AlfalfaGEDB | MS.gene50674

M. sativa cultivar XinJiangDaYe / MS.gene50674

KEGG: K14488
GO: GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
PFAM: PF02519.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50674.t1	PNY03825.1	85.7	91	13	0	1	91	1	91	3.70E-38	167.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50674.t1	P33083	72.2	90	25	0	1	90	1	90	4.4e-32	138.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50674.t1	A0A2K3NLB0	85.7	91	13	0	1	91	1	91	2.7e-38	167.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene50674	MS.gene50684	PPI
MS.gene50674	MS.gene045366	PPI
MS.gene50674	MS.gene045362	PPI
MS.gene50674	MS.gene23372	PPI
MS.gene50674	MS.gene014668	PPI
MS.gene50763	MS.gene50674	PPI
MS.gene50674	MS.gene68164	PPI
MS.gene50674	MS.gene68759	PPI
MS.gene045365	MS.gene50674	PPI
MS.gene50674	MS.gene003586	PPI
MS.gene50674	MS.gene003627	PPI
MS.gene50659	MS.gene50674	PPI
MS.gene75316	MS.gene50674	PPI
MS.gene50674	MS.gene79891	PPI
MS.gene057790	MS.gene50674	PPI
MS.gene50674	MS.gene045364	PPI
MS.gene50674	MS.gene50687	PPI
MS.gene50674	MS.gene014223	PPI
MS.gene003624	MS.gene50674	PPI
MS.gene031201	MS.gene50674	PPI
MS.gene014667	MS.gene50674	PPI
MS.gene50674	MS.gene014669	PPI
MS.gene50674	MS.gene057808	PPI
MS.gene50674	MS.gene77526	PPI
MS.gene057778	MS.gene50674	PPI
MS.gene50674	MS.gene68163	PPI
MS.gene50674	MS.gene75315	PPI
MS.gene50674	MS.gene057818	PPI
MS.gene79890	MS.gene50674	PPI
MS.gene50674	MS.gene79889	PPI
MS.gene50674	MS.gene045368	PPI
MS.gene003615	MS.gene50674	PPI
MS.gene50674	MS.gene031200	PPI
MS.gene062973	MS.gene50674	PPI
MS.gene003602	MS.gene50674	PPI
MS.gene50674	MS.gene045360	PPI
MS.gene50674	MS.gene014222	PPI
MS.gene50674	MS.gene79888	PPI
MS.gene50674	MS.gene75676	PPI
MS.gene50674	MS.gene75678	PPI
MS.gene50674	MS.gene031199	PPI
MS.gene062984	MS.gene50674	PPI
MS.gene40327	MS.gene50674	PPI
MS.gene50674	MS.gene75673	PPI
MS.gene50674	MS.gene79885	PPI
MS.gene50674	MS.gene50764	PPI
MS.gene50674	MS.gene062963	PPI
MS.gene77523	MS.gene50674	PPI
MS.gene003587	MS.gene50674	PPI
MS.gene50674	MS.gene003577	PPI
MS.gene003590	MS.gene50674	PPI
MS.gene063112	MS.gene50674	PPI
MS.gene50674	MS.gene50675	PPI
MS.gene50674	MS.gene68162	PPI
MS.gene50674	MS.gene014670	PPI
MS.gene047517	MS.gene50674	PPI
MS.gene50674	MS.gene75314	PPI
MS.gene50674	MS.gene01764	PPI
MS.gene50674	MS.gene75679	PPI
MS.gene50674	MS.gene40325	PPI
MS.gene75677	MS.gene50674	PPI
MS.gene003625	MS.gene50674	PPI
MS.gene50674	MS.gene68760	PPI
MS.gene50674	MS.gene031198	PPI
MS.gene50674	MS.gene057797	PPI
MS.gene045361	MS.gene50674	PPI
MS.gene50674	MS.gene003614	PPI
MS.gene50674	MS.gene77528	PPI
MS.gene50674	MS.gene79886	PPI
MS.gene50674	MS.gene014671	PPI
