AlfalfaGEDB | MS.gene50685

M. sativa cultivar XinJiangDaYe / MS.gene50685

KEGG: K14488
GO: GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
PFAM: PF02519.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50685.t1	RHN61450.1	92.5	93	7	0	1	93	1	93	2.20E-43	184.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50685.t1	P33083	81.3	91	16	1	1	91	1	90	5.1e-36	151.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50685.t1	A0A396I775	92.5	93	7	0	1	93	1	93	1.6e-43	184.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene50685	MS.gene057818	PPI
MS.gene50685	MS.gene68164	PPI
MS.gene50685	MS.gene68759	PPI
MS.gene50685	MS.gene79889	PPI
MS.gene50685	MS.gene045368	PPI
MS.gene50685	MS.gene031200	PPI
MS.gene50763	MS.gene50685	PPI
MS.gene50685	MS.gene014222	PPI
MS.gene50685	MS.gene79888	PPI
MS.gene045365	MS.gene50685	PPI
MS.gene50685	MS.gene75676	PPI
MS.gene50685	MS.gene75678	PPI
MS.gene50685	MS.gene031199	PPI
MS.gene50659	MS.gene50685	PPI
MS.gene50685	MS.gene50687	PPI
MS.gene50685	MS.gene014223	PPI
MS.gene75316	MS.gene50685	PPI
MS.gene50685	MS.gene79885	PPI
MS.gene057790	MS.gene50685	PPI
MS.gene003624	MS.gene50685	PPI
MS.gene50685	MS.gene50764	PPI
MS.gene77529	MS.gene50685	PPI
MS.gene014667	MS.gene50685	PPI
MS.gene50685	MS.gene003577	PPI
MS.gene50685	MS.gene057801	PPI
MS.gene057778	MS.gene50685	PPI
MS.gene75673	MS.gene50685	PPI
MS.gene045366	MS.gene50685	PPI
MS.gene062963	MS.gene50685	PPI
MS.gene50685	MS.gene014670	PPI
MS.gene75674	MS.gene50685	PPI
MS.gene79890	MS.gene50685	PPI
MS.gene003615	MS.gene50685	PPI
MS.gene50685	MS.gene75314	PPI
MS.gene50685	MS.gene01764	PPI
MS.gene003602	MS.gene50685	PPI
MS.gene23372	MS.gene50685	PPI
MS.gene50685	MS.gene75679	PPI
MS.gene50685	MS.gene40325	PPI
MS.gene003627	MS.gene50685	PPI
MS.gene062984	MS.gene50685	PPI
MS.gene50685	MS.gene68760	PPI
MS.gene40327	MS.gene50685	PPI
MS.gene031201	MS.gene50685	PPI
MS.gene50685	MS.gene031198	PPI
MS.gene50685	MS.gene057797	PPI
MS.gene50685	MS.gene003614	PPI
MS.gene50685	MS.gene77528	PPI
MS.gene50685	MS.gene014671	PPI
MS.gene50685	MS.gene003596	PPI
MS.gene50685	MS.gene23371	PPI
MS.gene045362	MS.gene50685	PPI
MS.gene50685	MS.gene75672	PPI
MS.gene77523	MS.gene50685	PPI
MS.gene003587	MS.gene50685	PPI
MS.gene50685	MS.gene50654	PPI
MS.gene50685	MS.gene062974	PPI
MS.gene50685	MS.gene79886	PPI
MS.gene003590	MS.gene50685	PPI
MS.gene063112	MS.gene50685	PPI
MS.gene50685	MS.gene003583	PPI
MS.gene50685	MS.gene062951	PPI
MS.gene50685	MS.gene77527	PPI
