Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53366.t1 | AFK43109.1 | 89.8 | 98 | 7 | 1 | 1 | 95 | 1 | 98 | 4.80E-41 | 176.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53366.t1 | I3SS67 | 89.8 | 98 | 7 | 1 | 1 | 95 | 1 | 98 | 3.5e-41 | 176.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene53366 | -0.823538 | 1.31E-53 | -1.69E-46 |
| MS.gene049217 | MS.gene53366 | -0.808902 | 2.46E-50 | -1.69E-46 |
| MS.gene049222 | MS.gene53366 | -0.843462 | 1.38E-58 | -1.69E-46 |
| MS.gene049285 | MS.gene53366 | -0.803657 | 3.14E-49 | -1.69E-46 |
| MS.gene049298 | MS.gene53366 | 0.816922 | 4.31E-52 | -1.69E-46 |
| MS.gene049320 | MS.gene53366 | -0.801594 | 8.37E-49 | -1.69E-46 |
| MS.gene04939 | MS.gene53366 | -0.801147 | 1.03E-48 | -1.69E-46 |
| MS.gene049633 | MS.gene53366 | -0.858581 | 7.47E-63 | -1.69E-46 |
| MS.gene050165 | MS.gene53366 | -0.805535 | 1.27E-49 | -1.69E-46 |
| MS.gene050170 | MS.gene53366 | -0.830467 | 2.89E-55 | -1.69E-46 |
| MS.gene050323 | MS.gene53366 | -0.831006 | 2.13E-55 | -1.69E-46 |
| MS.gene050338 | MS.gene53366 | 0.853088 | 3.01E-61 | -1.69E-46 |
| MS.gene050444 | MS.gene53366 | -0.865838 | 4.41E-65 | -1.69E-46 |
| MS.gene050504 | MS.gene53366 | -0.800405 | 1.46E-48 | -1.69E-46 |
| MS.gene050577 | MS.gene53366 | -0.829055 | 6.38E-55 | -1.69E-46 |
| MS.gene050612 | MS.gene53366 | -0.814195 | 1.74E-51 | -1.69E-46 |
| MS.gene050710 | MS.gene53366 | 0.818322 | 2.08E-52 | -1.69E-46 |
| MS.gene050747 | MS.gene53366 | -0.810063 | 1.39E-50 | -1.69E-46 |
| MS.gene05080 | MS.gene53366 | -0.848888 | 4.60E-60 | -1.69E-46 |
| MS.gene05081 | MS.gene53366 | -0.820016 | 8.57E-53 | -1.69E-46 |
| MS.gene050868 | MS.gene53366 | -0.827551 | 1.47E-54 | -1.69E-46 |
| MS.gene050871 | MS.gene53366 | -0.826575 | 2.52E-54 | -1.69E-46 |
| MS.gene050904 | MS.gene53366 | -0.861704 | 8.51E-64 | -1.69E-46 |
| MS.gene051317 | MS.gene53366 | -0.844283 | 8.32E-59 | -1.69E-46 |
| MS.gene051651 | MS.gene53366 | 0.821604 | 3.70E-53 | -1.69E-46 |
| MS.gene051838 | MS.gene53366 | -0.871381 | 7.14E-67 | -1.69E-46 |
| MS.gene052129 | MS.gene53366 | 0.820102 | 8.19E-53 | -1.69E-46 |
| MS.gene052164 | MS.gene53366 | -0.839255 | 1.77E-57 | -1.69E-46 |
| MS.gene052503 | MS.gene53366 | -0.822662 | 2.10E-53 | -1.69E-46 |
| MS.gene052823 | MS.gene53366 | -0.81066 | 1.03E-50 | -1.69E-46 |
| MS.gene052825 | MS.gene53366 | -0.817329 | 3.49E-52 | -1.69E-46 |
| MS.gene052870 | MS.gene53366 | -0.811822 | 5.78E-51 | -1.69E-46 |
