AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50683

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50683.t1 MTR_4g072220 96.774 93 3 0 1 93 1 93 5.16e-63 186
MS.gene50683.t1 MTR_4g072240 87.912 91 11 0 1 91 1 91 1.78e-57 171
MS.gene50683.t1 MTR_4g072300 86.813 91 12 0 1 91 1 91 5.16e-57 170
MS.gene50683.t1 MTR_4g072890 89.011 91 10 0 1 91 1 91 9.14e-57 170
MS.gene50683.t1 MTR_4g072260 87.097 93 12 0 1 93 1 93 1.25e-56 169
MS.gene50683.t1 MTR_4g072870 82.418 91 16 0 1 91 1 91 4.95e-52 158
MS.gene50683.t1 MTR_4g072310 67.742 93 30 0 1 93 1 93 1.18e-44 139
MS.gene50683.t1 MTR_4g072500 69.892 93 27 1 1 93 1 92 1.87e-44 139
MS.gene50683.t1 MTR_4g072640 69.565 92 27 1 1 92 1 91 1.07e-43 137
MS.gene50683.t1 MTR_4g072530 68.132 91 28 1 1 91 1 90 1.67e-42 134
MS.gene50683.t1 MTR_4g072880 65.591 93 32 0 1 93 1 93 3.38e-42 133
MS.gene50683.t1 MTR_4g072250 68.132 91 28 1 1 91 1 90 3.54e-42 133
MS.gene50683.t1 MTR_4g072230 66.667 93 29 1 1 93 1 91 4.28e-42 133
MS.gene50683.t1 MTR_4g072570 66.667 93 30 1 1 93 1 92 2.03e-41 131
MS.gene50683.t1 MTR_4g072280 82.667 75 13 0 1 75 1 75 2.69e-41 130
MS.gene50683.t1 MTR_3g084150 67.742 93 29 1 1 93 1 92 3.07e-41 130
MS.gene50683.t1 MTR_3g110038 69.892 93 27 1 1 93 1 92 3.07e-41 130
MS.gene50683.t1 MTR_4g072370 68.132 91 26 1 1 91 1 88 3.61e-41 130
MS.gene50683.t1 MTR_3g084240 67.742 93 29 1 1 93 1 92 9.41e-41 129
MS.gene50683.t1 MTR_3g084180 66.667 93 30 1 1 93 1 92 1.88e-40 129
MS.gene50683.t1 MTR_4g072480 65.591 93 31 1 1 93 1 92 1.90e-40 129
MS.gene50683.t1 MTR_3g117630 66.667 90 30 0 4 93 3 92 2.55e-40 128
MS.gene50683.t1 MTR_3g084170 67.742 93 29 1 1 93 1 92 4.00e-40 128
MS.gene50683.t1 MTR_4g072860 64.516 93 33 0 1 93 1 93 5.30e-40 127
MS.gene50683.t1 MTR_4g072850 69.149 94 28 1 1 93 1 94 5.46e-40 127
MS.gene50683.t1 MTR_4g072580 68.132 91 23 1 1 91 1 85 5.66e-40 127
MS.gene50683.t1 MTR_4g072330 67.033 91 27 1 1 91 1 88 7.69e-40 127
MS.gene50683.t1 MTR_8g461410 65.591 93 31 1 1 93 1 92 8.43e-40 127
MS.gene50683.t1 MTR_4g072270 63.441 93 34 0 1 93 1 93 9.69e-40 127
MS.gene50683.t1 MTR_4g072730 63.736 91 32 1 1 91 1 90 9.94e-40 127
MS.gene50683.t1 MTR_4g072830 68.132 91 26 1 1 91 1 88 1.85e-39 126
MS.gene50683.t1 MTR_4g050700 65.591 93 32 0 1 93 1 93 2.16e-39 126
MS.gene50683.t1 MTR_2g012410 65.591 93 32 0 1 93 1 93 2.16e-39 126
MS.gene50683.t1 MTR_3g110032 64.516 93 33 0 1 93 1 93 3.20e-39 125