MS.gene50685	MS.gene057795	PPI
MS.gene50685	MS.gene68162	PPI
MS.gene50685	MS.gene045363	PPI
MS.gene50685	MS.gene75675	PPI
MS.gene50685	MS.gene79887	PPI
MS.gene062973	MS.gene50685	PPI
MS.gene77526	MS.gene50685	PPI
MS.gene047517	MS.gene50685	PPI
MS.gene50685	MS.gene50652	PPI
MS.gene50685	MS.gene68761	PPI
MS.gene045360	MS.gene50685	PPI
MS.gene50685	MS.gene68165	PPI
MS.gene50685	MS.gene50684	PPI
MS.gene75677	MS.gene50685	PPI
MS.gene50685	MS.gene014668	PPI
MS.gene50685	MS.gene50765	PPI
MS.gene045361	MS.gene50685	PPI
MS.gene50675	MS.gene50685	PPI
MS.gene003591	MS.gene50685	PPI
MS.gene062979	MS.gene50685	PPI
MS.gene062943	MS.gene50685	PPI
MS.gene50685	MS.gene79891	PPI
MS.gene50685	MS.gene045367	PPI
MS.gene01358	MS.gene50685	PPI
MS.gene50685	MS.gene045364	PPI
MS.gene003608	MS.gene50685	PPI
MS.gene40326	MS.gene50685	PPI
MS.gene062952	MS.gene50685	PPI
MS.gene50685	MS.gene014669	PPI
MS.gene50685	MS.gene057808	PPI
MS.gene50685	MS.gene002380	PPI
MS.gene50685	MS.gene77524	PPI
MS.gene50676	MS.gene50685	PPI
MS.gene23370	MS.gene50685	PPI
MS.gene50766	MS.gene50685	PPI
MS.gene003594	MS.gene50685	PPI
MS.gene50665	MS.gene50685	PPI
MS.gene50677	MS.gene50685	PPI
MS.gene50685	MS.gene68163	PPI
MS.gene003625	MS.gene50685	PPI
MS.gene003586	MS.gene50685	PPI
MS.gene50685	MS.gene75315	PPI
MS.gene062988	MS.gene50685	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50685.t1	MTR_4g072270	94.624	93	5	0	1	93	1	93	4.79e-62	183
MS.gene50685.t1	MTR_4g072310	91.398	93	8	0	1	93	1	93	4.21e-61	181
MS.gene50685.t1	MTR_4g072880	91.398	93	8	0	1	93	1	93	9.92e-61	180
MS.gene50685.t1	MTR_4g072230	84.946	93	12	1	1	93	1	91	5.49e-55	165
MS.gene50685.t1	MTR_4g072250	82.796	93	15	1	1	93	1	92	2.69e-53	161
MS.gene50685.t1	MTR_4g072860	80.645	93	18	0	1	93	1	93	9.93e-53	159
MS.gene50685.t1	MTR_3g084150	74.194	93	23	1	1	93	1	92	3.95e-49	150
MS.gene50685.t1	MTR_3g084170	75.269	93	22	1	1	93	1	92	1.05e-48	149
MS.gene50685.t1	MTR_3g084180	73.118	93	24	1	1	93	1	92	3.83e-48	148
MS.gene50685.t1	MTR_3g084240	74.194	93	23	1	1	93	1	92	7.56e-48	147
MS.gene50685.t1	MTR_3g110038	74.194	93	23	1	1	93	1	92	9.42e-48	147
MS.gene50685.t1	MTR_4g072500	72.043	93	25	1	1	93	1	92	1.11e-47	147
MS.gene50685.t1	MTR_8g461410	70.968	93	26	1	1	93	1	92	2.03e-47	146
MS.gene50685.t1	MTR_4g072640	74.725	91	22	1	1	91	1	90	2.79e-47	146
MS.gene50685.t1	MTR_3g110032	70.968	93	27	0	1	93	1	93	5.03e-47	145
MS.gene50685.t1	MTR_4g072480	73.118	93	24	1	1	93	1	92	1.27e-46	144
