AlfalfaGEDB | MS.gene50668

M. sativa cultivar XinJiangDaYe / MS.gene50668

KEGG: K14488
GO: GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
PFAM: PF02519.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50668.t1	AES89321.1	88.4	86	10	0	1	86	1	86	6.50E-37	162.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50668.t1	P33080	65.6	90	27	1	1	86	1	90	3.3e-29	128.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50668.t1	G7JL46	88.4	86	10	0	1	86	1	86	4.7e-37	162.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene50668	MS.gene062951	PPI
MS.gene045368	MS.gene50668	PPI
MS.gene50668	MS.gene68162	PPI
MS.gene063112	MS.gene50668	PPI
MS.gene50668	MS.gene045367	PPI
MS.gene50668	MS.gene045363	PPI
MS.gene50668	MS.gene79887	PPI
MS.gene79890	MS.gene50668	PPI
MS.gene003615	MS.gene50668	PPI
MS.gene50668	MS.gene50652	PPI
MS.gene062973	MS.gene50668	PPI
MS.gene50668	MS.gene002380	PPI
MS.gene77526	MS.gene50668	PPI
MS.gene047517	MS.gene50668	PPI
MS.gene045360	MS.gene50668	PPI
MS.gene50668	MS.gene50684	PPI
MS.gene057801	MS.gene50668	PPI
MS.gene014223	MS.gene50668	PPI
MS.gene50687	MS.gene50668	PPI
MS.gene40327	MS.gene50668	PPI
MS.gene75677	MS.gene50668	PPI
MS.gene50668	MS.gene014668	PPI
MS.gene057795	MS.gene50668	PPI
MS.gene50668	MS.gene68164	PPI
MS.gene68165	MS.gene50668	PPI
MS.gene045361	MS.gene50668	PPI
MS.gene50675	MS.gene50668	PPI
MS.gene003591	MS.gene50668	PPI
MS.gene062979	MS.gene50668	PPI
MS.gene50668	MS.gene79891	PPI
MS.gene003590	MS.gene50668	PPI
MS.gene062943	MS.gene50668	PPI
MS.gene003608	MS.gene50668	PPI
MS.gene40326	MS.gene50668	PPI
MS.gene50668	MS.gene014669	PPI
MS.gene50668	MS.gene057808	PPI
MS.gene77527	MS.gene50668	PPI
MS.gene50676	MS.gene50668	PPI
MS.gene003614	MS.gene50668	PPI
MS.gene057818	MS.gene50668	PPI
MS.gene50766	MS.gene50668	PPI
MS.gene003594	MS.gene50668	PPI
MS.gene50668	MS.gene68163	PPI
MS.gene79889	MS.gene50668	PPI
MS.gene50677	MS.gene50668	PPI
MS.gene003625	MS.gene50668	PPI
MS.gene68759	MS.gene50668	PPI
MS.gene50668	MS.gene75315	PPI
MS.gene50668	MS.gene014222	PPI
MS.gene50668	MS.gene79888	PPI
MS.gene50763	MS.gene50668	PPI
MS.gene50668	MS.gene75678	PPI
MS.gene40325	MS.gene50668	PPI
MS.gene01358	MS.gene50668	PPI
MS.gene50659	MS.gene50668	PPI
MS.gene75675	MS.gene50668	PPI
MS.gene50668	MS.gene79885	PPI
MS.gene003583	MS.gene50668	PPI
MS.gene057790	MS.gene50668	PPI
MS.gene062952	MS.gene50668	PPI
MS.gene003624	MS.gene50668	PPI
MS.gene031199	MS.gene50668	PPI
MS.gene50668	MS.gene50764	PPI
MS.gene75314	MS.gene50668	PPI
MS.gene057797	MS.gene50668	PPI
MS.gene23370	MS.gene50668	PPI
MS.gene77529	MS.gene50668	PPI
MS.gene68761	MS.gene50668	PPI