MS.gene50683.t1 MTR_4g072620 62.366 93 34 1 1 93 1 92 3.87e-39 125
MS.gene50683.t1 MTR_3g084250 67.742 93 29 1 1 93 1 92 9.01e-39 124
MS.gene50683.t1 MTR_4g072680 62.366 93 34 1 1 93 1 92 1.29e-38 124
MS.gene50683.t1 MTR_4g072740 67.033 91 24 1 1 91 1 85 1.73e-38 123
MS.gene50683.t1 MTR_4g072600 67.033 91 24 1 1 91 1 85 2.63e-38 123
MS.gene50683.t1 MTR_4g072630 65.934 91 26 1 1 91 1 86 3.92e-38 122
MS.gene50683.t1 MTR_4g072460 61.290 93 35 1 1 93 1 92 1.72e-37 121
MS.gene50683.t1 MTR_4g072490 64.835 91 26 1 1 91 1 85 2.15e-37 120
MS.gene50683.t1 MTR_3g084220 64.516 93 32 1 1 93 1 92 2.17e-37 121
MS.gene50683.t1 MTR_3g110042 65.556 90 30 1 1 90 1 89 2.61e-37 120
MS.gene50683.t1 MTR_4g072610 64.835 91 25 1 1 91 1 84 3.51e-37 120
MS.gene50683.t1 MTR_4g072820 65.556 90 25 1 1 90 1 84 4.88e-37 120
MS.gene50683.t1 MTR_4g072660 62.366 93 29 1 1 93 1 87 1.13e-36 119
MS.gene50683.t1 MTR_4g072940 73.611 72 19 0 22 93 53 124 1.59e-36 120
MS.gene50683.t1 MTR_4g072470 63.736 91 27 1 1 91 1 85 3.94e-36 117
MS.gene50683.t1 MTR_4g072650 63.736 91 26 1 1 91 1 84 4.57e-36 117
MS.gene50683.t1 MTR_3g084260 62.637 91 34 0 1 91 1 91 5.30e-36 117
MS.gene50683.t1 MTR_3g084190 69.892 93 27 1 1 93 1 92 7.16e-36 117
MS.gene50683.t1 MTR_4g072540 63.736 91 28 1 1 91 1 86 9.85e-36 116
MS.gene50683.t1 MTR_8g461420 67.742 93 29 1 1 93 1 92 3.28e-35 115
MS.gene50683.t1 MTR_3g084160 67.742 93 29 1 1 93 1 92 4.27e-35 115
MS.gene50683.t1 MTR_3g084210 67.742 93 29 1 1 93 1 92 5.38e-35 115
MS.gene50683.t1 MTR_3g084230 66.667 93 30 1 1 93 1 92 1.65e-34 114
MS.gene50683.t1 MTR_5g008360 67.105 76 25 0 18 93 8 83 2.32e-34 113
MS.gene50683.t1 MTR_4g072670 61.538 91 30 1 1 91 1 86 3.18e-34 112
MS.gene50683.t1 MTR_4g072510 62.637 91 29 1 1 91 1 86 3.92e-34 112
MS.gene50683.t1 MTR_4g072720 61.538 91 30 1 1 91 1 86 6.49e-34 112
MS.gene50683.t1 MTR_5g008370 76.119 67 16 0 25 91 24 90 9.21e-34 113
MS.gene50683.t1 MTR_4g072465 59.341 91 32 1 1 91 1 86 1.04e-33 111
MS.gene50683.t1 MTR_3g110045 72.222 72 20 0 22 93 80 151 1.21e-33 113
MS.gene50683.t1 MTR_4g072550 68.493 73 23 0 21 93 31 103 3.91e-33 110
MS.gene50683.t1 MTR_4g072590 67.073 82 27 0 10 91 4 85 6.45e-33 109
MS.gene50683.t1 MTR_4g072380 63.636 88 27 1 1 88 1 83 1.29e-32 108
MS.gene50683.t1 MTR_4g072690 63.736 91 27 1 1 91 1 85 5.71e-32 107
MS.gene50683.t1 MTR_5g008350 66.250 80 21 1 1 80 1 74 7.72e-32 106