MS.gene50685.t1	MTR_4g050700	69.892	93	28	0	1	93	1	93	1.84e-46	144
MS.gene50685.t1	MTR_2g012410	69.892	93	28	0	1	93	1	93	1.84e-46	144
MS.gene50685.t1	MTR_4g072620	73.118	93	24	1	1	93	1	92	2.40e-46	144
MS.gene50685.t1	MTR_4g072680	73.118	93	24	1	1	93	1	92	3.80e-46	143
MS.gene50685.t1	MTR_3g084220	72.043	93	25	1	1	93	1	92	3.97e-46	143
MS.gene50685.t1	MTR_4g072240	71.429	91	26	0	1	91	1	91	5.92e-46	142
MS.gene50685.t1	MTR_4g072460	73.118	93	24	1	1	93	1	92	5.96e-46	142
MS.gene50685.t1	MTR_3g117630	73.333	90	24	0	4	93	3	92	1.73e-45	141
MS.gene50685.t1	MTR_4g072370	73.626	91	21	1	1	91	1	88	1.86e-45	141
MS.gene50685.t1	MTR_4g072530	70.330	91	26	1	1	91	1	90	2.44e-45	141
MS.gene50685.t1	MTR_4g072730	71.739	92	25	1	1	92	1	91	3.02e-45	140
MS.gene50685.t1	MTR_4g072850	75.532	94	22	1	1	93	1	94	5.50e-45	140
MS.gene50685.t1	MTR_4g072570	70.968	93	26	1	1	93	1	92	8.67e-45	139
MS.gene50685.t1	MTR_4g072220	66.667	93	31	0	1	93	1	93	3.48e-44	138
MS.gene50685.t1	MTR_4g072870	68.132	91	29	0	1	91	1	91	4.82e-44	137
MS.gene50685.t1	MTR_3g084260	66.667	93	31	0	1	93	1	93	2.68e-43	136
MS.gene50685.t1	MTR_4g072300	67.033	91	30	0	1	91	1	91	3.25e-43	135
MS.gene50685.t1	MTR_4g072890	68.132	91	29	0	1	91	1	91	5.21e-43	135
MS.gene50685.t1	MTR_4g072260	66.667	93	31	0	1	93	1	93	6.52e-43	135
MS.gene50685.t1	MTR_4g072660	68.817	93	23	1	1	93	1	87	1.22e-42	134
MS.gene50685.t1	MTR_3g084250	66.667	93	30	1	1	93	1	92	1.79e-42	134
MS.gene50685.t1	MTR_4g072740	71.429	91	20	1	1	91	1	85	2.50e-42	133
MS.gene50685.t1	MTR_3g110042	71.591	88	24	1	1	88	1	87	3.01e-42	133
MS.gene50685.t1	MTR_4g072580	69.231	91	22	1	1	91	1	85	3.12e-42	133
MS.gene50685.t1	MTR_4g072290	68.817	93	15	1	1	93	1	79	6.49e-42	132
MS.gene50685.t1	MTR_4g072490	69.231	91	22	1	1	91	1	85	3.08e-41	130
MS.gene50685.t1	MTR_4g072830	70.000	90	24	1	1	90	1	87	3.30e-41	130
MS.gene50685.t1	MTR_4g072330	64.835	91	29	1	1	91	1	88	4.35e-41	130
MS.gene50685.t1	MTR_4g072470	69.231	91	22	1	1	91	1	85	8.36e-41	129
MS.gene50685.t1	MTR_4g072940	70.652	92	26	1	3	93	33	124	2.06e-40	129
MS.gene50685.t1	MTR_5g008360	72.152	79	22	0	15	93	5	83	9.31e-40	127
MS.gene50685.t1	MTR_4g072610	69.231	91	21	1	1	91	1	84	1.08e-39	126
MS.gene50685.t1	MTR_4g072465	65.934	91	26	1	1	91	1	86	1.44e-39	126
MS.gene50685.t1	MTR_5g008370	67.368	95	24	2	1	92	1	91	2.93e-39	127
MS.gene50685.t1	MTR_4g072630	64.835	91	27	1	1	91	1	86	3.63e-38	122
MS.gene50685.t1	MTR_3g084210	66.667	93	30	1	1	93	1	92	3.67e-38	123
