AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50686

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50686.t1 MTR_4g072870 87.912 91 11 0 1 91 1 91 1.35e-57 172
MS.gene50686.t1 MTR_4g072260 87.097 93 12 0 1 93 1 93 3.46e-57 171
MS.gene50686.t1 MTR_4g072220 86.022 93 13 0 1 93 1 93 9.32e-56 167
MS.gene50686.t1 MTR_4g072890 84.615 91 14 0 1 91 1 91 2.05e-54 164
MS.gene50686.t1 MTR_4g072240 80.220 91 18 0 1 91 1 91 2.92e-53 161
MS.gene50686.t1 MTR_4g072300 79.121 91 19 0 1 91 1 91 6.96e-52 157
MS.gene50686.t1 MTR_4g072370 73.626 91 21 1 1 91 1 88 2.58e-46 143
MS.gene50686.t1 MTR_4g072310 67.742 93 30 0 1 93 1 93 1.05e-45 142
MS.gene50686.t1 MTR_3g110038 72.043 93 25 1 1 93 1 92 2.43e-45 141
MS.gene50686.t1 MTR_4g072880 70.330 91 27 0 1 91 1 91 4.83e-45 140
MS.gene50686.t1 MTR_4g072580 72.527 91 19 1 1 91 1 85 7.89e-45 139
MS.gene50686.t1 MTR_3g084180 67.742 93 29 1 1 93 1 92 1.21e-44 139
MS.gene50686.t1 MTR_3g084150 66.667 93 30 1 1 93 1 92 2.28e-43 136
MS.gene50686.t1 MTR_3g084240 67.742 93 29 1 1 93 1 92 2.57e-43 136
MS.gene50686.t1 MTR_4g072330 68.132 91 26 1 1 91 1 88 4.43e-43 135
MS.gene50686.t1 MTR_4g072830 70.330 91 24 1 1 91 1 88 7.26e-43 135
MS.gene50686.t1 MTR_4g072500 65.591 93 31 1 1 93 1 92 8.69e-43 134
MS.gene50686.t1 MTR_3g110032 66.667 93 31 0 1 93 1 93 9.16e-43 134
MS.gene50686.t1 MTR_3g084170 67.742 93 29 1 1 93 1 92 1.03e-42 134
MS.gene50686.t1 MTR_4g072820 73.333 90 18 2 1 90 1 84 2.33e-42 133
MS.gene50686.t1 MTR_3g117630 69.412 85 26 0 9 93 8 92 2.81e-42 133
MS.gene50686.t1 MTR_4g072270 65.591 93 32 0 1 93 1 93 5.12e-42 132
MS.gene50686.t1 MTR_8g461410 64.516 93 32 1 1 93 1 92 5.61e-42 132
MS.gene50686.t1 MTR_4g072230 66.667 93 29 1 1 93 1 91 5.95e-42 132
MS.gene50686.t1 MTR_4g072740 68.132 91 23 1 1 91 1 85 7.40e-42 132
MS.gene50686.t1 MTR_4g050700 65.591 93 32 0 1 93 1 93 2.16e-41 131
MS.gene50686.t1 MTR_2g012410 65.591 93 32 0 1 93 1 93 2.16e-41 131
MS.gene50686.t1 MTR_4g072470 68.132 91 23 1 1 91 1 85 2.17e-41 130
MS.gene50686.t1 MTR_4g072640 65.217 92 31 1 1 92 1 91 2.64e-41 130
MS.gene50686.t1 MTR_4g072530 64.835 91 31 1 1 91 1 90 4.21e-41 130
MS.gene50686.t1 MTR_4g072250 65.934 91 30 1 1 91 1 90 5.74e-41 130
MS.gene50686.t1 MTR_3g084260 65.934 91 31 0 1 91 1 91 5.85e-41 130
MS.gene50686.t1 MTR_4g072650 71.429 91 19 2 1 91 1 84 7.71e-41 129
