCTCACCATTCCTTGCTCCGAAGATGTCTTCCAACATATAACTTCTTGCTTGAATGGGCTATGA
Protein sequence	>MS.gene50666.t1 MGFRLHTILKGSVTSSQAKSKSVEVIKGYVAVYVGEKLTRFVVPVSYLNQPSFQDLLNQAEEEFGYDHPMGGLTIPCSEDVFQHITSCLNGL