MS.gene014667	MS.gene50668	PPI
MS.gene68760	MS.gene50668	PPI
MS.gene003596	MS.gene50668	PPI
MS.gene75676	MS.gene50668	PPI
MS.gene50665	MS.gene50668	PPI
MS.gene003586	MS.gene50668	PPI
MS.gene057778	MS.gene50668	PPI
MS.gene062988	MS.gene50668	PPI
MS.gene75673	MS.gene50668	PPI
MS.gene50668	MS.gene014670	PPI
MS.gene045366	MS.gene50668	PPI
MS.gene062963	MS.gene50668	PPI
MS.gene77528	MS.gene50668	PPI
MS.gene031198	MS.gene50668	PPI
MS.gene75674	MS.gene50668	PPI
MS.gene031200	MS.gene50668	PPI
MS.gene045365	MS.gene50668	PPI
MS.gene50668	MS.gene01764	PPI
MS.gene50668	MS.gene75679	PPI
MS.gene045364	MS.gene50668	PPI
MS.gene003602	MS.gene50668	PPI
MS.gene23372	MS.gene50668	PPI
MS.gene75316	MS.gene50668	PPI
MS.gene003627	MS.gene50668	PPI
MS.gene003577	MS.gene50668	PPI
MS.gene77524	MS.gene50668	PPI
MS.gene062984	MS.gene50668	PPI
MS.gene23371	MS.gene50668	PPI
MS.gene031201	MS.gene50668	PPI
MS.gene50668	MS.gene79886	PPI
MS.gene50668	MS.gene014671	PPI
MS.gene50668	MS.gene50765	PPI
MS.gene50668	MS.gene75672	PPI
MS.gene045362	MS.gene50668	PPI
MS.gene50668	MS.gene50654	PPI
MS.gene50668	MS.gene062974	PPI
MS.gene77523	MS.gene50668	PPI
MS.gene003587	MS.gene50668	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50668.t1	MTR_4g072630	86.047	86	12	0	1	86	1	86	1.72e-53	161
MS.gene50668.t1	MTR_4g072740	86.047	86	11	1	1	86	1	85	1.23e-52	159
MS.gene50668.t1	MTR_4g072490	84.884	86	12	1	1	86	1	85	3.87e-51	155
MS.gene50668.t1	MTR_4g072465	82.558	86	15	0	1	86	1	86	7.37e-51	154
MS.gene50668.t1	MTR_4g072580	81.395	86	15	1	1	86	1	85	4.05e-48	147
MS.gene50668.t1	MTR_4g072470	81.395	86	15	1	1	86	1	85	5.54e-48	147
MS.gene50668.t1	MTR_4g072370	78.409	88	17	1	1	86	1	88	1.19e-47	146
MS.gene50668.t1	MTR_4g072830	78.161	87	17	1	1	85	1	87	7.52e-47	144
MS.gene50668.t1	MTR_4g072670	86.047	86	12	0	1	86	1	86	1.33e-46	144
MS.gene50668.t1	MTR_4g072510	88.372	86	10	0	1	86	1	86	1.35e-46	144
MS.gene50668.t1	MTR_4g072540	86.047	86	12	0	1	86	1	86	3.79e-45	140
MS.gene50668.t1	MTR_4g072720	86.047	86	12	0	1	86	1	86	4.42e-45	140
MS.gene50668.t1	MTR_4g072610	77.907	86	17	1	1	86	1	84	1.21e-44	139
MS.gene50668.t1	MTR_4g072650	77.907	86	17	1	1	86	1	84	2.20e-44	138
MS.gene50668.t1	MTR_4g072600	75.581	86	20	1	1	86	1	85	6.35e-44	137
MS.gene50668.t1	MTR_3g084150	70.000	90	23	1	1	86	1	90	1.25e-43	136
MS.gene50668.t1	MTR_4g072330	71.910	89	21	2	1	86	1	88	5.77e-43	134
MS.gene50668.t1	MTR_8g461410	68.889	90	24	1	1	86	1	90	7.13e-43	134
MS.gene50668.t1	MTR_3g084180	68.889	90	24	1	1	86	1	90	9.92e-43	134
MS.gene50668.t1	MTR_3g084170	68.889	90	24	1	1	86	1	90	4.61e-42	132
MS.gene50668.t1	MTR_3g110038	66.667	90	26	1	1	86	1	90	4.00e-41	130