MS.gene50683.t1 MTR_4g072910 68.657 67 21 0 25 91 34 100 1.28e-31 107
MS.gene50683.t1 MTR_4g072450 65.854 82 28 0 10 91 4 85 1.79e-31 105
MS.gene50683.t1 MTR_4g072770 69.697 66 20 0 26 91 15 80 3.87e-30 102
MS.gene50683.t1 MTR_3g084200 67.123 73 23 1 1 73 1 72 5.53e-30 102
MS.gene50683.t1 MTR_4g072980 53.261 92 41 2 1 90 1 92 1.17e-29 101
MS.gene50683.t1 MTR_3g117640 67.164 67 21 1 25 90 33 99 3.63e-29 100
MS.gene50683.t1 MTR_4g072560 65.152 66 22 1 26 90 32 97 1.70e-28 99.0
MS.gene50683.t1 MTR_4g072290 51.613 93 31 1 1 93 1 79 7.20e-28 96.7
MS.gene50683.t1 MTR_4g072930 47.059 102 43 2 1 91 9 110 1.35e-27 97.1
MS.gene50683.t1 MTR_4g072750 64.000 75 24 2 1 73 1 74 1.00e-26 94.0
MS.gene50683.t1 MTR_3g113310 48.864 88 39 2 8 90 19 105 4.62e-24 87.8
MS.gene50683.t1 MTR_3g110035 63.636 66 24 0 28 93 73 138 3.60e-23 86.7
MS.gene50683.t1 MTR_3g117610 60.938 64 24 1 25 88 44 106 3.62e-23 85.9
MS.gene50683.t1 MTR_6g077850 52.239 67 32 0 25 91 33 99 4.02e-23 85.5
MS.gene50683.t1 MTR_4g072190 51.807 83 36 2 8 90 21 99 1.01e-22 84.3
MS.gene50683.t1 MTR_5g021820 50.000 84 38 2 10 90 21 103 4.90e-21 80.1
MS.gene50683.t1 MTR_8g076040 50.000 84 38 2 8 88 20 102 1.00e-20 79.3
MS.gene50683.t1 MTR_4g124750 48.837 86 42 2 4 88 46 130 1.72e-20 80.1
MS.gene50683.t1 MTR_4g025830 52.778 72 30 2 23 91 76 146 3.29e-20 79.7
MS.gene50683.t1 MTR_4g072160 46.988 83 43 1 4 86 15 96 1.09e-19 77.8
MS.gene50683.t1 MTR_2g043960 50.000 84 40 2 4 86 53 135 1.37e-19 78.2
MS.gene50683.t1 MTR_8g461400 46.237 93 23 2 1 93 1 66 1.40e-18 72.8
MS.gene50683.t1 MTR_3g092220 41.176 85 47 2 9 91 16 99 1.98e-18 73.9
MS.gene50683.t1 MTR_4g005310 53.968 63 28 1 25 86 61 123 4.14e-18 73.9
MS.gene50683.t1 MTR_0388s0020 42.045 88 46 3 7 91 57 142 6.29e-18 73.6
MS.gene50683.t1 MTR_7g118010 55.556 63 25 2 26 86 23 84 6.33e-18 72.4
MS.gene50683.t1 MTR_4g072840 47.778 90 16 3 1 90 1 59 1.12e-16 67.8
MS.gene50683.t1 MTR_1g024085 45.679 81 32 2 4 84 26 94 1.13e-16 70.1
MS.gene50683.t1 MTR_8g026730 52.381 63 27 2 26 86 27 88 1.52e-16 68.9
MS.gene50683.t1 MTR_1g094750 41.379 87 47 2 9 92 30 115 1.25e-15 66.6
MS.gene50683.t1 MTR_1g009150 48.571 70 35 1 17 86 62 130 1.37e-15 68.2
MS.gene50683.t1 MTR_1g061570 51.613 62 29 1 25 86 18 78 2.88e-15 66.2
MS.gene50683.t1 MTR_3g097120 42.683 82 38 2 19 92 2 82 3.80e-15 65.1
MS.gene50683.t1 MTR_4g005320 49.180 61 30 1 26 86 70 129 2.59e-14 64.7