MS.gene50686.t1 MTR_3g084250 66.667 93 30 1 1 93 1 92 1.15e-40 129
MS.gene50686.t1 MTR_4g072610 67.033 91 23 1 1 91 1 84 1.29e-40 129
MS.gene50686.t1 MTR_3g084220 64.516 93 32 1 1 93 1 92 2.61e-40 128
MS.gene50686.t1 MTR_4g072860 63.441 93 34 0 1 93 1 93 4.50e-40 127
MS.gene50686.t1 MTR_3g110042 66.667 90 29 1 1 90 1 89 6.85e-40 127
MS.gene50686.t1 MTR_4g072570 63.441 93 33 1 1 93 1 92 7.23e-40 127
MS.gene50686.t1 MTR_4g072600 67.033 91 24 1 1 91 1 85 7.95e-40 127
MS.gene50686.t1 MTR_4g072630 65.934 91 26 1 1 91 1 86 8.52e-40 127
MS.gene50686.t1 MTR_4g072490 65.934 91 25 1 1 91 1 85 1.04e-39 126
MS.gene50686.t1 MTR_4g072480 62.366 93 34 1 1 93 1 92 3.40e-39 125
MS.gene50686.t1 MTR_4g072850 69.149 94 28 1 1 93 1 94 5.40e-39 125
MS.gene50686.t1 MTR_4g072730 61.538 91 34 1 1 91 1 90 6.70e-39 125
MS.gene50686.t1 MTR_4g072460 61.290 93 35 1 1 93 1 92 9.21e-39 124
MS.gene50686.t1 MTR_4g072680 60.215 93 36 1 1 93 1 92 6.14e-38 122
MS.gene50686.t1 MTR_4g072620 60.215 93 36 1 1 93 1 92 8.63e-38 122
MS.gene50686.t1 MTR_4g072540 75.714 70 17 0 22 91 17 86 2.32e-37 120
MS.gene50686.t1 MTR_3g084190 68.817 93 28 1 1 93 1 92 2.47e-37 120
MS.gene50686.t1 MTR_4g072280 74.667 75 19 0 1 75 1 75 1.15e-36 119
MS.gene50686.t1 MTR_8g461420 66.667 93 30 1 1 93 1 92 1.61e-36 119
MS.gene50686.t1 MTR_3g110045 67.033 91 29 1 3 93 62 151 3.62e-36 120
MS.gene50686.t1 MTR_4g072670 72.857 70 19 0 22 91 17 86 4.01e-36 117
MS.gene50686.t1 MTR_4g072380 64.773 88 26 1 1 88 1 83 4.08e-36 117
MS.gene50686.t1 MTR_3g084160 65.591 93 31 1 1 93 1 92 5.38e-36 117
MS.gene50686.t1 MTR_4g072510 74.286 70 18 0 22 91 17 86 5.76e-36 117
MS.gene50686.t1 MTR_3g084230 65.591 93 31 1 1 93 1 92 8.25e-36 117
MS.gene50686.t1 MTR_4g072940 72.222 72 20 0 22 93 53 124 1.66e-35 117
MS.gene50686.t1 MTR_4g072720 72.857 70 19 0 22 91 17 86 2.03e-35 115
MS.gene50686.t1 MTR_3g084210 65.591 93 31 1 1 93 1 92 2.19e-35 116
MS.gene50686.t1 MTR_4g072660 59.140 93 32 1 1 93 1 87 2.58e-35 115
MS.gene50686.t1 MTR_4g072465 59.783 92 30 2 1 91 1 86 4.73e-35 115
MS.gene50686.t1 MTR_4g072590 73.134 67 18 0 25 91 19 85 3.94e-34 112
MS.gene50686.t1 MTR_4g072690 75.758 66 16 0 26 91 20 85 8.30e-34 112
MS.gene50686.t1 MTR_5g008370 64.634 82 25 1 10 91 13 90 1.12e-33 113
MS.gene50686.t1 MTR_5g008360 64.474 76 27 0 18 93 8 83 1.75e-33 110
MS.gene50686.t1 MTR_5g008350 66.250 80 21 1 1 80 1 74 2.66e-33 110