MS.gene003591	MS.gene50674	PPI
MS.gene50674	MS.gene50765	PPI
MS.gene50674	MS.gene003596	PPI
MS.gene50674	MS.gene062988	PPI
MS.gene062979	MS.gene50674	PPI
MS.gene50674	MS.gene23371	PPI
MS.gene50674	MS.gene75672	PPI
MS.gene50674	MS.gene75674	PPI
MS.gene062943	MS.gene50674	PPI
MS.gene50674	MS.gene50654	PPI
MS.gene01358	MS.gene50674	PPI
MS.gene50674	MS.gene062974	PPI
MS.gene003608	MS.gene50674	PPI
MS.gene50674	MS.gene003583	PPI
MS.gene50674	MS.gene062951	PPI
MS.gene50674	MS.gene057801	PPI
MS.gene50674	MS.gene77527	PPI
MS.gene50674	MS.gene77524	PPI
MS.gene50674	MS.gene057795	PPI
MS.gene50674	MS.gene045367	PPI
MS.gene40326	MS.gene50674	PPI
MS.gene062952	MS.gene50674	PPI
MS.gene50674	MS.gene045363	PPI
MS.gene50674	MS.gene75675	PPI
MS.gene50674	MS.gene79887	PPI
MS.gene50676	MS.gene50674	PPI
MS.gene23370	MS.gene50674	PPI
MS.gene50674	MS.gene50652	PPI
MS.gene50766	MS.gene50674	PPI
MS.gene50674	MS.gene68761	PPI
MS.gene77529	MS.gene50674	PPI
MS.gene50674	MS.gene002380	PPI
MS.gene003594	MS.gene50674	PPI
MS.gene50665	MS.gene50674	PPI
MS.gene50674	MS.gene68165	PPI
MS.gene50674	MS.gene50677	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50674.t1	MTR_4g072620	81.319	91	17	0	1	91	1	91	6.26e-55	165
MS.gene50674.t1	MTR_4g072730	82.418	91	16	0	1	91	1	91	6.61e-55	165
MS.gene50674.t1	MTR_4g072480	81.319	91	17	0	1	91	1	91	8.14e-55	165
MS.gene50674.t1	MTR_4g072680	81.319	91	17	0	1	91	1	91	1.65e-53	161
MS.gene50674.t1	MTR_4g072460	79.121	91	19	0	1	91	1	91	5.29e-52	157
MS.gene50674.t1	MTR_4g072570	83.516	91	15	0	1	91	1	91	1.83e-51	156
MS.gene50674.t1	MTR_4g072500	79.121	91	19	0	1	91	1	91	8.89e-51	154
MS.gene50674.t1	MTR_4g072530	78.889	90	19	0	1	90	1	90	1.77e-49	151
MS.gene50674.t1	MTR_4g072640	77.778	90	20	0	1	90	1	90	5.05e-48	147
MS.gene50674.t1	MTR_4g072660	74.725	91	18	1	1	91	1	86	1.26e-47	146
MS.gene50674.t1	MTR_4g072270	71.739	92	25	1	1	91	1	92	5.45e-47	145
MS.gene50674.t1	MTR_3g084150	75.824	91	22	0	1	91	1	91	1.23e-46	144
MS.gene50674.t1	MTR_4g072310	72.826	92	24	1	1	91	1	92	2.30e-46	144
MS.gene50674.t1	MTR_3g084180	74.725	91	23	0	1	91	1	91	1.33e-45	142
MS.gene50674.t1	MTR_8g461410	73.626	91	24	0	1	91	1	91	3.03e-45	140
MS.gene50674.t1	MTR_4g072860	72.826	92	24	1	1	91	1	92	6.17e-45	140
MS.gene50674.t1	MTR_4g072880	70.652	92	26	1	1	91	1	92	2.13e-44	139
MS.gene50674.t1	MTR_4g072230	72.826	92	22	2	1	91	1	90	2.32e-43	136
MS.gene50674.t1	MTR_4g072250	72.826	92	23	2	1	91	1	91	2.98e-43	135
MS.gene50674.t1	MTR_3g084240	69.231	91	28	0	1	91	1	91	1.17e-42	134
MS.gene50674.t1	MTR_5g008360	79.747	79	16	0	13	91	4	82	1.98e-42	133
MS.gene50674.t1	MTR_4g072240	67.033	91	29	1	1	90	1	91	2.20e-42	133
MS.gene50674.t1	MTR_3g110042	74.713	87	22	0	1	87	1	87	2.27e-42	134