MS.gene50683.t1 MTR_3g109160 45.570 79 42 1 8 86 51 128 4.24e-14 63.9
MS.gene50683.t1 MTR_7g405740 43.056 72 40 1 12 83 53 123 6.74e-14 63.5
MS.gene50683.t1 MTR_1g059770 40.789 76 37 2 22 90 38 112 2.08e-13 61.2
MS.gene50683.t1 MTR_7g101740 46.875 64 33 1 25 88 39 101 2.56e-13 60.8
MS.gene50683.t1 MTR_8g461210 48.333 60 30 1 25 84 34 92 2.59e-13 60.8
MS.gene50683.t1 MTR_1g063950 45.763 59 31 1 25 83 47 104 3.26e-13 60.8
MS.gene50683.t1 MTR_8g094980 40.741 81 34 2 25 92 2 81 2.14e-12 58.2
MS.gene50683.t1 MTR_2g095430 43.939 66 32 2 25 86 44 108 2.26e-12 58.9
MS.gene50683.t1 MTR_2g066830 39.130 69 40 2 10 78 43 109 4.56e-12 58.2
MS.gene50683.t1 MTR_8g101520 43.939 66 36 1 27 92 51 115 5.09e-12 58.5
MS.gene50683.t1 MTR_1g063310 39.744 78 44 2 17 92 17 93 5.81e-12 57.0
MS.gene50683.t1 MTR_8g096440 45.070 71 36 2 13 83 34 101 8.38e-12 57.4
MS.gene50683.t1 MTR_7g101920 43.056 72 36 3 20 89 31 99 1.40e-11 56.2
MS.gene50683.t1 MTR_7g104940 40.323 62 36 1 29 90 53 113 2.56e-11 55.8
MS.gene50683.t1 MTR_7g104950 38.710 62 37 1 29 90 53 113 3.61e-11 55.5
MS.gene50683.t1 MTR_3g098970 31.818 88 57 2 8 93 33 119 5.37e-11 55.5
MS.gene50683.t1 MTR_4g021810 45.070 71 36 3 17 86 18 86 6.48e-11 54.3
MS.gene50683.t1 MTR_8g096470 45.614 57 30 1 27 83 47 102 7.55e-11 54.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50683.t1 AT2G21210 53.684 95 40 2 1 91 1 95 6.74e-32 107
MS.gene50683.t1 AT4G38840 72.059 68 18 1 25 91 32 99 2.01e-31 106
MS.gene50683.t1 AT2G21210 53.684 95 40 2 1 91 68 162 3.67e-31 107
MS.gene50683.t1 AT5G18080 62.667 75 27 1 17 90 15 89 4.07e-31 105
MS.gene50683.t1 AT5G18020 60.000 75 29 1 17 90 15 89 1.01e-30 104
MS.gene50683.t1 AT4G34770 71.014 69 18 1 25 91 34 102 4.04e-30 103
MS.gene50683.t1 AT5G18060 69.231 65 19 1 27 90 25 89 5.40e-30 102
MS.gene50683.t1 AT5G18050 66.667 66 21 1 26 90 24 89 8.10e-30 102
MS.gene50683.t1 AT3G03820 57.609 92 33 2 2 90 6 94 1.42e-29 101
MS.gene50683.t1 AT5G18030 66.667 66 21 1 26 90 22 87 2.67e-29 100
MS.gene50683.t1 AT5G18010 58.667 75 30 1 17 90 15 89 3.18e-29 100
MS.gene50683.t1 AT2G21200 61.111 72 27 1 20 90 15 86 1.05e-28 99.0
MS.gene50683.t1 AT4G38825 59.459 74 29 1 18 90 15 88 1.51e-28 99.0
MS.gene50683.t1 AT3G03840 61.972 71 25 1 22 90 23 93 4.40e-28 97.8
MS.gene50683.t1 AT4G38850 63.235 68 22 1 26 90 21 88 4.73e-28 97.4
MS.gene50683.t1 AT3G03850 57.471 87 30 2 6 90 10 91 1.09e-27 96.7