MS.gene50686.t1 MTR_4g072550 65.753 73 25 0 21 93 31 103 3.61e-32 108
MS.gene50686.t1 MTR_3g084200 67.123 73 23 1 1 73 1 72 8.26e-32 107
MS.gene50686.t1 MTR_4g072450 71.212 66 19 0 26 91 20 85 3.16e-31 105
MS.gene50686.t1 MTR_4g072910 67.164 67 22 0 25 91 34 100 3.67e-31 105
MS.gene50686.t1 MTR_4g072770 66.667 66 22 0 26 91 15 80 2.73e-30 102
MS.gene50686.t1 MTR_4g072560 65.672 67 22 1 25 90 31 97 4.85e-30 102
MS.gene50686.t1 MTR_3g117640 65.672 67 22 1 25 90 33 99 5.84e-30 102
MS.gene50686.t1 MTR_4g072980 57.895 76 31 1 16 90 17 92 6.78e-30 102
MS.gene50686.t1 MTR_4g072750 71.212 66 17 1 10 73 9 74 4.14e-29 100
MS.gene50686.t1 MTR_4g072290 53.763 93 29 1 1 93 1 79 5.55e-29 99.8
MS.gene50686.t1 MTR_4g072930 51.765 85 37 1 11 91 26 110 1.83e-27 96.7
MS.gene50686.t1 MTR_3g110035 65.152 66 23 0 28 93 73 138 1.96e-25 92.4
MS.gene50686.t1 MTR_3g113310 48.913 92 40 3 5 90 15 105 6.61e-25 90.1
MS.gene50686.t1 MTR_3g117610 58.462 65 26 1 24 88 43 106 2.01e-23 86.3
MS.gene50686.t1 MTR_4g072190 46.512 86 43 2 5 90 17 99 1.61e-22 84.0
MS.gene50686.t1 MTR_6g077850 53.731 67 31 0 25 91 33 99 1.96e-22 83.6
MS.gene50686.t1 MTR_8g076040 48.864 88 40 3 5 88 16 102 5.73e-22 82.4
MS.gene50686.t1 MTR_5g021820 47.778 90 42 4 5 90 15 103 5.83e-22 82.4
MS.gene50686.t1 MTR_8g461400 46.237 93 23 2 1 93 1 66 2.76e-20 77.0
MS.gene50686.t1 MTR_4g072840 51.111 90 13 3 1 90 1 59 2.34e-19 74.7
MS.gene50686.t1 MTR_2g043960 59.322 59 23 1 25 83 75 132 2.67e-18 74.7
MS.gene50686.t1 MTR_4g124750 56.250 64 27 1 25 88 68 130 2.75e-18 74.7
MS.gene50686.t1 MTR_4g005310 50.725 69 33 1 19 86 55 123 4.46e-18 73.9
MS.gene50686.t1 MTR_4g025830 45.833 72 35 2 23 91 76 146 6.21e-16 68.6
MS.gene50686.t1 MTR_1g094750 39.535 86 47 2 11 92 31 115 7.48e-16 67.4
MS.gene50686.t1 MTR_1g009150 49.254 67 33 1 17 83 62 127 8.11e-16 68.6
MS.gene50686.t1 MTR_0388s0020 37.931 87 49 3 8 91 58 142 8.47e-16 68.2
MS.gene50686.t1 MTR_4g072160 43.662 71 39 1 16 86 27 96 4.17e-15 65.9
MS.gene50686.t1 MTR_3g109160 45.570 79 42 1 5 83 48 125 4.42e-15 66.6
MS.gene50686.t1 MTR_3g092220 42.353 85 42 4 1 83 12 91 7.42e-15 64.7
MS.gene50686.t1 MTR_1g024085 50.847 59 28 1 25 83 36 93 2.51e-14 64.3
MS.gene50686.t1 MTR_7g118010 47.619 63 30 2 26 86 23 84 3.11e-14 62.8
MS.gene50686.t1 MTR_4g005320 50.000 58 28 1 26 83 70 126 3.25e-14 64.3