| MS.gene052872 | MS.gene53366 | -0.830836 | 2.35E-55 | -1.69E-46 |
| MS.gene052966 | MS.gene53366 | -0.817413 | 3.34E-52 | -1.69E-46 |
| MS.gene053108 | MS.gene53366 | 0.825662 | 4.15E-54 | -1.69E-46 |
| MS.gene053203 | MS.gene53366 | -0.802887 | 4.53E-49 | -1.69E-46 |
| MS.gene053479 | MS.gene53366 | -0.810711 | 1.01E-50 | -1.69E-46 |
| MS.gene053480 | MS.gene53366 | -0.823562 | 1.30E-53 | -1.69E-46 |
| MS.gene053632 | MS.gene53366 | -0.836546 | 8.78E-57 | -1.69E-46 |
| MS.gene053824 | MS.gene53366 | -0.832613 | 8.55E-56 | -1.69E-46 |
| MS.gene053916 | MS.gene53366 | -0.812282 | 4.59E-51 | -1.69E-46 |
| MS.gene054600 | MS.gene53366 | -0.801769 | 7.70E-49 | -1.69E-46 |
| MS.gene054706 | MS.gene53366 | 0.824501 | 7.80E-54 | -1.69E-46 |
| MS.gene05470 | MS.gene53366 | -0.837281 | 5.70E-57 | -1.69E-46 |
| MS.gene054850 | MS.gene53366 | 0.804693 | 1.91E-49 | -1.69E-46 |
| MS.gene054884 | MS.gene53366 | 0.809768 | 1.61E-50 | -1.69E-46 |
| MS.gene054941 | MS.gene53366 | 0.836423 | 9.44E-57 | -1.69E-46 |
| MS.gene05494 | MS.gene53366 | -0.820605 | 6.28E-53 | -1.69E-46 |
| MS.gene055206 | MS.gene53366 | -0.803245 | 3.82E-49 | -1.69E-46 |
| MS.gene055502 | MS.gene53366 | -0.819841 | 9.40E-53 | -1.69E-46 |
| MS.gene055539 | MS.gene53366 | -0.801836 | 7.46E-49 | -1.69E-46 |
| MS.gene055577 | MS.gene53366 | -0.83396 | 3.95E-56 | -1.69E-46 |
| MS.gene055729 | MS.gene53366 | 0.807845 | 4.14E-50 | -1.69E-46 |
| MS.gene055730 | MS.gene53366 | 0.802782 | 4.77E-49 | -1.69E-46 |
| MS.gene055731 | MS.gene53366 | 0.825296 | 5.06E-54 | -1.69E-46 |
| MS.gene055748 | MS.gene53366 | -0.863581 | 2.25E-64 | -1.69E-46 |
| MS.gene055750 | MS.gene53366 | -0.844196 | 8.78E-59 | -1.69E-46 |
| MS.gene055751 | MS.gene53366 | -0.860488 | 1.99E-63 | -1.69E-46 |
| MS.gene05589 | MS.gene53366 | -0.870986 | 9.64E-67 | -1.69E-46 |
| MS.gene055941 | MS.gene53366 | -0.827566 | 1.46E-54 | -1.69E-46 |
| MS.gene056283 | MS.gene53366 | -0.861377 | 1.07E-63 | -1.69E-46 |
| MS.gene056387 | MS.gene53366 | -0.87782 | 4.62E-69 | -1.69E-46 |
| MS.gene056487 | MS.gene53366 | -0.806249 | 9.02E-50 | -1.69E-46 |
| MS.gene056504 | MS.gene53366 | -0.841466 | 4.67E-58 | -1.69E-46 |
| MS.gene056510 | MS.gene53366 | -0.807687 | 4.48E-50 | -1.69E-46 |
| MS.gene056618 | MS.gene53366 | 0.871714 | 5.54E-67 | -1.69E-46 |
| MS.gene05664 | MS.gene53366 | -0.802973 | 4.35E-49 | -1.69E-46 |
| MS.gene056657 | MS.gene53366 | -0.811667 | 6.24E-51 | -1.69E-46 |