MS.gene50683.t1 AT4G34800 51.685 89 35 2 1 86 1 84 1.50e-26 94.0
MS.gene50683.t1 AT4G34810 51.087 92 39 3 4 90 27 117 3.02e-25 91.3
MS.gene50683.t1 AT4G34790 63.077 65 22 1 26 88 39 103 9.20e-25 89.7
MS.gene50683.t1 AT3G03830 59.420 69 25 1 25 90 23 91 1.55e-24 88.6
MS.gene50683.t1 AT4G13790 50.685 73 34 1 20 90 19 91 2.41e-23 85.9
MS.gene50683.t1 AT4G34780 57.971 69 26 1 26 91 28 96 2.72e-23 85.9
MS.gene50683.t1 AT1G75580 51.111 90 36 2 8 90 19 107 1.52e-22 84.3
MS.gene50683.t1 AT4G38860 50.000 86 40 1 8 90 19 104 5.50e-22 82.8
MS.gene50683.t1 AT2G21220 48.837 86 40 2 8 90 19 103 5.91e-22 82.4
MS.gene50683.t1 AT1G19830 57.576 66 27 1 25 90 47 111 6.29e-22 82.8
MS.gene50683.t1 AT4G34760 57.576 66 27 1 25 90 42 106 6.34e-22 82.4
MS.gene50683.t1 AT3G43120 58.065 62 25 1 25 86 78 138 3.95e-20 79.3
MS.gene50683.t1 AT2G16580 57.812 64 26 1 25 88 43 105 5.50e-20 77.8
MS.gene50683.t1 AT5G20810 58.730 63 25 1 24 86 77 138 1.65e-19 78.2
MS.gene50683.t1 AT2G18010 51.389 72 34 1 17 88 39 109 2.46e-19 76.3
MS.gene50683.t1 AT5G20810 58.730 63 25 1 24 86 77 138 4.54e-19 77.4
MS.gene50683.t1 AT4G36110 50.685 73 35 1 16 88 30 101 4.84e-19 75.1
MS.gene50683.t1 AT5G66260 53.030 66 30 1 26 91 34 98 1.39e-17 71.2
MS.gene50683.t1 AT2G45210 43.421 76 39 2 19 91 72 146 1.82e-17 72.8
MS.gene50683.t1 AT3G60690 48.529 68 31 2 26 90 88 154 1.09e-16 70.9
MS.gene50683.t1 AT3G20220 52.308 65 29 2 26 89 47 110 1.18e-16 69.3
MS.gene50683.t1 AT1G75590 50.000 72 31 2 13 84 33 99 2.05e-16 69.7
MS.gene50683.t1 AT2G46690 47.143 70 33 2 18 84 16 84 4.00e-16 68.2
MS.gene50683.t1 AT1G19840 48.000 75 34 2 10 84 29 98 8.30e-16 68.2
MS.gene50683.t1 AT5G10990 46.667 75 35 2 10 84 29 98 1.08e-15 67.8
MS.gene50683.t1 AT4G12410 47.826 69 32 2 26 91 76 143 2.85e-15 67.0
MS.gene50683.t1 AT3G51200 47.059 68 34 2 25 91 38 104 5.69e-15 64.7
MS.gene50683.t1 AT5G53590 42.466 73 36 2 25 92 45 116 6.43e-15 65.5
MS.gene50683.t1 AT4G00880 54.286 70 26 3 25 89 27 95 9.06e-15 64.7
MS.gene50683.t1 AT4G34750 43.750 64 35 1 25 88 36 98 2.45e-14 64.3
MS.gene50683.t1 AT4G34750 43.750 64 35 1 25 88 36 98 2.45e-14 64.3
MS.gene50683.t1 AT2G37030 46.032 63 33 1 25 87 48 109 2.59e-14 63.5
MS.gene50683.t1 AT4G22620 39.759 83 46 2 12 91 65 146 3.22e-14 64.3
MS.gene50683.t1 AT3G61900 40.909 88 45 3 12 93 16 102 3.38e-14 63.5
MS.gene50683.t1 AT4G09530 41.096 73 40 2 17 86 19 91 1.63e-13 61.2
MS.gene50683.t1 AT3G03847 58.000 50 19 2 34 83 80 127 1.31e-12 59.3