MS.gene50685.t1	MTR_3g084190	68.817	93	28	1	1	93	1	92	5.94e-38	122
MS.gene50685.t1	MTR_4g072820	66.304	92	23	2	1	91	1	85	6.35e-38	122
MS.gene50685.t1	MTR_4g072600	65.909	88	24	1	1	88	1	82	9.28e-38	122
MS.gene50685.t1	MTR_4g072380	68.478	92	22	2	1	91	1	86	1.22e-37	121
MS.gene50685.t1	MTR_4g072540	65.934	91	26	1	1	91	1	86	1.34e-37	121
MS.gene50685.t1	MTR_4g072550	73.973	73	19	0	21	93	31	103	3.03e-37	121
MS.gene50685.t1	MTR_4g072650	64.835	91	25	1	1	91	1	84	3.36e-37	120
MS.gene50685.t1	MTR_8g461420	67.033	91	29	1	1	91	1	90	4.88e-37	120
MS.gene50685.t1	MTR_4g072670	64.835	91	27	1	1	91	1	86	1.31e-36	119
MS.gene50685.t1	MTR_3g084230	67.033	91	29	1	1	91	1	90	1.34e-36	119
MS.gene50685.t1	MTR_3g084160	65.591	93	31	1	1	93	1	92	4.42e-36	117
MS.gene50685.t1	MTR_4g072720	77.143	70	16	0	22	91	17	86	6.08e-36	117
MS.gene50685.t1	MTR_3g110045	67.033	91	29	1	3	93	62	151	2.15e-35	118
MS.gene50685.t1	MTR_4g072510	62.637	91	29	1	1	91	1	86	1.21e-34	114
MS.gene50685.t1	MTR_4g072590	67.033	91	24	1	1	91	1	85	1.93e-34	113
MS.gene50685.t1	MTR_3g084200	68.493	73	22	1	1	73	1	72	7.03e-33	110
MS.gene50685.t1	MTR_5g008350	65.000	80	22	1	1	80	1	74	3.58e-32	107
MS.gene50685.t1	MTR_4g072910	57.447	94	34	2	4	91	7	100	5.28e-32	107
MS.gene50685.t1	MTR_4g072690	61.538	91	29	1	1	91	1	85	9.99e-32	106
MS.gene50685.t1	MTR_4g072560	58.333	84	27	2	9	91	22	98	3.10e-31	105
MS.gene50685.t1	MTR_4g072450	61.538	91	29	1	1	91	1	85	5.58e-31	104
MS.gene50685.t1	MTR_4g072980	53.125	96	41	3	1	93	1	95	1.05e-30	104
MS.gene50685.t1	MTR_4g072770	68.182	66	21	0	26	91	15	80	6.33e-29	99.4
MS.gene50685.t1	MTR_3g110035	60.256	78	30	1	16	93	62	138	1.92e-28	100
MS.gene50685.t1	MTR_3g117640	60.870	69	26	1	25	92	33	101	5.07e-28	97.8
MS.gene50685.t1	MTR_4g072750	55.814	86	33	3	1	82	1	85	8.99e-28	96.7
MS.gene50685.t1	MTR_8g461400	54.839	93	15	2	1	93	1	66	2.74e-27	94.7
MS.gene50685.t1	MTR_3g113310	61.538	65	24	1	24	88	40	103	3.68e-27	95.9
MS.gene50685.t1	MTR_4g072280	56.000	75	33	0	1	75	1	75	2.46e-26	92.8
MS.gene50685.t1	MTR_3g117610	63.077	65	23	1	24	88	43	106	3.19e-26	93.6
MS.gene50685.t1	MTR_6g077850	46.591	88	46	1	4	91	13	99	5.58e-26	92.4
MS.gene50685.t1	MTR_4g072190	55.696	79	31	2	10	88	23	97	6.09e-26	92.4
MS.gene50685.t1	MTR_5g021820	59.375	64	25	1	25	88	39	101	1.06e-23	87.0
MS.gene50685.t1	MTR_8g076040	59.375	64	25	1	25	88	40	102	3.04e-23	85.9
MS.gene50685.t1	MTR_4g072930	48.235	85	40	1	11	91	26	110	4.67e-23	85.5
MS.gene50685.t1	MTR_4g072840	47.826	92	15	3	1	91	1	60	2.56e-19	74.7