MS.gene50668.t1	MTR_3g084240	67.778	90	25	1	1	86	1	90	5.68e-41	129
MS.gene50668.t1	MTR_4g072880	68.132	91	24	1	1	86	1	91	6.25e-41	129
MS.gene50668.t1	MTR_4g072310	68.132	91	24	1	1	86	1	91	6.53e-41	129
MS.gene50668.t1	MTR_4g072870	66.304	92	24	2	1	86	1	91	2.83e-40	128
MS.gene50668.t1	MTR_4g072240	63.736	91	28	1	1	86	1	91	4.49e-40	127
MS.gene50668.t1	MTR_5g008350	77.333	75	16	1	1	75	1	74	5.77e-40	127
MS.gene50668.t1	MTR_4g072590	75.581	86	20	1	1	86	1	85	7.19e-40	127
MS.gene50668.t1	MTR_3g084220	65.556	90	27	1	1	86	1	90	8.17e-40	127
MS.gene50668.t1	MTR_3g110032	64.835	91	27	1	1	86	1	91	1.08e-39	126
MS.gene50668.t1	MTR_4g050700	65.934	91	26	1	1	86	1	91	1.63e-39	126
MS.gene50668.t1	MTR_2g012410	65.934	91	26	1	1	86	1	91	1.63e-39	126
MS.gene50668.t1	MTR_3g084250	67.778	90	25	1	1	86	1	90	2.12e-39	125
MS.gene50668.t1	MTR_4g072270	65.934	91	26	1	1	86	1	91	5.74e-39	124
MS.gene50668.t1	MTR_4g072250	69.231	91	22	2	1	86	1	90	6.08e-39	124
MS.gene50668.t1	MTR_4g072480	63.333	90	29	1	1	86	1	90	6.29e-39	124
MS.gene50668.t1	MTR_4g072300	61.538	91	30	1	1	86	1	91	6.59e-39	124
MS.gene50668.t1	MTR_4g072220	62.637	91	29	1	1	86	1	91	9.40e-39	124
MS.gene50668.t1	MTR_4g072690	73.256	86	22	1	1	86	1	85	1.53e-38	123
MS.gene50668.t1	MTR_4g072890	61.538	91	30	1	1	86	1	91	1.79e-38	123
MS.gene50668.t1	MTR_4g072380	70.115	87	24	2	1	86	1	86	2.66e-38	123
MS.gene50668.t1	MTR_3g084260	63.736	91	28	1	1	86	1	91	3.25e-38	122
MS.gene50668.t1	MTR_4g072850	67.391	92	24	1	1	86	1	92	3.65e-38	122
MS.gene50668.t1	MTR_4g072450	73.256	86	22	1	1	86	1	85	5.02e-38	122
MS.gene50668.t1	MTR_4g072820	71.264	87	22	2	1	86	1	85	6.95e-38	122
MS.gene50668.t1	MTR_4g072260	60.440	91	31	1	1	86	1	91	1.08e-37	121
MS.gene50668.t1	MTR_4g072570	63.333	90	29	1	1	86	1	90	1.55e-37	121
MS.gene50668.t1	MTR_4g072620	60.000	90	32	1	1	86	1	90	1.58e-37	121
MS.gene50668.t1	MTR_3g110042	65.169	89	27	1	1	85	1	89	1.95e-37	121
MS.gene50668.t1	MTR_4g072680	61.111	90	31	1	1	86	1	90	2.92e-37	120
MS.gene50668.t1	MTR_4g072730	60.000	90	32	1	1	86	1	90	1.91e-36	118
MS.gene50668.t1	MTR_4g072860	66.304	92	24	2	1	86	1	91	2.36e-36	118
MS.gene50668.t1	MTR_4g072500	63.333	90	29	1	1	86	1	90	2.56e-36	118
MS.gene50668.t1	MTR_3g117630	71.233	73	21	0	14	86	18	90	3.02e-36	117
MS.gene50668.t1	MTR_4g072230	64.835	91	25	2	1	86	1	89	4.17e-36	117
MS.gene50668.t1	MTR_3g084210	64.444	90	28	1	1	86	1	90	5.90e-36	117
MS.gene50668.t1	MTR_4g072460	58.889	90	33	1	1	86	1	90	6.80e-36	117
MS.gene50668.t1	MTR_8g461420	66.667	90	26	1	1	86	1	90	7.93e-36	117
MS.gene50668.t1	MTR_3g084190	65.556	90	27	1	1	86	1	90	9.98e-36	116