MS.gene50686.t1 MTR_1g061570 51.786 56 26 1 25 80 18 72 1.03e-13 62.0
MS.gene50686.t1 MTR_3g097120 41.772 79 37 2 22 92 5 82 1.24e-13 61.2
MS.gene50686.t1 MTR_7g101740 45.312 64 34 1 25 88 39 101 2.08e-13 60.8
MS.gene50686.t1 MTR_8g026730 46.667 60 29 2 26 83 27 85 3.29e-13 60.5
MS.gene50686.t1 MTR_1g063950 41.935 62 35 1 25 86 47 107 5.30e-13 60.1
MS.gene50686.t1 MTR_8g461210 45.763 59 31 1 25 83 34 91 1.11e-12 59.3
MS.gene50686.t1 MTR_7g104950 38.158 76 41 2 15 90 44 113 3.71e-12 58.2
MS.gene50686.t1 MTR_1g059770 39.062 64 38 1 27 90 50 112 5.77e-12 57.8
MS.gene50686.t1 MTR_7g405740 45.000 60 32 1 27 86 68 126 9.61e-12 57.8
MS.gene50686.t1 MTR_3g105900 63.889 36 13 0 25 60 1171 1206 1.42e-11 59.3
MS.gene50686.t1 MTR_2g066830 43.396 53 29 1 26 78 58 109 1.97e-11 56.6
MS.gene50686.t1 MTR_7g104940 36.842 76 42 2 15 90 44 113 3.42e-11 55.8
MS.gene50686.t1 MTR_8g096440 39.241 79 45 2 7 85 28 103 3.58e-11 55.8
MS.gene50686.t1 MTR_4g021810 44.118 68 35 3 17 83 18 83 3.86e-11 55.1
MS.gene50686.t1 MTR_3g098970 33.333 69 45 1 25 93 52 119 5.20e-11 55.5
MS.gene50686.t1 MTR_8g094980 38.272 81 36 2 25 92 2 81 5.64e-11 54.7
MS.gene50686.t1 MTR_8g101520 42.424 66 37 1 27 92 51 115 9.31e-11 55.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50686.t1 AT4G38840 61.176 85 32 1 8 91 15 99 1.30e-33 112
MS.gene50686.t1 AT2G21210 67.568 74 22 1 20 91 22 95 1.29e-32 109
MS.gene50686.t1 AT2G21210 67.568 74 22 1 20 91 89 162 5.46e-32 109
MS.gene50686.t1 AT5G18080 61.538 78 27 2 14 90 14 89 9.78e-31 104
MS.gene50686.t1 AT5G18030 69.231 65 19 1 27 90 23 87 1.30e-30 103
MS.gene50686.t1 AT5G18020 58.667 75 30 1 17 90 15 89 2.03e-30 103
MS.gene50686.t1 AT4G38825 60.811 74 28 1 18 90 15 88 3.24e-30 103
MS.gene50686.t1 AT5G18060 67.692 65 20 1 27 90 25 89 3.37e-30 103
MS.gene50686.t1 AT5G18010 60.000 75 29 1 17 90 15 89 4.38e-30 102
MS.gene50686.t1 AT5G18050 66.154 65 21 1 27 90 25 89 8.19e-30 102
MS.gene50686.t1 AT2G21200 61.111 72 27 1 20 90 15 86 3.82e-29 100
MS.gene50686.t1 AT4G34770 60.714 84 28 2 10 91 22 102 4.34e-29 100
MS.gene50686.t1 AT3G03840 62.162 74 26 1 19 90 20 93 7.81e-29 99.8
MS.gene50686.t1 AT3G03820 55.435 92 35 2 2 90 6 94 6.08e-28 97.4
MS.gene50686.t1 AT4G38850 54.651 86 26 2 8 90 13 88 9.54e-28 96.7
MS.gene50686.t1 AT3G03850 63.636 66 22 1 27 90 26 91 1.22e-26 94.0