MS.gene50685.t1	MTR_4g124750	50.000	74	35	2	15	88	59	130	2.13e-17	72.4
MS.gene50685.t1	MTR_2g043960	54.839	62	27	1	25	86	75	135	3.21e-17	72.0
MS.gene50685.t1	MTR_4g005310	49.296	71	32	2	17	86	56	123	9.32e-17	70.5
MS.gene50685.t1	MTR_1g024085	51.429	70	30	2	15	84	29	94	1.51e-16	69.7
MS.gene50685.t1	MTR_7g118010	50.794	63	28	2	26	86	23	84	5.08e-15	65.1
MS.gene50685.t1	MTR_3g092220	45.714	70	35	2	17	84	24	92	6.88e-15	64.7
MS.gene50685.t1	MTR_1g009150	52.459	61	28	1	26	86	71	130	7.68e-15	66.2
MS.gene50685.t1	MTR_0388s0020	44.444	72	36	2	20	88	69	139	9.40e-15	65.5
MS.gene50685.t1	MTR_1g094750	50.000	62	30	1	25	86	49	109	1.29e-14	64.3
MS.gene50685.t1	MTR_4g025830	45.588	68	33	2	26	90	79	145	1.61e-14	65.1
MS.gene50685.t1	MTR_3g109160	43.373	83	46	1	10	92	53	134	3.11e-14	64.3
MS.gene50685.t1	MTR_1g061570	41.176	85	46	2	8	92	4	84	3.58e-14	63.2
MS.gene50685.t1	MTR_1g059770	42.857	63	35	1	26	88	49	110	1.55e-13	61.6
MS.gene50685.t1	MTR_4g005320	47.541	61	31	1	26	86	70	129	1.59e-13	62.4
MS.gene50685.t1	MTR_8g026730	46.032	63	31	2	26	86	27	88	1.64e-13	61.2
MS.gene50685.t1	MTR_8g461210	39.560	91	51	3	4	92	14	102	2.11e-13	61.2
MS.gene50685.t1	MTR_1g063950	43.478	69	38	1	15	83	37	104	2.12e-13	61.2
MS.gene50685.t1	MTR_4g072160	48.438	64	32	1	23	86	34	96	2.89e-13	61.2
MS.gene50685.t1	MTR_7g101740	45.312	64	34	1	25	88	39	101	1.25e-12	58.9
MS.gene50685.t1	MTR_3g105900	72.222	36	10	0	25	60	1171	1206	2.08e-12	61.6
MS.gene50685.t1	MTR_7g104940	41.667	60	34	1	29	88	53	111	5.64e-12	57.8
MS.gene50685.t1	MTR_7g104950	32.927	82	53	2	8	88	31	111	9.65e-12	57.0
MS.gene50685.t1	MTR_2g095430	41.333	75	37	3	10	78	27	100	2.42e-11	56.2
MS.gene50685.t1	MTR_3g098970	32.099	81	53	2	13	93	41	119	3.82e-11	55.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50685.t1	AT4G38840	63.529	85	30	1	8	91	15	99	1.11e-34	114
MS.gene50685.t1	AT5G18020	65.789	76	25	1	17	91	15	90	5.52e-33	110
MS.gene50685.t1	AT5G18030	69.444	72	21	1	21	91	17	88	4.59e-32	107
MS.gene50685.t1	AT3G03840	68.493	73	21	1	22	92	23	95	1.06e-31	107
MS.gene50685.t1	AT5G18060	64.474	76	26	1	17	91	15	90	1.95e-31	106
MS.gene50685.t1	AT5G18080	64.474	76	26	1	17	91	15	90	2.57e-31	105
MS.gene50685.t1	AT5G18050	61.842	76	28	1	17	91	15	90	9.25e-31	104
MS.gene50685.t1	AT5G18010	63.158	76	27	1	17	91	15	90	9.46e-31	104
MS.gene50685.t1	AT2G21200	67.606	71	22	1	21	90	16	86	1.49e-30	103
MS.gene50685.t1	AT2G21210	60.811	74	27	1	20	91	22	95	1.16e-29	102
MS.gene50685.t1	AT4G38825	65.278	72	24	1	21	91	18	89	1.75e-29	101