MS.gene50674.t1	MTR_3g084170	69.231	91	28	0	1	91	1	91	2.37e-42	133
MS.gene50674.t1	MTR_5g008370	70.330	91	27	0	1	91	1	91	3.40e-42	134
MS.gene50674.t1	MTR_4g072870	68.132	91	28	1	1	90	1	91	5.01e-42	132
MS.gene50674.t1	MTR_4g050700	69.565	92	27	1	1	91	1	92	1.31e-41	131
MS.gene50674.t1	MTR_2g012410	69.565	92	27	1	1	91	1	92	1.31e-41	131
MS.gene50674.t1	MTR_3g117630	75.000	84	21	0	7	90	7	90	2.37e-41	131
MS.gene50674.t1	MTR_4g072300	64.835	91	31	1	1	90	1	91	2.99e-41	130
MS.gene50674.t1	MTR_3g110032	71.739	92	25	1	1	91	1	92	4.41e-41	130
MS.gene50674.t1	MTR_3g084220	67.033	91	30	0	1	91	1	91	4.83e-41	130
MS.gene50674.t1	MTR_4g072220	64.835	91	31	1	1	90	1	91	1.66e-40	129
MS.gene50674.t1	MTR_4g072330	68.889	90	26	1	1	90	1	88	3.43e-40	128
MS.gene50674.t1	MTR_4g072370	70.000	90	25	1	1	90	1	88	4.93e-40	127
MS.gene50674.t1	MTR_4g072850	69.565	92	26	1	1	90	1	92	7.45e-40	127
MS.gene50674.t1	MTR_3g084250	67.033	91	30	0	1	91	1	91	2.10e-39	126
MS.gene50674.t1	MTR_4g072580	68.889	90	23	1	1	90	1	85	3.96e-39	125
MS.gene50674.t1	MTR_3g084260	68.478	92	28	1	1	91	1	92	4.99e-39	125
MS.gene50674.t1	MTR_4g072600	70.000	90	22	1	1	90	1	85	5.56e-39	125
MS.gene50674.t1	MTR_4g072490	66.667	90	25	1	1	90	1	85	7.70e-39	124
MS.gene50674.t1	MTR_4g072830	69.663	89	25	1	1	89	1	87	8.62e-39	124
MS.gene50674.t1	MTR_4g072630	67.778	90	25	1	1	90	1	86	1.26e-38	124
MS.gene50674.t1	MTR_3g110038	64.444	90	32	0	1	90	1	90	2.96e-38	123
MS.gene50674.t1	MTR_4g072470	68.889	90	23	1	1	90	1	85	3.58e-38	122
MS.gene50674.t1	MTR_4g072550	75.000	72	18	0	20	91	31	102	3.63e-38	123
MS.gene50674.t1	MTR_4g072890	61.538	91	34	1	1	90	1	91	4.75e-38	122
MS.gene50674.t1	MTR_4g072740	66.667	90	25	1	1	90	1	85	6.98e-38	122
MS.gene50674.t1	MTR_4g072260	61.538	91	34	1	1	90	1	91	9.11e-38	122
MS.gene50674.t1	MTR_3g084210	65.934	91	31	0	1	91	1	91	1.45e-37	121
MS.gene50674.t1	MTR_4g072465	66.667	90	26	1	1	90	1	86	3.77e-37	120
MS.gene50674.t1	MTR_8g461420	66.667	90	30	0	1	90	1	90	1.64e-36	119
MS.gene50674.t1	MTR_3g084190	65.556	90	31	0	1	90	1	90	1.89e-36	118
MS.gene50674.t1	MTR_4g072940	67.778	90	27	1	3	90	33	122	2.20e-36	119
MS.gene50674.t1	MTR_3g084230	66.667	90	30	0	1	90	1	90	5.52e-36	117
MS.gene50674.t1	MTR_4g072610	66.667	90	24	1	1	90	1	84	7.36e-36	117
MS.gene50674.t1	MTR_4g072540	67.778	90	25	1	1	90	1	86	1.26e-35	116
MS.gene50674.t1	MTR_3g084160	64.444	90	32	0	1	90	1	90	2.51e-35	115
MS.gene50674.t1	MTR_4g072820	67.778	90	22	2	1	89	1	84	6.59e-35	114
MS.gene50674.t1	MTR_4g072590	68.889	90	23	1	1	90	1	85	6.69e-35	114
MS.gene50674.t1	MTR_4g072720	66.667	90	26	1	1	90	1	86	1.87e-34	113
MS.gene50674.t1	MTR_4g072670	67.778	90	25	1	1	90	1	86	2.33e-34	113