MS.gene50686.t1 AT4G34810 60.000 70 23 2 26 90 48 117 1.39e-26 94.7
MS.gene50686.t1 AT3G03830 62.687 67 22 1 27 90 25 91 2.35e-26 93.2
MS.gene50686.t1 AT4G34800 49.438 89 37 2 1 86 1 84 2.48e-26 93.2
MS.gene50686.t1 AT4G13790 46.667 90 40 2 3 90 8 91 2.69e-25 90.5
MS.gene50686.t1 AT4G34790 60.000 65 24 1 26 88 39 103 1.18e-24 89.4
MS.gene50686.t1 AT4G38860 55.224 67 30 0 24 90 38 104 1.00e-23 87.0
MS.gene50686.t1 AT1G75580 48.936 94 39 3 5 90 15 107 1.34e-23 87.0
MS.gene50686.t1 AT4G34780 57.143 70 27 2 25 91 27 96 8.37e-23 84.7
MS.gene50686.t1 AT2G21220 46.429 84 41 2 10 90 21 103 5.98e-22 82.4
MS.gene50686.t1 AT4G34760 53.731 67 30 1 24 90 41 106 7.97e-22 82.4
MS.gene50686.t1 AT1G19830 52.778 72 33 1 19 90 41 111 1.93e-21 81.6
MS.gene50686.t1 AT4G36110 48.193 83 40 2 8 88 20 101 1.56e-20 79.0
MS.gene50686.t1 AT2G16580 45.455 88 40 2 8 88 19 105 3.11e-19 75.9
MS.gene50686.t1 AT3G43120 57.627 59 24 1 25 83 78 135 6.89e-19 76.3
MS.gene50686.t1 AT2G18010 41.489 94 44 2 5 88 17 109 1.65e-18 73.9
MS.gene50686.t1 AT5G20810 54.545 66 29 1 18 83 71 135 5.12e-18 74.3
MS.gene50686.t1 AT5G20810 54.545 66 29 1 18 83 71 135 1.02e-17 73.9
MS.gene50686.t1 AT5G66260 45.783 83 43 2 10 91 17 98 7.44e-17 69.3
MS.gene50686.t1 AT3G20220 50.769 65 30 2 26 89 47 110 1.02e-16 69.7
MS.gene50686.t1 AT3G51200 49.206 63 30 2 25 86 38 99 2.78e-15 65.5
MS.gene50686.t1 AT1G75590 47.222 72 33 2 12 83 32 98 5.38e-15 66.2
MS.gene50686.t1 AT2G37030 44.444 63 34 1 25 87 48 109 2.95e-14 63.5
MS.gene50686.t1 AT5G10990 49.153 59 29 1 25 83 40 97 7.58e-14 63.2
MS.gene50686.t1 AT5G53590 41.096 73 37 2 25 92 45 116 1.06e-13 62.4
MS.gene50686.t1 AT2G45210 39.726 73 40 2 22 91 75 146 1.47e-13 62.4
MS.gene50686.t1 AT1G19840 50.847 59 28 1 25 83 40 97 1.66e-13 62.4
MS.gene50686.t1 AT3G53250 42.857 63 32 1 25 87 51 109 2.50e-13 61.2
MS.gene50686.t1 AT4G31320 49.231 65 32 1 14 78 68 131 2.76e-13 62.4
MS.gene50686.t1 AT3G53250 42.857 63 32 1 25 87 82 140 3.31e-13 61.6
MS.gene50686.t1 AT3G60690 42.647 68 35 2 26 90 88 154 3.47e-13 61.6
MS.gene50686.t1 AT4G12410 44.928 69 34 2 26 91 76 143 4.21e-13 61.2
MS.gene50686.t1 AT2G46690 43.284 67 34 2 20 83 18 83 7.59e-13 59.7
MS.gene50686.t1 AT4G09530 37.079 89 48 3 1 86 8 91 9.26e-13 59.3
MS.gene50686.t1 AT4G34750 44.068 59 32 1 25 83 36 93 1.74e-12 59.7
MS.gene50686.t1 AT4G34750 44.068 59 32 1 25 83 36 93 1.74e-12 59.7