| MS.gene05666 | MS.gene53366 | -0.802401 | 5.71E-49 | -1.69E-46 |
| MS.gene056678 | MS.gene53366 | 0.800109 | 1.68E-48 | -1.69E-46 |
| MS.gene056692 | MS.gene53366 | -0.804387 | 2.21E-49 | -1.69E-46 |
| MS.gene056697 | MS.gene53366 | 0.804364 | 2.24E-49 | -1.69E-46 |
| MS.gene057076 | MS.gene53366 | -0.817632 | 2.98E-52 | -1.69E-46 |
| MS.gene057658 | MS.gene53366 | -0.816365 | 5.74E-52 | -1.69E-46 |
| MS.gene057717 | MS.gene53366 | 0.817606 | 3.02E-52 | -1.69E-46 |
| MS.gene057734 | MS.gene53366 | -0.814325 | 1.63E-51 | -1.69E-46 |
| MS.gene057831 | MS.gene53366 | -0.831506 | 1.61E-55 | -1.69E-46 |
| MS.gene057857 | MS.gene53366 | -0.831468 | 1.64E-55 | -1.69E-46 |
| MS.gene058128 | MS.gene53366 | 0.809967 | 1.46E-50 | -1.69E-46 |
| MS.gene058334 | MS.gene53366 | 0.815204 | 1.04E-51 | -1.69E-46 |
| MS.gene058338 | MS.gene53366 | -0.826032 | 3.39E-54 | -1.69E-46 |
| MS.gene058419 | MS.gene53366 | -0.80691 | 6.54E-50 | -1.69E-46 |
| MS.gene058838 | MS.gene53366 | -0.849643 | 2.83E-60 | -1.69E-46 |
| MS.gene058853 | MS.gene53366 | 0.832189 | 1.09E-55 | -1.69E-46 |
| MS.gene059023 | MS.gene53366 | -0.835403 | 1.71E-56 | -1.69E-46 |
| MS.gene05914 | MS.gene53366 | -0.807281 | 5.46E-50 | -1.69E-46 |
| MS.gene05926 | MS.gene53366 | 0.846901 | 1.62E-59 | -1.69E-46 |
| MS.gene05934 | MS.gene53366 | -0.817541 | 3.13E-52 | -1.69E-46 |
| MS.gene059526 | MS.gene53366 | 0.815085 | 1.11E-51 | -1.69E-46 |
| MS.gene05960 | MS.gene53366 | 0.832468 | 9.29E-56 | -1.69E-46 |
| MS.gene059667 | MS.gene53366 | -0.834988 | 2.18E-56 | -1.69E-46 |
| MS.gene059677 | MS.gene53366 | -0.810769 | 9.78E-51 | -1.69E-46 |
| MS.gene059699 | MS.gene53366 | 0.804156 | 2.47E-49 | -1.69E-46 |
| MS.gene059703 | MS.gene53366 | 0.800321 | 1.52E-48 | -1.69E-46 |
| MS.gene059717 | MS.gene53366 | -0.805502 | 1.29E-49 | -1.69E-46 |
| MS.gene059718 | MS.gene53366 | -0.809615 | 1.73E-50 | -1.69E-46 |
| MS.gene059865 | MS.gene53366 | -0.822496 | 2.30E-53 | -1.69E-46 |
| MS.gene06000 | MS.gene53366 | -0.835632 | 1.50E-56 | -1.69E-46 |
| MS.gene060405 | MS.gene53366 | 0.821414 | 4.09E-53 | -1.69E-46 |
| MS.gene060762 | MS.gene53366 | -0.857791 | 1.28E-62 | -1.69E-46 |
| MS.gene060778 | MS.gene53366 | -0.811595 | 6.47E-51 | -1.69E-46 |
| MS.gene060823 | MS.gene53366 | 0.802614 | 5.16E-49 | -1.69E-46 |
| MS.gene060982 | MS.gene53366 | -0.856342 | 3.43E-62 | -1.69E-46 |