MS.gene50683.t1 AT4G31320 45.455 66 35 1 21 86 75 139 1.65e-12 60.5
MS.gene50683.t1 AT2G24400 46.269 67 35 1 26 92 64 129 1.78e-12 60.1
MS.gene50683.t1 AT1G56150 38.667 75 45 1 16 90 28 101 2.37e-12 58.2
MS.gene50683.t1 AT3G12830 42.105 76 39 2 15 90 43 113 2.78e-12 58.5
MS.gene50683.t1 AT3G53250 39.189 74 40 2 14 87 41 109 3.11e-12 58.2
MS.gene50683.t1 AT3G53250 39.189 74 40 2 14 87 72 140 4.28e-12 58.5
MS.gene50683.t1 AT3G09870 46.875 64 31 2 25 86 44 106 6.69e-12 57.0
MS.gene50683.t1 AT1G16510 41.538 65 37 1 26 90 54 117 8.40e-11 55.1

Find 22 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGGTAGATTCTTGTGTTT+TGG 0.227199 4.3:-33540174 None:intergenic
AATCAAGCAGAAGAAGAATT+TGG 0.271748 4.3:+33540305 MS.gene50683:CDS
AATGAGACGGTTTGTGATTC+CGG 0.275418 4.3:+33540244 MS.gene50683:CDS
TCAAGCGAGATGTGAGGTTT+TGG 0.324425 4.3:-33540379 None:intergenic
TAGACTGCTAGATAGCCTTT+TGG 0.353626 4.3:-33540209 None:intergenic
ATTTGGATATGATCATCCTA+TGG 0.456415 4.3:+33540322 MS.gene50683:CDS
GGCTATCTAGCAGTCTATGT+TGG 0.492148 4.3:+33540215 MS.gene50683:CDS
TTTAGTAATTCTTGAAACAA+TGG 0.528370 4.3:-33540284 None:intergenic
GGTTGGTTCAAGTATGATAC+CGG 0.535378 4.3:-33540263 None:intergenic
GTAATTCTTGAAACAATGGT+TGG 0.547064 4.3:-33540280 None:intergenic
TATCTAGCAGTCTATGTTGG+AGG 0.566084 4.3:+33540218 MS.gene50683:CDS
AAGGGATATGAAGTCCCAAA+AGG 0.574049 4.3:+33540194 MS.gene50683:CDS
TGGAATTCATCCTCGCTGCA+AGG 0.585771 4.3:-33540359 None:intergenic
TTTGGATATGATCATCCTAT+GGG 0.586077 4.3:+33540323 MS.gene50683:CDS
TTCTAATAAGCTTTGCTATG+CGG 0.591314 4.3:-33540139 None:intergenic
GGATATGATCATCCTATGGG+TGG 0.645721 4.3:+33540326 MS.gene50683:CDS
CAAAACACAAGAATCTACCA+AGG 0.648832 4.3:+33540175 MS.gene50683:CDS
ATGTTGGAGGTAGAATGAGA+CGG 0.650884 4.3:+33540231 MS.gene50683:CDS
GGAATTGTGAGACCACCCAT+AGG 0.657747 4.3:-33540338 None:intergenic
GTTCACTCAAGCGAGATGTG+AGG 0.677051 4.3:-33540385 None:intergenic
AAAACACAAGAATCTACCAA+GGG 0.683751 4.3:+33540176 MS.gene50683:CDS
TCTCACAATTCCTTGCAGCG+AGG 0.736678 4.3:+33540349 MS.gene50683:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTAGTAATTCTTGAAACAA+TGG - chr4.3:33540287-33540306 None:intergenic 20.0%
AAAACACAAGAATCTACCAA+GGG + chr4.3:33540176-33540195 MS.gene50683:CDS 30.0%
AATCAAGCAGAAGAAGAATT+TGG + chr4.3:33540305-33540324 MS.gene50683:CDS 30.0%
ATTTGGATATGATCATCCTA+TGG + chr4.3:33540322-33540341 MS.gene50683:CDS 30.0%