MS.gene50686.t1 AT2G24400 51.724 58 27 1 26 83 64 120 1.75e-12 60.1
MS.gene50686.t1 AT4G00880 50.820 61 27 2 25 83 27 86 2.23e-12 58.5
MS.gene50686.t1 AT3G61900 45.161 62 30 2 25 83 29 89 3.02e-12 58.5
MS.gene50686.t1 AT3G12830 41.558 77 37 3 2 78 33 101 5.84e-12 57.8
MS.gene50686.t1 AT4G22620 40.580 69 37 2 26 91 79 146 1.54e-11 57.4
MS.gene50686.t1 AT1G56150 39.683 63 37 1 16 78 28 89 6.30e-11 54.7
MS.gene50686.t1 AT3G03847 52.830 53 23 2 34 86 80 130 6.59e-11 55.1

Find 23 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCGTAGATCCTTGAGTTT+TGG 0.251706 4.3:-33555863 None:intergenic
AATCAAGCAGAAGAAGAATT+TGG 0.271748 4.3:+33555994 MS.gene50686:CDS
TAGACTGCAAGATAGCCTTT+TGG 0.386300 4.3:-33555898 None:intergenic
GGCTGGCTCAAATATGATAC+TGG 0.405573 4.3:-33555952 None:intergenic
GGCTATCTTGCAGTCTATGT+TGG 0.480274 4.3:+33555904 MS.gene50686:CDS
TGATGCCCTTCTGATAATAC+CGG 0.494694 4.3:-33555836 None:intergenic
GTAATTCTTGAAACGATGGC+TGG 0.519820 4.3:-33555969 None:intergenic
AAGGGATTTGAAATCCCAAA+AGG 0.535913 4.3:+33555883 MS.gene50686:CDS
TTTGGATATGATCATCCAAT+GGG 0.541874 4.3:+33556012 MS.gene50686:CDS
TTCTGATAATACCGGCTATG+TGG 0.555038 4.3:-33555828 None:intergenic
ATTTGGATATGATCATCCAA+TGG 0.556875 4.3:+33556011 MS.gene50686:CDS
ACATAGCCGGTATTATCAGA+AGG 0.569655 4.3:+33555830 MS.gene50686:CDS
TTTAGTAATTCTTGAAACGA+TGG 0.570741 4.3:-33555973 None:intergenic
AGAATGGGTTTCCACATAGC+CGG 0.572987 4.3:+33555817 None:intergenic
GGAATTGTAAGACCACCCAT+TGG 0.603707 4.3:-33556027 None:intergenic
CATAGCCGGTATTATCAGAA+GGG 0.608292 4.3:+33555831 MS.gene50686:CDS
CAAAACTCAAGGATCTACGA+AGG 0.612377 4.3:+33555864 MS.gene50686:CDS
TCTATGTTGGAGATAGAATG+AGG 0.630157 4.3:+33555917 MS.gene50686:CDS
AAGAAAGCATCTTCACCGCA+AGG 0.631158 4.3:-33556048 None:intergenic
AAAACTCAAGGATCTACGAA+GGG 0.636297 4.3:+33555865 MS.gene50686:CDS
GGATATGATCATCCAATGGG+TGG 0.655640 4.3:+33556015 MS.gene50686:CDS
GGTGGTCTTACAATTCCTTG+CGG 0.688498 4.3:+33556033 MS.gene50686:CDS
ATGTTGGAGATAGAATGAGG+CGG 0.759369 4.3:+33555920 MS.gene50686:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TTTAGTAATTCTTGAAACGA+TGG - chr4.3:33555976-33555995 None:intergenic 25.0%
AATCAAGCAGAAGAAGAATT+TGG + chr4.3:33555994-33556013 MS.gene50686:CDS 30.0%
ATTTGGATATGATCATCCAA+TGG + chr4.3:33556011-33556030 MS.gene50686:CDS 30.0%
TTTGGATATGATCATCCAAT+GGG + chr4.3:33556012-33556031 MS.gene50686:CDS 30.0%