| MS.gene061044 | MS.gene53366 | 0.814331 | 1.63E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene53366.t1 | MTR_8g087660 | 97.222 | 72 | 2 | 0 | 24 | 95 | 1 | 72 | 4.73e-47 | 145 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTCTAAATCTGAAGCTTTA+AGG | 0.110020 | 4.1:-13125422 | MS.gene53366:CDS |
| CTCTCTCCTTGTTTGTCAAA+TGG | 0.324643 | 4.1:-13125595 | MS.gene53366:CDS |
| AAGAAGAGTAGCTTGGAGTT+GGG | 0.345283 | 4.1:+13125630 | None:intergenic |
| AGCTTTAAGGTTGCCTCACC+AGG | 0.416451 | 4.1:-13125409 | MS.gene53366:CDS |
| GAAGAAGAGTAGCTTGGAGT+TGG | 0.423365 | 4.1:+13125629 | None:intergenic |
| CCAACTCTCCACAGTTGATA+AGG | 0.434883 | 4.1:+13125517 | None:intergenic |
| CTCAGAACTCCAAGTGCAGC+TGG | 0.436184 | 4.1:-13125476 | MS.gene53366:CDS |
| GATGATGAAGAAGAGTAGCT+TGG | 0.451530 | 4.1:+13125623 | None:intergenic |
| ACTGGTGCACCAGCTGCACT+TGG | 0.490099 | 4.1:+13125467 | None:intergenic |
| AAAAGATAGATCTCATAAAC+GGG | 0.490121 | 4.1:+13125368 | None:intergenic |
| CCTTGTTTGTCAAATGGTGG+TGG | 0.499531 | 4.1:-13125589 | MS.gene53366:CDS |
| AGAAGAGTAGCTTGGAGTTG+GGG | 0.506919 | 4.1:+13125631 | None:intergenic |
| CTCTCCACAGTTGATAAGGA+TGG | 0.512074 | 4.1:+13125521 | None:intergenic |
| CCTTATCAACTGTGGAGAGT+TGG | 0.513468 | 4.1:-13125517 | MS.gene53366:CDS |
| CAAATGGTGGTGGCTGAAGA+TGG | 0.538267 | 4.1:-13125579 | MS.gene53366:CDS |
| TCTCCTTGTTTGTCAAATGG+TGG | 0.559866 | 4.1:-13125592 | MS.gene53366:CDS |
| AGTATCATCAAGATCTTGAC+TGG | 0.560635 | 4.1:+13125449 | None:intergenic |
| TGGGCCCACAAGAGAAAACC+TGG | 0.594100 | 4.1:+13125391 | None:intergenic |
| TCTCCACAGTTGATAAGGAT+GGG | 0.596020 | 4.1:+13125522 | None:intergenic |
| CCACCACCATTTGACAAACA+AGG | 0.597993 | 4.1:+13125589 | None:intergenic |
| AGATAGATCTCATAAACGGG+TGG | 0.606925 | 4.1:+13125371 | None:intergenic |
| CCACAAGAGAAAACCTGGTG+AGG | 0.632813 | 4.1:+13125396 | None:intergenic |
| GATAGATCTCATAAACGGGT+GGG | 0.637322 | 4.1:+13125372 | None:intergenic |
| TGGCTGAAGATGGTTCATCA+AGG | 0.647563 | 4.1:-13125569 | MS.gene53366:CDS |
| AGACCCATCCTTATCAACTG+TGG | 0.653046 | 4.1:-13125525 | MS.gene53366:CDS |
| GTTGGGGATAGAGAACTCCA+TGG | 0.655236 | 4.1:+13125647 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TCTCTAAATCTGAAGCTTTA+AGG | - | chr4.1:13125603-13125622 | MS.gene53366:CDS | 30.0% |
| AGTATCATCAAGATCTTGAC+TGG | + | chr4.1:13125579-13125598 | None:intergenic | 35.0% | |
| AAGAAGAGTAGCTTGGAGTT+GGG | + | chr4.1:13125398-13125417 | None:intergenic | 40.0% | |
| GATGATGAAGAAGAGTAGCT+TGG | + | chr4.1:13125405-13125424 | None:intergenic | 40.0% | |