MS.gene50674.t1	MTR_4g072380	63.736	91	27	2	1	90	1	86	4.93e-34	112
MS.gene50674.t1	MTR_4g072650	64.444	90	26	1	1	90	1	84	6.59e-34	112
MS.gene50674.t1	MTR_3g110045	67.045	88	29	0	3	90	62	149	6.79e-34	114
MS.gene50674.t1	MTR_4g072510	64.444	90	28	1	1	90	1	86	7.77e-33	109
MS.gene50674.t1	MTR_5g008350	64.557	79	23	1	1	79	1	74	3.26e-32	107
MS.gene50674.t1	MTR_4g072690	67.778	90	24	1	1	90	1	85	6.26e-32	107
MS.gene50674.t1	MTR_4g072750	72.603	73	18	1	1	71	1	73	1.98e-31	105
MS.gene50674.t1	MTR_4g072450	67.778	90	24	1	1	90	1	85	2.16e-31	105
MS.gene50674.t1	MTR_4g072290	58.696	92	23	2	1	91	1	78	3.22e-30	102
MS.gene50674.t1	MTR_4g072910	58.621	87	33	2	4	90	17	100	3.51e-30	103
MS.gene50674.t1	MTR_4g072980	54.444	90	35	2	1	89	8	92	6.30e-30	102
MS.gene50674.t1	MTR_4g072770	71.212	66	19	0	25	90	15	80	1.15e-29	101
MS.gene50674.t1	MTR_3g117640	69.565	69	20	1	24	91	33	101	1.49e-29	101
MS.gene50674.t1	MTR_4g072560	51.546	97	39	2	1	89	1	97	2.63e-29	100
MS.gene50674.t1	MTR_3g084200	63.889	72	26	0	1	72	1	72	3.94e-29	100
MS.gene50674.t1	MTR_3g110035	63.077	65	24	0	27	91	73	137	6.34e-26	93.6
MS.gene50674.t1	MTR_4g072280	58.667	75	30	1	1	74	1	75	3.51e-25	90.1
MS.gene50674.t1	MTR_6g077850	53.731	67	31	0	24	90	33	99	2.69e-24	88.2
MS.gene50674.t1	MTR_3g117610	56.716	67	28	1	23	89	43	108	2.23e-23	86.3
MS.gene50674.t1	MTR_4g072190	49.412	85	41	2	5	89	17	99	2.47e-23	85.9
MS.gene50674.t1	MTR_3g113310	53.731	67	30	1	23	89	40	105	2.98e-23	85.9
MS.gene50674.t1	MTR_8g076040	54.545	66	29	1	24	89	40	104	4.39e-21	80.1
MS.gene50674.t1	MTR_5g021820	54.545	66	29	1	24	89	39	103	1.15e-20	79.3
MS.gene50674.t1	MTR_4g072930	49.383	81	36	2	15	90	30	110	1.36e-20	79.3
MS.gene50674.t1	MTR_4g124750	43.182	88	47	2	4	89	46	132	4.92e-19	76.3
MS.gene50674.t1	MTR_2g043960	47.436	78	40	1	15	92	66	142	5.35e-19	76.6
MS.gene50674.t1	MTR_8g461400	43.956	91	25	1	1	91	1	65	9.10e-19	73.2
MS.gene50674.t1	MTR_8g461210	44.186	86	47	1	4	89	14	98	5.65e-17	70.1
MS.gene50674.t1	MTR_4g072840	45.556	90	17	3	1	89	1	59	6.31e-17	68.6
MS.gene50674.t1	MTR_1g094750	43.529	85	43	3	5	85	26	109	9.67e-16	67.0
MS.gene50674.t1	MTR_7g118010	47.059	68	33	2	25	90	23	89	1.18e-15	66.6
MS.gene50674.t1	MTR_3g092220	40.000	85	45	3	1	83	12	92	1.29e-15	66.6
MS.gene50674.t1	MTR_1g024085	45.783	83	41	2	4	83	13	94	2.37e-15	66.6
MS.gene50674.t1	MTR_8g026730	45.588	68	34	2	25	90	27	93	5.69e-15	64.7
MS.gene50674.t1	MTR_4g005310	48.485	66	33	1	21	85	58	123	5.70e-15	65.9
MS.gene50674.t1	MTR_1g009150	50.000	68	33	1	18	85	64	130	1.25e-14	65.5
MS.gene50674.t1	MTR_0388s0020	38.824	85	48	2	8	89	58	141	4.03e-14	63.5
MS.gene50674.t1	MTR_4g072160	44.444	81	39	3	6	85	21	96	6.75e-14	62.8
MS.gene50674.t1	MTR_1g059770	42.647	68	35	2	28	92	52	118	1.71e-13	61.6