MS.gene50668.t1	MTR_4g072640	62.637	91	28	2	1	86	1	90	2.20e-35	115
MS.gene50668.t1	MTR_4g072530	61.111	90	31	1	1	86	1	90	3.51e-35	115
MS.gene50668.t1	MTR_3g084230	65.556	90	27	1	1	86	1	90	4.11e-35	115
MS.gene50668.t1	MTR_3g084160	64.444	90	28	1	1	86	1	90	1.34e-34	114
MS.gene50668.t1	MTR_4g072660	59.302	86	34	1	1	86	1	85	1.45e-34	113
MS.gene50668.t1	MTR_5g008360	64.103	78	28	0	9	86	4	81	3.46e-34	112
MS.gene50668.t1	MTR_3g110045	67.045	88	25	1	3	86	62	149	8.72e-34	113
MS.gene50668.t1	MTR_5g008370	62.222	90	30	1	1	86	1	90	4.51e-33	111
MS.gene50668.t1	MTR_4g072940	62.222	90	28	1	3	86	33	122	1.22e-32	109
MS.gene50668.t1	MTR_4g072560	52.577	97	34	3	1	85	1	97	2.03e-30	103
MS.gene50668.t1	MTR_4g072550	63.380	71	26	0	16	86	31	101	2.04e-29	101
MS.gene50668.t1	MTR_4g072980	52.174	92	37	2	1	85	1	92	2.11e-29	100
MS.gene50668.t1	MTR_3g084200	63.889	72	22	1	1	68	1	72	2.58e-29	100
MS.gene50668.t1	MTR_4g072770	56.977	86	31	1	1	86	1	80	4.70e-29	99.4
MS.gene50668.t1	MTR_4g072910	61.039	77	29	1	10	86	25	100	1.86e-28	98.6
MS.gene50668.t1	MTR_3g117640	58.824	68	27	1	20	86	33	100	1.12e-26	94.4
MS.gene50668.t1	MTR_3g110035	64.062	64	23	0	23	86	73	136	1.17e-25	92.8
MS.gene50668.t1	MTR_4g072290	55.814	86	29	2	1	86	1	77	1.75e-25	90.5
MS.gene50668.t1	MTR_4g072750	61.644	73	22	2	1	67	1	73	2.09e-23	85.5
MS.gene50668.t1	MTR_4g072280	52.000	75	31	1	1	70	1	75	6.79e-23	84.0
MS.gene50668.t1	MTR_3g113310	55.224	67	29	1	19	85	40	105	1.27e-22	84.0
MS.gene50668.t1	MTR_3g117610	58.462	65	26	1	19	83	43	106	3.26e-22	83.2
MS.gene50668.t1	MTR_6g077850	52.239	67	32	0	20	86	33	99	7.23e-22	82.0
MS.gene50668.t1	MTR_4g072190	49.333	75	37	1	11	85	26	99	2.09e-21	80.9
MS.gene50668.t1	MTR_4g072930	48.148	81	37	2	11	86	30	110	6.50e-21	79.7
MS.gene50668.t1	MTR_8g461400	46.667	90	18	2	1	86	1	64	3.00e-19	74.3
MS.gene50668.t1	MTR_4g072840	51.724	87	14	3	1	86	1	60	4.72e-19	73.6
MS.gene50668.t1	MTR_5g021820	45.238	84	43	2	4	85	21	103	8.32e-19	74.3
MS.gene50668.t1	MTR_8g076040	53.125	64	29	1	20	83	40	102	1.32e-17	71.2
MS.gene50668.t1	MTR_2g043960	52.542	59	27	1	20	78	75	132	7.45e-16	68.2
MS.gene50668.t1	MTR_4g124750	50.847	59	28	1	20	78	68	125	3.76e-15	66.2
MS.gene50668.t1	MTR_1g009150	46.377	69	36	1	13	81	63	130	5.67e-14	63.5
MS.gene50668.t1	MTR_1g094750	46.774	62	32	1	20	81	49	109	7.46e-14	62.0
MS.gene50668.t1	MTR_5g008380	84.375	32	5	0	55	86	246	277	1.47e-13	63.9
MS.gene50668.t1	MTR_4g005310	45.161	62	33	1	21	81	62	123	2.22e-13	61.6
MS.gene50668.t1	MTR_1g063950	45.161	62	33	1	20	81	47	107	3.12e-13	60.5
MS.gene50668.t1	MTR_1g061570	43.038	79	42	2	5	81	1	78	4.46e-13	60.1
MS.gene50668.t1	MTR_4g005320	45.902	61	32	1	21	81	70	129	7.10e-13	60.5