MS.gene50685.t1	AT3G03850	69.118	68	19	1	27	92	26	93	4.22e-29	100
MS.gene50685.t1	AT2G21210	60.811	74	27	1	20	91	89	162	8.18e-29	101
MS.gene50685.t1	AT3G03820	53.191	94	38	2	2	92	6	96	2.35e-28	98.6
MS.gene50685.t1	AT4G38850	63.380	71	23	1	22	89	17	87	5.64e-28	97.4
MS.gene50685.t1	AT3G03830	56.818	88	31	2	11	91	5	92	7.72e-28	97.1
MS.gene50685.t1	AT4G34770	57.647	85	31	2	10	92	22	103	1.66e-27	96.7
MS.gene50685.t1	AT4G38860	63.077	65	24	0	24	88	38	102	4.34e-26	93.2
MS.gene50685.t1	AT4G34810	58.333	72	25	2	17	83	38	109	2.04e-25	91.7
MS.gene50685.t1	AT2G21220	61.538	65	24	1	24	88	38	101	3.52e-25	90.9
MS.gene50685.t1	AT4G34760	61.538	65	24	1	24	88	41	104	4.96e-25	90.5
MS.gene50685.t1	AT4G34800	63.934	61	19	1	26	83	21	81	5.93e-25	89.7
MS.gene50685.t1	AT1G75580	61.538	65	24	1	24	88	42	105	8.71e-25	89.7
MS.gene50685.t1	AT4G13790	52.778	72	32	1	20	89	19	90	1.33e-24	89.0
MS.gene50685.t1	AT4G34780	45.161	93	45	2	4	90	3	95	6.10e-22	82.4
MS.gene50685.t1	AT2G16580	56.923	65	27	1	24	88	42	105	6.87e-22	82.4
MS.gene50685.t1	AT1G19830	55.882	68	29	1	21	88	43	109	1.03e-21	82.4
MS.gene50685.t1	AT4G36110	56.061	66	28	1	25	90	39	103	1.19e-21	81.6
MS.gene50685.t1	AT3G20220	50.649	77	36	2	15	90	36	111	2.72e-21	81.3
MS.gene50685.t1	AT4G34790	52.308	65	29	1	26	88	39	103	9.11e-21	79.7
MS.gene50685.t1	AT2G18010	54.545	66	29	1	25	90	47	111	2.37e-20	78.6
MS.gene50685.t1	AT5G66260	53.030	66	30	1	26	91	34	98	3.02e-18	73.2
MS.gene50685.t1	AT3G43120	53.226	62	28	1	25	86	78	138	2.26e-17	72.4
MS.gene50685.t1	AT5G20810	54.839	62	27	1	25	86	78	138	8.54e-17	70.9
MS.gene50685.t1	AT5G20810	54.839	62	27	1	25	86	78	138	1.70e-16	70.9
MS.gene50685.t1	AT2G46690	41.667	84	44	3	13	92	10	92	1.18e-15	67.0
MS.gene50685.t1	AT3G51200	50.000	64	30	2	21	83	34	96	2.58e-15	65.9
MS.gene50685.t1	AT4G34750	44.595	74	37	2	15	88	29	98	4.17e-15	66.2
MS.gene50685.t1	AT4G34750	44.595	74	37	2	15	88	29	98	4.17e-15	66.2
MS.gene50685.t1	AT1G75590	46.575	73	34	2	12	84	32	99	8.08e-15	65.5
MS.gene50685.t1	AT4G31320	52.459	61	28	1	26	86	80	139	1.27e-14	65.9
MS.gene50685.t1	AT5G10990	44.595	74	39	2	11	84	27	98	2.82e-14	63.9
MS.gene50685.t1	AT3G61900	44.286	70	35	2	25	91	29	97	3.88e-14	63.5
MS.gene50685.t1	AT2G24400	54.098	61	27	1	26	86	64	123	4.61e-14	64.3
MS.gene50685.t1	AT2G45210	41.096	73	39	2	21	90	74	145	4.81e-14	63.9
MS.gene50685.t1	AT1G19840	42.857	84	43	2	1	84	20	98	9.41e-14	62.8
MS.gene50685.t1	AT5G53590	35.484	93	54	2	1	88	21	112	2.28e-13	61.6