MS.gene50674.t1	MTR_3g109160	40.741	81	47	1	12	92	56	135	2.24e-13	62.0
MS.gene50674.t1	MTR_4g005320	44.118	68	37	1	25	92	70	136	2.25e-13	62.0
MS.gene50674.t1	MTR_1g061570	45.588	68	36	1	24	91	18	84	2.38e-13	61.2
MS.gene50674.t1	MTR_7g104940	38.667	75	41	2	15	89	44	113	2.65e-13	61.2
MS.gene50674.t1	MTR_1g063950	40.278	72	42	1	17	88	40	110	3.67e-13	60.5
MS.gene50674.t1	MTR_7g104950	35.526	76	44	2	14	89	43	113	3.75e-13	60.8
MS.gene50674.t1	MTR_1g063310	42.105	76	41	2	17	90	18	92	6.22e-13	59.3
MS.gene50674.t1	MTR_8g101520	39.773	88	48	2	7	90	28	114	8.67e-13	60.5
MS.gene50674.t1	MTR_4g025830	43.939	66	33	2	25	87	79	143	8.68e-13	60.5
MS.gene50674.t1	MTR_3g097120	43.243	74	33	2	20	85	4	76	1.79e-12	58.5
MS.gene50674.t1	MTR_7g101740	45.312	64	34	1	24	87	39	101	2.17e-12	58.5
MS.gene50674.t1	MTR_2g066830	37.037	81	50	1	10	90	43	122	3.23e-12	58.5
MS.gene50674.t1	MTR_8g101530	37.333	75	46	1	13	87	28	101	5.54e-12	58.2
MS.gene50674.t1	MTR_2g095430	38.750	80	41	3	5	77	22	100	6.68e-12	57.4
MS.gene50674.t1	MTR_3g105900	72.222	36	10	0	24	59	1171	1206	1.56e-11	58.9
MS.gene50674.t1	MTR_1g063290	42.188	64	34	2	24	85	38	100	2.06e-11	55.8
MS.gene50674.t1	MTR_7g101890	37.975	79	46	2	9	85	36	113	2.55e-11	55.8
MS.gene50674.t1	MTR_1g063300	42.188	64	34	2	24	85	38	100	3.39e-11	55.1
MS.gene50674.t1	MTR_4g021810	50.000	62	29	2	25	85	26	86	3.94e-11	55.1
MS.gene50674.t1	MTR_8g096470	44.828	58	31	1	28	85	49	105	4.44e-11	55.1
MS.gene50674.t1	MTR_1g063330	40.909	66	36	2	24	87	31	95	5.08e-11	54.3
MS.gene50674.t1	MTR_3g098970	32.468	77	51	1	15	91	43	118	8.20e-11	55.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50674.t1	AT4G34770	62.222	90	32	1	4	91	14	103	2.35e-34	114
MS.gene50674.t1	AT4G34800	60.000	85	27	2	1	82	1	81	3.60e-31	105
MS.gene50674.t1	AT5G18060	53.846	91	40	2	1	90	1	90	7.80e-31	104
MS.gene50674.t1	AT5G18020	54.545	88	38	2	1	87	1	87	1.34e-30	103
MS.gene50674.t1	AT4G38840	54.545	99	36	3	1	90	1	99	2.17e-30	103
MS.gene50674.t1	AT5G18080	53.333	90	40	2	1	89	1	89	5.99e-30	102
MS.gene50674.t1	AT2G21200	55.952	84	35	2	8	89	3	86	6.73e-30	102
MS.gene50674.t1	AT2G21210	50.526	95	42	2	1	90	1	95	1.13e-29	102
MS.gene50674.t1	AT4G38825	61.333	75	28	1	17	90	15	89	1.17e-29	101
MS.gene50674.t1	AT5G18050	68.750	64	19	1	27	89	26	89	1.21e-29	101
MS.gene50674.t1	AT5G18030	54.118	85	36	2	8	89	3	87	4.02e-29	100
MS.gene50674.t1	AT2G21210	50.526	95	42	2	1	90	68	162	5.15e-29	102
MS.gene50674.t1	AT5G18010	51.111	90	42	2	1	89	1	89	6.15e-28	97.4
MS.gene50674.t1	AT4G38850	55.556	90	33	3	1	87	1	86	3.38e-27	95.5
MS.gene50674.t1	AT3G03840	58.904	73	28	1	21	91	23	95	4.76e-27	95.1
MS.gene50674.t1	AT4G34810	69.355	62	15	1	25	82	48	109	5.12e-27	95.9