MS.gene50668.t1	MTR_3g109160	47.541	61	31	1	21	81	69	128	9.19e-13	60.5
MS.gene50668.t1	MTR_7g118010	41.176	68	37	2	21	86	23	89	1.45e-12	58.5
MS.gene50668.t1	MTR_7g101740	45.312	64	34	1	20	83	39	101	1.68e-12	58.5
MS.gene50668.t1	MTR_3g092220	36.364	77	46	2	12	86	24	99	5.95e-12	57.0
MS.gene50668.t1	MTR_3g105900	57.143	42	18	0	20	61	1171	1212	1.07e-11	59.3
MS.gene50668.t1	MTR_1g024085	44.068	59	32	1	20	78	36	93	1.58e-11	56.6
MS.gene50668.t1	MTR_4g072160	41.333	75	42	2	8	81	23	96	2.87e-11	55.8
MS.gene50668.t1	MTR_8g461210	37.975	79	44	2	4	78	14	91	6.88e-11	54.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50668.t1	AT4G38840	66.176	68	22	1	20	86	32	99	2.97e-31	105
MS.gene50668.t1	AT5G18080	63.514	74	26	1	14	86	17	90	3.56e-31	105
MS.gene50668.t1	AT5G18020	62.162	74	27	1	14	86	17	90	2.54e-30	103
MS.gene50668.t1	AT5G18050	61.538	78	26	2	10	86	16	90	3.51e-30	102
MS.gene50668.t1	AT5G18060	68.182	66	20	1	22	86	25	90	4.09e-30	102
MS.gene50668.t1	AT5G18030	60.759	79	27	2	9	86	13	88	5.62e-30	102
MS.gene50668.t1	AT5G18010	60.811	74	28	1	14	86	17	90	2.42e-29	100
MS.gene50668.t1	AT2G21210	53.086	81	36	1	8	86	15	95	5.30e-29	100
MS.gene50668.t1	AT4G38825	58.974	78	26	2	10	86	17	89	5.58e-29	99.8
MS.gene50668.t1	AT2G21200	61.111	72	27	1	15	85	15	86	7.29e-29	99.4
MS.gene50668.t1	AT4G34800	53.571	84	36	1	1	81	1	84	1.01e-28	99.0
MS.gene50668.t1	AT2G21210	53.086	81	36	1	8	86	82	162	3.07e-28	100
MS.gene50668.t1	AT4G38850	54.878	82	29	2	8	86	13	89	5.39e-28	97.1
MS.gene50668.t1	AT4G34810	60.494	81	27	2	10	85	37	117	7.52e-28	97.8
MS.gene50668.t1	AT3G03820	55.696	79	32	1	11	86	17	95	5.80e-27	94.7
MS.gene50668.t1	AT3G03840	57.692	78	31	1	11	86	17	94	6.09e-27	94.7
MS.gene50668.t1	AT4G13790	51.765	85	38	2	3	85	8	91	1.79e-26	93.2
MS.gene50668.t1	AT3G03850	61.194	67	24	1	22	86	26	92	6.00e-26	92.0
MS.gene50668.t1	AT3G03830	56.962	79	28	2	11	86	17	92	6.92e-25	89.4
MS.gene50668.t1	AT4G34770	53.571	84	37	1	5	86	19	102	2.75e-24	88.2
MS.gene50668.t1	AT4G38860	49.412	85	41	1	4	86	21	105	2.02e-23	85.9
MS.gene50668.t1	AT2G21220	47.619	84	41	2	4	85	21	103	1.73e-21	81.3
MS.gene50668.t1	AT4G34790	56.923	65	26	1	21	83	39	103	2.20e-21	80.9
MS.gene50668.t1	AT1G75580	55.224	67	29	1	19	85	42	107	5.68e-21	79.7
MS.gene50668.t1	AT4G34780	54.286	70	29	1	19	85	26	95	6.85e-21	79.7
MS.gene50668.t1	AT4G34760	53.731	67	30	1	19	85	41	106	5.71e-20	77.4
MS.gene50668.t1	AT2G16580	55.385	65	28	1	19	83	42	105	2.03e-19	75.9
MS.gene50668.t1	AT1G19830	55.072	69	29	2	19	86	46	113	7.63e-18	72.0
MS.gene50668.t1	AT4G36110	50.000	64	31	1	20	83	39	101	1.76e-17	70.9