GTAATTCTTGAAACAATGGT+TGG - chr4.3:33540283-33540302 None:intergenic 30.0%
TTTGGATATGATCATCCTAT+GGG + chr4.3:33540323-33540342 MS.gene50683:CDS 30.0%
! TTCTAATAAGCTTTGCTATG+CGG - chr4.3:33540142-33540161 None:intergenic 30.0%
!!! TCTTGTGTTTTGGAAAATGA+TGG - chr4.3:33540167-33540186 None:intergenic 30.0%
CAAAACACAAGAATCTACCA+AGG + chr4.3:33540175-33540194 MS.gene50683:CDS 35.0%
! CTTGGTAGATTCTTGTGTTT+TGG - chr4.3:33540177-33540196 None:intergenic 35.0%
AAGGGATATGAAGTCCCAAA+AGG + chr4.3:33540194-33540213 MS.gene50683:CDS 40.0%
AATGAGACGGTTTGTGATTC+CGG + chr4.3:33540244-33540263 MS.gene50683:CDS 40.0%
ATGTTGGAGGTAGAATGAGA+CGG + chr4.3:33540231-33540250 MS.gene50683:CDS 40.0%
TATCTAGCAGTCTATGTTGG+AGG + chr4.3:33540218-33540237 MS.gene50683:CDS 40.0%
! AGACTGCTAGATAGCCTTTT+GGG - chr4.3:33540211-33540230 None:intergenic 40.0%
! GGTTGGTTCAAGTATGATAC+CGG - chr4.3:33540266-33540285 None:intergenic 40.0%
! TAGACTGCTAGATAGCCTTT+TGG - chr4.3:33540212-33540231 None:intergenic 40.0%
!!! TTTTGGGACTTCATATCCCT+TGG - chr4.3:33540195-33540214 None:intergenic 40.0%
GGATATGATCATCCTATGGG+TGG + chr4.3:33540326-33540345 MS.gene50683:CDS 45.0%
GGCTATCTAGCAGTCTATGT+TGG + chr4.3:33540215-33540234 MS.gene50683:CDS 45.0%
! TCAAGCGAGATGTGAGGTTT+TGG - chr4.3:33540382-33540401 None:intergenic 45.0%
GGAATTGTGAGACCACCCAT+AGG - chr4.3:33540341-33540360 None:intergenic 50.0%
GTTCACTCAAGCGAGATGTG+AGG - chr4.3:33540388-33540407 None:intergenic 50.0%
TCTCACAATTCCTTGCAGCG+AGG + chr4.3:33540349-33540368 MS.gene50683:CDS 50.0%
TGGAATTCATCCTCGCTGCA+AGG - chr4.3:33540362-33540381 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 33540131 33540412 33540131 ID=MS.gene50683
chr4.3 mRNA 33540131 33540412 33540131 ID=MS.gene50683.t1;Parent=MS.gene50683
chr4.3 exon 33540131 33540412 33540131 ID=MS.gene50683.t1.exon1;Parent=MS.gene50683.t1
chr4.3 CDS 33540131 33540412 33540131 ID=cds.MS.gene50683.t1;Parent=MS.gene50683.t1
Gene Sequence

>MS.gene50683

ATGGGTTTCCGCATAGCAAAGCTTATTAGAATGCCATCATTTTCCAAAACACAAGAATCTACCAAGGGATATGAAGTCCCAAAAGGCTATCTAGCAGTCTATGTTGGAGGTAGAATGAGACGGTTTGTGATTCCGGTATCATACTTGAACCAACCATTGTTTCAAGAATTACTAAATCAAGCAGAAGAAGAATTTGGATATGATCATCCTATGGGTGGTCTCACAATTCCTTGCAGCGAGGATGAATTCCAAAACCTCACATCTCGCTTGAGTGAACTGTAA
Protein sequence

>MS.gene50683.t1

MGFRIAKLIRMPSFSKTQESTKGYEVPKGYLAVYVGGRMRRFVIPVSYLNQPLFQELLNQAEEEFGYDHPMGGLTIPCSEDEFQNLTSRLSEL