AAAACTCAAGGATCTACGAA+GGG + chr4.3:33555865-33555884 MS.gene50686:CDS 35.0%
TCTATGTTGGAGATAGAATG+AGG + chr4.3:33555917-33555936 MS.gene50686:CDS 35.0%
! AAGGGATTTGAAATCCCAAA+AGG + chr4.3:33555883-33555902 MS.gene50686:CDS 35.0%
! GCATCATTTTCCAAAACTCA+AGG + chr4.3:33555853-33555872 MS.gene50686:CDS 35.0%
ACATAGCCGGTATTATCAGA+AGG + chr4.3:33555830-33555849 MS.gene50686:CDS 40.0%
ATGTTGGAGATAGAATGAGG+CGG + chr4.3:33555920-33555939 MS.gene50686:CDS 40.0%
CAAAACTCAAGGATCTACGA+AGG + chr4.3:33555864-33555883 MS.gene50686:CDS 40.0%
CATAGCCGGTATTATCAGAA+GGG + chr4.3:33555831-33555850 MS.gene50686:CDS 40.0%
GTAATTCTTGAAACGATGGC+TGG - chr4.3:33555972-33555991 None:intergenic 40.0%
TGATGCCCTTCTGATAATAC+CGG - chr4.3:33555839-33555858 None:intergenic 40.0%
! AGACTGCAAGATAGCCTTTT+GGG - chr4.3:33555900-33555919 None:intergenic 40.0%
! CTTCGTAGATCCTTGAGTTT+TGG - chr4.3:33555866-33555885 None:intergenic 40.0%
! TAGACTGCAAGATAGCCTTT+TGG - chr4.3:33555901-33555920 None:intergenic 40.0%
! TTCTGATAATACCGGCTATG+TGG - chr4.3:33555831-33555850 None:intergenic 40.0%
GGAATTGTAAGACCACCCAT+TGG - chr4.3:33556030-33556049 None:intergenic 45.0%
GGATATGATCATCCAATGGG+TGG + chr4.3:33556015-33556034 MS.gene50686:CDS 45.0%
GGCTATCTTGCAGTCTATGT+TGG + chr4.3:33555904-33555923 MS.gene50686:CDS 45.0%
! GGCTGGCTCAAATATGATAC+TGG - chr4.3:33555955-33555974 None:intergenic 45.0%
! GGTGGTCTTACAATTCCTTG+CGG + chr4.3:33556033-33556052 MS.gene50686:CDS 45.0%
!! AAGAAAGCATCTTCACCGCA+AGG - chr4.3:33556051-33556070 None:intergenic 45.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 33555820 33556101 33555820 ID=MS.gene50686
chr4.3 mRNA 33555820 33556101 33555820 ID=MS.gene50686.t1;Parent=MS.gene50686
chr4.3 exon 33555820 33556101 33555820 ID=MS.gene50686.t1.exon1;Parent=MS.gene50686.t1
chr4.3 CDS 33555820 33556101 33555820 ID=cds.MS.gene50686.t1;Parent=MS.gene50686.t1
Gene Sequence

>MS.gene50686

ATGGGTTTCCACATAGCCGGTATTATCAGAAGGGCATCATTTTCCAAAACTCAAGGATCTACGAAGGGATTTGAAATCCCAAAAGGCTATCTTGCAGTCTATGTTGGAGATAGAATGAGGCGGTTCGTGATTCCAGTATCATATTTGAGCCAGCCATCGTTTCAAGAATTACTAAATCAAGCAGAAGAAGAATTTGGATATGATCATCCAATGGGTGGTCTTACAATTCCTTGCGGTGAAGATGCTTTCTTACAACTCACTTCTCGCTTAAGTGAGTTGTAA
Protein sequence

>MS.gene50686.t1

MGFHIAGIIRRASFSKTQGSTKGFEIPKGYLAVYVGDRMRRFVIPVSYLSQPSFQELLNQAEEEFGYDHPMGGLTIPCGEDAFLQLTSRLSEL