MS.gene50685.t1	AT4G00880	50.794	63	28	2	26	86	28	89	6.97e-13	60.1
MS.gene50685.t1	AT3G60690	42.029	69	36	2	26	91	88	155	9.42e-13	60.5
MS.gene50685.t1	AT2G37030	47.458	59	30	1	25	83	48	105	1.04e-12	59.7
MS.gene50685.t1	AT2G36210	38.356	73	45	0	16	88	44	116	1.33e-11	57.0
MS.gene50685.t1	AT4G09530	41.935	62	33	2	26	84	28	89	8.79e-11	53.9

Find 25 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGTTGGCTCAAGTATGATAC+TGG	0.342744	4.3:+33554614	None:intergenic
CATATAACTTCTCGCTTCAA+TGG	0.354508	4.3:-33554494	MS.gene50685:CDS
AGTCAAGTTGAGGAAGAGTT+TGG	0.373802	4.3:-33554572	MS.gene50685:CDS
CTCAGCAAATCTTGAAATGA+AGG	0.381225	4.3:+33554593	None:intergenic
ATATAACTTCTCGCTTCAAT+GGG	0.506633	4.3:-33554493	MS.gene50685:CDS
GCAAATCTTGAAATGAAGGT+TGG	0.509792	4.3:+33554597	None:intergenic
GGATATCTTGCAGTATATGT+TGG	0.514243	4.3:-33554662	MS.gene50685:CDS
AAAAGCTGTGGACGTGCCAA+AGG	0.523365	4.3:-33554684	MS.gene50685:CDS
TGGCCTCACCATTCCTTGCA+CGG	0.538434	4.3:-33554531	MS.gene50685:CDS
GACATCTTCCGTGCAAGGAA+TGG	0.540062	4.3:+33554523	None:intergenic
TTTCCCTGGTATCATCAGAA+AGG	0.541055	4.3:-33554735	MS.gene50685:CDS
TTTGGATATGATCATCCAAT+GGG	0.545525	4.3:-33554554	MS.gene50685:CDS
TATACTGCAAGATATCCCTT+TGG	0.554191	4.3:+33554668	None:intergenic
GGAATGGTGAGGCCACCCAT+TGG	0.562484	4.3:+33554539	None:intergenic
GATGCCTTTCTGATGATACC+AGG	0.565258	4.3:+33554731	None:intergenic
TGAAAGACATCTTCCGTGCA+AGG	0.572644	4.3:+33554518	None:intergenic
GTTTGGATATGATCATCCAA+TGG	0.573812	4.3:-33554555	MS.gene50685:CDS
AGATTTGCTGAGTCAAGTTG+AGG	0.574582	4.3:-33554582	MS.gene50685:CDS
AAAGCTGTGGACGTGCCAAA+GGG	0.577804	4.3:-33554683	MS.gene50685:CDS
CCGACCAGCTTCAAAAGCTG+TGG	0.627657	4.3:-33554696	MS.gene50685:CDS
GGATATGATCATCCAATGGG+TGG	0.648071	4.3:-33554551	MS.gene50685:CDS
ATGCCTTTCTGATGATACCA+GGG	0.648151	4.3:+33554732	None:intergenic
GTTGGCTCAAGTATGATACT+GGG	0.652870	4.3:+33554615	None:intergenic
CTTCCGTGCAAGGAATGGTG+AGG	0.671810	4.3:+33554528	None:intergenic
ATGTTGGAGAGAAACAGACA+CGG	0.798276	4.3:-33554646	MS.gene50685:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ATATAACTTCTCGCTTCAAT+GGG	-	chr4.3:33554740-33554759	MS.gene50685:CDS	30.0%
	TTTGGATATGATCATCCAAT+GGG	-	chr4.3:33554679-33554698	MS.gene50685:CDS	30.0%
	CATATAACTTCTCGCTTCAA+TGG	-	chr4.3:33554739-33554758	MS.gene50685:CDS	35.0%
	CTCAGCAAATCTTGAAATGA+AGG	+	chr4.3:33554643-33554662	None:intergenic	35.0%
	GCAAATCTTGAAATGAAGGT+TGG	+	chr4.3:33554639-33554658	None:intergenic	35.0%