MS.gene50674.t1	AT3G03820	51.685	89	37	2	6	91	11	96	1.17e-26	94.4
MS.gene50674.t1	AT3G03850	65.672	67	21	1	27	91	27	93	1.43e-26	94.0
MS.gene50674.t1	AT4G13790	44.944	89	47	1	1	87	1	89	3.12e-25	90.5
MS.gene50674.t1	AT3G03830	57.746	71	27	1	20	87	19	89	8.64e-25	89.4
MS.gene50674.t1	AT4G34780	61.429	70	24	1	23	89	26	95	1.27e-24	89.4
MS.gene50674.t1	AT4G34790	61.538	65	23	1	25	87	39	103	2.73e-23	85.9
MS.gene50674.t1	AT1G75580	50.000	80	39	1	10	89	29	107	1.73e-22	84.0
MS.gene50674.t1	AT4G34760	55.224	67	29	1	23	89	41	106	2.55e-22	83.6
MS.gene50674.t1	AT4G38860	52.941	68	32	0	23	90	38	105	6.52e-22	82.4
MS.gene50674.t1	AT2G21220	53.731	67	30	1	23	89	38	103	9.20e-22	82.0
MS.gene50674.t1	AT1G19830	55.405	74	31	2	17	89	40	112	2.09e-20	79.0
MS.gene50674.t1	AT3G43120	41.111	90	52	1	3	92	57	145	4.24e-20	79.3
MS.gene50674.t1	AT2G16580	53.846	65	29	1	23	87	42	105	9.05e-20	77.0
MS.gene50674.t1	AT5G20810	51.515	66	31	1	24	89	78	142	3.38e-18	74.7
MS.gene50674.t1	AT4G36110	51.562	64	30	1	24	87	39	101	4.12e-18	72.8
MS.gene50674.t1	AT5G20810	47.945	73	37	1	17	89	71	142	4.60e-18	74.7
MS.gene50674.t1	AT2G18010	51.562	64	30	1	24	87	47	109	6.55e-18	72.4
MS.gene50674.t1	AT3G20220	42.697	89	43	3	4	85	20	107	2.90e-17	70.9
MS.gene50674.t1	AT2G46690	38.889	90	51	2	6	92	5	93	4.76e-17	70.5
MS.gene50674.t1	AT5G66260	43.820	89	46	2	5	90	11	98	1.08e-16	68.9
MS.gene50674.t1	AT3G12830	42.529	87	48	2	4	90	30	114	2.61e-16	68.9
MS.gene50674.t1	AT3G51200	50.877	57	27	1	26	82	41	96	3.60e-15	65.5
MS.gene50674.t1	AT2G37030	47.692	65	33	1	24	88	48	111	1.62e-14	63.9
MS.gene50674.t1	AT3G61900	37.234	94	49	3	6	90	5	97	2.14e-14	63.9
MS.gene50674.t1	AT1G75590	42.353	85	43	2	4	83	16	99	2.33e-14	64.3
MS.gene50674.t1	AT4G34750	43.750	64	35	1	24	87	36	98	8.47e-14	62.8
MS.gene50674.t1	AT4G34750	43.750	64	35	1	24	87	36	98	8.47e-14	62.8
MS.gene50674.t1	AT4G00880	47.826	69	33	2	24	90	27	94	8.65e-14	62.4
MS.gene50674.t1	AT5G10990	42.857	84	43	3	4	83	16	98	1.21e-13	62.4
MS.gene50674.t1	AT1G19840	44.048	84	42	2	4	83	16	98	2.07e-13	62.0
MS.gene50674.t1	AT3G09870	48.571	70	33	2	18	85	38	106	6.20e-13	59.7
MS.gene50674.t1	AT5G53590	40.278	72	37	2	24	90	45	115	7.32e-13	60.5
MS.gene50674.t1	AT4G31320	43.939	66	36	1	20	85	75	139	1.32e-12	60.5
MS.gene50674.t1	AT1G56150	45.455	66	35	1	25	90	38	102	2.26e-12	58.2
MS.gene50674.t1	AT2G45210	39.437	71	39	2	20	87	74	143	2.66e-12	59.3
MS.gene50674.t1	AT2G24400	49.180	61	30	1	25	85	64	123	6.80e-12	58.5
MS.gene50674.t1	AT1G16510	44.615	65	35	1	25	89	54	117	7.62e-12	57.8
MS.gene50674.t1	AT3G53250	40.000	65	35	1	24	88	51	111	8.01e-12	57.4
MS.gene50674.t1	AT3G60690	41.429	70	37	2	21	87	84	152	1.08e-11	57.8
MS.gene50674.t1	AT1G76190	48.333	60	29	2	26	85	22	79	1.25e-11	56.6