MS.gene50668.t1	AT2G18010	51.562	64	30	1	20	83	47	109	3.26e-17	70.5
MS.gene50668.t1	AT3G43120	50.847	59	28	1	20	78	78	135	5.21e-16	68.6
MS.gene50668.t1	AT5G20810	52.542	59	27	1	20	78	78	135	2.35e-15	67.0
MS.gene50668.t1	AT5G20810	52.542	59	27	1	20	78	78	135	3.40e-15	67.0
MS.gene50668.t1	AT5G66260	45.455	66	35	1	21	86	34	98	9.32e-14	61.2
MS.gene50668.t1	AT4G31320	50.820	61	29	1	21	81	80	139	1.48e-13	62.8
MS.gene50668.t1	AT3G51200	41.463	82	42	3	5	81	19	99	6.31e-13	59.3
MS.gene50668.t1	AT4G34750	42.188	64	36	1	20	83	36	98	7.44e-13	60.1
MS.gene50668.t1	AT4G34750	42.188	64	36	1	20	83	36	98	7.44e-13	60.1
MS.gene50668.t1	AT2G24400	49.180	61	30	1	21	81	64	123	2.17e-12	59.7
MS.gene50668.t1	AT1G75590	44.068	59	32	1	20	78	41	98	2.98e-12	58.5
MS.gene50668.t1	AT3G60690	43.478	69	35	2	21	86	88	155	3.35e-12	58.9
MS.gene50668.t1	AT2G37030	44.444	63	34	1	20	82	48	109	4.60e-12	57.8
MS.gene50668.t1	AT2G45210	42.647	68	35	2	21	85	79	145	5.54e-12	58.2
MS.gene50668.t1	AT5G10990	40.000	75	39	2	4	78	29	97	9.32e-12	57.4
MS.gene50668.t1	AT1G19840	41.333	75	38	2	4	78	29	97	1.53e-11	57.0
MS.gene50668.t1	AT3G61900	35.443	79	46	3	12	86	20	97	2.24e-11	56.2
MS.gene50668.t1	AT2G46690	38.961	77	43	2	5	78	8	83	2.72e-11	55.5
MS.gene50668.t1	AT3G53250	41.270	63	33	1	20	82	51	109	5.58e-11	54.7

Find 21 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGGCTTGGCTCAACAATTCA+TGG	0.345764	4.3:+33339408	None:intergenic
GGCATCATCTTCGAAAGGTT+TGG	0.382114	4.3:-33339519	MS.gene50668:CDS
TACACTGCTAGATAGCCTTT+TGG	0.408055	4.3:+33339491	None:intergenic
GTCATATTCAAATTGTTCCT+CGG	0.462990	4.3:+33339388	None:intergenic
ATTCAAATTGTTCCTCGGCT+TGG	0.464907	4.3:+33339393	None:intergenic
GCTCAACAATTCATGGAATG+AGG	0.474793	4.3:+33339415	None:intergenic
TACCTAGTATCATCAGATCA+AGG	0.482621	4.3:-33339541	MS.gene50668:CDS
AAAGGTTTGGACTTGCCAAA+AGG	0.522930	4.3:-33339506	MS.gene50668:CDS
GCCCTTGATCTGATGATACT+AGG	0.523987	4.3:+33339539	None:intergenic
TTTGAATATGACCATCCAAT+GGG	0.542663	4.3:-33339377	MS.gene50668:CDS
ATTTGAATATGACCATCCAA+TGG	0.559485	4.3:-33339378	MS.gene50668:CDS
AAGAAAATGTCTTCTCTGCA+AGG	0.560690	4.3:+33339341	None:intergenic
ACCTAGTATCATCAGATCAA+GGG	0.588343	4.3:-33339540	MS.gene50668:CDS
TAGTTAGACCACCCATTGGA+TGG	0.598853	4.3:+33339366	None:intergenic
AGGCTATCTAGCAGTGTACG+TGG	0.607803	4.3:-33339486	MS.gene50668:CDS
GGAATAGTTAGACCACCCAT+TGG	0.610953	4.3:+33339362	None:intergenic
TCAAGGGCATCATCTTCGAA+AGG	0.614862	4.3:-33339524	MS.gene50668:CDS
GGCTATCTAGCAGTGTACGT+GGG	0.631886	4.3:-33339485	MS.gene50668:CDS
TGAATTGTTGAGCCAAGCCG+AGG	0.641874	4.3:-33339405	MS.gene50668:CDS
GAATATGACCATCCAATGGG+TGG	0.646056	4.3:-33339374	MS.gene50668:CDS