	GGATATCTTGCAGTATATGT+TGG	-	chr4.3:33554571-33554590	MS.gene50685:CDS	35.0%
	GTTTGGATATGATCATCCAA+TGG	-	chr4.3:33554678-33554697	MS.gene50685:CDS	35.0%
	TATACTGCAAGATATCCCTT+TGG	+	chr4.3:33554568-33554587	None:intergenic	35.0%
	ATGCCTTTCTGATGATACCA+GGG	+	chr4.3:33554504-33554523	None:intergenic	40.0%
	ATGTTGGAGAGAAACAGACA+CGG	-	chr4.3:33554587-33554606	MS.gene50685:CDS	40.0%
	TTTCCCTGGTATCATCAGAA+AGG	-	chr4.3:33554498-33554517	MS.gene50685:CDS	40.0%
!	GTTGGCTCAAGTATGATACT+GGG	+	chr4.3:33554621-33554640	None:intergenic	40.0%
!!	AGATTTGCTGAGTCAAGTTG+AGG	-	chr4.3:33554651-33554670	MS.gene50685:CDS	40.0%
!!	AGTCAAGTTGAGGAAGAGTT+TGG	-	chr4.3:33554661-33554680	MS.gene50685:CDS	40.0%
	GATGCCTTTCTGATGATACC+AGG	+	chr4.3:33554505-33554524	None:intergenic	45.0%
	GGATATGATCATCCAATGGG+TGG	-	chr4.3:33554682-33554701	MS.gene50685:CDS	45.0%
	TGAAAGACATCTTCCGTGCA+AGG	+	chr4.3:33554718-33554737	None:intergenic	45.0%
!	GGTTGGCTCAAGTATGATAC+TGG	+	chr4.3:33554622-33554641	None:intergenic	45.0%
	AAAAGCTGTGGACGTGCCAA+AGG	-	chr4.3:33554549-33554568	MS.gene50685:CDS	50.0%
	AAAGCTGTGGACGTGCCAAA+GGG	-	chr4.3:33554550-33554569	MS.gene50685:CDS	50.0%
	GACATCTTCCGTGCAAGGAA+TGG	+	chr4.3:33554713-33554732	None:intergenic	50.0%
!!!	ACGTCCACAGCTTTTGAAGC+TGG	+	chr4.3:33554544-33554563	None:intergenic	50.0%
!!!	CCACAGCTTTTGAAGCTGGT+CGG	+	chr4.3:33554540-33554559	None:intergenic	50.0%
	CCGACCAGCTTCAAAAGCTG+TGG	-	chr4.3:33554537-33554556	MS.gene50685:CDS	55.0%
	CTTCCGTGCAAGGAATGGTG+AGG	+	chr4.3:33554708-33554727	None:intergenic	55.0%
	TGGCCTCACCATTCCTTGCA+CGG	-	chr4.3:33554702-33554721	MS.gene50685:CDS	55.0%
	GGAATGGTGAGGCCACCCAT+TGG	+	chr4.3:33554697-33554716	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	33554487	33554768	33554487	ID=MS.gene50685
chr4.3	mRNA	33554487	33554768	33554487	ID=MS.gene50685.t1;Parent=MS.gene50685
chr4.3	exon	33554487	33554768	33554487	ID=MS.gene50685.t1.exon1;Parent=MS.gene50685.t1
chr4.3	CDS	33554487	33554768	33554487	ID=cds.MS.gene50685.t1;Parent=MS.gene50685.t1

Gene Sequence	>MS.gene50685 ATGGGTTTTCGTTTCCCTGGTATCATCAGAAAGGCATCATTTTCTGCAAACCGACCAGCTTCAAAAGCTGTGGACGTGCCAAAGGGATATCTTGCAGTATATGTTGGAGAGAAACAGACACGGTATGTGATCCCAGTATCATACTTGAGCCAACCTTCATTTCAAGATTTGCTGAGTCAAGTTGAGGAAGAGTTTGGATATGATCATCCAATGGGTGGCCTCACCATTCCTTGCACGGAAGATGTCTTTCAACATATAACTTCTCGCTTCAATGGGCTATGA
Protein sequence	>MS.gene50685.t1 MGFRFPGIIRKASFSANRPASKAVDVPKGYLAVYVGEKQTRYVIPVSYLSQPSFQDLLSQVEEEFGYDHPMGGLTIPCTEDVFQHITSRFNGL