MS.gene50674.t1	AT1G79130	43.077	65	36	1	25	89	48	111	1.59e-11	56.6
MS.gene50674.t1	AT3G03847	39.024	82	46	4	3	82	48	127	2.65e-11	55.8
MS.gene50674.t1	AT4G09530	37.662	77	45	2	19	92	22	98	2.92e-11	55.1
MS.gene50674.t1	AT4G12410	42.188	64	33	2	25	85	76	138	4.78e-11	55.8

Find 30 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCCGTTTACAAACTATTCTA+AGG	0.281110	4.3:-33493134	MS.gene50674:CDS
CACATAACTTCTTGCTTGAA+TGG	0.304861	4.3:-33492892	MS.gene50674:CDS
TGGCTGGTTCAAGTATGATA+TGG	0.304862	4.3:+33493011	None:intergenic
AGTCAAGCCGAACAAGAGTT+TGG	0.385753	4.3:-33492970	MS.gene50674:CDS
TTGAACCAGCCATCGTTTCA+AGG	0.412783	4.3:-33493000	MS.gene50674:CDS
CCCTTAGAATAGTTTGTAAA+CGG	0.415563	4.3:+33493133	None:intergenic
ATCATATCCAAACTCTTGTT+CGG	0.425277	4.3:+33492963	None:intergenic
GGCTGGTTCAAGTATGATAT+GGG	0.431694	4.3:+33493012	None:intergenic
TATGTGTTGGAAGGCTTCTT+CGG	0.441042	4.3:+33492909	None:intergenic
ACATAACTTCTTGCTTGAAT+GGG	0.448638	4.3:-33492891	MS.gene50674:CDS
AAAATCTGTAGAAGTGAGAA+AGG	0.471464	4.3:-33493082	MS.gene50674:CDS
AAATCTGTAGAAGTGAGAAA+GGG	0.501254	4.3:-33493081	MS.gene50674:CDS
ATGTAGGAGAGGAGCAGAAG+AGG	0.513132	4.3:-33493044	MS.gene50674:CDS
GGCTTCTTCGGTGCAAGGAA+TGG	0.529253	4.3:+33492921	None:intergenic
CCGTTTACAAACTATTCTAA+GGG	0.531520	4.3:-33493133	MS.gene50674:CDS
GGGTATGTTGCAGTGTATGT+AGG	0.540211	4.3:-33493060	MS.gene50674:CDS
TGGAAGGCTTCTTCGGTGCA+AGG	0.543329	4.3:+33492916	None:intergenic
AGGAATGGTGAGGCCACCCA+TGG	0.554354	4.3:+33492936	None:intergenic
TCAAGCAAGAAGTTATGTGT+TGG	0.558158	4.3:+33492896	None:intergenic
CTCAGTAAGCCTTGAAACGA+TGG	0.561145	4.3:+33492991	None:intergenic
GTTTGGATATGATCATCCCA+TGG	0.570301	4.3:-33492953	MS.gene50674:CDS
GCAAGAAGTTATGTGTTGGA+AGG	0.573373	4.3:+33492900	None:intergenic
GTAAGCCTTGAAACGATGGC+TGG	0.590778	4.3:+33492995	None:intergenic
GGATATGATCATCCCATGGG+TGG	0.600402	4.3:-33492949	MS.gene50674:CDS
TGTTGCAGTGTATGTAGGAG+AGG	0.621670	4.3:-33493055	MS.gene50674:CDS
GGAATGGTGAGGCCACCCAT+GGG	0.636587	4.3:+33492937	None:intergenic
TTTGGATATGATCATCCCAT+GGG	0.641263	4.3:-33492952	MS.gene50674:CDS
AATCTGTAGAAGTGAGAAAG+GGG	0.642438	4.3:-33493080	MS.gene50674:CDS
CTTCGGTGCAAGGAATGGTG+AGG	0.665194	4.3:+33492926	None:intergenic
GCTGGTTCAAGTATGATATG+GGG	0.687425	4.3:+33493013	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAAATCTGTAGAAGTGAGAA+AGG	-	chr4.3:33492944-33492963	MS.gene50674:CDS	30.0%
	AAATCTGTAGAAGTGAGAAA+GGG	-	chr4.3:33492945-33492964	MS.gene50674:CDS	30.0%
	ACATAACTTCTTGCTTGAAT+GGG	-	chr4.3:33493135-33493154	MS.gene50674:CDS	30.0%
	ATCATATCCAAACTCTTGTT+CGG	+	chr4.3:33493066-33493085	None:intergenic	30.0%
	CCGTTTACAAACTATTCTAA+GGG	-	chr4.3:33492893-33492912	MS.gene50674:CDS	30.0%