ACGTGGGAGAGAAAATGAAG+CGG	0.742198	4.3:-33339469	MS.gene50668:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTGTTCAAATATGATATA+GGG	+	chr4.3:33339435-33339454	None:intergenic	15.0%
!!!	GTTTTGTTCAAATATGATAT+AGG	+	chr4.3:33339436-33339455	None:intergenic	20.0%
	ATTTGAATATGACCATCCAA+TGG	-	chr4.3:33339492-33339511	MS.gene50668:CDS	30.0%
	GTCATATTCAAATTGTTCCT+CGG	+	chr4.3:33339485-33339504	None:intergenic	30.0%
	TTTGAATATGACCATCCAAT+GGG	-	chr4.3:33339493-33339512	MS.gene50668:CDS	30.0%
	AAGAAAATGTCTTCTCTGCA+AGG	+	chr4.3:33339532-33339551	None:intergenic	35.0%
	ACCTAGTATCATCAGATCAA+GGG	-	chr4.3:33339330-33339349	MS.gene50668:CDS	35.0%
	TACCTAGTATCATCAGATCA+AGG	-	chr4.3:33339329-33339348	MS.gene50668:CDS	35.0%
	AAAGGTTTGGACTTGCCAAA+AGG	-	chr4.3:33339364-33339383	MS.gene50668:CDS	40.0%
	ATTCAAATTGTTCCTCGGCT+TGG	+	chr4.3:33339480-33339499	None:intergenic	40.0%
	GCTCAACAATTCATGGAATG+AGG	+	chr4.3:33339458-33339477	None:intergenic	40.0%
!	TACACTGCTAGATAGCCTTT+TGG	+	chr4.3:33339382-33339401	None:intergenic	40.0%
	ACGTGGGAGAGAAAATGAAG+CGG	-	chr4.3:33339401-33339420	MS.gene50668:CDS	45.0%
	GAATATGACCATCCAATGGG+TGG	-	chr4.3:33339496-33339515	MS.gene50668:CDS	45.0%
	GCCCTTGATCTGATGATACT+AGG	+	chr4.3:33339334-33339353	None:intergenic	45.0%
	GGAATAGTTAGACCACCCAT+TGG	+	chr4.3:33339511-33339530	None:intergenic	45.0%
	GGCATCATCTTCGAAAGGTT+TGG	-	chr4.3:33339351-33339370	MS.gene50668:CDS	45.0%
	TAGTTAGACCACCCATTGGA+TGG	+	chr4.3:33339507-33339526	None:intergenic	45.0%
!!	TCAAGGGCATCATCTTCGAA+AGG	-	chr4.3:33339346-33339365	MS.gene50668:CDS	45.0%
	AGGCTATCTAGCAGTGTACG+TGG	-	chr4.3:33339384-33339403	MS.gene50668:CDS	50.0%
	GGCTATCTAGCAGTGTACGT+GGG	-	chr4.3:33339385-33339404	MS.gene50668:CDS	50.0%
	TGAATTGTTGAGCCAAGCCG+AGG	-	chr4.3:33339465-33339484	MS.gene50668:CDS	50.0%
!	CGGCTTGGCTCAACAATTCA+TGG	+	chr4.3:33339465-33339484	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	33339316	33339576	33339316	ID=MS.gene50668
chr4.3	mRNA	33339316	33339576	33339316	ID=MS.gene50668.t1;Parent=MS.gene50668
chr4.3	exon	33339316	33339576	33339316	ID=MS.gene50668.t1.exon1;Parent=MS.gene50668.t1
chr4.3	CDS	33339316	33339576	33339316	ID=cds.MS.gene50668.t1;Parent=MS.gene50668.t1

Gene Sequence	>MS.gene50668 ATGGGTTTTCGTTTACCTAGTATCATCAGATCAAGGGCATCATCTTCGAAAGGTTTGGACTTGCCAAAAGGCTATCTAGCAGTGTACGTGGGAGAGAAAATGAAGCGGTATCTGATCCCTATATCATATTTGAACAAAACCTCATTCCATGAATTGTTGAGCCAAGCCGAGGAACAATTTGAATATGACCATCCAATGGGTGGTCTAACTATTCCTTGCAGAGAAGACATTTTCTTAGATATCACTTCTCGTTTGAATTGA
Protein sequence	>MS.gene50668.t1 MGFRLPSIIRSRASSSKGLDLPKGYLAVYVGEKMKRYLIPISYLNKTSFHELLSQAEEQFEYDHPMGGLTIPCREDIFLDITSRLN