| ! | CTCTCTCCTTGTTTGTCAAA+TGG | - | chr4.1:13125430-13125449 | MS.gene53366:CDS | 40.0% |
| ! | TCTCCTTGTTTGTCAAATGG+TGG | - | chr4.1:13125433-13125452 | MS.gene53366:CDS | 40.0% |
| !! | TCTCCACAGTTGATAAGGAT+GGG | + | chr4.1:13125506-13125525 | None:intergenic | 40.0% |
| AGAAGAGTAGCTTGGAGTTG+GGG | + | chr4.1:13125397-13125416 | None:intergenic | 45.0% | |
| AGACCCATCCTTATCAACTG+TGG | - | chr4.1:13125500-13125519 | MS.gene53366:CDS | 45.0% | |
| CCACCACCATTTGACAAACA+AGG | + | chr4.1:13125439-13125458 | None:intergenic | 45.0% | |
| CCTTATCAACTGTGGAGAGT+TGG | - | chr4.1:13125508-13125527 | MS.gene53366:CDS | 45.0% | |
| GAAGAAGAGTAGCTTGGAGT+TGG | + | chr4.1:13125399-13125418 | None:intergenic | 45.0% | |
| TGGCTGAAGATGGTTCATCA+AGG | - | chr4.1:13125456-13125475 | MS.gene53366:CDS | 45.0% | |
| ! | CCAACTCTCCACAGTTGATA+AGG | + | chr4.1:13125511-13125530 | None:intergenic | 45.0% |
| ! | CCTTGTTTGTCAAATGGTGG+TGG | - | chr4.1:13125436-13125455 | MS.gene53366:CDS | 45.0% |
| ! | CTCACCAGGTTTTCTCTTGT+GGG | - | chr4.1:13125630-13125649 | MS.gene53366:CDS | 45.0% |
| ! | GATAAGGATGGGTCTTTTGC+TGG | + | chr4.1:13125495-13125514 | None:intergenic | 45.0% |
| !! | CTCTCCACAGTTGATAAGGA+TGG | + | chr4.1:13125507-13125526 | None:intergenic | 45.0% |
| !! | GGTCTTTTGCTGGCTGAAAA+GGG | + | chr4.1:13125485-13125504 | None:intergenic | 45.0% |
| CCACAAGAGAAAACCTGGTG+AGG | + | chr4.1:13125632-13125651 | None:intergenic | 50.0% | |
| ! | CCTCACCAGGTTTTCTCTTG+TGG | - | chr4.1:13125629-13125648 | MS.gene53366:CDS | 50.0% |
| !! | AGCTTTAAGGTTGCCTCACC+AGG | - | chr4.1:13125616-13125635 | MS.gene53366:CDS | 50.0% |
| !! | CAAATGGTGGTGGCTGAAGA+TGG | - | chr4.1:13125446-13125465 | MS.gene53366:CDS | 50.0% |
| !! | GGGTCTTTTGCTGGCTGAAA+AGG | + | chr4.1:13125486-13125505 | None:intergenic | 50.0% |
| CTCAGAACTCCAAGTGCAGC+TGG | - | chr4.1:13125549-13125568 | MS.gene53366:CDS | 55.0% | |
| TGGGCCCACAAGAGAAAACC+TGG | + | chr4.1:13125637-13125656 | None:intergenic | 55.0% | |
| ACTGGTGCACCAGCTGCACT+TGG | + | chr4.1:13125561-13125580 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 13125380 | 13125667 | 13125380 | ID=MS.gene53366 |
| chr4.1 | mRNA | 13125380 | 13125667 | 13125380 | ID=MS.gene53366.t1;Parent=MS.gene53366 |
| chr4.1 | exon | 13125380 | 13125667 | 13125380 | ID=MS.gene53366.t1.exon1;Parent=MS.gene53366.t1 |
| chr4.1 | CDS | 13125380 | 13125667 | 13125380 | ID=cds.MS.gene53366.t1;Parent=MS.gene53366.t1 |
| Gene Sequence |
| Protein sequence |