	TCCGTTTACAAACTATTCTA+AGG	-	chr4.3:33492892-33492911	MS.gene50674:CDS	30.0%
!	CCCTTAGAATAGTTTGTAAA+CGG	+	chr4.3:33492896-33492915	None:intergenic	30.0%
	AATCTGTAGAAGTGAGAAAG+GGG	-	chr4.3:33492946-33492965	MS.gene50674:CDS	35.0%
	CACATAACTTCTTGCTTGAA+TGG	-	chr4.3:33493134-33493153	MS.gene50674:CDS	35.0%
	TCAAGCAAGAAGTTATGTGT+TGG	+	chr4.3:33493133-33493152	None:intergenic	35.0%
	TTTGGATATGATCATCCCAT+GGG	-	chr4.3:33493074-33493093	MS.gene50674:CDS	35.0%
	GCAAGAAGTTATGTGTTGGA+AGG	+	chr4.3:33493129-33493148	None:intergenic	40.0%
	GTTTGGATATGATCATCCCA+TGG	-	chr4.3:33493073-33493092	MS.gene50674:CDS	40.0%
	TATGTGTTGGAAGGCTTCTT+CGG	+	chr4.3:33493120-33493139	None:intergenic	40.0%
!	GCTGGTTCAAGTATGATATG+GGG	+	chr4.3:33493016-33493035	None:intergenic	40.0%
!	GGCTGGTTCAAGTATGATAT+GGG	+	chr4.3:33493017-33493036	None:intergenic	40.0%
!	TGGCTGGTTCAAGTATGATA+TGG	+	chr4.3:33493018-33493037	None:intergenic	40.0%
	AGTCAAGCCGAACAAGAGTT+TGG	-	chr4.3:33493056-33493075	MS.gene50674:CDS	45.0%
	CTCAGTAAGCCTTGAAACGA+TGG	+	chr4.3:33493038-33493057	None:intergenic	45.0%
	GGGTATGTTGCAGTGTATGT+AGG	-	chr4.3:33492966-33492985	MS.gene50674:CDS	45.0%
	TGTTGCAGTGTATGTAGGAG+AGG	-	chr4.3:33492971-33492990	MS.gene50674:CDS	45.0%
	TTGAACCAGCCATCGTTTCA+AGG	-	chr4.3:33493026-33493045	MS.gene50674:CDS	45.0%
	ATGTAGGAGAGGAGCAGAAG+AGG	-	chr4.3:33492982-33493001	MS.gene50674:CDS	50.0%
	GGATATGATCATCCCATGGG+TGG	-	chr4.3:33493077-33493096	MS.gene50674:CDS	50.0%
	GTAAGCCTTGAAACGATGGC+TGG	+	chr4.3:33493034-33493053	None:intergenic	50.0%
!	CTTCGGTGCAAGGAATGGTG+AGG	+	chr4.3:33493103-33493122	None:intergenic	55.0%
!	GGCTTCTTCGGTGCAAGGAA+TGG	+	chr4.3:33493108-33493127	None:intergenic	55.0%
!	TGGAAGGCTTCTTCGGTGCA+AGG	+	chr4.3:33493113-33493132	None:intergenic	55.0%
	AGGAATGGTGAGGCCACCCA+TGG	+	chr4.3:33493093-33493112	None:intergenic	60.0%
	GGAATGGTGAGGCCACCCAT+GGG	+	chr4.3:33493092-33493111	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	33492885	33493163	33492885	ID=MS.gene50674
chr4.3	mRNA	33492885	33493163	33492885	ID=MS.gene50674.t1;Parent=MS.gene50674
chr4.3	exon	33492885	33493163	33492885	ID=MS.gene50674.t1.exon1;Parent=MS.gene50674.t1
chr4.3	CDS	33492885	33493163	33492885	ID=cds.MS.gene50674.t1;Parent=MS.gene50674.t1

Gene Sequence	>MS.gene50674 ATGGGTTTCCGTTTACAAACTATTCTAAGGGCATCAGTTACTTCTAGACAAGCATCATCAAAATCTGTAGAAGTGAGAAAGGGGTATGTTGCAGTGTATGTAGGAGAGGAGCAGAAGAGGTTTGTGATCCCCATATCATACTTGAACCAGCCATCGTTTCAAGGCTTACTGAGTCAAGCCGAACAAGAGTTTGGATATGATCATCCCATGGGTGGCCTCACCATTCCTTGCACCGAAGAAGCCTTCCAACACATAACTTCTTGCTTGAATGGGCAATAA
Protein sequence	>MS.gene50674.t1 MGFRLQTILRASVTSRQASSKSVEVRKGYVAVYVGEEQKRFVIPISYLNQPSFQGLLSQAEQEFGYDHPMGGLTIPCTEEAFQHITSCLNGQ